RESUMEN
In this study, we investigated the trimerization mechanism and structure of heat shock factor 1 (HSF1) using western blotting, tryptophan (Trp) fluorescence spectroscopy, and molecular modeling. First, we examined the DNA-binding domains of human (Homo sapiens), goldfish (Carassius auratus), and walleye pollock (Gadus chalcogrammus) HSF1s by mutating key residues (36 and 103) that are thought to directly affect trimer formation. Human, goldfish, and walleye pollock HSF1s contain cysteine at residue 36 but cysteine (C), tyrosine (Y), and phenylalanine (F), respectively, at residue 103. The optimal trimerization temperatures for the wild-type HSF1s of each species were found to be 42, 37, and 20 °C, respectively. Interestingly, a mutation experiment revealed that trimerization occurred at 42 °C when residue 103 was cysteine, at 37 °C when it was tyrosine, and at 20 °C when it was phenylalanine, regardless of the species. In addition, it was confirmed that when residue 103 of the three species was mutated to alanine, trimerization did not occur. This suggests that in addition to trimerization via disulfide bond formation between the cysteine residues in human HSF1, trimerization can also occur via the formation of a different type of bond between cysteine and aromatic ring residues such as tyrosine and phenylalanine. We also confirmed that at least one cysteine is required for the trimerization of HSF1s, regardless of its position (residue 36 or 103). Additionally, it was shown that the trimer formation temperature is related to growth and survival in fish.
Asunto(s)
Aminoácidos Aromáticos , Cisteína , Factores de Transcripción del Choque Térmico , Animales , Humanos , Aminoácidos Aromáticos/metabolismo , Aminoácidos Aromáticos/química , Cisteína/química , Cisteína/metabolismo , Proteínas de Unión al ADN/química , Proteínas de Unión al ADN/metabolismo , Proteínas de Unión al ADN/genética , Carpa Dorada/metabolismo , Factores de Transcripción del Choque Térmico/metabolismo , Factores de Transcripción del Choque Térmico/química , Factores de Transcripción del Choque Térmico/genética , Modelos Moleculares , Dominios Proteicos , Multimerización de ProteínaRESUMEN
Cell division and cell cycle mechanism has been studied for 70 years. This research has revealed that the cell cycle is regulated by many factors, including cyclins and cyclin-dependent kinases (CDKs). Heat shock transcription factors (HSFs) have been noted as critical proteins for cell survival against various stresses; however, recent studies suggest that HSFs also have important roles in cell cycle regulation-independent cell-protective functions. During cell cycle progression, HSF1, and HSF2 bind to condensed chromatin to provide immediate precise gene expression after cell division. This review focuses on the function of these HSFs in cell cycle progression, cell cycle arrest, gene bookmarking, mitosis and meiosis.
Asunto(s)
Ciclo Celular , Factores de Transcripción del Choque Térmico/metabolismo , Animales , Puntos de Control del Ciclo Celular , Factores de Transcripción del Choque Térmico/química , Humanos , Meiosis , ProteolisisRESUMEN
Small heat shock proteins (sHSPs) function as molecular chaperones in multiple physiological processes and are active during thermal stress. sHSP expression is controlled by heat shock transcription factor (HSF); however, few studies have been conducted on HSF in agricultural pests. Liriomyza trifolii is an introduced insect pest of horticultural and vegetable crops in China. In this study, the master regulator, HSF1, was cloned and characterized from L. trifolii, and the expression levels of HSF1 and five sHSPs were studied during heat stress. HSF1 expression in L. trifolii generally decreased with rising temperatures, whereas expression of the five sHSPs showed an increasing trend that correlated with elevated temperatures. All five sHSPs and HSF1 showed an upward trend in expression with exposure to 40 â without a recovery period. When a recovery period was incorporated after thermal stress, the expression patterns of HSF1 and sHSPs in L. trifolii exposed to 40 °C was significantly lower than expression with no recovery period. To elucidate potential interactions between HSF1 and sHSPs, double-stranded RNA was synthesized to knock down HSF1 in L. trifolii by RNA interference. The knockdown of HSF1 by RNAi decreased the survival rate and expression of HSP19.5, HSP20.8, and HSP21.3 during high-temperature stress. This study expands our understanding of HSF1-regulated gene expression in L. trifolii exposed to heat stress.
