RESUMEN
Cresyl violet and cresyl red, components of commercial cresyl violet acetate, were separated and purified using preparative column liquid chromatography. The stationary phase was silica gel and gradient elution was carried out using chloroform:methanol. The purified dyes were obtained in high yield; 51% of the original lot was recovered as cresyl violet and 40% as cresyl red. Separated materials were characterized by nuclear magnetic resonance and mass spectroscopy; UV-visible and Fourier-transform infrared spectra also were obtained for samples of pure cresyl violet and cresyl red. The colored constituents of the commercial dye lot were identified using thin layer chromatography and reverse phase high performance liquid chromatography. Both methodologies were suitable for routine testing; reverse phase high performance liquid chromatography is an appropriate tool for quality control and high resolution identification of these compounds.
Asunto(s)
Benzoxazinas/química , Colorantes/química , Benzoxazinas/aislamiento & purificación , Cromatografía Líquida de Alta Presión , Cromatografía en Capa Delgada , Colorantes/aislamiento & purificación , Indicadores y Reactivos , Espectroscopía de Resonancia Magnética , Espectrometría de Masa por Ionización de Electrospray , Espectrofotometría Ultravioleta , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
Secondary metabolites, DIBOA, HBOA, 7-OH-HBOA, BOA and gallic acid, were isolated and quantified from Calceolaria thyrsiflora Graham, a native medicinal plant of Chile belonging to the Scrophulariaceae family. The highest DIBOA contents were determined in leaves (145 mmol kg(-1) dry wt) and flowers (161 mmol kg(-1) dry wt). Antibacterial activities of DIBOA, HBOA, BOA, gallic acid and infusions of flowers and leaves were determined. The phytomedicinal properties attributed to C. thyrsiflora Graham could be understood on the basis of its antibacterial activity.