RESUMEN
Microplastics (MPs) in the aquatic environment pose a serious threat to biota, by being confounded with food. These effects occur in mussels which are filter-feeding organisms. Mussels from the genus Mytilus sp. were used to evaluate the ecotoxicological effects of two MPs, polypropylene (PP) and polyethylene terephthalate (PET), after 4 and 28-days. Measured individual endpoints were condition index and feeding rate; and sub-individual parameters, metabolism of phase I (CYP1A1, CYP1A2 and CYP3A4) and II (glutathione S-transferases - GSTs), and antioxidant defense (catalase - CAT). MPs decreased both condition index (CI) and feeding rate (FR). No alterations occurred in metabolic enzymes, suggesting that these MPs are not metabolized by these pathways. Furthermore, lack of alterations in GSTs and CAT activities suggests the absence of conjugation and oxidative stress. Overall, biochemical markers were not responsive, but non-enzymatic responses showed deleterious effects caused by these MPs, which may be of high ecological importance.
Asunto(s)
Ecotoxicología , Microplásticos , Mytilus , Contaminantes Químicos del Agua , Animales , Contaminantes Químicos del Agua/toxicidad , Microplásticos/toxicidad , Mytilus/efectos de los fármacos , Monitoreo del Ambiente , Glutatión Transferasa/metabolismo , Polipropilenos/toxicidad , Tereftalatos Polietilenos , Estrés Oxidativo , Catalasa/metabolismoRESUMEN
Human activities have directly impacted the environment, causing significant ecological imbalances. From the different contaminants resulting from human activities, plastics are of major environmental concern. Due to their high use and consequent discharge, plastics tend to accumulate in aquatic environments. There, plastics can form smaller particles (microplastics, MPs), due to fragmentation and weathering, which are more prone to interact with aquatic organisms and cause deleterious effects, including at the basis of different food webs. This study assessed the effects of two microplastics (polyethylene terephthalate, PET; and polypropylene, PP; both of common domestic use) in the freshwater cladoceran species Daphnia magna. Toxic effects were assessed by measuring reproductive traits (first brood and total number of offspring), and activities of biomarkers involved in xenobiotic metabolism (phase I: cytochrome P-450 isoenzymes CYP1A1, 1A2 and 3A4; phase II/conjugation: glutathione S-transferases; and antioxidant defense (catalase)). Both MPs showed a potential to significantly reduce reproductive parameters in D. magna. Furthermore, PET caused a significant increase in some isoenzymes of CYP450 in acutely exposed organisms, but this effect was not observed in chronically exposed animals. Similarly, the activity of the antioxidant defense (CAT) was significantly increased in acutely exposed animals, but not in chronically exposed organisms. This pattern of effects suggests a possible mechanism of long-term adaptation to the presence of the tested MPs. In conclusion, the herein tested MPs have shown the potential to induce deleterious effects on D. magna mainly observed in terms of the reproductive outcomes. Changes at the biochemical level seems transient and are not likely to occur in long term, environmentally exposed crustaceans.
Asunto(s)
Daphnia magna , Microplásticos , Reproducción , Contaminantes Químicos del Agua , Animales , Biomarcadores/metabolismo , Sistema Enzimático del Citocromo P-450/metabolismo , Daphnia magna/efectos de los fármacos , Glutatión Transferasa/metabolismo , Microplásticos/toxicidad , Polipropilenos/toxicidad , Reproducción/efectos de los fármacos , Contaminantes Químicos del Agua/toxicidadRESUMEN
Although the impacts of plastic pollution have long been recognized, the presence, pervasiveness, and ecotoxicological consequences of microplastic-i.e., plastic particles < 5 mm-contamination have only been explored over the last decade. Far less focus has been attributed to the role of these materials and, particularly, microplastics, as vectors for a multitude of chemicals, including those (un)intentionally added to plastic products, but also organic pollutants already present in the environment. Owing to the ubiquitous presence of microplastics in all environmental matrices and to the diverse nature of their chemical and physical characteristics, thoroughly understanding the mechanistic uptake/release of these compounds is inherently complex, but necessary in order to better assess the potential impacts of both microplastics and associated chemicals on the environment. Herein, we delve into the known processes and factors affecting these mechanisms. We center the discussion on microplastics and discuss some of the most prominent ecological implications of the sorption of this multitude of chemicals. Moreover, the key limitations of the currently available literature are described and a prospective outlook for the future research on the topic is presented.
