Comparison of decontamination efficacy of two electrolyte cleaning methods to diode laser, plasma, and air-abrasive devices.

OBJECTIVE: To compare the in vitro decontamination efficacy of two electrolytic cleaning methods to diode laser, plasma, and air-abrasive devices. MATERIAL AND METHODS: Sixty sandblasted large-grit acid-etched (SLA) implants were incubated with 2 ml of human saliva and Tryptic Soy Broth solution under continuous shaking for 14 days. Implants were then randomly assigned to one untreated control group (n = 10) and 5 different decontamination modalities: air-abrasive powder (n = 10), diode laser (n = 10), plasma cleaning (n = 10), and two electrolytic test protocols using either potassium iodide (KI) (n = 10) or sodium formate (CHNaO2) (n = 10) solution. Implants were stained for dead and alive bacteria in two standardized measurement areas, observed at fluorescent microscope, and analyzed for color intensity. RESULTS: All disinfecting treatment modalities significantly reduced the stained area compared to the untreated control group for both measurement areas (p < 0.001). Among test interventions, electrolytic KI and CHNaO2 treatments were equally effective, and each one significantly reduced the stained area compared to any other treatment modality (p < 0.001). Efficacy of electrolytic protocols was not affected by the angulation of examined surfaces [surface angulation 0° vs. 60° (staining %): electrolytic cleaning-KI 0.03 ± 0.04 vs. 0.09 ± 0.10; electrolytic cleaning-CHNaO2 0.01 ± 0.01 vs. 0.06 ± 0.08; (p > 0.05)], while air abrasion [surface angulation 0° vs. 60° (staining %): 2.66 ± 0.83 vs. 42.12 ± 3.46 (p < 0.001)] and plasma cleaning [surface angulation 0° vs. 60° (staining %): 33.25 ± 3.01 vs. 39.16 ± 3.15 (p < 0.001)] were. CONCLUSIONS: Within the limitations of the present in vitro study, electrolytic decontamination with KI and CHNaO2 was significantly more effective in reducing bacterial stained surface of rough titanium implants than air-abrasive powder, diode laser, and plasma cleaning, regardless of the accessibility of the contaminated implant location. CLINICAL RELEVANCE: Complete bacterial elimination (residual bacteria < 1%) was achieved only for the electrolytic cleaning approaches, irrespectively of the favorable or unfavorable access to implant surface.

Correction: Ratka et al. The Effect of In Vitro Electrolytic Cleaning on Biofilm-Contaminated Implant Surfaces. J. Clin. Med. 2019, 8, 1397.

There was an error in the original article [...].

Influence of Anodizing Stages on the Preload Force of Implant-Abutment Screws and Their Benefits Regarding the Concept of Immediate Implant Placement-An In Vitro Study.

The tightening torque applied to a screw in a provisional restoration immediately after implant placement in a fresh extraction socket is often too low to gain sufficient preload force. Therefore, abutment screw loosening is a common complication. The aim of this study was to investigate whether it is possible to increase the preload force of a given tightening torque by anodizing parts of the implant-abutment complex. In test group 1 (TG1), only the abutment screw was anodized, in four different stages, whereas in test group 2 (TG2), the abutment and the threaded sleeve were anodized in four anodizing stages (TG2a-TG2d). The control group (CG) consisted of non-anodized components. The results were tested for normal distribution, and the components were subsequently parametrically analyzed using a linear model. Both test groups showed higher preload forces compared to the non-anodized control group. The CG obtained an average preload force of 390 N at a tightening torque of 35 Ncm. Comparable values were already obtained at a tightening torque of 20 to 30 Ncm in TG1c/D and TG2b/d. It can be concluded that anodization of abutment screws and components is an effective measure to increase the preload force of the abutment screws by a given tightening torque.

Influence of different agents on the preload force of implant abutment screws.