Asunto(s)
Dípteros/genética , Dípteros/fisiología , Regulación de la Expresión Génica , Factores de Transcripción del Choque Térmico/metabolismo , Proteínas de Choque Térmico Pequeñas/genética , Respuesta al Choque Térmico/genética , Adaptación Fisiológica/genética , Envejecimiento/genética , Secuencia de Aminoácidos , Animales , Factores de Transcripción del Choque Térmico/química , Proteínas de Choque Térmico Pequeñas/metabolismo , Filogenia , Interferencia de ARNRESUMEN
Heat Shock Factor 1 (HSF1) is the master regulator of the heat shock response, a universal survival mechanism throughout eukaryotic species used to buffer potentially lethal proteotoxic conditions. HSF1's function in vivo is regulated by several factors, including post translational modifications and elevated temperatures, whereupon it forms trimers to bind with heat shock elements in DNA. Unsurprisingly, HSF1 is also extremely sensitive to elevated temperatures in vitro, which poses specific technical challenges when producing HSF1 using a recombinant expression system. Although there are several useful publications which outline steps taken for HSF1 expression and purification, studies that describe specific strategies and detailed protocols to overcome HSF1 trimerisation and degradation are currently lacking. Herein, we have reported our detailed experimental protocol for the expression and purification of monomeric human HSF1 (HsHSF1) as a major species. We also propose a refined method of inducing HsHSF1 activation in vitro, that we consider more accurately mimics HsHSF1 activation in vivo and is therefore more physiologically relevant.
Asunto(s)
Expresión Génica , Factores de Transcripción del Choque Térmico , Factores de Transcripción del Choque Térmico/biosíntesis , Factores de Transcripción del Choque Térmico/química , Factores de Transcripción del Choque Térmico/genética , Factores de Transcripción del Choque Térmico/aislamiento & purificación , Humanos , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/aislamiento & purificaciónRESUMEN
Specific genomic functions are dictated by macromolecular complexes (MCs) containing multiple proteins. Affinity purification of these complexes, often using antibodies, followed by mass spectrometry (MS) has revolutionized our ability to identify the composition of MCs. However, conventional immunoprecipitations suffer from contaminating antibody/serum-derived peptides that limit the sensitivity of detection for low-abundant interacting partners using MS. Here, we present AptA-MS (aptamer affinity-mass spectrometry), a robust strategy primarily using a specific, high-affinity RNA aptamer against Green Fluorescent Protein (GFP) to identify interactors of a GFP-tagged protein of interest by high-resolution MS. Utilizing this approach, we have identified the known molecular chaperones that interact with human Heat Shock Factor 1 (HSF1), and observed an increased association with several proteins upon heat shock, including translation elongation factors and histones. HSF1 is known to be regulated by multiple post-translational modifications (PTMs), and we observe both known and new sites of modifications on HSF1. We show that AptA-MS provides a dramatic target enrichment and detection sensitivity in evolutionarily diverse organisms and allows identification of PTMs without the need for modification-specific enrichments. In combination with the expanding libraries of GFP-tagged cell lines, this strategy offers a general, inexpensive, and high-resolution alternative to conventional approaches for studying MCs.
Asunto(s)
Aptámeros de Nucleótidos/química , Factores de Transcripción del Choque Térmico/química , Sustancias Macromoleculares/aislamiento & purificación , Espectrometría de Masas , Aptámeros de Nucleótidos/genética , Proteínas Fluorescentes Verdes/genética , Factores de Transcripción del Choque Térmico/genética , Histonas/química , Humanos , Inmunoprecipitación , Sustancias Macromoleculares/química , Chaperonas Moleculares/química , Chaperonas Moleculares/genética , Péptidos/química , Unión Proteica , Procesamiento Proteico-PostraduccionalRESUMEN
Heat shock factors (HSFs) play crucial roles in various plant stress responses. However, the current knowledge about HSFs in cassava, an important crop, is still insufficient. In this research, we identified 32 cassava HSF genes (MeHSFs) and clustered them into three groups (A, B, C) based on phylogenetic analysis and structural characteristics. Conserved motif analyses showed that MeHSFs display domains characteristic to HSF transcription factors. Gene structure analyses suggested that 29 MeHSFs contained only two exons. All identified 32 cassava MeHSFs were distributed on 13 chromosomes. Their expression profiles revealed that the different MeHSFs were expressed differentially in different tissues, most high expression genes belonged to group A. The similar MeHSFs were up-regulated after treatment with both PEG and abscisic acid (ABA), which implied that these MeHSFs may participate in resistance to simulated drought stress associated with the ABA signaling pathway. In addition, several MeHSFs were induced during postharvest physiological deterioration (PPD) in cassava. Our results provided basic but important knowledge for future gene function analysis of MeHSFs toward efforts in improving tolerance to abiotic stress and PPD in cassava.
