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INTRODUCTION: Double centrifugation before freezing is recommended before thrombin generation assays (TGA). However, this procedure is not mandatory for routine hemostasis tests, precluding the use of these samples for TGA. The aim of this study is to assess the impact of single and double centrifugation on TGA performed on frozen samples from healthy volunteers (HVs) and patients receiving direct oral anticoagulants (DOACs). METHODS: Forty HVs and 57 patients receiving a DOAC (dabigatran, rivaroxaban, apixaban, or edoxaban) were included in this prospective double-center observational study. Blood was collected into 109 mmol/L citrated tubes and frozen at -70°C before TGA using ST Genesia with STG-DrugScreen reagent. Four pre-analytical conditions were studied: (A) single centrifugation (2000 g, 15 minutes) before freezing; (B) one centrifugation before freezing and another after thawing (2000 g, 15 minutes for both); (C) one centrifugation before freezing(2000 g, 15 minutes) and another after thawing (2000 g, 10 minutes); (D) double centrifugation (2000 g, 15 minutes) before freezing (reference). Centrifugation conditions (A), (B), and (C) were compared with the reference condition (D). Acceptable relative differences were defined at 6%, 8%, and 10% for normalized lag time, endogenous thrombin potential, and peak height, respectively. RESULTS: Centrifugation conditions had a small but acceptable impact on HVs samples, but single centrifugation always resulted in unacceptable reductions in normalized lag times for DOAC samples. A second centrifugation after thawing permitted the recovery of acceptable differences for the three TGA parameters for edoxaban but not for apixaban, rivaroxaban, nor dabigatran. CONCLUSION: Double centrifugation before freezing should remain the recommended pre-analytical condition before TGA.
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Anticoagulantes/administración & dosificación , Pruebas de Coagulación Sanguínea/normas , Coagulación Sanguínea/efectos de los fármacos , Centrifugación , Trombina/biosíntesis , Administración Oral , Pruebas de Coagulación Sanguínea/métodos , Centrifugación/efectos adversos , Voluntarios Sanos , HumanosRESUMEN
BACKGROUND: Technological advancements in modern military and acrobatic jet planes have resulted in extraordinary psychophysiological loads being exerted upon flying personnel, including inducing neck and back pain. The purpose of this study was to examine the effects of 12 weeks of functional strength training on 1) the volume and strength of the neck and shoulder muscles and 2) muscular activity upon exposure to helmets of different masses and elevated Gz forces in a long-arm centrifuge in high-performance aircraft personnel. METHODS: Eighteen participants underwent 12 weeks of functional strength training (n = 12) or the control protocol (n = 6) without additional strength training. Pre- and post-intervention tests included evaluations of isometric strength of the head extensor muscles, flexion, and lateral flexion and rotation, as well as magnetic resonance imaging (MRI) to measure the volume of the m. sternocleidomastoideus, m. trapezius, and deep neck muscles. Furthermore, during a long-arm centrifuge (+ 1.4 and + 3 Gz) protocol, the muscular activity levels of the m. sternocleidomastoideus, m. trapezius and m. erector spinae muscles were assessed without a flight helmet, with a helmet, and with a helmet and night vision goggles. Each participant's perception of muscular strain was noted immediately after the long-arm centrifuge protocol. RESULTS: The maximal isometric strength in all exercises and muscle volumes increased in the training group but not the control group (P < 0.05). Relative muscle activity (%MVC) with a helmet decreased after the intervention in the training but not the control group (P = 0.01). Relative muscle activity while wearing a helmet and night vision goggles was higher after intervention in the control group than in the training group (P < 0.01). The perceived muscular strain of the neck muscles induced by the long-arm centrifuge did not differ between the groups. CONCLUSION: Twelve weeks of functional strength training improves the maximal isometric strength and volume of neck and shoulder muscles and leads to lower relative muscle activation upon exposure to elevated Gz forces in a long-arm centrifuge.
