RESUMEN
BACKGROUND AND AIM: A large genetic effect of a novel gallstone-associated genetic variant, the hepatocyte nuclear factor 4α (HNF4A) rs1800961 polymorphism, has been identified through recent genome-wide association studies. However, this effect has not been validated in Asian populations. We investigated the association between the rs1800961 variant and gallstones among a Taiwanese population. METHODS: A total of 20 405 participants aged between 30 and 70 years voluntarily enrolled in the Taiwan Biobank. Self-report questionnaires, physical examinations, biochemical tests, and genotyping were used for analysis. The association of the HNF4A rs1800961 variant and other metabolic risks with gallstone disease was analyzed using multiple logistic regression models. RESULTS: The minor T allele of HNF4A rs1800961 was associated with an increased risk of gallstone, and the association remained significant even after adjustment for other risk factors including age, body mass index (BMI), diabetes, hyperlipidemia, hypertension, and cigarette smoking (adjusted odds ratio [OR] = 1.90, 95% confidence interval [CI] = 1.31 to 2.75) in male participants. When further stratified by BMI and age, the lithogenic effect was the most significant in male participants with obesity (adjusted OR = 3.55, 95% CI = 1.92 to 6.56) and who were younger (adjusted OR = 2.45, 95% CI = 1.49 to 4.04). CONCLUSION: The novel gallstone-associated HNF4A rs1800961 variant was associated with the risk of gallstone in the Taiwanese men. Screening for the rs1800961 polymorphism may be particularly useful in assessing the risk of gallstone formation in younger or obese men.
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Cálculos Biliares , Humanos , Masculino , Adulto , Persona de Mediana Edad , Anciano , Cálculos Biliares/etiología , Estudio de Asociación del Genoma Completo , Factores de Riesgo , Obesidad/epidemiología , Obesidad/genética , Obesidad/complicaciones , Factores Nucleares del Hepatocito/genética , Factor Nuclear 4 del Hepatocito/genéticaRESUMEN
Chronic kidney disease (CKD) is associated with elevated plasma fibrinogen concentration. However, the underlying molecular mechanism for elevated plasma fibrinogen concentration in CKD patients has not yet been clarified. We recently found that HNF1α was significantly upregulated in the liver of chronic renal failure (CRF) rats, an experimental model of CKD in patients. Given that the promoter region of the fibrinogen gene possesses potential binding sites for HNF1α, we hypothesized that the upregulation of HNF1α can increase fibrinogen gene expression and consequently plasma fibrinogen concentration in the experimental model of CKD. Here, we found the coordinated upregulation of Aα-chain fibrinogen and Hnfα gene expression in the liver and elevated plasma fibrinogen concentrations in CRF rats, compared with pair-fed and control animals. Liver Aα-chain fibrinogen and HNF1α mRNAs levels correlated positively with (a) liver and plasma fibrinogen levels and (b) liver HNF1α protein levels. The positive correlation between (a) liver Aα-chain fibrinogen mRNA level, (b) liver Aα-chain fibrinogen level, and (c) serum markers of renal function suggest that fibrinogen gene transcription is closely related to the progression of kidney disease. Knockdown of Hnfα in the HepG2 cell line by small interfering RNA (siRNA) led to a decrease in fibrinogen mRNA levels. Clofibrate, an anti-lipidemic drug that reduces plasma fibrinogen concentration in humans, decreased both HNF1α and Aα-chain fibrinogen mRNAs levels in (a) the liver of CRF rats and (b) HepG2 cells. The obtained results suggest that (a) an elevated level of liver HNF1α can play an important role in the upregulation of fibrinogen gene expression in the liver of CRF rats, leading to an elevated concentration of plasma fibrinogen, a protein related to the risk of cardiovascular disease in CKD patients, and (b) fibrates can decrease plasma fibrinogen concentration through inhibition of HNF1α gene expression.
