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1.
Animals (Basel) ; 13(21)2023 Oct 26.
Artículo en Inglés | MEDLINE | ID: mdl-37958093

RESUMEN

One of the main factors limiting tilapia's production is the occurrence of infections caused by Aeromonas and Streptococcus species. This work intended to evaluate a bivalent vaccine against A. hydrophila and S. agalactiae by intraperitoneal (i.p) administration in Nile tilapia (Oreochromis niloticus) in Brazil. The study was carried out in two phases: one in the laboratory, on a small scale, and from the results obtained, the study was expanded to a large scale in a production system in cages. The vaccine proved to be safe and effective in laboratory tests, with a vaccine efficacy (VE) of 93.66%. However, in large-scale tests with 12,000 tilapias, the VE was 59.14%, with a better food conversion ratio (1.54 kg) in the vaccinated group compared to the control group (1.27 kg). These results corroborate the efficiency of this tested vaccine; however, they indicate the need for field tests to attest to real protection.

2.
Genome Biol ; 24(1): 191, 2023 08 28.
Artículo en Inglés | MEDLINE | ID: mdl-37635261

RESUMEN

BACKGROUND: In humans, muscle-invasive bladder cancer (MIBC) is highly aggressive and associated with a poor prognosis. With a high mutation load and large number of altered genes, strategies to delineate key driver events are necessary. Dogs and cats develop urothelial carcinoma (UC) with histological and clinical similarities to human MIBC. Cattle that graze on bracken fern also develop UC, associated with exposure to the carcinogen ptaquiloside. These species may represent relevant animal models of spontaneous and carcinogen-induced UC that can provide insight into human MIBC. RESULTS: Whole-exome sequencing of domestic canine (n = 87) and feline (n = 23) UC, and comparative analysis with human MIBC reveals a lower mutation rate in animal cases and the absence of APOBEC mutational signatures. A convergence of driver genes (ARID1A, KDM6A, TP53, FAT1, and NRAS) is discovered, along with common focally amplified and deleted genes involved in regulation of the cell cycle and chromatin remodelling. We identify mismatch repair deficiency in a subset of canine and feline UCs with biallelic inactivation of MSH2. Bovine UC (n = 8) is distinctly different; we identify novel mutational signatures which are recapitulated in vitro in human urinary bladder UC cells treated with bracken fern extracts or purified ptaquiloside. CONCLUSION: Canine and feline urinary bladder UC represent relevant models of MIBC in humans, and cross-species analysis can identify evolutionarily conserved driver genes. We characterize mutational signatures in bovine UC associated with bracken fern and ptaquiloside exposure, a human-linked cancer exposure. Our work demonstrates the relevance of cross-species comparative analysis in understanding both human and animal UC.


Asunto(s)
Carcinoma de Células Transicionales , Enfermedades de los Gatos , Enfermedades de los Perros , Neoplasias de la Vejiga Urinaria , Humanos , Animales , Gatos , Bovinos , Perros , Neoplasias de la Vejiga Urinaria/genética , Carcinógenos , Músculos
3.
Acta Parasitol ; 67(2): 648-657, 2022 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-34988857

RESUMEN

PURPOSE: Leishmaniasis are infectious and zoonotic diseases and present in cutaneous and visceral forms. Cutaneous leishmaniasis is endemic and widely distributed throughout the state of Espírito Santo, Brazil. Several cases of cutaneous leishmaniasis in humans and dogs associated with Leishmania (Viannia) braziliensis have been reported in the state. This study aimed to investigate the occurrence of natural infection by Leishmania(Viannia) braziliensis in wild mammals found dead (by trampling or natural death) in the Sooretama Biological Reserve, Espírito Santo State. METHODS: From January 2018 to December 2019, 60 animals were collected. Of these, 47 animals from 12 different species were analyzed. The results were demonstrated using descriptive analysis of the observations to calculate the absolute and relative frequencies of the data. RESULTS: In the PCR, using specific primers for the genus Leishmania (D1, D2, and D3) and the species Leishmania (Viannia) braziliensis (ISVB/ISVC), 4 positive animals (8.5%) were detected: 1 Cuniculus paca (paca) (25%) and 3 Callithrix geoffroyi (white-faced marmoset) (25%). In the histopathological analysis, the parasitic amastigote form was not observed. CONCLUSIONS: The natural infection, detected by PCR, by Leishmania (Viannia) braziliensis in Cuniculus paca (paca) and Callithrix geoffroyi (white-faced marmoset) constitutes the first report of infection of this rodent and primate species in the literature. Despite the confirmation of Leishmania (Viannia) braziliensis infection in rodents and primates, the role of these species in the transmission of this zoonosis still needs further observational studies to identify their seasonal variation, transmissibility, infection stability, and the effects of a given parasite on the population and/or individual.


