RESUMEN
Chagas disease (CD) is caused by the parasite Trypanosoma cruzi. CD affects people worldwide, primarily in tropical areas. The central nervous system (CNS) is an essential site for T. cruzi persistence during infection. The protozoan may pass through the blood-brain barrier and may cause motor and cognitive neuronal damage. Once in the CNS, T. cruzi triggers immune responses that the purinergic system can regulate. Treatment for CD is based on benznidazole (BNZ); however, this agent has negative side-effects and is toxic to the host. For this reason, we investigated whether resveratrol (RSV), a potent antioxidant and neuroprotective molecule, would modulate purinergic signaling and RSV alone or in combination with BNZ would prevent changes in purinergic signaling and oxidative damage caused by T. cruzi. We infected mice with T. cruzi and treated them with RSV or BNZ for 8 days. Increases in ATP and ADP hydrolysis by NTPDase in the total cortex of infected animals were observed. The treatment with RSV in infected group diminished ATP, ADP, and AMP hydrolysis compared to infected group. The combination of RSV + BNZ decreased AMP hydrolysis in infected animals compared to the INF group, exerting an anti-inflammatory effect. RSV acted as a neuroprotector, decreasing adenosine levels. Infected animals presented an increase of P2X7 and A2A density of purine receptors. RSV reduced P2X7 and A2A and increased A1 density receptors in infected animals. In addition, infected animals showed higher TBARS and reactive oxygen species (ROS) levels than control. RSV diminished ROS levels in infected mice, possibly due to antioxidant properties. In short, we conclude that resveratrol could act as a neuroprotective molecule, probably preventing inflammatory changes caused by infection by T. cruzi, even though the mice experienced high levels of parasitemia.
Asunto(s)
Corteza Cerebral/efectos de los fármacos , Corteza Cerebral/metabolismo , Enfermedad de Chagas/metabolismo , Nitroimidazoles/administración & dosificación , Receptores Purinérgicos/biosíntesis , Resveratrol/administración & dosificación , Enfermedad Aguda , Animales , Antioxidantes/administración & dosificación , Corteza Cerebral/parasitología , Enfermedad de Chagas/tratamiento farmacológico , Femenino , Expresión Génica , Inmunosupresores/administración & dosificación , Ratones , Estrés Oxidativo/efectos de los fármacos , Estrés Oxidativo/fisiología , Receptores Purinérgicos/genéticaRESUMEN
The central nervous system of the intermediate host plays a central role in lifelong persistence of Toxoplasma gondii as well as the pathogenesis of congenital toxoplasmosis and reactivated infection in immunocompromised individuals. The purinergic system has been implicated in a wide range of immunological pathways for controlling intracellular responses to pathogens, including T. gondii. In the present study, we investigated the effect of resveratrol (RSV) on ectonucleotidases, adenosine deaminase (ADA), and purinergic receptors during chronic infection by T. gondii. For this study, Swiss mice were divided into control (CTL), resveratrol (RSV), infected (INF), and INF+RSV groups. The animals were orally infected with the VEG strain and treated with RSV (100 mg/kg, orally). Ectonucleotidase activities, P2X7, P2Y1, A1, and A2A purinergic receptor density, ROS, and thiobarbituric acid reactive substances levels were measured in the cerebral cortex of mice. T. gondii infection increased NTPDase and reduced ADA activities. Treatment with RSV also affected enzymes hydrolysing extracellular nucleotides and nucleosides. Finally, RSV affected P1 and P2 purinergic receptor expression during T. gondii infection. Overall, RSV-mediated beneficial changes in purinergic signalling and oxidative stress, possibly improving cerebral cortex homeostasis in T. gondii infection.