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Fibrinógeno , Fallo Renal Crónico , Ratas , Humanos , Animales , Fibrinógeno/genética , Fibrinógeno/metabolismo , Hígado/metabolismo , Fallo Renal Crónico/genética , Fallo Renal Crónico/metabolismo , ARN Mensajero/genética , ARN Interferente Pequeño/metabolismo , Expresión Génica , Factores Nucleares del Hepatocito/genética , Factores Nucleares del Hepatocito/metabolismoRESUMEN
Hepatocyte nuclear factor 4 (HNF4) is essential for glucose homeostasis and lipid metabolism in insects. However, little is known about the role of HNF4 in whiteflies. In the present study, we identified a hepatocyte nuclear factor protein from Bemsia tabaci (Diptera: Drosophilidae) and named it BtabHNF4. The full-length of BtabHNF4 was 3,006 bp, encoding a sequence of 434 amino acids that contains a conserved zinc-finger DNA-binding domain (DBD) and a well-conserved ligand-binding domain (LBD). The temporal and spatial expression showed that BtabHNF4 was highly expressed in the female adult stage and abdominal tissues of B. tabaci. A leaf-mediated RNA interference method was used to explore the function of BtabHNF4 in whiteflies. Our results showed that the knockdown of BtabHNF4 influences the desiccation tolerance, egg production, and egg hatching rate of whiteflies. Additionally, BtabHNF4 silencing significantly inhibited the expression level of vitellogenin. These results expand the function of HNF4 and pave the way for understanding the molecular mechanisms of HNF4 in regulating multiple physiological processes.
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Hemípteros , Femenino , Animales , Hemípteros/genética , Desecación , Interferencia de ARN , Factores Nucleares del Hepatocito/genética , FertilidadRESUMEN
Mutations in the hepatocyte nuclear factor (HNF)1ß gene (HNF1B) cause autosomal dominant tubulointerstitial kidney disease, a rare and heterogeneous disease characterized by renal cysts and/or malformation, maturity-onset diabetes of the young, hypomagnesemia, and hypokalemia. The electrolyte disturbances may develop in the distal part of the nephron, which is important for fine-tuning of Mg2+ and Ca2+ reabsorption. Therefore, we aimed to study the transcriptional network directed by HNF1ß in the distal part of the nephron. We combined HNF1ß chromatin immunoprecipitation-sequencing and mRNA expression data to identify direct targets of HNF1ß in a renal distal convoluted tubule cell line (mpkDCT). Gene Ontology term pathway analysis demonstrated enrichment of cell polarity, cell-cell junction, and cytoskeleton pathways in the dataset. Genes directly and indirectly regulated by HNF1ß within these pathways included members of the apical and basolateral polarity complexes including Crumbs protein homolog 3 (Crb3), partitioning defective 6 homolog-ß (Pard6b), and LLGL Scribble cell polarity complex component 2 (Llgl2). In monolayers of mouse inner medullary collecting duct 3 cells expressing dominant negative Hnf1b, tight junction integrity was compromised, as observed by reduced transepithelial electrical resistance values and increased permeability for fluorescein (0.4 kDa) compared with wild-type cells. Expression of dominant negative Hnf1b also led to a decrease in height (30%) and an increase in surface (58.5%) of cells grown on membranes. Moreover, three-dimensional spheroids formed by cells expressing dominant negative Hnf1b were reduced in size compared with wild-type spheroids (30%). Together, these findings demonstrate that HNF1ß directs a transcriptional network regulating tight junction integrity and cell structure in the distal part of the nephron.NEW & NOTEWORTHY Genetic defects in transcription factor hepatocyte nuclear factor (HNF)1ß cause a heterogeneous disease characterized by electrolyte disturbances, kidney cysts, and diabetes. By combining RNA-sequencing and HNF1ß chromatin immunoprecipitation-sequencing data, we identified new HNF1ß targets that were enriched for cell polarity pathways. Newly discovered targets included members of polarity complexes Crb3, Pard6b, and Llgl2. Functional assays in kidney epithelial cells demonstrated decreased tight junction integrity and a loss of typical cuboidal morphology in mutant Hnf1b cells.
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Redes Reguladoras de Genes , Factores de Transcripción , Ratones , Animales , Factores de Transcripción/metabolismo , Uniones Estrechas/metabolismo , Riñón/metabolismo , Células Epiteliales/metabolismo , Factores Nucleares del Hepatocito/genética , Factores Nucleares del Hepatocito/metabolismo , Electrólitos/metabolismo , Factor Nuclear 1-beta del Hepatocito/genéticaRESUMEN
Hepatitis B virus (HBV) transcription and replication increase progressively throughout postnatal liver development with maximal viral biosynthesis occurring at around 4 weeks of age in the HBV transgenic mouse model of chronic infection. Increasing viral biosynthesis is associated with a corresponding progressive loss of DNA methylation. The loss of DNA methylation is associated with increasing levels of 5-hydroxymethylcytosine (5hmC) residues which correlate with increased liver-enriched pioneer transcription factor Forkhead box protein A (FoxA) RNA levels, a rapid decline in postnatal liver DNA methyltransferase (Dnmt) transcripts, and a very modest reduction in ten-eleven translocation (Tet) methylcytosine dioxygenase expression. These observations are consistent with the suggestion that the balance between active HBV DNA methylation and demethylation is regulated by FoxA recruitment of Tet in the presence of declining Dnmt activity. These changes lead to demethylation of the viral genome during hepatocyte maturation with associated increases in viral biosynthesis. Consequently, manipulation of the relative activities of these two counterbalancing processes might permit the specific silencing of HBV gene expression with the loss of viral biosynthesis and the resolution of chronic HBV infections.IMPORTANCE HBV biosynthesis begins at birth and increases during early postnatal liver development in the HBV transgenic mouse model of chronic infection. The levels of viral RNA and DNA synthesis correlate with pioneer transcription factor FoxA transcript plus Tet methylcytosine dioxygenase-generated 5hmC abundance but inversely with Dnmt transcript levels and HBV DNA methylation. Together, these findings suggest that HBV DNA methylation during neonatal liver development is actively modulated by the relative contributions of FoxA-recruited Tet-mediated DNA demethylation and Dnmt-mediated DNA methylation activities. This mode of gene regulation, mediated by the loss of DNA methylation at hepatocyte-specific viral and cellular promoters, likely contributes to hepatocyte maturation during liver development in addition to the postnatal activation of HBV transcription and replication.
