RESUMEN
To improve the crop yield and quality, the cytosolic fructose-1,6-bisphosphatase (cFBPase) from mung bean (Vigna radiata), a rate-limiting enzyme in gluconeogenesis, was cloned, purified, and structurally characterised. To function it required Mg2+ and Mn2+ at 0.01-10 mM. The Michaelis-Menton constant and adenosine monophosphate (AMP) inhibitory constant (Ki) were 7.96 and 111.09 µM, respectively. The functional site residues of AMP binding (Arg30, Asp32, and Phe33) and the active site residues (Asn218 and Met251) were tested via site-directed mutagenesis and molecular docking. Asn218 and Met251 were replaced by Tyr and Leu, respectively. The M251L mutant showed enhanced substrate affinity and activity, resulting from decreased binding energy (-2.58 kcal·mol-1) and molecular distance (4.2 Å). AMP binding site mutations changed the enzyme activities, indicating a connection between the binding and active sites. Furthermore, Ki and docking analysis revealed that Asp32 plays a key role in maintaining the AMP binding conformation.
Asunto(s)
Citosol/enzimología , Fructosa-Bifosfatasa/genética , Fructosa-Bifosfatasa/aislamiento & purificación , Vigna/enzimología , Vigna/genética , Adenosina Monofosfato/metabolismo , Animales , Sitios de Unión , Clonación Molecular , Fructosa-Bifosfatasa/química , Fructosa-Bifosfatasa/metabolismo , Cinética , Simulación del Acoplamiento Molecular , Mutagénesis Sitio-Dirigida , Vigna/citologíaRESUMEN
The crystal structure of the class II fructose-1,6-bisphosphatase (FBPaseII) from the important pathogen Francisella tularensis is presented at 2.4â Å resolution. Its structural and functional relationships to the closely related phosphatases from Mycobacterium tuberculosis (MtFBPaseII) and Escherichia coli (EcFBPaseII) and to the dual phosphatase from Synechocystis strain 6803 are discussed. FBPaseII from F. tularensis (FtFBPaseII) was crystallized in a monoclinic crystal form (space group P21, unit-cell parameters a = 76.30, b = 100.17, c = 92.02â Å, ß = 90.003°) with four chains in the asymmetric unit. Chain A had two coordinated Mg2+ ions in its active center, which is distinct from previous findings, and is presumably deactivated by their presence. The structure revealed an approximate 222 (D2) symmetry homotetramer analogous to that previously described for MtFBPaseII, which is formed by a crystallographic dyad and which differs from the exact tetramer found in EcFBPaseII at a 222 symmetry site in the crystal. Instead, the approximate homotetramer is very similar to that found in the dual phosphatase from Synechocystis, even though no allosteric effector was found in FtFBPase. The amino-acid sequence and folding of the active site of FtFBPaseII result in structural characteristics that are more similar to those of the previously published EcFBPaseII than to those of MtFBPaseII. The kinetic parameters of native FtFBPaseII were found to be in agreement with published studies. Kinetic analyses of the Thr89Ser and Thr89Ala mutations in the active site of the enzyme are consistent with the previously proposed mechanism for other class II bisphosphatases. The Thr89Ala variant enzyme was inactive but the Thr89Ser variant was partially active, with an approximately fourfold lower Km and Vmax than the native enzyme. The structural and functional insights derived from the structure of FtFBPaseII will provide valuable information for the design of specific inhibitors.
Asunto(s)
Francisella tularensis/enzimología , Fructosa-Bifosfatasa/química , Fructosa-Bifosfatasa/metabolismo , Proteínas Bacterianas/química , Proteínas Bacterianas/genética , Proteínas Bacterianas/aislamiento & purificación , Proteínas Bacterianas/metabolismo , Dominio Catalítico , Cristalografía por Rayos X , Escherichia coli/enzimología , Fructosa-Bifosfatasa/genética , Fructosa-Bifosfatasa/aislamiento & purificación , Modelos Moleculares , Mycobacterium tuberculosis/enzimología , Conformación Proteica , Estructura Cuaternaria de Proteína , Synechocystis/enzimologíaRESUMEN
Serum-soluble folate binding protein (FBP) is an important tumor marker, and the development of a simple biosensing method is highly needed. In this work, a photoelectrochemical (PEC) biosensor for the detection of FBP was proposed based on the construction of an antifouling interface and the unique ligand-protein recognition. The PEC sensing platform was prepared by the biomimetic polydopamine (PDA) coating on TiO2 nanotubes arrays (NTAs). A significant PEC enhancement effect was obtained due to the macroporous structures. Excellent antifouling performance was achieved by conjugation of amino-group-terminated 8-arm poly(ethylene glycol) (PEG). The incorporation of folic acid (FA) retains the antifouling property and shows recognition abilities toward FBP. The fabricated PEC biosensor shows good analytical performance. The combination of ligand-protein recognition and a PEC antifouling interface provides a good consideration for the development of FBP biosensors.