Asunto(s)
Corteza Cerebral/parasitología , Inhibidores Enzimáticos/farmacología , Fármacos Neuroprotectores/farmacología , Resveratrol/farmacología , Toxoplasmosis Animal/tratamiento farmacológico , Adenosina Desaminasa/metabolismo , Animales , Ratones , Receptores Purinérgicos/metabolismo , Transducción de Señal , Toxoplasma/inmunologíaRESUMEN
Astrocytes and microglia are the most abundant glial cells. They are responsible for physiological support and homeostasis maintenance in the central nervous system (CNS). The increasing evidences of their involvement in the control of infectious diseases justify the emerging interest in the improvement of methodologies to isolate primary astrocytes and microglia in order to evaluate their responses to infections that affect the CNS. Considering the impact of Trypanosoma cruzi (T. cruzi) and Toxoplasma gondii (T. gondii) infection in the CNS, here we provide a method to extract, maintain, dissociate and infect murine astrocytes and microglia cells with protozoa parasites. Extracted cells from newborn cortices are maintained in vitro for 14 days with periodic differential media replacement. Astrocytes and microglia are obtained from the same extraction protocol by mechanical dissociation. After phenotyping by flow cytometry, cells are infected with protozoa parasites. The infection rate is determined by fluorescence microscopy at different time points, thus enabling the evaluation of differential ability of glial cells to control protozoan invasion and replication. These techniques represent simple, cheap and efficient methods to study the responses of astrocytes and microglia to infections, opening the field for further neuroimmunology analysis.
Asunto(s)
Astrocitos/citología , Microglía/citología , Enfermedades Parasitarias/patología , Animales , Animales Recién Nacidos , Astrocitos/parasitología , Técnicas de Cultivo de Célula , Corteza Cerebral/citología , Corteza Cerebral/parasitología , Ratones , Microglía/parasitología , Enfermedades Parasitarias/parasitología , Toxoplasma/fisiología , Trypanosoma cruzi/fisiologíaRESUMEN
BACKGROUND: The protozoan parasite Toxoplasma gondii infects and alters the neurotransmission in cerebral cortex and other brain regions, leading to neurobehavioral and neuropathologic changes in humans and animals. However, the molecules that contribute to these changes remain largely unknown. METHODS: We have investigated the impact of T. gondii infection on the overall metabolism of mouse cerebral cortex. Mass-spectrometry-based metabolomics and multivariate statistical analysis were employed to discover metabolomic signatures that discriminate between cerebral cortex of T. gondii-infected and uninfected control mice. RESULTS: Our results identified 73, 67 and 276 differentially abundant metabolites, which were involved in 25, 37 and 64 pathways at 7, 14 and 21 days post-infection (dpi), respectively. Metabolites in the unsaturated fatty acid biosynthesis pathway were upregulated as the infection progressed, indicating that T. gondii induces the biosynthesis of unsaturated fatty acids to promote its own growth and survival. Some of the downregulated metabolites were related to pathways, such as steroid hormone biosynthesis and arachidonic acid metabolism. Nine metabolites were identified as T. gondii responsive metabolites, namely galactosylsphingosine, arachidonic acid, LysoSM(d18:1), L-palmitoylcarnitine, calcitetrol, 27-Deoxy-5b-cyprinol, L-homophenylalanine, oleic acid and ceramide (d18:1/16:0). CONCLUSIONS: Our data provide novel insight into the dysregulation of the metabolism of the mouse cerebral cortex during T. gondii infection and have important implications for studies of T. gondii pathogenesis.
Asunto(s)
Corteza Cerebral/metabolismo , Corteza Cerebral/parasitología , Interacciones Huésped-Parásitos , Toxoplasmosis Animal/patología , Toxoplasmosis Cerebral/patología , Animales , Encéfalo/patología , Regulación hacia Abajo , Femenino , Espectrometría de Masas , Redes y Vías Metabólicas , Metabolómica , Ratones , Ratones Endogámicos BALB C , Análisis Multivariante , Toxoplasma , Regulación hacia ArribaAsunto(s)
Encefalopatías/diagnóstico por imagen , Corteza Cerebral/diagnóstico por imagen , Imagen por Resonancia Magnética , Enfermedad de Whipple/diagnóstico por imagen , Anciano , Enfermedades de los Ganglios Basales/diagnóstico por imagen , Enfermedades de los Ganglios Basales/patología , Biopsia , Encefalopatías/patología , Corteza Cerebral/parasitología , Ventrículos Cerebrales/diagnóstico por imagen , Ventrículos Cerebrales/patología , Medios de Contraste , Diagnóstico Diferencial , Humanos , Aumento de la Imagen , Masculino , Putamen/diagnóstico por imagen , Putamen/patología , Enfermedad de Whipple/patologíaRESUMEN
Chagas disease is caused by the protozoan parasite Trypanosoma cruzi and causes severe cardiac and brain damage, leading to behavioral alterations in humans and animals. However, the mechanisms involved in memory impairment during T. cruzi infection remain unknown. It has long been recognized that the enzymatic activities of acetylcholinesterase (AChE) and Na+, K+-ATPase are linked with memory dysfunction during other trypanosomiasis. Thus, the aim of this study was to evaluate the involvement of cerebral AChE and Na+, K+-ATPase activities in the memory impairment during T. cruzi (Colombian strain) infection. A significant decrease on latency time during the inhibitory avoidance task was observed in animals infected by T. cruzi compared to uninfected animals, findings compatible to memory dysfunction. Moreover, the cerebral AChE activity increased, while the Na+, K+-ATPase decreased in T. cruzi infected compared to uninfected animals. Histopathology revealed mild to moderate multifocal gliosis in the cerebral cortex and light focal meningeal lymphoplasmacytic infiltrate, which may have contributed to memory loss. Based on these evidences, we can conclude that T. cruzi (Colombian strain) causes memory impairment in mice experimentally infected. Moreover, the changes in AChE and Na+, K+-ATPase activities may be considered a mechanism involved in disease pathogenesis.
