Embodied CO2 emissions of equity portfolios for Chinese asset managers.

The 2015 Paris Agreement has set out the climate change target of limiting global warming to 1.5 °C, which poses a serious challenge to countries to reduce emissions. As the world's largest carbon emitter, promoting the realization of the "dual-carbon" goal is the key to realizing China's green transformation and high-quality development. Chinese asset managers play active roles in the capital market as an important channel of asset allocation. Currently, the vast majority of Chinese asset managers hold high percentages of high-carbon industries in their portfolios, and lack quantitative data of their carbon footprints embodied in equity investments, which faces huge carbon-related risks. Therefore, it's an urgent need to comprehensively and scientifically measure financed emissions of Chinese asset managers, which is of great significance for asset managers' carbon risk management and sustainable investment. This paper develops a detailed inventory of carbon emissions for equity portfolios managed by Chinese asset managers from 2010 to 2020, which stands as a pivotal reference for in-depth analysis of emission characteristics.

A regional cooperative reduction game model for air pollution control in North China.

Due to variations in economic scale, economic structure, and technological advancement across different Chinese provinces and cities, the cost of air pollution reduction differs significantly. Therefore, the total reduction cost can be decreased by capitalizing on these regional discrepancies in reduction cost to carry out cooperative emission reduction. In this paper, taking NOx reduction in North China as an example, a regional cooperative reduction game (CRG) model was constructed to minimize the total cost of emission reduction while achieving future emission reduction targets. The fair allocation of benefits from cooperation plays a crucial role in motivating regions to participate into the cooperation. A comprehensive mechanism of benefits allocation was proposed to achieve fair transferred compensation. The mechanism combines the consumption responsibility principle based on input-output theory and the Shapley value method based on game theory. Compared to the cost before the optimized collaboration, the CRG model will save 20.36% and 13.71% of the total reduction cost in North China, respectively, under the target of 17.68% NOx reduction by 2025 and 66.44% NOx reduction by 2035 relative to 2020. This method can be employed in other regions to achieve targets for air pollution reduction at minimum cost, and to motivate inter-regional cooperation with this practical and fair way of transferred compensation.

Leveraging opportunity of low carbon transition by super-emitter cities in China.

Chinese cities are core in the national carbon mitigation and largely affect global decarbonisation initiatives, yet disparities between cities challenge country-wide progress. Low-carbon transition should preferably lead to a convergence of both equity and mitigation targets among cities. Inter-city supply chains that link the production and consumption of cities are a factor in shaping inequality and mitigation but less considered aggregately. Here, we modelled supply chains of 309 Chinese cities for 2012 to quantify carbon footprint inequality, as well as explored a leverage opportunity to achieve an inclusive low-carbon transition. We revealed significant carbon inequalities: the 10 richest cities in China have per capita carbon footprints comparable to the US level, while half of the Chinese cities sit below the global average. Inter-city supply chains in China, which are associated with 80% of carbon emissions, imply substantial carbon leakage risks and also contribute to socioeconomic disparities. However, the significant carbon inequality implies a leveraging opportunity that substantial mitigation can be achieved by 32 super-emitting cities. If the super-emitting cities adopt their differentiated mitigation pathway based on affluence, industrial structure, and role of supply chains, up to 1.4 Gt carbon quota can be created, raising 30% of the projected carbon quota to carbon peak. The additional carbon quota allows the average living standard of the other 60% of Chinese people to reach an upper-middle-income level, highlighting collaborative mechanism at the city level has a great potential to lead to a convergence of both equity and mitigation targets.

Revealing Inter-regional Environmental Inequities Hidden in China's Energy Transition.

Energy transition is an important way to control air pollution, but it may conflict with the economic goal of alleviating regional inequality due to its inherently different cost burdens. As one of the effective measures of energy transition, this paper takes small coal-fired boiler (SCB) upgrading as an example to explore the regional mismatch between upgrading costs and health benefits. Here, we construct a boiler-level inventory of SCB upgrades for the North China Plain (NCP) during 2013-2017 and propose an integrated modeling framework to quantify the spatial contribution of economic costs and health benefits associated with SCB upgrading. We find that although the total health benefits could offset the total costs for the entire region, the developed municipalities (Beijing and Tianjin) are likely to gain more health benefits from less-developed neighboring provinces at lower costs. These developed municipalities contribute only 14% to the total health benefits but gain 21% of the benefits within their territories, 56% of which come from neighboring provinces. Their benefits are approximately 5.6 times their costs, which is much higher than the 1.5 benefit-cost ratio in neighboring provinces. Our findings may be useful in shaping more equitable and sound environmental policies in China or other regions of the world with serious coal-related air pollution.

