Social Inclusion and Pest Management: a Route for Improved Food Production and well-being in the Global South.

World trade has facilitated the spread of non-native pest species, presenting new challenges for food production. In the Global South, linking worker social protection programs with invasive pest management can, at the same time, contribute to food security and empower workers. The spotted wing Drosophila (Drosophila suzukii) (SWD) recent invasion in the Global South case study illustrates how science-based policies integrated with agricultural worker-based social organizations can contribute toward economic and environmental sustainability.

Oviposition substrate location by the invasive woodwasp Sirex noctilio: the combined effect of chemical cues emitted by its obligate symbiont Amylostereum areolatum and different host-tree species.

BACKGROUND: Sirex noctilio is an invasive forest wasp that affects pines and can result in severe economic losses. The use of semiochemicals offers an opportunity to develop sensitive and specific capturing systems to mitigatenegative impacts. Previous research showed that female S. noctilio would use volatiles emitted by its fungal symbiont, Amylostereum areolatum, but little is known about how these modulate behaviour when combined with pine-wood emissions. Our aim was to understand the relevance of fungal volatiles grown on artificial media and wood from two hosts trees, Pinus contorta and Pinus ponderosa, on behavioural and electroantennographic responses of wasp females. Because background odours can modify an insect's response towards resource-indicating semiochemicals, we propose that the behaviour towards the symbiont (resource) will be modulated by host pine emissions (background odours). RESULTS: Olfactometric assays showed that both host species with fungus were attractive when contrasted against air (P. contorta versus Air, χ2 = 12.19, P < 0.001; P. ponderosa versus Air, χ2 = 20.60, P < 0.001) and suggest a clear hierarchy in terms of female preferences towards the tested stimuli, with response highest towards the fungus grown on P. contorta (olfactory preference index: 5.5). Electrophysiological analyses indicate that females detect 62 volatile compounds from the tested sources. CONCLUSION: Results indicate a strong synergy between symbiont and host semiochemicals, suggesting that the pine species could play a fundamental role in the interaction. Further understanding of the chemical basis of this, could guide the development of specific and attractive lures, in order to maximize attraction of wasps in surveillance programmes. © 2023 Society of Chemical Industry.

Integrated Pest Management Education: a Video-Game to Improve Management of Drosophila suzukii, Soft-Skin Fruit Pest.

The transition from the conventional global agriculture to an agroecological model poses a teaching and learning challenge to facilitate the wide variety of practices and the many biological processes involved. Games, such as board games, video-games, or mobile apps, are elements that may be used for teaching agroecology, environmental education, or animal conservation. Here, we present a video-game designed to contribute to integrated pest management education. The Spotted-Stop-It video-game is a single-player game which encourages participation, disseminates knowledge on the pest problem and its potential solutions (i.e., harvest frequency, sanitation, and management of alternative non-crop plants), and highlights the importance of good practices from an agroecological perspective at the farmer scale. In a farm-tech regional fair, we presented the game to its users and performed a simple retrospective survey. The survey results showed that most participants did not know about the fly prior to playing the game (34 ind., 68%), but were able to recognize the species among other flies after playing (23 ind., ~ 65%). Also, 21 individuals correctly responded about the effects of this pest on soft-skin fruits ("the fly lays eggs inside the fruit"). The training of future generations on new insect invasions and IPM practices with elements of their own environment may prove to be important to transmit concepts and practices at the service of sustainable crop protection.

Tipo de edentulismo parcial bimaxilar y su asociación con el nivel socioeconómico-cultural / Type of bimaxillary partial edentulism and its association with socioeconomic cultural level