Asunto(s)
Leishmania braziliensis , Leishmania , Leishmaniasis Cutánea , Animales , Brasil/epidemiología , Leishmaniasis Cutánea/epidemiología , Leishmaniasis Cutánea/parasitología , Leishmaniasis Cutánea/veterinaria , Mamíferos/parasitología , Roedores , Zoonosis/epidemiología
4.
Heliyon ; 7(2): e06092, 2021 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-33659725

RESUMEN

Dioctophyme renale is the largest parasitic nematode of animals. It is most often found in the right kidney, but can also occur in the urinary system, ears, free abdominal cavity, mammary gland, thoracic cavity, and more rarely in subcutaneous tissue. The genus Dirofilaria has specific parasitic characteristics according to its location, and may parasitize the respiratory tract or even the skin, varying according to species. This report describes a case of Dioctophyme renale and Dirofilaria sp. in the subcutaneous tissue of a cat in the state of Espírito Santo, Brazil. An adult male mongrel cat showed physical enlargement in the left inguinal region, diagnosed as a subcutaneous nodule. Surgical excision and histopathological evaluation of the nodule were performed, and Dioctophyme renale and Dirofilaria sp. were found inside. Dioctophymosis and heartworm disease are present in Brazil and most other countries, but this is the unprecedented case of the association of Dioctophyme renale and Dirofilaria sp. in the subcutaneous tissue of a cat.

5.
Anat Histol Embryol ; 50(2): 350-359, 2021 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-33249637

RESUMEN

The broad-snouted caiman, Caiman latirostris (Daudin, 1802), is one of the six crocodilian species from Brazil. The topography, morphology and morphometry of the broad-snouted caiman heart were studied. Data were obtained from the necropsy of four adult animals, three females and one male. The hearts were removed from the coelomic cavity and fixed in 10% formalin for 48 hr for morphological and morphometric description. The heart is in the cranial mediastinum. It is caudally involved by the liver cranial margins, and ventrally by the ribs, intercostal muscles, and sternum and dorsally by the lungs. The four-chambered morphology is typical with two (right and left) atria and ventricles. Right and left aortic, pulmonary and subclavian arteries branch from the truncus arteriosus. Gubernaculum cordis is present as ligamentous folds uniting the heart apex to the pericardium. Main morphometric means are the apex-to-base length (49.86 mm), circumference (105.25 mm) and heart weight (45.03 g). The right atrium is craniocaudally longer with thicker walls, whereas the left ventricle is narrower. The topography, morphology and morphometry of the heart of C. latirostris are consistent with the anatomy of other crocodilian species.


Asunto(s)
Caimanes y Cocodrilos , Animales , Brasil , Femenino , Masculino
6.
J Sci Food Agric ; 100(15): 5442-5449, 2020 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-32567144

