Folding during sheeting improved qualities of dried noodles through gluten network proteins.

The texture properties after cooking for 12 min were selected to optimize the sheeting parameters, and the results were verified using the comprehensive quality of dried noodles. The distribution of water, characteristics of gluten protein, and interaction between gluten network and starch were analyzed to clarify the mechanism of the quality of dried noodles. Results showed that the optimal folding angle was 45°, under this condition, the largest anti-extension displacement perpendicular to the rolling direction and the smallest cooking loss were obtained. The hardness and smoothness of cooked noodles increased by about 14% to 17%. Further, the transverse relaxation time of strongly bound water significantly decreased, while the relative content and binding strength increased. The hydrogen bonds and α-helix contents increased by about 68.8% and 53.1%, respectively. Folding and sheeting enhanced the combination of starch granules and gluten network causing, decreased in the average length and porosity of the gluten network. It is depicted from the results that the method of optimizing the sheeting process based on the texture of dried noodles cooked for 12 min was feasible. And the 45° folding and sheeting could help to improve the quality of dried noodles.

Boosting Alkaline Seawater Oxidation of CoFe-layered Double Hydroxide Nanosheet Array by Cr Doping.

The pursuit of stable and efficient electrocatalysts toward seawater oxidation is of great interest, yet it poses considerable challenges. Herein, the utilization of Cr-doped CoFe-layered double hydroxide nanosheet array is reported on nickel-foam (Cr-CoFe-LDH/NF) as an efficient electrocatalyst for oxygen evolution reaction in alkaline seawater. The Cr-CoFe-LDH/NF catalyst can achieve current densities of 500 and 1000 mA cm -2 with remarkably low overpotentials of only 334 and 369 mV, respectively. Furthermore, it maintains at least 100 h stability when operated at 500 mA cm-2.

Copper-Catalyzed Three-Component Tandem Reaction of Alkynes, α-Diazo Esters, and TMSN3 to Access N-Substituted 1,2,3-Triazoles.

An efficient copper-catalyzed three-component tandem reaction of alkynes, α-diazo esters, and TMSN3 to construct triazoles has been developed. Through this strategy, a number of diverse N-substituted 1,2,3-triazoles were conveniently obtained in moderate to good yields from simple and readily available starting materials using K2CO3 as the base. The mechanism of the tandem Cu-catalyzed azide-alkyne cycloaddition (CuAAC) and Cu-carbenoid-participated C-N coupling reaction has been investigated.

Toxicological evaluation of TBBPA by common carp (Cyprinus carpio) about the in vivo/vitro disturbance of the AHR pathway.

Tetrabromobisphenol A (TBBPA) is a widely used plastic additive with high bioaccumulation potential and toxicity on both humans and wildlife. Currently, research on its ecotoxicity and the underlying mechanism is limited. Using common carp (Cyprinus carpio), we evaluated the toxicity of TBBPA, especially focusing on its alteration of a key metabolism-related pathway aryl hydrocarbon receptor (AHR), using in vivo/vitro assays and in silico simulation. The 96 h LC50 of TBBPA of common carp was 4.2 mg/L and belonged to the acute toxic level II. The bioaccumulation potential of TBBPA follows the role of liver > gill > brain and varies between 3- and 14-day exposure. On the AHR pathway respect, as expected, the metabolism-related cyp1a1 and cyp1b1 were upregulated in the liver and brain. Ahr2, the receptor, was also upregulated in the brain under TBBPA exposure. The alteration of gene expression was tissue-specific while the difference between 3- or 14-day exposure was minor. AHR inhibition assay indicated the 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD)-induced AHR transactivation can be inhibited by TBBPA suggesting it is not a potent agonist but a competitive antagonist. In silico analysis indicated TBBPA can be successfully docked into the binding cavity with similar poses but still have AHR-form-specific interactions. Molecular dynamics simulation proved TBBPA can be more flexible than the coplanar ligand TCDD, especially in ccaAHR1b with greater root-mean-square deviation (RMSD), of which TCDD-induced transactivation seemed not to be blocked by TBBPA. This research increased the understanding of TBBPA toxicity and alteration of the AHR pathway, and pointed out the need to perform additional toxicology evaluation of emerging contaminants, especially on non-model species.