Asunto(s)
Sequías , Genes de Plantas , Factores de Transcripción del Choque Térmico/biosíntesis , Manihot/genética , Proteínas de Plantas/biosíntesis , Estrés Fisiológico/genética , Ácido Abscísico/farmacología , Secuencias de Aminoácidos , Secuencia de Aminoácidos , Mapeo Cromosómico , Cromosomas de las Plantas/genética , Exones/genética , Almacenamiento de Alimentos , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Factores de Transcripción del Choque Térmico/química , Factores de Transcripción del Choque Térmico/genética , Intrones/genética , Manihot/metabolismo , Especificidad de Órganos , Filogenia , Proteínas de Plantas/química , Proteínas de Plantas/genética , Raíces de Plantas/metabolismo , Polietilenglicoles/farmacología , Homología de Secuencia de Aminoácido , Especificidad de la EspecieRESUMEN
The heat shock response is a universal transcriptional response to proteotoxic stress orchestrated by heat shock transcription factor Hsf1 in all eukaryotic cells. Despite over 40 years of intense research, the mechanism of Hsf1 activity regulation remains poorly understood at the molecular level. In metazoa, Hsf1 trimerizes upon heat shock through a leucine-zipper domain and binds to DNA. How Hsf1 is dislodged from DNA and monomerized remained enigmatic. Here, using purified proteins, we demonstrate that unmodified trimeric Hsf1 is dissociated from DNA in vitro by Hsc70 and DnaJB1. Hsc70 binds to multiple sites in Hsf1 with different affinities. Hsf1 trimers are monomerized by successive cycles of entropic pulling, unzipping the triple leucine-zipper. Starting this unzipping at several protomers of the Hsf1 trimer results in faster monomerization. This process directly monitors the concentration of Hsc70 and DnaJB1. During heat shock adaptation, Hsc70 first binds to a high-affinity site in the transactivation domain, leading to partial attenuation of the response, and subsequently, at higher concentrations, Hsc70 removes Hsf1 from DNA to restore the resting state.
Asunto(s)
ADN , Proteínas del Choque Térmico HSC70 , Factores de Transcripción del Choque Térmico , Multimerización de Proteína , Animales , Línea Celular , ADN/química , ADN/genética , ADN/metabolismo , Proteínas del Choque Térmico HSC70/química , Proteínas del Choque Térmico HSC70/genética , Proteínas del Choque Térmico HSC70/metabolismo , Proteínas del Choque Térmico HSP40/química , Proteínas del Choque Térmico HSP40/genética , Proteínas del Choque Térmico HSP40/metabolismo , Factores de Transcripción del Choque Térmico/química , Factores de Transcripción del Choque Térmico/genética , Factores de Transcripción del Choque Térmico/metabolismo , Humanos , Ratones , Ratones Noqueados , Dominios ProteicosRESUMEN
Heat shock transcription factors (Hsfs) play an important role in regulating heat stress response in plants. Our previous study found that there were 82 non-redundant Hsfs in wheat, 18 of which belonged to subclass A2. In this study, we cloned an A2 member, TaHsfA2-1, which encoded a protein of 346 amino acid residues in wheat. The fusion protein TaHsfA2-1-GFP was localized in the nucleus under normal growth conditions. TaHsfA2-1 was expressed in nearly all the measured tissues, most highly in mature leaves. The expression level of TaHsfA2-1 can be enhanced by heat stress, PEG stress, and signal molecules such as H2O2 and SA. Yeast cells transformed with TaHsfA2-1 improved thermotolerance compared to those with the empty vector. TaHsfA2-1-overexpressing Arabidopsis displayed a better growth state with more green leaves than wild-type seedlings after heat stress. Accordingly, the chlorophyll content and survival rate in the transgenic lines were higher than in the wild type, and relative conductivity in the transgenic lines was lower than in the wild type. Further research found that TaHsfA2-1-overexpressing Arabidopsis up-regulated the expression of some heat shock protein genes (Hsps) compared to wild type after heat stress. These results suggested that TaHsfA2-1 is a new gene that improves thermotolerance in plants by mediating the expression of Hsps. A functional gene was provided for molecular breeding in the subsequent research.
