RESUMEN
Lichens are a symbiosis between a fungus and one or more photosynthetic microorganisms that enables the symbionts to thrive in places and conditions they could not compete independently. Exchanges of water and sugars between the symbionts are the established mechanisms that support lichen symbiosis. Herein, we present a new linkage between algal photosynthesis and fungal respiration in lichen Flavoparmelia caperata that extends the physiological nature of symbiotic co-dependent metabolisms, mutually boosting energy conversion rates in both symbionts. Measurements of electron transport by oximetry show that photosynthetic O2 is consumed internally by fungal respiration. At low light intensity, very low levels of O2 are released, while photosynthetic electron transport from water oxidation is normal as shown by intrinsic chlorophyll variable fluorescence yield (period-4 oscillations in flash-induced Fv/Fm). The rate of algal O2 production increases following consecutive series of illumination periods, at low and with limited saturation at high light intensities, in contrast to light saturation in free-living algae. We attribute this effect to arise from the availability of more CO2 produced by fungal respiration of photosynthetically generated sugars. We conclude that the lichen symbionts are metabolically coupled by energy conversion through exchange of terminal electron donors and acceptors used in both photosynthesis and fungal respiration. Algal sugars and O2 are consumed by the fungal symbiont, while fungal delivered CO2 is consumed by the alga.
Asunto(s)
Dióxido de Carbono/metabolismo , Líquenes/metabolismo , Oxígeno/metabolismo , Fotosíntesis , Simbiosis , Aerobiosis/efectos de la radiación , Anaerobiosis/efectos de la radiación , Respiración de la Célula/efectos de la radiación , Clorofila/metabolismo , Electrodos , Transporte de Electrón/efectos de la radiación , Fluorescencia , Luz , Oxidación-Reducción , Fotosíntesis/efectos de la radiación , Simbiosis/efectos de la radiación , Factores de Tiempo , Agua/metabolismoRESUMEN
Antibiotic fermentation residues is a hazardous waste due to the existence of residual antibiotics and antibiotic resistance genes (ARGs), probably leading to the induction and spread of antibiotic resistant bacteria (ARB) in the environment, which could pose potential harm to the ecosystem and human health. It is urgent to develop an effective technology to remove the residual antibiotics and ARGs. In this study, the anaerobic digestion combined with gamma irradiation was applied for the disposal and utilization of cephalosporin C fermentation residues. The experimental results showed that the antibacterial activities of cephalosporin C against Staphylococcus aureus were significantly decreased after anaerobic digestion. The removal of tolC, a multidrug resistant gene, was improved up to 100% by the combination of gamma irradiation and anaerobic digestion compared to solely anaerobic digestion process, which may be due to the changes of microbial community structures induced by gamma irradiation.
Asunto(s)
Anaerobiosis/efectos de la radiación , Antibacterianos/química , Biodegradación Ambiental/efectos de la radiación , Cefalosporinas/química , Rayos gamma , Proteínas de la Membrana Bacteriana Externa/genética , Residuos de Medicamentos , Farmacorresistencia Bacteriana , Proteínas de Escherichia coli/genética , Fermentación , Cinética , Proteínas de Transporte de Membrana/genética , Staphylococcus aureus/efectos de los fármacos , Staphylococcus aureus/genética , Eliminación de Residuos LíquidosRESUMEN
Low oxygen conditions occur in grass sites due to high and frequent precipitation, poor soil quality, and over-irrigation followed by slow drainage. Three warm-season and one cool-season grass were analyzed at metabolic level during a time-course experiment performed in a controlled anoxic environment. Prolonged oxygen depletion proved detrimental by leading to premature death to all the species, with the exception of seashore paspalum. Moreover, the anoxia tolerance observed in these grasses has been associated with slow use of carbohydrates, rather than with their relative abundance, which was more important than their antioxidant capacity. Further physiological characterization of eight seashore paspalum genotypes to anoxia was also performed, by examining the variation in photosystem II (PSII) efficiency and gas exchange during post-anoxia recovery. Multivariate analysis highlighted the presence of three main clusters of seashore paspalum genotypes, characterized by different ability to restore the PSII photochemistry during recovery after one day of anoxia. Taken together, our data demonstrate that the analysis of post-anoxia recovery of fluorescence and gas exchange parameters can represent a fast and reliable indicator for selecting species and cultivars more able to acclimate their photosynthetic apparatus.
