RESUMEN
Infections caused by Rasamsonia argillacea complex have been reported in various clinical settings. Cystic fibrosis (CF) is one of the main underlying conditions. An observational cohort study of CF patients with Rasamsonia in respiratory samples was conducted. Eight isolates from 6 patients were identified as R. argillacea complex and tested for antifungal susceptibility. All isolates had high MICs to voriconazole and posaconazole and low MECs to echinocandins. Four patients experienced lung function decline in the year preceding first Rasamsonia isolation. This continued in the year following first isolation in 3 out of 4 cases. Antifungal therapy was initiated in 2 patients, to which only one exhibited a clinical response. Three out of 6 patients died within 3 years of isolating Rasamsonia. Genotyping suggests that similar genotypes of Rasamsonia can persist in CF airways. Consistent with other fungi in CF, the clinical impact of airway colonisation by Rasamsonia is variable. In certain patients, Rasamsonia may be able to drive clinical decline. In others, though a clear impact on lung function may be difficult to determine, the appearance of Rasamsonia acts as a marker of disease severity. In others it does not appear to have an obvious clinical impact on disease progression.
Asunto(s)
Antifúngicos/farmacología , Azoles/farmacología , Enfermedades Transmisibles Emergentes/microbiología , Fibrosis Quística/complicaciones , Farmacorresistencia Fúngica , Eurotiales/aislamiento & purificación , Enfermedades Pulmonares Fúngicas/microbiología , Adulto , Niño , Estudios de Cohortes , Equinocandinas/farmacología , Eurotiales/clasificación , Eurotiales/efectos de los fármacos , Eurotiales/genética , Femenino , Genotipo , Técnicas de Genotipaje , Humanos , Masculino , Pruebas de Sensibilidad Microbiana , Persona de Mediana Edad , Técnicas de Tipificación Micológica , Adulto JovenRESUMEN
The aim of this work was to document molecular epidemiology of Rasamsonia argillacea species complex isolates from cystic fibrosis (CF) patients. In this work, 116 isolates belonging to this species complex and collected from 26 CF patients and one patient with chronic granulomatous disease were characterized using PCR amplification assays of repetitive DNA sequences and electrophoretic separation of amplicons (rep-PCR). Data revealed a clustering consistent with molecular species identification. A single species was recovered from most patients. Rasamsonia aegroticola was the most common species, followed by R. argillacea sensu stricto and R. piperina, while R. eburnea was not identified. Of 29 genotypes, 7 were shared by distinct patients while 22 were patient specific. In each clinical sample, most isolates exhibited an identical genotype. Genotyping of isolates recovered from sequential samples from the same patient confirmed the capability of R. aegroticola and R. argillacea isolates to chronically colonize the airways. A unique genotype was recovered from two siblings during a 6-month period. In the other cases, a largely dominant genotype was detected. Present results which support the use of rep-PCR for both identification and genotyping for the R. argillacea species complex provide the first molecular evidence of chronic airway colonization by these fungi in CF patients.
Asunto(s)
Fibrosis Quística/complicaciones , Eurotiales/clasificación , Eurotiales/aislamiento & purificación , Micosis/diagnóstico , Micosis/epidemiología , Reacción en Cadena de la Polimerasa/métodos , Análisis por Conglomerados , Electroforesis , Eurotiales/genética , Genotipo , Humanos , Técnicas Microbiológicas/métodos , Epidemiología Molecular , Micosis/microbiología , Secuencias Repetitivas de Ácidos Nucleicos/genéticaRESUMEN
Penicillium species are some of the most common fungi observed worldwide and have an important economic impact as well as being occasional agents of human and animal mycoses. A total of 118 isolates thought to belong to the genus Penicillium based on morphological features were obtained from the Fungus Testing Laboratory at the University of Texas Health Science Center in San Antonio (United States). The isolates were studied phenotypically using standard growth conditions. Molecular identification was made using two genetic markers, the internal transcribed spacer (ITS) and a fragment of the ß-tubulin gene. In order to assess phylogenetic relationships, maximum likelihood and Bayesian inference assessments were used. Antifungal susceptibility testing was performed according to CLSI document M38-A2 for nine antifungal drugs. The isolates were identified within three genera, i.e., Penicillium, Talaromyces, and Rasamsonia The most frequent species in our study were Penicillium rubens, P. citrinum, and Talaromyces amestolkiae The potent in vitro activity of amphotericin B (AMB) and terbinafine (TRB) and of the echinocandins against Penicillium and Talaromyces species might offer a good therapeutic alternative for the treatment of infections caused by these fungi.