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Centrifugación/efectos adversos , Gravitación , Fuerza Muscular/fisiología , Entrenamiento de Fuerza/métodos , Entrenamiento de Fuerza/normas , Medicina Aeroespacial/métodos , Aeronaves/instrumentación , Centrifugación/métodos , Humanos , Entrenamiento de Fuerza/estadística & datos numéricosRESUMEN
BACKGROUND: Lipofilling is one of the most often performed surgical procedures in plastic and reconstructive surgery. Lipoaspirates provide a ready source of stem cells and secreted factors that contribute to neoangiogenesis and fat graft survival. However, the regulations about the enrichment of these beneficial cells and factors are ambiguous. In this study, the authors tested whether a combination of centrifugation and homogenization allowed the enrichment of viable stem cells in lipoaspirates through the selective removal of tumescent solution, blood, and released lipids without significantly affecting the cell secretome. METHODS: Human lipoaspirate was harvested from six different patients using water jet-assisted liposuction. Lipoaspirate was homogenized by first centrifugation (3584 rpm for 2 minutes), shear strain (10 times intersyringe processing), and second centrifugation (3584 rpm for 2 minutes). Stem cell enrichment was shown by cell counting after stem cell isolation. Lipoaspirate from different processing steps (unprocessed, after first centrifugation, after homogenization, after second centrifugation) was incubated in serum-free cell culture medium for mass spectrometric analysis of secreted proteins. RESULTS: Lipoaspirate homogenization leads to a significant 2.6 ± 1.75-fold enrichment attributable to volume reduction without reducing the viability of the stem cells. Protein composition of the secretome did not change significantly after tissue homogenization. Considering the enrichment effects, there were no significant differences in the protein concentration of the 83 proteins found in all processing steps. CONCLUSIONS: Stem cells can be enriched mechanically without significantly affecting the composition of secreted proteins. Shear-assisted enrichment of lipoaspirate constitutes no substantial manipulation of the cells' secretome.
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Tejido Adiposo/citología , Proteoma/metabolismo , Trasplante de Células Madre , Células Madre/metabolismo , Adulto , Anciano , Contorneado Corporal/métodos , Recuento de Células , Separación Celular/métodos , Centrifugación/efectos adversos , Medio de Cultivo Libre de Suero , Femenino , Supervivencia de Injerto/fisiología , Voluntarios Sanos , Humanos , Lipectomía/métodos , Masculino , Espectrometría de Masas , Persona de Mediana Edad , Neovascularización Fisiológica/fisiología , Cultivo Primario de Células/métodos , Proteoma/análisis , Proteómica , Resistencia al CorteRESUMEN
Blood Concentrates (BCs) are autologous non-transfusional therapeutical preparations with biological properties applied in tissue regeneration. These BCs differ in the preparation method, in fibrin network architecture, growth factors release as well as in platelet/cell content. Methodological changes result in distinct matrices that can compromise their clinical effectiveness. The present study evaluated the influence of different g-forces and types of tubes in the release of vascular endothelial growth factor (VEGF) from platelet-rich fibrin (PRF) as a function of time. The PRF-like samples were obtained with three g-forces (200, 400, and 800 x g) for 10 minutes in pure glass tubes or in polystyrene-clot activator tubes. Scanning and Transmission electron microscopy was used to morphometric analyzes of PRF's specimens and flow cytometry was used to quantify VEGF slow release until 7 days. Our results showed that platelets were intact and adhered to the fibrin network, emitting pseudopods and in degranulation. The fibrin network was rough and twisted with exosomic granulations impregnated on its surface. An increase in the concentration of VEGF in the PRF supernatant was observed until 7 days for all g forces (200, 400 or 800 xg), with the highest concentrations observed with 200 x g, in both tubes, glass or plastic. Morphological analyzes showed a reduction in the diameter of the PRF fibers after 7 days. Our results showed that g-force interferes with the shape of the fibrin network in the PRF, as well as affect the release of VEGF stored into platelets. This finding may be useful in applying PRF to skin lesions, in which the rapid release of growth factors can favor the tissue repair process. Our observations point to a greater clarification on the methodological variations related to obtaining PRF matrices, as they can generate products with different characteristics and degrees of effectiveness in specific applications.
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Plaquetas/metabolismo , Fibrinólisis , Fibrina Rica en Plaquetas/metabolismo , Ingeniería de Tejidos/métodos , Factor A de Crecimiento Endotelial Vascular/metabolismo , Plaquetas/ultraestructura , Centrifugación/efectos adversos , Centrifugación/métodos , Femenino , Fibrina/metabolismo , Fibrina/ultraestructura , Voluntarios Sanos , Humanos , Microscopía Electrónica de Rastreo , Microscopía Electrónica de Transmisión , Factor A de Crecimiento Endotelial Vascular/análisisRESUMEN
Artificial gravity elicited through short-arm human centrifugation combined with physical exercise, such as jumping, is promising in maintaining health and performance during space travel. However, motion sickness symptoms could limit the tolerability of the approach. Therefore, we determined the feasibility and tolerability, particularly occurrence of motion sickness symptoms, during reactive jumping exercises on a short-arm centrifuge. In 15 healthy men, we assessed motion sickness induced by jumping exercises during short-arm centrifugation at constant +1Gz or randomized variable +0.5, +0.75, +1, +1.25 and +1.5 Gz along the body axis referenced to center of mass. Jumping in the upright position served as control intervention. Test sessions were conducted on separate days in a randomized and cross-over fashion. All participants tolerated jumping exercises against terrestrial gravity and on the short-arm centrifuge during 1 Gz or variable Gz at the center of mass without disabling motion sickness symptoms. While head movements markedly differed, motion sickness scores were only modestly increased with jumping on the short-arm centrifuge compared with vertical jumps. Our study demonstrates that repetitive jumping exercises are feasible and tolerable during short-arm centrifugation. Since jumping exercises maintain muscle and bone mass, our study enables further development of exercise countermeasures in artificial gravity.
