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BACKGROUND: Atherosclerosis (AS) is commonly regarded as a key driver accounted for the leading causes of morbidity and mortality among cardiovascular and cerebrovascular diseases. A growing body of evidence indicates that autophagy in macrophages involved in AS might be a potential therapeutic target. C1q/TNF-related protein 9 (CTRP9) has been proven to delay the progression of cardiovascular diseases. However, the relations between CTRP9 and Sirt1, as well as their effects on macrophages autophagy have not been fully explored. METHODS: Macrophages were differentiated from mononuclear cells collected from peripheral blood samples of healthy donors. The in vitro AS models were constructed by ox-LDL treatment. Cell viability was determined by CCK-8 assay. Immunofluorescence assay of LC3 was implemented for evaluating autophagy activity. Oil Red O staining was performed for lipid accumulation detection. ELISA, cholesterol concentration assay and cholesterol efflux analysis were conducted using commercial kits. Cycloheximide assay was implemented for revealing protein stability. RT-qPCR was used for mRNA expression detection, and western blotting was performed for protein level monitoring. RESULTS: CTRP9 attenuated impaired cell viability, autophagy inhibition and increased lipid accumulation induced by ox-LDL. Moreover, CTRP9 maintained Sirt1 protein level through enhancing its stability through de-ubiquitination, which was mediated by upregulated USP22 level. CRTP9 exerted its protective role in promoting autophagy and reducing lipid accumulation through the USP22/Sirt1 axis. CONCLUSION: Collectively, CTRP9 alleviates lipid accumulation and facilitated the macrophages autophagy by upregulating USP22 level and maintaining Sirt1 protein expression, thereby exerting a protective role in AS progression in vitro.
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Aterosclerosis , Sirtuina 1 , Humanos , Sirtuina 1/genética , Sirtuina 1/metabolismo , Complemento C1q/genética , Complemento C1q/metabolismo , Complemento C1q/farmacología , Macrófagos/metabolismo , Lipoproteínas LDL/farmacología , Colesterol/metabolismo , Aterosclerosis/metabolismo , Autofagia , UbiquitinaciónRESUMEN
ADAPTOR-ASSOCIATED PROTEIN KINASE1 (AAK1) is a known regulator of clathrin-mediated endocytosis in mammals. Human AAK1 phosphorylates the µ2 subunit of the ADAPTOR PROTEIN-2 (AP-2) complex (AP2M) and plays important roles in cell differentiation and development. Previous interactome studies discovered the association of AAK1 with AP-2 in Arabidopsis (Arabidopsis thaliana), but its function was unclear. Here, genetic analysis revealed that the Arabidopsis aak1 and ap2m mutants both displayed altered root tropic growth, including impaired touch- and gravity-sensing responses. In Arabidopsis, AAK1-phosphorylated AP2M on Thr-163, and expression of the phospho-null version of AP2M in the ap2m mutant led to an aak1-like phenotype, whereas the phospho-mimic forms of AP2M rescued the aak1 mutant. In addition, we found that the AAK1-dependent phosphorylation state of AP2M modulates the frequency distribution of endocytosis. Our data indicate that the phosphorylation of AP2M on Thr-163 by AAK1 fine-tunes endocytosis in the Arabidopsis root to control its tropic growth.
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Subunidades mu de Complejo de Proteína Adaptadora , Arabidopsis , Raíces de Plantas , Animales , Humanos , Complejo 2 de Proteína Adaptadora/genética , Complejo 2 de Proteína Adaptadora/metabolismo , Subunidades mu de Complejo de Proteína Adaptadora/metabolismo , Arabidopsis/metabolismo , Clatrina/metabolismo , Endocitosis/genética , Mamíferos/metabolismo , Fosforilación , Proteínas Serina-Treonina Quinasas/genética , Proteínas Serina-Treonina Quinasas/metabolismo , Raíces de Plantas/genética , Raíces de Plantas/metabolismoRESUMEN
Adaptor protein (AP) complexes are evolutionarily conserved vesicle transport regulators that recruit coat proteins, membrane cargoes and coated vesicle accessory proteins. As in plants endocytic and post-Golgi trafficking intersect at the trans-Golgi network, unique mechanisms for sorting cargoes of overlapping vesicular routes are anticipated. The plant AP complexes are part of the sorting machinery, but despite some functional information, their cargoes, accessory proteins and regulation remain largely unknown. Here, by means of various proteomics approaches, we generated the overall interactome of the five AP and the TPLATE complexes in Arabidopsis thaliana. The interactome converged on a number of hub proteins, including the thus far unknown adaptin binding-like protein, designated P34. P34 interacted with the clathrin-associated AP complexes, controlled their stability and, subsequently, influenced clathrin-mediated endocytosis and various post-Golgi trafficking routes. Altogether, the AP interactome network offers substantial resources for further discoveries of unknown endomembrane trafficking regulators in plant cells.
