RESUMEN
Background: Nicotinamide phosphoribosyltransferase (NAMPT) catalyzes the rate-limiting step in the salvage pathway of mammalian nicotinamide adenine dinucleotide (NAD+) biosynthesis. Through its NAD+-biosynthetic activity, NAMPT is able to regulate the development of hepatic steatosis and inflammation induced by diet or alcohol. However, the roles NAMPT plays in the development of liver fibrosis remain obscure. Purpose: To investigate the roles of NAMPT-mediated NAD+ biosynthesis in hepatic stellate cell (HSC) activation and liver fibrosis. Research Design: Realtime RT-PCR and western blot analyses were performed to analyze the expression of profibrogenic genes. Sirius red staining was conducted to examine the fibrosis in liver. Mouse liver fibrosis was induced by intraperitoneal injection of carbon tetrachloride (CCl4) 2 times a week for 6 weeks. Adenovirus-mediated NAMPT overexpression or nicotinamide mononucleotide (NMN) administration was carried out to study the effects of elevation of NAD+ levels on protecting CCl4-induced liver fibrosis in mice. LX2 cells or primary HSCs were used to study the role of NAMPT overexpression or NMN treatment in reducing profibrogenic gene expression in vitro. ResultsCCl4 administration suppresses NAMPT expression in liver and reduces hepatic NAD+ content. Tgfß1 treatment decreases intracellular NAD+ levels and NAMPT expression in LX2 cells. Adenovirus-mediated NAMPT overexpression augments liver NAD+ levels, inhibits HSC activation and alleviates CCl4-induced liver fibrosis in mice. Administration of NMN also suppresses HSC activation and protects against CCl4-induced liver fibrosis in mice. Conclusions: NAMPT-mediated NAD+ biosynthesis inhibits HSC activation and protects against CCl4-induced liver fibrosis.
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Intoxicación por Tetracloruro de Carbono/complicaciones , Células Estrelladas Hepáticas/metabolismo , Cirrosis Hepática/etiología , NAD/biosíntesis , Nicotinamida Fosforribosiltransferasa/metabolismo , Animales , Tetracloruro de Carbono/toxicidad , Intoxicación por Tetracloruro de Carbono/prevención & control , Ratones , Ratones Endogámicos C57BLRESUMEN
INTRODUCTION AND OBJECTIVES: Cirrhosis has gradually become a serious public health issue, especially the national prevalence of cirrhosis was 29.2% in northwest China. Recent evidence has revealed that intestinal barrier (IB) dysfunction results from and contributes to cirrhosis. Our previous results have indicated that insulin-like growth factors (IGF-1) improved the impaired IB function and downregulated high mobility group protein box-1 (HMGB-1). Nevertheless, the role of the IGF-1/HMGB1 axis in cirrhosis remains largely unknown. MATERIALS AND METHODS: Western blotting and qRT-PCR were used to detect protein and mRNA levels of related genes. The levels of AST, ALT, IL-1ß, and TNF-α were examined using commercial kits. Immunofluorescence was used to evaluate the expression of HMGB1 in tissues. RESULTS: In carbon tetrachloride (CCl4)-treated rat, the levels of AST (380.12 vs. 183.97), ALT (148.12 vs. 53.56), IL-1ß (155.94 vs. 55.60), and TNF-α (155.00 vs. 48.90) were significantly increased compared with the control group, while IGF-1 treatment significantly alleviated CCL4-induced inflammatory response and IB dysfunction by downregulating HMGB1-mediated the TLR4/MyD88/NF-κB signaling pathway. In vitro experiments, HMGB1 treatment promoted inflammatory cytokines secretion and reduced cell viability and tight junctions by activating the TLR4/MyD88/NF-κB signaling pathway in Caco-2 cells, but IGF-1 alleviated these effects. CONCLUSION: Our findings suggest that IGF-1 might serve as a potential therapeutic target for cirrhosis and IB dysfunction via inactivation of the TLR4/MyD88/NF-κB pathway through down-regulation HMGB1.
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Intoxicación por Tetracloruro de Carbono/complicaciones , Regulación hacia Abajo , Regulación de la Expresión Génica , Proteína HMGB1/genética , Factor I del Crecimiento Similar a la Insulina/uso terapéutico , Mucosa Intestinal/metabolismo , Cirrosis Hepática Experimental/genética , Animales , Células CACO-2 , Intoxicación por Tetracloruro de Carbono/genética , Intoxicación por Tetracloruro de Carbono/metabolismo , Proteína HMGB1/biosíntesis , Humanos , Mucosa Intestinal/patología , Cirrosis Hepática Experimental/inducido químicamente , Cirrosis Hepática Experimental/terapia , Masculino , ARN/genética , RatasRESUMEN
OBJECTIVES: Spirulina platensis (SP) is an edible Cyanobacterium with ethnomedicinal significance. This study aims at evaluating the beneficial effect of SP against carbon tetrachloride (CCl4)-induced liver toxicity in male rats. METHODS: Rats received intraperitoneal injections of CCl4 (2 ml/kg body weight [b.w.] per every other day) for 40 days, alone or in combination with oral treatments of SP (400 mg/kg b.w. per day). KEY FINDINGS: SP attenuated haematological disturbances, serum liver markers, hepatic necrosis and inflammation, and dyslipidemia in CCl4-intoxicated rats. SP also reduced CCl4-induced oxidative stress by increasing the activities of antioxidant enzymes, such as glutathione peroxidase, superoxide dismutase and catalase and glutathione content, and inhibiting lipid peroxidation products and nitric oxide levels in the rat liver. Further investigations revealed that SP counteracted CCl4-induced increased hepatic levels of Ki-67 (a parameter of cell proliferation), interleukin-6, and tumour necrosis factor-alpha and cyclooxygenase-2 messenger RNA expression. Noticeably, the supplementation of SP restored the decrease of proapoptotic p53 protein levels in the liver of rats treated with CCl4. CONCLUSIONS: SP prevented liver damage in CCl4-treated rats via augmentation of antioxidant defense mechanisms and inhibition of inflammatory cytokines/mediators and antiproliferative effects.