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Centrifugación/efectos adversos , Gravedad Alterada/efectos adversos , Mareo por Movimiento/etiología , Vuelo Espacial , Adaptación Fisiológica , Adulto , Medicina Aeroespacial , Centrifugación/instrumentación , Ejercicio Físico/fisiología , Gravitación , Movimientos de la Cabeza/fisiología , Voluntarios Sanos , Humanos , Masculino , Mareo por Movimiento/fisiopatología , Mareo por Movimiento/prevención & control , Medidas contra la Ingravidez , Adulto JovenRESUMEN
BACKGROUND AND OBJECTIVES: Although HLA-eliminated platelets can facilitate transfusions to patients possessing HLA antibodies, no such products are currently available commercially perhaps because the platelet collection rate is not yet economically viable. We have improved this process' efficiency by employing a hollow-fibre system at the last step of the production process after an acid and a reaction buffer have been washed out conventionally by centrifugation. MATERIALS AND METHODS: HLA-eliminated platelets were prepared via four distinct steps: chilled on ice, treated with an acid solution, diluted and finally washed using the hollow-fibre system. The efficiency of this platelet recovery process was determined. The resulting products' platelet characteristics, including a capacity for HLA expression, were evaluated in vitro and compared in detail to their corresponding originals. RESULTS: The average efficiency of platelet recovery was 91%. Although the expression levels of CD62P, a molecular marker for platelet activation, were approximately threefold higher on new platelets than on the original platelets, their HLA expression levels were lower. The phagocytosis assay, with monoclonal antibodies and cognate HLA antibody-containing sera, suggested that HLA-ABC molecules on the cell surface were sufficiently removed. The platelet functions, including the agonist-induced aggregability and adherence/aggregability of the collagen-coated plates under certain conditions, were conserved and not significantly different from the original ones. CONCLUSION: We propose a novel preparation system for producing HLA-eliminated platelets without centrifugation, which ensures a highly efficient, and therefore, much more economical method of platelet recovery that also retains their key functionality.
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Plaquetas/citología , Separación Celular/métodos , Anticuerpos Monoclonales/inmunología , Plaquetas/inmunología , Separación Celular/instrumentación , Separación Celular/normas , Centrifugación/efectos adversos , Antígenos HLA/inmunología , Humanos , Selectina-P/genética , Selectina-P/metabolismo , Activación PlaquetariaRESUMEN
BACKGROUND: UK Royal Air Force fast jet aircrew use three different anti-G systems, however, little objective comparison of the G protection they provide exists. The G-protection afforded by each system and associated hemodynamic responses were investigated.METHODS: Ten subjects performed centrifuge acceleration exposures using Mk-10 (S1) and Mk-4 (S2) five-bladder anti-G trousers (AGT) and full coverage AGT plus pressure breathing for G-protection (PBG; S3). Measurements of relaxed G tolerance (RGT), eye-level blood pressure (BPeye), lower body blood volume (LBV), stroke volume (SV) and total peripheral resistance (TPR) were made during gradual onset runs (GOR) and rapid onset runs (ROR). The subjective effort required to maintain clear vision at +7 and +8 Gz provided an indication of the protection provided by the system.RESULTS: All systems moderated decreases in SV and BPeye and increases in LBV under increased +Gz. S3 provided the greatest mean RGT during GOR (+6.2 Gz) and ROR (+6 Gz), reduced the effort required to maintain clear vision at up to +8 Gz, prevented venous pooling and afforded the greatest rise in TPR. The majority of indices revealed no difference between S1 and S2 although RGT during the ROR was greater with S2 (+0.25 Gz).DISCUSSION: S3 effectively prevented pooling of blood in the lower limbs under +Gz, despite the use of PBG, and offers an advantage over five-bladder AGT. Given the similarities of S1 and S2, it was unsurprising that the majority of indices measured were similar. The objective measurement of hemodynamic parameters provides useful information for comparing the G-protection provided by anti-G systems.Pollock RD, Firth RV, Storey JA, Phillips KE, Connolly DM, Green NDC, Stevenson AT. Hemodynamic responses and G protection afforded by three different anti-G systems. Aerosp Med Hum Perform. 2019; 90(11):925-933.