Asunto(s)
Oxígeno/metabolismo , Fotosíntesis , Poaceae/fisiología , Carácter Cuantitativo Heredable , Adaptación Fisiológica/efectos de la radiación , Alcohol Deshidrogenasa/metabolismo , Anaerobiosis/efectos de la radiación , Análisis Factorial , Genotipo , Luz , Fotosíntesis/efectos de la radiación , Complejo de Proteína del Fotosistema II/metabolismo , Poaceae/enzimología , Poaceae/genética , Poaceae/efectos de la radiación , Estaciones del Año , Solubilidad , Especificidad de la Especie , Azúcares/metabolismoRESUMEN
Huge amount of aging biogas slurry is in urgent need to be treated properly. However, due to high NH3-N concentration and low C/N ratio, this aging biogas slurry is refractory for traditional methods. Its denitrification has become a big challenge. In this paper, photosynthetic bacteria (PSB) were employed to handle this problem. The results showed denitrification of aging biogas slurry by PSB treatment was promising. The highest removal efficiency of NH3-N reached 99.75%, much higher than all other treatments. The removal of NH3-N followed pseudo zero order reaction under dark-aerobic condition. The better inoculation rate for NH3-N removal was 30%; and aerobic condition was more beneficial for NH3-N removal than anaerobic condition because of different metabolic pathways.
Asunto(s)
Bacterias/metabolismo , Biocombustibles/microbiología , Desnitrificación , Granjas , Ganado , Fotosíntesis , Amoníaco/aislamiento & purificación , Anaerobiosis/efectos de los fármacos , Anaerobiosis/efectos de la radiación , Animales , Desnitrificación/efectos de los fármacos , Desnitrificación/efectos de la radiación , Estudios de Factibilidad , Concentración de Iones de Hidrógeno , Cinética , Luz , Oxígeno/farmacología , Fotosíntesis/efectos de los fármacos , Fotosíntesis/efectos de la radiaciónRESUMEN
Anoxygenic phototrophic bacteria are a diverse collection of organisms that are defined by their ability to grow using energy from light without evolving oxygen. The dominant groups are purple sulfur bacteria, purple nonsulfur bacteria, green sulfur bacteria, and green and red filamentous anoxygenic phototrophic bacteria. They represent several bacterial phyla but they all have bacteriochlorophylls and carotenoids and photochemical reaction centers which generate ATP and cellular reductants used for CO2 fixation. They typically have an anaerobic lifestyle in the light, although some grow aerobically in the dark. Some of them oxidize inorganic sulfur compounds for light-dependent CO2 fixation; this ability can be exploited for photobiological removal of hydrogen sulfide from wastewater and biogas. The anoxygenic phototrophic bacteria also perform bioremediation of recalcitrant dyes, pesticides, and heavy metals under anaerobic conditions. Finally, these organisms may be useful for overexpression of membrane proteins and photobiological production of H2 and other valuable compounds.
Asunto(s)
Bacterias Anaerobias/metabolismo , Proteínas Bacterianas/metabolismo , Contaminantes Ambientales/metabolismo , Fotosíntesis/fisiología , Administración de Residuos/métodos , Anaerobiosis/fisiología , Anaerobiosis/efectos de la radiación , Bacterias Anaerobias/efectos de la radiación , Biodegradación Ambiental , Biotecnología/tendencias , Dióxido de Carbono/metabolismo , Contaminantes Ambientales/aislamiento & purificación , Luz , Modelos Biológicos , Fotosíntesis/efectos de la radiaciónRESUMEN
Comparative analyses of bacterial and archaeal community structures and dynamics in three biogas digesters during start-up and subsequent operation using microwaved, ultrasonicated or untreated waste activated sludge were performed based on 454 pyrosequencing datasets of part of 16S ribosomal RNA sequences and quantitative PCR. The pre-treatment increased the solubility, and thus the availability of the substrate for microbial degradation and significantly affected the succession of the anaerobic community structure over the course of the digestion. Bacteroidetes, Proteobacteria and Firmicutes were the dominant phyla in all digesters throughout operation. Proteobacteria decreased in relative abundance from 23-26 % to 11-13 % in association with enhanced substrate availability. Negative correlations between relative abundance of Alpha-, Beta- and Gammaproteobacteria and the substrate availability and/or biogas production were disclosed in statistical analyses. Clostridiales was the dominant order in Firmicutes, and Clostridiales, Clostridia and Firmicutes relative abundance and richness were shown to positively correlate with substrate availability and biogas generation. Methanogenic communities had a fairly restricted structure, highly dominated by Methanosaeta and Methanobrevibacter phylotypes. A gradual decline in Methanobrevibacter and increased representation of Methanosaeta concilii over time were particularly apparent in the digester receiving untreated waste activated sludge, whereas more diversified archaeal communities were maintained in the pre-treatment digesters. The quantitative PCR analyses revealed a methanogenic community distribution that coincided with the 454 pyrosequencing data.
