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1.
Methods Mol Biol ; 2044: 51-60, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31432405

RESUMEN

The embryonic cerebrospinal fluid (eCSF) influences neuroepithelial cell behavior, affecting proliferation, differentiation, and survival. One major question to resolve in the field is to precisely describe the eCSF molecules responsible and to understand how these molecules interact in order to exert their functions. Here we describe an in vitro protocol to analyze the influence of eCSF components on neuroepithelium development.


Asunto(s)
Técnicas de Cultivo de Célula/métodos , Proteínas del Líquido Cefalorraquídeo/metabolismo , Células Neuroepiteliales/citología , Animales , Diferenciación Celular , Proliferación Celular , Células Cultivadas , Proteínas del Líquido Cefalorraquídeo/aislamiento & purificación , Proteínas del Líquido Cefalorraquídeo/fisiología , Embrión de Pollo , Inmunohistoquímica/métodos , Neurogénesis , Técnicas de Cultivo de Órganos/métodos , Tegmento Mesencefálico/citología , Tegmento Mesencefálico/embriología
2.
J Pediatr ; 161(6): 1169-71, 2012 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-22985722

RESUMEN

This prospective multicenter study of 200 patients with fever-associated status epilepticus (FSE), of whom 136 underwent a nontraumatic lumbar puncture, confirms that FSE rarely causes cerebrospinal fluid (CSF) pleocytosis. CSF glucose and protein levels were unremarkable. Temperature, age, seizure focality, and seizure duration did not affect results. CSF pleocytosis should not be attributed to FSE.


Asunto(s)
Convulsiones Febriles/líquido cefalorraquídeo , Estado Epiléptico/líquido cefalorraquídeo , Biomarcadores/líquido cefalorraquídeo , Proteínas del Líquido Cefalorraquídeo/metabolismo , Niño , Glucosa/líquido cefalorraquídeo , Humanos , Leucocitos/metabolismo , Leucocitosis/líquido cefalorraquídeo , Leucocitosis/etiología , Estudios Prospectivos , Convulsiones Febriles/fisiopatología , Punción Espinal , Estado Epiléptico/etiología
3.
Vet Clin Pathol ; 36(1): 85-8, 2007 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-17311200

RESUMEN

BACKGROUND: Cerebrospinal fluid (CSF) is produced in the cerebral ventricles through ultrafiltration of plasma and active transport mechanisms. Evaluation of proteins in CSF may provide important information about the production of immunoglobulins within the central nervous system as well as possible disturbances in the blood-brain barrier. OBJECTIVE: The objective of this study was to measure the concentration and fractions of protein in CSF samples using a membrane microconcentrator technique followed by electrophoresis, and to compare the protein fractions obtained with those in serum. METHODS: CSF samples from 3 healthy dogs and 3 dogs with canine distemper virus infection were concentrated using a membrane microconcentrator having a 0.5 to 30,000 d nominal molecular weight limit (Ultrafree, Millipore, Billerica, MA, USA). Protein concentration was determined before and after concentration. Agarose gel electrophoresis was done on concentrated CSF samples, serum, and serial dilutions of one of the CSF samples. RESULTS: Electrophoretic bands were clearly identified in densitometer tracings in CSF samples with protein concentrations as low as 1.3 g/dL. The higher CSF protein concentration in dogs with distemper was mainly the result of increased albumin concentration. CONCLUSION: The microconcentrating method used in this study enables characterization of the main protein fractions in CSF by routine electrophoresis and may be useful for interpreting the underlying cause of changes in CSF protein concentrations.


Asunto(s)
Proteínas del Líquido Cefalorraquídeo/análisis , Proteínas del Líquido Cefalorraquídeo/metabolismo , Electroforesis en Gel de Agar/métodos , Electroforesis en Gel de Agar/veterinaria , Animales , Moquillo/líquido cefalorraquídeo , Perros , Femenino , Masculino
4.
Pediatr Neurol ; 32(3): 180-3, 2005 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-15730898

RESUMEN

Predictor variables of intra-hospital lethality among infants with pyogenic meningitis due to Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae were identified using data from a follow-up study of infants with bacterial meningitis. The infants who were admitted to Couto Maia Hospital from March 1, 1997 to December 31, 1997 presenting with symptoms of bacterial meningitis were identified and included in a database. An analysis of the clinical and laboratory information was performed using EPI info 6.01b and SPSS 6.1 statistical programs. The total mortality rate was 17.1%, and the majority of deaths occurred within 48 hours of hospitalization. Factors associated most frequently with poor outcome included absence of respiratory infection, high cerebrospinal fluid protein, and compromised cranial nerves. Early identification of major risk groups is important to adopt measures to improve prognosis.


