RESUMEN
A new extraction method for the acetylgestagens medroxyprogesterone acetate (MPA), chloromadinone acetate and megestrol acetate, from kidney fat, has been developed. The method is a combination of matrix solid phase dispersion and solid phase extraction and is simpler and safer than previous methods, especially as it can be automated. The recovery was estimated as 59 +/- 5% (mean +/- standard deviation) for MPA. For screening purposes detection can be achieved using a commercially available enzyme immunoassay kit giving detection limits in the range of 1.0-2.0 ng g-1.
Asunto(s)
Tejido Adiposo/química , Acetato de Clormadinona/aislamiento & purificación , Acetato de Medroxiprogesterona/aislamiento & purificación , Megestrol/análogos & derivados , Mataderos , Automatización/métodos , Acetato de Clormadinona/análisis , Cromatografía Líquida de Alta Presión/métodos , Técnicas para Inmunoenzimas , Riñón , Acetato de Medroxiprogesterona/análisis , Megestrol/análisis , Megestrol/aislamiento & purificación , Acetato de Megestrol , Técnica de Dilución de Radioisótopos , Juego de Reactivos para Diagnóstico , TritioRESUMEN
Chlormadinone acetate was tested for the preparation of the "Chlormadinone Acetate Reference Standard (Control 881)". Analytical data obtained were as follows: loss on drying, 0.00%; infrared spectrum, 1740, 1715, 1658, 1604, 1587, 1256, 1244 cm-1; ultraviolet spectrum, lambda max = 284 nm; absorbance, E1cm1% (284 nm) = 539-549; optical rotation, [alpha]D20: -12.1 degrees; melting point, 214.6 degrees C; thin-layer chromatography, contaminants were not detected until 300 micrograms; high-performance liquid chromatography, one contaminant was detected. On the basis of the above results, this material was authorized as the National Institute of Hygienic Sciences Reference Standard (Control 881).