Heliconia rostrata rhizomes mitigate chemical-induced liver injury by debilitating oxidative stress in HepG2 cells and rats.

ETHNOPHARMACOLOGICAL RELEVANCE: Heliconia rostrata Ruiz and Pav. belongs to the family Heliconiaceae. Plant was traditionally used to cure jaundice, intestinal pain, diabetes and hypertension. AIM OF THE EXPERIMENT: Present study evaluated hepatoprotective efficacy of ethanol (REE) and methanol (RME) extracts of H. rostrata rhizomes in HepG2 cell lines and rats. Antioxidant efficacy of extracts was determined using ex vivo and in vivo methods. MATERIALS AND METHODS: Before conducting efficacy studies, safety of REE and RME was established using toxicity studies which included Oral acute-fixed dose toxicity using OECD TG420, 28-days repeated dose oral toxicity by OECD TG407 and cytotoxicity studies by brine shrimp lethality (BSL) bioassay and MTT assay taking HepG2 cell line. Ex vivo (Extracts: 0-250 µg/ml) and in vivo (Extracts: 50, 100 and 200 mg/kg) antioxidant studies were performed on fresh goat liver and rats (N = 45) of either sex, respectively. In vitro hepatoprotective efficacy of extracts was evaluated against ethanol induced toxicity in HepG2 cell line. In vivo study was performed at 50, 100 and 200 mg/kg/day doses in rats by CCl4-induced hepatotoxicity study. RESULTS: No mortality was observed during single and repeated dose toxicity studies. 50% lethal dose >2000 mg/kg, confirmed category 5 toxicity level of extracts, according to Globally Harmonized System. No signs of toxicity and treatment or dose related changes recorded in rats under repeated dose toxicity study. No-observed-adverse effect-level of 200 mg/kg/day was observed for both extracts. Median lethal concentration of REE and RME were 1291.30 and 1045.89 µg/ml, respectively in BSL bioassay and 50% cytotoxicity concentration >1000 µg/ml was obtained for both extracts from MTT assay. Calculated 50% inhibitory concentration and median effective dose of extracts obtained from different antioxidant assays in ex vivo and in vivo antioxidant studies, respectively indicated REE has more antioxidant efficacy than RME. In in vitro hepatoprotective efficacy study, extracts demonstrated dose dependent protection against ethanol induced hepatotoxicity. At 400 µg/ml, REE and RME demonstrated percentage protection of 65.53% and 57.98%, respectively. Results of liver function test and histopathological evaluation of liver in in vivo hepatoprotective study confirmed dose dependent protection provided by the extracts against CCl4 -induced hepatotoxicity. CONCLUSION: Both REE and RME were found safe to be considered for therapeutic uses. Both REE and RME were found to exhibit antioxidant efficacy in ex vivo and in vivo models. Results ascertained that H. rostrata rhizomes possess significant hepatoprotective potency.

Vertical flow constructed wetland planted with Heliconia psittacorum used as decentralized post-treatment of anaerobic effluent in Southern Brazil.

In order to investigate the potential of vertical flow constructed wetlands (VFCWs), a 24.5 m2 surface area filled with gravel and planted with Heliconia psittacorum, was implemented as an experimental system for domestic wastewater treatment in southern Brazil. The aims of the study were to (i) evaluate the performance of a full scale system septic tank (ST) and VFCW for decentralized domestic wastewater treatment, and (ii) identify the influence of Heliconia for hydraulic characteristics and consequent performance of the VFCW. The applied load rates in the VFCW were, on average, 10 g biochemical oxygen demand (BOD) m-2 day-1, 4 g NH4+-N m-2 day-1 and a hydraulic loading rate (HLR) of 60 mm day-1. Physicochemical analyses of wastewater treatment were carried out through grab sample methodology, for 5 months. Additionally, hydrodynamic tests were performed during plant development using rhodamine. The study demonstrated the viability of the system for decentralized wastewater treatment in Brazil, with average removal efficiencies of 78% for chemical oxygen demand (COD) and 84% for total suspended solids (TSS), according to national effluent discharge regulations. In spite of media's large size, the VFCW achieved good average removal efficiencies. The tracer test results showed that the VFCW average hydraulic detention time increased as plants grew. This may have occurred due to interception of wastewater applied on the macrophyte's leaves as well as a reduction in filter media permeability caused by the spread of roots and rhizomes. These results indicate that the plants played an important role in increasing wastewater contact time in wetlands with high hydraulic conductivity such as a gravel VFCW.

Phytoremediation of Landfill Leachate with Colocasia esculenta, Gynerum sagittatum and Heliconia psittacorum in Constructed Wetlands.

This study assessed the accumulation of Cd (II), Hg (II), Cr (VI) and Pb (II) in Gynerium sagittatum (Gs), Colocasia esculenta (Ce) and Heliconia psittacorum (He) planted in constructed wetlands treating synthetic landfill leachate. Sixteen bioreactors were operated in two experimental blocks. Metal concentrations in the influent and effluent; root, stem, branch and leaves of plants were analysed, as well as COD, N-NH4+, TKN, T, pH, ORP, DO, and EC. Average removal efficiencies of COD, TKN and NH4+-N were 66, 67 and 72%, respectively and heavy metal removal ranged from 92 to 98% in all units. Cr (VI) was not detected in any effluent sample. The bioconcentration factors (BCF) were 10(0) -10(2). The BCF of Cr (VI) was the lowest: 0.59 and 2.5 (L kg(-1)) for Gs and He respectively; whilst Cd (II) had the highest (130-135 L kg(-1)) for Gs. Roots showed a higher metal content than shoots. Translocation factors (TF) were lower, He was the plant exhibiting TFs>1 for Pb (II), Cr (T) and Hg (II) and 0.4-0.9 for Cd (II) and Cr (VI). The evaluated plants demonstrate their suitability for phytoremediation of landfill leachate and all of them can be categorized as metals accumulators.

Application of ultrasound in the dissolution of potential antiophidian compounds from two ethanolics extracts of two species of Heliconias.

This study evaluates the favorable effects of using ultrasound during the dissolution process of ethanolic extracts of Heliconia psittacorum and Heliconia rostrata (Heliconiaceae), a family of plants reported to have antiophidic activities. The extracts were subjected to an ultrasound treatment before incubation with venom, and carbohydrate and protein contents were calculated. The ultrasound-treated extracts delayed the clotting effect of venom by up to 45.59 s compared to the positive control (venom). The metabolites content increased to 296.6% and 61.6% in protein and carbohydrate content, respectively. Ultrasound increases the amount of primary and secondary metabolites (potentially antiophidics) released from the extract into the media, and thereby enhances the anticoagulant activity of these plants against the Bothrops asper (mapaná X) venom.