Asunto(s)
Metano/biosíntesis , Consorcios Microbianos/fisiología , Microondas , Aguas del Alcantarillado/microbiología , Ondas Ultrasónicas , Anaerobiosis/fisiología , Anaerobiosis/efectos de la radiación , Archaea/genética , Archaea/fisiología , Archaea/efectos de la radiación , Bacteroidetes/genética , Bacteroidetes/fisiología , Bacteroidetes/efectos de la radiación , Biocombustibles , Reactores Biológicos/microbiología , Euryarchaeota/genética , Euryarchaeota/fisiología , Euryarchaeota/efectos de la radiación , Variación Genética , Secuenciación de Nucleótidos de Alto Rendimiento , Consorcios Microbianos/genética , Consorcios Microbianos/efectos de la radiación , Proteobacteria/genética , Proteobacteria/fisiología , Proteobacteria/efectos de la radiación , ARN Ribosómico 16S , Reacción en Cadena en Tiempo Real de la Polimerasa , Aguas del Alcantarillado/químicaRESUMEN
BACKGROUND: Oxygen is generally considered essential for lethal photosensitisation by photodynamic processes. The oral anaerobes, Prevotella intermedia and P. nigrescens are known to be photosensitive, but are also extremely sensitive to the cytotoxic effects of oxygen. METHODS: The Prevotellaceae were exposed to two 405 nm light sources for different exposure times in an anaerobic chamber. Viable counts of the light exposed samples were compared to light-free controls to determine the proportion of bacteria killed. RESULTS: Lethal photosensitivity was demonstrated against P. intermedia and P. nigrescens. The proportions of bacteria killed by either the light-emitting diode or laser pointer were similar at a given energy density (J/cm(2)). CONCLUSIONS: Lethal photosensitivity was demonstrated in two species of Prevotella under anaerobic conditions.
Asunto(s)
Apoptosis/fisiología , Oxígeno/metabolismo , Porfirinas/metabolismo , Prevotella/fisiología , Prevotella/efectos de la radiación , Tolerancia a Radiación/fisiología , Anaerobiosis/fisiología , Anaerobiosis/efectos de la radiación , Apoptosis/efectos de la radiación , Relación Dosis-Respuesta en la Radiación , Luz , Prevotella/clasificación , Dosis de Radiación , Especificidad de la EspecieRESUMEN
The model green microalga Chlamydomonas reinhardtii is frequently subject to periods of dark and anoxia in its natural environment. Here, by resorting to mutants defective in the maturation of the chloroplastic oxygen-sensitive hydrogenases or in Proton-Gradient Regulation-Like1 (PGRL1)-dependent cyclic electron flow around photosystem I (PSI-CEF), we demonstrate the sequential contribution of these alternative electron flows (AEFs) in the reactivation of photosynthetic carbon fixation during a shift from dark anoxia to light. At light onset, hydrogenase activity sustains a linear electron flow from photosystem II, which is followed by a transient PSI-CEF in the wild type. By promoting ATP synthesis without net generation of photosynthetic reductants, the two AEF are critical for restoration of the capacity for carbon dioxide fixation in the light. Our data also suggest that the decrease in hydrogen evolution with time of illumination might be due to competition for reduced ferredoxins between ferredoxin-NADP(+) oxidoreductase and hydrogenases, rather than due to the sensitivity of hydrogenase activity to oxygen. Finally, the absence of the two alternative pathways in a double mutant pgrl1 hydrogenase maturation factor G-2 is detrimental for photosynthesis and growth and cannot be compensated by any other AEF or anoxic metabolic responses. This highlights the role of hydrogenase activity and PSI-CEF in the ecological success of microalgae in low-oxygen environments.