Asunto(s)
Biomarcadores de Tumor/aislamiento & purificación , Técnicas Biosensibles , Neoplasias/sangre , Biomarcadores de Tumor/sangre , Ácido Fólico/química , Ácido Fólico/farmacología , Fructosa-Bifosfatasa/sangre , Fructosa-Bifosfatasa/aislamiento & purificación , Humanos , Indoles/química , Nanotubos/química , Neoplasias/patología , Procesos Fotoquímicos , Polietilenglicoles/química , Polímeros/química , Titanio/químicaRESUMEN
To enhance our understanding of the control of archaeal carbon central metabolism, a detailed analysis of the regulation mechanisms of both fructose1,6-bisphosphatase (FruBPase) and ADP-phosphofructokinase-1 (ADP-PFK1) was carried out in the methanogen Methanosarcina acetivorans. No correlations were found among the transcript levels of the MA_1152 and MA_3563 (frubpase type II and pfk1) genes, the FruBPase and ADP-PFK1 activities, and their protein contents. The kinetics of the recombinant FruBPase II and ADP-PFK1 were hyperbolic and showed simple mixed-type inhibition by AMP and ATP, respectively. Under physiological metabolite concentrations, the FruBPase II and ADP-PFK1 activities were strongly modulated by their inhibitors. To assess whether these enzymes were also regulated by a phosphorylation/dephosphorylation process, the recombinant enzymes and cytosolic-enriched fractions were incubated in the presence of commercial protein phosphatase or protein kinase. De-phosphorylation of ADP-PFK1 slightly decreased its activity (i.e. Vmax) and did not change its kinetic parameters and oligomeric state. Thus, the data indicated a predominant metabolic regulation of both FruBPase and ADP-PFK1 activities by adenine nucleotides and suggested high degrees of control on the respective pathway fluxes.
Asunto(s)
Proteínas Arqueales/metabolismo , Fructosa-Bifosfatasa/metabolismo , Methanosarcina/metabolismo , Fosfofructoquinasa-1/metabolismo , Adenosina Difosfato/metabolismo , Adenosina Monofosfato/metabolismo , Adenosina Trifosfato/metabolismo , Animales , Proteínas Arqueales/genética , Proteínas Arqueales/aislamiento & purificación , Pollos , Fructosa-Bifosfatasa/genética , Fructosa-Bifosfatasa/aislamiento & purificación , Fructosafosfatos/metabolismo , Genes Arqueales , Cinética , Methanosarcina/genética , Fosfofructoquinasa-1/genética , Fosfofructoquinasa-1/aislamiento & purificación , Fosforilación , Inhibidores de Proteínas Quinasas/metabolismo , Procesamiento Proteico-PostraduccionalRESUMEN
Cell-free biosystems comprised of synthetic enzymatic pathways would be a promising biomanufacturing platform due to several advantages, such as high product yield, fast reaction rate, easy control and access, and so on. However, it was essential to produce (purified) enzymes at low costs and stabilize them for a long time so to decrease biocatalyst costs. We studied the stability of the four recombinant enzyme mixtures, all of which originated from thermophilic microorganisms: triosephosphate isomerase (TIM) from Thermus thermophiles, fructose bisphosphate aldolase (ALD) from Thermotoga maritima, fructose bisphosphatase (FBP) from T. maritima, and phosphoglucose isomerase (PGI) from Clostridium thermocellum. It was found that TIM and ALD were very stable at evaluated temperature so that they were purified by heat precipitation followed by gradient ammonia sulfate precipitation. In contrast, PGI was not stable enough for heat treatment. In addition, the stability of a low concentration PGI was enhanced by more than 25 times in the presence of 20 mg/L bovine serum albumin or the other three enzymes. At a practical enzyme loading of 1000 U/L for each enzyme, the half-life time of free PGI was prolong to 433 h in the presence of the other three enzymes, resulting in a great increase in the total turn-over number of PGI to 6.2×10(9) mole of product per mole of enzyme. This study clearly suggested that the presence of other proteins had a strong synergetic effect on the stabilization of the thermolabile enzyme PGI due to in vitro macromolecular crowding effect. Also, this result could be used to explain why not all enzymes isolated from thermophilic microorganisms are stable in vitro because of a lack of the macromolecular crowding environment.