Asunto(s)
Antifúngicos/farmacología , Eurotiales/efectos de los fármacos , Eurotiales/aislamiento & purificación , Micosis/diagnóstico , Animales , Análisis por Conglomerados , ADN de Hongos/química , ADN de Hongos/genética , ADN Espaciador Ribosómico/química , ADN Espaciador Ribosómico/genética , Eurotiales/clasificación , Eurotiales/genética , Humanos , Pruebas de Sensibilidad Microbiana , Técnicas de Tipificación Micológica , Micosis/veterinaria , Filogenia , Análisis de Secuencia de ADN , Tubulina (Proteína)/genética , Estados UnidosRESUMEN
Many obligate symbiotic fungi are difficult to maintain in culture, and there is a growing need for alternative approaches to obtaining tissue and subsequent genomic assemblies from such species. In this study, the genome of Elaphomyces granulatus was sequenced from sporocarp tissue. The genome assembly remains on many contigs, but gene space is estimated to be mostly complete. Phylogenetic analyses revealed that the Elaphomyces lineage is most closely related to Talaromyces and Trichocomaceae s.s. The genome of E. granulatus is reduced in carbohydrate-active enzymes, despite a large expansion in genome size, both of which are consistent with what is seen in Tuber melanosporum, the other sequenced ectomycorrhizal ascomycete. A large number of transposable elements are predicted in the E. granulatus genome, especially Gypsy-like long terminal repeats, and there has also been an expansion in helicases. The metagenome is a complex community dominated by bacteria in Bradyrhizobiaceae, and there is evidence to suggest that the community may be reduced in functional capacity as estimated by KEGG pathways. Through the sequencing of sporocarp tissue, this study has provided insights into Elaphomyces phylogenetics, genomics, metagenomics and the evolution of the ectomycorrhizal association.
Asunto(s)
Bradyrhizobiaceae/genética , ADN de Hongos/genética , Eurotiales/genética , Cuerpos Fructíferos de los Hongos/genética , Genoma Fúngico/genética , Metagenoma , Secuencia de Bases , Bradyrhizobiaceae/clasificación , Elementos Transponibles de ADN/genética , Eurotiales/clasificación , Metagenómica , Microbiota/genética , Micorrizas/genética , Filogenia , Análisis de Secuencia de ADN , Talaromyces/genéticaRESUMEN
We describe two novel fungi isolated from soil samples collected in Northern Argentina and belonging to the family Aspergillaceae of the order Eurotiales: Leiothecium cristatum sp. nov. and Aspergillus posadasensis sp. nov. Leiothecium cristatum sp. nov., represented by the ex-type strain FMR 11998(T) (â=âCBS 134260(T)â=âNBRC 109843(T)), is distinguishable morphologically from the type species of the genus, Leiothecium ellipsoideum, by the presence of irregular reticulate ascospores with two prominent equatorial crests, and Aspergillus posadasensis sp. nov., represented by the ex-type strain FMR 12168(T) (â=âCBS 134259(T)â=âNBRC 109845(T)), is differentiated from Aspergillus acanthosporus, the nearest species phylogenetically, by its non-sclerotioid ascomata and a lack of an asexual stage on all culture media tested. The taxonomic proposals are supported by the analysis of the sequences of the internal transcribed spacer region, the D1-D2 domains of the 28S rRNA gene, the fragments of the RNA polymerase II largest subunit, and the putative chaperonin complex related to TCP-1, ß-tubulin and calmodulin genes.