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Medicina Aeroespacial/instrumentación , Trajes Gravitatorios , Hemodinámica/fisiología , Hipergravedad/efectos adversos , Personal Militar , Aceleración/efectos adversos , Adulto , Centrifugación/efectos adversos , Humanos , Masculino , Reino Unido , Adulto JovenRESUMEN
BACKGROUNDArtificial gravity (AG) has the potential to provide a comprehensive countermeasure mitigating deleterious effects of microgravity. However, the cross-coupled "Coriolis" illusion has prevented using a more feasible and less costly short-radius centrifuge, as compared to large, slowly spinning systems.OBJECTIVEWe assessed tolerability of a personalized, incremental protocol to acclimate humans to the cross-coupled illusion, enabling faster spin rates.METHODSTen subjects were exposed to the illusion by performing roll head tilts while seated upright and spun about an Earth-vertical axis. The spin rate was incremented when head tilts did not subjectively elicit the illusion. Subjects completed one 25-minute session on each of 10 days.RESULTSThe spin rate at which subjects felt no cross-coupled illusion increased in all subjects from an average of 1.8 rotations per minute (RPM) (SD: ±0.9) at the beginning of the protocol to 17.7 RPM (SD: ±9.1) at the end. For off-axis centrifugation producing 1G at the rider's feet, this corresponds to a reduction in the required centrifuge diameter from 552.2 to 5.7 meters. Subjects reported no more than slight motion sickness.CONCLUSIONSAcclimation to the cross-coupled illusion, such as that accomplished here, is critical for feasibility of short-radius centrifugation for AG implementation.
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Adaptación Fisiológica/fisiología , Gravedad Alterada , Ilusiones/fisiología , Mareo por Movimiento/prevención & control , Adolescente , Adulto , Centrifugación/efectos adversos , Centrifugación/métodos , Tolerancia al Ejercicio/fisiología , Femenino , Gravedad Alterada/efectos adversos , Movimientos de la Cabeza/fisiología , Inclinación de Cabeza/fisiología , Humanos , Ilusiones/psicología , Individualidad , Masculino , Mareo por Movimiento/fisiopatología , Mareo por Movimiento/psicología , Factores de Tiempo , Adulto JovenRESUMEN
Mechanical circulatory support devices have been used clinically for patients with heart failure for over 10 years. However, thrombus formation inside blood pumps remains a risk to patient life, causing pump failure and contributing to neurological damage through embolization. In this article, we propose a method for preventing thrombus formation by applying vibrational excitation to the impeller. We evaluate the ability of this method to enhance the antithrombogenic properties of a magnetically levitated centrifugal blood pump and ensure that the impeller vibration does not cause undue hemolysis. First, 3 vibrational conditions were compared using an isolated pump without a mock circulation loop; the vibrational excitation frequencies and amplitudes for the impeller were set to (a) 0 Hz-0 µm, (b) 70 Hz-10 µm, and (c) 300 Hz-2.5 µm. The motor torque was measured to detect thrombus formation and obtain blood coagulation time by calculating the derivative of the torque. Upon thrombus detection, the pump was stopped and thrombi size were evaluated. The results showed an increase in the blood coagulation time and a decrease in the rate of thrombus formation in pumps with the impeller vibration. Second, an in vitro hemolysis test was performed for each vibrational condition to determine the effect of impeller vibration on hemolysis. The results revealed that there was no significant difference in hemolysis levels between each condition. Finally, the selected vibration based on the above test results and the non-vibration as control were compared to investigate antithrombogenic properties under the continuous flow condition. The blood coagulation time and thrombi size were investigated. As a result, vibrational excitation of the impeller at a frequency of 300 Hz and amplitude of 2.5 µm was found to significantly lengthen clotting time, decreasing the rate of pump thrombus compared to the non-vibration condition. We indicate the potential of impeller vibration as a novel mechanical antithrombogenic mechanism for rotary blood pumps.
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Circulación Asistida/instrumentación , Corazón Auxiliar/efectos adversos , Trombosis/etiología , Animales , Circulación Asistida/efectos adversos , Coagulación Sanguínea , Centrifugación/efectos adversos , Centrifugación/instrumentación , Diseño de Equipo , Hemólisis , Humanos , Magnetismo/instrumentación , Porcinos , Trombosis/sangre , Trombosis/prevención & control , VibraciónRESUMEN
Mechanical forces are essential drivers of numerous biological processes, notably during development. Although it is well recognized that cells sense and adapt to mechanical forces, the signal transduction pathways that underlie mechanosensing have remained elusive. Here, we investigate the impact of mechanical centrifugation force on phosphorylation-mediated signaling in Xenopus embryos. By monitoring temporal phosphoproteome and proteome alterations in response to force, we discover and validate elevated phosphorylation on focal adhesion and tight junction components, leading to several mechanistic insights into mechanosensing and tissue restoration. First, we determine changes in kinase activity profiles during mechanoresponse, identifying the activation of basophilic kinases. Pathway interrogation using kinase inhibitor treatment uncovers a crosstalk between the focal adhesion kinase (FAK) and protein kinase C (PKC) in mechanoresponse. Second, we find LIM domain 7 protein (Lmo7) as upregulated upon centrifugation, contributing to mechanoresponse. Third, we discover that mechanical compression force induces a mesenchymal-to-epithelial transition (MET)-like phenotype.