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Antioxidantes/uso terapéutico , Tetracloruro de Carbono/toxicidad , Enfermedad Hepática Inducida por Sustancias y Drogas/prevención & control , Hígado/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Fitoterapia , Spirulina , Animales , Antioxidantes/metabolismo , Antioxidantes/farmacología , Apoptosis , Intoxicación por Tetracloruro de Carbono/complicaciones , Intoxicación por Tetracloruro de Carbono/metabolismo , Intoxicación por Tetracloruro de Carbono/prevención & control , Enfermedad Hepática Inducida por Sustancias y Drogas/complicaciones , Enfermedad Hepática Inducida por Sustancias y Drogas/metabolismo , Citocinas/metabolismo , Suplementos Dietéticos , Dislipidemias/prevención & control , Glutatión/metabolismo , Glutatión Peroxidasa/metabolismo , Inflamación/metabolismo , Inflamación/prevención & control , Peroxidación de Lípido , Hígado/metabolismo , Masculino , Óxido Nítrico/metabolismo , Extractos Vegetales/farmacología , Extractos Vegetales/uso terapéutico , Ratas Wistar , Superóxido Dismutasa/metabolismo , Proteína p53 Supresora de Tumor/metabolismoAsunto(s)
Intoxicación por Tetracloruro de Carbono/complicaciones , Tetracloruro de Carbono/toxicidad , Adulto , Tetracloruro de Carbono/efectos adversos , Tetracloruro de Carbono/historia , Intoxicación por Tetracloruro de Carbono/etiología , Servicio de Urgencia en Hospital/organización & administración , Sistemas de Extinción de Incendios/historia , Hematemesis/etiología , Historia del Siglo XX , Humanos , Hígado/anomalías , Hígado/efectos de los fármacos , Hígado/fisiopatología , Masculino , Vómitos/tratamiento farmacológico , Vómitos/etiologíaRESUMEN
Acute liver injury can be caused by chemicals and can lead to liver failure. We investigated the protective effect of helicid (HEL) on acute liver injury caused by CCl4 in mice. We found that ALT and AST levels as well as hepatic pathological damage in mice treated with CCl4 was increased significantly, while the effects were decreased by HEL treatment. HEL treatment increased the activity of T-SOD, GSH and CAT and reduced the level of MDA in CCl4 treated mice. HEL improved the histopathology of liver caused by CCl4. HEL also reduced TNF-α, IL-1ß and IL- 6 activity caused by CCl4. We investigated the phosphorylation of p65 and IκB protein and found that HEL can alleviate liver damage via the NF-κB signaling pathway. Our findings indicate that HEL protects against acute liver injury induced by CCl4. The protective effect of HEL appears to be due to its antioxidative and anti-inflammatory properties through the NF-κB signaling pathway.
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Antiinflamatorios/farmacología , Antioxidantes/farmacología , Benzaldehídos/uso terapéutico , Intoxicación por Tetracloruro de Carbono/complicaciones , Enfermedad Hepática Inducida por Sustancias y Drogas/prevención & control , Alanina Transaminasa/metabolismo , Animales , Antiinflamatorios/química , Antioxidantes/química , Aspartato Aminotransferasas/metabolismo , Benzaldehídos/farmacología , Regulación de la Expresión Génica/efectos de los fármacos , Inflamación/inducido químicamente , Inflamación/tratamiento farmacológico , Hígado/efectos de los fármacos , Hígado/patología , Masculino , Ratones , Ratones Endogámicos ICR , FN-kappa B/genética , FN-kappa B/metabolismoAsunto(s)
Lesión Renal Aguda/inducido químicamente , Lesión Renal Aguda/terapia , Intoxicación por Tetracloruro de Carbono/metabolismo , Janus Quinasa 2/metabolismo , Factor de Transcripción STAT3/metabolismo , Transducción de Señal , Factor de Transcripción ReIA/metabolismo , Lesión Renal Aguda/enzimología , Lesión Renal Aguda/metabolismo , Animales , Intoxicación por Tetracloruro de Carbono/complicaciones , Modelos Animales de Enfermedad , Hidrógeno/metabolismo , Mediadores de Inflamación/antagonistas & inhibidores , Mediadores de Inflamación/metabolismo , Masculino , Malondialdehído/metabolismo , Ratones , Estrés OxidativoRESUMEN
Occupational acute carbon tetrachloride poisoning occurs when a worker is exposed to highly condensed carbon tetrachloride in a short time and suffers from diseases damaging mainly his neutral system and (or) liver and kidney. This article reports a case of what clinical symptoms MODS patients show and how to diagnose, differential diagnosis and therapy process.