Asunto(s)
Acetilcolinesterasa/metabolismo , Infecciones Protozoarias del Sistema Nervioso Central/enzimología , Corteza Cerebral/enzimología , Trastornos de la Memoria/enzimología , ATPasa Intercambiadora de Sodio-Potasio/metabolismo , Trypanosoma cruzi/patogenicidad , Animales , Conducta Animal , Encéfalo/enzimología , Encéfalo/parasitología , Encéfalo/patología , Infecciones Protozoarias del Sistema Nervioso Central/parasitología , Infecciones Protozoarias del Sistema Nervioso Central/patología , Infecciones Protozoarias del Sistema Nervioso Central/psicología , Corteza Cerebral/parasitología , Corteza Cerebral/patología , Enfermedad de Chagas , Modelos Animales de Enfermedad , Femenino , Gliosis/enzimología , Gliosis/parasitología , Gliosis/patología , Corazón , Humanos , Trastornos de la Memoria/parasitología , Trastornos de la Memoria/patología , Trastornos de la Memoria/psicología , Ratones , Tripanosomiasis/parasitología , Tripanosomiasis/psicologíaRESUMEN
Neurological symptoms have been associated with Leishmania infection, however little is known about how the nervous system is affected in leishmaniasis. This work aimed to analyze parasitic load, production of cytokines/neurotrophins in the prefrontal cortex and behavioral changes in BALB/c mice infected with Leishmania amazonensis. At 2 and 4months post-infection, infected mice showed a decrease in IFN-γ, IL-1, IL-6, IL-4, IL-10 cytokines and BDNF and NGF neurotrophins in prefrontal cortex associated with increased anxiety behavior. Parasite DNA was found in brain of all animals at 4months post-infection, when the levels of IBA-1 (activated macrophage/microglia marker) and TNF-α was increased in the prefrontal cortex. However TNF-α returned to normal levels at 6months post-infection suggesting a neuroprotective mechanism.
Asunto(s)
Corteza Cerebral/metabolismo , Citocinas/metabolismo , Leishmaniasis/complicaciones , Leishmaniasis/patología , Trastornos Mentales/etiología , Factores de Crecimiento Nervioso/metabolismo , Animales , Proteínas de Unión al Calcio/genética , Proteínas de Unión al Calcio/metabolismo , Corteza Cerebral/parasitología , ADN Protozoario/genética , ADN Protozoario/metabolismo , Modelos Animales de Enfermedad , Conducta Exploratoria , Regulación de la Expresión Génica , Leishmania mexicana/genética , Leishmania mexicana/patogenicidad , Leishmaniasis/microbiología , Masculino , Aprendizaje por Laberinto/fisiología , Trastornos Mentales/parasitología , Ratones , Ratones Endogámicos BALB C , Proteínas de Microfilamentos/genética , Proteínas de Microfilamentos/metabolismo , Piel/patología , Factores de TiempoRESUMEN
BACKGROUND AND PURPOSE: The purpose of this study was to analyze the computed tomography (CT) and magnetic resonance imaging (MRI) features of cerebral sparganosis to improve the accuracy of diagnosing cerebral sparganosis with medical imaging modalities. MATERIALS AND METHODS: This was a retrospective study of CT and MRI features of 12 patients with cerebral sparganosis. A comparative analysis between imaging findings, and intraoperative and postoperative pathological findings was performed. RESULTS: A total of 20 lesions were observed in 12 patients with 5 patients having a solitary lesion. CT and MRI imaging showed worm-body sign in 5 patients (41.7%), tunnel-sign in 5 patients (41.7%), migration sign in 7 patients (58.3%), worm-shaped enhancement in 4 patients (33.3%), bead-shaped or ring-shaped enhancement in 5 patients (41.7%), irregular or nodular enhancement in 3 patients (25%), meningeal enhancement in 2 patients (16.6%), intracranial hemorrhage in 2 patients (16.6%), brain parenchymal edema in 10 patients (83.3%), cerebral white matter degeneration in 11 patients (91.7%), negative mass effect in 10 patients (83.3%), and punctuate calcification in 3 patients (25%). Among the 4 patients with live worm, CT and MRI showed worm-body sign in 3 patients (75%), tunnel-sign in 3 patients (75%), migration sign in 3 patients (75%), and worm-shaped enhancement in 2 patients (50%). CONCLUSION: Cerebral sparganosis with live worm exhibits several distinguishing imaging characteristics, which reflect the pathological changes and can improve the diagnosis of cerebral sparganosis.