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ADN Viral/metabolismo , Virus de la Hepatitis B/fisiología , Hepatitis B Crónica/virología , Hígado/crecimiento & desarrollo , 5-Metilcitosina/análogos & derivados , 5-Metilcitosina/metabolismo , Animales , Animales Recién Nacidos , ADN (Citosina-5-)-Metiltransferasas/genética , ADN (Citosina-5-)-Metiltransferasas/metabolismo , Metilación de ADN , Replicación del ADN , ADN Viral/biosíntesis , Desmetilación , Dioxigenasas/genética , Dioxigenasas/metabolismo , Modelos Animales de Enfermedad , Regulación del Desarrollo de la Expresión Génica , Regulación Viral de la Expresión Génica , Hepatitis B Crónica/metabolismo , Hepatitis B Crónica/patología , Factores Nucleares del Hepatocito/genética , Factores Nucleares del Hepatocito/metabolismo , Hígado/metabolismo , Hígado/virología , Ratones , Ratones Transgénicos , ARN Viral/biosíntesis , Replicación ViralRESUMEN
BACKGROUND: Hepatocyte nuclear factor-4α (HNF4A) can influence the risk of insulin resistance that is postulated to be an important link between metabolic syndrome (MetS) and testosterone deficiency (TD) in men. AIM: To investigate the relationship between single-nucleotide polymorphisms (SNPs) of HNF4A and the risk of developing MetS and TD in a population of aging Taiwanese men. METHODS: A free health screening of men over 40 years of age was conducted in a medical center in Kaohsiung City, Taiwan. All participants underwent a physical examination, answered a questionnaire on demographics and medical history, completed the Androgen Deficiency in The Aging Male questionnaire to assess clinical symptoms of TD, and provided 20-mL whole blood samples for biochemical, hormonal, and genetic evaluation. MAIN OUTCOME MEASURE: 3 common SNPs (rs11574736, rs1884613, and rs2144908) of HNF4A were selected and identified using a TaqMan 5' allelic discrimination assay. RESULTS: 559 men were enrolled for this study (mean age, 55.8± 4.9 years). Prevalence of TD was significantly higher (P = .031) in subjects with MetS (16.8%) than those without MetS (10.1%). In SNP rs1884613 of HNF4A, subjects with the C allele carried a 1.31- and 1.50-times higher risk of developing MetS and TD, respectively, compared to those with the G allele, after adjusting for potential covariates. In addition, subjects with the CC genotype were exposed to a 1.91- and 2.20-times higher risk of developing MetS and TD, respectively, compared to those with the GG genotype. CLINICAL IMPLICATIONS: Our findings may point to the importance of the role played by insulin resistance in the link between MetS and TD. STRENGTH & LIMITATIONS: Our current work is the first report with adequate sample size to evaluate the role of genetic variants of HNF4A on the risk of both MetS and TD in men. The limitations included subjects enrolled from a free health screening and single measurement of serum testosterone levels. CONCLUSION: The rs1884613 SNP marker of HNF4A is significantly associated with an increased risk for developing both MetS and TD in aging Taiwanese men. Further population-based studies utilizing larger samples of different ethnicities may be needed to confirm these preliminary results. Liu C-C, Lee Y-C, Hung S-P. Hepatocyte Nuclear Factor-4α P2 Promoter Variants Are Associated With the Risk of Metabolic Syndrome and Testosterone Deficiency in Aging Taiwanese Men. J Sex Med 2018;15:1527-1536.
