RESUMEN
Asymmetric desymmetrization through the selective reduction of one double bond of prochiral 2,5-cyclohexadienones is highly challenging. A novel method has been developed for synthesizing chiral cyclohexenones by employing an ene-reductase (Bacillus subtilis YqjM) enzyme that belongs to the OYE family. Our strategy demonstrates high substrate scope and enantioselectivity towards substrates containing all-carbon as well as heteroatom (O, N)-containing quaternary centers. The mechanistic studies (kH/D = â¼1.8) indicate that hydride transfer is probably the rate-limiting step. Mutation of several active site residues did not affect the stereochemical outcomes. This work provides a convenient way of synthesizing various enantioselective γ,γ-disubstituted cyclohexanones using enzymes.
Asunto(s)
Bacillus subtilis , Estereoisomerismo , Bacillus subtilis/enzimología , Oxidorreductasas/metabolismo , Oxidorreductasas/química , Estructura Molecular , Ciclohexenos/química , Ciclohexenos/metabolismo , Ciclohexenos/síntesis químicaRESUMEN
Human ornithine aminotransferase (hOAT), a pyridoxal 5'-phosphate (PLP)-dependent enzyme, has been shown to play an essential role in the metabolic reprogramming and progression of hepatocellular carcinoma (HCC). HCC accounts for approximately 75% of primary liver cancers and is within the top three causes of cancer death worldwide. As a result of treatment limitations, the overall 5-year survival rate for all patients with HCC is under 20%. The prevalence of HCC necessitates continued development of novel and effective treatment methods. In recent years, the therapeutic potential of selective inactivation of hOAT has been demonstrated for the treatment of HCC. Inspired by previous increased selectivity for hOAT by the expansion of the cyclopentene ring scaffold to a cyclohexene, we designed, synthesized, and evaluated a series of novel fluorinated cyclohexene analogues and identified (R)-3-amino-5,5-difluorocyclohex-1-ene-1-carboxylic acid as a time-dependent inhibitor of hOAT. Structural and mechanistic studies have elucidated the mechanism of inactivation of hOAT by 5, resulting in a PLP-inactivator adduct tightly bound to the active site of the enzyme. Intact protein mass spectrometry, 19F NMR spectroscopy, transient state kinetic studies, and X-ray crystallography were used to determine the structure of the final adduct and elucidate the mechanisms of inactivation. Interestingly, despite the highly electrophilic intermediate species conferred by fluorine and structural evidence of solvent accessibility in the hOAT active site, Lys292 and water did not participate in nucleophilic addition during the inactivation mechanism of hOAT by 5. Instead, rapid aromatization to yield the final adduct was favored.
Asunto(s)
Diseño de Fármacos , Inhibidores Enzimáticos , Ornitina-Oxo-Ácido Transaminasa , Humanos , Ornitina-Oxo-Ácido Transaminasa/metabolismo , Ornitina-Oxo-Ácido Transaminasa/química , Ornitina-Oxo-Ácido Transaminasa/antagonistas & inhibidores , Inhibidores Enzimáticos/farmacología , Inhibidores Enzimáticos/química , Inhibidores Enzimáticos/síntesis química , Ácidos Carboxílicos/química , Ácidos Carboxílicos/síntesis química , Ácidos Carboxílicos/farmacología , Ciclohexenos/química , Ciclohexenos/síntesis química , Ciclohexenos/farmacología , Ciclohexenos/metabolismo , Carcinoma Hepatocelular/tratamiento farmacológico , Carcinoma Hepatocelular/metabolismo , Cristalografía por Rayos X , Modelos MolecularesRESUMEN
The 1,4-diacyloxylation of 1,3-cyclohexadiene (CHD) affords valuable stereochemically defined scaffolds for natural product and pharmaceutical synthesis. Existing cis-selective diacyloxylation protocols require superstoichiometric quantities of benzoquinone (BQ) or MnO2 , which limit process sustainability and large-scale application. In this report, reaction development and mechanistic studies are described that overcome these limitations by pairing catalytic BQ with tert-butyl hydroperoxide as the stoichiometric oxidant. Catalytic quantities of bromide enable a switch from trans to cis diastereoselectivity. A catalyst with a 1:2 Pd:Br ratio supports high cis selectivity while retaining good rate and product yield. Further studies enable replacement of BQ with hydroquinone (HQ) as a source of cocatalyst, avoiding the handling of volatile and toxic BQ in large-scale applications.