Asunto(s)
Ciclo del Carbono , Chlamydomonas reinhardtii/fisiología , Hidrogenasas/metabolismo , Fotosíntesis , Proteínas de Plantas/metabolismo , Protones , Anaerobiosis/efectos de la radiación , Ciclo del Carbono/efectos de la radiación , Supervivencia Celular/efectos de la radiación , Chlamydomonas reinhardtii/citología , Chlamydomonas reinhardtii/crecimiento & desarrollo , Chlamydomonas reinhardtii/efectos de la radiación , Transporte de Electrón/efectos de la radiación , Ferredoxina-NADP Reductasa/metabolismo , Hidrógeno/metabolismo , Luz , Modelos Biológicos , Fotosíntesis/efectos de la radiación , Complejo de Proteína del Fotosistema I/metabolismo , Complejo de Proteína del Fotosistema II/metabolismo , Almidón/metabolismoRESUMEN
The effects of simulated outdoor seasonal climatic conditions on Nannochloropsis salina (N. salina) grown using nutrients from anaerobic digestion (AD) effluent were evaluated in this study. Under various light exposure (LE) and temperature (10-30 °C) conditions, N. salina specific growth rate (µ) was strongly affected by LE. Light availability (LA) was observed to be crucial for biomass production, with µ values of 0.038±0.013 d(-1), 0.093±0.013 d(-1), and 0.151±0.021 d(-1) for 6-h, 12-h, and 24-h LA conditions, respectively. Temperature (10-25 °C) was not significant in affecting the light dependent growth coefficient (µ/LE), indicating the suitability of culturing this strain in the Ohio climate. Cultures exposed to low illumination had significantly higher unsaturated fatty acid content than those under high illumination, with nearly 29% higher eicosapentaenoic acid (C20:5) content. Using LE and light attenuation resulted in adequate prediction of N. salina growth in a 1000 L open raceway pond.
Asunto(s)
Microalgas/crecimiento & desarrollo , Nitrógeno/farmacología , Fósforo/farmacología , Agua de Mar/microbiología , Eliminación de Residuos Líquidos , Anaerobiosis/efectos de los fármacos , Anaerobiosis/efectos de la radiación , Reactores Biológicos/microbiología , Simulación por Computador , Ácidos Grasos/biosíntesis , Cinética , Luz , Microalgas/efectos de los fármacos , Microalgas/efectos de la radiación , Modelos Teóricos , Nitrógeno/análisis , Fósforo/análisis , Estanques , Reproducibilidad de los Resultados , Estaciones del Año , TemperaturaRESUMEN
Several batch culture studies were carried out to evaluate an anaerobically treated effluent as a low-cost growth medium for the microalga Chlamydomonas acidophila and to study the effectiveness of the microalga in removing NH4-N from the effluent. An initial decrease in the effluent pH to 3 was required for adequate growth of C. acidophila and removal of NH4-N. Growth of the microalgae was inhibited at high light intensity (224µmolphotonsm(-2)s(-1) at the surface of the vessels). However, the growth was not greatly affected by the high solid content and turbidity of the effluent. The microalga was able to grow in media containing NH4-N at concentrations of up to 1000mgL(-1) (50% of effluent) and to remove 88mg of NH4-NL(-1) in 10days. C. acidophila therefore appears a promising agent for the removal of NH4-N from anaerobically treated effluents.