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Disfunción Eréctil/genética , Factores Nucleares del Hepatocito/genética , Síndrome Metabólico/genética , Regiones Promotoras Genéticas/genética , Testosterona/deficiencia , Adulto , Anciano , Envejecimiento , Pueblo Asiatico , Disfunción Eréctil/sangre , Disfunción Eréctil/epidemiología , Genotipo , Humanos , Masculino , Salud del Hombre , Síndrome Metabólico/sangre , Persona de Mediana Edad , Polimorfismo de Nucleótido Simple , Prevalencia , Encuestas y Cuestionarios , Taiwán , Testosterona/sangreRESUMEN
BACKGROUND: Causative mutations cannot be identified in the majority of Asian patients with suspected maturity-onset diabetes of the young (MODY). OBJECTIVES: To elucidate the genetic basis of Japanese patients with MODY-like diabetes and gain insight into the etiology of patients without mutations in the major MODY genes. SUBJECTS: A total of 263 Japanese patients with early-onset, non-obese, MODY-like diabetes mellitus referred to Osaka City General Hospital for diagnosis. METHODS: Mutational analysis of the four major MODY genes (GCK, HNF1A, HNF4A, HNF1B) by Sanger sequencing. Mutation-positive and mutation-negative patients were further analyzed for clinical features. RESULTS: Mutations were identified in 103 (39.2%) patients; 57 mutations in GCK; 29, HNF1A; 7, HNF4A; and 10, HNF1B. Contrary to conventional diagnostic criteria, 18.4% of mutation-positive patients did not have affected parents and 8.2% were in the overweight range (body mass index [BMI] >85th percentile). HOMA-IR at diagnosis was elevated (>2) in 15 of 66 (22.7%) mutation-positive patients. Compared with mutation-positive patients, mutation-negative patients were significantly older (P = 0.003), and had higher BMI percentile at diagnosis (P = 0.0006). Interestingly, maternal inheritance of diabetes was significantly more common in mutation-negative patients (P = 0.0332) and these patients had significantly higher BMI percentile as compared with mutation-negative patients with paternal inheritance (P = 0.0106). CONCLUSIONS: Contrary to the conventional diagnostic criteria, de novo diabetes, overweight, and insulin-resistance are common in Japanese patients with mutation-positive MODY. A significant fraction of mutation-negative patients had features of early-onset type 2 diabetes common in Japanese, and non-Mendelian inheritance needs to be considered for these patients.
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Diabetes Mellitus Tipo 2/genética , Factores Nucleares del Hepatocito/genética , Herencia Materna , Proteínas Serina-Treonina Quinasas/genética , Adolescente , Adulto , Niño , Preescolar , Diabetes Mellitus Tipo 2/epidemiología , Femenino , Quinasas del Centro Germinal , Humanos , Japón/epidemiología , Masculino , Adulto JovenRESUMEN
PURPOSE OF REVIEW: Monogenic diabetes accounts for 1-2% of all diabetes cases, but is frequently misdiagnosed as type 1, type 2, or gestational diabetes. Accurate genetic diagnosis directs management, such as no pharmacologic treatment for GCK-MODY, low-dose sulfonylureas for HNF1A-MODY and HNF4A-MODY, and high-dose sulfonylureas for KATP channel-related diabetes. While diabetes treatment is defined for the most common causes of monogenic diabetes, pregnancy poses a challenge to management. Here, we discuss the key issues in pregnancy affected by monogenic diabetes. RECENT FINDINGS: General recommendations for pregnancy affected by GCK-MODY determine need for maternal insulin treatment based on fetal mutation status. However, a recent study suggests macrosomia and miscarriage rates may be increased with this strategy. Recent demonstration of transplacental transfer of sulfonylureas also raises questions as to when insulin should be initiated in sulfonylurea-responsive forms of monogenic diabetes. Pregnancy represents a challenge in management of monogenic diabetes, where factors of maternal glycemic control, fetal mutation status, and transplacental transfer of medication must all be taken into consideration. Guidelines for pregnancy affected by monogenic diabetes will benefit from large, prospective studies to better define the need for and timing of initiation of insulin treatment.