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Fenómenos Mecánicos , Proteoma/metabolismo , Transducción de Señal , Xenopus laevis/metabolismo , Animales , Centrifugación/efectos adversos , Embrión no Mamífero/metabolismo , Proteína-Tirosina Quinasas de Adhesión Focal/metabolismo , Fosforilación , Proteína Quinasa C/metabolismo , Procesamiento Proteico-PostraduccionalRESUMEN
BACKGROUND: Exposure to high sustained +Gz (head-to-foot inertial load) is known to have harmful effects on pilots' body in flight. Although clinical data have shown that liver dysfunction occurs in pilots, the precise cause has not been well defined. AIM: To investigate rat liver function changes in response to repeated +Gz exposure. METHODS: Ninety male Wistar rats were randomly divided into a blank control group (BC group, n = 30), a +6 Gz/5 min stress group (6GS group, n = 30), and a +10 Gz/5min stress group (10GS group, n = 30). The 6GS and 10GS groups were exposed to +6 Gz and +10 Gz, respectively, in an animal centrifuge. The onset rate of +Gz was 0.5 G/s. The sustained time at peak +Gz was 5 min for each exposure (for 5 exposures, and 5-min intervals between exposures for a total exposure and non-exposure time of 50 min). We assessed liver injury by measuring the portal venous flow volume, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), liver tissue malondialdehyde (MDA), Na+-K+-ATPase, and changes in liver histology. These parameters were recorded at 0 h, 6 h, and 24 h after repeated +Gz exposures. RESULTS: After repeated +Gz exposures in the 6GS and the 10GS groups, the velocity and flow signal in the portal vein (PV) were significantly decreased as compared to the BC group at 0 h after exposure. Meanwhile, we found that the PV diameter did not change significantly. However, rats in the 6GS group had a much higher portal venous flow volume than the 10GS group at 0 h after exposure. The 6GS group had significantly lower ALT, AST, and MDA values than the 10GS group 0 h and 6 h post exposure. The Na+-K+-ATPase activity in the 6GS group was significantly higher than that in the 10GS group 0 h and 6 h post exposure. Hepatocyte injury, determined pathologically, was significantly lower in the 6GS group than in the 10GS group. CONCLUSION: Repeated +Gz exposures transiently cause hepatocyte injury and affect liver metabolism and morphological structure.
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Aceleración/efectos adversos , Hipergravedad/efectos adversos , Hepatopatías/fisiopatología , Hígado/lesiones , Estrés Fisiológico , Medicina Aeroespacial , Animales , Velocidad del Flujo Sanguíneo , Centrifugación/efectos adversos , Modelos Animales de Enfermedad , Hepatocitos/metabolismo , Hepatocitos/patología , Humanos , Hígado/irrigación sanguínea , Hígado/citología , Hígado/patología , Hepatopatías/etiología , Pruebas de Función Hepática , Masculino , Pilotos , Vena Porta/fisiopatología , Ratas , Ratas WistarRESUMEN
The cell membrane of ram spermatozoa is more sensitive to the freezing process than in other species due to its composition. As a result, the quality and viability of frozen thawed ram spermatozoa are often poor, which together with the specific structure of the ewe's cervix are the main reasons for lower fertility in ewes after intracervical insemination. In the present study we investigated the effects of semen centrifugation through a single layer of a species-specific colloid (Androcoll-O) on post-thaw quality of ram spermatozoa. Motility, viability and morphology were analysed 0, 6, 12 and 24â¯h after thawing. DNA fragmentation index (%DFI) of the samples was assessed 0â¯h after thawing, by SCSA™. Membrane and acrosome integrity of spermatozoa were analysed by Sybr-14/PI/PNA test 0â¯h after thawing. The proportion of motile spermatozoa was significantly higher in SLC - selected samples in comparison to control (not SLC - selected) samples at 0, 6, 12 (Pâ¯<â¯0.001) and 24â¯hâ¯(Pâ¯<â¯0.05). The proportion of viable spermatozoa was also significantly higher in SLC - selected samples in comparison to control samples at all times (Pâ¯<â¯0.001). The proportion of abnormal acrosomes and morphologically abnormal spermatozoa (MAS) were significantly lower in SLC - selected samples compared to control samples at all times (Pâ¯<â¯0.001). Analysis of chromatin stability revealed significantly lower %DFI values in SLC - selected samples compared to control samples (Pâ¯<â¯0.001). The SYBR-14/PI/PNA test also revealed significantly better values in SLC - selected compared to control samples (Pâ¯<â¯0.05). In conclusion, single layer colloid centrifugation significantly improved post-thaw quality and longevity of ram spermatozoa, making it suitable for artificial insemination initiatives.