Base-promoted one-pot three-component desulphurization cross-coupling access to 4-cyanoimidazole.

A novel, straightforward, and scalable base-mediated one-pot three-component desulphurization cross-coupling strategy is reported for the synthesis of 4-cyanoimidazole derivatives. Over 35 examples are provided and achieved yields exceeding 85%. Notably, the majority of these readily available compounds can be isolated through simple filtration, thereby circumventing the need for laborious column chromatography. Besides, the present protocol can be scaled up to 10 mmol with a yield of 87%, demonstrating promising potential for industrial applications.

Simultaneous and dynamic measurement of Schisandrol A changes in rat blood and brain and its comparative pharmacokinetic study in control and Parkinson's disease rats by dual-probe in vivo microdialysis.

Schisandrol A (SchA) is the main active ingredient of Schisandra chinensis (Turcz.) Baill., which is a famous traditional Chinese herbal medicine. SchA can penetrate the blood-brain barrier and has a significant neuroprotective effect. A group of multiplexed stable isotope mass tags (MSIMTs, m/z 332, 338, 346, 349, 351, 354, 360, 363, 374 and 377) were synthesized to perform multiplexed stable isotope labeling derivatization (MSILD) of SchA in rat microdialysates and standards. A new magnetic molecularly imprinted polymer was prepared using MSIMT-375-SchA as dummy template. All the 10-plexed derivatives of MSIMTs-SchA can be efficiently and selectively enriched and purified using this adsorbent by magnetic dispersive solid phase extraction (MDSPE) before ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) analysis. It should be pointed out that the MSIMT-346-SchA standard derivative was used as internal standard in the process of MDSPE and UHPLC-MS/MS. On these bases, 9 different rat microdialysate samples can be determined by UHPLC-MS/MS in a single run. The utilization of MSIMTs significantly increased the sensitivity, accuracy, selectivity and analysis throughput. Under the optimized conditions, satisfactory linearity (R2> 0.987), limit of detection (LODs, 0.15-0.26 pg/mL) and lower limit of quantitative (LLOQ, 0.8-2.0 pg/mL) were obtained. Intra- and inter-day precisions were in the range of 2.2% -12.5%, and recoveries 94.2% -106.2%. The matrix effects were very low, and the average derivatization efficiency of 10-plex MSIMTs to SchA was as high as 97.8%. Using the developed dual-probe in vivo microdialysis sampling technique, the proposed analytical method has been applied for comparative pharmacokinetics of SchA in the brain and blood of control and Parkinson's disease (PD) rats.

Ratiometric fluorescence assay for sulfide ions with fluorescent MOF-based nanozyme.

A novel ratiometric fluorescence strategy for sulfide ions (S2-) analysis has been developed using metal-organic framework (MOF)-based nanozyme. NH2-Cu-MOF displays blue fluorescence (λem = 435 nm) originating from 2-amino-1,4-benzenedicarboxylic acid ligand. Besides, it possesses oxidase-like activity due to Cu2+ node, which can trigger chromogenic reaction. o-Phenylenediamine (OPD), as a common enzyme substrate, can be oxidized by NH2-Cu-MOF to form luminescent products (oxOPD) (λem = 570 nm). Inner filter effect occurs between oxOPD and MOF. Upon exposure to S2-, oxidase-like activity of MOF is depressed significantly because of the generation of CuS. On one hand, the amount of free Cu2+ decreases, affecting the yielding of oxOPD. On the other hand, CuNPs with larger size are obtained during the oxidation-reduction reaction between Cu2+ and OPD, which show weaker autocatalytic ability for OPD oxidation. These result in the decrease and increase of intensities at 570 and 435 nm, respectively. This method exhibits sensitive and selective responses towards S2- with LOD of 0.1 µM. Furthermore, such ratiometric strategy has been applied to detect S2- in food samples.

Ratiometric fluorescence detection of artemisinin based on photoluminescent Zn-MOF combined with hemin as catalyst.