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Diabetes Mellitus Tipo 2/terapia , Embarazo en Diabéticas/terapia , Diabetes Mellitus Tipo 2/diagnóstico , Diabetes Mellitus Tipo 2/genética , Diabetes Mellitus Tipo 2/fisiopatología , Femenino , Glucoquinasa/genética , Factores Nucleares del Hepatocito/genética , Humanos , Mutación , Canales de Potasio/genética , Embarazo , Embarazo en Diabéticas/diagnóstico , Embarazo en Diabéticas/genética , Embarazo en Diabéticas/fisiopatologíaRESUMEN
Hepatocyte to biliary transdifferentiation has been documented in various models of bile duct injury. In this process, mature hepatocytes transform into mature biliary epithelial cells by acquiring biliary phenotypic markers. Several signaling pathways including PI3 kinase, Notch, Hes1, Sox9, and Hippo are shown to be involved in the process. However, whether Oct4 is involved in hepatocyte to biliary transdifferentiation is unknown. We investigated the role of Oct4 in hepatocyte to biliary transdifferentiation utilizing an in vitro organoid culture system as a model of transdifferentiation. Oct4 was inhibited using adenovirus containing Oct4 shRNA. Hepatocyte-specific HNF-4α and biliary-specific HNF-1ß and CK19 expression were assessed to gauge the extent of transdifferentiation. Oct4 was induced during hepatocyte to biliary transdifferentiation. Oct4 inhibition significantly downregulated the appearance of biliary cells from hepatocytes. This was accompanied by a significant downregulation of signaling pathways including Notch, Sox9, and Hippo. Our findings suggest that Oct4 is crucial for hepatocyte to biliary transdifferentiation and maturation and that it acts upstream of Notch, Sox9, and Hippo signaling in this model. This finding identifies new signaling through Oct4 in plasticity between hepatocytes and biliary epithelial cells, which can be potentially utilized to identify new strategies in chronic biliary diseases.
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Transdiferenciación Celular , Hepatocitos/metabolismo , Factor 3 de Transcripción de Unión a Octámeros/metabolismo , Animales , Conductos Biliares/citología , Células Cultivadas , Factores Nucleares del Hepatocito/genética , Factores Nucleares del Hepatocito/metabolismo , Hepatocitos/citología , Masculino , Factor 3 de Transcripción de Unión a Octámeros/genética , Organoides/citología , Organoides/metabolismo , Ratas , Ratas Endogámicas F344 , Receptores Notch/genética , Receptores Notch/metabolismo , Factor de Transcripción SOX9/genética , Factor de Transcripción SOX9/metabolismo , Transducción de SeñalRESUMEN
The hepatocyte nuclear factors (HNFs) namely HNF1α/ß, FOXA1/2/3, HNF4α/γ and ONECUT1/2 are expressed in a variety of tissues and organs, including the liver, pancreas and kidney. The spatial and temporal manner of HNF expression regulates embryonic development and subsequently the development of multiple tissues during adulthood. Though the HNFs were initially identified individually based on their roles in the liver, numerous studies have now revealed that the HNFs cross-regulate one another and exhibit synergistic relationships in the regulation of tissue development and function. The complex HNF transcriptional regulatory networks have largely been elucidated in rodent models, but less so in human biological systems. Several heterozygous mutations in these HNFs were found to cause diseases in humans but not in rodents, suggesting clear species-specific differences in mutational mechanisms that remain to be uncovered. In this review, we compare and contrast the expression patterns of the HNFs, the HNF cross-regulatory networks and how these liver-enriched transcription factors serve multiple functions in the liver and beyond, extending our focus to the pancreas and kidney. We also summarise the insights gained from both human and rodent studies of mutations in several HNFs that are known to lead to different disease conditions.
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Factores Nucleares del Hepatocito/metabolismo , Hígado/metabolismo , Animales , Diabetes Mellitus Tipo 2/genética , Diabetes Mellitus Tipo 2/metabolismo , Regulación del Desarrollo de la Expresión Génica , Redes Reguladoras de Genes , Factores Nucleares del Hepatocito/química , Factores Nucleares del Hepatocito/genética , Humanos , Riñón/metabolismo , Hígado/crecimiento & desarrollo , Redes y Vías Metabólicas , Mutación , Páncreas/metabolismo , Distribución TisularRESUMEN
Objective: To profile the gene expression changes associated with endoplasmic reticulum stress in INS-1-3 cells induced by thapsigargin (TG) and tunicamycin (TM). Methods: Normal cultured INS-1-3 cells were used as a control. TG and TM were used to induce endoplasmic reticulum stress in INS-1-3 cells. Digital gene expression profiling technique was used to detect differentially expressed gene. The changes of gene expression were detected by expression pattern clustering analysis, gene ontology (GO) function and pathway enrichment analysis. Real time polymerase chain reaction (RT-PCR) was used to verify the key changes of gene expression. Results: Compared with the control group, there were 57 (45 up-regulated, 12 down-regulated) and 135 (99 up-regulated, 36 down-regulated) differentially expressed genes in TG and TM group, respectively. GO function enrichment analyses indicated that the main enrichment was in the endoplasmic reticulum. In signaling pathway analysis, the identified pathways were related with endoplasmic reticulum stress, antigen processing and presentation, protein export, and most of all, the maturity onset diabetes of the young (MODY) pathway. Conclusion: Under the condition of endoplasmic reticulum stress, the related expression changes of transcriptional factors in MODY signaling pathway may be related with the impaired function in islet beta cells.