Asunto(s)
Corteza Cerebral/diagnóstico por imagen , Corteza Cerebral/patología , Imagen por Resonancia Magnética , Esparganosis/diagnóstico por imagen , Tomografía Computarizada por Rayos X/métodos , Adolescente , Adulto , Anciano , Corteza Cerebral/parasitología , Niño , Femenino , Humanos , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Adulto JovenRESUMEN
Chronic infection with Toxoplasma gondii becomes established in tissues of the central nervous system, where parasites may directly or indirectly modulate neuronal function. Epidemiological studies have revealed that chronic infection in humans is a risk factor for developing mental diseases. However, the mechanisms underlying parasite-induced neuronal dysfunction in the brain remain unclear. Here, we examined memory associated with conditioned fear in mice and found that T. gondii infection impairs consolidation of conditioned fear memory. To examine the brain pathology induced by T. gondii infection, we analyzed the parasite load and histopathological changes. T. gondii infects all brain areas, yet the cortex exhibits more severe tissue damage than other regions. We measured neurotransmitter levels in the cortex and amygdala because these regions are involved in fear memory expression. The levels of dopamine metabolites but not those of dopamine were increased in the cortex of infected mice compared with those in the cortex of uninfected mice. In contrast, serotonin levels were decreased in the amygdala and norepinephrine levels were decreased in the cortex and amygdala of infected mice. The levels of cortical dopamine metabolites were associated with the time spent freezing in the fear-conditioning test. These results suggest that T. gondii infection affects fear memory through dysfunction of the cortex and amygdala. Our findings provide insight into the mechanisms underlying the neurological changes seen during T. gondii infection.
Asunto(s)
Amígdala del Cerebelo/fisiopatología , Corteza Cerebral/fisiopatología , Miedo/fisiología , Consolidación de la Memoria/fisiología , Memoria a Corto Plazo/fisiología , Enfermedades del Sistema Nervioso/parasitología , Toxoplasma/fisiología , Toxoplasmosis Animal , Amígdala del Cerebelo/parasitología , Análisis de Varianza , Animales , Conducta Animal/fisiología , Biomarcadores/análisis , Corteza Cerebral/parasitología , Cromatografía Líquida de Alta Presión , Masculino , Ratones , Ratones Endogámicos C57BL , Enfermedades del Sistema Nervioso/fisiopatología , Carga de Parásitos , Toxoplasmosis Animal/parasitología , Toxoplasmosis Animal/fisiopatologíaRESUMEN
BACKGROUND AND PURPOSE: The early diagnosis of cerebral paragonimiasis (CP) is essential for a good prognosis. We seek to provide references for early diagnosis by analyzing the imaging characteristics of cerebral paragonimiasis. MATERIALS AND METHODS: Images of 27 patients with CP (22 males and 5 females; median age 20.3 years; range: 4 to 47 years) were retrospectively evaluated. All patients underwent head computed tomography (CT) scans; 22 patients underwent conventional magnetic resonance imaging (MRI) sequences, including contrast-enhanced MRI for 20 patients and diffusion-weighted-imaging (DWI) for 1 patient. The diagnosis was confirmed based on a positive antibody test using enzyme-linked immunosorbent assay (ELISA) for paragonimiasis in the serum. RESULTS: The most common imaging findings of CP were isodense or hypodense lesions combined with extensive hypodense areas of perilesional edema on CT scans and a large mass composed of multiple ring-shaped lesions with surrounding edema on MRI images. The conglomeration of multiple ring-shaped lesions (n=11 patients), "tunnel signs" (n=12 patients) and worm-eaten signs (n=5 patients) were characteristic of most CP images. In 14 patients, contrast-enhanced MRI showed varying degrees of contrast enhancement combined with adjacent meningeal enhancement (n=10). CONCLUSIONS: A large mass comprising multiple ring-shaped lesions of different sizes, "tunnel signs" and worm-eaten signs with surrounding edema are the most characteristic features of CP. Extensive invasions of the adjacent meninges and ventricular wall (19 patients), multiple intracerebral lesions, bilateral hemispheric involvement, and lesion migration are other noteworthy imaging characteristics.