Artemisinin (ART) is a type of frontline drug to treat drug-resistant falciparum malaria. Simple, accurate and selective determination of ART is significant to monitor its clinical pharmaceutical efficacy. Herein, a new ratiometric fluorescence method has been designed for the determination of ART with Zn-MOF as fluorescence reference and hemin as catalyst, respectively. Zn-MOF possesses intrinsic fluorescence at 443 nm owing to 2-aminoterephthalic acid ligand. When o-phenylenediamine (OPD) is mixed with hemin, a weak fluorescent signal at 570 nm ascribed to oxidized product of OPD (oxOPD) is observed. In the presence of ART, hemin can catalyze ART to break its peroxide bridge and release a large number of reactive oxygen species, which effectively oxidize OPD into luminescent oxOPD. Therefore, the fluorescence at 570 nm is enhanced significantly while the fluorescence of Zn-MOF remains basically unchanged. Thus, a ratiometric fluorescence sensing platform has been constructed for the detection of ART. This method exhibits wider linear range (0.15 µM-150 µM) with detection limit of 50 nM. This novel and selective method has been used to detect ART in compound naphthoquinone phosphate tablets.

SYSTEMATIC SKILL PRACTICE IN WOMEN's FLOOR EXERCISE / Prática sistemática de habilidades no exercício de solo feminino / Práctica sistemática de habilidades en el ejercicio de suelo femenino

ABSTRACT Introduction Ground exercise is a high-level event of Chinese women's gymnastics. Maintaining the dominant strength of women's floor exercise in China has become one of the issues that coaches, athletes and gymnastics researchers should actively discuss. A research analysis on athletes' competition in the National Games can better reflect and illustrate the fundamental level of athletes and their overall developmental status. Objective To verify the relationship between women's floor exercise skills and competition performance. Methods The analysis and study of 65 female athletes participating in floor exercises at the National Games was carried out. In this paper, the on-the-spot statistical method is used to perform statistics on the skills and the athletes' completion in the four games. Movement training and choreography experts were consulted. Results The performance of women's floor drills in the National Games was discussed from three aspects: the difficulty and arrangement of movements, the initial score and bonus points, and the completion of movements. Conclusion Most athletes can adapt to the changes in the new rules and master many complex movements. This also gets them a higher score. However, the choreography of the athletes' complete movements is relatively simple and lacks features. Strength training in the lower limbs should be the key to improving the athletes' ability in floor exercise. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução O exercício no solo é um evento de alto nível da ginástica feminina chinesa. Manter a força dominante do exercício físico feminino na China tornou-se uma das questões que treinadores, atletas e pesquisadores de ginástica devem discutir ativamente. Uma análise de pesquisa sobre a competição dos atletas nos Jogos Nacionais pode refletir e ilustrar melhor o nível fundamental dos atletas e seu status geral de desenvolvimento. Objetivo Verificar a relação entre as habilidades de exercício do solo feminino e o desempenho na competição. Métodos Foi efetuada a análise e estudo de 65 atletas femininas que participam de exercícios de solo nos Jogos Nacionais. Neste trabalho, o método estatístico no local é utilizado para realizar estatísticas sobre as habilidades e a conclusão dos atletas nos quatro jogos. Foram consultados especialistas em treino de movimento e coreografia. Resultados Discutiu-se o desempenho dos exercícios de piso feminino nos Jogos Nacionais a partir de três aspectos: a dificuldade e a disposição dos movimentos, a pontuação inicial e os pontos de bônus, e a conclusão dos movimentos. Conclusão A maioria dos atletas pode se adaptar às mudanças nas novas regras e dominar muitos movimentos complexos. Isto também obtém uma pontuação mais alta. Entretanto, a coreografia dos movimentos completos dos atletas é relativamente simples e carece de características. O treinamento da força nos membros inferiores deve ser a chave para melhorar a habilidade das atletas no exercício do solo. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción El ejercicio en el suelo es una prueba de alto nivel de la gimnasia femenina china. Mantener la fuerza dominante del ejercicio de suelo femenino en China se ha convertido en una de las cuestiones que entrenadores, atletas e investigadores de la gimnasia deben debatir activamente. Un análisis de la investigación sobre la competición de los atletas en los Juegos Nacionales puede reflejar e ilustrar mejor el nivel fundamental de los atletas y su estado de desarrollo general. Objetivo Verificar la relación entre las habilidades del ejercicio de suelo de las mujeres y el rendimiento en la competición. Métodos Se llevó a cabo el análisis y estudio de 65 atletas femeninas que participaron en ejercicios de suelo en los Juegos Nacionales. En este trabajo se utiliza el método estadístico in situ para realizar estadísticas sobre las habilidades y la realización de los atletas en los cuatro partidos. Se consultó a expertos en formación del movimiento y coreografía. Resultados El rendimiento de los ejercicios de suelo de las mujeres en los Juegos Nacionales se analizó desde tres aspectos: la dificultad y la disposición de los movimientos, la puntuación inicial y los puntos de bonificación, y la finalización de los movimientos. Conclusión La mayoría de los atletas pueden adaptarse a los cambios de las nuevas reglas y dominar muchos movimientos complejos. Esto también obtiene una mayor puntuación. Sin embargo, la coreografía de los movimientos completos de los atletas es relativamente simple y carece de características. El entrenamiento de la fuerza en los miembros inferiores debe ser la clave para mejorar la capacidad de los atletas en el ejercicio de suelo. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Ratiometric fluorescence sensing of formaldehyde in food samples based on bifunctional MOF.