Asunto(s)
Acetatos/química , Benzoatos/síntesis química , Ciclohexenos/síntesis química , Hidroquinonas/química , Compuestos Organometálicos/química , Catálisis , Paladio/química , EstereoisomerismoRESUMEN
Prenylated indole alkaloids featuring spirooxindole rings possess a 3R or 3S carbon stereocenter, which determines the bioactivities of these compounds. Despite the stereoselective advantages of spirooxindole biosynthesis compared with those of organic synthesis, the biocatalytic mechanism for controlling the 3R or 3S-spirooxindole formation has been elusive. Here, we report an oxygenase/semipinacolase CtdE that specifies the 3S-spirooxindole construction in the biosynthesis of 21R-citrinadin A. High-resolution X-ray crystal structures of CtdE with the substrate and cofactor, together with site-directed mutagenesis and computational studies, illustrate the catalytic mechanisms for the possible ß-face epoxidation followed by a regioselective collapse of the epoxide intermediate, which triggers semipinacol rearrangement to form the 3S-spirooxindole. Comparing CtdE with PhqK, which catalyzes the formation of the 3R-spirooxindole, we reveal an evolutionary branch of CtdE in specific 3S spirocyclization. Our study provides deeper insights into the stereoselective catalytic machinery, which is important for the biocatalysis design to synthesize spirooxindole pharmaceuticals.
Asunto(s)
Ciclohexenos/síntesis química , Ciclohexenos/metabolismo , Alcaloides Indólicos/síntesis química , Alcaloides Indólicos/metabolismo , Vías Biosintéticas/genética , Catálisis , Técnicas de Química Sintética , Compuestos Epoxi , Fermentación , Proteínas Fúngicas/genética , Modelos Moleculares , Estructura Molecular , Oxigenasas , Penicillium/genética , Penicillium/metabolismoRESUMEN
Glycoside hydrolases (GH) are a large family of hydrolytic enzymes found in all domains of life. As such, they control a plethora of normal and pathogenic biological functions. Thus, understanding selective inhibition of GH enzymes at the atomic level can lead to the identification of new classes of therapeutics. In these studies, we identified a 4-âº-glucoside of valienamine (8) as an inhibitor of Streptomyces coelicolor (Sco) GlgE1-V279S which belongs to the GH13 Carbohydrate Active EnZyme family. The results obtained from the dose-response experiments show that 8 at a concentration of 1000 µM reduced the enzyme activity of Sco GlgE1-V279S by 65%. The synthetic route to 8 and a closely related 4-âº-glucoside of validamine (7) was achieved starting from readily available D-maltose. A key step in the synthesis was a chelation-controlled addition of vinylmagnesium bromide to a maltose-derived enone intermediate. X-ray structures of both 7 and 8 in complex with Sco GlgE1-V279S were solved to resolutions of 1.75 and 1.83 Å, respectively. Structural analysis revealed the valienamine derivative 8 binds the enzyme in an E2 conformation for the cyclohexene fragment. Also, the cyclohexene fragment shows a new hydrogen-bonding contact from the pseudo-diaxial C(3)-OH to the catalytic nucleophile Asp 394 at the enzyme active site. Asp 394, in fact, forms a bidentate interaction with both the C(3)-OH and C(7)-OH of the inhibitor. In contrast, compound 7 disrupts the catalytic sidechain interaction network of Sco GlgE1-V279S via steric interactions resulting in a conformation change in Asp 394. These findings will have implications for the design other aminocarbasugar-based GH13-inhibitors and will be useful for identifying more potent and selective inhibitors.
