RESUMEN
The auditory ossicles amplify and transmit sound from the environment to the inner ear. The distribution of bone mineral density is crucial for the proper functioning of sound transmission as the ossicles are suspended in an air-filled chamber. However, little is known about the distribution of bone mineral density along the human ossicular chain and within individual ossicles. To investigate this, we analyzed fresh-frozen human specimens using synchrotron-based phase-contrast microtomography. In addition, we analyzed the volume and porosity of the ossicles. The porosity for the auditory ossicles lies, on average, between 1.92% and 9.85%. The average volume for the mallei is 13.85 ± 2.15 mm3, for the incudes 17.62 ± 4.05 mm3 and 1.24 ± 0.29 mm3 for the stapedes. The bone density distribution showed a similar pattern through all samples. In particular, we found high bone mineralization spots on the anterior crus of the stapes, its footplate, and along areas that are crucial for the transmission of sound. We could also see a correlation between low bone mineral density and holey areas where the bone is only very thin or missing. Our study identified a similar pattern of bone density distribution within all samples: regions exposed to lower forces generally show higher bone density. Further, we observed that the stapes shows high bone mineral density along the anterior crus and its footplate, which may indicate its importance in transmitting sound waves to the inner ear.
Asunto(s)
Densidad Ósea , Osículos del Oído , Sincrotrones , Microtomografía por Rayos X , Humanos , Osículos del Oído/diagnóstico por imagen , Osículos del Oído/fisiología , Osículos del Oído/anatomía & histología , Microtomografía por Rayos X/métodos , Porosidad , Femenino , Anciano , Masculino , Persona de Mediana Edad , Estribo/fisiología , Estribo/diagnóstico por imagenRESUMEN
The time delay and/or malfunctioning of the Eustachian tube may cause pressure differences across the tympanic membrane, resulting in quasi-static movements of the middle-ear ossicles. While quasi-static displacements of the human middle-ear ossicles have been measured one- or two-dimensionally in previous studies, this study presents an approach to trace three-dimensional movements of the human middle-ear ossicles under static pressure loads in the ear canal (EC). The three-dimensional quasi-static movements of the middle-ear ossicles were measured using a custom-made stereo camera system. Two cameras were assembled with a relative angle of 7° and then mounted onto a robot arm. Red fluorescent beads of a 106-125 µm diameter were placed on the middle-ear ossicles, and quasi-static position changes of the fluorescent beads under static pressure loads were traced by the stereo camera system. All the position changes of the ossicles were registered to the anatomical intrinsic frame based on the stapes footplate, which was obtained from µ-CT imaging. Under negative ear-canal pressures, a rotational movement around the anterior-posterior axis was dominant for the malleus-incus complex, with small relative movements between the two ossicles. The stapes showed translation toward the lateral direction and rotation around the long axis of the stapes footplate. Under positive EC pressures, relative motion between the malleus and the incus at the IMJ became larger, reducing movements of the incus and stapes considerably and thus performing a protection function for the inner-ear structures. Three-dimensional tracing of the middle-ear ossicular chain provides a better understanding of the protection function of the human middle ear under static pressured loads as immediate responses without time delay.
Asunto(s)
Osículos del Oído , Oído Medio , Humanos , Oído Medio/fisiología , Osículos del Oído/fisiología , Yunque/fisiología , Estribo/fisiología , RotaciónRESUMEN
To assess the effects of ossicular chain malformations on the performance of bone conduction hearing aids, a human ear finite-element model that includes an ear canal, a middle ear, and a spiral cochlea incorporating the third windows was established. This finite element model was built based on micro-computed tomography scanning and reverse modelling techniques, and the reliability of the finite element model was verified by comparison with reported experimental data. Based on this model, two main types of ossicular chain malformations, i.e., the incudostapedial disconnection and the ossicles fixation, were simulated, and their influences on bone conduction were analyzed by comparing the trans-cochlear-partition differential pressures. The results indicate that the incudostapedial disconnection mainly deteriorates the bone conduction response at mid frequencies. The stapes fixation has the largest effect among the ossicles fixation with the bone conduction stimulation, which also mainly decreases the mid-frequency response of the bone conduction, especially at 2 kHz. As the speech intelligibility has the most important frequency range at the range between 1 kHz and 2.5 kHz, the mid-frequency deterioration caused by ossicular chain malformations should be compensated in optimizing the design of the bone conduction hearing aids. For treating patients with the ossicular chain malformations, especially for the patients who suffer from the stapes fixation, the output of bone conduction hearing aids' actuator in the middle frequency band should be improved.