STATEMENT OF PROBLEM: Screw loosening is a common problem in implant dentistry; however, information is sparse on the influence of different fluids on the screw threads. PURPOSE: The purpose of this in vitro study was to investigate the influence of 4 different fluids and agents (saliva, blood, chlorhexidine [CHX] gel, and special sealing silicone) on the preload force of abutment screws. MATERIALS AND METHODS: The test specimens (N=50) consisted of a thread sleeve resembling the implant, an abutment analog, and an abutment screw. The tightening of the screw with a torque wrench was performed in 5 steps (15 Ncm, 20 Ncm, 25 Ncm, 30 Ncm, and 35 Ncm). Each agent was applied in the lumen of the thread sleeve of 10 specimens. Ten dry thread sleeves served as the control. Comparisons between 2 independent groups were performed with the t test or Wilcoxon-Mann-Whitney test, as appropriate. The Bonferroni correction was used for multiple comparisons (α=.05). RESULTS: Preload forces increased linearly with the applied tightening torque for dry implant lumina, as well as for saliva, blood, silicone, and CHX gel in the implant lumina or thread sleeves. In general, none of the tested agents resulted in significantly higher preload forces compared with the dry control. CONCLUSIONS: The agents investigated did not have any lubricant action on implant abutment screws.

Is Complete Re-Osseointegration of an Infected Dental Implant Possible? Histologic Results of a Dog Study: A Short Communication.

Complete reosseointegration after treatment of periimplantitis was never published yet. This short scientific communication reports about results of a randomized controlled preclinical study. An electrolytic approach was compared to a classical modality (ablative, cotton pellets soaked with sodium chloride solution and H2O2. For electrolytic cleaning a complete reosseointegration was achieved in several cases serving as a proof of concept.

Treatment of Peri-implantitis-Electrolytic Cleaning Versus Mechanical and Electrolytic Cleaning-A Randomized Controlled Clinical Trial-Six-Month Results.

OBJECTIVES: The present randomized clinical trial assesses the six-month outcomes following surgical regenerative therapy of periimplantitis lesions using either an electrolytic method (EC) to remove biofilms or a combination of powder spray and electrolytic method (PEC). MATERIALS AND METHODS: 24 patients with 24 implants suffering from peri-implantitis with any type of bone defect were randomly treated by EC or PEC. Bone defects were augmented with a mixture of natural bone mineral and autogenous bone and left for submerged healing. The distance from implant shoulder to bone was assessed at six defined points at baseline (T0) and after six months at uncovering surgery (T1) by periodontal probe and standardized x-rays. RESULTS: One implant had to be removed at T1 because of reinfection and other obstacles. None of the other implants showed signs of inflammation. Bone gain was 2.71 ± 1.70 mm for EC and 2.81 ± 2.15 mm for PEC. No statistically significant difference between EC and PEC was detected. Significant clinical bone fill was observed for all 24 implants. Complete regeneration of bone was achieved in 12 implants. Defect morphology impacted the amount of regeneration. CONCLUSION: EC needs no further mechanical cleaning by powder spray. Complete re-osseointegration in peri-implantitis cases is possible.

Heat Generation at the Implant-Bone Interface by Insertion of Ceramic and Titanium Implants.

PURPOSE: The aim of this study is to record material- and surface-dependent heat dissipation during the process of inserting implants into native animal bone. MATERIALS AND METHODS: Implants made of titanium and zirconium that were identical in macrodesign were inserted under controlled conditions into a bovine rib tempered to 37 °C. The resulting surface temperature was measured on two bone windows by an infrared camera. The results of the six experimental groups, ceramic machined (1), sandblasted (2), and sandblasted and acid-etched surfaces (3) versus titanium implants with the corresponding surfaces (4, 5, and 6) were statistically tested. RESULTS: The average temperature increase, 3 mm subcrestally at ceramic implants, differed with high statistical significance (p = 7.163 × 10-9, resulting from group-adjusted linear mixed-effects model) from titanium. The surface texture of ceramic implants shows a statistical difference between group 3 (15.44 ± 3.63 °C) and group 1 (19.94 ± 3.28 °C) or group 2 (19.39 ± 5.73 °C) surfaces. Within the titanium implants, the temperature changes were similar for all surfaces. CONCLUSION: Within the limits of an in vitro study, the high temperature rises at ceramic versus titanium implants should be limited by a very slow insertion velocity.

The Effect of In Vitro Electrolytic Cleaning on Biofilm-Contaminated Implant Surfaces.