Asunto(s)
Proteínas Bacterianas/antagonistas & inhibidores , Ciclohexenos/síntesis química , Glucósidos/síntesis química , Inhibidores de Glicósido Hidrolasas/síntesis química , Glicósido Hidrolasas/química , Hexosaminas/síntesis química , Streptomyces coelicolor/enzimología , Sustitución de Aminoácidos , Aminoácidos/química , Proteínas Bacterianas/química , Proteínas Bacterianas/genética , Conformación de Carbohidratos , Dominio Catalítico , Cristalografía por Rayos X , Ciclohexenos/farmacología , Glucósidos/farmacología , Inhibidores de Glicósido Hidrolasas/farmacología , Glicósido Hidrolasas/genética , Hexosaminas/farmacología , Maltosa/química , Modelos Moleculares , Mutación Missense , Resonancia Magnética Nuclear Biomolecular , Mutación Puntual , Estereoisomerismo , Streptomyces coelicolor/genéticaRESUMEN
Strained cyclic allenes, first discovered in 1966 by Wittig and co-workers, have recently emerged as valuable synthetic building blocks. Previous experimental investigations, and computations reported here, demonstrate that the Diels-Alder reactions of furans and pyrroles with 1,2-cyclohexadiene and oxa- and azaheterocyclic analogs proceed with endo selectivity. This endo selectivity gives the adduct with the allylic saturated carbon of the cyclic allene endo to the diene carbons. The selectivity is very general and useful in synthetic applications. Our computational study establishes the origins of this endo selectivity. We analyze the helical frontier molecular orbitals of strained cyclic allenes and show how secondary orbital and electrostatic effects influence stereoselectivity. The LUMO of carbon-3 of the allene (C-3 is not involved in primary orbital interactions) interacts in a stabilizing fashion with the HOMO of the diene in such a way that the carbon of the cyclic allene attached to C-1 favors the endo position in the transition state. The furan LUMO, allene HOMO interaction reinforces this preference. These mechanistic studies are expected to prompt the further use of long-avoided strained cyclic allenes in chemical synthesis.
Asunto(s)
Ciclohexenos/síntesis química , Reacción de Cicloadición , Ciclohexenos/química , Estructura Molecular , EstereoisomerismoRESUMEN
The Diels-Alder (DA) reaction is a cycloaddition of a conjugated diene and an alkene (dienophile) leading to the formation of a cyclohexene derivative through a concerted mechanism. As DA reactions generally proceed with a high degree of regio- and stereoselectivity, they are widely used in synthetic organic chemistry. Considering eco-conscious public and governmental movements, efforts are now directed towards the development of synthetic processes that meet environmental concerns. Artificial enzymes, which can be developed to catalyze abiotic reactions, appear to be important synthetic tools in the synthetic biology field. This review describes the different strategies used to develop protein-based artificial enzymes for DA reactions, including for in cellulo approaches.
Asunto(s)
Ciclohexenos/síntesis química , Albúmina Sérica/química , Animales , Reacción de Cicloadición , Ciclohexenos/química , Humanos , Modelos Moleculares , Estructura Molecular , PorcinosRESUMEN
BACKGROUND: Triple-negative BC is the most aggressive type of breast cancer and its lack of responsiveness to conventional therapies requires screening of new chemical entities. Anti-migratory compounds are promising to treat metastatic cancer since they inhibit one of the main steps of the metastatic cascade. Spirocyclic compounds are non-conventional structures used as building blocks for the synthesis of biologically active molecules and considered interesting structures in the search for new targets in cancer research. OBJECTIVE: Here, we evaluated the potential of eight synthetic spirocyclohexadienones as cell migration inhibitors. METHODS: The anti-migratory ability of compounds was tested by wound healing and Boyden chamber approaches. Experiments in tubulin were performed by fluorescence and tubulin polymerization techniques. Finally, compounds were submitted to cell proliferation inhibition and flow cytometry assays to explore the mechanism by which they inhibit cell migration. RESULTS: Four compounds inhibited cell migration significantly. Analogs containing the 3,4,5-trimethoxyphenil ring at R1 position were the most potent and, thus, selected for additional experiments. Tubulin polymerization and fluorescence assays highlighted a possible binding of spirocyclohexadienones in the colchicine binding site; however, these compounds did not affect the cell cycle to the same extent as colchicine. Cell proliferation was affected and, notably, the most potent analogs induced apoptosis of tumor cells, suggesting a different mechanism by which they inhibit cell migration. CONCLUSION: We presented, for the first time, a series of eight synthetic spirocyclohexadienones with the ability to inhibit TNBC cell migration. These compounds represent a new category to be explored as anticancer agents.