Asunto(s)
Proteínas Bacterianas/aislamiento & purificación , Fructosa-Bifosfatasa/aislamiento & purificación , Fructosa-Bifosfato Aldolasa/aislamiento & purificación , Glucosa-6-Fosfato Isomerasa/aislamiento & purificación , Triosa-Fosfato Isomerasa/aislamiento & purificación , Proteínas Bacterianas/química , Biocatálisis , Clostridium thermocellum/química , Clostridium thermocellum/enzimología , Pruebas de Enzimas , Estabilidad de Enzimas , Fructosa-Bifosfatasa/química , Fructosa-Bifosfato Aldolasa/química , Glucosa-6-Fosfato Isomerasa/química , Semivida , Cinética , Albúmina Sérica Bovina/química , Temperatura , Thermotoga maritima/química , Thermotoga maritima/enzimología , Thermus thermophilus/química , Thermus thermophilus/enzimología , Triosa-Fosfato Isomerasa/químicaRESUMEN
The glpX gene (Rv1099c) of Mycobacterium tuberculosis (Mtb) encodes Fructose 1,6-bisphosphatase II (FBPase II; EC 3.1.3.11); a key gluconeogenic enzyme. Mtb possesses glpX homologue as the major known FBPase. This study explored the expression, purification and enzymatic characterization of functionally active FBPase II from Mtb. The glpX gene was cloned, expressed and purified using a two step purification strategy including affinity and size exclusion chromatography. The specific activity of Mtb FBPase II is 1.3 U/mg. The enzyme is oligomeric, followed Michaelis-Menten kinetics with an apparent km = 44 µM. Enzyme activity is dependent on bivalent metal ions and is inhibited by lithium and inorganic phosphate. The pH optimum and thermostability of the enzyme have been determined. The robust expression, purification and assay protocols ensure sufficient production of this protein for structural biology and screening of inhibitors against this enzyme.
Asunto(s)
Fructosa-Bifosfatasa/genética , Fructosa-Bifosfatasa/aislamiento & purificación , Fructosa-Bifosfatasa/metabolismo , Mycobacterium tuberculosis/enzimología , Calcio/metabolismo , Calcio/farmacología , Cationes/metabolismo , Cromatografía de Afinidad , Cromatografía en Gel , Clonación Molecular , Inhibidores Enzimáticos/farmacología , Estabilidad de Enzimas , Escherichia coli/genética , Fructosa-Bifosfatasa/antagonistas & inhibidores , Regulación Enzimológica de la Expresión Génica , Concentración de Iones de Hidrógeno , Litio , Magnesio/metabolismo , Magnesio/farmacología , Manganeso/metabolismo , Manganeso/farmacología , Metales/metabolismo , Peso Molecular , Mycobacterium tuberculosis/genética , Proteínas Recombinantes/genética , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismoRESUMEN
Fructose-1,6-bisphosphatase is one of the key enzymes of the gluconeogenic pathway. It catalyses the hydrolysis of fructose-1,6-bisphosphate to fructose-6-phosphate and inorganic phosphate. Fructose-1,6-bisphosphatase from the extreme thermophilic bacterium Thermus thermophilus has been purified by crystallisation approach. The final well-shaped crystals have been obtained using vapour diffusion sitting-drops in the presence of PEG 400 as the precipitating agent. The initially obtained native twinned crystals diffracted up to 1.2Å resolution. Untwinned crystals used for data collection, however, were grown in the presence of thiomersal. They diffract to 1.8 Å resolution and belong to the space groups I422 with cell dimensions (i) a=b=108.8Å, c=336.3Å showing two molecules in the asymmetric unit, and (ii) a=b=113.7Å, c=151.0Å with one molecule in the asymmetric unit. The crystal structure has been solved by single anomalous dispersion using a 1.9Å resolution. For further biochemical and biophysical investigations recombinant fructose-1,6-bisphosphatase has been produced in Escherichia coli. Both native (dissolved crystals) and recombinant material have been characterised by SDS-PAGE, N-terminal sequencing and MALDI-MS.