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Intoxicación por Tetracloruro de Carbono/complicaciones , Insuficiencia Multiorgánica/diagnóstico , Enfermedades Profesionales/diagnóstico , Humanos , Insuficiencia Multiorgánica/inducido químicamente , Enfermedades Profesionales/inducido químicamenteRESUMEN
AIM: To investigate whether fecal microbiota transplantation (FMT) prevents hepatic encephalopathy (HE) in rats with carbon tetrachloride (CCl4)-induced acute hepatic dysfunction. METHODS: A rat model of HE was established with CCl4. Rat behaviors and spatial learning capability were observed, and hepatic necrosis, intestinal mucosal barrier, serum ammonia levels and intestinal permeability were determined in HE rats receiving FMT treatment. Furthermore, the expression of tight junction proteins (Claudin-1, Claudin-6 and Occludin), Toll-like receptor (TLR) 4/TLR9, interleukin (IL)-1ß, IL-6 and tumor necrosis factor (TNF)-α was examined. RESULTS: FMT improved rat behaviors, HE grade and spatial learning capability. Moreover, FMT prevented hepatic necrosis and intestinal mucosal barrier damage, leading to hepatic clearance of serum ammonia levels and reduced intestinal permeability. The expression of TLR4 and TLR9, two potent mediators of inflammatory response, was significantly downregulated in the liver of rats treated with FMT. Consistently, circulating pro-inflammatory factors such as interleukin (IL)-1ß, IL-6 and tumor necrosis factor-α were remarkably decreased, indicating that FMT is able to limit systemic inflammation by decreasing the expression of TLR4 and TLR9. Importantly, HE-induced loss of tight junction proteins (Claudin-1, Claudin-6 and Occludin) was restored in intestinal tissues of rats receiving FMT treatment. CONCLUSION: FMT enables protective effects in HE rats, and it improves the cognitive function and reduces the liver function indexes. FMT may cure HE by altering the intestinal permeability and improving the TLR response of the liver.
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Trasplante de Microbiota Fecal , Encefalopatía Hepática/prevención & control , Amoníaco/sangre , Animales , Intoxicación por Tetracloruro de Carbono/complicaciones , Encefalopatía Hepática/sangre , Encefalopatía Hepática/etiología , Mucosa Intestinal/metabolismo , Masculino , Memoria , Ratas Sprague-DawleyRESUMEN
In spite of the tremendous stride in modern medicine, conventional drugs used in the hepatotoxic management are mostly inadequate. The present study aims in the synthesis of novel Schiff base compound derived using s-allyl cystiene and methionine. The newly synthesized compound, 2-((2-((2-(allylthio)-1-carboxyethyl)imino)ethylidene)amino)-4-(methylthio)butanoic acid (ACEMB) was characterized using UV-visible spectrophotometer, FTIR, 1HNMR, and GC-MS. ACEMB showed potent in vitro antioxidant property. Chronic administration of ACEMB prior to CCl4 intoxication: i) attenuated the leakage of liver injury markers, such as, enzymes (AST, ALT, GGT, ALP and LDH) and biomolecules (bilirubin) into the blood circulation; ii) normalized the concentration of total proteins, albumin and globulin to control level; and iii) protected the liver against dyslipidemia. These effects of ACEMB show the preservation of endoplasmic reticulum function against CCl4 toxicity in the liver. The protective effect of ACEMB was due to its antioxidant property, which was revealed by reduced oxidative stress (TBARS and HP) and enhanced functions of the endogenous antioxidative system (SOD, catalase, GPx, GST, GSH, vitamin E and C) against CCl4 intoxication. Also, ACEMB protected the functional activities of the various mitochondrial tricarboxylic acid cycle and oxidative phosphorylation enzymes. The biochemical alterations are in concurrence with the histological observations, wherein ACEMB pretreatment prevented the vacuolation, degeneration of nuclei and necrosis of hepatocytes. In addition, in silico analysis reveals the interaction of ACEMB in the active site of cytochrome P450. ACEMB mediates hepatoprotective effect by substituting itself as an antioxidant and decreasing oxidative stress, thereby diminishing the intracellular organelle dysfunction against CCl4 toxicity in the liver.
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Antioxidantes/uso terapéutico , Intoxicación por Tetracloruro de Carbono/complicaciones , Cisteína/análogos & derivados , Iminas/uso terapéutico , Hepatopatías/tratamiento farmacológico , Animales , Antioxidantes/síntesis química , Sitios de Unión , Dominio Catalítico , Cisteína/síntesis química , Cisteína/uso terapéutico , Citocromo P-450 CYP2E1/metabolismo , Citocromo P-450 CYP3A/metabolismo , Hepatocitos/efectos de los fármacos , Hepatocitos/metabolismo , Iminas/síntesis química , Hepatopatías/etiología , Hepatopatías/metabolismo , Masculino , Simulación del Acoplamiento Molecular , Estrés Oxidativo/efectos de los fármacos , RatasRESUMEN
The farnesoid X receptor (FXR) is a ligand-activated transcription factor that plays important roles in regulating bile acid homeostasis. The aim of the present study was to investigate the effects of obeticholic acid (OCA), a novel synthetic FXR agonist, carbon tetrachloride (CCl4)-induced acute liver injury. Mice were intraperitoneally injected with CCl4 (0.15ml/kg). In CCl4+OCA group, mice were orally with OCA (5mg/kg) 48, 24 and 1h before CCl4. As expected, hepatic FXR was activated by OCA. Interestingly, OCA pretreatment alleviated CCl4-induced elevation of serum ALT and hepatic necrosis. Moreover, OCA pretreatment inhibited CCl4-induced hepatocyte apoptosis. Additional experiment showed that OCA inhibits CCl4-induced hepatic chemokine gene Mcp-1, Mip-2 and Kc. Moreover, OCA inhibits CCl4-induced hepatic pro-inflammatory gene Tnf-α and Il-1ß. By contrast, OCA pretreatment elevated hepatic anti-inflammatory gene Il-4. Further analysis showed that OCA pretreatment inhibited hepatic IκB phosphorylation and blocked nuclear translocation of NF-κB p65 and p50 subunits during CCl4-induced acute liver injury. In addition, OCA pretreatment inhibited hepatic Akt, ERK and p38 phosphorylation in CCl4-induced acute liver injury. These results suggest that OCA protects against CCl4-induced acute liver injury and inflammation. Synthetic FXR agonists may be effective antidotes for hepatic inflammation during acute liver injury.