Asunto(s)
Antineoplásicos/farmacología , Ciclohexenos/farmacología , Compuestos de Espiro/farmacología , Neoplasias de la Mama Triple Negativas/tratamiento farmacológico , Moduladores de Tubulina/farmacología , Antineoplásicos/síntesis química , Antineoplásicos/química , Apoptosis/efectos de los fármacos , Ciclo Celular/efectos de los fármacos , Movimiento Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Células Cultivadas , Ciclohexenos/síntesis química , Ciclohexenos/química , Ensayos de Selección de Medicamentos Antitumorales , Humanos , Estructura Molecular , Polimerizacion/efectos de los fármacos , Compuestos de Espiro/síntesis química , Compuestos de Espiro/química , Neoplasias de la Mama Triple Negativas/patología , Tubulina (Proteína)/metabolismo , Moduladores de Tubulina/síntesis química , Moduladores de Tubulina/químicaRESUMEN
Gold nanoclusters and isolated gold atoms have been produced in a two-liquid phase procedure that involves a solution of gold in aqua regia and rosemary essential oil as organic layer. These gold entities have been immobilized on the ordered mesoporous silica material SBA-15 functionalized with different amounts of aminopropyl groups. The resulting materials have been characterized by XRD, N2 adsorption, chemical analysis, TGA, 29Si MAS NMR, 13C CP/MAS NMR, UV-vis spectroscopy, XPS, and STEM. The Au-containing materials retain the ordering and porosity of the pristine support. Gold content varies in the range of 0.07-0.7 wt% as a function of the specific immobilization conditions, while STEM evidences the presence of isolated gold atoms. XPS shows a shift of the Au 4f BE toward values lower than those of metallic gold. The catalytic activity in the oxidation of cyclohexene with molecular oxygen at atmospheric pressure parallels the Au content of the aminopropyl-SBA-15 supports. This activity is higher than that of analogous Au entities immobilized on SBA-15 functionalized with thiol or sulfonate groups, the activity decreasing in the order Au-NH2 > Au-SO3- > Au-SH. This behavior has been attributed to differences in the interaction strength between the functional group and the Au entities, which is optimum for the aminopropyl groups.
Asunto(s)
Ciclohexenos/química , Oro/química , Oxidación-Reducción , Catálisis , Fenómenos Químicos , Técnicas de Química Sintética , Ciclohexenos/síntesis química , Espectroscopía de Resonancia Magnética , Porosidad , Dióxido de Silicio/química , Difracción de Rayos XRESUMEN
A series of mono- and di-methylenecyclohexenone derivatives, 3a-f and 4a-f, respectively, were designed and synthesized from piperlongumine (PL) and their in vitro and in vivo pharmacological properties were evaluated. A majority of the compounds exhibited a potent antiproliferative effect on five human cancer cell lines, especially those causing breast cancer. Compound 4f showed the highest antiproliferative potency among all of the compounds, almost a 10-fold higher inhibitory potency against thioredoxin reductase (TrxR) compared with PL in cells causing breast cancer. In addition, 4f was found to increase the levels of reactive oxygen species (ROS), thus leading to more potent antiproliferative effects. More importantly, the suppression assays of migration and invasion revealed that compound 4f could reverse the epithelial-mesenchymal transition induced by the transforming growth factor ß1, and exhibit prominent anti-metastasis effects. Compound 4f also showed strong inhibition potency toward solid tumors of breast cancer in vivo. Our findings show that compound 4f is a promising therapeutic candidate in the treatment of breast cancer, which, however, needs further research to be proved.