Asunto(s)
Compuestos de Amonio/aislamiento & purificación , Chlamydomonas/metabolismo , Eliminación de Residuos Líquidos , Anaerobiosis/efectos de los fármacos , Anaerobiosis/efectos de la radiación , Biodegradación Ambiental/efectos de los fármacos , Chlamydomonas/efectos de los fármacos , Chlamydomonas/crecimiento & desarrollo , Chlamydomonas/efectos de la radiación , Medios de Cultivo/farmacología , Concentración de Iones de Hidrógeno/efectos de los fármacos , Concentración de Iones de Hidrógeno/efectos de la radiación , Luz , Microalgas/efectos de los fármacos , Microalgas/crecimiento & desarrollo , Microalgas/efectos de la radiación , Nitrógeno/aislamiento & purificaciónRESUMEN
BACKGROUND: Lethal photosensitization has been previously demonstrated in Porphyromonas gingivalis, but oxygen is considered to be essential to this process. However, since P. gingivalis is a periodontal pathogen which grows in the low oxygen conditions found in the subgingival crevice, it was considered prudent to study its photosensitivity in anaerobic conditions. METHODS: A series of experiments were undertaken to attempt to induce lethal photosensitization in P. gingivalis (ATCC 33277) under strict anaerobic conditions using two different 405 nm light sources. Samples of P. gingivalis were grown on a blood-containing, solid growth medium before being suspended in saline and then exposed to 405 nm light delivered by either a hand-held light source (Toothcare™) (11.4 mW/cm(2)) or a laser pointer (328.5 mW/cm(2)). With the exception of the adjustment of the P. gingivalis suspensions to a fixed optical density, the experiments were carried out in their entirety within an anaerobic chamber. RESULTS: The lowest Toothcare light dose tested (0.34 J/cm(2); 30s) yielded a statically significant kill of 63.4% which increased to 94.1% kill at higher light doses (3.42 J/cm(2); 300 s). The laser pointer similarly achieved kills of 90.2% at the lower light dose tested (9.86 J/cm(2); 30s) and 94.5% kill at the highest light dose (98.55 J/cm(2); 300 s). CONCLUSIONS: Lethal photosensitization can be instigated in planktonic suspensions of P. gingivalis at 405 nm delivered by hand-held devices under anaerobic conditions. This suggests the possibility that lethal photosensitization occurred by the oxygen-independent type I pathway as oppose to the oxygen-dependent type II pathway.
Asunto(s)
Apoptosis/fisiología , Apoptosis/efectos de la radiación , Porfirinas/metabolismo , Porphyromonas gingivalis/fisiología , Porphyromonas gingivalis/efectos de la radiación , Anaerobiosis/fisiología , Anaerobiosis/efectos de la radiación , Relación Dosis-Respuesta en la Radiación , Luz , Oxígeno/metabolismo , Fármacos Fotosensibilizantes/metabolismo , Porphyromonas gingivalis/efectos de los fármacos , Dosis de RadiaciónRESUMEN
Microbial pathogens in municipal sewage sludges need to be inactivated prior to environmental disposal. The efficacy of high energy (10 MeV) e-beam irradiation to inactivate a variety of selected microbial pathogens and indicator organisms in aerobically and anaerobically digested sewage sludge was evaluated. Both bacterial and viral pathogens and indicator organisms are susceptible to e-beam irradiation. However, as expected there was a significant difference in their respective e-beam irradiation sensitivity. Somatic coliphages, bacterial endospores and enteric viruses were more resistant compared to bacterial pathogens. The current US EPA mandated 10 kGy minimum dose was capable of achieving significant reduction of both bacterial and viral pathogens. Somatic coliphages can be used as a microbial indicator for monitoring e-beam processes in terms of pathogen inactivation in sewage sludges.
Asunto(s)
Electrones , Viabilidad Microbiana/efectos de la radiación , Aguas del Alcantarillado/microbiología , Aguas del Alcantarillado/virología , Inactivación de Virus/efectos de la radiación , Aerobiosis/efectos de la radiación , Anaerobiosis/efectos de la radiación , Bacterias/efectos de la radiación , Esporas Bacterianas/efectos de la radiación , Virus/efectos de la radiaciónRESUMEN
Photolysis of some polycyclic aromatic hydrocarbons (PAHs) on soil surfaces may play an important role in the fate of PAHs in the environment. Photolysis of PAHs on soil surfaces under UV irradiation was investigated. The effects of oxygen, irradiation intensity and soil moisture on the degradation of the three PAHs were observed. The results showed that oxygen, soil moisture and irradiation intensity enhanced the photolysis of the three PAHs on soil surfaces. The degradation of the three PAHs on soil surfaces is related to their absorption spectra and the oxidation-half-wave potential. The photolysis of PAHs on soil surfaces in the presence of oxygen followed pseudo first-order kinetics. The photolysis half-lives ranged from 37.87 days for benzo[a]pyrene to 58.73 days for phenanthrene. The results indicate that photolysis is a successful way to remediate PAHs-contaminated soils.
Asunto(s)
Fotólisis/efectos de la radiación , Hidrocarburos Policíclicos Aromáticos/efectos de la radiación , Suelo/química , Rayos Ultravioleta , Anaerobiosis/efectos de la radiación , Humedad , Cinética , Oxígeno/química , Propiedades de SuperficieRESUMEN
The solid-state photo-CIDNP (photochemically induced dynamic nuclear polarization) effect is studied in photosynthetic reaction centers of Heliobacillus mobilis at different magnetic fields by (13)C MAS (magic-angle spinning) NMR spectroscopy. Two active states of heliobacterial reaction centers are probed: an anaerobic preparation of heliochromatophores ("Braunstoff", German for "brown substance") as well as a preparation of cells after exposure to oxygen ("Grünstoff", "green substance"). Braunstoff shows significant increase of enhanced absorptive (positive) signals toward lower magnetic fields, which is interpreted in terms of an enhanced differential relaxation (DR) mechanism. In Grünstoff, the signals remain emissive (negative) at two fields, confirming that the influence of the DR mechanism is comparably low.