A new fluorescence strategy was described for ratiometric sensing of formaldehyde (FA) with bifunctional MOF, which acted as a fluorescence reporter as well as biomimetic peroxidase. With the assistance of H2O2, NH2-MIL-101 (Fe) catalyzes the oxidation of non-luminescent substrate o-phenylenediamine (OPD) to produce fluorescent product (oxOPD) with the maximum emission at 570 nm. Besides, intrinsic fluorescence of MOF (λem = 445 nm) was quenched by oxOPD through inner filter effect (IFE). However, FA and OPD reacted to generate Schiff bases, which competitively consumed OPD inhibiting the generation of oxOPD. Under the excitation wavelength of 375 nm, a ratiometric strategy was designed to detect FA with the fluorescence intensity ratio at 445 nm and 570 nm (F445/F570) as readout signal. This strategy exhibited a wide linear range (0.1-50 µM) and low detection limit of 0.03 µM. This method was confirmed for FA detection in food samples. In addition to establishing a new method to detect FA, this work will open new applications of MOF in food safety.

CoS2@TiO2 nanoarray: a heterostructured electrocatalyst for high-efficiency nitrate reduction to ammonia.

Electrochemical nitrate (NO3-) reduction emerges as a promising strategy to maintain the balance of the global nitrogen cycle and an alternative to nitrogen electroreduction for ambient ammonia (NH3) synthesis. However, the complicated multiple-electron transfer process of NO3--to-NH3 conversion demands catalysts with high selectivity for NH3 production. Herein, CoS2 nanoparticle decorated TiO2 nanobelt array on a titanium plate (CoS2@TiO2/TP) is reported as a superb electrocatalyst for the NO3- reduction reaction. In 0.1 M NaOH containing 0.1 M NO3-, CoS2@TiO2/TP offers a large NH3 yield of 538.21 µmol h-1 cm-2 at -0.7 V vs. reversible hydrogen electrode (RHE) and a high faradaic efficiency of 92.80% at -0.5 V vs. RHE. Additionally, it also shows strong stability for the 20 h electrolysis test.

Enhanced detection of ascorbic acid with cascaded fluorescence recovery of a dual-nanoquencher system.

An innovative strategy with target-triggered cascade fluorescence recovery of a dual-nanoquencher system was developed to detect ascorbic acid (AA). Herein, manganese dioxide (MnO2) nanosheets and gold nanoparticles (AuNPs) were used as nanoquenchers simultaneously. Owing to their synergistic effects, the fluorescence of 2,3-diaminophenazine (DAP) was decreased efficiently, thus minimizing the background fluorescence. The introduction of AA triggered the decomposition of MnO2 into Mn2+, which induced the aggregation of AuNPs. Both the decomposed MnO2 and aggregated AuNPs possess weak quenching abilities towards DAP. Such a cascade amplification strategy enhanced the detection sensitivity for AA with a LOD as low as 6.7 nM, which was two orders of magnitude lower than that of MnO2-based fluorescence assay. Furthermore, this amplification strategy was successfully applied to detect AA in food samples.