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Intoxicación por Tetracloruro de Carbono/prevención & control , Enfermedad Hepática Inducida por Sustancias y Drogas/prevención & control , Ácido Quenodesoxicólico/análogos & derivados , Inflamación/etiología , Alanina Transaminasa/sangre , Animales , Apoptosis/efectos de los fármacos , Intoxicación por Tetracloruro de Carbono/complicaciones , Enfermedad Hepática Inducida por Sustancias y Drogas/complicaciones , Ácido Quenodesoxicólico/farmacología , Quinasas MAP Reguladas por Señal Extracelular/metabolismo , Hepatocitos/química , Hepatocitos/efectos de los fármacos , Masculino , Ratones , Proteínas Proto-Oncogénicas c-akt/metabolismo , Receptores Citoplasmáticos y Nucleares/metabolismo , Proteínas Quinasas p38 Activadas por Mitógenos/metabolismoRESUMEN
Oxidative stress is one of the mechanisms involved in the acute carbon tetrachloride (CCl4)-induced hepatotoxicity. Since 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl, known as tempol, has powerful antioxidant properties, we investigated its potential hepatoprotective effects and the underlying mechanisms that may add further benefits for its clinical usefulness using an acute model of CCl4-induced hepatotoxicity. One hour after CCl4 induction of acute hepatotoxicity, mice were treated with a daily dose of 20 mg/kg/day tempol for 3 days. It was found that treatment of animals with tempol significantly negated the pathological changes in liver function parameters as well as histology induced by CCl4. In addition, tempol significantly ameliorated CCl4-induced lipid peroxidation and GSH depletion, and improved catalase activity. Furthermore, tempol alleviated the inflammation induced by CCl4 as indicated by reducing the liver expression level of nuclear factor-kappa B (NF-κB) and tumor necrosis factor-α (TNF-α). Finally, tempol significantly reduced expression level of the B-cell lymphoma-2 protein (Bcl-2) and active caspase-3 which are known markers of apoptosis. In conclusion, the present study provides important evidences for the promising hepatoprotective effects of tempol that can be explained by amelioration of oxidative stress mainly through replenishment of GSH, restoration of antioxidant enzyme activities, and reduction of lipid peroxides alongside its anti-inflammatory properties.
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Enfermedad Hepática Inducida por Sustancias y Drogas/tratamiento farmacológico , Óxidos N-Cíclicos/uso terapéutico , Animales , Biomarcadores/metabolismo , Intoxicación por Tetracloruro de Carbono/complicaciones , Enfermedad Hepática Inducida por Sustancias y Drogas/etiología , Enfermedad Hepática Inducida por Sustancias y Drogas/metabolismo , Masculino , Ratones , Marcadores de SpinRESUMEN
Carbon tetrachloride (CCl4) is widely used as an animal model of hepatotoxicity and the mechanisms have been arduously studied, however, the contribution of the sympathetic nervous system (SNS) in CCl4-induced acute hepatotoxicity remains controversial. It is also known that either CCl4 or SNS can affect systemic inflammatory responses. The aim of this study was to establish the effect of chemical sympathectomy with 6-hydroxydopamine (6-OHDA) in a mouse model of CCl4-induced acute hepatotoxicity and systemic inflammatory response. Mice exposed to CCl4 or vehicle were pretreated with 6-OHDA or saline. The serum levels of aminotransferases and alkaline phosphatase in the CCl4-poisoning mice with sympathetic denervation were significantly lower than those without sympathetic denervation. With sympathetic denervation, hepatocellular necrosis and fat infiltration induced by CCl4 were greatly decreased. Sympathetic denervation significantly attenuated CCl4-induced lipid peroxidation in liver and serum. Acute CCl4 intoxication showed increased expression of inflammatory cytokines/chemokines [eotaxin-2/CCL24, Fas ligand, interleukin (IL)-1α, IL-6, IL-12p40p70, monocyte chemoattractant protein-1 (MCP-1/CCL2), and tumor necrosis factor-α (TNF-α)], as well as decreased expression of granulocyte colony-stimulating factor and keratinocyte-derived chemokine. The overexpressed levels of IL-1α, IL-6, IL-12p40p70, MCP-1/CCL2, and TNF-α were attenuated by sympathetic denervation. Pretreatment with dexamethasone significantly reduced CCl4-induced hepatic injury. Collectively, this study demonstrates that the SNS plays an important role in CCl4-induced acute hepatotoxicity and systemic inflammation and the effect may be connected with chemical- or drug-induced hepatotoxicity and circulating immune response.