The narrowing gap in developed and developing country emission intensities reduces global trade's carbon leakage.

International trade affects CO2 emissions by redistributing production activities to places where the emission intensities are different from the place of consumption. This study focuses on the net emission change as the result of the narrowing gap in emission intensities between the exporter and importer. Here we show that the relocation of production activities from the global North (developed countries) to the global South (developing countries) in the early 2000s leads to an increase in global emissions due to the higher emission intensities in China and India. The related net emissions are about one-third of the total emissions embodied in the South-North trade. However, the narrowing emission intensities between South-North and the changing trade patterns results in declining net emissions in trade in the past decade. The convergence of emission intensities in the global South alleviates concerns that increasing South-South trade would lead to increased carbon leakage and carbon emissions. The mitigation opportunity to green the supply chain lies in sectors such as electricity, mineral products and chemical products, but calls for a universal assessment of emission intensities and concerted effort.

Value chain carbon footprints of Chinese listed companies.

Measuring the value chain carbon footprints of listed companies is essential for cumulative climate actions and climate-efficient capital allocation. We trace the carbon emissions embodied in the value chains of Chinese listed companies and find that there is an increasing trend in terms of the carbon footprints of listed companies over the period 2010-2019. In 2019, the direct emissions from these companies reached 1.9 billion tonnes, accounting for 18.3% of national emissions. The indirect emissions were well over twice as large as the direct emissions from 2010 to 2019. Energy, construction and finance companies tend to have a greater volume of value chain carbon footprints, yet the distribution of their carbon footprints varies significantly. Finally, we apply the results to evaluate the financed emissions of leading asset managers' equity portfolio investment in China's stock market.

Consumption-based emissions at city level in China and the spatial heterogeneity analysis of the influential factors.

It is of great significance to identify the critical influential factors of pollutant emissions for emission mitigation. However, city disparity implies different priorities for regional mitigation. This study aims to estimate the consumption-based emissions of 309 prefecture-level cities in China based on the multi-region input-output table and the sectoral NOx emission inventory and investigate the emission transfer phenomenon among cities and sectors. In addition, a geographically weighted regression method is used to analyze the spatial heterogeneity in the driving factors of regional consumption-based emissions. The results reveal that the top 10 cities in consumption-based emissions account for 25.2% of emissions and contribute 22.6% to GDP. The consumption-based emissions are mainly driven by local demand (72.79%) at the regional level and by construction activities (94.43%) at the sectoral level. Besides, the results also show the spatial variances in contributions of driving forces to consumption-based emissions. Economic growth has been identified as the most important factor which promotes consumption-based emissions. However, disposable personal income, per capita road area, urbanization, and percentage of tertiary industry GDP are conducive to reduce consumption-based emissions in some cities of China. It could be concluded that policies without consideration of the emissions from a consumption perspective are difficult to achieve effective emission reduction.

Economic environmental imbalance in China - Inter-city air pollutant emission linkage in Beijing-Tianjin-Hebei (BTH) urban agglomeration.

Production fragmentation makes the air pollution policy no longer at the local scale but requires accounting more about embodied emissions cross-region through supply chains. Here, we map the consumption-driven NOx networks of Beijing-Tianjin-Hebei urban agglomeration (BTH) in China using the city-level multi-regional input-output model. The results show that the construction, service, and equipment manufacturing sectors in Beijing and Tianjin indirectly drive more than half of BTH NOx emissions (54%). Moreover, 75% of NOx flows in the supply chains are traded from cities with low efficiency (high intensity) to cities with high efficiency (low intensity), which reflects the economic environmental imbalance in BTH. Especially, for the metals smelting and pressing sector and nonmetal mineral products sector, there is a wider gap in emission intensity between production-oriented cities (1.03-4.43 Mt/million yuan) and consumer-oriented cities (0.08-0.45 Mt/million yuan), which leads to additional emissions of air pollutants to increase. At the same time, for the provinces in the south and north China, the role of BTH in the supply chain is different, which leads to an economic environmental imbalance between the north and the south. Synchronous outsourcing of production and technology is the key to solving the economic environmental imbalance. The consumption-oriented high-income cities are suggested to increase the financial and technical support to improve the efficiency of pollution control in production-oriented cities.