Asunto(s)
Regulación de la Expresión Génica de las Plantas/fisiología , Factores de Transcripción del Choque Térmico/genética , Proteínas de Plantas/genética , Termotolerancia/genética , Triticum/fisiología , Secuencia de Aminoácidos , Arabidopsis/genética , Arabidopsis/fisiología , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Factores de Transcripción del Choque Térmico/química , Factores de Transcripción del Choque Térmico/metabolismo , Filogenia , Proteínas de Plantas/metabolismo , Plantas Modificadas Genéticamente/genética , Plantas Modificadas Genéticamente/fisiología , Plantones/genética , Plantones/fisiología , Alineación de Secuencia , Triticum/genéticaRESUMEN
The heat shock factors are important as they are master regulator of heat shock response. There are only few mammalian HSFs which have been characterized, namely HSF-1, HSF-2, HSF-4 and HSF-5. The present study was aimed to clone and sequence characterize the partial open reading frames (ORFs) of HSF-2 and HSF-5 gene from cDNA isolated from testicular tissue of sheep (Macheri) and goat (Beetal). The partial ORFs of HSF-2 gene was observed to be 1627 bp in sheep and 1179 bp in goat and for HSF-5 it is 1137 bp in sheep and 1027 bp in goat. HSF-2 and HSF-5 encode a putative protein of 593 and 461 amino acid in goat and 568 and 553 amino acid in sheep, respectively. Phylogenetic analysis between the different orthologs suggested that these proteins are conserved from bovine to humans as well as in other mammals. Further, domain analyses using PredictNLS, MARCOIL and NetNES revealed that the members of HSF-2 protein orthologs contained all major domains, i.e., DNA-binding domain (DBD) and oligomerization domain (HR-A/B, and HR-C). The 3D structure of sheep and goat HSF-2 protein was predicted using SWISS-MODEL, which showed similar confirmation with the human HSF-2 protein sequence showing functional similarity between them.
Asunto(s)
Cabras/genética , Factores de Transcripción del Choque Térmico/química , Factores de Transcripción del Choque Térmico/genética , Oveja Doméstica/genética , Animales , Secuencia Conservada , Factores de Transcripción del Choque Térmico/clasificación , Humanos , Masculino , Filogenia , Conformación Proteica , Dominios Proteicos , Testículo/químicaRESUMEN
Among the significant transcription factors (TFs), HSF proteins play pivotal roles in the regulation of hormonal signal transduction and different abiotic stress (AbS) responses. Hence considering its importance, global omics expression analysis of HSF candidates was performed in rice (OsHSF). The current study identified 25 HSF family members and physically plotted them against the rice genome. These proteins were systematically analyzed for their physicochemical features, organization and expression signatures. Further, heatmap of both spatio-temporal and global plant hormones revealed the developmental tissues and hormone specific expression profiling of these genes respectively. Comparative genome mapping between OsHSF players in interrelated C4 grass species revealed the chromosome level synteny. Signalome analysis revealed the protein - protein interactions of OsHSF. Expression profiling of key players in response to stresses exhibited the new involvement in combined AbS (CAbS) responses. Our results are significantly valuable to decipher their functional analysis of CAbS tolerant in rice.
Asunto(s)
Factores de Transcripción del Choque Térmico/genética , Oryza/genética , Proteínas de Plantas/genética , Estrés Fisiológico/genética , Mapeo Cromosómico , Simulación por Computador , Expresión Génica/efectos de los fármacos , Ontología de Genes , Marcadores Genéticos , Genómica , Factores de Transcripción del Choque Térmico/química , Factores de Transcripción del Choque Térmico/clasificación , Factores de Transcripción del Choque Térmico/metabolismo , Familia de Multigenes , Oryza/efectos de los fármacos , Oryza/metabolismo , Filogenia , Reguladores del Crecimiento de las Plantas/farmacología , Proteínas de Plantas/química , Proteínas de Plantas/clasificación , Proteínas de Plantas/metabolismo , Poaceae/genética , Mapeo de Interacción de Proteínas , SinteníaRESUMEN
It is generally believed that due to evolutionary differences and adaptation to tropical conditions, Indian native cattle has superior heat tolerant ability than Bos taurus cattle. In the present study, 3'-UTR of two most important heat responsive genes i.e., heat shock protein 70.1 (HSP70.1) and heat shock factor- 1 (HSF-1) were sequence characterized in different breeds of Indian native cattle to identify the variations and miRNA binding sites. In addition, the impact of heat stress was assessed in a total of 57 PBMCs samples of native Sahiwal cows (Bos indicus), exotic Holstein cows (Bos taurus) and Murrah buffaloes (Bubalus bubalis) using various cellular parameters like cell viability, cytotoxicity and apoptosis. Further, expression profile of 12 heat responsive miRNAs were also evaluated in unstressed and stressed PBMCs to understand post transcriptional changes in native cows, exotic cows and Murrah buffaloes. The sequence data showed 3'-UTR of HSP70.1 gene of Indian cattle to be exactly similar to Bos taurus with no miRNA binding site. Whereas, sequencing of 3'-UTR of HSF-1 gene revealed 3 SNPs at positions G1762T; C1811T and C1983T with 7 well conserved miRNA binding sites. The impact of heat stress on various cellular parameters in terms of cell viability, cytotoxicity and apoptosis was highest in PBMCs of Holstein cows followed by Murrah buffaloes and Sahiwal cows. Further, in contrast to Holstein Frisian cows and Murrah buffaloes, the expression pattern of 12 heat responsive miRNAs, in heat stressed PBMCs of Sahiwal cows were quite distinct. There was a significant (p < 0.05) induction in expression of most of the miRNAs after heat stress in PBMCs of Sahiwal cows followed by a rapid decline. The distinct cellular response and pattern of miRNA expression across cattle types and buffaloes might be influencing their PBMCs tolerance level to heat stress.