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Centrifugación/métodos , Criopreservación/métodos , Análisis de Semen , Preservación de Semen/métodos , Acrosoma/efectos de los fármacos , Animales , Membrana Celular/efectos de los fármacos , Membrana Celular/fisiología , Centrifugación/efectos adversos , Cromatina , Coloides , Femenino , Fertilidad , Congelación , Inseminación Artificial , Masculino , Semen/efectos de los fármacos , Semen/fisiología , Ovinos , Motilidad Espermática/efectos de los fármacos , Motilidad Espermática/fisiologíaRESUMEN
BACKGROUNDS: The BD Vacutainer® Barricor™ Plasma collection tube (BD Barricor) uses an innovative non-gel separation method. This study compared the plasma residual cell count (PRCC) obtained from BD Barricor and from BD PST II plasma tubes. METHODS: Four BD Barricors and one BD PST II were collected from 40 donors. BD PST II was centrifuged at 1300g/10â¯min, while the BD Barricors were centrifuged at 1800g/10â¯min, 4000g/3â¯min, 4000g/7â¯min and 4000g/15â¯min. PRCC was evaluated measuring white blood cells (WBC), red blood cells (RBC) and Platelets (PLT) counts by Siemens ADVIA 2120. Cell-free hemoglobin was quantified by haemolysis index (HI) by Roche Cobas c501. RESULTS: BD PST II Median WBC, RBC and PLT counts were 0.38 (109/L), 0.0291 (1012/L) and 113.5 (109/L), respectively. Considering the BD PST II as reference, PRCC differences were expressed as median bias percentage. WBC showed a significant reduction at all the conditions (pâ¯<â¯0.01), being the reductions: 63.9% (1800g/10â¯min), 69.9% (4000g/3â¯min), 75.0% (4000g/7â¯min) and 82.7% (4000g/15â¯min). RBC reductions 29.7% (1800g/10â¯min), 33.8% (4000g/3â¯min), 39.6% (4000g/7â¯min) and 66.4 (4000g/15â¯min) were all significant (pâ¯<â¯0.01). PLT reductions were 1.6% at 1800g/10â¯min (pâ¯=â¯ns), 1.2% at 4000g/3â¯min (pâ¯=â¯ns), 27.1% at 4000g/7â¯min (pâ¯=â¯0.046) and 46.6% at 4000g/15â¯min (pâ¯=â¯0.005). BD Barricor centrifuged for 7 and 15â¯min at 4000g showed an increased haemolysis. CONCLUSIONS: BD Barricors plasma quality improved with increasing the centrifugation times but already at 4000g/3â¯min, the suggested centrifugation condition, a significant improvement was achieved.
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Recuento de Células Sanguíneas/normas , Recolección de Muestras de Sangre/métodos , Recolección de Muestras de Sangre/instrumentación , Centrifugación/efectos adversos , Hemólisis , HumanosRESUMEN
The Japan Aerospace Exploration Agency recently performed a mouse experiment in the International Space Station in which mice were raised for 35 days, retrieved using the Dragon spacecraft, and then harvested for analysis 2 days after splashdown. However, the impact of the retrieval procedure, which exposed mice to 5-10 g for 2 min during atmospheric reentry and splashdown, was unknown. Therefore, the purpose of this study was to examine the impact of a 10 g load for 2 min (using a gondola-type centrifuge with a 1.5-m arm installed at Gifu University) on conscious mice. Plasma corticosterone increased at 30 min after load application and recovered at 90 min. Significant Fos expression was observed in the vestibular nuclei (VeN), paraventricular hypothalamic nucleus (PVN), and central nucleus of the amygdala (CeA). Rearing behavior and food intake were suppressed. Mice with vestibular lesions demonstrated increased corticosterone and Fos expression in the PVN, but neither suppression of food intake and rearing behavior nor increased Fos expression in the VeN and CeA. These results suggest that the simulated gravity load induced a transient stress response, hypoactivity, and a vestibular-mediated suppression of food intake.