Cascade amplification strategy combined with analyte-triggered fluorescence switching of dual-quenching system for highly sensitive detection of isoniazide.

A sensitive fluorescence sensing platform consisting of manganese dioxide nanosheets (MnO2) and gold nanoparticles (AuNPs) as dual nanoquenchers has been constructed to detect isoniazid combined with analyte-triggered cascade reactions. The fluorescence of 2,3-diaminophenazine (DAP) is quenched simultaneously by MnO2 and AuNPs via inner filter effect. MnO2 is decomposed by isoniazid to generate Mn2+, which makes AuNPs aggregated. The quenching abilities of both the decomposed MnO2 and aggregated AuNPs are inhibited, causing remarkable fluorescence recovery. The usage of dual nanoquenchers enhances the quenching efficiency and reduces the fluorescence background. Moreover, the isoniazid-triggered cascade reaction further amplifies the readout signal. Thus, this strategy exhibits higher sensitivity towards the detection of isoniazid. Compared with MnO2-based fluorescence assay, this strategy possesses lower limit of detection. This strategy has been successfully used to detect isoniazid in pharmaceutical preparations, which is of great significance for drug analysis.

A T-rich nucleic acid-enhanced electrochemical platform based on electroactive silver nanoclusters for miRNA detection.

DNA-templated silver nanoclusters (DNA/AgNCs) serve as a useful electrochemical sensing nanomaterial characterized by excellent electroactivity and good stability, while the effect of surrounding nucleotides on their electroactivity has not been studied. Herein, we validated a nucleotide-assisted enhancement mechanism of the DNA/AgNCs electroactivity caused by T-rich nucleic acid sequences in the vicinity of AgNCs. Based on the T-rich nucleic acid-enhanced AgNCs (NAE-AgNCs) combined with hybrid chain reaction (HCR), a novel signal-enhanced electrochemical biosensing platform was established for the ultrasensitive detection of miRNA. In the presence of target miRNA-155, HCR could be triggered to generate duplex strands containing both numerous AgNC synthesis sites and T-rich overhang strands upon the electrode. With the electrodeposition of adjacent AgNCs on the electrode, the larger oxidation potential of T-rich nucleic acid leaded to stronger electron-accepting capacity, which could contribute to increased current responses. The T-rich NAE HCR electrochemical strategy resulted in a detection limit of 0.39 fM for miRNA-155 detection, one order of magnitude lower than conventional HCR-based electrochemical sensors. This T-rich nucleic acid-assisted enhancement mechanism provided a new direction to construct highly sensitive, label-free, low-cost, and simple sensing platforms for applications in biomarker assays and clinic diagnosis.

Comprehensive study on identification of water inrush sources from deep mining roadway.

To accurately identify the location of the water inrush sources, correlation analysis and factor analysis were used to discuss the similarity of hydrochemical control mechanism of different aquifers and inrush water. On this basis, Fisher and BP neural network are used to judge the probability of inrush water. Finally, it is verified by hydrological observation holes. According to the water sources mixing model, the water inrush sources of 11601 working face were mainly No. 13 stratum Carboniferous limestone water with a small amount of No. 10 stratum Carboniferous limestone water, and the mixing ratio is 11:1. Combining the results of water inrush source identification, water yield mutation, geophysical exploration results, and underground drilling, it is speculated that there are two faults in this working face. The normal fault stops at the top of No. 13 stratum Carboniferous limestone aquifer, and the reverse fault develops to No. 13 stratum Carboniferous limestone aquifer. This research is of great significance for the identification of mine water sources and guidance for the prevention and control of water inrush.