Asunto(s)
Antineoplásicos/farmacología , Ciclohexenos/farmacología , Inhibidores Enzimáticos/farmacología , Reductasa de Tiorredoxina-Disulfuro/antagonistas & inhibidores , Animales , Antineoplásicos/síntesis química , Antineoplásicos/química , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Ciclohexenos/síntesis química , Ciclohexenos/química , Relación Dosis-Respuesta a Droga , Ensayos de Selección de Medicamentos Antitumorales , Inhibidores Enzimáticos/síntesis química , Inhibidores Enzimáticos/química , Humanos , Masculino , Neoplasias Mamarias Experimentales/tratamiento farmacológico , Neoplasias Mamarias Experimentales/metabolismo , Neoplasias Mamarias Experimentales/patología , Ratones , Ratones Endogámicos BALB C , Ratones Desnudos , Estructura Molecular , Especies Reactivas de Oxígeno/análisis , Especies Reactivas de Oxígeno/metabolismo , Relación Estructura-Actividad , Reductasa de Tiorredoxina-Disulfuro/metabolismo , Células Tumorales CultivadasRESUMEN
The reactions of electron-rich organosilicon compounds 1,4-bis(trimethylsilyl)-1,4-diaza-2,5-cyclohexadiene (1), 2,3,5,6-tetramethyl-1,4-bis(trimethylsilyl)-1,4-diaza-2,5-cyclohexadiene (2), and 1,1'-bis(trimethylsilyl)-1,1'-dihydro-4,4'-bipyridine (12) with B-amino and B-aryl dihaloboranes afforded a series of novel B=N-bond-containing compounds 3-11 and 13. The B=N rotational barriers of 7 (>71.56 kJ/mol), 10 (58.79 kJ/mol), and 13 (58.65 kJ/mol) were determined by variable-temperature 1H-NMR spectroscopy, thus reflecting different degrees of B=N double bond character in the corresponding compounds. In addition, ring external olefin isomers 11 were obtained by a reaction between 2 and DurBBr2. All obtained B=N-containing products were characterized by multinuclear NMR spectroscopy. Compounds 5, 9, 10a, 11, and 13a were also characterized by single-crystal X-ray diffraction analysis.
Asunto(s)
Ciclohexenos/química , Compuestos Heterocíclicos/química , Compuestos de Organosilicio/química , Cristalografía por Rayos X , Ciclohexenos/síntesis química , Electrones , Espectroscopía de Resonancia Magnética , Modelos Moleculares , TemperaturaRESUMEN
The hydrogen isotopes deuterium (D) and tritium (T) have become essential tools in chemistry, biology and medicine1. Beyond their widespread use in spectroscopy, mass spectrometry and mechanistic and pharmacokinetic studies, there has been considerable interest in incorporating deuterium into drug molecules1. Deutetrabenazine, a deuterated drug that is promising for the treatment of Huntington's disease2, was recently approved by the United States' Food and Drug Administration. The deuterium kinetic isotope effect, which compares the rate of a chemical reaction for a compound with that for its deuterated counterpart, can be substantial1,3,4. The strategic replacement of hydrogen with deuterium can affect both the rate of metabolism and the distribution of metabolites for a compound5, improving the efficacy and safety of a drug. The pharmacokinetics of a deuterated compound depends on the location(s) of deuterium. Although methods are available for deuterium incorporation at both early and late stages of the synthesis of a drug6,7, these processes are often unselective and the stereoisotopic purity can be difficult to measure7,8. Here we describe the preparation of stereoselectively deuterated building blocks for pharmaceutical research. As a proof of concept, we demonstrate a four-step conversion of benzene to cyclohexene with varying degrees of deuterium incorporation, via binding to a tungsten complex. Using different combinations of deuterated and proteated acid and hydride reagents, the deuterated positions on the cyclohexene ring can be controlled precisely. In total, 52 unique stereoisotopomers of cyclohexene are available, in the form of ten different isotopologues. This concept can be extended to prepare discrete stereoisotopomers of functionalized cyclohexenes. Such systematic methods for the preparation of pharmacologically active compounds as discrete stereoisotopomers could improve the pharmacological and toxicological properties of drugs and provide mechanistic information related to their distribution and metabolism in the body.
Asunto(s)
Benceno/química , Técnicas de Química Sintética , Ciclohexenos/química , Ciclohexenos/síntesis química , Deuterio/química , Preparaciones Farmacéuticas/química , Preparaciones Farmacéuticas/síntesis química , Bases de Datos de Compuestos Químicos , Cinética , Estructura Molecular , Estereoisomerismo , Tetrabenazina/análogos & derivados , Tetrabenazina/síntesis química , Tetrabenazina/química , Tungsteno/químicaRESUMEN
A photocatalytic system for the dearomative hydroarylation of benzene derivatives has been developed. Using a combination of an organic photoredox catalyst and an amine reductant, this process operates through a reductive radical-polar crossover mechanism where aryl halide reduction triggers a regioselective radical cyclization event, followed by anion formation and quenching to produce a range of complex spirocyclic cyclohexadienes. This light-driven protocol functions at room temperature in a green solvent system (aq. MeCN) without the need for precious metal-based catalysts or reagents or the generation of stoichiometric metal byproducts.