Asunto(s)
Conducción Ósea/fisiología , Osículos del Oído/anomalías , Oído Medio/anomalías , Audífonos , Cóclea/anomalías , Osículos del Oído/fisiología , Oído Medio/fisiología , Análisis de Elementos Finitos , Humanos , Modelos Anatómicos , Modelos Teóricos , Presión , Diseño de Prótesis , Reproducibilidad de los Resultados , Estribo/anomalías , Estribo/fisiología , Microtomografía por Rayos XRESUMEN
The sheep middle ear has been used in training to prepare physicians to perform surgeries and to test new ways of surgical access. This study aimed to (1) collect anatomical data and inertial properties of the sheep middle-ear ossicles and (2) explore effects of these features on sound transmission, in comparison to those of the human. Characteristic dimensions and inertial properties of the middle-ear ossicles of White-Alpine sheep (n = 11) were measured from high-resolution micro-CT data, and were assessed in comparison with the corresponding values of the human middle ear. The sheep middle-ear ossicles differed from those of human in several ways: anteroinferior orientation of the malleus handle, relatively small size of the incus with a relatively short distance to the lenticular process, a large area of the articular surfaces at the incudostapedial joint, and a relatively small moment of inertia along the anterior-posterior axis. Analysis in this study suggests that structure and orientation of the middle-ear ossicles in the sheep are conducive to an increase in the hinge-like ossicular-lever-action around the anterior-posterior axis. Considering the substantial anatomical differences, outcomes of middle-ear surgeries would presumably be difficult to assess from experiments using the sheep middle ear.
Asunto(s)
Osículos del Oído/anatomía & histología , Osículos del Oído/fisiología , Oído Medio/anatomía & histología , Oído Medio/fisiología , Ovinos/anatomía & histología , Animales , Audición/fisiología , Humanos , Yunque/anatomía & histología , Yunque/fisiología , Martillo/anatomía & histología , Martillo/fisiología , Ovinos/fisiologíaRESUMEN
The ossicular joints of the middle ear can significantly affect middle-ear function, particularly under conditions such as high-intensity sound pressures or high quasi-static pressures. Experimental investigations of the mechanical behaviour of the human incudostapedial joint have shown strong non-linearity and asymmetry in tension and compression tests, but some previous finite-element models of the joint have had difficulty replicating such behaviour. In this paper, we present a finite-element model of the joint that can match the asymmetry and non-linearity well without using different model structures or parameters in tension and compression. The model includes some of the detailed structures of the joint seen in histological sections. The material properties are found from the literature when available, but some parameters are calculated by fitting the model to experimental data from tension, compression and relaxation tests. The model can predict the hysteresis loops of loading and unloading curves. A sensitivity analysis for various parameters shows that the geometrical parameters have substantial effects on the joint mechanical behaviour. While the joint capsule affects the tension curve more, the cartilage layers affect the compression curve more.
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Osículos del Oído/fisiología , Modelos Biológicos , Elasticidad , Análisis de Elementos Finitos , Humanos , ViscosidadRESUMEN
In modern Cetacea, the ear bone complex comprises the tympanic and periotic bones forming the tympano-periotic complex (TPC), differing from temporal bone complexes of other mammals in form, construction, position, and possibly function. To elucidate its functioning in sound transmission, we studied the vibration response of 32 pairs of formaldehyde-glutaraldehyde-fixed TPCs of Globicephala macrorhynchus, the short-finned pilot whale (legally obtained in Taiji, Japan). A piezoelectric-crystal-based vibrator was surgically attached to a location on the cochlea near the exit of the acoustic nerve. The crystal delivered vibrational pulses through continuous sweeps from 5 to 50â¯kHz. The vibration response was measured as a function of frequency by Laser Doppler Vibrometry at five points on the TPC. The aim of the experiment was to clarify how the vibration amplitudes produced by different frequencies are distributed on the TPC. At the lowest frequencies (<12â¯kHz), no clear differential pattern emerged. At higher frequencies the anterolateral lip of the TP responded most sensitively with the highest displacement amplitudes, and response amplitudes decreased in orderly fashion towards the posterior part of the TPC. We propose that this works as a lever: high-frequency sounds are most sensitively received and cause the largest vibration amplitudes at the anterior part of the TP, driving movements with lower amplitude but greater force near the posteriorly located contact to the ossicular chain, which transmits the movements into the inner ear. Although force (pressure) amplification is not needed for impedance matching in water, it may be useful for driving the stiffly connected ossicles at the high frequencies used in echolocation.