Asunto(s)
Bacillus/metabolismo , Luz , Procesos Fotoquímicos/efectos de la radiación , Proteínas del Complejo del Centro de Reacción Fotosintética/metabolismo , Aerobiosis/efectos de la radiación , Anaerobiosis/efectos de la radiación , Bacterioclorofilas/química , Isótopos de Carbono , Espectroscopía de Resonancia Magnética , Quinonas/químicaRESUMEN
Electrogenic activity of oxygenic photo-bioelectrocatalytic fuel cell (PhFCOX) under mixotrophic mode was evaluated using atmospheric CO2 and domestic wastewater as carbon sources for harnessing bioelectricity with mixed microalgae as anodic biocatalyst. PhFCOX operation showed good electrogenic activity (3.55 µW/m(2)) associated with higher biomass growth (2.87 g/l) and chlorophyll content (5.12 mg/l). Electrogenic activity was relatively higher during the day time (46 mV; 0.6 mA) compared to the night (6 mV; 0.01 mA). Performance of PhFCOX undergoing oxygenic photosynthesis (DO; 3.5 mg/l) was compared with the mixotrophic fuel cell (PhFCAX) with photosynthetic bacteria as biocatalyst under anoxygenic conditions (DO; 0.45 mg/l). The dissolved oxygen produced during photolysis of water in oxygenic photosynthesis is a major limiting factor affecting the electrogenic activity. Voltammetric and amperometric analysis along with electron transfer kinetics (Tafel analysis) supported the bio-electrochemical behavior of PhFCOX and PhFCAX.
Asunto(s)
Fuentes de Energía Bioeléctrica/microbiología , Electricidad , Microalgas/metabolismo , Oxígeno/farmacología , Procesos Fotoquímicos/efectos de los fármacos , Anaerobiosis/efectos de los fármacos , Anaerobiosis/efectos de la radiación , Biomasa , Catálisis/efectos de los fármacos , Catálisis/efectos de la radiación , Ritmo Circadiano/efectos de los fármacos , Ritmo Circadiano/efectos de la radiación , Técnicas Electroquímicas , Luz , Microalgas/efectos de los fármacos , Microalgas/efectos de la radiación , Fotosíntesis/efectos de los fármacos , Fotosíntesis/efectos de la radiación , Solubilidad , Aguas Residuales/química , Purificación del AguaRESUMEN
Ag(2)O/TNBs were fabricated by depositing Ag(2)O nanoparticles on the surface of TiO(2) nanobelts (TNBs). The disinfection activities of Ag(2)O/TNBs on two representative bacterial types: Gram-negative Escherichia coli ATCC15597 and Gram-positive Bacillus subtilis, were examined under both dark and visible light conditions. Ag(2)O/TNBs exhibited stronger bactericidal activities than Ag(2)O nanoparticles and TNBs under both dark and light conditions. For both cell types, disinfection effects of Ag(2)O/TNBs were greater under light conditions relative to those under dark conditions. The bactericidal mechanisms of Ag(2)O/TNBs under both dark and light conditions were explored. Ag(+) ions released from Ag(2)O/TNBs did not contribute to the bactericidal activity of Ag(2)O/TNBs under dark conditions, whereas the released Ag(+) ions showed bactericidal activity under visible light irradiation conditions. Active species (H(2)O(2), O(2)(-)·, and e(-)) generated by Ag(2)O/TNBs played important roles in the disinfection processes under both dark and visible light irradiation conditions. Without the presence of active species, the direct contact of Ag(2)O/TNBs with bacterial cells had no bactericidal effect.