PURPOSE: Bacterial biofilms are a major problem in the treatment of infected dental and orthopedic implants. The purpose of this study is to investigate the cleaning effect of an electrolytic approach (EC) compared to a powder-spray system (PSS) on titanium surfaces. MATERIALS AND METHODS: The tested implants (different surfaces and alloys) were collated into six groups and treated ether with EC or PSS. After a mature biofilm was established, the implants were treated, immersed in a nutritional solution, and streaked on Columbia agar. Colony-forming units (CFUs) were counted after breeding and testing (EC), and control (PSS) groups were compared using a paired sample t-test. RESULTS: No bacterial growth was observed in the EC groups. After thinning to 1:1,000,000, 258.1 ± 19.9 (group 2), 264.4 ± 36.5 (group 4), and 245.3 ± 40.7 (group 6) CFUs could be counted in the PSS groups. The difference between the electrolytic approach (test groups 1, 3, and 5) and PSS (control groups 2, 4, and 6) was statistically extremely significant (p-value < 2.2 × 10-16). CONCLUSION: Only EC inactivated the bacterial biofilm, and PSS left reproducible bacteria behind. Within the limits of this in vitro test, clinical relevance could be demonstrated.

The micromechanical behavior of implant-abutment connections under a dynamic load protocol.

BACKGROUND: The implant-abutment connection (IAC) is known to be a key factor for the long-term stability of peri-implant tissue. PURPOSE: The aim of the present in vitro study was to detect and measure the mechanical behavior of different IACs by X-ray imaging. MATERIALS AND METHODS: A total of 20 different implant systems with various implant dimensions and IACs (13 conical-, 6 flat-, and 1 gable-like IAC) have been tested using a chewing device simulating dynamic and static loading up to 200 N. Micromovements have been recorded with a high-resolution, high-speed X-ray camera, and gap length and gap width between implant and abutment have been calculated. Furthermore, X-ray video sequences have been recorded to investigate the sealing capacity of different IACs. RESULTS: Out of the 20 implant systems, eight implant systems with a conical IAC showed no measurable gaps under static and dynamic loading (200 N). By contrast, all investigated implant systems with a flat IAC showed measurable gaps under dynamic and static loading. X-ray video sequences revealed that a representative conical IAC had sufficient sealing capacity. CONCLUSION: Within the limits of the present in vitro study, X-ray imaging showed reduced formation of microgaps and consecutive micromovements in implants with conical IAC compared to flat IACs.

Effects of Screw Configuration on the Preload Force of Implant-Abutment Screws.

PURPOSE: The aim of this study was to investigate the effects of tightening torque, screw head angle, and thread number on the preload force of abutment screws. MATERIALS AND METHODS: The test specimens consisted of three self-manufactured components (ie, a thread sleeve serving as an implant analog, an abutment analog, and an abutment screw). The abutment screws were fabricated with metric M1.6 external threads. The thread number varied between one and seven threads. The screw head angles were produced in eight varying angles (30 to 180 degrees). A sensor unit simultaneously measured the preload force of the screw and the torsion moment inside the screw shank. The tightening of the screw with the torque wrench was performed in five steps (15 to 35 Ncm). The torque wrench was calibrated before each step. RESULTS: Only the tightening torque and screw head angle affected the resulting preload force of the implant-abutment connection. The thread number had no effect. There was an approximately linear correlation between tightening torque and preload force. CONCLUSION: The tightening torque and screw head angle were the only study parameters that affected the resulting preload force of the abutment screw. The results obtained from this experiment are valid only for a single torque condition. Further investigations are needed that analyze other parameters that affect preload force. Once these parameters are known, it will add value for a strong, but detachable connection between the implant and abutment. Short implants and flat-to-flat connections especially will benefit significantly from this knowledge.

A New Experimental Design for Bacterial Microleakage Investigation at the Implant-Abutment Interface: An In Vitro Study.