Introducción: El edentulismo parcial es una condición final a la presencia de caries dental relacionado con diversos factores sociales, demográficos y culturales. Actualmente, existen escasos estudios que valoren el estado de edentulismo parcial y su asociación con el nivel socioeconómico-cultural. Objetivo: Determinar la frecuencia del tipo de edentulismo parcial bimaxilar según la Clase de Kennedy y su posible asociación con el nivel socioeconómico-cultural en los pacientes de la clínica de la Facultad de Odontología de la Universidad Nacional Mayor de San Marcos del año 2016. Material y Métodos: Estudio descriptivo, observacional y transversal. La muestra estuvo conformada por 100 pacientes adultos edéntulos parciales seleccionados aleatoriamente que fueron evaluados mediante una encuesta y un examen clínico oral. La encuesta estuvo conformada por los datos sobre el nivel socioeconómico-cultural. El examen clínico se evaluó con un odontograma para determinar el tipo de edentulismo según la clasificación de Kennedy. Resultados: El tipo de edentulismo parcial más frecuente fue la Clase III de Kennedy en la arcada maxilar (50 por ciento) y en la mandibular (49 por ciento), los portadores pertenecían en gran medida al nivel socioeconómico-cultural medio (83 por ciento). No se evidenció una asociación estadísticamente significativa entre el nivel socioeconómico-cultural y el tipo de edentulismo parcial según la Clase de Kennedy en ambas arcadas dentarias. Conclusiones: En la población estudiada existe una mayor frecuencia de edentulismo parcial bimaxilar Clase III de Kennedy y una preponderancia del nivel socioeconómico-cultural medio. Sin embargo, no existe asociación entre el tipo de edentulismo parcial según la Clase de Kennedy y el nivel socioeconómico-cultural(AU)

Introduction: Partial edentulism is a final condition to the presence of dental caries related to various social, demographic and cultural factors. Currently, there are few studies that assess the state of partial edentulism and its association with the socioeconomic cultural level. Objective: To determine the frequency of the type of bimaxillary partial edentulism according to Kennedy´s Class and its possible association with the socioeconomic cultural level in patients treated in the clinic of the Faculty of Dentistry of the National University of San Marcos in 2016. Material and methods: A descriptive, observational and cross-sectional study was conducted. The sample consisted of 100 randomly selected partial edentulous adult patients who were evaluated by means of a survey and an oral clinical examination. The survey was made up of the data that relate socioeconomic cultural level. The clinical examination was evaluated with an odontogram to determine the type of edentulism according to Kennedy´s classification. Results: The most frequent type of partial edentulism was Kennedy's Class III in the maxillary arch (50 percent) and in the mandible (49 percent), which was mainly present in patients that belonged to the average socioeconomic cultural level (83 percent). There was no statistically significant association between the socioeconomic cultural level and the type of partial edentulism according to Kennedy´s Class in both dental arches. Conclusions: In the studied population, there is a greater frequency of partial bimaxilar edentulism according to Kennedy's Class III and a preponderance of the socioeconomic cultural level. However, there is no association between the type of partial edentulism according to Kennedy´s Class and the socioeconomic cultural level(AU)

Thermal Tolerance Plasticity in Chagas Disease Vectors Rhodnius prolixus (Hemiptera: Reduviidae) and Triatoma infestans.

Temperature is recognized as the most influential abiotic factor on the distribution and dispersion of most insect species including Rhodnius prolixus (Stål, 1859) and Triatoma infestans (Klug, 1834), the two most important Chagas disease vectors. Although, these species thermotolerance range is well known their plasticity has never been addressed in these or any other triatomines. Herein, we investigate the effects of acclimation on thermotolerance range and resistance to stressful low temperatures by assessing thermal critical limits and 'chill-coma recovery time' (CCRT), respectively. We found positive effects of acclimation on thermotolerance range, especially on the thermal critical minimum of both species. In contrast, CCRT did not respond to acclimation in either. Our results reveal the plasticity of these Triatomines thermal tolerance in response to a wide range of acclimation temperatures. This presumably represents a physiological adaptation to daily or seasonal temperature variation with concomitant improvement in dispersion potential.

Genetic variation for tolerance to high temperatures in a population of Drosophila melanogaster.