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Intoxicación por Tetracloruro de Carbono/fisiopatología , Hígado/patología , Sistema Nervioso Simpático/fisiopatología , Síndrome de Respuesta Inflamatoria Sistémica/fisiopatología , Fosfatasa Alcalina/sangre , Animales , Intoxicación por Tetracloruro de Carbono/sangre , Intoxicación por Tetracloruro de Carbono/complicaciones , Intoxicación por Tetracloruro de Carbono/tratamiento farmacológico , Quimiocina CCL2/sangre , Quimiocinas/sangre , Citocinas/sangre , Dexametasona/uso terapéutico , Masculino , Ratones , Ratones Endogámicos C57BL , Síndrome de Respuesta Inflamatoria Sistémica/sangre , Síndrome de Respuesta Inflamatoria Sistémica/tratamiento farmacológico , Síndrome de Respuesta Inflamatoria Sistémica/etiología , Transaminasas/sangreRESUMEN
UNLABELLED: Interleukin (IL)-20 is a proinflammatory cytokine of the IL-10 family and involved in rheumatoid arthritis, atherosclerosis, stroke, and osteoporosis. However, the pathophysiological roles of IL-20 in liver injury have not been extensively studied. We explored the involvement of IL-20 in liver injury and the therapeutic potential of IL-20 antagonists for treating liver fibrosis. Compared with normal liver tissue from healthy individuals, the amount of IL-20 was much higher in hepatocytes and hepatic stellate cells in liver biopsies from patients with fibrosis, cirrhosis, and hepatocellular carcinoma. Carbon tetrachloride (CCl4) treatment induced IL-20 that further up-regulated the expression of transforming growth factor (TGF)-ß1 and p21(WAF1) and resulted in cell cycle arrest in the Clone-9 rat hepatocyte cell line. IL-20 activated quiescent rat hepatic stellate cells (HSCs) and up-regulated TGF-ß1 expression. IL-20 also increased TGF-ß1, tumor necrosis factor (TNF)-α, and type I collagen (Col-I) expression, and promoted the proliferation and migration of activated HSCs. Serum IL-20 was significantly elevated in mice with short-term and long-term CCl4 -induced liver injury. In mice with short-term liver injury, anti-IL-20 monoclonal antibody (7E) and anti-IL-20 receptor (IL-20R1) monoclonal antibody (51D) attenuated hepatocyte damage caused by CCl4, TGF-ß1, and chemokine production. In mice with long-term liver injury, 7E and 51D inhibited CCl4 -induced cell damage, TGF-ß1 production, liver fibrosis, HSC activation, and extracellular matrix accumulation, which was caused by the reduced expression of tissue inhibitors of metalloproteinases as well as increased metalloproteinase expression and Col-I production. IL-20R1-deficient mice were protected from short-term and long-term liver injury. CONCLUSION: We identified a pivotal role of IL-20 in liver injury and showed that 7E and 51D may be therapeutic for liver fibrosis.
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Anticuerpos Monoclonales/uso terapéutico , Intoxicación por Tetracloruro de Carbono/metabolismo , Enfermedad Hepática Inducida por Sustancias y Drogas/metabolismo , Interleucinas/inmunología , Cirrosis Hepática/inmunología , Cirrosis Hepática/metabolismo , Receptores de Interleucina/inmunología , Animales , Intoxicación por Tetracloruro de Carbono/complicaciones , Estudios de Casos y Controles , Línea Celular , Enfermedad Hepática Inducida por Sustancias y Drogas/complicaciones , Modelos Animales de Enfermedad , Humanos , Interleucinas/antagonistas & inhibidores , Interleucinas/metabolismo , Cirrosis Hepática/etiología , Masculino , Ratones , Ratones Endogámicos C57BL , Ratas , Ratas Sprague-Dawley , Receptores de Interleucina/metabolismoRESUMEN
UNLABELLED: The zinc finger transcription factor GATA4 controls specification and differentiation of multiple cell types during embryonic development. In mouse embryonic liver, Gata4 is expressed in the endodermal hepatic bud and in the adjacent mesenchyme of the septum transversum. Previous studies have shown that Gata4 inactivation impairs liver formation. However, whether these defects are caused by loss of Gata4 in the hepatic endoderm or in the septum transversum mesenchyme remains to be determined. In this study, we have investigated the role of mesenchymal GATA4 activity in liver formation. We have conditionally inactivated Gata4 in the septum transversum mesenchyme and its derivatives by using Cre/loxP technology. We have generated a mouse transgenic Cre line, in which expression of Cre recombinase is controlled by a previously identified distal Gata4 enhancer. Conditional inactivation of Gata4 in hepatic mesenchymal cells led to embryonic lethality around mouse embryonic stage 13.5, likely as a consequence of fetal anemia. Gata4 knockout fetal livers exhibited reduced size, advanced fibrosis, accumulation of extracellular matrix components and hepatic stellate cell (HSC) activation. Haploinsufficiency of Gata4 accelerated CCl4 -induced liver fibrosis in adult mice. Moreover, Gata4 expression was dramatically reduced in advanced hepatic fibrosis and cirrhosis in humans. CONCLUSIONS: Our data demonstrate that mesenchymal GATA4 activity regulates HSC activation and inhibits the liver fibrogenic process.