PURPOSE: This study aimed to test bacterial microleakage at the implant-abutment interface (IAI) before and after dynamic loading using a new chewing simulation. MATERIALS AND METHODS: Fourteen implant systems (n = 5 samples of each) were divided into two groups: (1) systems with conical implant-abutment connections (IACs), and (2) systems with flat IACs. For collecting samples without abutment disconnection, channels (Ø = 0.3 mm) were drilled into implants perpendicularly to their axes, and stainless-steel cannulas were adhesively glued inside these channels to allow a sterilized rinsing solution to enter the implant interior and to exit with potential contaminants for testing. Implants were embedded in epoxy resin matrices, which were supported by titanium cylinders with lateral openings for inward and outward cannulas. Abutments were tightened and then provided with vertically adjustable, threaded titanium balls, which were cemented using composite cement. Specimens were immersed in a bacterial liquid and after a contact time of 15 minutes, the implant interior was rinsed prior to chewing simulation (0 N ≘ static seal testing). Specimens were exposed to a Frankfurt chewing simulator. Two hundred twenty force cycles per power level (110 in ± X-axis) were applied to simulate a daily masticatory load of 660 chewing cycles (equivalent to 1,200,000 cycles/5 years). The applied load was gradually increased from 0 N to a maximum load of 200 N in 25-N increments. The implant interior was rinsed to obtain samples before each new power level. All samples were tested using fluorescence microscopy; invading microorganisms could be counted and evaluated. RESULTS: No bacterial contamination was detected under static loading conditions in both groups. After loading, bacterial contamination was detected in one sample from one specimen in group 1 and in two samples from two specimens in group 2. CONCLUSION: Controlled dynamic loading applied in this study simulated a clinical situation and enabled time-dependent analysis regarding the bacterial seal of different implant systems. Conical IACs offer a better bacterial seal compared with flat IACs, which showed increased microleakage after dynamic loading. IAC design plays a crucial role in terms of bacterial colonization. Taking samples of the implant interior without abutment disconnection eliminates an error source.

Microcomputed tomography-based assessment of retrieved dental implants.

PURPOSE: The aim of this study was to demonstrate the potential of microcomputed tomography (micro-CT) technology in the assessment of retrieved dental implants. Cases are presented to illustrate the value of micro-CT imaging techniques in determining possible mechanical causes for dental implant failures. MATERIALS AND METHODS: Eight retrieved dental implants were randomly selected from a pool and imaged using a micro-CT device. Source voltages (80 to 100 kV) and source-to-detector distances (65 to 70 mm) were based on signal quality requirements with an additional criterion of achieving the highest resolution with the sample entirely in the field of view in the projection plane. One additional sample was chosen for histology and tomographic imaging so that the information contained therein could be compared. RESULTS: The micro-CT images displayed high contrast between the implant, bone, and background, with negligible metal artifacts. The micro-CT technology used in this study delivered excellent images of the retrieved implants. As a result of the quality and resolution (pixel size: 5.52 to 6.15 µm) of the images, surface morphology as well as internal structures of the retrieved implants could be observed in great detail. The majority of the retrieved implants had increased wear, dents, pits, regular shallow scratches, and deep scratches in the implant-to-abutment engagement area. Furthermore, plastic deformations, microcracks, and brittle implant fractures were observed in two implants. CONCLUSION: The mechanical competence of dental implant components plays a major role in the success of implant treatment. When failures do occur, a nondestructive three-dimensional assessment of such failed implants and their components is helpful in understanding the underlying factors. Micro-CT was found to be a useful tool for the morphologic assessment of retrieved dental implants.

Reliable coupling of orthodontic elements to mini-implants: An in-vitro study.

OBJECTIVES: To investigate the reliability of mechanical and adhesive methods of fixing rectangular wires in the cross-slot of a mini-implant. MATERIALS AND METHODS: A twin-hooked wire element was placed and fixed in the slot of a mini-implant via a NiTi spring under tension, or by means of an adhesive. For the purpose of mechanical anchorage, the wire was crimped with a special crimping tool to increase its thickness and prevent it from slipping through the slot. Before applying the adhesive, there were four possible methods of preparation: Untreated Wire (Adh. 1); ROCATEC-Pre (Adh. 2); ROCATEC-PRE + Espe Sil (Adh. 3); ROCATEC-PRE + ROCATEC-PLUS + Espe Sil (Adh. 4). The mechanical fixing and two adhesive fixings were aged by means of temperature change (500 cycles). A Zwick universal testing machine was used to measure the maximum strength of the coupling. RESULTS: In all tests, the untreated wire + adhesive withstood the lowest maximum load (Ø 3 N and 10.8 N respectively) and failed the aging test after a maximum of eight cycles. The wires in test group Adh. 4 withstood the highest maximum load (Ø 43.3 N; 41.5 N; 45.9 N after aging) in all tests. The average load withstood by the crimped ligature was 38.7 N. CONCLUSION: The adhesive method of fixation performs best when the rectangular wire is sand blasted and silanized before application. The mechanical coupling using the crimped ligature is reliable.

