RESUMEN
This article deals with the synthesis of Schiff-based bis-azomethine-based ligands derived from pyridoxal and aliphatic dihydrazides and the synthesis of nickel(II) complexes C1-C4. The synthesized complexes had their structures elucidated by monocrystal X-ray diffraction and were characterized by vibrational and absorption spectroscopy. The synthesized ligands have characteristics that allow the formation of self-assembly processes, thus, the flexibility or rigidity of the coordination of organic molecules added to the orbitals of the NiII cation leads to the formation of helical complexes with double helix and a dinucler nickel(II) complex. Moreover, compounds was their interactions with CT-DNA and HSA absorption and emission analysis and molecular docking calculations.
Asunto(s)
Complejos de Coordinación/química , Níquel/química , Piridoxal/química , Adipatos/química , Compuestos Azo/química , Cristalografía por Rayos X/métodos , ADN/química , Humanos , Hidrazinas/química , Ligandos , Simulación del Acoplamiento Molecular/métodos , Estructura Molecular , Bases de Schiff/química , Albúmina Sérica Humana/química , Solubilidad , Succinatos/química , Tiosemicarbazonas/química , Agua/química , Difracción de Rayos X/métodosRESUMEN
Biodegradable films were produced from blends contained a high amount of thermoplastic starch (TPS) and poly(lactic acid) (PLA) plasticized with different adipate or citrate esters. It was not possible to obtain pellets for the production of films using only glycerol as a plasticizer. The plasticization of the PLA with the esters and mixture stages added through extrusion was critical to achieve a blend capable of producing films by blow extrusion. Adipate esters were the most effective plasticizers because they interacted best with the PLA and yielded films with appropriate mechanical properties.
Asunto(s)
Ácido Cítrico , Ésteres , Ácido Láctico , Polímeros , Almidón , Adipatos/química , Biodegradación Ambiental , Ácido Cítrico/síntesis química , Ácido Cítrico/química , Ésteres/síntesis química , Ésteres/química , Ácido Láctico/síntesis química , Ácido Láctico/química , Plásticos , Poliésteres , Polímeros/síntesis química , Polímeros/química , Almidón/síntesis química , Almidón/químicaRESUMEN
A conjugate meningococcal vaccine against serogroup B/C consisting of capsular PS (polysaccharide) from serogroup C conjugated to OMV (outer membrane vesicle) from serogroup B would be a very useful vaccine in regions where there is a prevalence of both serogroups, for example in Brazil. For this purpose, the conjugation method that uses ADHy (adipic acid dihydrazide) as spacer and a carbodi-imide derivative, EDAC [1-ethyl-3-(3-dimethylaminopropyl)carbodi-imide], as catalyser was optimized looking for synthesis yield and maintenance of the antigenicity of both components. The best synthesis conditions preserving the vaccine immunogenicity resulted in a final yield of approx. 17%. Immunogenicity of the vaccine was highest when 10% of the sialic acid residues of the PS were occupied by the ADHy spacer. Sterilization of the conjugate by filtration through a 0.22-microm-pore-size membrane resulted in a low recovery of protein and PS (approximately 50%), although the vaccine immunogenicity was maintained. Using gamma irradiation on freeze-dried sample, it was possible to maintain the integrity of OMV structure and, consequently, its ability to induce bactericidal antibodies.