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Centrifugación/efectos adversos , Estado de Conciencia , Gravedad Alterada/efectos adversos , Vuelo Espacial , Estrés Fisiológico , Animales , Biomarcadores/sangre , Núcleo Amigdalino Central/metabolismo , Núcleo Amigdalino Central/fisiopatología , Centrifugación/métodos , Corticosterona/sangre , Ingestión de Alimentos , Conducta Alimentaria , Ratones Endogámicos C57BL , Mareo por Movimiento/etiología , Mareo por Movimiento/metabolismo , Mareo por Movimiento/fisiopatología , Actividad Motora , Núcleo Hipotalámico Paraventricular/metabolismo , Núcleo Hipotalámico Paraventricular/fisiopatología , Proteínas Proto-Oncogénicas c-fos/metabolismo , Factores de Tiempo , Núcleos Vestibulares/metabolismo , Núcleos Vestibulares/fisiopatologíaRESUMEN
BACKGROUND: Centrifugation (Cf) is a common method of fat processing but may be time consuming, especially when processing large volumes. OBJECTIVES: To determine the effects on fat grafting time, volume efficiency, reoperations, and complication rates of Cf vs an autologous fat processing system (Rv) that incorporates fat harvesting and processing in a single unit. METHODS: We performed a retrospective cohort study of consecutive patients who underwent autologous fat grafting during reconstructive breast surgery with Rv or Cf. Endpoints measured were volume of fat harvested (lipoaspirate) and volume injected after processing, time to complete processing, reoperations, and complications. A budget impact model was used to estimate cost of Rv vs Cf. RESULTS: Ninety-eight patients underwent fat grafting with Rv, and 96 patients received Cf. Mean volumes of lipoaspirate (506.0 vs 126.1 mL) and fat injected (177.3 vs 79.2 mL) were significantly higher (P < .0001) in the Rv vs Cf group, respectively. Mean time to complete fat grafting was significantly shorter in the Rv vs Cf group (34.6 vs 90.1 minutes, respectively; P < .0001). Proportions of patients with nodule and cyst formation and/or who received reoperations were significantly less in the Rv vs Cf group. Based on these outcomes and an assumed per minute operating room cost, an average per patient cost savings of $2,870.08 was estimated with Rv vs Cf. CONCLUSIONS: Compared to Cf, the Rv fat processing system allowed for a larger volume of fat to be processed for injection and decreased operative time in these patients, potentially translating to cost savings. LEVEL OF EVIDENCE 3.
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Tejido Adiposo/trasplante , Centrifugación/economía , Técnicas Cosméticas/economía , Eficiencia Organizacional , Costos de la Atención en Salud , Lipectomía/economía , Quirófanos/economía , Quirófanos/organización & administración , Procedimientos de Cirugía Plástica/economía , Procedimientos de Cirugía Plástica/métodos , Recolección de Tejidos y Órganos/economía , Recolección de Tejidos y Órganos/métodos , Adulto , Anciano , Presupuestos , Centrifugación/efectos adversos , Técnicas Cosméticas/efectos adversos , Ahorro de Costo , Análisis Costo-Beneficio , Femenino , Humanos , Lipectomía/efectos adversos , Persona de Mediana Edad , Modelos Económicos , Complicaciones Posoperatorias/economía , Complicaciones Posoperatorias/etiología , Procedimientos de Cirugía Plástica/efectos adversos , Estudios Retrospectivos , Factores de Tiempo , Recolección de Tejidos y Órganos/efectos adversos , Trasplante Autólogo , Resultado del Tratamiento , Flujo de Trabajo , Adulto JovenRESUMEN
As of today, there exist no reliable, objective methods for early detection of thrombi in the extracorporeal membrane oxygenators (ECMO) system. Within the ECMO system, thrombi are not always fixed to a certain component or location in the circuit. Thus, clot fragments of different shapes and consistencies may circulate and give rise to vibrations and sound generation. By bedside sound measurements and additional laboratory experiments (although not detailed herein), we found that the presence of particles (clots or aggregates and fragments of clots) can be detected by analyzing the strength of infra-sound (< 20 Hz) modes of the spectrum near the inlet and outlet of the centrifugal pump in the ECMO circuit. For the few patients that were considered in this study, no clear false positive or negative examples were found when comparing the spectral approach with clinical observations. A laboratory setup provided insight to the flow in and out of the pump, confirming that in the presence of particles a low-amplitude low-frequency signal is strongly amplified, enabling the identification of a clot.
Asunto(s)
Oxigenación por Membrana Extracorpórea/efectos adversos , Trombosis/diagnóstico , Trombosis/etiología , Acústica , Centrifugación/efectos adversos , Centrifugación/instrumentación , Diseño de Equipo , Oxigenación por Membrana Extracorpórea/instrumentación , Femenino , Humanos , Masculino , Oxigenadores de Membrana/efectos adversos , SonidoRESUMEN
BACKGROUND: Centrifuge training, while an integral component in pilot training, is not without risks. To date there has never been a reported case of isolated transverse process fractures associated with centrifuge training. CASE REPORT: A 32-yr-old Flight Surgeon underwent centrifuge training as part of an educational course. She had increasing back pain after exposure to the centrifuge. Follow-up studies showed left L2 and bilateral L3 transverse process fractures. No other contributory causes could be identified except for mild vitamin D deficiency. DISCUSSION: The etiology, incidence, and treatment of transverse process fractures are examined to better prepare the clinician for the management of these cases. Puderbaugh MA. A report of transverse process fractures secondary to the centrifuge in a healthy aviator. Aerosp Med Hum Perform. 2016; 87(7):655-658.