Asunto(s)
Eurotiales/clasificación , Filogenia , Microbiología del Suelo , Argentina , ADN de Hongos/genética , ADN Espaciador Ribosómico/genética , Eurotiales/genética , Eurotiales/aislamiento & purificación , Genes Fúngicos , Datos de Secuencia Molecular , Técnicas de Tipificación Micológica , ARN Ribosómico 28S/genética , Análisis de Secuencia de ADNRESUMEN
Since 2010, colonizations/infections by Rasamsonia argillacea species complex, previously known as Geosmithia argillacea, have been regularly reported in literature. We reviewed all available cases focusing on pathogenesis and clinical relevance. The number of cases may be underestimated, as these fungi are frequently misidentified as Penicillium or Paecilomyces species. Major underlying conditions that predispose for infections by the R. argillacea species complex include cystic fibrosis (CF) and chronic granulomatous disease (CGD). While the pathogenic role of the colonization of CF lungs is still under debate, these molds are the causative agent of pneumonia and/or invasive infections in CGD patients. Given their thermotolerance and their resistance to various antifungals, especially the azole drugs, a special attention should be paid to the chronic colonization of the airways by these fungi in CF and CGD patients.
Asunto(s)
Eurotiales/clasificación , Eurotiales/fisiología , Micosis/diagnóstico , Micosis/terapia , Fibrosis Quística/complicaciones , Farmacorresistencia Fúngica , Eurotiales/aislamiento & purificación , Fungemia/microbiología , Enfermedad Granulomatosa Crónica/complicaciones , Humanos , Enfermedades Pulmonares Fúngicas/microbiología , Técnicas Microbiológicas/métodos , Micosis/microbiología , Micosis/patología , Factores de RiesgoRESUMEN
Geosmithia argillacea, an anamorph of Talaromyces eburneus, is a thermophilic filamentous fungus that has a phenotype similar to that of the Penicillium species, except for the creamy-white colonies and cylindrical conidia. Recently, a new genus called Rasamsonia has been proposed, which is to accommodate the Talaromyces and Geosmithia species. Here, we report the first Korean case of G. argillacea isolated from a patient with a fungal ball. The patient was a 44-yr-old Korean man with a history of pulmonary tuberculosis and aspergilloma. The newly developed fungal ball in his lung was removed and cultured to identify the fungus. The fungal colonies were white and slow-growing, and the filaments resembled those of Penicillium. Molecular identification was carried out by sequencing the internal transcribed spacer (ITS) region of the 28S rDNA and the ß-tubulin genes. A comparative sequence analysis using the GenBank (http://blast.ncbi.nlm.nih.gov/) database was performed with the basic local alignment search tool (BLAST) algorithm. The results revealed a 97-100% similarity with the G. argillacea ITS sequence. This case should increase awareness among physicians about the pathogenic potential of G. argillacea in humans and help them accurately identify this fungus, because it can be easily confused with Penicillium and Paecilomyces species owing to their similar phenotypic and microscopic characteristics. A molecular approach should be employed to enable accurate identification of G. argillacea.
Asunto(s)
Eurotiales/aislamiento & purificación , Tuberculosis/diagnóstico , Adulto , Bases de Datos Genéticas , Eurotiales/clasificación , Humanos , Pulmón/diagnóstico por imagen , Pulmón/microbiología , Masculino , Filogenia , ARN Ribosómico 28S/química , ARN Ribosómico 28S/genética , Análisis de Secuencia de ADN , Tomografía Computarizada por Rayos X , Tuberculosis/diagnóstico por imagen , Tuberculosis/microbiología , Tubulina (Proteína)/química , Tubulina (Proteína)/genéticaRESUMEN
Rasamsonia argillacea (formerly known as Geosmithia argillacea) is a fungus recently recognized as a pathogen of immunocompromised patients. Here we report the first case of Rasamsonia infection in an immunocompetent host, presenting as a pulmonary and aortic graft infection. Its morphological similarity to nonpathogenic Penicillium species delayed the diagnosis and initiation of appropriate treatment.