RESUMEN
As the World slowly emerged from the then-ongoing pandemic, War broke out in Europe with the invasion of Ukraine by Russia. The enduring military conflict in Ukraine has had sweeping consequences at the human, social, economic, and environmental levels, not only for the nations involved but across Europe and globally. Damaged infrastructures, severe disruption of economic activity, and forced migration have led to negative impacts on sustainability. The COVID-19 pandemic has added another layer of complexity to this already challenging situation, as the virus has further disrupted economic activity and strained healthcare systems. Herein, we examine how the intersection of war and COVID-19 affect the United Nations' 2030 Agenda for Sustainable Development. How these intersecting challenges have impacted efforts to build a more sustainable future, and how these impacts have a global reach are also assessed. The broader implications of this case for understanding the linkages between conflict, pandemics, and sustainability more generally are also considered, relating these with the United Nations' Sustainable Development Goals (SDG) Agenda for 2030.
Asunto(s)
COVID-19 , Personal Militar , Humanos , Ucrania/epidemiología , Pandemias , COVID-19/epidemiología , Europa (Continente)RESUMEN
Cigarette butts (CBs) are non-biodegradable residues of synthetic origin, prevalent on beaches all over the world. The study evaluates discarded CBs on an intensely used urban beach, determining variations in physical and chemical characteristics. CBs collected were observed, classified, and visually separated according to a proposed scale of four levels of degradation to test the potential match between physical and chemical decay. CBs (un-smoked, smoked, and discarded) were used to determine the average length (cm) and mass (g) in order to observe changes in these parameters among the levels. Cigarette butts experience consecutive mass loss during environmental exposure. Scanning electron microscopy (SEM) images were obtained to assess physical changes in fibers due to smoking. FTIR-ATR was used to assess CBs new (un-smoked), smoked, and discarded samples in relation to cellulose acetate decay. The FTIR-ATR spectroscopy of the most visually degraded cigarette butts indicated modifications in the spectra when compared to un-smoked cigarettes.
Asunto(s)
Productos de Tabaco , Exposición a Riesgos Ambientales , Monitoreo del Ambiente , Humo/análisis , Fumar , NicotianaRESUMEN
The extraction of microplastics from organic-rich freshwater samples is challenging and limited information is available in the literature. This study aims at developing efficient methods for water volume reduction and organic matter removal in freshwater samples, while focusing on the reduction of the economic and environmental costs, maintaining microplastics integrity and avoiding contamination. For the water volume reduction approach, centrifuging freshwater samples (water, sediment, algae, leaves, driftwood, fish tissue) at different speeds (3500, 6000 rpm) and times (5, 10 min) showed that 3500 rpm for 5 min was efficient to settle the mineral and organic material, while preserving the polymers and showing high microplastic recovering rates (93 ± 6%). These recovery rates were significantly higher than the traditional sieving approach (77 ± 22%). The posterior minimal consumption of reagents resulting from the reduction of water volume helped to reduce the economic and environmental costs of the devised methodology, becoming more aligned with green chemistry principles. For biogenic organic matter removal, four digestion solutions were tested on freshwater samples, namely 10% potassium hydroxide, Fenton reagent (30% H2O2 + Fe(II)), 7% and 10% sodium hypochlorite (NaClO), under 3 periods of time (1, 6 and 15 h), at 50 °C. Both 7% and 10% NaClO showed the highest rates of organic matter removal (86 ± 1% and 90 ± 1%, respectively), after 6 h at 50 °C. Exposure of virgin and aged polymers (polyethylene, polypropylene, polystyrene, polyvinyl chloride, nylon, polyethylene terephthalate) to NaClO showed no weight, visual, surface structure, Fourier transform infrared spectra and carbonyl index changes, except for nylon, although not to an extent that affected its identification. This method resulted in high recovery rates of polymers (92 ± 6%). Thus, 7% NaClO at 50 °C for 6 h (or overnight) may be efficiently used for microplastic analysis in organic-rich freshwater samples.