The range of thermal tolerance is one of the main factors influencing the geographic distribution of species. Climate change projections predict increases in average and extreme temperatures over the coming decades; hence, the ability of living beings to resist these changes will depend on physiological and adaptive responses. On an evolutionary scale, changes will occur as the result of selective pressures on individual heritable differences. In this work, we studied the genetic basis of tolerance to high temperatures in the fly Drosophila melanogaster and whether this species presents sufficient genetic variability to allow expansion of its upper thermo-tolerance limit. To do so, we used adult flies derived from a natural population belonging to the Drosophila Genetic Reference Panel, for which genomic sequencing data are available. We characterized the phenotypic variation of the upper thermal limit in 34 lines by measuring knockdown temperature (i.e., critical thermal maximum [CTmax]) by exposing flies to a ramp of increasing temperature (0.25°C/min). Fourteen percent of the variation in CTmax is explained by the genetic variation across lines, without a significant sexual dimorphism. Through a genomewide association study, 12 single nucleotide polymorphisms associated with the CTmax were identified. In most of these SNPs, the less frequent allele increased the upper thermal limit suggesting that this population harbors raw genetic variation capable of expanding its heat tolerance. This potential upper thermal tolerance increase has implications under the global warming scenario. Past climatic records show a very low incidence of days above CTmax (10 days over 25 years); however, future climate scenarios predict 243 days with extreme high temperature above CTmax from 2045 to 2070. Thus, in the context of the future climate warming, rising temperatures might drive the evolution of heat tolerance in this population by increasing the frequency of the alleles associated with higher CTmax.

Using eco-physiological traits to understand the realized niche: the role of desiccation tolerance in Chagas disease vectors.

Small ectotherms, such as insects, with high surface area-to-volume ratios are usually at risk of dehydration in arid environments. We hypothesize that desiccation tolerance in insects could be reflected in their distribution, which is limited by areas with high relative values of water vapor pressure deficit (VPD) (e.g., hot and dry). The main goal of this study was to explore whether incorporation of eco-physiological traits such as desiccation tolerance in arid environments can improve our understanding of species distribution models (SDM). We use a novel eco-physiological approach to understand the distribution and the potential overlap with their fundamental niche in triatomine bugs, Chagas disease vectors. The desiccation dimension for T. infestans, T. delpontei, T. dimidiata, and T. sordida niches seems to extend to very dry areas. For T. vitticeps, xeric areas seem to limit the geographical range of their realized niche. The maximum VPD limits the western and southern distributions of T. vitticeps, T. delpontei, and T. patagonica. All species showed high tolerance to desiccation with survival times (35 °C-RH ~ 15%) ranging from 24 to 38 days, except for T. dimidiata (9 days), which can be explained by a higher water-loss rate, due to a higher cuticular permeability along with a higher critical water content. This approach indicates that most of these triatomine bugs could be exploiting the dryness dimension of their fundamental niche. Incorporating such species-specific traits in studies of distribution, range, and limits under scenarios of changing climate could enhance predictions of movement of disease-causing vectors into novel regions.

Enhanced fertility and chill tolerance after cold-induced reproductive arrest in females of temperate species of the Drosophila buzzatii complex.

Long-term exposure to low temperatures during adult maturation might decrease fertility after cold recovery as a consequence of carry-over effects on reproductive tissues. This pattern should be more pronounced in tropical than in temperate species as protective mechanisms against chilling injuries are expected to be more effective in the latter. We initially determined the lower thermal thresholds to induce ovarian maturation in four closely related Drosophila species, two inhabiting temperate regions and the other two tropical areas of South America. As expected, only temperate species regularly experience cold-inducing conditions for reproductive arrest during winter in their natural environment. Subsequently, we exposed reproductively arrested and mature females to cold-inducing conditions for reproductive arrest over a long period. Following cold exposure, tropical species exhibited a dramatic fertility decline, irrespective of reproductive status. In contrast, not only were temperate females fecund and fertile but also fertility was superior in females that underwent cold-induced reproductive arrest, suggesting that it might act as a protecting mechanism ensuring fertility after cold recovery. Based on these findings, we decided to evaluate the extent to which reproductive status affects cold tolerance and energy metabolism at low temperature. We found a lower metabolic rate and a higher cold tolerance in reproductively arrested females, although only temperate species attained high levels of chill tolerance. These findings highlight the role of cold-induced reproductive arrest as part of an integrated mechanism of cold adaptation that could potentially contribute to the spread of temperate species into higher latitudes or altitudes.