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Estrés del Retículo Endoplásmico , Factores Nucleares del Hepatocito/genética , Animales , Línea Celular , Retículo Endoplásmico , Transducción de Señal , Tapsigargina , Tunicamicina , Regulación hacia ArribaRESUMEN
Great research effort has been focused on elucidating the contribution of host genetic variability on pharmacological outcomes in cancer. Nuclear receptors have emerged as mediators between environmental stimuli and drug pharmacokinetics and pharmacodynamics. The pregnane X receptor, constitutive androstane receptor and hepatocyte nuclear factors have been reported to regulate transcription of genes that encode drug metabolizing enzymes and transporters. Altered nuclear receptor expression has been shown to affect the metabolism and pharmacological profile of traditional chemotherapeutics and targeted agents. Accordingly, polymorphic variants in these genes have been studied as pharmacogenetic markers of outcome variability. This review summarizes the state of knowledge about the roles played by pregnane X receptor, constitutive androstane receptor and hepatocyte nuclear factor expression and genetics as predictive markers of anticancer drug toxicity and efficacy, which can improve cancer precision medicine.
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Factores Nucleares del Hepatocito/fisiología , Neoplasias/tratamiento farmacológico , Medicina de Precisión , Receptores Citoplasmáticos y Nucleares/fisiología , Receptores de Esteroides/fisiología , Antineoplásicos/efectos adversos , Antineoplásicos/uso terapéutico , Receptor de Androstano Constitutivo , Factores Nucleares del Hepatocito/genética , Humanos , Neoplasias/genética , Receptor X de Pregnano , Receptores Citoplasmáticos y Nucleares/genética , Receptores de Esteroides/genéticaRESUMEN
Maturity-onset diabetes of the young (MODY) is a group of monogenic diseases that results in primary defects in insulin secretion and dominantly inherited forms of nonautoimmune diabetes. Although many genes may be associated with monogenic diabetes, heterozygous mutations in 6 of them are responsible for the majority of cases of MODY. Glucokinase (GCK)-MODY is due to mutations in the glucokinase gene, 3 MODY subtypes are associated with mutations in the hepatocyte nuclear factor (HNF) transcription factors, and 2 others with mutations in ABCC8 and KCNJ11, which encode the subunits of the ATP-dependent potassium channel in pancreatic beta cells. GCK-MODY and HNF1A-MODY are the most common subtypes. The clinical presentation of MODY subtypes has been reported to differ according to the gene involved, and the diagnosis of MODY may be considered in various clinical circumstances. However, except in patients with GCK-MODY whose phenotype is very homogeneous, in most cases the penetrance and expressivity of a given molecular abnormality vary greatly among patients and, conversely, alterations in various genes may lead to similar phenotypes. Moreover, differential diagnosis among more common forms of diabetes may be difficult, particularly with type 2 diabetes. Thus, careful assessment of the personal and family histories of patients with diabetes is mandatory to select those in whom genetic screening is worthwhile. The diagnosis of monogenic diabetes has many consequences in terms of prognosis, therapeutics and family screening.
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Diabetes Mellitus/diagnóstico , Enfermedades Raras/diagnóstico , Adolescente , Niño , Diabetes Mellitus/genética , Diabetes Mellitus/terapia , Diagnóstico Diferencial , Errores Diagnósticos , Femenino , Regulación de la Expresión Génica , Pruebas Genéticas , Glucoquinasa/genética , Factores Nucleares del Hepatocito/genética , Humanos , Masculino , Canales de Potasio de Rectificación Interna/genética , Guías de Práctica Clínica como Asunto , Enfermedades Raras/genética , Enfermedades Raras/terapia , Receptores de Sulfonilureas/genéticaRESUMEN
The embryonic development and carcinogenesis are controlled by many transcription factors. The regulatory systems involved in embryogenesis of an organ are also involved in the tumor development in the same organ. FOX family proteins are transcription factors, which play a key role in these processes. The pioneering factors of the FOXA subfamily act at the very early stages of the embryonic development by interacting with condensed chromatin and thereby enabling the expression of the formerly silent important transcription factors. The role of these factors in tumor development is currently not fully elucidated, although recent studies indicate the important contribution of the FOXA subfamily proteins at the early stages of carcinogenesis. This review is restricted to the role of the FOXA factors in embryogenesis of the pancreas and their significance in the development of the pancreatic ductal adenocarcinoma.