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Regulación hacia Abajo , Factor de Transcripción GATA4/fisiología , Cirrosis Hepática/metabolismo , Hígado/embriología , Animales , Intoxicación por Tetracloruro de Carbono/complicaciones , Línea Celular , Matriz Extracelular/metabolismo , Células Estrelladas Hepáticas/fisiología , Humanos , Integrasas , Cirrosis Hepática/etiología , Mesodermo/metabolismo , Ratones , Ratones Transgénicos , FenotipoRESUMEN
Oxidative stress induced by free radicals is known to be a common cause of liver damage and hepatic fibrosis. Anise oil and its compounds have been identified to have antioxidant, anti-inflammatory and antifibrinogenic properties that may play a role in the management of hepatic disorders and promote liver regeneration. Thus, the purpose of the study was to evaluate the effects of anise oil on hepatotoxicity induced by carbon tetrachloride in adult male albino rats. Sixty male albino rats were divided into control group, CCL4-treated group that was injected with 1 mg /kg CCL4 intraperitoneally (ip), CCL4+anise oil-treated group that was injected with 1 mg /kg of CCL4 and 0.5 ml/ kg of anise oil (ip), and anise oil-treated group that was injected with 0.5 ml/kg of anise oil. Animals received treatment for 4 weeks and sacrificed 24 hours after the last administration. Livers were removed and processed for light and electron microscopy analysis. The CCL4-treated group revealed loss of normal architecture of hepatic lobules, steatosis, necrosis, cholestasis, portal congestion and progressed grading of lobular inflammation, ballooning degeneration and fibrosis. On the other hand, the CCL4 + anise group showed reduced liver damage and increased signs of regeneration. We conclude that anise oil has a protective effect on liver damage caused by CCL4and promotes liver regeneration (AU)
No disponible
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Animales , Masculino , Femenino , Ratas , Tetracloruro de Carbono/efectos adversos , Tetracloruro de Carbono/uso terapéutico , Intoxicación por Tetracloruro de Carbono/complicaciones , Intoxicación por Tetracloruro de Carbono/veterinaria , Estrés Oxidativo , Pimpinella/efectos adversos , Pimpinella/toxicidad , Microanálisis por Sonda Electrónica/métodos , Microanálisis por Sonda Electrónica/veterinaria , Fotomicrografía/métodosRESUMEN
The authors described three groups of patients after acute poisonings. In the first group were 60 patients after carbon tetrachioride poisoning, the second group consisted of 81 patients after mushroom poisoning and 20 patients after ethylene glycol poisoning were in the third group. Besides two patients with rare poisonings after potassium dichromate and after paraquat poisoning were analysed. All groups of patients with the kidney damage were presented from the diagnostic, differential diagnostic, conservative, ntra- and extracorporeal elimination treatment point of view. In the group of patients suffering from acute carbon tetrachloride poisoning and with acute renal failure following therapy was used: conservative treatment, exchange blood transfusion--in 4 patients in hepatic coma, renal replacement therapy (peritoneal dialysis, haemodialysis, plasmapheresis). From the total number of 60 patients 58 survived and 2 patients died in liver coma. Survival of patients after mushroom poisoning depended on amount of oral use of mushroom (Amanita phalloides), on early admission in dialysis centre and on early beginning of renal replacement therapy within 24 hr after acute poisoning. Twenty four patients from 81 patients of this group died. Main clinical signs of ethylene glycol poisoning were various neurological symptoms (cramps, hemiparesis, coma), severe metabolic acidosis (pH = 7.06 +/- 0.14), leucocytosis (26.4 +/- 5.5x 10(9)/L) and the signs of acute toxic hepatitis and of acute renal failure. Calcium oxalic crystals in urine were present in 17 patients and leucocytosis was observed in every patient. In the first 4 patients we administered intravenously ethylalcohol as an antidotum and later in other patients we used ethylalcohol in dialysis solution. The concentration of ethylalcohol in dialysis solution was 100 mg%. Severe metabolic acidosis improved in 17 patients using bicarbonate haemodialysis and 3 patients died before the possibility to use bicarbonate haemodialysis. Eighty-four hours after acute potassium dichromate poisoning and 24 hours after exchange blood transfusion during haemodialysis a 41-year old man died in haemorhagic shock, which developed after the extensive chemical burns of mucous membrane of gastrointestinal tract caused by this poison. Our patient after paraquat poisoning was treated by repeated charcoal haemoperfusion and haemodialysis. Despite of that therapy the patient died in severe respiratory insufficiency.