Asunto(s)
Fructosa-Bifosfatasa/química , Fructosa-Bifosfatasa/aislamiento & purificación , Thermus thermophilus/enzimología , Secuencia de Aminoácidos , Cristalografía por Rayos X , Fructosa-Bifosfatasa/genética , Fructosa-Bifosfatasa/metabolismo , Datos de Secuencia Molecular , Proteínas Recombinantes/metabolismo , Alineación de Secuencia , Thermus thermophilus/metabolismoRESUMEN
To understand the physiological functions of thermostable fructose-1,6-bisphosphatase (TNA1-Fbp) from Thermococcus onnurineus NA1, its recombinant enzyme was overexpressed in Escherichia coli, purified, and the enzymatic properties were characterized. The enzyme showed maximal activity for fructose-1,6-bisphosphate at 95°C and pH 8.0 with a half-life (t (1/2)) of about 8 h. TNA1-Fbp had broad substrate specificities for fructose-1,6-bisphosphate and its analogues including fructose-1-phosphate, glucose-1-phosphate, and phosphoenolpyruvate. In addition, its enzyme activity was increased five-fold by addition of 1 mM Mg(2+), while Li(+) did not enhance enzymatic activity. TNA1-Fbp activity was inhibited by ATP, ADP, and phosphoenolpyruvate, but AMP up to 100 mM did not have any effect. TNA1-Fbp is currently defined as a class V fructose-1,6-bisphosphatase (FBPase) because it is very similar to FBPase of Thermococcus kodakaraensis KOD1 based on sequence homology. However, this enzyme shows a different range of substrate specificities. These results suggest that TNA1-Fbp can establish new criterion for class V FBPases.
Asunto(s)
Fructosa-Bifosfatasa/metabolismo , Thermococcus/enzimología , Adenosina Difosfato/metabolismo , Adenosina Trifosfato/metabolismo , Clonación Molecular , Activadores de Enzimas/metabolismo , Inhibidores Enzimáticos/metabolismo , Escherichia coli/genética , Fructosa-Bifosfatasa/química , Fructosa-Bifosfatasa/genética , Fructosa-Bifosfatasa/aislamiento & purificación , Fructosafosfatos/metabolismo , Expresión Génica , Glucofosfatos/metabolismo , Semivida , Calor , Concentración de Iones de Hidrógeno , Magnesio/metabolismo , Fosfoenolpiruvato/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Especificidad por SustratoRESUMEN
A mid-infrared enzymatic assay for label-free monitoring of the enzymatic reaction of fructose-1,6-bisphosphatase with fructose 1,6-bisphosphate has been proposed. The whole procedure was done in an automated way operating in the stopped flow mode by incorporating a temperature-controlled flow cell in a sequential injection manifold. Fourier transform infrared difference spectra were evaluated for kinetic parameters, like the Michaelis-Menten constant (K(M)) of the enzyme and Vmax of the reaction. The obtained K(M) of the reaction was 14 +/- 3 g L(-1) (41 microM). Furthermore, inhibition by adenosine 5'-monophosphate (AMP) was evaluated, and the K(M)(App) value was determined to be 12 +/- 2 g L(-1) (35 microM) for 7.5 and 15 microM AMP, respectively, with Vmax decreasing from 0.1 +/- 0.03 to 0.05 +/- 0.01 g L(-1) min(-1). Therefore, AMP exerted a non-competitive inhibition.
Asunto(s)
Adenosina Monofosfato/metabolismo , Análisis de Inyección de Flujo/instrumentación , Fructosa-Bifosfatasa/antagonistas & inhibidores , Fructosa-Bifosfatasa/metabolismo , Espectroscopía Infrarroja por Transformada de Fourier/métodos , Diseño de Equipo , Fructosa-Bifosfatasa/aislamiento & purificación , Fructosadifosfatos/metabolismo , Humanos , Cinética , Hígado/enzimología , Espectroscopía Infrarroja por Transformada de Fourier/instrumentaciónRESUMEN
It was shown for the first time that extracellular FBPase of B. subtilis 668 like the preparation obtained from culture liquid of B. subtilis B 7025 displays citotoxicity activity in respect of tumor cells of sarcoma 37 in vitro. It is shown that the preparations remove TA antigens from the surface of the tumor cell. It is supposed that the mechanisms of citotoxic effect of extracellular FBFase of B. subtilis 668 and preparation from the culture liquid of B. subtilis B 7025 in vitro on cells of sarcoma 37 is probably realized through the apoptosis.
Asunto(s)
Antineoplásicos/farmacología , Apoptosis/efectos de los fármacos , Bacillus subtilis/enzimología , Medios de Cultivo/química , Líquido Extracelular/enzimología , Fructosa-Bifosfatasa/farmacología , Animales , Antineoplásicos/aislamiento & purificación , Línea Celular Tumoral , Fructosa-Bifosfatasa/aislamiento & purificación , Sarcoma 37/patologíaRESUMEN
The p53 tumor-suppressor protein prevents cancer development through various mechanisms, including the induction of cell-cycle arrest, apoptosis, and the maintenance of genome stability. We have identified a p53-inducible gene named TIGAR (TP53-induced glycolysis and apoptosis regulator). TIGAR expression lowered fructose-2,6-bisphosphate levels in cells, resulting in an inhibition of glycolysis and an overall decrease in intracellular reactive oxygen species (ROS) levels. These functions of TIGAR correlated with an ability to protect cells from ROS-associated apoptosis, and consequently, knockdown of endogenous TIGAR expression sensitized cells to p53-induced death. Expression of TIGAR may therefore modulate the apoptotic response to p53, allowing survival in the face of mild or transient stress signals that may be reversed or repaired. The decrease of intracellular ROS levels in response to TIGAR may also play a role in the ability of p53 to protect from the accumulation of genomic damage.
