Facile Synthesis of Spinel CoFe2O4 Nanoparticle and Its Application as Magnetic Recoverable Photocatalyst for Degradation of Metronidazole and Some Selected Organic Dyes.

A novel and facile approach for one-pot synthesis of spinel cobalt ferrites (CoFe2O4) nanoparticle (NPs) and is photocatalytic activity for degradation of pharmaceuticals waste and organic dye. The synthesis involves homogeneous chemical precipitation followed by hydrothermal heating, using of hexamine as a hydroxylating agent. As-synthesized CoFe2O4 photocatalyst was characterised by XRD, SEM, SEM Mapping, TEM, XPS, and BET-surface area analysis. The TEM image reveals cubic shapes with an average size of 10-20 nm. The surface area of the CoFe2O4 NPs was found to be 16.42 m² g-1. The photocatalytic activity of CoFe2O4 has proved to be an excellent photocatalyt for degradation of metronidazole and organic dyes such as methylene blue (MB) and rhodamine B (RhB). The rate of the reaction was found to be 0.102, 0.198 and 0.213 min-1 for metronidazole, MB and RhB respectively. The catalyst also proved to be noteworthy as it does not loss in its catalytic activity even after five cycles of reuse.

The specific cytotoxic effect of tumor infiltrating lymphocytes transfected with chimeric T cell receptor on cells which express KDR / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To investigate the specific cytotoxity of tumor infiltrating lymphocytes (TIL) transfected with chimeric T cell receptor (CTCR) on cells which express KDR.</p><p><b>METHODS</b>A recombinant retroviral plasmid (pMSCVneo-Vhgamma) was constructed by cloning VEGF121-hinger-FcRgamma (Vhgamma) into retroviral vector pMSCVneo. After packaging by PT67, the virus with high titer was used to infect TIL isolated from liver cancer tissues, and then MSCVneo-Vhgamma-TIL was generated. TIL infected with MSCVneo was used as a control. The cytotoxicty of the transgenic TIL on NIH3T3 and HepG2 expressing no KDR and on ECV304 and A375 expressing KDR was detected with MTT colorimetric assay.</p><p><b>RESULTS</b>The sequences of VEGF121 and hinger-FcRgamma were different from those reported, but the deduced amino sequences were identical to the reported ones. The cytotoxity of TIL infected with MSCVneo on target cell was similar to that of the control TIL; both only had mild cytotoxity on cancer cell line. No significant cytotoxity was found in TIL infected with MSCVneo-cTCR on NIH3T3, but its cytotoxity on ECV304 was significant. The cytotoxity on HepG2 was similar to that of MSCVneo-TIL and uninfected TIL, but cytotoxity on A375 was significantly higher.</p><p><b>CONCLUSION</b>Chimeric T cell receptor permanently grafts TIL cell with predefined new specificity. TIL expressing Vhgamma can selectively recognize and kill vascular endothelial cell and tumor cells which express VEGF receptor KDR.</p>

Correlation between serum alpha-fetoprotein variant levels in patients with hepatocellular carcinoma and the presence of alpha-fetoprotein mRNA in the circulating blood / 中华肝脏病杂志

<p><b>OBJECTIVE</b>To investigate the relationship between serum alpha-fetoprotein (AFP) variant levels in patients with hepatocellular carcinoma (HCC) and cancer cells disseminating through blood.</p><p><b>METHODS</b>Serum AFP variant levels were measured by crossed immunoaffino-electrophoresis in the presence of lectin before initial surgical treatment in HCC patients. Circulating tumor cells were simultaneously detected in pre-operative blood samples using reverse transcription-polymerase chain reaction (RT-PCR) for AFP mRNA.</p><p><b>RESULTS</b>Forty-six HCC patients with serum AFP positive were studied. Serum AFP variant level > or 20% was showed in 37 patients, among whom there were 22 (59.5%) showing AFP mRNA positive. In contrast, the positive AFP mRNA expression was only observed in 2 out of 9 patients (22.2%) with AFP variant level<20% (x(2)=4.02, P<0.05).</p><p><b>CONCLUSION</b>In hepatocellular carcinoma patients, increased AFP variant levels are associated with a haematogenous spread of tumor cells.</p>