Asunto(s)
Lesión Renal Aguda/terapia , Intoxicación por Tetracloruro de Carbono/terapia , Sobredosis de Droga/terapia , Glicol de Etileno/envenenamiento , Intoxicación por Setas/terapia , Terapia de Reemplazo Renal , Lesión Renal Aguda/etiología , Adulto , Amanita , Transfusión Sanguínea , Quemaduras Químicas/etiología , Intoxicación por Tetracloruro de Carbono/complicaciones , Intoxicación por Tetracloruro de Carbono/mortalidad , Carbón Orgánico/uso terapéutico , Sobredosis de Droga/complicaciones , Sobredosis de Droga/mortalidad , Tracto Gastrointestinal/efectos de los fármacos , Tracto Gastrointestinal/lesiones , Humanos , Masculino , Intoxicación por Setas/complicaciones , Intoxicación por Setas/mortalidad , Paraquat/envenenamiento , Dicromato de Potasio/envenenamiento , Diálisis Renal , Choque Hemorrágico/etiología , Tasa de SupervivenciaRESUMEN
Liver fibrosis is a severe, life-threatening clinical condition resulting from nonresolving hepatitis of different origins. IL-17A is critical in inflammation, but its relation to liver fibrosis remains elusive. We find increased IL-17A expression in fibrotic livers from HBV-infected patients undergoing partial hepatectomy because of cirrhosis-related early-stage hepatocellular carcinoma in comparison with control nonfibrotic livers from uninfected patients with hepatic hemangioma. In fibrotic livers, IL-17A immunoreactivity localizes to the inflammatory infiltrate. In experimental carbon tetrachloride-induced liver fibrosis of IL-17RA-deficient mice, we observe reduced neutrophil influx, proinflammatory cytokines, hepatocellular necrosis, inflammation, and fibrosis as compared with control C57BL/6 mice. IL-17A is produced by neutrophils and T lymphocytes expressing the Th17 lineage-specific transcription factor Retinoic acid receptor-related orphan receptor γt. Furthermore, hepatic stellate cells (HSCs) isolated from naive C57BL/6 mice respond to IL-17A with increased IL-6, α-smooth muscle actin, collagen, and TGF-ß mRNA expression, suggesting an IL-17A-driven fibrotic process. Pharmacologic ERK1/2 or p38 inhibition significantly attenuated IL-17A-induced HSC activation and collagen expression. In conclusion, IL-17A(+) Retinoic acid receptor-related orphan receptor γt(+) neutrophils and T cells are recruited into the injured liver driving a chronic, fibrotic hepatitis. IL-17A-dependent HSC activation may be critical for liver fibrosis. Thus, blockade of IL-17A could potentially benefit patients with chronic hepatitis and liver fibrosis.
Asunto(s)
Células Estrelladas Hepáticas/metabolismo , Interleucina-17/fisiología , Cirrosis Hepática/etiología , Actinas/biosíntesis , Actinas/genética , Adulto , Animales , Intoxicación por Tetracloruro de Carbono/complicaciones , Carcinoma Hepatocelular/química , Carcinoma Hepatocelular/etiología , Carcinoma Hepatocelular/cirugía , Enfermedad Hepática Inducida por Sustancias y Drogas/etiología , Enfermedad Hepática Inducida por Sustancias y Drogas/metabolismo , Enfermedad Hepática Inducida por Sustancias y Drogas/patología , Colágeno/biosíntesis , Colágeno/genética , Citocinas/biosíntesis , Citocinas/genética , Femenino , Regulación de la Expresión Génica/efectos de los fármacos , Regulación de la Expresión Génica/inmunología , Hemangioma/química , Hemangioma/cirugía , Hepatectomía , Células Estrelladas Hepáticas/patología , Hepatitis B Crónica/complicaciones , Hepatitis Animal/inducido químicamente , Hepatitis Animal/metabolismo , Hepatitis Animal/patología , Humanos , Interleucina-17/análisis , Interleucina-17/biosíntesis , Interleucina-17/genética , Interleucina-17/farmacología , Cirrosis Hepática/complicaciones , Cirrosis Hepática/metabolismo , Cirrosis Hepática/patología , Neoplasias Hepáticas/química , Neoplasias Hepáticas/cirugía , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Sistema de Señalización de MAP Quinasas/fisiología , Masculino , Ratones , Ratones Noqueados , Persona de Mediana Edad , Neutrófilos/fisiología , Miembro 3 del Grupo F de la Subfamilia 1 de Receptores Nucleares/análisis , Inhibidores de Proteínas Quinasas/farmacología , Receptores de Interleucina-17/deficiencia , Proteínas Recombinantes/farmacología , Células Th17/inmunología , Células Th17/metabolismo , Factor de Crecimiento Transformador beta/biosíntesisRESUMEN
Since the 2011 nuclear accident in Fukushima, the effects of low-dose irradiation, especially internal exposure, are at the forefront of everyone's attention. However, low-dose radiation induced various stimulating effects such as activation of antioxidative and immune functions. In this study, we attempted to evaluate the quantitative effects of the activation of antioxidative activities in kidney induced by radon inhalation on carbon tetrachloride (CCl4)-induced renal damage. Mice were subjected to intraperitoneal (i.p.) injection of CCl4 after inhaling approximately 1000 or 2000 Bq/m3 radon for 24 h, or immediately after i.p. injection of α-tocopherol (100, 300, or 500 mg/kg bodyweight). In case of renal function, radon inhalation at a concentration of 2000 Bq/m3 has the inhibitory effects similar to α-tocopherol treatment at a dose of 300-500 mg/kg bodyweight. The activities of superoxide dismutase and catalase in kidneys were significantly higher in mice exposed to radon as compared to mice treated with CCl4 alone. These findings suggest that radon inhalation has an antioxidative effect against CCl4-induced renal damage similar to the antioxidative effects of α-tocopherol due to induction of antioxidative functions.