Asunto(s)
Apoptosis/genética , Transformación Celular Neoplásica/metabolismo , Glucólisis/genética , Estrés Oxidativo/genética , Monoéster Fosfórico Hidrolasas/metabolismo , Proteínas/metabolismo , Proteína p53 Supresora de Tumor/metabolismo , Secuencia de Aminoácidos , Proteínas Reguladoras de la Apoptosis , Secuencia de Bases , Línea Celular Tumoral , Supervivencia Celular/genética , Transformación Celular Neoplásica/genética , Cromosomas Humanos Par 12/genética , Daño del ADN/fisiología , Reparación del ADN/fisiología , Regulación hacia Abajo/fisiología , Metabolismo Energético/genética , Fructosa-Bifosfatasa/genética , Fructosa-Bifosfatasa/aislamiento & purificación , Fructosa-Bifosfatasa/metabolismo , Fructosadifosfatos/metabolismo , Regulación de la Expresión Génica/fisiología , Inestabilidad Genómica/fisiología , Humanos , Péptidos y Proteínas de Señalización Intracelular , Datos de Secuencia Molecular , Monoéster Fosfórico Hidrolasas/genética , Monoéster Fosfórico Hidrolasas/aislamiento & purificación , Proteínas/genética , Proteínas/aislamiento & purificación , Especies Reactivas de Oxígeno/metabolismo , Transducción de Señal/fisiología , Proteína p53 Supresora de Tumor/genéticaRESUMEN
Fructose 1,6-bisphosphatase (FBPase) is known to form a supramolecular complex with alpha-actinin and aldolase on both sides of the Z-line in skeletal muscle cells. It has been proposed that association of aldolase with FBPase not only desensitizes muscle FBPase toward AMP inhibition but it also might enable the channeling of intermediates between the enzymes [Rakus et al. (2003) FEBS Lett. 547, 11-14]. In the present paper, we tested the possibility of fructose 1,6-bisphosphate (F1,6-P(2)) channeling between aldolase and FBPase using the approach in which an inactive form of FBPase competed with active FBPase for binding to aldolase and thus decreased the rate of aldolase-FBPase reaction. The results showed that F1,6-P(2) is transferred directly from aldolase to FBPase without mixing with the bulk phase. Further evidence that F1,6-P(2) is channeled from aldolase to FBPase comes from the experiments investigating the inhibitory effect of a high concentration of magnesium ions on aldolase-FBPase activity. FBPase in a complex with aldolase, contrary to free muscle FBPase, was not inhibited by high Mg(2+) concentrations, which suggests that free F1,6-P(2) was not present in the assay mixture during the reaction. A real-time interaction analysis between aldolase and FBPase revealed a dual role of Mg(2+) in the regulation of the aldolase-FBPase complex stability. A physiological concentration of Mg(2+) increased the affinity of muscle FBPase to muscle aldolase, whereas higher concentrations of the cation decreased the concentration of the complex. We hypothesized that the presence of Mg(2+) stabilizes a positively charged cavity within FBPase and that it might enable an interaction with aldolase. Because magnesium decreased the binding constant (K(a)) between aldolase and FBPase in a manner similar to the decrease of K(a) caused by monovalent cations, it is postulated that electrostatic attraction might be a driving force for the complex formation. It is presumed that the biological relevance of F1,6-P(2) channeling between aldolase and FBPase is protection of this glyconeogenic, as well as glycolytic, intermediate against degradation by cytosolic aldolase, which is one of the most abundant enzyme of glycolysis.