Transcriptome analysis reveals the genetic basis of skeletal muscle glycolytic potential based on a pig model.

Glycolytic potential (GP) calculated based on glucose, glycogen, glucose-6-phosphate, and lactate contents is a critical factor for multiple meat quality characteristics. However, the genetic basis of glycolytic metabolism is still unclear. In this study, we constructed six RNA-Seq libraries using longissimus dorsi (LD) muscles from pigs divergent for GP phenotypic values and generated the whole genome-wide gene expression profiles. Furthermore, we identified 25,880 known and 220 novel genes from these skeletal muscle libraries, and 222 differentially expressed genes (DEGs) between the higher and lower GP groups. Notably, we found that the Lactate dehydrogenase B (LDHB) and Fructose-2, 6-biphosphatase 3 (PFKFB3) expression levels were higher in the higher GP group than the lower GP group, and positively correlated with GP and lactic acid (LA), and reversely correlated with pH value at 45 min postmortem (pH45min). Besides, LDHB and PFKFB3 expression were positively correlated with drip loss measured at 48 h postmortem (DL48h) and drip loss measured at 24 h postmortem (DL24h). Collectively, we identified a serial of DEGs as the potential key candidate genes affecting GP and found that LDHB and PFKFB3 are closely related to GP and GP-related traits. Our results lay a solid basis for in-depth studies of the regulatory mechanisms on GP and GP-related traits in pigs.

Exploring the lncRNAs Related to Skeletal Muscle Fiber Types and Meat Quality Traits in Pigs.

The alteration in skeletal muscle fiber is a critical factor affecting livestock meat quality traits and human metabolic diseases. Long non-coding RNAs (lncRNAs) are a diverse class of non-coding RNAs with a length of more than 200 nucleotides. However, the mechanisms underlying the regulation of lncRNAs in skeletal muscle fibers remain elusive. To understand the genetic basis of lncRNA-regulated skeletal muscle fiber development, we performed a transcriptome analysis to identify the key lncRNAs affecting skeletal muscle fiber and meat quality traits on a pig model. We generated the lncRNA expression profiles of fast-twitch Biceps femoris (Bf) and slow-twitch Soleus (Sol) muscles and identified the differentially expressed (DE) lncRNAs using RNA-seq and performed bioinformatics analyses. This allowed us to identify 4581 lncRNA genes among six RNA libraries and 92 DE lncRNAs between Bf and Sol which are the key candidates for the conversion of skeletal muscle fiber types. Moreover, we detected the expression patterns of lncRNA MSTRG.42019 in different tissues and skeletal muscles of various development stages. In addition, we performed a correlation analyses between the expression of DE lncRNA MSTRG.42019 and meat quality traits. Notably, we found that DE lncRNA MSTRG.42019 was highly expressed in skeletal muscle and its expression was significantly higher in Sol than in Bf, with a positive correlation with the expression of Myosin heavy chain 7 (MYH7) (r = 0.6597, p = 0.0016) and a negative correlation with meat quality traits glycolytic potential (r = -0.5447, p = 0.0130), as well as drip loss (r = -0.5085, p = 0.0221). Moreover, we constructed the lncRNA MSTRG.42019-mRNAs regulatory network for a better understanding of a possible mechanism regulating skeletal muscle fiber formation. Our data provide the groundwork for studying the lncRNA regulatory mechanisms of skeletal muscle fiber conversion, and given the importance of skeletal muscle fiber types in muscle-related diseases, our data may provide insight into the treatment of muscular diseases in humans.

Linkage analysis of economic consumption, pollutant emissions and concentrations based on a city-level multi-regional input-output (MRIO) model and atmospheric transport.