Asunto(s)
Aortitis/microbiología , Eurotiales , Huésped Inmunocomprometido , Enfermedades Pulmonares Fúngicas/microbiología , Aortitis/diagnóstico , Bronquiectasia/microbiología , Bronquiectasia/patología , Eurotiales/clasificación , Eurotiales/citología , Eurotiales/genética , Genes Bacterianos , Humanos , Enfermedades Pulmonares Fúngicas/diagnóstico , Masculino , Persona de Mediana Edad , Datos de Secuencia Molecular , Tomografía Computarizada por Rayos XRESUMEN
In recent years, Geosmithia argillacea has been increasingly reported in humans and animals and can be considered an emerging pathogen. The taxonomy of Geosmithia was recently studied, and Geosmithia argillacea and related species were transferred to the new genus Rasamsonia. The diversity among a set of Rasamsonia argillacea strains, including 28 clinical strains, was studied, and antifungal susceptibility profiles were generated. Data obtained from morphological studies and from phylogenetic analyses of internal transcribed spacer (ITS) and partial ß-tubulin and calmodulin sequences revealed the presence of four species in the Rasamsonia argillacea complex, two of which are newly described here: R. piperina sp. nov. and R. aegroticola sp. nov. In contrast to other related genera, all Rasamsonia species can be identified with ITS sequences. A retrospective identification was performed on recently reported clinical isolates from animal or human patients. Susceptibility tests showed that the antifungal susceptibility profiles of the four members of the R. argillacea complex are similar, and caspofungin showed significant activity in vitro, followed by amphotericin B and posaconazole. Voriconazole was the least active of the antifungals tested. The phenotypically similar species R. brevistipitata and R. cylindrospora had different antifungal susceptibility profiles, and this indicates that correct species identification is important to help guide appropriate antifungal therapy.
Asunto(s)
Antifúngicos/farmacología , Eurotiales/clasificación , Eurotiales/efectos de los fármacos , Micosis/microbiología , Filogenia , Animales , Análisis por Conglomerados , ADN de Hongos/química , ADN de Hongos/genética , ADN Ribosómico/química , ADN Ribosómico/genética , ADN Espaciador Ribosómico/química , ADN Espaciador Ribosómico/genética , Eurotiales/citología , Eurotiales/aislamiento & purificación , Humanos , Pruebas de Sensibilidad Microbiana , Datos de Secuencia Molecular , Análisis de Secuencia de ADN , Tubulina (Proteína)/genéticaRESUMEN
Geosmithia argillacea, an anamorph of Talaromyces eburneus, is a thermophilic filamentous fungus that has a phenotype similar to that of the Penicillium species, except for the creamy-white colonies and cylindrical conidia. Recently, a new genus called Rasamsonia has been proposed, which is to accommodate the Talaromyces and Geosmithia species. Here, we report the first Korean case of G. argillacea isolated from a patient with a fungal ball. The patient was a 44-yr-old Korean man with a history of pulmonary tuberculosis and aspergilloma. The newly developed fungal ball in his lung was removed and cultured to identify the fungus. The fungal colonies were white and slow-growing, and the filaments resembled those of Penicillium. Molecular identification was carried out by sequencing the internal transcribed spacer (ITS) region of the 28S rDNA and the beta-tubulin genes. A comparative sequence analysis using the GenBank (http://blast.ncbi.nlm.nih.gov/) database was performed with the basic local alignment search tool (BLAST) algorithm. The results revealed a 97-100% similarity with the G. argillacea ITS sequence. This case should increase awareness among physicians about the pathogenic potential of G. argillacea in humans and help them accurately identify this fungus, because it can be easily confused with Penicillium and Paecilomyces species owing to their similar phenotypic and microscopic characteristics. A molecular approach should be employed to enable accurate identification of G. argillacea.
Asunto(s)
Adulto , Humanos , Masculino , Bases de Datos Genéticas , Eurotiales/clasificación , Pulmón/microbiología , Filogenia , ARN Ribosómico 28S/química , Análisis de Secuencia de ADN , Tomografía Computarizada por Rayos X , Tuberculosis/diagnóstico , Tubulina (Proteína)/químicaRESUMEN
We report a comprehensive multi-year study of thermophilic fungi at the Sevilleta National Wildlife Refuge in central New Mexico. Recovery of thermophilic fungi from soils showed seasonal fluctuations, with greater abundance correlating with spring and summer precipitation peaks. In addition to grassland soils, we obtained and characterized isolates from grassland and riparian litter, herbivore dung and biological soil crusts. All strains belonged to either the Eurotiales or Sordariales (Chaetomiaceae). No particular substrate or microhabitat associations were detected. Molecular typing of strains revealed substantial phylogenetic diversity, eight ad hoc phylogroups across the two orders were identified and genetic diversity was present within each phylogroup. Growth tests over a range of temperatures showed substantial variation in maximum growth rates among strains and across phylogroups but consistency within phylogroups. Results demonstrated that 45-50 C represents the optimal temperature for growth of most isolates, with a dramatic decline at 60 C. Most strains grew at 60 C, albeit slowly, whereas none grew at 65 C, providing empirical confirmation that 60 C presents an evolutionary threshold for fungal growth. Our results support the hypothesis that fungal thermophily is an adaptation to transient seasonal and diurnal high temperatures, rather than simply an adaptation to specialized high-temperature environments. We note that the diversity observed among strains and the frequently confused taxonomy within these groups highlight the need for comprehensive biosystematic revision of thermophilic taxa in both orders.
