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1.
Eur J Pharmacol ; 897: 173927, 2021 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-33567320

RESUMEN

Rotavirus (RV) infection is a leading cause of severe, dehydrating gastroenteritis in children < 5 years of age, and by now, the prevention and treatment of RV are still the major public health problems due to a lack of specific clinical drugs. Thus, the aims of this study are to explore the anti-RV effect of baicalin and its influence on glucose metabolism. Here, we demonstrated for the first time that baicalin had an anti-RV attachment effect with the strongest effect at a concentration of 100 µM, and also inhibited the replication of RV at concentrations of 100, 125, 150, 175, and 200 µM. Moreover, baicalin helped to overcome the weight loss and reduced the diarrhea rate and score with the best therapeutic effect at a concentration of 0.3 mg/g in RV-infected neonatal mice. Interestingly, baicalin decreased glucose consumption in RV-infected Caco-2 cells with the optimal concentration of 125 µM. Next, metabolomic analysis indicated that there were 68 differentially expressed metabolites, including an increase in pyruvic acid, asparagine, histidine and serine, and a decrease in dihydroxyacetone phosphate, which suggested that the underlying signaling pathway was gluconeogenesis. Further studies demonstrated that baicalin inhibited gluconeogenesis via improving glucose 6-phosphatase (G-6-Pase) and phosphoenolpyruvate carboxylase (PEPCK). Moreover, baicalin upregulated the potential gluconeogenesis proteins named salt inducible kinase 2, pyruvate dehydrogenase kinase 1, AKT serine/threonine kinase 1 and down-regulated phosphorylated c-Jun NH2-terminal kinase, which are associated with G-6-Pase and PEPCK expressions. Therefore, baicalin improved the gluconeogenesis disruption caused by RV.


Asunto(s)
Antivirales/farmacología , Flavonoides/farmacología , Gluconeogénesis/efectos de los fármacos , Proteínas Quinasas JNK Activadas por Mitógenos/metabolismo , Proteínas Serina-Treonina Quinasas/metabolismo , Proteínas Proto-Oncogénicas c-akt/metabolismo , Piruvato Deshidrogenasa Quinasa Acetil-Transferidora/metabolismo , Infecciones por Rotavirus/tratamiento farmacológico , Rotavirus/efectos de los fármacos , Animales , Células CACO-2 , Modelos Animales de Enfermedad , Interacciones Huésped-Patógeno , Humanos , Metaboloma , Metabolómica , Ratones , Fosforilación , Rotavirus/patogenicidad , Infecciones por Rotavirus/enzimología , Infecciones por Rotavirus/virología , Transducción de Señal , Acoplamiento Viral/efectos de los fármacos , Replicación Viral/efectos de los fármacos
2.
Asian J Pharm Sci ; 14(4): 413-422, 2019 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-32104470

RESUMEN

Time-sensitive and pH-dependent polymers are generally employed to prepare colon-site delivery system, and their coating thickness and order are very important in controlling the drug release. The traditional colon-site delivery systems consist of time-dependent polymers as inner layer and pH-sensitive polymers as outer layer. However, they suffer from low drug-loading rate and immature drug release. In this study, total alkaloids of sophora alopecuroides(TASA)-loaded pellets were prepared by extrusion-spheronization method and coated with Eudragit RS30D and Eudragit S100. Pellets using Eudragit RS30D as inner layer and Eudragit S100 as outer layer were named as ERS-ES100 TCO, while pellets with Eudragit S100 as inner layer and Eudragit RS30D as outer layer were ES100-ERS NCO. Both types of formulations with varying coating ratios and orders of Eudragit S100 and Eudragit RS30D were designed and prepared. The following in vitro drug release and SEM studies indicated that ERS-ES100 TCO(F2) with 12.8% Eudragit RS30D as inner layer and 21% Eudragit S100 as outer layer released up to 42% drug in 5 h. Interestingly, ES100-ERS NCO (F4) coated with 12.8% Eudragit S100 and 14.8% Eudragit RS30D showed optimal drug release in colon. In conclusion, ES100-ERS NCO colonic delivery system achieved reduced coating thickness and improved colonic targeting compared with traditional delivery system (ERS-ES100 TCO). In addition, the similarity factors (f2 ) value of sophoridine and matrine for investigated formulation were within 50-100 and > 80, demonstrating that sophoridine and matrine in all formulations achieved a synchronous release.

3.
Virus Genes ; 53(3): 367-376, 2017 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-28289928

RESUMEN

Rotavirus (RV) is the most common cause of severe gastroenteritis and fatal dehydration in human infants and neonates of different species. However, the pathogenesis of rotavirus-induced diarrhea is poorly understood. Secretory diarrhea caused by rotavirus may lead to a combination of excessive secretion of fluid and electrolytes into the intestinal lumen. Fluid absorption in the small intestine is driven by Na+-coupled transport mechanisms at the luminal membrane, including Na+/H+ exchanger (NHE). Here, we performed qRT-PCR to detect the transcription of NHEs. Western blotting was employed for protein detection. Furthermore, immunocytochemistry was used to validate the NHE's protein expression. Finally, intracellular Ca2+ concentration was detected by confocal laser scanning microscopy. The results demonstrated that the NHE6 mRNA and protein expressed in the human colon adenocarcinoma cell line (Caco-2). Furthermore, RV-Wa induced decreased expression of the NHE1 and NHE6 in Caco-2 cell in a time-dependent manner. In addition, intracellular Ca2+ concentration in RV-Wa-infected Caco-2 cells was higher than that in the mock-infected cells. Furthermore, RV-Wa also can downregulate the expression of calmodulin (CaM) and calmodulin kinase II (CaMKII) in Caco-2 cells. These findings provides important insights into the mechanisms of rotavirus-induced diarrhea. Further studies on the underlying pathophysiological mechanisms that downregulate NHEs in RV-induced diarrhea are required.


Asunto(s)
Regulación hacia Abajo , Regulación de la Expresión Génica/genética , Rotavirus/patogenicidad , Intercambiador 1 de Sodio-Hidrógeno/genética , Intercambiadores de Sodio-Hidrógeno/genética , Animales , Células CACO-2 , Calcio/análisis , Proteínas Quinasas Dependientes de Calcio-Calmodulina/metabolismo , Calmodulina/genética , Calmodulina/metabolismo , Línea Celular , Citosol/química , Diarrea/virología , Humanos , Macaca mulatta , ARN Mensajero/análisis , ARN Mensajero/biosíntesis , Infecciones por Rotavirus/genética , Infecciones por Rotavirus/virología , Intercambiador 1 de Sodio-Hidrógeno/metabolismo , Intercambiadores de Sodio-Hidrógeno/metabolismo , Transcripción Genética
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