Microbial functional heterogeneity induced in a petroleum-polluted soil profile.

Microbial taxonomic diversity declines with increasing stress caused by petroleum pollution. However, few studies have tested whether functional diversities vary similarly to taxonomic diversity along the stress gradient. Here, we investigated soil microbial communities in a petrochemically polluted site in China. Total petroleum hydrocarbon (TPH) concentrations were higher in the middle (2-3 m) and deep soil layer (3-5 m) than in the surface soil layer (0-2 m). Accordingly, microbial taxonomic α-diversity was decreased by 44% (p < 0.001) in the middle and deep soil layers, compared to the surface soil layer. In contrast, functional α-diversity decreased by 3% (p < 0.001), showing a much better buffering capacity to environmental stress. Differences in microbial taxonomic and functional ß-diversities were enlarged in the middle and deep soil layers, extending the Anna Karenina Principle (AKP) that a community adapts to stressful environments in its own way. Consistent with the stress gradient hypothesis, we revealed a higher degree of network connectivity among microbial species and genes in the middle and deep soil layers compared to the surface soil layer. Together, we demonstrate that microbial functionality is more tolerant to stress than taxonomy, both of which were amenable to AKP and the stress gradient hypothesis.

Petroleum pollution changes microbial diversity and network complexity of soil profile in an oil refinery.

Introduction: Petroleum pollution resulting from spills and leakages in oil refinery areas has been a significant environmental concern for decades. Despite this, the effects of petroleum pollutants on soil microbial communities and their potential for pollutant biodegradation still required further investigation. Methods: In this study, we collected 75 soil samples from 0 to 5 m depths of 15 soil profiles in an abandoned refinery to analyze the effect of petroleum pollution on soil microbial diversity, community structure, and network co-occurrence patterns. Results: Our results suggested soil microbial a-diversity decreased under high C10-C40 levels, coupled with significant changes in the community structure of soil profiles. However, soil microbial network complexity increased with petroleum pollution levels, suggesting more complex microbial potential interactions. A module specific for methane and methyl oxidation was also found under high C10-C40 levels of the soil profile, indicating stronger methanotrophic and methylotrophic metabolic activities at the heavily polluted soil profile. Discussion: The increased network complexity observed may be due to more metabolic pathways and processes, as well as increased microbial interactions during these processes. These findings highlight the importance of considering both microbial diversity and network complexity in assessing the effects of petroleum pollution on soil ecosystems.

Degradation of sulfadimethoxine by permanganate in aquatic environment: Influence factors, intermediate products and theoretical study.

The excess sulfadimethoxine (SDM) in the environment could lead to antibiotic resistance by microorganisms and may do harm to many aquatic organisms. In this work, the removal of SDM by potassium permanganate (KMnO4) was comprehensively studied. The influence of various factors, including the pH, oxidant doses, and temperature, on SDM removal were investigated. The optimal reaction conditions were determined to be pH 5.0, T = 25 °C and [KMnO4]0 = 200 µmol L-1. Anions (Cl-, SO42-, HCO32-, and NO3-) and cations (K+, Ca2+, Mg2+, and NH4+) had no significant influence on the removal of SDM. However, H2PO4- improved the efficiency of SDM removal by KMnO4. Humic acid (0-10 mg L-1) promoted the removal of SDM, which was attributed to the generation of in situ MnO2. Meanwhile, the degradation of SDM in various water matrices was studied, and the removal order was ultrapure water > Jiuxiang river water ≈ synthetic water > secondary clarifier effluent. According to ten intermediate products identified and a frontier electron densities (FED) calculation, several pathways were proposed that involve the oxidation of amidogen, the cleavage of CS and SN bonds, and an oligomerization reaction. The predicted toxicity assessment indicated that most of the degradation products were not harmful to aquatic organisms except SDM dimers (connection by HNNH), suggesting that byproducts, such as dimers, formed during the oxidation of SDM and other sulfonamides should be taken into consideration. In sum, KMnO4 has the potential to remove SDM from the aquatic environment.