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Proteínas de Arabidopsis , Arabidopsis , Arabidopsis/metabolismo , Proteínas de Arabidopsis/metabolismo , Red trans-Golgi/metabolismo , Aparato de Golgi/metabolismo , Clatrina/metabolismoRESUMEN
In this paper, a deep learning-based traffic state discrimination method is proposed to detect traffic congestion at urban intersections. The detection algorithm includes two parts, global speed detection and a traffic state discrimination algorithm. Firstly, the region of interest (ROI) is selected as the road intersection from the input image of the You Only Look Once (YOLO) v3 object detection algorithm for vehicle target detection. The Lucas-Kanade (LK) optical flow method is employed to calculate the vehicle speed. Then, the corresponding intersection state can be obtained based on the vehicle speed and the discrimination algorithm. The detection of the vehicle takes the position information obtained by YOLOv3 as the input of the LK optical flow algorithm and forms an optical flow vector to complete the vehicle speed detection. Experimental results show that the detection algorithm can detect the vehicle speed and traffic state discrimination method can judge the traffic state accurately, which has a strong anti-interference ability and meets the practical application requirements.
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Motivated by the fact that the medial axis transform is able to encode the shape completely, we propose to use as few medial balls as possible to approximate the original enclosed volume by the boundary surface. We progressively select new medial balls, in a top-down style, to enlarge the region spanned by the existing medial balls. The key spirit of the selection strategy is to encourage large medial balls while imposing given geometric constraints. We further propose a speedup technique based on a provable observation that the intersection of medial balls implies the adjacency of power cells (in the sense of the power crust).We further elaborate the selection rules in combination with two closely related applications. One application is to develop an easy-to-use ball-stick modeling system that helps non-professional users to quickly build a shape with only balls and wires, but any penetration between two medial balls must be suppressed. The other application is to generate porous structures with convex, compact (with a high isoperimetric quotient) and shape-aware pores where two adjacent spherical pores may have penetration as long as the mechanical rigidity can be well preserved.
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Clathrin-mediated endocytosis (CME) and its core endocytic machinery are evolutionarily conserved across all eukaryotes. In mammals, the heterotetrameric adaptor protein complex-2 (AP-2) sorts plasma membrane (PM) cargoes into vesicles via the recognition of motifs based on Tyr or di-Leu in their cytoplasmic tails. However, in plants, very little is known about how PM proteins are sorted for CME and whether similar motifs are required. In Arabidopsis (Arabidopsis thaliana), the brassinosteroid (BR) receptor BR INSENSITIVE1 (BRI1) undergoes endocytosis, which depends on clathrin and AP-2. Here, we demonstrate that BRI1 binds directly to the medium AP-2 subunit (AP2M). The cytoplasmic domain of BRI1 contains five putative canonical surface-exposed Tyr-based endocytic motifs. The Tyr-to-Phe substitution in Y898KAI reduced BRI1 internalization without affecting its kinase activity. Consistently, plants carrying the BRI1Y898F mutation were hypersensitive to BRs. Our study demonstrates that AP-2-dependent internalization of PM proteins via the recognition of functional Tyr motifs also operates in plants.