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Osículos del Oído/fisiología , Ecolocación , Audición , Mecanotransducción Celular , Sonido , Membrana Timpánica/fisiología , Calderón/fisiología , Factores de Edad , Animales , Osículos del Oído/anatomía & histología , Movimiento (Física) , Presión , Membrana Timpánica/anatomía & histología , Vibración , Calderón/anatomía & histologíaRESUMEN
Background: Carbon (δ13C) and nitrogen (δ15N) isotope ratios of collagen from teeth and bone are used to study human nutrition and health. As bones are constantly remodelling throughout life, isotopic values of bone collagen represent an average of several years. In contrast, human teeth do not remodel and their primary dentine contains only the isotopic data from the time of formation. In contrast to all other bones, human auditory ossicles also appear not to remodel. As they develop in utero and finish formation in the first 2 years of life, their collagen should also represent isotopic values of these two relatively short periods.Aim: By comparing δ13C and δ15N data from ossicles and incremental dentine, this study aims to investigate how two developmental periods of the ossicles, in utero and the first 2 years of life, reflect in collagen obtained from the ossicles.Subject and methods: Ossicle and tooth samples of 12 individuals aged 0.5 ± 0.4 years to 13 ± 1 years from the nineteenth century St. Peter's burial ground in Blackburn were collected and processed to obtain bulk bone and incremental dentine collagen which was measured for δ13C and δ15N.Results: Averaged δ13C and δ15N of ossicles are lower when compared to every age group except after 3 years of age. Average offset between ossicles and dentine of different groups ranges from 0.4-0.9 for δ13C and from 0.3-0.9 for δ15N, with highest counterbalance at birth and after the first 5 months after birth.Conclusions: There appears to be a systematic offset between the dentine and ossicle data. It seems that the second phase of development does not influence the isotopic values of collagen significantly and the data we are obtaining from ossicles represents the in utero period.
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Colágeno/fisiología , Osículos del Oído/fisiología , Salud del Lactante , Salud Materna , Adolescente , Biomarcadores , Isótopos de Carbono/análisis , Niño , Preescolar , Inglaterra , Femenino , Humanos , Lactante , Recién Nacido , Radioisótopos de Nitrógeno/análisis , EmbarazoRESUMEN
Dynamic pressure at the tympanic membrane is transformed and subsequently transferred through the ossicular chain in the form of forces and moments. The forces are primarily transferred to the inner ear. They are transferred partly to the stapedial annular ligament which exhibits non-linear behavior and stiffens for larger static forces. In unventilated middle ears, static pressure is additionally transferred to the ossicles. The purpose of this study was to measure the force inside the ossicular chain as a physiological parameter. We determined the forces which act for dynamic sound transmission and for static load on the ossicular chain. The study is the first one which introduces these forces. The static forces have direct impact on clinically relevant questions for middle ear reconstructions with passive or active prosthesis. The dynamic forces have an impact on the development of middle ear sensors. Quasi-static forces in the incudostapedial joint (ISJ) gap were measured with two different sensor types in 17 temporal bones. The sensing elements, a single crystal piezo and a strain gauge element for validation, were bonded to a thin flexible titanium plate and encapsulated in a titanium housing to allow the acquisition of the applied force signal inside the ossicular chain. Dynamic forces were measured in 11 temporal bones with the piezo sensor. We measured a static force of 23â¯mN in the ISJ after sensor insertion. The mean force for dynamic physiological acoustic excitation from 250â¯Hz to 6â¯kHz was 26⯵N/Pa. If the tympanic membrane is loaded with a static pressure, the static force in the ISJ increases up to 1â¯N for a maximum static pressure load scenario of 30â¯kPa.