Asunto(s)
Lesión Renal Aguda/prevención & control , Intoxicación por Tetracloruro de Carbono/complicaciones , Riñón/patología , Radón/uso terapéutico , alfa-Tocoferol/uso terapéutico , Lesión Renal Aguda/etiología , Lesión Renal Aguda/patología , Animales , Antioxidantes/uso terapéutico , Tetracloruro de Carbono/toxicidad , Intoxicación por Tetracloruro de Carbono/tratamiento farmacológico , Intoxicación por Tetracloruro de Carbono/patología , Modelos Animales de Enfermedad , Femenino , Tasa de Filtración Glomerular/efectos de los fármacos , Riñón/efectos de los fármacos , Riñón/fisiopatología , Ratones , Ratones Endogámicos ICR , Resultado del TratamientoRESUMEN
AIM: To investigate the mechanisms underlying the protective effects of quercetin-rutinoside (rutin) and its aglycone quercetin against CCl(4)-induced liver damage in mice. METHODS: BALB/cN mice were intraperitoneally administered rutin (10, 50, and 150 mg/kg) or quercetin (50 mg/kg) once daily for 5 consecutive days, followed by the intraperitoneal injection of CCl(4) in olive oil (2 mL/kg, 10% v/v). The animals were sacrificed 24 h later. Blood was collected for measuring the activities of ALT and AST, and the liver was excised for assessing Cu/Zn superoxide dismutase (SOD) activity, GSH and protein concentrations and also for immunoblotting. Portions of the livers were used for histology and immunohistochemistry. RESULTS: Pretreatment with rutin and, to a lesser extent, with quercetin significantly reduced the activity of plasma transaminases and improved the histological signs of acute liver damage in CCl(4)-intoxicated mice. Quercetin prevented the decrease in Cu/Zn SOD activity in CCl(4)-intoxicated mice more potently than rutin. However, it was less effective in the suppression of nitrotyrosine formation. Quercetin and, to a lesser extent, rutin attenuated the inflammation in the liver by down-regulating the CCl(4)-induced activation of nuclear factor-kappa B (NF-κB), tumor necrosis factor-α (TNF-α) and cyclooxygenase (COX-2). The expression of inducible nitric oxide synthase (iNOS) was more potently suppressed by rutin than by quercetin. Treatment with both flavonoids significantly increased NF-E2-related factor 2 (Nrf2) and heme oxygenase (HO-1) expression in injured livers, although quercetin was less effective than rutin at an equivalent dose. Quercetin more potently suppressed the expression of transforming growth factor-ß1 (TGF-ß1) than rutin. CONCLUSION: Rutin exerts stronger protection against nitrosative stress and hepatocellular damage but has weaker antioxidant and anti-inflammatory activities and antifibrotic potential than quercetin, which may be attributed to the presence of a rutinoside moiety in position 3 of the C ring.
Asunto(s)
Intoxicación por Tetracloruro de Carbono/complicaciones , Enfermedad Hepática Inducida por Sustancias y Drogas/prevención & control , Depuradores de Radicales Libres/uso terapéutico , Hígado/efectos de los fármacos , Quercetina/uso terapéutico , Rutina/uso terapéutico , Animales , Compuestos de Bifenilo/química , Intoxicación por Tetracloruro de Carbono/enzimología , Intoxicación por Tetracloruro de Carbono/patología , Enfermedad Hepática Inducida por Sustancias y Drogas/enzimología , Enfermedad Hepática Inducida por Sustancias y Drogas/etiología , Enfermedad Hepática Inducida por Sustancias y Drogas/patología , Relación Dosis-Respuesta a Droga , Depuradores de Radicales Libres/administración & dosificación , Depuradores de Radicales Libres/química , Inmunohistoquímica , Inyecciones Intraperitoneales , Hígado/enzimología , Hígado/patología , Pruebas de Función Hepática , Masculino , Ratones , Ratones Endogámicos BALB C , Estructura Molecular , Óxido Nítrico/química , Estrés Oxidativo/efectos de los fármacos , Picratos/química , Quercetina/administración & dosificación , Quercetina/química , Rutina/administración & dosificación , Rutina/químicaRESUMEN
Increased levels of intestinal VEGF are thought to worsen portal hypertension. The cause of the increase in the level of intestinal VEGF found during cirrhosis is not known. The aim of this study is to demonstrate a relationship between portal pressure (PP) and intestinal/ plasma VEGF levels in different stages of fibrosis/cirrhosis. In this experiment, rats were exposed to carbon tetrachloride (CCl(4) ) for 6, 8 and 12 weeks. At the end of exposure, the three groups of rats exhibited three different stages of pathology: non-cirrhotic, early fibrotic and cirrhotic, respectively. For those rats and their age-matched controls, PP and intestinal/plasma VEGF levels were measured. Rats inhaling CCl(4) for 12 weeks developed portal hypertension (18.02 ± 1.07 mmHg), while those exposed for 6 weeks (7.26 ± 0.58 mmHg) and for 8 weeks (8.55 ± 0.53 mmHg) did not. The rats exposed for 12 weeks also showed a 40% increase in the level of intestinal VEGF compared to the controls (P < 0.05), while those rats exposed to CCl(4) inhalation for 6 and 8 weeks did not. There was a significant positive correlation between PP and intestinal VEGF levels (r(2) = 0.4, P < 0.005). Plasma VEGF levels were significantly elevated in those rats exposed to 12 weeks of CCl(4) inhalation (63.7 pg/ml, P < 0.01), compared to the controls (8.5 pg/ml). However, no correlation was observed between PP and plasma VEGF levels. It is concluded that portal pressure modulates intestinal VEGF levels during the development of cirrhosis.