Asunto(s)
Ecosistema , Eurotiales/aislamiento & purificación , Microbiología del Suelo , Sordariales/aislamiento & purificación , Adaptación Fisiológica , ADN Espaciador Ribosómico/genética , Eurotiales/clasificación , Eurotiales/genética , Eurotiales/crecimiento & desarrollo , Evolución Molecular , Genes de ARNr , Variación Genética , Calor , Técnicas de Tipificación Micológica , New Mexico , Filogenia , Estaciones del Año , Sordariales/clasificación , Sordariales/genética , Sordariales/crecimiento & desarrollo , Especificidad de la EspecieRESUMEN
Elaphomyces compleximurus sp. nov. and E. digitatus sp. nov. are described from the Pakaraima Mountains of Guyana. Macromorphological, micromorphological, habitat and DNA sequence data are provided for each new species. This is the first report of Elaphomyces ascomata associated with ectomycorrhizal members of the Fabaceae and also for the genus from the lowland South American tropics.
Asunto(s)
Eurotiales/clasificación , Micorrizas/clasificación , ADN de Hongos/genética , ADN Ribosómico/genética , Ecosistema , Eurotiales/genética , Eurotiales/ultraestructura , Guyana , Micorrizas/genética , Micorrizas/ultraestructura , Análisis de Secuencia de ADN/métodos , Clima TropicalRESUMEN
The phylogenetic relationship among Geosmithia argillacea, Talaromyces emersonii, Talaromyces byssochlamydoides and other members of the Trichocomaceae was studied using partial RPB2 (RNA polymerase II gene, encoding the second largest protein subunit), Tsr1 (putative ribosome biogenesis protein) and Cct8 (putative chaperonin complex component TCP-1) gene sequences. The results showed that these species form a distinct clade within the Trichocomaceae and Trichocoma paradoxa is phylogenetically most closely related. Based on phenotypic and physiological characters and molecular data, we propose Rasamsonia gen. nov. to accommodate these species. This new genus is distinct from other genera of the Trichocomaceae in being thermotolerant or thermophilic and having conidiophores with distinctly rough walled stipes, olive-brown conidia and ascomata, if present, with a scanty covering. Species within the genus Rasamsonia were distinguished using a combination of phenotypic characters, extrolite patterns, ITS and partial calmodulin and ß-tubulin sequences. Rasamsonia brevistipitata sp. nov. is described and five new combinations are proposed.
Asunto(s)
Microbiología Ambiental , Eurotiales/clasificación , Eurotiales/aislamiento & purificación , Micosis/microbiología , Plantas/microbiología , ADN de Hongos/genética , ADN Ribosómico/genética , Eurotiales/genética , Eurotiales/fisiología , Proteínas Fúngicas/genética , Calor , Humanos , Datos de Secuencia Molecular , Filogenia , Tubulina (Proteína)/genéticaRESUMEN
A bone marrow infection caused by Phialosimplex caninus was diagnosed in a seven-year-old female spayed Cocker Spaniel that was receiving prednisone for autoimmune hemolytic anemia. Histopathologic examination of a bone marrow core biopsy revealed clusters of oval to round yeast-like cells of varying shape and size and occasional irregular hyphae. Culture of a bone marrow aspirate sample yielded a mould initially suggestive of Paecilomyces inflatus or Sagenomella species but later determined to be P. caninus. The dog was treated with itraconazole and amphotericin B, and prednisone was continued at the lowest dose needed to control the hemolytic anemia. The patient died after 18 months of treatment. This is the first detailed clinical report of infection caused by P. caninus, a newly described fungus associated with disseminated disease in dogs.