Asunto(s)
Antibacterianos/farmacología , Luz , Nanopartículas/química , Óxidos/farmacología , Compuestos de Plata/farmacología , Titanio/farmacología , Anaerobiosis/efectos de los fármacos , Anaerobiosis/efectos de la radiación , Bacillus subtilis/efectos de los fármacos , Bacillus subtilis/efectos de la radiación , Oscuridad , Desinfección , Escherichia coli/efectos de los fármacos , Escherichia coli/efectos de la radiación , Escherichia coli/ultraestructura , Peróxido de Hidrógeno/farmacología , Pruebas de Sensibilidad Microbiana , Nanopartículas/ultraestructuraRESUMEN
Perfluorooctanoic acid (PFOA) is a new-found hazardous persistent organic pollutant, and it is resistant to decomposition by hydroxyl radical (HO*) due to its stable chemical structure and the high electronegativity of fluorine. Photocatalytic reduction of PFOA with beta-Ga2O3 in anoxic aqueous solution was investigated for the first time, and the results showed that the photoinduced electron (e(cb-)) coming from the beta-Ga2O3 conduction band was the major degradation substance for PFOA, and shorter-chain perfluorinated carboxylic acids (PFCAs, CnF2n+i1COOH, 1 < or = n < or = 6) were the dominant products. Furthermore, the concentration of F- was measured by the IC technique and defluorination efficiency was calculated. After 3 hr, the photocatalytic degradation efficiency was 98.8% and defluorination efficiency was 31.6% in the presence of thiosulfate and bubbling N2. The degradation reaction followed first-order kinetics (k = 0.0239 min(-1), t1/2 = 0.48 hr). PFCAs (CnF2n+1COOH, 1 < or = n < or = 7) were detected and measured by LC-MS and LC-MS/MS methods. It was deduced that the probable photocatalytic degradation mechanism involves e(cb-) attacking the carboxyl of CnF2n+1COOH, resulting in decarboxylation and the generation of CnF2n+1*. The produced CnF2n+1* reacted with H2O, forming CnF2n+1OH, then CnF2n+1OH underwent HF loss and hydrolysis to form CnF2n+1COOH.
Asunto(s)
Caprilatos/química , Caprilatos/efectos de la radiación , Fluorocarburos/química , Fluorocarburos/efectos de la radiación , Galio/química , Fotólisis , Anaerobiosis/efectos de la radiación , Catálisis/efectos de la radiación , Halogenación/efectos de la radiación , Concentración de Iones de Hidrógeno/efectos de la radiación , Espectrometría de Masas , Oxidación-Reducción/efectos de la radiación , Fotólisis/efectos de la radiación , Soluciones , Factores de Tiempo , Rayos Ultravioleta , Difracción de Rayos XRESUMEN
Wheat (Triticum aestivum L.) seedlings contain four alanine aminotransferase (AlaAT) homologues. Two of them encode AlaAT enzymes, whereas two homologues act as glumate:glyoxylate aminotransferase (GGAT). To address the function of the distinct AlaAT homologues a comparative examination of the changes in transcript level together with the enzyme activity and alanine and glutamate content in wheat seedlings subjected to low oxygen availability, nitrogen and light deficiency has been studied. Shoots of wheat seedlings were more tolerant to hypoxia than the roots as judging on the basis of enzyme activity and transcript level. Hypoxia induced AlaAT1 earlier in roots than in shoots, while AlaAT2 and GGAT were unaffected. The increase in AlaAT activity lagged behind the increase in alanine content. Nitrogen deficiency has little effect on the activity of GGAT. In contrast, lower activity of AlaAT and the level of mRNA for AlaAT1 and AlaAT2 in wheat seedlings growing on a nitrogen-free medium seems to indicate that AlaAT is regulated by the availability of nitrogen. Both AlaAT and GGAT activities were present in etiolated wheat seedlings but their activity was half of that observed in light-grown seedlings. Exposure of etiolated seedlings to light caused an increase in enzyme activities and up-regulated GGAT1. It is proposed that hypoxia-induced AlaAT1 and light-induced peroxisomal GGAT1 appears to be crucial for the regulation of energy availability in plants grown under unfavourable environmental conditions. Key message In young wheat seedlings, both AlaAT and GGAT are down-regulated by nitrogen deficiency, whereas AlaAT1 is upregulated by hypoxia and GGAT1 by light.