Asunto(s)
Corteza Cerebral/diagnóstico por imagen , Corteza Cerebral/patología , Imagen por Resonancia Magnética , Paragonimiasis/diagnóstico por imagen , Paragonimiasis/patología , Tomografía Computarizada por Rayos X , Adolescente , Adulto , Corteza Cerebral/parasitología , Niño , Preescolar , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto JovenRESUMEN
Exposure to the neurotropic parasite, Toxoplasma gondii, causes significant brain and behavioral anomalies in humans and other mammals. Understanding the cellular mechanisms of T. gondii-generated brain pathologies would aid the advancement of novel strategies to reduce disease. Complement factor C1q is part of a classic immune pathway that functions peripherally to tag and remove infectious agents and cellular debris from circulation. In the developing and adult brain, C1q modifies neuronal architecture through synapse marking and pruning. T. gondii exposure and complement activation have both been implicated in the development of complex brain disorders such as schizophrenia. Thus, it seems logical that mechanistically, the physiological pathways associated with these two factors are connected. We employed a rodent model of chronic infection to investigate the extent to which cyst presence in the brain triggers activation of cerebral C1q. Compared to uninfected mice, cortical C1q was highly expressed at both the RNA and protein levels in infected animals bearing a high cyst burden. In these mice, C1q protein localized to cytoplasm, adjacent to GFAP-labeled astrocytes, near degenerating cysts, and in punctate patterns along processes. In summary, our results demonstrated an upregulation of cerebral C1q in response to latent T. gondii infection. Our data preliminarily suggest that this complement activity may aid in the clearance of this parasite from the CNS and in so doing, have consequences for the connectivity of neighboring cells and synapses.
Asunto(s)
Corteza Cerebral/inmunología , Corteza Cerebral/parasitología , Complemento C1q/metabolismo , Toxoplasmosis/inmunología , Animales , Enfermedad Crónica , Quistes/inmunología , Femenino , RatonesRESUMEN
Cerebral malaria claims the lives of over 600,000 African children every year. To better understand the pathogenesis of this devastating disease, we compared the cellular dynamics in the cortical microvasculature between two infection models, Plasmodium berghei ANKA (PbA) infected CBA/CaJ mice, which develop experimental cerebral malaria (ECM), and P. yoelii 17XL (PyXL) infected mice, which succumb to malarial hyperparasitemia without neurological impairment. Using a combination of intravital imaging and flow cytometry, we show that significantly more CD8(+) T cells, neutrophils, and macrophages are recruited to postcapillary venules during ECM compared to hyperparasitemia. ECM correlated with ICAM-1 upregulation on macrophages, while vascular endothelia upregulated ICAM-1 during ECM and hyperparasitemia. The arrest of large numbers of leukocytes in postcapillary and larger venules caused microrheological alterations that significantly restricted the venous blood flow. Treatment with FTY720, which inhibits vascular leakage, neurological signs, and death from ECM, prevented the recruitment of a subpopulation of CD45(hi) CD8(+) T cells, ICAM-1(+) macrophages, and neutrophils to postcapillary venules. FTY720 had no effect on the ECM-associated expression of the pattern recognition receptor CD14 in postcapillary venules suggesting that endothelial activation is insufficient to cause vascular pathology. Expression of the endothelial tight junction proteins claudin-5, occludin, and ZO-1 in the cerebral cortex and cerebellum of PbA-infected mice with ECM was unaltered compared to FTY720-treated PbA-infected mice or PyXL-infected mice with hyperparasitemia. Thus, blood brain barrier opening does not involve endothelial injury and is likely reversible, consistent with the rapid recovery of many patients with CM. We conclude that the ECM-associated recruitment of large numbers of activated leukocytes, in particular CD8(+) T cells and ICAM(+) macrophages, causes a severe restriction in the venous blood efflux from the brain, which exacerbates the vasogenic edema and increases the intracranial pressure. Thus, death from ECM could potentially occur as a consequence of intracranial hypertension.