Reducing the sports injury through function movement biological image data screening / Reduzindo lesões esportivas por meio da triagem de dados biológicos de imagens de movimento / Reducción de las lesiones deportivas mediante la detección de datos biológicos por imágenes en movimiento

ABSTRACT Introduction Among the many cases of sports injuries, the incidence of musculoskeletal injuries remains high. After a musculoskeletal injury occurs, athletes often need to suspend training and undergo rehabilitation. A suitable sport mode requires athletes to have sufficient joint range of motion, core stability, and balance ability in different positions to complete various complex movements in training and competition. Objective The paper analyzes the impact of warm-up exercises before the test on the test results of functional sports biological image data screening ( FMS TM ) and provides references for unifying test conditions, checking the reliability of FMS TM repeated tests, and discussing the comparability of the research results. Methods The paper used the same password and process to test 12 young male volleyball professional athletes without warm-up and warm-up. The two-dimensional motion analysis system Dartfish Pursuit 8.0 was used to analyze the video data recorded by the two cameras simultaneously. Results In the two tests before and after, the hurdle step score (1.75±0.62 vs. 2.42±0.52) and the total score (13.50±2.20 vs. 16.42±2.15) were significantly higher than those without the warm-up test (P<0.01). Besides, in squats (1.58±0.67 vs. 1.92±0.67), straight lunges (2.00±0.43 vs. 2.50±0.52), active straight leg lifts (1.50±0.67 vs. 2.00±0.60), rotation stability (1.42) ±0.52 vs. 1.92±0.29) showed a significant increase in the score (P<0.05). Conclusions Warm-up exercises before the test can improve the test results. This abnormal movement pattern observed only by visual inspection may not truly reflect the "dysfunction" of the movement. In the case of ignoring the pre-test warm-up factors, feedback on sports performance and formulating training strategies, this conclusion of predicting injury risk and evaluating training effects may have specific limitations in its reference value. Level of evidence II; Therapeutic studies - investigation of treatment results.

RESUMO Introdução Dentre os diversos casos de lesões esportivas, a incidência de lesões musculoesqueléticas permanece elevada. Após a ocorrência de uma lesão musculoesquelética, os atletas geralmente precisam suspender o treinamento e se submeter à reabilitação. Um modo de esporte adequado requer que os atletas tenham amplitude de movimento articular suficiente, estabilidade central e capacidade de equilíbrio em diferentes posições para completar vários movimentos complexos em treinamento e competição. Objetivo o artigo analisa o impacto dos exercícios de aquecimento antes do teste sobre os resultados dos testes de triagem de dados de imagens biológicas esportivas funcionais (FMSTM) e fornece referências para unificar as condições de teste, verificar a confiabilidade de testes FMSTM repetidos e discutir a comparabilidade dos resultados da pesquisa . Métodos O artigo utilizou a mesma senha e processo para testar 12 jovens atletas profissionais do sexo masculino de voleibol sem aquecimento e com aquecimento. O sistema de análise de movimento bidimensional Dartfish Pursuit 8.0 foi usado para analisar os dados de vídeo gravados pelas duas câmeras simultaneamente. Resultados Nos dois testes antes e depois, a pontuação do hurdle step (1,75 ± 0,62 vs. 2,42 ± 0,52) e a pontuação total (13,50 ± 2,20 vs. 16,42 ± 2,15) foram significativamente maiores do que aqueles sem o teste de aquecimento ( P <0,01). Além disso, em agachamentos (1,58 ± 0,67 vs. 1,92 ± 0,67), estocadas retas (2,00 ± 0,43 vs. 2,50 ± 0,52), levantamentos ativos de perna reta (1,50 ± 0,67 vs. 2,00 ± 0,60), estabilidade de rotação (1,42) ± 0,52 vs. 1,92 ± 0,29) mostrou um aumento significativo na pontuação (P <0,05). Conclusão Os exercícios de aquecimento antes do teste podem melhorar os resultados do teste. Este padrão de movimento anormal observado apenas por inspeção visual pode não refletir verdadeiramente a "disfunção" do movimento. No caso de ignorar os fatores de aquecimento pré-teste, feedback sobre o desempenho esportivo e formulação de estratégias de treinamento, esta conclusão de prever o risco de lesões e avaliar os efeitos do treinamento pode ter limitações específicas em seu valor de referência. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción Entre los muchos casos de lesiones deportivas, la incidencia de lesiones musculoesqueléticas sigue siendo alta. Después de que ocurre una lesión musculoesquelética, los atletas a menudo necesitan suspender el entrenamiento y someterse a rehabilitación. Un modo de deporte adecuado requiere que los atletas tengan suficiente rango de movimiento articular, estabilidad central y capacidad de equilibrio en diferentes posiciones para completar varios movimientos complejos en el entrenamiento y la competencia. Objetivo El documento analiza el impacto de los ejercicios de calentamiento antes de la prueba en los resultados de las pruebas de detección de datos de imágenes biológicas deportivas funcionales (FMSTM) y proporciona referencias para unificar las condiciones de prueba, verificar la confiabilidad de las pruebas FMSTM repetidas y discutir la comparabilidad de los resultados de la investigación. Métodos El documento utilizó la misma contraseña y proceso para evaluar a 12 jóvenes atletas profesionales de voleibol sin calentamiento y con calentamiento. El sistema de análisis de movimiento bidimensional Dartfish Pursuit 8.0 se utilizó para analizar los datos de video grabados por las dos cámaras simultáneamente. Resultados En las dos pruebas antes y después, la puntuación del paso de valla (1,75 ± 0,62 frente a 2,42 ± 0,52) y la puntuación total (13,50 ± 2,20 frente a 16,42 ± 2,15) fueron significativamente más altas que aquellas sin la prueba de calentamiento ( P <0,01). Además, en sentadillas (1,58 ± 0,67 vs 1,92 ± 0,67), estocadas rectas (2,00 ± 0,43 vs 2,50 ± 0,52), levantamientos activos de piernas rectas (1,50 ± 0,67 vs 2,00 ± 0,60), estabilidad de rotación (1,42) ± 0,52 vs. 1,92 ± 0,29) mostró un aumento significativo en la puntuación (P <0,05). Conclusión Los ejercicios de calentamiento antes de la prueba pueden mejorar los resultados de la prueba. Este patrón de movimiento anormal observado solo por inspección visual puede no reflejar realmente la "disfunción" del movimiento. En el caso de ignorar los factores de calentamiento previos a la prueba, la retroalimentación sobre el rendimiento deportivo y la formulación de estrategias de entrenamiento, esta conclusión de predecir un riesgo de lesión y evaluar los efectos del entrenamiento puede tener limitaciones específicas en su valor de referencia. Nivel de evidencia II; Estudios terapéuticos: investigación de los resultados del tratamiento.