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Transformación Celular Neoplásica , Embrión de Mamíferos , Desarrollo Embrionario , Factores Nucleares del Hepatocito , Proteínas de Neoplasias , Neoplasias Pancreáticas , Animales , Transformación Celular Neoplásica/genética , Transformación Celular Neoplásica/metabolismo , Transformación Celular Neoplásica/patología , Embrión de Mamíferos/metabolismo , Embrión de Mamíferos/patología , Factores Nucleares del Hepatocito/genética , Factores Nucleares del Hepatocito/metabolismo , Humanos , Proteínas de Neoplasias/genética , Proteínas de Neoplasias/metabolismo , Neoplasias Pancreáticas/genética , Neoplasias Pancreáticas/metabolismo , Neoplasias Pancreáticas/patologíaRESUMEN
Planarians can regenerate any missing body part in a process requiring dividing cells called neoblasts. Historically, neoblasts have largely been considered a homogeneous stem cell population. Most studies, however, analyzed neoblasts at the population rather than the single-cell level, leaving the degree of heterogeneity in this population unresolved. We combined RNA sequencing of neoblasts from wounded planarians with expression screening and identified 33 transcription factors transcribed in specific differentiated cells and in small fractions of neoblasts during regeneration. Many neoblast subsets expressing distinct tissue-associated transcription factors were present, suggesting candidate specification into many lineages. Consistent with this possibility, klf, pax3/7, and FoxA were required for the differentiation of cintillo-expressing sensory neurons, dopamine-ß-hydroxylase-expressing neurons, and the pharynx, respectively. Together, these results suggest that specification of cell fate for most-to-all regenerative lineages occurs within neoblasts, with regenerative cells of blastemas being generated from a highly heterogeneous collection of lineage-specified neoblasts.
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Células Madre/citología , Animales , Secuencia de Bases , Diferenciación Celular , Factores Nucleares del Hepatocito/genética , Factores Nucleares del Hepatocito/metabolismo , Factores de Transcripción de Tipo Kruppel/antagonistas & inhibidores , Factores de Transcripción de Tipo Kruppel/genética , Factores de Transcripción de Tipo Kruppel/metabolismo , Datos de Secuencia Molecular , Factores de Transcripción Paired Box/antagonistas & inhibidores , Factores de Transcripción Paired Box/genética , Factores de Transcripción Paired Box/metabolismo , Planarias , Interferencia de ARN , ARN Interferente Pequeño/metabolismo , Regeneración , Células Receptoras Sensoriales/citología , Células Receptoras Sensoriales/metabolismo , Análisis de Secuencia de ARN , Células Madre/metabolismo , Factores de Transcripción/antagonistas & inhibidores , Factores de Transcripción/química , Factores de Transcripción/metabolismoRESUMEN
Diverse aetiologies result in significant deviation from homoeostasis in the kidney, leading to CKD (chronic kidney disease). CKD progresses to end-stage renal disease principally as a result of renal fibrosis, although the molecular mechanisms underlying this fibrotic process are still poorly understood. miRNAs (microRNAs) are a recently discovered family of endogenous short single-stranded RNAs that regulate global gene expression at the post-transcriptional level. The recent findings from our laboratory and others discussed in the present review outline pleiotropic roles for miR-192 in renal homoeostasis and in the fibrotic kidney. We describe miR-192-driven anti-and pro-fibrotic effects via the repression of ZEB1 and ZEB2 (zinc finger E-box-binding homeobox proteins 1 and 2), resulting in changes in extracellular matrix deposition and cell differentiation.
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Riñón/metabolismo , MicroARNs/genética , Animales , Fibrosis/genética , Factores Nucleares del Hepatocito/genética , Factores Nucleares del Hepatocito/metabolismo , Humanos , Riñón/patología , Enfermedades Renales/genética , Enfermedades Renales/metabolismoRESUMEN
miR (microRNA)-192 plays key roles in renal pathological and physiological responses, by repressing targets including Zeb1, Zeb2 and Wnk1. In the present study, we have assessed the regulation of miR-192 expression. We found that TGF-ß1 (transforming growth factor ß1) down-regulates miR-192 and miR-194, co-transcribed in the shared precursor pri-miR (primary miR transcript)-192/194. Luciferase reporter analysis showed constitutive promoter activity within nucleotides +21 to -223. We identified HNF (hepatocyte nuclear factor) and p53 binding sites within this region that were required for constitutive promoter activity, which was decreased by TGF-ß1 through an Alk5-dependent mechanism. TGF-ß1 treatment decreased HNF binding to the miR-194-2/192 promoter, whereas knockdown of HNF-1 inhibited mature miR-192 and miR-194 expression. miR-192, miR-194 and HNF expression were restricted to a defined subset of human tissues including kidney, small intestine, colon and liver. Our results from the present study identify co-ordinated regulation of miR-192 and miR-194, with binding of HNF and p53 transcription factors necessary for activation of transcription, and TGF-ß1-mediated repression through decreased HNF binding to its cognate promoter element.