Asunto(s)
Eurotiales/aislamiento & purificación , Huésped Inmunocomprometido , Mielitis/microbiología , Anfotericina B/uso terapéutico , Animales , Antifúngicos/uso terapéutico , Perros , Eurotiales/clasificación , Resultado Fatal , Femenino , Itraconazol/uso terapéutico , Mielitis/diagnóstico , Mielitis/tratamiento farmacológicoRESUMEN
The filamentous ascomycete Xeromyces bisporus is an extreme xerophile able to grow down to a water activity of 0.62. We have inferred the phylogenetic position of Xeromyces in relation to other xerophilic and xerotolerant fungi in the order Eurotiales. Using nrDNA and betatubulin sequences, we show that it is more closely related to the xerophilic foodborne species of the genus Chrysosporium, than to the genus Monascus. The taxonomy of X. bisporus and Monascus is discussed. Based on physiological, morphological, and phylogenetic distinctiveness, we suggest that Xeromyces should be retained as a separate genus.
Asunto(s)
Eurotiales/clasificación , Eurotiales/genética , Variación Genética , Filogenia , Agua/metabolismo , Eurotiales/metabolismo , Proteínas Fúngicas/genética , Datos de Secuencia MolecularRESUMEN
BACKGROUND: Chronic granulomatous disease (CGD) is an inherited disorder of the nicotinamide adenine dinucleotide phosphate oxidase that leads to defective production of microbicidal superoxide and other oxidative radicals, resulting in increased susceptibility to invasive infections, especially those due to fungi. METHODS: Geosmithia argillacea was identified from cultured isolates by genomic sequencing of the internal transcribed spacer region. Isolates previously identified as Paecilomyces variotii, a filamentous fungus closely resembling G. argillacea, were also examined. RESULTS: We identified G. argillacea as the cause of invasive mycosis in 7 CGD patients. In 5 cases, the fungus had been previously identified morphologically as P. variotii. All patients had pulmonary lesions; 1 had disseminated lesions following inhalational pneumonia. Infections involved the chest wall and contiguous ribs in 2 patients and disseminated to the brain in 1 patient. Four patients with pneumonia underwent surgical intervention. All patients responded poorly to medical treatment, and 3 died. CONCLUSIONS: We report the first cases of invasive mycosis caused by G. argillacea in CGD patients. G. argillacea infections in CGD are often refractory and severe with a high fatality rate. Surgical intervention has been effective in some cases. G. argillacea is a previously underappreciated and frequently misidentified pathogen in CGD that should be excluded when P. variotii is identified morphologically.
Asunto(s)
Enfermedades Transmisibles Emergentes/epidemiología , Enfermedades Transmisibles Emergentes/microbiología , Eurotiales/aislamiento & purificación , Enfermedad Granulomatosa Crónica/complicaciones , Micosis/epidemiología , Micosis/microbiología , Adolescente , Adulto , Niño , ADN de Hongos/química , ADN de Hongos/genética , ADN Espaciador Ribosómico/química , ADN Espaciador Ribosómico/genética , Eurotiales/clasificación , Eurotiales/genética , Femenino , Humanos , Masculino , Datos de Secuencia Molecular , Análisis de Secuencia de ADNRESUMEN
We report two cases of invasive infections due to Geosmithia argillacea, an emerging mold, in patients with chronic granulomatous disease receiving prolonged azole antifungal prophylaxis. One patient died despite receiving a combination of four antifungals, and the other developed cerebral and medullary lesions under a combination of caspofungin, posaconazole, terbinafine, and gamma interferon.