[Recent advances in chemical derivatization-based chromatography-mass spectrometry methods for analysis of aldehyde biomarkers].

Human exposure to chemical pollutants in the environment can cause a variety of diseases, including cancer, diabetes, cardiovascular disease, and neurodegenerative diseases (Alzheimer's disease, Parkinson's disease, etc.). Exogenous and environmental pollutant exposure-induced endogenous aldehydes are highly reactive electrophilic compounds that can form covalently modified products with a variety of important biological molecules in the human body, thus inducing toxic effects. Exposome research has become a hotspot since it was first proposed in 2005. Exposure studies can map the complex relationships between biomarkers and disease risk. Therefore, the measurable and characteristic changes of all biomarkers together constitute a key basis for exposome research. Aldehydes are among the main components of chemical exposure. Because of the physical and chemical properties of aldehydes and the existence of multiple matrix interferences in the samples, it is particularly difficult to analyze and characterize them. The analysis and detection methods for aldehydes mainly include sensing analysis, electrochemical methods, fluorescence imaging, chromatography, mass spectrometry (MS), and chromatography-MS. Analytical techniques based on gas chromatography-MS (GC-MS) and liquid chromatography-MS (LC-MS) have emerged as the main methods for chemical exposome research. Chemical derivatization, especially stable isotope labeling derivatization (also known as chemical isotope labeling) combined with LC-MS analytical techniques, can help circumvent the problems encountered in targeted and non-targeted metabolome and exposome analysis. The combination of chemical derivatization with chromatography-MS is one of the most important solutions for the accurate analysis of aldehydes in complex samples. Over the past five years, the development and application of chromatography-MS analytical methods based on chemical derivatization have become key topics in aldehyde analysis. This paper summarizes and reviews the latest progress in GC-MS and LC-MS methods based on chemical derivatization (2015-2020). The review focuses on analytical method development for aldehyde exposure biomarkers in bio-matrices (blood, urine, saliva, biological tissue, etc.). Various derivatization reagents for labeling small-molecule aldehydes, qualitative/quantitative analytical methods and their application value, advantages/disadvantages of different analytical methods for aldehyde exposure biomarkers, and future development trends are also included. The manuscript contents may aid the integrated development of exposome, metabolomics, and lipidomics, as well as research on the environment, ecology, and health. To clarify the complex actions of exogenous and endogenous aldehydes in physiological and pathological events, it is necessary to improve the analysis and characterization techniques and tools for studying the "aldehydome." With the development and application of sophisticated mass spectrometers, advances in high-performance chromatographic separation and bioinformatics, and advent of single-cell analysis and MS imaging, future aldehyde exposome analytical methods will have higher sensitivity and throughput. This in turn would be more useful for screening and identifying unknown aldehyde compounds and discovering new exposome biomarkers.