China is experiencing serious atmospheric pollution, which also exhibits significant spatial heterogeneity. The Chinese government has implemented targeted pollution control measures at the city level, emphasizing coordination among cities to prevent and control air pollution in key regions such as Beijing-Tianjin-Hebei (BTH) urban agglomeration. This study combined an inter-city multi-regional input-output (MRIO) model with an air quality dispersion model consisting of a weather research and forecasting (WRF) model and the CALPUFF model (WRF/CALPUFF) to study the inter-city economic consumption, pollutant emission and concentration among 13 cities in BTH urban agglomeration. NOx is chosen as an example. The combined effects of economic linkage and atmospheric transport show that NOx concentrations in cities in the BTH urban agglomeration are attributable to three consumption sources: a local contribution from the target city's own local economic consumption (average, 25%), and non-local consumption contributions, including other cities in the BTH urban agglomeration (average, 36%) and regions outside of BTH (average, 39%). Compared with the contributions to NOx concentrations calculated using only the MRIO model or atmospheric transport stimulation model, the results of this paper quantify that the consumption outside of a city could provide a greater impact on the city's air quality due to the combined effects of economic linkage and atmospheric transport. To avoid negative impacts of emission reduction targets on economic consumption, governmental regional pollution control policies should consider the combined effects of economic linkage and atmospheric transport.

Effect of MSTN Mutation on Growth and Carcass Performance in Duroc x Meishan Hybrid Population.

The Meishan pig is a traditional Chinese native breed, known for its excellent reproduction performance that is widely used in commercial pig production through two-way or three-way crossbreeding systems. However, the lean meat yield of Meishan crossbred pigs is still very low and cannot meet the market demand. To evaluate the lean meat yield of Meishan crossbred pigs, six wild-type Meishan sows were artificially inseminated by using the MSTN+/- Duroc boar semen in this experiment. Some reproductive performance-related traits of Meishan sows were recorded to ensure that semen from MSTN knockout Duroc boar did not affect offspring production, including total births, live births, sex, and litter weight. In total, 73 piglets were obtained and 63 were alive. Male to female ratio was close to 1: 1. because of factors such as disease, only 43 pigs were utilized, including 28 MSTN mutant pigs (MSTN+/-) and 15 MSTN homozygous pigs (MSTN+/+). We compared the growth performance and carcass performance of these full or half-sib populations and found that there were no differences between MSTN+/- and MSTN+/+ genotypes for live animal measures including average daily gain (ADG), body dimensions, or ultrasonic measurement of fat thickness when pigs were harvested after 120 days of feeding. Conversely, the MSTN+/- pigs had higher dressing percentage and lean meat percentage, lower level of carcass fat, larger longissimus muscle area, less percentage of skin and skeleton, thinner average backfat thickness, and lower intramuscular fat (IMF) content than MSTN+/+ pigs. In conclusion, the production of MSTN+/- mutant progeny from Meishan females resulted in improved carcass composition, providing a feasible solution to improve the lean meat yield of Chinese local fat-type pig breeds.

Profiling and Functional Analysis of Circular RNAs in Porcine Fast and Slow Muscles.

The different skeletal muscle fiber types exhibit distinctively different physiological and metabolic properties, and have been linked to both human metabolic diseases and meat quality traits in livestock. Circular RNAs (circRNAs) are a new class of endogenous RNA regulating gene expression, but regulatory mechanisms of skeletal muscle fibers involved in circRNAs remain poorly understood. Here, we constructed circRNA expression profiles of three fast-twitch biceps femoris (Bf) and three slow-twitch soleus (Sol) muscles in pigs using RNA-seq and identified 16,342 distinct circRNA candidates. Notably, 242 differentially expressed (DE) circRNAs between Bf and Sol muscles were identified, including 105 upregulated and 137 downregulated circRNAs, and are thus potential candidates for the regulation of skeletal muscle fiber conversion. Moreover, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of host genes of DE circRNAs revealed that host genes were mainly involved in skeletal muscle fiber-related GO terms (e.g., muscle contraction, contractile fiber part, and Z disk) and skeletal muscle fiber-related signaling pathways (e.g., AMPK and cGMP-PKG). We also constructed co-expression networks of DE circRNA-miRNA-mRNA using previously acquired high-throughput sequencing mRNA and miRNA data, from which 112 circRNA-miRNA and 95 miRNA-mRNA interactions were identified. Multiple circRNAs essentially serve as a sponge for miR-499-5p, which is preferentially expressed in slow-twitch muscle and reduces the severity of Duchenne muscular dystrophy (DMD). Taken together, a series of novel candidate circRNAs involved in the growth and development of porcine skeletal muscle was identified. Furthermore, they provide a comprehensive circRNA resource for further in-depth research on the regulatory mechanisms of circRNA in the formation of skeletal muscle fiber, and may provide insights into human skeletal muscle diseases.