Asunto(s)
Osículos del Oído/fisiología , Audición , Articulaciones/fisiología , Membrana Timpánica/fisiología , Diseño de Equipo , Análisis de Elementos Finitos , Humanos , Modelos Teóricos , Movimiento (Física) , Presión , Sonido , Estrés Mecánico , Transductores de PresiónRESUMEN
In the single-ossicle ear of chickens, the quasi-static displacement of the umbo shows great asymmetry; umbo displacements are much larger for negative than for positive pressure in the middle ear, which is opposite to the typical asymmetry observed in mammal ears. To better understand this behavior, a finite-element model was created of the static response of the chicken middle ear. The role of flexibility of the extracolumella in the model was investigated, and the potential effect of the outward orientation of the tympanic-membrane cone was studied by building two adapted models with a flat membrane and an inverted conical membrane. It is found that the extracolumella must be made of flexible material to explain the large inward displacements of the umbo, and that displacements of the footplate are much smaller due to bending of the flexible extracolumella. However, increasing extracolumellar stiffness mostly reduces umbo displacement rather than increasing footplate displacement. The results suggest that the inverted orientation of the membrane cone is responsible for the change in asymmetry of the umbo displacement curve. The asymmetry of the footplate displacement curve in the normal model is smaller, but increases towards positive middle-ear pressure in the case of a flat or inverted membrane geometry.
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Pollos/fisiología , Osículos del Oído/fisiología , Oído Medio/fisiología , Audición , Modelos Teóricos , Membrana Timpánica/fisiología , Animales , Pollos/anatomía & histología , Simulación por Computador , Osículos del Oído/anatomía & histología , Oído Medio/anatomía & histología , Análisis de Elementos Finitos , Movimiento (Física) , Presión , Sonido , Membrana Timpánica/anatomía & histología , VibraciónRESUMEN
OBJECTIVES: Active middle ear implants (AMEI) have been used to treat hearing loss in patients for whom conventional hearing aids are unsuccessful for varied biologic or personal reasons. Several studies have discussed feedback as a potential complication of AMEI usage, though the feedback pathway is not well understood. While reverse propagation of an acoustic signal through the ossicular chain and tympanic membrane constitutes an air-conducted source of feedback, the implanted nature of the device microphone near the mastoid cortex suggests that bone conduction pathways may potentially be another significant factor. This study examines the relative contributions of potential sources of feedback during stimulation with an AMEI. DESIGN: Four fresh-frozen, hemi-sectioned, human cadaver specimens were prepared with a mastoid antrostomy and atticotomy to visualize the posterior incus body. A Carina active middle ear implant actuator (Cochlear Ltd., Boulder, CO) was coupled to the incus by two means: (1) a stereotactic arm mounted independently of the specimen and (2) a fixation bracket anchored directly to the mastoid cortical bone. The actuator was driven with pure-tone frequencies in 1/4 octave steps from 500 to 6000 Hz. Acoustic sound intensity in the ear canal was measured with a probe tube microphone (Bruel & Kjær, Nærum, Denmark). Bone-conducted vibration was quantified with a single-axis laser Doppler vibrometer (Polytec Inc., Irvine, CA) from both a piece of reflective tape placed on the skin overlying the mastoid and a bone-anchored titanium screw and pedestal (Cochlear Ltd., Centennial, CO) implanted in the cortical mastoid bone. RESULTS: Microphone measurements revealed ear-canal pressures of 60-89 dB SPL, peaking in the frequency range below 2 kHz. Peak LDV measurements were greatest on the mastoid bone (0.32-0.79 mm/s with mounting bracket and 0.21-0.36 mm/s with the stereotactic suspension); peak measurements on the skin ranged from 0.05 to 0.15 mm/s with the bracket and 0.03 to 0.13 mm/s with stereotactic suspension. CONCLUSION: AMEI produce both air- and bone-conducted signals of adequate strength to be detected by the implanted device microphone, potentially resulting in reamplification. Understanding the relative contribution of these sources may play an important role in the development of targeted mitigation algorithms, as well as surgical techniques emphasizing acoustic isolation.