Asunto(s)
Barrera Hematoencefálica/inmunología , Corteza Cerebral/inmunología , Malaria Cerebral/inmunología , Plasmodium berghei/inmunología , Plasmodium yoelii/inmunología , Animales , Barrera Hematoencefálica/patología , Linfocitos T CD8-positivos/inmunología , Linfocitos T CD8-positivos/patología , Corteza Cerebral/parasitología , Corteza Cerebral/patología , Claudina-5/inmunología , Modelos Animales de Enfermedad , Clorhidrato de Fingolimod , Humanos , Inmunosupresores/farmacología , Molécula 1 de Adhesión Intercelular/inmunología , Macrófagos/inmunología , Macrófagos/patología , Malaria Cerebral/tratamiento farmacológico , Malaria Cerebral/patología , Ratones , Neutrófilos/inmunología , Neutrófilos/patología , Ocludina/inmunología , Glicoles de Propileno/farmacología , Esfingosina/análogos & derivados , Esfingosina/farmacología , Proteína de la Zonula Occludens-1/inmunologíaAsunto(s)
Antihelmínticos/uso terapéutico , Corteza Cerebral/parasitología , Praziquantel/uso terapéutico , Esparganosis/tratamiento farmacológico , Esparganosis/patología , Adulto , Corteza Cerebral/efectos de los fármacos , Corteza Cerebral/patología , Estudios de Seguimiento , Humanos , Imagen por Resonancia Magnética , Masculino , Tomografía de Emisión de PositronesRESUMEN
Cardiac ecchinococcosis is a rare disease. Its incidence varies from 0.02-2%. Commonly seen in the left ventricle arising from the myocardium in the subepicardial region.We report a 15-year-old boy presented with a rare combination of a left ventricular subendocardial hydatid cyst associated with multiple cysts in the left cerebral hemisphere and right posterior occipital lobe. The patient underwent successful surgical excision of the left ventricular hydatid cyst using cardiopulmonary bypass.
Asunto(s)
Encefalopatías/parasitología , Corteza Cerebral/parasitología , Equinococosis/diagnóstico , Cardiopatías/parasitología , Ventrículos Cardíacos/parasitología , Adolescente , Albendazol/uso terapéutico , Anticestodos/uso terapéutico , Encefalopatías/tratamiento farmacológico , Encefalopatías/patología , Procedimientos Quirúrgicos Cardíacos , Puente Cardiopulmonar , Corteza Cerebral/patología , Equinococosis/patología , Equinococosis/cirugía , Cardiopatías/diagnóstico , Cardiopatías/patología , Cardiopatías/cirugía , Ventrículos Cardíacos/patología , Ventrículos Cardíacos/cirugía , Humanos , MasculinoRESUMEN
Schistosomiasis is a parasitic disease caused by trematode worms from the Schistosoma genus and is characterized by high rates of morbidity. The main organs affected in this pathology, such as liver, kidneys and spleen, are shifted to a pro-oxidant state in the course of the infection. Here, we compared oxidative stress parameters of liver, kidney and spleen with other organs affected by schistosomiasis - heart, brain cortex and lungs. The results demonstrated that mice infected with Schistosoma mansoni had altered non-enzymatic antioxidant status in lungs and brain, increased carbonyl levels in lungs, and a moderate level of oxidative stress in heart. A severe redox imbalance in liver and kidneys and decreased non-enzymatic antioxidant capacity in spleen were also observed. Superoxide dismutase and catalase activities were differently modulated in liver, kidney and heart, and we found that differences in Superoxide dismutase 2 and catalase protein content may be responsible for these differences. Lungs had decreased receptor for advanced glycation endproduct expression and the brain cortex presented altered tau expression and phosphorylation levels, suggesting important molecular changes in these tissues, as homeostasis of these proteins is widely associated with the normal function of their respective organs. We believe that these results demonstrate for the first time that changes in the redox profile and expression of tissue-specific proteins of organs such as heart, lungs and brain are observed in early stages of S. mansoni infection.