RESUMEN

BACKGROUND: Yacón (Smallanthus sonchifolius) roots store carbohydrate in the form of prebiotic fructooligosaccharides (FOS), which improve intestinal health. Yacon has the potential to prevent the intestinal barrier alterations associated with colorectal cancer (CRC). This study aimed to investigate the preventive effects of yacón flour (YF) on alterations promoted by CRC induced by 1,2-dimethylhydrazine in rats. RESULTS: CRC increased tumor necrosis factor alpha levels (group CY = 10.2 ± 0.72; group C = 9.6 ± 1.0; group Y = 5.8 ± 0.54; group S = 5.95 ± 0.6 pg mL-1 ) and short-chain fatty acid production, and decreased total antioxidant capacity (group CY = 4.7 ± 0.72; group C = 3.3 ± 0.3; group Y = 4.1 ± 0.47; group S = 6.7 ± 0.78 U mL-1 ). Furthermore, YF treatment reduced intraluminal pH (group CY = 6.45 ± 0.47; group C = 7.65 ± 0.44; group Y = 6.75 ± 0.46; group S = 8.13 ± 0.2), lactulose/mannitol ratio, tumor necrosis factor-alpha (TNF-α)/interleukin (IL)-10 ratio, and increased secretory immunoglobulin A (group CY = 9.48 ± 1.46; group C = 10.95 ± 3.87; group Y = 15.95 ± 7.36; group S = 9.19 ± 1.52), but did not affect IL-10, IL-12, and TNF-α levels nor the IL-12/IL-10 ratio. CONCLUSION: YF as a source of fructooligosaccharides may help to maintain the integrity of intestinal health, which is altered in induced CRC in rats. © 2020 Society of Chemical Industry.


Asunto(s)
Asteraceae/química , Neoplasias Colorrectales/metabolismo , Neoplasias Colorrectales/prevención & control , Oligosacáridos/metabolismo , Estrés Oxidativo/efectos de los fármacos , Extractos Vegetales/metabolismo , Animales , Carcinogénesis , Neoplasias Colorrectales/genética , Neoplasias Colorrectales/inmunología , Modelos Animales de Enfermedad , Ácidos Grasos Volátiles/metabolismo , Humanos , Interleucina-10/genética , Interleucina-10/inmunología , Intestinos/efectos de los fármacos , Intestinos/inmunología , Masculino , Raíces de Plantas/química , Prebióticos/análisis , Ratas , Ratas Wistar , Factor de Necrosis Tumoral alfa/genética , Factor de Necrosis Tumoral alfa/inmunología
7.
Toxicon ; 171: 20-28, 2019 Dec 05.
Artículo en Inglés | MEDLINE | ID: mdl-31542469

RESUMEN

The indolizidine alkaloid swainsonine, found in some Astragalus and Oxytropis (i.e., locoweed) species, is a potent cellular glycosidase inhibitor that often poisons livestock. Other toxic genera such as some Ipomoea species also contain swainsonine as well as calystegines which are similar polyhydroxy alkaloids. The toxicity of calystegines is poorly characterized; however, they are also potent glycoside inhibitors capable of intestinal and cellular glycoside dysfunction. The objective of this study was to directly compare A. lentiginosus and I. carnea poisoning in goats to better characterize the role of the calystegines. Three groups of four goats each were treated with ground alfalfa (control), I. carnea or A. lentiginosus to obtain daily doses of 0.0, 1.5, and 1.5 mg swainsonine/kg bw per day, respectively, for 45 days. Animals were observed daily and weekly body weights, serum enzyme activities, and serum swainsonine concentrations were determined. At day 45 all animals were euthanized and necropsied. Goats treated with A. lentiginosus and I. carnea developed clinical disease characterized by mild intention tremors and proprioceptive deficits. Goats treated with A. lentiginosus developed clinical disease sooner and with greater consistency. No differences in body weight, serum swainsonine concentrations and serum enzyme activity were observed between goats treated with A. lentiginosus and I. carnea. Additionally, there were no differences in the microscopic and histochemical studies of the visceral and neurologic lesions observed between goats treated with A. lentiginosus and I. carnea. These findings suggest that I. carnea-induced clinical signs and lesions are due to swainsonine and that calystegines contribute little or nothing to toxicity in goats in the presence of swainsonine.


Asunto(s)
Planta del Astrágalo/envenenamiento , Enfermedades de las Cabras/etiología , Ipomoea/envenenamiento , Intoxicación por Plantas/veterinaria , Swainsonina/envenenamiento , Animales , Enfermedades de las Cabras/enzimología , Enfermedades de las Cabras/patología , Cabras , Masculino , Propiocepción/efectos de los fármacos , Swainsonina/sangre , Temblor/veterinaria , Tropanos/envenenamiento
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