Re-examining the embodied air pollutants in Chinese exports.

China is the world's largest exporter and may release lots of air pollutants to produce exported commodities due to taking coal as its main source of energy. Processing exports play a significant role in Chinese exports, yet previous studies of embodied air pollutants in Chinese exports failed to distinguish processing exports from normal exports. This paper investigates the effect of trade heterogeneity on the estimation of embodied emissions by re-examining the embodied air pollutants in Chinese exports based on an extended non-competitive input-output table that distinguishes processing from normal exports. The results show that processing exports generate 22.81% of the value added embodied in gross exports and 16.48% of the emissions embodied in gross exports. The embodied air pollutants in Chinese exports would be overestimated by 12%-22% without accounting for trade heterogeneity. Unequal distributions of export-related air pollutants and value added exist among different sectors. In particular, Manufacturing of Electronics and Communication Equipment sector induces 39.56% of embodied emissions in processing exports, and 41.78% of which are generated by Production and Supply of Electric Power and Steam Hot Water sector. China's restrictions on processing exports should focus not only on the direct emissions generated by each sector but also on the emissions embodied in domestic supply chains.

MicroRNA-206 regulates cell proliferation by targeting G6PD in skeletal muscle.

Skeletal muscle is a major component of body mass and plays a central role in the control of whole-body metabolism in humans and animals. Therefore, elucidation of the underlying mechanisms of skeletal growth and development are expected to lead to the discovery of novel genes and pathways related to muscle disease. miR-206, a skeletal muscle-specific microRNA, plays a crucial role in myogenesis; however, miR-206 is known to function in myogenic differentiation, whether or not it affects muscle cells' proliferation, and the underlying mechanisms are unknown. In this study, we investigated the effect of miR-206 on muscle cell proliferation and differentiation, as well as its effect on myofiber type conversion using mouse C2C12 myoblasts. The results showed that overexpression of miR-206 inhibited cell proliferation and promoted muscle cell differentiation, but it did not affect myofiber type conversion. Intriguingly, we found that overexpression of miR-206 suppressed muscle cell proliferation and induced cell cycle arrest in G0/G1 phase by inhibiting the glucose-6-phosphate dehydrogenase (G6PD) gene. Taken together, we demonstrated that the miR-206-G6PD pathway suppresses muscle cell proliferation, and these findings may facilitate the treatment of muscle diseases.-Jiang, A., Dong, C., Li, B., Zhang, Z., Chen, Y., Ning, C., Wu, W., Liu, H. MicroRNA-206 regulates cell proliferation by targeting G6PD in skeletal muscle.

Impact of a Coal-Fired Power Plant Shutdown Campaign on Heavy Metal Emissions in China.

Recently, China has committed to decommissioning the heavy metal (HM) intensive coal-fired power plants (CFPPs), small units especially, yet a quantitative assessment for the impact on HM emissions remains poorly understood. This study, for the first time, compiles a plant-specific inventory for six HMs (Hg, As, Se, Pb, Cd, and Cr) avoided by CFPPs decommissioned in China during the 12th Five Year Plan period. The reduced HM amounts to 271.58 t (9.19 t Hg, 45.84 t As, 60.76 t Se, 85.30 t Pb, 1.74 t Cd, and 68.75 t Cr), accounting for 12.71% of the total emissions from all China's CFPPs in 2010. Small units which have low boiler efficiency and lack air pollutant control devices are more than tenfold HM-intensive as the large units. The detailed HM emission factors for each CFPP decommissioned in each provincial region are also identified. HM content in the coal consumed is a key parameter to determine their corresponding emission factors, while the capacity of decommissioned coal plants plays a decisive role in the reduced emissions. The high-resolution inventory not only verifies China's progress in alleviating HM pollution, but also provides basis for further investigation into HM relevant environmental and human health impact.

Can virtual water trade save water resources?