Asunto(s)
Estrés Oxidativo/inmunología , Receptores Inmunológicos/metabolismo , Schistosoma mansoni/fisiología , Esquistosomiasis mansoni/inmunología , Proteínas tau/metabolismo , Animales , Catalasa/metabolismo , Corteza Cerebral/parasitología , Corteza Cerebral/patología , Regulación de la Expresión Génica/fisiología , Pulmón/parasitología , Pulmón/patología , Ratones , Miocardio/patología , Oxidación-Reducción , Receptor para Productos Finales de Glicación Avanzada , Receptores Inmunológicos/genética , Esquistosomiasis mansoni/metabolismo , Esquistosomiasis mansoni/parasitología , Superóxido Dismutasa , Proteínas tau/genéticaRESUMEN
INTRODUCTION: The cause of epilepsy in multiple sclerosis (MS) has not yet been elucidated. The relevance of cortical pathology (cortical lesions and thickness) in MS patients with and without epilepsy was evaluated in a longitudinal study. METHODS: 32 relapsing-remitting MS patients with epilepsy (RRMS/E) and 60 matched RRMS patients without epilepsy were included in a 3 year longitudinal study. The following clinical and MR parameters were analysed: Expanded Disability Status Scale (EDSS), cognitive score (CS), cortical lesion (CL) number and volume, grey matter fraction (GMf), global cortical thickness (CTh), T2 white matter lesion volume (T2WMLV), new CLs and new WM lesions. RESULTS: At baseline (T0), CLs were observed in 27/32 (84.4%) RRMS/E and in 26/60 (43.3%) RRMS (p<0.001) patients, and the RRMS/E group had a higher number (10.2 ± 8.9 vs 4.5 ± 2.4; p<0.001) and total volume (2.0 ± 1.3 vs 0.7 ± 0.8 cm(3); p<0.001) of CLs compared with the RRMS group. No significant difference in T2WMLV was observed. Global CTh was lower in RRMS/E (2.12 ± 0.19 vs 2.35 ± 0.14 mm; p<0.001), and this group also showed a decline in cognition (CS 10.9 ± 6.3 vs 6.2 ± 3.5; p<0.001). After 3 years (T1), the RRMS/E group had a higher accumulation of new CLs (3.4 ± 3.2 vs 1.2 ± 1.1; p<0.001) and faster reduction of GMf (p=0.022) while the two groups did not differ in the number of new WM and new Gad+ lesions. DISCUSSION: RRMS/E had a more severe and rapidly evolving cortical pathology (CLs and atrophy) compared with RRMS without epilepsy. The RRMS/E group was also characterised by more pronounced cognitive decline, higher EDSS and higher prevalence of men.
Asunto(s)
Corteza Cerebral/parasitología , Epilepsia/complicaciones , Esclerosis Múltiple Recurrente-Remitente/complicaciones , Adolescente , Adulto , Trastornos del Conocimiento/etiología , Trastornos del Conocimiento/patología , Progresión de la Enfermedad , Electroencefalografía , Epilepsia/patología , Femenino , Humanos , Estudios Longitudinales , Imagen por Resonancia Magnética , Masculino , Persona de Mediana Edad , Esclerosis Múltiple Recurrente-Remitente/patología , Pruebas Neuropsicológicas , Adulto JovenRESUMEN
Cerebral hydatid disease is very rare, representing only 2% of all cerebral space occupying lesions. The diagnosis is usually based on a pathognomonic CT pattern. Exceptionally, the image is atypical raising suspicion of many differential diagnoses such as intracerebral infectious, vascular lesions, or tumors. We report 2 atypical cases of cerebral hydatid cysts diagnosed in a 21, and a 24-year-old woman. The CT scan results suggest oligodendroglioma in the first case and brain abscess in the second. An MRI was helpful in the diagnosis of the 2 cases. Both patients underwent successful surgery with a good outcome. The hydatid nature of the cyst was confirmed by histology in both cases.