Asunto(s)
Derivados del Benceno/química , Ciclohexenos/síntesis química , Compuestos de Espiro/síntesis química , Ciclohexenos/química , Radicales Libres/química , Estructura Molecular , Oxidación-Reducción , Procesos Fotoquímicos , Compuestos de Espiro/químicaRESUMEN
Transient strained cyclic intermediates have become valuable intermediates in modern synthetic chemistry. Although silyl triflate precursors to strained intermediates are most often employed, the instability of some silyl triflates warrants the development of alternative precursors. We report the syntheses of silyl tosylate precursors to cyclohexyne, 1,2-cyclohexadiene, and 1,2-cycloheptadiene. The resultant strained intermediates undergo trapping in situ to give cycloaddition products. Additionally, the results of competition experiments between silyl triflates and silyl tosylates are reported.
Asunto(s)
Cicloheptanos/síntesis química , Ciclohexenos/síntesis química , Silanos/química , Compuestos de Tosilo/química , Reacción de Cicloadición , Cicloheptanos/química , Ciclohexenos/química , Estructura Molecular , EstereoisomerismoRESUMEN
Six platinum(IV) compounds derived from an oxaliplatin analogue containing the unsaturated cyclic diamine trans-1,2-diamino-4-cyclohexene (DACHEX), in place of the 1,2-diaminocyclohexane, and a range of axial ligands, were synthesized and characterized. The derivatives with at least one axial chlorido ligand demonstrated solvent-assisted photoreduction. The electrochemical redox behavior was investigated by cyclic voltammetry; all compounds showed reduction potentials suitable for activation in vivo. X-ray photoelectron spectroscopy (XPS) data indicated an X-ray-induced surface reduction of the Pt(IV) substrates, which correlates with the reduction potentials measured by cyclic voltammetry. The cytotoxic activity was assessed in vitro on a panel of human cancer cell lines, also including oxaliplatin-resistant cancer cells, and compared with that of the reference compounds cisplatin and oxaliplatin; all IC50 values were remarkably lower than those elicited by cisplatin and somewhat lower than those of oxaliplatin. Compared to the other Pt(IV) compounds of the series, the bis-benzoate derivative was by far (5-8 times) the most cytotoxic showing that low reduction potential and high lipophilicity are essential for good cytotoxicity. Interestingly, all the complexes proved to be more active than cisplatin and oxaliplatin even in three-dimensional spheroids of A431 human cervical cancer cells.
Asunto(s)
Antineoplásicos/química , Ciclohexenos/química , Compuestos Organoplatinos/química , Oxaliplatino/análogos & derivados , Profármacos/química , Antineoplásicos/síntesis química , Antineoplásicos/farmacología , Línea Celular Tumoral , Cisplatino/química , Cisplatino/farmacología , Ciclohexenos/síntesis química , Resistencia a Antineoplásicos , Ensayos de Selección de Medicamentos Antitumorales , Humanos , Ligandos , Neoplasias/tratamiento farmacológico , Compuestos Organoplatinos/síntesis química , Compuestos Organoplatinos/farmacología , Oxaliplatino/química , Oxaliplatino/farmacología , Profármacos/síntesis química , Profármacos/farmacologíaRESUMEN
Microbial arene oxidation of benzoic acid with Ralstonia eutropha B9 provides a chiral highly functionalized cyclohexadiene, suitable for further structural diversification. Subjecting this scaffold to a Pd-catalyzed Heck reaction effects a regio- and stereoselective arylation of the cyclohexadiene ring, with 1,3-chirality transfer of stereogenic information installed in the microbial arene oxidation. Quantum chemical calculations explain the selectivity both by a kinetic preference for the observed arylation position and by reversible carbopalladation in competing positions. Further product transformation allowed the formation of a tricyclic ketone possessing four stereogenic centers. This demonstrates the capability of the method to introduce stereochemical complexity from planar nonchiral benzoic acid in just a few steps.