Asunto(s)
Perfilación de la Expresión Génica/veterinaria , Factores de Transcripción del Choque Térmico/química , Leucocitos Mononucleares/química , MicroARNs/genética , Análisis de Secuencia de ADN/veterinaria , Regiones no Traducidas 3' , Animales , Secuencia de Bases , Sitios de Unión , Búfalos , Bovinos , Secuencia Conservada , Regulación de la Expresión Génica , Proteínas HSP70 de Choque Térmico/química , Proteínas HSP70 de Choque Térmico/genética , Factores de Transcripción del Choque Térmico/genética , Respuesta al Choque Térmico , Polimorfismo de Nucleótido SimpleRESUMEN
Elevated temperatures activate a heat shock response (HSR) to protect cells from the pathological effects of protein mis-folding, cellular mis-organization, organelle dysfunction and altered membrane fluidity. This response includes activation of the conserved transcription factor heat shock factor 1 (HSF-1), which binds heat shock elements (HSEs) in the promoters of genes induced by heat shock (HS). The upregulation of protein-coding genes (PCGs), such as heat shock proteins and cytoskeletal regulators, is critical for cellular survival during elevated temperatures. While the transcriptional response of PCGs to HS has been comprehensively analyzed in a variety of organisms, the effect of this stress on the expression of non-coding RNAs (ncRNAs) has not been systematically examined. Here we show that in Caenorhabditis elegans HS induces up- and downregulation of specific ncRNAs from multiple classes, including miRNA, piRNA, lincRNA, pseudogene and repeat elements. Moreover, some ncRNA genes appear to be direct targets of the HSR, as they contain HSF-1 bound HSEs in their promoters and their expression is regulated by this factor during HS. These results demonstrate that multiple ncRNA genes respond to HS, some as direct HSF-1 targets, providing new candidates that may contribute to organismal survival during this stress.
Asunto(s)
Caenorhabditis elegans/genética , Factores de Transcripción del Choque Térmico/genética , ARN no Traducido/genética , Transcriptoma/genética , Animales , Proteínas de Caenorhabditis elegans/genética , Proteínas de Unión al ADN/genética , Regulación de la Expresión Génica/genética , Factores de Transcripción del Choque Térmico/química , Respuesta al Choque Térmico/genética , Regiones Promotoras Genéticas , Activación Transcripcional/genéticaRESUMEN
Oncogenesis involves continuous genetic alterations that lead to compromised cellular integrity and immortal cell fate. The cells remain under excessive stress due to endo- and exogenous influences. Human Satellite III long noncoding RNA (SatIII lncRNA) is a key regulator of the global cellular stress response, although its function is poorly explained in cancers. The principal regulator of cancer meshwork is tumor protein p53, which if altered may result in chemoresistance. The heat shock factor 1 (HSF1) being a common molecule between the oncogenic control and global cellular stress acts as an oncogene as well as transcribes SatIII upon heat shock. This prompted us to determine the structure of SatIII RNA and establish the association between SatIII-HSF1-p53. We determined the most stable structure of SatIII RNA with the least energy of - 115.7 kcal/mol. Also, we observed a possible interaction of p53 with SatIII and HSF1 using support vector machine (SVM) algorithm for predicting RNA-protein interaction (RPI). Further, we employ the STRING database to understand if p53 is an interacting component of the nuclear stress bodies (nSBs). A precise inference was drawn from molecular docking which confirmed the interaction of SatIII-HSF1-p53, where a mutated p53 resulted in an altered DNA-binding property with the SatIII molecule. This study being first of its kind infers p53 to be a possible integral component of the nSBs, which may regulate cellular stress response during cancer progression in the presence of HSF1 and SatIII. An extended research on the regulations of SatIII and p53 may open new avenues in the field of apoptosis in cancer and the early approach of molecular targeting.