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Conducción Ósea/fisiología , Osículos del Oído/fisiología , Audífonos , Apófisis Mastoides/fisiología , Prótesis Osicular , Membrana Timpánica/fisiología , Cadáver , Retroalimentación , Pérdida Auditiva/rehabilitación , Humanos , Cooperación del Paciente , SonidoRESUMEN
Human ear ossicles are essential for normal sound conduction from the external environment to the inner ear. These bones are subjected to high biomechanical loads due to the sustained vibrations which occur with reception of sound. It is expected that the bones would undergo a significant amount of remodeling and change in bone mineral density during the lifespan of an individual. Therefore, the aim of the study was to evaluate the possible changes in bone mineral density of regions of the ossicular chain during postnatal life. Forty four left and 36 right sets of human ear ossicles, ranging from a sub-adult age group to a mature adult age group, were sourced from the School of Anatomical Sciences, University of Witwatersrand. The ear ossicles were scanned using a micro-focus CT X-ray. A three-dimensional reconstruction of each ossicle was created from the CT scan. Bone mineral density was then determined at specific sites on the ossicles. There was no statistically significant variation found in the bone mineral density in relation to the age of the specimens. However, the handle of the malleus, the incudo-stapedial joint and the insertion site for the tendon of stapedius had lower bone mineral densities when compared to adjacent articulation and nonattachment sites on the ossicular chain. This is possibly due to biomechanical stress in response to sound conduction rather than ageing. Lower bone mineral density may be indicative of regions that experience the highest biomechanical force, thus, resulting in increased remodeling. Clin. Anat. 31:1158-1166, 2018. © 2018 Wiley Periodicals, Inc.
Asunto(s)
Densidad Ósea , Osículos del Oído/diagnóstico por imagen , Microtomografía por Rayos X/métodos , Fenómenos Biomecánicos , Cadáver , Estudios Transversales , Osículos del Oído/anatomía & histología , Osículos del Oído/fisiología , Humanos , Imagenología TridimensionalRESUMEN
The role of the ossicular joints in the mammalian middle ear is still debated. This work tests the hypothesis that the two synovial joints filter potentially damaging impulsive stimuli by transforming both the peak amplitude and width of these impulses before they reach the cochlea. The three-dimensional (3D) velocity along the ossicular chain in unaltered cadaveric human temporal bones (N = 9), stimulated with acoustic impulses, is measured in the time domain using a Polytec (Waldbronn, Germany) CLV-3D laser Doppler vibrometer. The measurements are repeated after fusing one or both of the ossicular joints with dental cement. Sound transmission is characterized by measuring the amplitude, width, and delay of the impulsive velocity profile as it travels from the eardrum to the cochlea. On average, fusing both ossicular joints causes the stapes velocity amplitude and width to change by a factor of 1.77 (p = 0.0057) and 0.78 (p = 0.011), respectively. Fusing just the incudomalleolar joint has a larger effect on amplitude (a factor of 2.37), while fusing just the incudostapedial joint decreases the stapes velocity on average. The 3D motion of the ossicles is altered by fusing the joints. Finally, the ability of current computational models to predict this behavior is also evaluated.
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Estimulación Acústica/métodos , Osículos del Oído/fisiología , Audición , Articulaciones/fisiología , Adulto , Anciano , Fenómenos Biomecánicos , Cadáver , Simulación por Computador , Osículos del Oído/anatomía & histología , Femenino , Humanos , Articulaciones/anatomía & histología , Flujometría por Láser-Doppler , Masculino , Persona de Mediana Edad , Modelos Teóricos , Movimiento (Física) , Rango del Movimiento Articular , Sonido , Factores de Tiempo , VibraciónRESUMEN
Although most birds encounter large pressure variations during flight, motion of the middle ear components as a result of changing ambient pressure are not well known or described. In the present study, motion of the columella footplate and tympanic membrane (extrastapedius) in domestic chickens (Gallus gallus domesticus) under quasi-static pressure conditions are provided. Micro-CT scans were made of cadaveric heads of chickens under positive (0.25â¯kPa, 0.5â¯kPa, 1â¯kPa, and 1.5â¯kPa) and negative (-0.25â¯kPa, -0.5â¯kPa, -1â¯kPa, and -1.5â¯kPa) middle ear pressure. Both extrastapedius and columella footplate displacements show a non-linear S-shaped curve as a function of pressure indicating non-linear response characteristics of the middle ear components. The S-curve is also seen in mammals, but unlike in mammals, the lateral piston-like displacement of both the columella footplate and extrastapedius, which is caused by an increased middle ear pressure are smaller than the medial piston-like displacements, caused by a decreased middle ear pressure of the same magnitude. Columella footplate piston displacements are always smaller than the extrastapedius piston displacements, indicating the flexibility of the extracolumella. The cone-shape of the avian tympanic membrane with inverted apex in comparison to the mammalian tympanic membrane can cause the inverted shape of the pressure response curve.