Asunto(s)
Alanina Transaminasa/genética , Estrés Fisiológico/genética , Triticum/enzimología , Triticum/fisiología , Alanina/metabolismo , Alanina Transaminasa/metabolismo , Anaerobiosis/efectos de los fármacos , Anaerobiosis/genética , Anaerobiosis/efectos de la radiación , Secuencia de Bases , Cromatografía Líquida de Alta Presión , Cromatografía de Fase Inversa , Electroforesis en Gel de Poliacrilamida , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Regulación de la Expresión Génica de las Plantas/efectos de la radiación , Ácido Glutámico/metabolismo , Luz , Datos de Secuencia Molecular , Nitrógeno/farmacología , Filogenia , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/enzimología , Raíces de Plantas/genética , Raíces de Plantas/fisiología , Brotes de la Planta/efectos de los fármacos , Brotes de la Planta/enzimología , Brotes de la Planta/genética , Brotes de la Planta/fisiología , ARN Mensajero/genética , ARN Mensajero/metabolismo , Plantones/efectos de los fármacos , Plantones/enzimología , Plantones/genética , Plantones/efectos de la radiación , Homología de Secuencia de Ácido Nucleico , Estrés Fisiológico/efectos de los fármacos , Estrés Fisiológico/efectos de la radiación , Transaminasas/genética , Transaminasas/metabolismo , Triticum/efectos de los fármacos , Triticum/genéticaRESUMEN
Electrogenic activity of photo-bioelectrocatalytic /photo-biological fuel cell (PhFC) was evaluated in a mixotrophic mode under anoxygenic microenvironment using photosynthetic consortia as biocatalyst. An acetate rich wastewater was used as anolyte for harnessing energy along with additional treatment. Mixotrophic operation facilitated good electrogenic activity and wastewater treatment associated with biomass growth. PhFC operation documented feasible microenvironment for the growth of photosynthetic bacteria compared to algae which was supported by pigment (total chlorophyll and bacteriochlorophyll) and diversity analysis. Pigment data also illustrated the association between bacterial and algal species. The synergistic interaction between anoxygenic and oxygenic photosynthesis was found to be suitable for PhFC operation. Light dependent deposition of electrons at electrode was relatively higher compared to dark dependent electron deposition under anoxygenic condition. PhFC documented for good volatile fatty acids removal by utilizing them as electron donor. Bioelectrochemical behavior of PhFC was evaluated by voltammetric and chronoamperometry analysis.
Asunto(s)
Bacterias/metabolismo , Fuentes de Energía Bioeléctrica/microbiología , Biotecnología/instrumentación , Biotecnología/métodos , Electricidad , Luz , Fotobiorreactores/microbiología , Acetatos/análisis , Anaerobiosis/efectos de los fármacos , Anaerobiosis/efectos de la radiación , Bacterias/efectos de los fármacos , Bacterioclorofilas/metabolismo , Biodegradación Ambiental/efectos de los fármacos , Biodiversidad , Análisis de la Demanda Biológica de Oxígeno , Catálisis/efectos de los fármacos , Catálisis/efectos de la radiación , Ritmo Circadiano/efectos de los fármacos , Ritmo Circadiano/efectos de la radiación , Conservación de los Recursos Energéticos , Técnicas Electroquímicas , Electrodos , Ácidos Grasos Volátiles/análisis , Oxígeno/farmacología , Fotosíntesis/efectos de los fármacos , Fotosíntesis/efectos de la radiación , Solubilidad/efectos de los fármacos , Solubilidad/efectos de la radiación , Eliminación de Residuos Líquidos , Purificación del AguaRESUMEN
The effect of substrate (glucose) concentration on the stability and yield of a continuous fermentative process that produces hydrogen was studied. Four anaerobic fluidized bed reactors (AFBRs) were operated with a hydraulic retention time (HRT) from 1 to 8 h and an influent glucose concentration from 2 to 25 g L(-1). The reactors were inoculated with thermally pre-treated anaerobic sludge and operated at a temperature of 30 °C with an influent pH around 5.5 and an effluent pH of about 3.5. The AFBRs with a HRT of 2 h and a feed strength of 2, 4, and 10 g L(-1) showed satisfactory H(2) production performance, but the reactor fed with 25 g L(-1) of glucose did not. The highest hydrogen yield value was obtained in the reactor with a glucose concentration of 2 g L(-1) when it was operated at a HRT of 2 h. The maximum hydrogen production rate value was achieved in the reactor with a HRT of 1 h and a feed strength of 10 g L(-1). The AFBRs operated with glucose concentrations of 2 and 4 g L(-1) produced greater amounts of acetic and butyric acids, while AFBRs with higher glucose concentrations produced a greater amount of solvents.