Asunto(s)
Carcinogénesis/patología , Núcleo Celular/genética , Factores de Transcripción del Choque Térmico/metabolismo , ARN Largo no Codificante/metabolismo , Satélite de ARN/metabolismo , Estrés Fisiológico , Proteína p53 Supresora de Tumor/metabolismo , Carcinogénesis/genética , Carcinogénesis/metabolismo , Células HeLa , Factores de Transcripción del Choque Térmico/química , Factores de Transcripción del Choque Térmico/genética , Respuesta al Choque Térmico , Humanos , Simulación del Acoplamiento Molecular , Conformación de Ácido Nucleico , Conformación Proteica , ARN Largo no Codificante/química , ARN Largo no Codificante/genética , Satélite de ARN/química , Satélite de ARN/genética , Transcripción Genética , Proteína p53 Supresora de Tumor/química , Proteína p53 Supresora de Tumor/genéticaRESUMEN
Heat shock transcription factors (Hsfs), which function as the activator of heat shock proteins (Hsps), play multiple roles in response to environmental stress and the development of plants. The Hsf family had experienced gene expansion via whole-genome duplication from a single cell algae to higher plants. However, how the Hsf gene family went through evolutionary divergence after genome duplication is unknown. As a model wood species, Populus trichocarpa is widely distributed in North America with various ecological and climatic environments. In this study, we used P. trichocarpa as materials and identified the expression divergence of the PtHsf gene family in developmental processes, such as dormant bud formation and opening, catkins development, and in response to environments. Through the co-expression network, we further discovered the divergent co-expressed genes that related to the functional divergence of PtHsfs. Then, we studied the alternative splicing events, single nucleotide polymorphism distribution and tertiary structures of members of the PtHsf gene family. In addition to expression divergence, we uncovered the evolutionary divergence in the protein level which may be important to new function formations and for survival in changing environments. This study comprehensively analyzed the evolutionary divergence of a member of the PtHsf gene family after genome duplication, paving the way for further gene function analysis and genetic engineering.
Asunto(s)
Factores de Transcripción del Choque Térmico , Proteínas de Plantas , Populus/genética , Empalme Alternativo , Evolución Biológica , Duplicación de Gen , Regulación de la Expresión Génica de las Plantas , Genes Duplicados , Genes de Plantas , Genoma de Planta , Factores de Transcripción del Choque Térmico/química , Factores de Transcripción del Choque Térmico/genética , Familia de Multigenes , América del Norte , Filogenia , Proteínas de Plantas/química , Proteínas de Plantas/genética , Polimorfismo de Nucleótido SimpleRESUMEN
Global warming has profound effects on plant growth and fitness. Plants have evolved sophisticated epigenetic machinery to respond quickly to heat, and exhibit transgenerational memory of the heat-induced release of post-transcriptional gene silencing (PTGS). However, how thermomemory is transmitted to progeny and the physiological relevance are elusive. Here we show that heat-induced HEAT SHOCK TRANSCRIPTION FACTOR A2 (HSFA2) directly activates the H3K27me3 demethylase RELATIVE OF EARLY FLOWERING 6 (REF6), which in turn derepresses HSFA2. REF6 and HSFA2 establish a heritable feedback loop, and activate an E3 ubiquitin ligase, SUPPRESSOR OF GENE SILENCING 3 (SGS3)-INTERACTING PROTEIN 1 (SGIP1). SGIP1-mediated SGS3 degradation leads to inhibited biosynthesis of trans-acting siRNA (tasiRNA). The REF6-HSFA2 loop and reduced tasiRNA converge to release HEAT-INDUCED TAS1 TARGET 5 (HTT5), which drives early flowering but attenuates immunity. Thus, heat induces transmitted phenotypes via a coordinated epigenetic network involving histone demethylases, transcription factors, and tasiRNAs, ensuring reproductive success and transgenerational stress adaptation.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Factores de Transcripción del Choque Térmico/metabolismo , Histona Demetilasas/metabolismo , Ubiquitina-Proteína Ligasas/metabolismo , Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/genética , Desmetilación , Flores/metabolismo , Regulación de la Expresión Génica de las Plantas , Redes Reguladoras de Genes , Factores de Transcripción del Choque Térmico/química , Factores de Transcripción del Choque Térmico/genética , Proteínas Nucleares/metabolismo , Regiones Promotoras Genéticas , Unión Proteica , Interferencia de ARN , ARN Interferente Pequeño/genética , ARN Interferente Pequeño/metabolismo , Temperatura , Factores de Transcripción/metabolismoRESUMEN
Heat shock factors (HSFs) are the main transcriptional regulators of the heat shock response and indispensable for maintaining cellular proteostasis. HSFs mediate their protective functions through diverse genetic programs, which are composed of genes encoding molecular chaperones and other genes crucial for cell survival. The mechanisms that are used to tailor HSF-driven proteostasis networks are not yet completely understood, but they likely comprise from distinct combinations of both genetic and proteomic determinants. In this review, we highlight the versatile HSF-mediated cellular functions that extend from cellular stress responses to various physiological and pathological processes, and we underline the key advancements that have been achieved in the field of HSF research during the last decade.