Asunto(s)
Infecciones Parasitarias del Sistema Nervioso Central/diagnóstico por imagen , Infecciones Parasitarias del Sistema Nervioso Central/patología , Corteza Cerebral/diagnóstico por imagen , Echinococcus/patogenicidad , Animales , Corteza Cerebral/parasitología , Corteza Cerebral/cirugía , Femenino , Humanos , Imagen por Resonancia Magnética , Tomografía Computarizada por Rayos X , Adulto JovenRESUMEN
OBJECTIVE: The present study aimed to establish a cerebral schistosomiasis model in rabbits, to provide a valuable tool for morphological analysis, clinical manifestation observation, as well as investigations into immunological reactions and pathogenesis of focal inflammatory reaction in neuroschistosomiasis (NS). METHODS: Sixty New Zealand rabbits were randomly assigned into operation, sham-operation and normal groups. Rabbits in the operation group received direct injection of dead schistosome eggs into the brain, while their counterparts in the sham-operation group received saline injection. Rabbits in the normal group received no treatment. Base on the clinical manifestations, rabbits were sacrificed on days 3, 5, 7, 10, 20, and 30 post injection, and brain samples were sectioned and stained with hematoxylin-eosin. Sections were observed under the microscope. RESULTS: The rabbits in the operation group exhibited various neurological symptoms, including anorexy, partial and general seizures, and paralysis. The morphological analysis showed several schistosome eggs in the nervous tissue on day 3 post operation, with very mild inflammation. On days 7-10 post operation, several schistosome eggs were localized in proximity to red blood cells with many neutrophilic granulocytes and eosinophilic granulocytes around them. The schistosome eggs developed into the productive granuloma stage on days 14-20 post operation. On day 30, the schistosome eggs were found to be in the healing-by-fibrosis stage, and the granuloma area was replaced by fibrillary glia through astrocytosis. The sham-operation group and the normal group showed negative results. CONCLUSION: This method might be used to establish the cerebral schistosomiasis experimental model. Several factors need to be considered in establishing this model, such as the antigenic property of eggs, the time of scarification, and the clinical manifestations.
Asunto(s)
Corteza Cerebral/patología , Corteza Cerebral/parasitología , Modelos Animales de Enfermedad , Neuroesquistosomiasis , Schistosoma japonicum/patogenicidad , Animales , Huevos/efectos adversos , Femenino , Masculino , Neuroesquistosomiasis/parasitología , Neuroesquistosomiasis/patología , Neuroesquistosomiasis/fisiopatología , Conejos , Factores de TiempoRESUMEN
CASE REPORT: We present the case of a 29 year-old man who came to the Emergency Department due to pain in the right eye. There was demonstrated a complete corneal de-epithelialisation. There was no clinical improvement after appropriate treatment, which was complicated by migraine and vomiting. The computerized tomography (CT) scan showed images suggestive of cerebral toxoplasmosis. After the complete tarsorrhaphy a restitution ad integrum was observed. CONCLUSION: The diagnosis of a neurotrophic corneal ulcer due to an affected trigeminal nerve in the context of a cerebral toxoplasmosis, tarsorrhaphy is an effective procedure to take in account in corneal epithelial defects resistant to other treatments.
Asunto(s)
Infecciones Oportunistas Relacionadas con el SIDA/complicaciones , Úlcera de la Córnea/etiología , Párpados/cirugía , Toxoplasmosis Cerebral/complicaciones , Nervio Trigémino/fisiopatología , Adulto , Antibacterianos/administración & dosificación , Antibacterianos/uso terapéutico , Cerebelo/diagnóstico por imagen , Cerebelo/parasitología , Corteza Cerebral/diagnóstico por imagen , Corteza Cerebral/parasitología , Coccidiostáticos/administración & dosificación , Coccidiostáticos/uso terapéutico , Terapia Combinada , Úlcera de la Córnea/tratamiento farmacológico , Úlcera de la Córnea/fisiopatología , Úlcera de la Córnea/cirugía , Quimioterapia Combinada , Ojo/inervación , Ganciclovir/administración & dosificación , Ganciclovir/uso terapéutico , Humanos , Masculino , Midriáticos/administración & dosificación , Midriáticos/uso terapéutico , Soluciones Oftálmicas , Tomografía Computarizada por Rayos X , Toxoplasma/aislamiento & purificación , Toxoplasmosis Cerebral/diagnóstico por imagen , Toxoplasmosis Cerebral/tratamiento farmacológicoRESUMEN
Histopathological specimen of a neurocysticercosis patient, who had been living in several endemic countries, was retrospectively analyzed for assessing the origin of the infection. Mitochondrial DNA analysis strongly suggested that the patient became infected with the parasite in Nepal at least 10 years before the onset of the disease.