Asunto(s)
Microplásticos , Contaminantes Químicos del Agua , Animales , Monitoreo del Ambiente , Agua Dulce , Peróxido de Hidrógeno , Plásticos , Contaminantes Químicos del Agua/análisisRESUMEN
Despite the increasing interest in microplastic (MP) research, the accurate prevalence, distribution and fate of these materials in the environment is yet poorly known and, consequently, a focus of debate. Hence, to better ascertain the presence of microplastics in specific environments, samples from 35 random sites distributed across a 4200-meter long section from the area of Sidi Mansour, Sfax-Tunisia, were collected and analyzed. MPs were extracted, digested with potassium hydroxide and dyed with Eosin B, for visual microscopy counting and sorting. Polymer composition and surface morphology were identified by FTIR-ATR spectroscopy and SEM microscopy. Total abundances ranged from 252 to-5332 particles per m2 where fragments and granules were the most frequent types of microplastics. These findings highlight the considerable presence of these materials in the studied harbor region and underscore the density dependence on the distribution and occurrence of MPs and how these tend to accumulate in the sandy sediments.
Asunto(s)
Sedimentos Geológicos/análisis , Plásticos/análisis , Contaminantes Químicos del Agua/análisis , Monitoreo del Ambiente/métodos , Microscopía Electrónica de Rastreo , Plásticos/química , Espectroscopía Infrarroja por Transformada de Fourier , Túnez , Contaminantes Químicos del Agua/químicaRESUMEN
Protein post-translational modifications (PTMs) are a key bacterial feature that holds the capability to modulate protein function and responses to environmental cues. Until recently, their role in the regulation of prokaryotic systems has been largely neglected. However, the latest developments in mass spectrometry-based proteomics have allowed an unparalleled identification and quantification of proteins and peptides that undergo PTMs in bacteria, including in species which directly or indirectly affect human health. Herein, we address this issue by carrying out the largest and most comprehensive global pooling and comparison of PTM peptides and proteins from bacterial species performed to date. Data was collected from 91 studies relating to PTM bacterial peptides or proteins identified by mass spectrometry-based methods. The present analysis revealed that there was a considerable overlap between PTMs across species, especially between acetylation and other PTMs, particularly succinylation. Phylogenetically closer species may present more overlapping phosphoproteomes, but environmental triggers also contribute to this proximity. PTMs among bacteria were found to be extremely versatile and diverse, meaning that the same protein may undergo a wide variety of different modifications across several species, but it could also suffer different modifications within the same species.
Asunto(s)
Bacterias/química , Proteínas Bacterianas/metabolismo , Procesamiento Proteico-Postraduccional , Acetilación , Recolección de Datos/métodos , Humanos , Espectrometría de Masas , Péptidos/metabolismo , Fosfoproteínas , Fosforilación , Filogenia , Proteómica/métodos , Ácido Succínico/metabolismoRESUMEN
There have been extensive and comprehensive reviews in the field of metal sulfide precipitation in the context of environmental remediation. However, these works have focused mainly on the removal of metals from aqueous solutions-usually, metal-contaminated effluents-with less emphasis on the precipitation process and on the end-products, frequently centering on metal removal efficiencies. Recently, there has been an increasing interest not only in the possible beneficial effects of these bioremediation strategies for metal-rich effluents but also on the formed precipitates. These metal sulfide materials are of special relevance in industry, due to their optical, electronic, and mechanical properties. Hence, identifying new routes for synthesizing these materials, as well as developing methodologies allowing for the control of the shape and size of particulates, is of environmental, economic, and practical importance. Multiple studies have shown proof-of-concept for the biological synthesis of inorganic metallic sulfide nanoparticles (NPs), resorting to varied organisms or cell components, though this information has scarcely been structured and compiled in a systematic manner. In this review, we overview the biological synthesis methodologies of nanosized metal sulfides and the advantages of these strategies when compared to more conventional chemical routes. Furthermore, we highlight the possibility of the use of numerous organisms for the synthesis of different metal sulfide NPs, with emphasis on sulfate-reducing bacteria (SRB). Finally, we put in perspective the potential of these methodologies in the emerging research areas of biohydrometallurgy and nanobiotechnology for the uptake of metals in the form of metal sulfide nanoparticles. A more complete understanding of the principles underlying the (bio)chemistry of formation of solids in these conditions may lead to the large-scale production of such metal sulfides, while simultaneously allowing an enhanced control over the size and shape of these biogenic nanomaterials.