Asunto(s)
Osículos del Oído/fisiología , Audición , Mecanotransducción Celular , Sonido , Membrana Timpánica/fisiología , Animales , Pollos , Osículos del Oído/diagnóstico por imagen , Modelos Biológicos , Movimiento (Física) , Dinámicas no Lineales , Presión , Factores de Tiempo , Membrana Timpánica/diagnóstico por imagen , Microtomografía por Rayos XRESUMEN
The auditory ossicles-malleus, incus and stapes-are the smallest bones in mammalian bodies and enable stable sound transmission to the inner ear. Sperm whales are one of the deepest diving aquatic mammals that produce and perceive sounds with extreme loudness greater than 180 dB and frequencies higher than 30 kHz. Therefore, it is of major interest to decipher the microstructural basis for these unparalleled hearing abilities. Using a suite of high-resolution imaging techniques, we reveal that auditory ossicles of sperm whales are highly functional, featuring an ultra-high matrix mineralization that is higher than their teeth. On a micro-morphological and cellular level, this was associated with osteonal structures and osteocyte lacunar occlusions through calcified nanospherites (i.e. micropetrosis), while the bones were characterized by a higher hardness compared to a vertebral bone of the same animals as well as to human auditory ossicles. We propose that the ultra-high mineralization facilitates the unique hearing ability of sperm whales. High matrix mineralization represents an evolutionary conserved or convergent adaptation to middle ear sound transmission.
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Calcificación Fisiológica , Osículos del Oído/fisiología , Audición/fisiología , Cachalote/fisiología , Animales , Presión , SonidoRESUMEN
OBJECTIVES: One reason for insufficient hearing improvement with a distinct air-bone gap after ossiculoplasty with implantation of partial or total ossicular replacement prostheses can be the dislocation or minimal shifting of the prosthesis. The aim of this study was the simulation of common clinical borderline situations with minimal shifting of the prosthesis in temporal bone specimens after ossiculoplasty. It was furthermore the goal to identify these specific situations through imaging by cone beam computed tomography (cbCT) and direct visual inspection using the operation microscope. Additionally, the functional status was evaluated using laser-Doppler vibrometry (LDV). DESIGN: We used a total of four temporal bone specimens for this study. A reconstruction with a partial ossicular replacement prostheses was performed in three specimens and with a total ossicular replacement prostheses in one specimen, with good initial acoustic properties. Subsequently, one specific type of prosthesis failure was simulated in each specimen, respectively, by minimally shifting, tilting, or bending the prostheses from their initial positions. These changes were introduced step-by-step until a borderline situation just short of complete acoustic decoupling was reached. Each step was examined using both LDV and cbCT and observed through the operation microscope. RESULTS: LDV was able to quantify the mechanic function of the ossicular chain after most of the manipulation steps by demonstrating the effect of any shifting of the prosthesis on the middle ear transfer function. However, in some situations, the middle ear transfer function was better with a visually more advanced failure of the prosthesis. In addition, cbCT showed most of the steps with excellent resolution and was able to delineate changes in soft tissue (e.g., cartilage covering). CONCLUSION: cbCT seems to be a promising imaging technique for middle ear problems. As cbCT and LDV exhibited slightly different advantages and disadvantages regarding the demonstration of borderline situations, the combination of both techniques allowed for a more precise evaluation of middle ear reconstructions. Knowledge of the specific characteristics of these methods and their possible combination might help otologists and otosurgeons to refine indications for revision surgery and improve their personal patient counseling.