Osseointegration of zirconia implants compared with titanium: an in vivo study.

BACKGROUND: Titanium and titanium alloys are widely used for fabrication of dental implants. Since the material composition and the surface topography of a biomaterial play a fundamental role in osseointegration, various chemical and physical surface modifications have been developed to improve osseous healing. Zirconia-based implants were introduced into dental implantology as an alternative to titanium implants. Zirconia seems to be a suitable implant material because of its tooth-like colour, its mechanical properties and its biocompatibility. As the osseointegration of zirconia implants has not been extensively investigated, the aim of this study was to compare the osseous healing of zirconia implants with titanium implants which have a roughened surface but otherwise similar implant geometries. METHODS: Forty-eight zirconia and titanium implants were introduced into the tibia of 12 minipigs. After 1, 4 or 12 weeks, animals were sacrificed and specimens containing the implants were examined in terms of histological and ultrastructural techniques. RESULTS: Histological results showed direct bone contact on the zirconia and titanium surfaces. Bone implant contact as measured by histomorphometry was slightly better on titanium than on zirconia surfaces. However, a statistically significant difference between the two groups was not observed. CONCLUSION: The results demonstrated that zirconia implants with modified surfaces result in an osseointegration which is comparable with that of titanium implants.

Behavior of osteoblastic cells cultured on titanium and structured zirconia surfaces.

BACKGROUND: Osseointegration is crucial for the long-term success of dental implants and depends on the tissue reaction at the tissue-implant interface. Mechanical properties and biocompatibility make zirconia a suitable material for dental implants, although surface processings are still problematic. The aim of the present study was to compare osteoblast behavior on structured zirconia and titanium surfaces under standardized conditions. METHODS: The surface characteristics were determined by scanning electron microscopy (SEM). In primary bovine osteoblasts attachment kinetics, proliferation rate and synthesis of bone-associated proteins were tested on different surfaces. RESULTS: The results demonstrated that the proliferation rate of cells was significantly higher on zirconia surfaces than on titanium surfaces (p < 0.05; Student's t-test). In contrast, attachment and adhesion strength of the primary cells was significant higher on titanium surfaces (p < 0.05; U test). No significant differences were found in the synthesis of bone-specific proteins. Ultrastructural analysis revealed phenotypic features of osteoblast-like cells on both zirconia and titanium surfaces. CONCLUSION: The study demonstrates distinct effects of the surface composition on osteoblasts in culture. Zirconia improves cell proliferation significantly during the first days of culture, but it does not improve attachment and adhesion strength. Both materials do not differ with respect to protein synthesis or ultrastructural appearance of osteoblasts. Zirconium oxide may therefore be a suitable material for dental implants.

Osseointegration of zirconia implants: an SEM observation of the bone-implant interface.

BACKGROUND: The successful use of zirconia ceramics in orthopedic surgery led to a demand for dental zirconium-based implant systems. Because of its excellent biomechanical characteristics, biocompatibility, and bright tooth-like color, zirconia (zirconium dioxide, ZrO2) has the potential to become a substitute for titanium as dental implant material. The present study aimed at investigating the osseointegration of zirconia implants with modified ablative surface at an ultrastructural level. METHODS: A total of 24 zirconia implants with modified ablative surfaces and 24 titanium implants all of similar shape and surface structure were inserted into the tibia of 12 Göttinger minipigs. Block biopsies were harvested 1 week, 4 weeks or 12 weeks (four animals each) after surgery. Scanning electron microscopy (SEM) analysis was performed at the bone implant interface. RESULTS: Remarkable bone attachment was already seen after 1 week which increased further to intimate bone contact after 4 weeks, observed on both zirconia and titanium implant surfaces. After 12 weeks, osseointegration without interposition of an interfacial layer was detected. At the ultrastructural level, there was no obvious difference between the osseointegration of zirconia implants with modified ablative surfaces and titanium implants with a similar surface topography. CONCLUSION: The results of this study indicate similar osseointegration of zirconia and titanium implants at the ultrastructural level.