Asunto(s)
Fructosa-Bifosfatasa/metabolismo , Fructosa-Bifosfato Aldolasa/metabolismo , Músculo Esquelético/química , Músculo Esquelético/enzimología , Especificidad por Sustrato , Actinina/metabolismo , Adenosina Monofosfato/farmacología , Animales , Cationes Monovalentes/farmacología , Simulación por Computador , Relación Dosis-Respuesta a Droga , Inhibidores Enzimáticos/farmacología , Fructosa-Bifosfatasa/antagonistas & inhibidores , Fructosa-Bifosfatasa/aislamiento & purificación , Fructosa-Bifosfato Aldolasa/antagonistas & inhibidores , Fructosa-Bifosfato Aldolasa/aislamiento & purificación , Cinética , Magnesio/metabolismo , Magnesio/farmacología , Modelos Moleculares , Polietilenglicoles , Unión Proteica , Desnaturalización Proteica , Fosfato de Piridoxal/análogos & derivados , Fosfato de Piridoxal/farmacología , Conejos , Espectrometría de Fluorescencia , Electricidad Estática , Resonancia por Plasmón de Superficie , Temperatura , o-Ftalaldehído/farmacologíaRESUMEN
A preparation with fructose-1,6-bisphosphatase (FBPase) activity has been isolated as a result of the five-stage treatment of the culture liquid obtained after growing the agent of pale-green dwarfness of cereals on the medium CM IMB-72. After this enzyme treatment by means of hydrophobic chromatography on the column from Toyopearl HW-60 (the enzyme was obtained from the column with decrease of ammonium sulphate (AS) concentration in the eluating buffer to 0.8 M), the preparation deprivation of AS on the column with Sephadex G-10 and substrate-dependent chromatography on the column with CM-sepharose the extracellular 176-fold purified FBPase. Acholeplasma laidlawii var. granulum strain 118 was obtained--the main pathogenicity factor for the agent of cereals yellow.
Asunto(s)
Grano Comestible , Fructosa-Bifosfatasa/aislamiento & purificación , Phytoplasma/patogenicidad , Enfermedades de las Plantas , Cromatografía , Medios de Cultivo , Fructosa-Bifosfatasa/química , Fructosa-Bifosfatasa/genética , Interacciones Hidrofóbicas e HidrofílicasRESUMEN
Fructose-1,6-bisphosphatase (FBPase; EC 3.1.3.11) is strongly inhibited by AMP in vitro and, therefore, at physiological concentrations of substrate and AMP, FBPase should be completely inhibited. Desensitization of rabbit muscle FBPase against AMP inhibition was previously observed in the presence of rabbit muscle aldolase. In this study, we analysed the kinetics of an FBPase catalyzed reaction and interaction between chicken muscle FBPase and chicken muscle aldolase. The initial rate of FBPase reaction vs. substrate concentration shows a maximum activity at a concentration of 20 microM Fru-1,6P2 and then decreases. Assuming rapid equilibrium kinetics, the enzyme-catalyzed reaction was described by the substrate inhibition model, with Ks approximately 5 microM and Ksi approximately 39 microM and factor beta approximately 0.2, describing change in the rate constant (k) of product formation from the ES and ESSi complexes. Based on ultracentrifugation studies, aldolase and FBPase form a hetero-complex with approximately 1:1 stoichiometry with a dissociation constant (Kd) of 3.8 microM. The FBPase-aldolase interaction was confirmed via fluorescence investigation. The aldolase-FBPase interaction results in aldolase fluorescence quenching and its maximum emission spectrum shifting from 344 to 356 nm. The Kd of the FBPase-aldolase complex, determined on the basis of fluorescence changes, is 0.4 microM at 25 degrees C with almost 1:1 stoichiometry. This interaction increases the I(0.5) for the AMP inhibition of FBPase threefold, and slightly affects FBPase affinity to magnesium ions, increasing the Ka and Hill coefficient (n). No effect of aldolase on the FBPase pH optimum was observed. Thus, the decrease in FBPase sensitivity to AMP inhibition enables FBPase to function in vivo thanks to aldolase.
Asunto(s)
Fructosa-Bifosfatasa/metabolismo , Fructosa-Bifosfato Aldolasa/metabolismo , Músculo Esquelético/enzimología , Adenosina Monofosfato/metabolismo , Animales , Pollos , Fructosa-Bifosfatasa/antagonistas & inhibidores , Fructosa-Bifosfatasa/aislamiento & purificación , Fructosa-Bifosfato Aldolasa/antagonistas & inhibidores , Fructosa-Bifosfato Aldolasa/aislamiento & purificación , Cinética , Unión Proteica , Espectrometría de Fluorescencia , Especificidad por SustratoRESUMEN
Real-time interaction analysis, using the BIAcore biosensor, of rabbit muscle FBPase-aldolase complex revealed apparent binding constant [K(Aapp)] values of about 4.4x10(8) M(-1). The stability of the complex was down-regulated by the glycolytic intermediates dihydroxyacetone phosphate and fructose 6-phosphate, and by the regulator of glycolysis and glyconeogenesis--fructose 2,6-bisphosphate. FBPase in a complex with aldolase was entirely insensitive to inhibition by physiological concentrations of AMP (I(0.5) was 1.35 mM) and the cooperativity of the inhibition was not observed. The existence of an FBPase-aldolase complex that is insensitive to AMP inhibition explains the possibility of glycogen synthesis from carbohydrate precursors in vertebrates' myocytes.