Asunto(s)
Tomografía Computarizada de Haz Cónico , Prótesis Osicular , Reemplazo Osicular , Hueso Temporal/diagnóstico por imagen , Osículos del Oído/diagnóstico por imagen , Osículos del Oído/fisiología , Osículos del Oído/cirugía , Humanos , Falla de Prótesis , Membrana Timpánica/fisiologíaRESUMEN
OBJECTIVE: In order to help a surgeon make the best decision, a more objective method of measuring ossicular motion is required. METHODS: A laser Doppler vibrometer was mounted on a surgical microscope. To measure ossicular chain vibrations, eight patients with cochlear implants were investigated. To assess the motions of the ossicular chain, velocities at five points were measured with tonal stimuli of 1 and 3 kHz, which yielded reproducible results. The sequential amplitude change at each point was calculated with phase shifting from the tonal stimulus. Motion of the ossicular chain was visualized from the averaged results using the graphics application. RESULTS: The head of the malleus and the body of the incus showed synchronized movement as one unit. In contrast, the stapes (incudostapedial joint and posterior crus) moved synchronously in opposite phase to the malleus and incus. The amplitudes at 1 kHz were almost twice those at 3 kHz. CONCLUSIONS: Our results show that the malleus and incus unit and the stapes move with a phase difference.
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Osículos del Oído/fisiología , Humanos , Microscopía Confocal/métodosRESUMEN
Animals are frequently used for the development and testing of new hearing devices. Dimensions of the middle ear and cochlea differ significantly between humans and commonly used animals, such as rodents or cats. The sheep cochlea is anatomically more like the human cochlea in size and number of turns. This study investigated the middle-ear ossicular velocities and intracochlear sound pressure (ICSP) in sheep temporal bones, with the aim of characterizing the sheep as an experimental model for implantable hearing devices. Measurements were made on fresh sheep temporal bones. Velocity responses of the middle ear ossicles at the umbo, long process of the incus and stapes footplate were measured in the frequency range of 0.25-8 kHz using a laser Doppler vibrometer system. Results were normalized by the corresponding sound pressure level in the external ear canal (PEC). Sequentially, ICSPs at the scala vestibuli and tympani were then recorded with custom MEMS-based hydrophones, while presenting identical acoustic stimuli. The sheep middle ear transmitted most effectively around 4.8 kHz, with a maximum stapes velocity of 0.2 mm/s/Pa. At the same frequency, the ICSP measurements in the scala vestibuli and tympani showed the maximum gain relative to the PEC (24 dB and 5 dB, respectively). The greatest pressure difference across the cochlear partition occurred between 4 and 6 kHz. A comparison between the results of this study and human reference data showed middle-ear resonance and best cochlear sensitivity at higher frequencies in sheep. In summary, sheep can be an appropriate large animal model for research and development of implantable hearing devices.
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Conducción Ósea , Cóclea/fisiología , Osículos del Oído/fisiología , Sonido , Hueso Temporal/fisiología , Estimulación Acústica , Acústica , Animales , Cóclea/anatomía & histología , Osículos del Oído/anatomía & histología , Diseño de Equipo , Audífonos , Humanos , Modelos Animales , Movimiento (Física) , Presión , Oveja Doméstica , Especificidad de la Especie , Hueso Temporal/anatomía & histología , Factores de Tiempo , Vibración , Microtomografía por Rayos XRESUMEN
Objective To systematically review the anatomy of the ossicular chain. Data Sources Google Scholar, PubMed, and otologic textbooks. Review Methods A systematic literature search was performed on January 26, 2015. Search terms used to discover articles consisted of combinations of 2 keywords. One keyword from both groups was used: [ ossicular, ossicle, malleus, incus, stapes] and [ morphology, morphometric, anatomy, variation, physiology], yielding more than 50,000 hits. Articles were then screened by title and abstract if they did not contain information relevant to human ossicular chain anatomy. In addition to this search, references of selected articles were studied as well as suggested relevant articles from publication databases. Standard otologic textbooks were screened using the search criteria. Results Thirty-three sources were selected for use in this review. From these studies, data on the composition, physiology, morphology, and morphometrics were acquired. In addition, any correlations or lack of correlations between features of the ossicular chain and other features of the ossicular chain or patient were noted, with bilateral symmetry between ossicles being the only important correlation reported. Conclusion There was significant variation in all dimensions of each ossicle between individuals, given that degree of variation, custom fitting, or custom manufacturing of prostheses for each patient could optimize prosthesis fit. From published data, an accurate 3-dimensional model of the malleus, incus, and stapes can be created, which can then be further modified for each patient's individual anatomy.