Asunto(s)
Biotecnología/métodos , Nanopartículas del Metal , Semiconductores , Sulfuros/metabolismo , Bacterias/metabolismo , Biotecnología/tendencias , Precipitación QuímicaRESUMEN
Answering the question as to why we age is tantamount to answering the question of what is life itself. There are countless theories as to why and how we age, but, until recently, the very definition of aging - senescence - was still uncertain. Here, we summarize the main views of the different models of senescence, with a special emphasis on the biochemical processes that accompany aging. Though inherently complex, aging is characterized by numerous changes that take place at different levels of the biological hierarchy. We therefore explore some of the most relevant changes that take place during aging and, finally, we overview the current status of emergent aging therapies and what the future holds for this field of research. From this multi-dimensional approach, it becomes clear that an integrative approach that couples aging research with systems biology, capable of providing novel insights into how and why we age, is necessary.
Asunto(s)
Envejecimiento/genética , Envejecimiento/metabolismo , Envejecimiento/patología , Animales , Predicción , Depuradores de Radicales Libres/farmacología , Humanos , Especies Reactivas de Oxígeno/antagonistas & inhibidores , Especies Reactivas de Oxígeno/metabolismo , Trasplante de Células Madre/tendenciasRESUMEN
The dawn of a new Proteomics era, just over a decade ago, allowed for large-scale protein profiling studies that have been applied in the identification of distinctive molecular cell signatures. Proteomics provides a powerful approach for identifying and studying these multiple molecular markers in a vast array of biological systems, whether focusing on basic biological research, diagnosis, therapeutics, or systems biology. This is a continuously expanding field that relies on the combination of different methodologies and current advances, both technological and analytical, which have led to an explosion of protein signatures and biomarker candidates. But how are these biological markers obtained? And, most importantly, what can we learn from them? Herein, we briefly overview the currently available approaches for obtaining relevant information at the proteome level, while noting the current and future roles of both traditional and modern proteomics. Moreover, we provide some considerations on how the development of powerful and robust bioinformatics tools will greatly benefit high-throughput proteomics. Such strategies are of the utmost importance in the rapidly emerging field of immunoproteomics, which may play a key role in the identification of antigens with diagnostic and/or therapeutic potential and in the development of new vaccines. Finally, we consider the present limitations in the discovery of new signatures and biomarkers and speculate on how such hurdles may be overcome, while also offering a prospect for the next few years in what could be one of the most significant strategies in translational medicine research.
Asunto(s)
Proteoma/análisis , Proteómica/métodos , Ensayos Analíticos de Alto Rendimiento/métodosRESUMEN
Chemotherapy continues to be the standard treatment for advanced or metastasized cancer. However, commonly used chemotherapeutic agents may induce damage in healthy cells and tissues. Thus, in recent years, there has been an increased focus on the development of new, efficient anticancer drugs exhibiting low toxicity and that are not affected by mechanisms of chemoresistance. In the present work, we tested synthetic and naturally obtained human salivary peptides against breast, prostate, colon, osteosarcoma and bladder cancer cell lines (T47-D, PC-3, HT-29, MG63, T-24, respectively). Results have showed that there is a reduced cell population increase that is peptide-, cell- and possibly pathway-specific, with the most potent effect observed in observed in T-47D breast cancer cells. Protein expression and microscopy results further indicate that, in this cell line, the peptide with the sequence GPPPQGGRPQG (GG peptide) interferes with the ability of cell adhesion proteins to stabilize adherens junctions, such as E-cadherin, leading to apoptosis. These promising results encourage future works aimed at disclosing the vast potential of salivary peptides as new therapeutic agents.