Fluorescent DNA-templated silver nanoclusters for highly sensitive detection of D-penicillamine.

Herein, fluorescent DNA-templated silver nanoclusters (DNA-AgNCs) with red emission were synthesized and utilized as novel probe to detect D-penicillamine (D-Pen) for the first time. D-Pen molecules contain a thiol which can combine with Ag to form a non-fluorescent ground state complex, inducing the aggregation of DNA-AgNCs followed by the fluorescence quenching. The quenching mechanism is well-studied and found to be a static quenching process. This method can detect D-Pen in the range of 0.025-0.7 µM with the detection limit as low as 8 nM, which is 1-3 orders of magnitude more sensitive than those based on other fluorescent nanoprobes. More importantly, the preparation procedure for DNA-AgNCs is fast and without the requirement of heavy metal ions. Thus, this detection strategy is time-saving and eco-friendly. Satisfactory recoveries have been acquired for monitoring D-Pen in human serum samples and pharmaceutical samples owing to the high sensitivity.

A ratiometric fluorescence assay for bleomycin based on dual-emissive chameleon DNA-templated silver nanoclusters.

The authors design dual-emissive DNA-templated silver nanoclusters (DNA-AgNCs) for ratiometric fluorescence sensing bleomycin (BLM) for the first time. A hairpin probe containing two different C-rich DNA templates at two terminals is used to synthesize chameleon DNA-AgNCs, which possess two emission peaks when they are in close proximity. A strong emission is founded at 622 nm (λex = 570 nm) while a weak one is located at 572 nm (λex = 504 nm). Meanwhile, the loop of this probe contains the scission site (5'-GC-3') of BLM. The loop can be cleaved into two parts by BLM-Fe(II) complex, inducing the two DNA-AgNCs away from each other. The fluorescence intensity at 572 nm and 622 nm increases and decreases, respectively. Such chameleon DNA-AgNCs exhibit an obvious fluorescence discoloration from orange to yellow. Therefore, a sensitive ratiometric fluorescent strategy for BLM detection has been proposed with the detection limit of 67 pM. Finally, this ratiometric method is used to detect BLM in serum samples.

Analysis of hydrochemical evolution in main discharge aquifers under mining disturbance and water source identification.

To discuss the hydrochemical evolution characteristics of the mining process of Peigou Coal Mine, based on the test results of 43 water samples collected at different times from three main discharge aquifers, namely, Carboniferous Taiyuan Formation limestone water (L7-8 + L5-6 water), Ordovician limestone water (including Taiyuan Formation L1-4), and Permian main mining coal seam roof and floor sandstone water (roof and floor water), a hydrochemical evolution model of the mining disturbances since 2003 has been established. The carbonate and sulphate dissolution and pyrite oxidation in Ordovician limestone water significantly decreased and then increased in 2006, and silicate weathering was weak. The carbonate and sulphate dissolution, silicate weathering and pyrite oxidation of roof and floor sandstone water increased. At the same time, a water source identification model suitable for the Peigou Coal Mine was developed by comparing the Fisher discriminant and the BP (back propagation) neural network discriminant. The accuracy rates of Fisher discriminant and BP neural network discriminant are 81.40% and 83.72% respectively, which indicates that BP neural network is more accurate. Finally, the evolution of hydraulic connection between aquifers is analysed. We speculate that there is a fracture development channel between Ordovician limestone water and roof and floor water aquifers that is affected in 2005 by the mining disturbance. This study has significance for examining the hydrochemical evolution of groundwater in mines and acting as a guideline to prevent and control water inrushes.