Asunto(s)
Accidentes de Trabajo , Centrifugación/efectos adversos , Gravitación , Vértebras Lumbares/lesiones , Pilotos , Fracturas de la Columna Vertebral/etiología , Femenino , HumanosRESUMEN
Though blood bank processing traditionally employs centrifugation, new separation techniques may be appealing for large scale processes. Split-flow fractionation (SPLITT) is a family of techniques that separates in absence of labelling and uses very low flow rates and force fields, and is therefore expected to minimize cell damage. However, the hydrodynamic stress and possible consequent damaging effects of SPLITT fractionation have not been yet examined. The aim of this study was to investigate the hydrodynamic damage of SPLITT fractionation to human red blood cells, and to compare these effects with those induced by centrifugation. Peripheral whole blood samples were collected from healthy volunteers. Samples were diluted in a buffered saline solution, and were exposed to SPLITT fractionation (flow rates 1-10 ml/min) or centrifugation (100-1500 g) for 10 min. Cell viability, shape, diameter, mean corpuscular hemoglobin, and membrane potential were measured. Under the operating conditions employed, both SPLITT and centrifugation maintained cell viability above 98%, but resulted in significant sublethal damage, including echinocyte formation, decreased cell diameter, decreased mean corpuscular hemoglobin, and membrane hyperpolarization which was inhibited by EGTA. Wall shear stress and maximum energy dissipation rate showed significant correlation with lethal and sublethal damage. Our data do not support the assumption that SPLITT fractionation induces very low shear stress and is innocuous to cell function. Some changes in SPLITT channel design are suggested to minimize cell damage. Measurement of membrane potential and cell diameter could provide a new, reliable and convenient basis for evaluation of hydrodynamic effects on different cell models, allowing identification of optimal operating conditions on different scales.
Asunto(s)
Separación Celular/métodos , Centrifugación/efectos adversos , Centrifugación/métodos , Eritrocitos/citología , Eritrocitos/fisiología , Fenómenos Biomecánicos/fisiología , Separación Celular/instrumentación , Forma de la Célula/fisiología , Supervivencia Celular/fisiología , Centrifugación/instrumentación , Diseño de Equipo , Membrana Eritrocítica/fisiología , Humanos , Hidrodinámica , Potenciales de la Membrana/fisiología , Resistencia al Corte/fisiologíaRESUMEN
BACKGROUND: Varicocele is quite common in the general population, affecting up to 15% of men. It is not considered disqualifying for the pilot's training program of the Israeli Air Force as long as there are no related symptoms or associated pathologies. During combat flight, increased venous pressure due to acceleration forces and anti-G straining maneuvers, used to counteract high gravitational G forces, can theoretically aggravate the venous blood pooling in varicocele, leading to rupture. CASE REPORT: We describe a case of a young fighter-jet pilot presenting with a painful inguinal hematoma extending to the scrotum a day after participating in centrifuge training. Sonographic examination demonstrated dilated spermatic veins and intratesticular varicocele along with subcutaneous thickening of the scrotal wall consistent with hematoma. DISCUSSION: The effects of high G loads on blood flow in spermatic veins, and especially in varicocele, still need to be determined. Varicocele rupture has been described in relation to increased intra-abdominal pressure and could theoretically occur during anti-G straining maneuvers. Such an acute adverse event during combat flight can be detrimental to flight safety and the pilot's well-being.
Asunto(s)
Medicina Aeroespacial , Enfermedades Asintomáticas , Centrifugación/efectos adversos , Hematoma/etiología , Personal Militar , Entrenamiento Simulado , Varicocele/complicaciones , Hematoma/diagnóstico por imagen , Humanos , Israel , Masculino , Escroto/diagnóstico por imagen , Ultrasonografía , Varicocele/diagnóstico por imagen , Adulto JovenRESUMEN
INTRODUCTION: Many countries have hypergravity training centers using centrifuges for pilots to cope with a high gravity (G) environment. The high G training carries potential risk for the development of spinal injury. However, no studies evaluated the influence of centrifuge training on the spines of asymptomatic fighter pilots on a large scale. METHODS: Study subjects were 991 male fighter pilots with high G training at one institution. Subject variables included information about physical characteristics, flight hours of pilots prior to the training, and G force exposure related factors during training. The two dependent variables were whether the pilots developed acute spinal injury after training and the severity of the injury (major/minor). RESULTS: The incidence of acute spinal injury after high G training was 2.3% (23 of 991 subjects). There were 19 subjects who developed minor injury and 4 subjects who developed a herniated intervertebral disc, which is considered a major injury. In multivariate analysis, only the magnitude of G force during training was significantly related to the development of acute spinal injury. However, there was no significant factor related to the severity of the injury. DISCUSSION: These results suggest that high G training could cause negative effects on fighter pilots' spines. The magnitude of G force during training seemed to be the most significant factor affecting the occurrence of acute spinal injury.