Asunto(s)
Infecciones por Haemophilus/mortalidad , Mortalidad Hospitalaria , Meningitis Bacterianas/mortalidad , Infecciones Meningocócicas/mortalidad , Infecciones Neumocócicas/mortalidad , Proteínas del Líquido Cefalorraquídeo/metabolismo , Estudios de Cohortes , Infecciones por Haemophilus/complicaciones , Infecciones por Haemophilus/metabolismo , Humanos , Lactante , Recién Nacido , Recuento de Leucocitos , Meningitis Bacterianas/complicaciones , Meningitis Bacterianas/metabolismo , Infecciones Meningocócicas/complicaciones , Infecciones Meningocócicas/metabolismo , Infecciones Neumocócicas/complicaciones , Infecciones Neumocócicas/metabolismo , Factores de Riesgo , Tasa de Supervivencia
5.
Rev Neurol ; 39(6): 564-9, 2004.
Artículo en Español | MEDLINE | ID: mdl-15467996

RESUMEN

AIM: To review the fundamental aspects of the theory of the molecular flow/ cerebrospinal flux described recently and it can explained a group of events in the physiology of the cerebrospinal fluid and the physiopathology of neurological diseases. DEVELOPMENT: This theory was based on the postulate that a decrease of the flux rate of the cerebrospinal fluid was accompanied by an increment of the protein concentration in it and in the nervous system tissue. The increment of the protein transport from the blood to the cerebrospinal fluid not require structural changes or an increase of permeability. The reibergram or Reiber's quotient diagram, with the discriminatory hyperbolic function with its theoretical basis and its clinical relevance confirm the acceptance of the present theory. This theory was based on the first and second Fick's diffusion laws The increment of the molecular diffusion is the cause of the non-linear decrease of the cerebrospinal flux rate because of the blood-cerebrospinal fluid barrier dysfunction. CONCLUSIONS: This theory explain that an increase of the albumin quotient does not means a morphologic change on the barrier structures. The change in the cerebrospinal flux rate it has been considered the principal modulator of the protein concentration in cerebrospinal fluid in pathological conditions characterized by a blood-cerebrospinal fluid barrier dysfunction.


Asunto(s)
Líquido Cefalorraquídeo/metabolismo , Modelos Biológicos , Enfermedades del Sistema Nervioso/líquido cefalorraquídeo , Albúminas/metabolismo , Fenómenos Biofísicos , Biofisica , Barrera Hematoencefálica/anatomía & histología , Barrera Hematoencefálica/fisiología , Barrera Hematoencefálica/fisiopatología , Compartimentos de Líquidos Corporales , Cauda Equina , Proteínas del Líquido Cefalorraquídeo/metabolismo , Difusión , Humanos , Enfermedades del Sistema Nervioso/fisiopatología , Permeabilidad
6.
Cell Tissue Res ; 286(1): 23-31, 1996 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-8781209

RESUMEN

Reissner's fiber (RF) has been isolated, solubilized, and used to raise polyclonal antibodies. The present investigation has been designed: (1) to obtain antibodies against RF-glycoproteins in their native form (anti-RF-BI), after irreversible denaturation by alkylation (anti-RF-A), and after alkylation and deglycosylation by using endoglycosidase F (anti-RF-DE); (2) to use these antisera for a comparative immunocytochemical study of the subcommissural organ (SCO)-RF complex; (3) to establish the molecular mass of the deglycosylated RF-glycoproteins. Anti-RF-BI reacts with the SCO of all the species investigated. Anti-RF-A and anti-RF-DE only react with bovine SCO and RF. The three antisera stain the same bands in immunoblots of extracts of bovine SCO and RF, but anti-RF-A and anti-RF-DE reveal additional bands. The epitope common to all species reacting with anti-RF-BI is thus probably conformational in nature and associated with the integrity of the disulfide bonds. The lack of antibodies against conserved sequential epitopes in anti-RF-A does not support previous assumptions on the conserved nature of the SCO secretion. After deglycosylation by using endoglycosidase F, the RF-glycoproteins present a reduction in their molecular mass ranging between 10% and 25%. The three larger compounds (450, 300, and 230 kDa) lose their affinity for Limax flavus agglutinin (affinity = sialic acid), whereas the 190-kDa compound (170 kDa after deglycosylation) keeps its affinity for this lectin suggesting that it has N-linked and O-linked carbohydrate moieties, the three larger proteins probably having only N-linked carbohydrates.


Asunto(s)
Proteínas del Líquido Cefalorraquídeo/análisis , Glicoproteínas/análisis , Glicoproteínas/inmunología , Fibras Nerviosas/química , Órgano Subcomisural/química , Alquilación , Animales , Anticuerpos Monoclonales , Especificidad de Anticuerpos , Química Encefálica/fisiología , Bovinos , Extractos Celulares/química , Extractos Celulares/inmunología , Proteínas del Líquido Cefalorraquídeo/metabolismo , Electroforesis en Gel de Poliacrilamida , Epítopos/análisis , Glicoproteínas/metabolismo , Glicosilación , Immunoblotting , Inmunohistoquímica , Peso Molecular , Médula Espinal/química
8.
J Pediatr ; 88(3): 424-6, 1976 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-1245952

RESUMEN

Persistent pleocytosis of greater than 60 white blood cells/mm3 was commonly seen in children adequately treated for bacterial meningitis. It occurred in 13 of 21 (62%) children with Hemophilus influenzae meningitis and in 2 of 9 (22%) with pneumococcal meningitis. Pleocytosis alone cannot be used as an indication of prolonging therapy; significance of persistent pleocytosis is not known.


Asunto(s)
Líquido Cefalorraquídeo/citología , Linfocitos , Meningitis Neumocócica/líquido cefalorraquídeo , Adolescente , Antibacterianos/uso terapéutico , Proteínas del Líquido Cefalorraquídeo/metabolismo , Niño , Preescolar , Humanos , Lactante , Recuento de Leucocitos , Meningitis por Haemophilus/sangre , Meningitis por Haemophilus/tratamiento farmacológico , Meningitis por Haemophilus/metabolismo , Meningitis Neumocócica/sangre , Meningitis Neumocócica/tratamiento farmacológico , Meningitis Neumocócica/metabolismo
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