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Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/metabolismo , Endocitosis/fisiología , Proteínas Quinasas/química , Proteínas Quinasas/metabolismo , Secuencias de Aminoácidos , Arabidopsis/genética , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Membrana Celular/metabolismo , Proteínas Fluorescentes Verdes/genética , Mutación , Plantas Modificadas Genéticamente , Dominios Proteicos , Proteínas Quinasas/genética , Tirosina/químicaRESUMEN
Two organic molecules, pyridoxal hydrazide (PAH) and salicylaldehyde based Rhodamine B hydrazone (RBS) were integrated into zeolitic imidazolate framework-8 (ZIF-8) to give composites, namely PAH/ZIF-8 and RBS/ZIF-8. The organic molecules and ZIF-8 are proposed to be assembled via hydrogen bonds and π-π stacking in the composites. The mass fraction of PAH and RBS in the composites was calculated to be 21.86% and 29.3%. The fluorescence of PAH/ZIF-8 is quenched regularly by Cu2+. The detection limit for Cu2+ was calculated to be 1.42 nM for PAH/ZIF-8, which is one order of magnitude lower than that of PAH. The detection limit for Cu2+ was determined to be 0.8 µM for RBS/ZIF-8, which is three times lower than that of RBS. The two composites both display high selectivity to Cu2+ over competing metal ions. The PAH/ZIF-8 fluorescent sensor was successfully applied to Cu2+ determination in environmental water. PAH/ZIF-8 exhibits excellent cell membrane permeability and low cytotoxicity in cellular imaging. The enhanced chemical sensor was designed by introducing small molecules into ZIF-8 for the specific recognition of Cu2+.
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The Arabidopsis thaliana endogenous elicitor peptides (AtPeps) are released into the apoplast after cellular damage caused by pathogens or wounding to induce innate immunity by direct binding to the membrane-localized leucine-rich repeat receptor kinases, PEP RECEPTOR1 (PEPR1) and PEPR2. Although the PEPR-mediated signaling components and responses have been studied extensively, the contributions of the subcellular localization and dynamics of the active PEPRs remain largely unknown. We used live-cell imaging of the fluorescently labeled and bioactive pep1 to visualize the intracellular behavior of the PEPRs in the Arabidopsis root meristem. We found that AtPep1 decorated the plasma membrane (PM) in a receptor-dependent manner and cointernalized with PEPRs. Trafficking of the AtPep1-PEPR1 complexes to the vacuole required neither the trans-Golgi network/early endosome (TGN/EE)-localized vacuolar H(+)-ATPase activity nor the function of the brefeldin A-sensitive ADP-ribosylation factor-guanine exchange factors (ARF-GEFs). In addition, AtPep1 and different TGN/EE markers colocalized only rarely, implying that the intracellular route of this receptor-ligand pair is largely independent of the TGN/EE. Inducible overexpression of the Arabidopsis clathrin coat disassembly factor, Auxilin2, which inhibits clathrin-mediated endocytosis (CME), impaired the AtPep1-PEPR1 internalization and compromised AtPep1-mediated responses. Our results show that clathrin function at the PM is required to induce plant defense responses, likely through CME of cell surface-located signaling components.
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Arabidopsis/metabolismo , Clatrina/metabolismo , Péptidos/metabolismo , Transducción de Señal , Proteínas de Arabidopsis/metabolismo , Membrana Celular/metabolismo , Endocitosis , Endosomas/metabolismo , Proteínas Fluorescentes Verdes/metabolismo , Meristema/citología , Meristema/metabolismo , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Modelos Biológicos , Proteínas Serina-Treonina Quinasas/metabolismo , Receptores de Superficie Celular/metabolismo , Rodaminas/metabolismo , Fracciones Subcelulares/metabolismo , ATPasas de Translocación de Protón Vacuolares/metabolismo , Red trans-Golgi/metabolismoRESUMEN
Heterogeneous nuclear ribonucleoproteins (hnRNPs) participate in diverse regulations of plant growth and environmental stress responses. In this work, an Arabidopsis hnRNP of unknown function, AtRNP1, was investigated. We found that AtRNP1 gene is highly expressed in rosette and cauline leaves, and slightly induced under drought, salt, osmotic and ABA stresses. AtRNP1 protein is localized to both the nucleus and cytoplasm. We performed homologous overexpression of AtRNP1 and found that the transgenic plants showed shortened root length and plant height, and accelerated flowering. In addition, the transgenic plants also showed reduced tolerance to drought, salt, osmotic and ABA stresses. Further studies revealed that under both normal and stress conditions, the proline contents in the transgenic plants are markedly decreased, associated with reduced expression levels of a proline synthase gene and several stress-responsive genes. These results suggested that the overexpression of AtRNP1 negatively affects plant growth and abiotic stress tolerance.