Asunto(s)
ADN/metabolismo , Regulación hacia Abajo , Factores Nucleares del Hepatocito/metabolismo , MicroARNs/genética , Factor de Crecimiento Transformador beta1/metabolismo , Secuencia de Bases , Células Cultivadas , ADN/genética , Factores Nucleares del Hepatocito/genética , Humanos , Regiones Promotoras Genéticas , Unión Proteica , ARN Interferente Pequeño/genética , Factor de Crecimiento Transformador beta1/genética , Proteína p53 Supresora de Tumor/genética , Proteína p53 Supresora de Tumor/metabolismoRESUMEN
Introducción. El síndrome de Rett (SR) es un trastorno del neurodesarrollo que afecta casi exclusivamente a niñas. Laidentificación de mutaciones en los genes MECP2 y CDKL5 aporta una confirmación genética del diagnóstico clínico. El genFOXG1 se perfila como un nuevo responsable de la variante congénita del SR.Caso clínico. Se describe la primera paciente española con variante atípica (congénita) del SR con mutación en el genFOXG1, y se compara con 12 pacientes publicados en la bibliografía; se proponen criterios clínicos sugestivos de alteracionesen el FOXG1. La paciente fue remitida a los 6 meses por retraso global, hipotonía axial, microcefalia y fenotipo peculiar.Resonancia magnética cerebral: hipoplasia del cuerpo calloso, atrofia frontal y ventriculomegalia. Aparición de estereotipiasmano-boca a los 12 meses que orientan hacia el diagnóstico clínico de variante atípica del SR, forma congénita;mejoría progresiva del contacto visual e interés por el medio. Infecciones respiratorias frecuentes y síndrome de apneaobstructiva del sueño. A los 5 años existe un control parcial del tono axial, prensión manual, buen contacto y balbuceo,sin epilepsia ni alteraciones conductuales. El estudio de MECP2 y deleciones subteloméricas no mostró alteraciones; seidentificaron dos polimorfismos en el gen CDKL5 y una mutación patogénica en el FOXG1 (c.624C>G p.Tyr203X).Conclusiones. El 92% de pacientes con mutaciones en el gen FOXG1 presenta la forma congénita del SR con una gravehipotonía generalizada, microcefalia adquirida precoz (3 a 6 desviaciones estándares) y fenotipo peculiar. Ante undiagnóstico de SR sin alteración en los genes MECP2 y CDKL5, especialmente en la variante congénita, debe investigarse elgen FOXG1. El diagnóstico molecular confirma el diagnóstico clínico y aporta un consejo genético a la familia (AU)
Introduction. Rett syndrome (RS) is a neurodevelopmental disorder that affects girls almost exclusively. The identificationof mutations in the MECP2 and CDKL5 genes offers genetic confirmation of the clinical diagnosis. The FOXG1 gene appearsto be a novel cause of the congenital variant of RS.Case report. We describe the first Spanish patient with the atypical (congenital) variant of RS with mutation of the FOXG1gene and the case is compared with 12 patients previously reported in the literature; clinical criteria that suggest alterationsin FOXG1 are proposed. The patient was referred at the age of 6 months due to overall retardation, axial hypotonia,microcephaly and a peculiar phenotype. Magnetic resonance imaging of the brain revealed hypoplasia of the corpuscallosum, frontal atrophy and ventriculomegaly. The appearance of hand-to-mouth stereotypic movements at 12 monthspointed the clinical diagnosis towards an atypical variant of RS, the congenital form; there was progressive improvementof visual contact and interest in her surroundings. Frequent respiratory infections and obstructive sleep apnoea syndrome.At the age of 5 years there was partial control over the axial tone, grasping with the hands, good contact and babbling,without epilepsy or behavioural disorders. The MECP2 and subtelomeric deletion study did not reveal any alterations; twopolymorphisms were identified in the CDKL5 gene and a pathogenic mutation was found in FOXG1 (c.624C>G p.Tyr203X).Conclusions. It has been shown that 92% of patients with mutations in the FOXG1 gene present the congenital form of RSwith severe generalised hypotonia, early acquired microcephaly (3 to 6 standard deviations) (AU)