Asunto(s)
Antifúngicos/administración & dosificación , Azoles/administración & dosificación , Quimioprevención/métodos , Eurotiales/aislamiento & purificación , Enfermedad Granulomatosa Crónica/tratamiento farmacológico , Micosis/diagnóstico , Absceso/microbiología , Adolescente , Adulto , Caspofungina , ADN de Hongos/química , ADN de Hongos/genética , ADN Espaciador Ribosómico/química , ADN Espaciador Ribosómico/genética , Equinocandinas/administración & dosificación , Eurotiales/clasificación , Resultado Fatal , Femenino , Enfermedad Granulomatosa Crónica/complicaciones , Histocitoquímica , Humanos , Interferón gamma/administración & dosificación , Lipopéptidos , Masculino , Microscopía , Datos de Secuencia Molecular , Micosis/microbiología , Naftalenos/administración & dosificación , Análisis de Secuencia de ADN , Terbinafina , Triazoles/administración & dosificaciónRESUMEN
Poorly sporulating Aspergillus isolates from patients with cystic fibrosis (CF) are generally identified in routine procedures as Aspergillus spp. In this study, we identified and characterized 11 isolates belonging to two unusual Aspergillus species of the section Fumigati (A. lentulus and Neosartorya pseudofischeri) recovered from four different patients. Aspergillus lentulus was found occasionally during a 10-year follow-up study of one CF patient colonized by A. fumigatus. Neosartorya pseudofischeri was isolated from three patients followed in different European hospitals. This species was recovered from two sputum samples of one patient, and from four successive samples of the two other patients, suggesting that it may be responsible for chronic colonization. Both species were isolated together with A. fumigatus. Isolates from both species did not grow at 50°C, and DNA sequence analysis, together with further morphological observations permitted identification at the species level. Growth at different temperatures and antifungal susceptibility were also investigated. All the isolates of N. pseudofischeri exhibited a very low susceptibility to voriconazole (VRZ) whereas a very low susceptibility to VRZ and amphotericin B was seen with the A. lentulus isolates.
Asunto(s)
Aspergillus/clasificación , Aspergillus/aislamiento & purificación , Fibrosis Quística/microbiología , Eurotiales/clasificación , Eurotiales/aislamiento & purificación , Enfermedades Pulmonares Fúngicas/microbiología , Adolescente , Adulto , Antifúngicos/farmacología , Aspergillus/efectos de los fármacos , Aspergillus/genética , Medios de Cultivo , Eurotiales/efectos de los fármacos , Eurotiales/genética , Humanos , Masculino , Pruebas de Sensibilidad Microbiana , Técnicas de Tipificación Micológica , Aspergilosis Pulmonar/microbiología , Análisis de Secuencia de ADN , Especificidad de la Especie , Esputo/microbiologíaRESUMEN
AIMS: For identification of members of the fungal order Eurotiales, an 18S rRNA gene-based oligonucleotide microarray was developed and optimized. METHODS AND RESULTS: Eurotiales-specific probes covering most members of the Eurotiales as well as species-specific probes were designed and evaluated with three pure cultures (two fungi from the Eurotiales and one fungus from the Hypocreales). Nearly complete 18S rRNA genes of each reference culture were amplified and fluorescently labelled by random priming. CONCLUSIONS: Positive and negative hybridization results confirmed that the Eurotiales probes tested in this study could correctly identify members of the Eurotiales. The species-specific probes were also capable of species-level detection. SIGNIFICANCE AND IMPACT OF THE STUDY: These findings demonstrate the potential applications of a phylogenetic oligonucleotide microarray approach to characterizing fungal species and populations in environmental and other samples.
Asunto(s)
Eurotiales/genética , Análisis de Secuencia por Matrices de Oligonucleótidos/métodos , ARN Ribosómico 18S/genética , Eurotiales/clasificación , ARN de Hongos/genética , Especificidad de la EspecieRESUMEN
Aspergillus flavus is the major producer of carcinogenic aflatoxins in crops worldwide and is also an important opportunistic human pathogen in aspergillosis. The sexual state of this heterothallic fungus is described from crosses between strains of the opposite mating type. Sexual reproduction occurred between sexually compatible strains belonging to different vegetative compatibility groups. Multiple, indehiscent ascocarps containing asci and ascospores formed within the pseudoparenchymatous matrix of stromata, which places the fungus in genus Petromyces. The teleomorph of P. flavus could not be distinguished from that of P. parasiticus (anamorph = A. parasiticus), another aflatoxin-producing species, based on morphology of the sexual structures. The two species can be separated by anamorph morphology, mycotoxin profile and molecular characters.