Asunto(s)
Osículos del Oído/anatomía & histología , Prótesis Osicular , Osículos del Oído/fisiología , Humanos , Diseño de Prótesis , Planificación EstratégicaRESUMEN
The stapes is held in the oval window by the stapedial annular ligament (SAL), which restricts total peak-to-peak displacement of the stapes. Previous studies have suggested that for moderate (<130 dB SPL) sound levels intracochlear pressure (PIC), measured at the base of the cochlea far from the basilar membrane, increases directly proportionally with stapes displacement (DStap), thus a current model of impulse noise exposure (the Auditory Hazard Assessment Algorithm for Humans, or AHAAH) predicts that peak PIC will vary linearly with DStap up to some saturation point. However, no direct tests of DStap, or of the relationship with PIC during such motion, have been performed during acoustic stimulation of the human ear. In order to examine the relationship between DStap and PIC to very high level sounds, measurements of DStap and PIC were made in cadaveric human temporal bones. Specimens were prepared by mastoidectomy and extended facial recess to expose the ossicular chain. Measurements of PIC were made in scala vestibuli (PSV) and scala tympani (PST), along with the SPL in the external auditory canal (PEAC), concurrently with laser Doppler vibrometry (LDV) measurements of stapes velocity (VStap). Stimuli were moderate (â¼100 dB SPL) to very high level (up to â¼170 dB SPL), low frequency tones (20-2560 Hz). Both DStap and PSV increased proportionally with sound pressure level in the ear canal up to approximately â¼150 dB SPL, above which both DStap and PSV showed a distinct deviation from proportionality with PEAC. Both DStap and PSV approached saturation: DStap at a value exceeding 150 µm, which is substantially higher than has been reported for small mammals, while PSV showed substantial frequency dependence in the saturation point. The relationship between PSV and DStap remained constant, and cochlear input impedance did not vary across the levels tested, consistent with prior measurements at lower sound levels. These results suggest that PSV sound pressure holds constant relationship with DStap, described by the cochlear input impedance, at these, but perhaps not higher, stimulation levels. Additionally, these results indicate that the AHAAH model, which was developed using results from small animals, underestimates the sound pressure levels in the cochlea in response to high level sound stimulation, and must be revised.
Asunto(s)
Cóclea/fisiología , Audición/fisiología , Rampa Timpánica/fisiología , Escala Vestibular/fisiología , Estribo/fisiología , Estimulación Acústica , Acústica , Cadáver , Osículos del Oído/fisiología , Oído Medio/fisiología , Impedancia Eléctrica , Humanos , Rayos Láser , Prótesis Osicular , Presión , Modelos de Riesgos Proporcionales , Ventana Redonda/fisiología , Sonido , Estribo/anatomía & histología , Hueso Temporal/anatomía & histología , Hueso Temporal/fisiologíaRESUMEN
A fully implantable hearing aid is introduced which is a combined sensor-actuator-transducer designed for insertion into the incudostapedial joint gap (ISJ). The active elements each consist of a thin titanium membrane with an applied piezoelectric single crystal. The effectiveness of the operating principle is verified in a temporal bone study. We also take a closer look at the influence of an implantation-induced increase in middle ear stiffness on the transducer's output. An assembly of the transducer with 1 mm thickness is built and inserted into six temporal bones. At this thickness, the stiffness of the annular ligament is considerably increased, which leads to a loss in functional gain for the transducer. It is assumed that a thinner transducer would reduce this effect. In order to examine the performance for a prospective reduced pretension, we increased the gap size at the ISJ by 0.5 mm by removing the capitulum of the stapes in four temporal bones. The TM is stimulated with a broadband multisine sound signal in the audiological frequency range. The movement of the stapes footplate is measured with a laser Doppler vibrometer. The sensor signal is digitally processed and the amplified signal drives the actuator. The resulting feedback is minimized by an active noise control least mean square (LMS) algorithm which is implemented on a field programmable gate array. The dynamic range and the functional gain of the transducer in the temporal bones are determined. The results are compared to measurements from temporal bones without ISJ extension and to the results of Finite Elements Model (FE model) simulations. In the frequency range above 2 kHz a functional gain of 30 dB and more is achieved. This proposes the transducer as a potential treatment for high frequency hearing loss, e.g. for patients with noise-induced hearing loss. The transducer offers sufficient results for a comprehensive application. Adaptations in the transducer design or surgical approach are necessary to cope with ligament stiffening issues. These cause insufficient performance for low frequencies under 1 kHz.