Asunto(s)
Factores de Transcripción del Choque Térmico/metabolismo , Proteostasis , Animales , Factores de Transcripción del Choque Térmico/química , Humanos , Conformación Proteica , Procesamiento Proteico-PostraduccionalRESUMEN
Heat shock factor-1 (HSF1) protects neurons from death caused by the accumulation of misfolded proteins by stimulating the transcription of genes encoding heat shock proteins (HSPs). This stimulatory action depends on the association of trimeric HSF1 to sequences within HSP gene promoters. However, we recently described that HSF-AB, a mutant form of HSF1 that is incapable of either homo-trimerization, association with HSP gene promoters, or stimulation of HSP expression, protects neurons just as efficiently as wild-type HSF1 suggesting an alternative neuroprotective mechanism that is activated by HSF1. To gain insight into the mechanism by which HSF1 and HSF1-AB protect neurons, we used RNA-Seq technology to identify transcriptional alterations induced by these proteins in either healthy cerebellar granule neurons (CGNs) or neurons primed to die. When HSF1 was ectopically-expressed in healthy neurons, 1,211 differentially expressed genes (DEGs) were identified with 1,075 being upregulated. When HSF1 was expressed in neurons primed to die, 393 genes were upregulated and 32 genes were downregulated. In sharp contrast, HSF1-AB altered expression of 13 genes in healthy neurons and only 6 genes in neurons under apoptotic conditions, suggesting that the neuroprotective effect of HSF1-AB may be mediated by a non-transcriptional mechanism. We validated the altered expression of 15 genes by QPCR. Although other studies have conducted RNA-Seq analyses to identify HSF1 targets, our study performed using primary neurons has identified a number of novel targets that may play a special role in brain maintenance and function.
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Corteza Cerebelosa/citología , Perfilación de la Expresión Génica/métodos , Redes Reguladoras de Genes , Factores de Transcripción del Choque Térmico/química , Factores de Transcripción del Choque Térmico/genética , Animales , Apoptosis , Células Cultivadas , Corteza Cerebelosa/química , Regulación de la Expresión Génica , Células HEK293 , Factores de Transcripción del Choque Térmico/metabolismo , Humanos , Mutación , Neuronas/química , Neuronas/citología , Regiones Promotoras Genéticas , Mapas de Interacción de Proteínas , Multimerización de Proteína , Ratas , Análisis de Secuencia de ARN/métodosRESUMEN
Targeted therapy is an emerging paradigm in the development of next-generation anticancer drugs. Heat shock factor 1 (HSF1) has been identified as a promising drug target because it regulates several pathways responsible for cancer cell growth, metastasis, and survival. Studies have clearly demonstrated that HSF1 is an effective drug target. Herein, we provide a concise yet comprehensive and integrated overview of progress in developing small molecule inhibitors of HSF1 as next-generation anticancer chemotherapeutics while critically evaluating their potential and challenges. We believe that this review will provide a better understanding of important concepts helpful for outlining the strategy to develop new chemotherapeutic agents with promising anticancer activities by targeting HSF1.