Asunto(s)
Cupriavidus necator/metabolismo , Ciclohexenos/síntesis química , Paladio/química , Benzoatos/química , Catálisis , Cupriavidus necator/química , Yodobencenos/química , Oxidación-Reducción , EstereoisomerismoRESUMEN
Polysubstituted cyclohexenes bearing 1,3 (meta) substitution patterns are challenging to access using the Diels-Alder reaction (the ortho-para rule). Here, we report a cobalt-catalyzed reductive [5 + 1]-cycloaddition between a vinylcyclopropane and a vinylidene to provide methylenecyclohexenes bearing all-meta relationships. Vinylidene equivalents are generated from 1,1-dichloroalkenes using Zn as a stoichiometric reductant. Experimental observations are consistent with a mechanism involving a cobaltacyclobutane formed from a [2 + 2]-cycloaddition between a cobalt vinylidene and a vinylcyclopropane.
Asunto(s)
Ciclohexenos/síntesis química , Ciclopropanos/química , Compuestos de Vinilo/química , Catálisis , Cobalto/química , Complejos de Coordinación/química , Reacción de Cicloadición , Modelos QuímicosRESUMEN
Engineered enzyme cascades offer powerful tools to convert renewable resources into value-added products. Man-made catalysts give access to new-to-nature reactivities that may complement the enzyme's repertoire. Their mutual incompatibility, however, challenges their integration into concurrent chemo-enzymatic cascades. Herein we show that compartmentalization of complex enzyme cascades within E. coli whole cells enables the simultaneous use of a metathesis catalyst, thus allowing the sustainable one-pot production of cycloalkenes from oleic acid. Cycloheptene is produced from oleic acid via a concurrent enzymatic oxidative decarboxylation and ring-closing metathesis. Cyclohexene and cyclopentene are produced from oleic acid via either a six- or eight-step enzyme cascade involving hydration, oxidation, hydrolysis and decarboxylation, followed by ring-closing metathesis. Integration of an upstream hydrolase enables the usage of olive oil as the substrate for the production of cycloalkenes. This work highlights the potential of integrating organometallic catalysis with whole-cell enzyme cascades of high complexity to enable sustainable chemistry.
Asunto(s)
Biocatálisis , Cicloparafinas/síntesis química , Ácidos Dicarboxílicos , Escherichia coli , Ácido Oléico , Aceite de Oliva , Ciclohexenos/síntesis química , Ciclopentanos/síntesis química , Descarboxilación , Hidrólisis , Compuestos Organometálicos , Oxidación-Reducción , Biología SintéticaRESUMEN
A facile and diversity-oriented synthetic strategy toward aminocyclitol natural products from inexpensive C2-symmetric l-tartaric acid was developed. The pivotal epoxide was used as a common intermediate to accomplish eight diverse target molecules in six to eleven steps. Various allyl-amine-type conduramines were synthesized in a diastereoselective manner. Heck arylation was explored to construct a phenanthridone ring in a concise synthesis of (+)-lycoricidine. In addition, a highly efficient formal synthesis of (-)-laminitol was developed.
Asunto(s)
Alcaloides de Amaryllidaceae/síntesis química , Aminas/síntesis química , Ciclohexenos/síntesis química , Inositol/análogos & derivados , Fenantridinas/síntesis química , Fenoles/síntesis química , Alcaloides de Amaryllidaceae/química , Aminas/química , Ciclohexenos/química , Inositol/síntesis química , Inositol/química , Estructura Molecular , Fenantridinas/química , Fenoles/química , EstereoisomerismoRESUMEN
Silyl triflate precursors to cyclic alkynes and allenes serve as valuable synthetic building blocks. We report a concise and scalable synthetic approach to prepare the silyl triflate precursors to cyclohexyne and 1,2-cyclohexadiene. The strategy involves a retro-Brook rearrangement of an easily accessible cyclohexanone derivative, followed by triflation protocols. This simple, yet controlled, method should enable the further study of strained alkynes and allenes in chemical synthesis.