Asunto(s)
Adenosina Monofosfato/farmacología , Fructosa-Bifosfatasa/antagonistas & inhibidores , Fructosa-Bifosfato Aldolasa/antagonistas & inhibidores , Músculo Esquelético/enzimología , Animales , Dihidroxiacetona Fosfato/farmacología , Inhibidores Enzimáticos/farmacología , Fructosa-Bifosfatasa/aislamiento & purificación , Fructosa-Bifosfatasa/metabolismo , Fructosa-Bifosfato Aldolasa/aislamiento & purificación , Fructosa-Bifosfato Aldolasa/metabolismo , Gluconeogénesis , Glucólisis , Cinética , ConejosRESUMEN
Phosphorylated fructose-1,6-bisphosphatase (FBPase) was isolated from rabbit muscle in an SDS/PAGE homogeneous form. Its dephosphorylation with alkaline phosphatase revealed 2.8 moles of inorganic phosphate per mole of FBPase. The phosphorylated FBPase (P-FBPase) differs from the dephosphorylated enzyme in terms of its kinetic properties like K(m) and k(cat), which are two times higher for the phosphorylated FBPase, and in the affinity for aldolase, which is three times lower for the dephosphorylated enzyme. Dephosphorylated FBPase can be a substrate for protein kinase A and the amount of phosphate incorporated per FBPase monomer can reach 2-3 molecules. Since interaction of muscle aldolase with muscle FBPase results in desensitisation of the latter toward AMP inhibition (Rakus & Dzugaj, 2000, Biochem. Biophys. Res. Commun. 275, 611-616), phosphorylation may be considered as a way of muscle FBPase activity regulation.
Asunto(s)
Fructosa-Bifosfatasa/metabolismo , Músculo Esquelético/enzimología , Animales , Proteínas Quinasas Dependientes de AMP Cíclico/metabolismo , Electroforesis en Gel de Poliacrilamida , Fructosa-Bifosfatasa/química , Fructosa-Bifosfatasa/aislamiento & purificación , Cinética , Modelos Moleculares , Fosfatos/metabolismo , Fosforilación , Conformación Proteica , Conejos , Especificidad por SustratoRESUMEN
Full-length cDNA encoding pea cytoplasmic fructose-1,6-bisphosphatase (cyFBPase) was cloned from a pea cDNA library. The cloned cDNA was introduced into the Escherichia coli expression vector pET-15b. The recombinant cyFBPase was expressed in E. coli BL21 (DE3) cells in a soluble form and purified to homogeneity by Ni(+)-NTA affinity chromatography. The identity of the recombinant cyFBPase was confirmed by SDS-PAGE and immunoblot analysis using a polyclonal anti-His tag antibody. The recombinant cyFBPase was active at neutral pH ranges (6.6-9.0) and thermostable as other cyFBPases. The activation energy (E(a)) and Arrhenius frequency factor were 17.4 kcal/mol and 2.6 x 10(12)/s, respectively. The K(M) and V(max) values of the recombinant enzyme were calculated as 10.47 microM and 109 micromol/min, respectively. In case of removal of histidine tag, the K(M) value was calculated as 5.03 microM. The recombinant enzyme was non-competitively and competitively inhibited by AMP and fructose-2,6-bisphosphate, respectively.
Asunto(s)
Citoplasma/enzimología , Fructosa-Bifosfatasa/química , Fructosa-Bifosfatasa/aislamiento & purificación , Pisum sativum/enzimología , Secuencia de Aminoácidos , Clonación Molecular , Estabilidad de Enzimas , Escherichia coli , Fructosa-Bifosfatasa/genética , Fructosa-Bifosfatasa/metabolismo , Expresión Génica , Concentración de Iones de Hidrógeno , Cinética , Datos de Secuencia Molecular , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Homología de Secuencia de Aminoácido , TemperaturaRESUMEN
The Pyrococcus furiosus fbpA gene was cloned and expressed in Escherichia coli, and the fructose-1,6-bisphosphatase produced was subsequently purified and characterized. The dimeric enzyme showed a preference for fructose-1,6-bisphosphate, with a K(m) of 0.32 mM and a V(max) of 12.2 U/mg. The P. furiosus fructose-1,6-bisphosphatase was strongly inhibited by Li(+) (50% inhibitory concentration, 1 mM). Based on the presence of conserved sequence motifs and the substrate specificity of the P. furiosus fructose-1,6-bisphosphatase, we propose that this enzyme belongs to a new family, class IV fructose-1,6-bisphosphatase.