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Antineoplásicos/farmacología , Factores de Transcripción del Choque Térmico/antagonistas & inhibidores , Factores de Transcripción del Choque Térmico/metabolismo , Terapia Molecular Dirigida , Neoplasias/metabolismo , Transducción de Señal/efectos de los fármacos , Animales , Antineoplásicos/química , Antineoplásicos/uso terapéutico , Productos Biológicos/química , Productos Biológicos/farmacología , Productos Biológicos/uso terapéutico , Factores de Transcripción del Choque Térmico/química , Humanos , Neoplasias/tratamiento farmacológico , Profármacos/química , Profármacos/farmacología , Profármacos/uso terapéutico , Relación Estructura-ActividadRESUMEN
Dysfunction of HSF4 is associated with congenital cataracts. HSF4 transcription activity is turned on and regulated by phosphorylation during early postnatal lens development. Our previous data suggested that mutation HSF4b/S299A can upregulate HSF4 transcription activity in vitro, but the biological significance of posttranslational modification on HSF4/S299 during lens development remains unclear. Here, we found that the mutation HSF4/S299A can upregulate the expression of HSP25 and alpha B-crystallin at both protein and mRNA levels in mouse the lens epithelial cell line, but HSF4/S299D does not. Using the rabbit polyclonal antibody against phospho-S299 of HSF4, we found that EGF and ectopic expression of MEK1 can increase the phosphorylation of HSF4/S299 and induce HSF4 sumoylation, and these effects are inhibited by U0126. ERK1/2 can phosphorylate the S299 in HSF4/wt but not in HSF4/S299A in the in vitro kinase assay. Functionally, ectopic MEK1 can inhibit HSF4-controled alpha B-crystallin expression but has less effect on HSF4/S299A. EGF can upregulate phospho-HSF4/S299 and downregulate alpha B-crystallin expression in P3 mouse lens, and this downregulation is suppressed by U0126. During mouse lens development, phosphorylation of HSF4/S299 is downregulated in P3 lens and upregulated in P7 and P14 lens. However, in 2 months old lens, both phosphorylation of HSF4/S299 and total HSF4 protein are decreased. Interestingly, ERK1/2 activity is lower in P3 lens than in P7 and P14 lens, which is in line with the phosphorylation of HSF4/S299. Taken together, our data demonstrate that HSF4/299 is a phosphorylation target of MEK1-ERK1/2, and phosphorylation of S299 is responsible for tuning down HSF4 transcription activity during postnatal lens development.
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Factores de Transcripción del Choque Térmico/genética , Cristalino/metabolismo , Sistema de Señalización de MAP Quinasas , Sustitución de Aminoácidos , Animales , Células Cultivadas , Regulación hacia Abajo , Técnicas de Inactivación de Genes , Proteínas de Choque Térmico HSP27/genética , Factores de Transcripción del Choque Térmico/química , Factores de Transcripción del Choque Térmico/deficiencia , Factores de Transcripción del Choque Térmico/metabolismo , Proteínas de Choque Térmico/genética , Humanos , Cristalino/crecimiento & desarrollo , Ratones , Chaperonas Moleculares , Proteínas de Neoplasias/genética , Fosforilación , Mutación Puntual , ARN Mensajero/genética , ARN Mensajero/metabolismo , Serina/metabolismo , Transcripción Genética , Regulación hacia Arriba , Cadena B de alfa-Cristalina/genéticaRESUMEN
Heat transcription factors (Hsfs) belong to a large gene family classified into A, B, and C groups, with classes A and B Hsfs being well-characterized and known for their roles in plant tolerance to abiotic stresses. The functions and roles of Class C Hsfs are not well-documented. The objectives of this study were to characterize a class C Hsf gene (FaHsfC1b) cloned from tall fescue (Festuca arundinacea), a perennial grass species, and to determine the physiological functions of FaHsfC1b in regulating heat tolerance by overexpressing FaHsfC1b in Arabidopsis thaliana. Full length cDNA of FaHsfC1b was cloned and the sequence alignment showed that it had high similarity to OsHsfC1b with typical DNA binding domain, hydrophobic oligomerization domain, and a nucleus localization signal. Transient expression with FaHsfC1b-eGFP in protoplasts of Arabidopsis leaves indicated its nucleus localization. qRT-PCR analysis showed that FaHsfC1b responded to heat, osmotic, salt, and cold stress in leaves and roots during 48-h treatment. Physiological analysis showed that FaHsfC1b overexpression enhanced plant survival rate, chlorophyll content, and photochemical efficiency, while it resulted in decreases in electrolyte leakage, H2O2 and O2- content under heat stress. qRT-PCR showed that endogenous HsfC1 was induced in transgenic plants and the expression levels of heat protection protein genes, including several HSPs, AtGalSyn1, AtRof1, and AtHSA32, as well as ABA-synthesizing gene (NCED3) were significantly upregulated in transgenic plants overexpressing FaHsfC1b under heat stress. Our results first demonstrate that HsfC1b plays positive roles in plant tolerance to heat stress in association with the induction and upregulation of heat-protective genes. HsfC1b may be used as a candidate gene for genetic modification of cool-season plant species for improving heat tolerance.