Asunto(s)
Antineoplásicos/química , Antineoplásicos/farmacología , Apoptosis/efectos de los fármacos , Neoplasias/tratamiento farmacológico , Proteínas y Péptidos Salivales/química , Proteínas y Péptidos Salivales/farmacología , Uniones Adherentes/metabolismo , Cadherinas/metabolismo , Adhesión Celular/efectos de los fármacos , Línea Celular Tumoral , Humanos , Proteínas de Neoplasias/metabolismo , Neoplasias/metabolismo , Neoplasias/patologíaRESUMEN
Antimicrobial peptides are small molecules with activity against bacteria, yeasts, fungi, viruses, bacteria, and even tumor cells that make these molecules attractive as therapeutic agents. Due to the alarming increase of antimicrobial resistance, interest in alternative antimicrobial agents has led to the exploitation of antimicrobial peptides, both synthetic and from natural sources. Thus, many peptide-based drugs are currently commercially available for the treatment of numerous ailments, such as hepatitis C, myeloma, skin infections, and diabetes. Initial barriers are being increasingly overcome with the development of cost-effective, more stable peptides. Herein, we review the available strategies for their synthesis, bioinformatics tools for the rational design of antimicrobial peptides with enhanced therapeutic indices, hurdles and shortcomings limiting the large-scale production of AMPs, as well as the challenges that the pharmaceutical industry faces on their use as therapeutic agents.
Asunto(s)
Péptidos Catiónicos Antimicrobianos/uso terapéutico , Productos Biológicos/uso terapéutico , Enfermedades Transmisibles/tratamiento farmacológico , Péptidos Catiónicos Antimicrobianos/aislamiento & purificación , Péptidos Catiónicos Antimicrobianos/farmacología , Productos Biológicos/aislamiento & purificación , Productos Biológicos/farmacología , Biotecnología/métodos , Biotecnología/tendencias , Descubrimiento de Drogas/métodos , Descubrimiento de Drogas/tendencias , Humanos , Tecnología Farmacéutica/métodos , Tecnología Farmacéutica/tendenciasRESUMEN
Formed as an interdisciplinary domain on the basis of Human Genome Project, Proteomics aims at the large-scale study of proteins. The enthusiasm that resulted from obtaining the complete human genetic information has, however, been chastened by the realization that this information contributes little to the comprehension and knowledge of the expressed proteins. In the wake of this realization, the Human Proteome Project (HUPO) was founded, which is a global, collaborative initiative, aiming at the complete characterization of the proteins of all protein-coding genes. Nonetheless, the rapid detection of these molecules in complex biological samples under conditions considered to be of clinical relevance is extremely difficult, requiring the development of very sensitive, robust, reproducible and high throughput platforms. Nanoproteomics has emerged as a feasible, promising option, offering short assay times, low sample consumption, ultralow detection and high throughput capacity. Additionally, the successful synthesis of biomolecules and nanoparticle hybrids yields systems which often exhibit new or improved features. Herein, we overview the recent advances in bioconjugation at the nanolevel and, specifically, their application in Proteomics, discussing not only the merits and prospects of Proteomics, but also present day limitations.
Asunto(s)
Materiales Biocompatibles/química , Nanoestructuras/química , Nanotecnología/métodos , Proteómica/métodos , Animales , HumanosRESUMEN
Phosphatidylethanolamines are a major class of phospholipids found in cellular membranes. Identification of the alterations in these phospholipids, induced by free radicals, could provide new tools for in vivo diagnosis of oxidative stress. In this study, 1-palmitoyl-2-linoleoyl-phosphatidylethanolamine oxidation products, induced by the hydroxyl radical, were studied using LC-MS and LC-MS/MS. Data obtained allowed the identification and separation of isomeric oxidative products with modifications in the sn-2 acyl chain, attributed to long- and short-chain products. Among long-chain products keto, keto-hydroxy, hydroxy, poly-hydroxy, peroxy and hydroxy-peroxy derivatives were identified. Product ions formed by loss of two H2O molecules vs loss of HOOH, allowed the identification of, respectively, di- (or poli-) hydroxy vs peroxy derivatives. Location of functional groups was determined by the product ions formed by cleavage of C-C bonds, in the vicinity of the oxidation positions, allowing the identification of C9, C12 and C13 as the predominant substituted positions. Short-chain products identified comprised aldehydes, hydroxy-aldehydes and carboxylic derivatives, with modified sn-2 acyl lengths of C7-C9 and C11, C12. Among the short-chain products identified, C9 products showed higher relative abundance.