Asunto(s)
Proteínas Arqueales/genética , Proteínas Arqueales/metabolismo , Fructosa-Bifosfatasa , Pyrococcus furiosus/enzimología , Secuencia de Aminoácidos , Clonación Molecular , Fructosa-Bifosfatasa/clasificación , Fructosa-Bifosfatasa/genética , Fructosa-Bifosfatasa/aislamiento & purificación , Fructosa-Bifosfatasa/metabolismo , Datos de Secuencia Molecular , Pyrococcus furiosus/genética , Análisis de Secuencia de ADN , Especificidad por Sustrato , TemperaturaRESUMEN
Previous kinetic characterization of Escherichia coli fructose 1,6-bisphosphatase (FBPase) was performed on enzyme with an estimated purity of only 50%. Contradictory kinetic properties of the partially purified E. coli FBPase have been reported in regard to AMP cooperativity and inactivation by fructose-2,6-bisphosphate. In this investigation, a new purification for E. coli FBPase has been devised yielding enzyme with purity levels as high as 98%. This highly purified E. coli FBPase was characterized and the data compared to that for the pig kidney enzyme. Also, a homology model was created based upon the known three-dimensional structure of the pig kidney enzyme. The kcat of the E. coli FBPase was 14.6 s(-1) as compared to 21 s(-1) for the pig kidney enzyme, while the K(m) of the E. coli enzyme was approximately 10-fold higher than that of the pig kidney enzyme. The concentration of Mg2+ required to bring E. coli FBPase to half maximal activity was estimated to be 0.62 mM Mg2+, which is twice that required for the pig kidney enzyme. Unlike the pig kidney enzyme, the Mg2+ activation of the E. coli FBPase is not cooperative. AMP inhibition of mammalian FBPases is cooperative with a Hill coefficient of 2; however, the E. coli FBPase displays no cooperativity. Although cooperativity is not observed, the E. coli and pig kidney enzymes show similar AMP affinity. The quaternary structure of the E. coli enzyme is tetrameric, although higher molecular mass aggregates were also observed. The homology model of the E. coli enzyme indicated slight variations in the ligand-binding pockets compared to the pig kidney enzyme. The homology model of the E. coli enzyme also identified significant changes in the interfaces between the subunits, indicating possible changes in the path of communication of the allosteric signal.
Asunto(s)
Escherichia coli/enzimología , Fructosa-Bifosfatasa/química , Regulación Alostérica , Secuencia de Aminoácidos , Cationes Bivalentes , Inhibidores Enzimáticos/farmacología , Escherichia coli/genética , Fructosa-Bifosfatasa/genética , Fructosa-Bifosfatasa/aislamiento & purificación , Cinética , Magnesio/química , Modelos Moleculares , Datos de Secuencia Molecular , Proteínas Recombinantes/química , Alineación de Secuencia , Transducción de SeñalRESUMEN
Hypertrehalosemic neuropeptides from the corpora cardiaca such as the decapeptide Bld HrTH bring about a profound switch in the metabolic activity of cockroach fat body during which production of the blood sugar trehalose is stimulated while the catabolism of carbohydrate (glycolysis) is inhibited. The mechanisms of the metabolic switch are not fully understood. Incubation of isolated fat body from the cockroach Blaptica dubia with 10(-8) M Bld HrTH, for 10-60 min, stimulated glycogen breakdown and increased the content of the substrates of both the glycolytic enzyme 6-phosphofructo-1-kinase (PFK, EC 2.7.1.11) and the gluconeogenic enzyme fructose-1,6-bisphosphatase (FBPase, EC 3.1.3.11) in the tissue. The glycolytic signal fructose 2,6-bisphosphate was markedly decreased in fat body on incubation with Bld HrTH. The content of ATP was slightly reduced, while the contents of ADP and AMP were increased after incubation with the hormone. Fructose 2,6-bisphosphate is a potent activator of PFK and a strong inhibitor of FBPase purified from fat body. The activity of PFK was decreased by about 90% when the hormone-dependent changes in effectors and substrates in fat body were simulated in vitro. FBPase, in contrast, was activated about 25-fold under these conditions, suggesting the hormone to stimulate gluconeogenesis in fat body. The data support the view that fructose 2,6-bisphosphate is a pivotal intracellular messenger in the hormone-induced metabolic switch from carbohydrate degradation to trehalose production in cockroach fat body.