At times, certain areas of China suffering from water shortages. While China's government is spurring innovation and infrastructure to help head off such problems, it may be that some water conservation could help as well. It is well-known that water is embodied in traded goods-so called "virtual water trade" (VWT). In China, it seems that many water-poor areas are perversely engaged in VWT. Further, China is engaging in the global trend of fragmentation in production, even as an interregional phenomenon. Perhaps something could be learned about conserving or reducing VWT, if we knew where and how it is practiced. Given some proximate causes, perhaps viable policies could be formulated. To this end, we employ China's multiregional input-output tables straddling two periods to trace the trade of a given region's three types of goods: local final goods, local intermediate goods, and goods that shipped to other regions and countries. We find that goods traded interregionally in China in 2012 embodied 30.4% of all water used nationwide. Nationwide, water use increased substantially over 2007-2012 due to greater shipment volumes of water-intensive products. In fact, as suspected, the rise in value chain-related trade became a major contributing factor. Coastal areas tended to be net receivers of VWT from interior provinces, although reasons differed, e.g. Shanghai received more to fulfill final demand (67.8% of net inflow) and Zhejiang for value-chain related trade (40.2% of net inflow). In sum, the variety of our findings reveals an urgent need to consider trade types and water scarcity when developing water resource allocation and conservation policies.

Identification of a novel miR-206-Notch3 pathway regulating mouse myoblasts proliferation.

MicroRNAs (miRNAs) are endogenous short non-coding RNAs and exert their function by targeting mRNAs of genes. miRNA-206 (miR-206) is exclusively expressed in adult skeletal muscles and plays an important role in myogenesis. However, the regulatory mechanisms of miR-206 in myoblasts proliferation and differentiation are still limited. In this study, we validated that Notch3 is a direct target gene of mouse miR-206 using dual-luciferase reporter assay and miR-206-overexpressed experiments. Furthermore, we demonstrated that the negative effect of overexpression of miR-206 and knockdown of Notch3 on C2C12 cells proliferation, and found that the effect was produced by controlling the transition of G0/G1 and S phase in C2C12 cell cycle. In summary, our results provide direct evidences that miR-206 regulates skeletal muscle cells proliferation and cell cycle arrest partly by targeting the Notch3 gene, which strengthens our understanding of regulatory mechanisms on miR-206 in skeletal muscle growth and development.

A Key Gene, PLIN1, Can Affect Porcine Intramuscular Fat Content Based on Transcriptome Analysis.

Intramuscular fat (IMF) content is an important indicator for meat quality evaluation. However, the key genes and molecular regulatory mechanisms affecting IMF deposition remain unclear. In the present study, we identified 75 differentially expressed genes (DEGs) between the higher (H) and lower (L) IMF content of pigs using transcriptome analysis, of which 27 were upregulated and 48 were downregulated. Notably, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that the DEG perilipin-1 (PLIN1) was significantly enriched in the fat metabolism-related peroxisome proliferator-activated receptor (PPAR) signaling pathway. Furthermore, we determined the expression patterns and functional role of porcine PLIN1. Our results indicate that PLIN1 was highly expressed in porcine adipose tissue, and its expression level was significantly higher in the H IMF content group when compared with the L IMF content group, and expression was increased during adipocyte differentiation. Additionally, our results confirm that PLIN1 knockdown decreases the triglyceride (TG) level and lipid droplet (LD) size in porcine adipocytes. Overall, our data identify novel candidate genes affecting IMF content and provide new insight into PLIN1 in porcine IMF deposition and adipocyte differentiation.

The c.-360 T>C mutation affects PGAM2 transcription activity and is linked with the water holding capacity of the longissimus lumborum muscle in pigs.

The phosphoglycerate mutase 2 (PGAM2) gene encodes a key enzyme in the glycolytic process. This study examined a functional mutation in the PGAM2 gene and evaluated its relationship with water holding capacity (WHC). RT-qPCR analysis showed the PGAM2 mRNA level was significantly higher in the low-WHC group than in the high-WHC group (P<0.05). The c.-360 T>C mutation was identified through sequencing and found to have opposite allele distributions in the two groups. The allele was further genotyped in 170 Duroc×Large White×Yorkshire crossbred pigs using allele-specific PCR. The CC genotype was associated with lower WHC and higher PGAM2 mRNA levels, whereas the TT genotype corresponded to a higher WHC and lower PGAM2 mRNA levels (P<0.05). A luciferase activity assay also showed that the CC-genotype promoter had higher activity than the TT-genotype promoter (P<0.05). In conclusion, we discovered the c.-360 T>C mutation in the PGAM2 gene, which is a promising marker for improving pork WHC.