RESUMEN
Telocytes, a peculiar cell type, were recently found in vertebrates. Hence this cell system has been reported as ubiquitous in the bodies of mammals and interpreted as an important player in innate immunity and tissue regeneration, it is reasonable to look for it also in invertebrates, that rely their integrity solely by innate immunity. Here we describe, at morphological and functional level, invertebrate telocytes from the body of leech Hirudo medicinalis (Annelida), suggesting how these cells, forming a resident stromal 3D network, can influence or participate in different events. These findings support the concepts that leech telocytes: i) are organized in a cellular dynamic and versatile 3D network likewise the vertebrate counterpart; ii) are an evolutionarily conserved immune-neuroendocrine system; iii) form an immuno-surveillance system of resident cells responding faster than migrating immunocytes recruited in stimulated area; iv) communicate with neighbouring cells directly and indirectly, via cell-cell contacts and soluble molecules secreted by multivesicular bodies; v) present within neo-vessels, share with immunocytes the mesodermal lineage; vi) are involved in regenerative processes. In conclusion, we propose that HmTCs, integrating so different functions, might explain the innate immune memory and can be associated with several aged related diseases.
Asunto(s)
Hirudo medicinalis/citología , Hirudo medicinalis/fisiología , Animales , Biomarcadores/metabolismo , Inmunidad Celular , Microscopía Electrónica de Transmisión , Sistemas Neurosecretores/citología , Cicatrización de HeridasRESUMEN
Recent studies in the field of neuro-tissue engineering have demonstrated the promising effects of aligned contact guidance cue to scaffolds of enhancement and direction of neuronal growth. In vivo, neurons grow and develop neurites in a complex three-dimensional (3D) extracellular matrix (ECM) surrounding. Studies have utilized hydrogel scaffolds derived from ECM molecules to better simulate natural growth. While many efforts have been made to control neuronal growth on 2D surfaces, the development of 3D scaffolds with an elaborate oriented topography to direct neuronal growth still remains a challenge. In this study, we designed a method for growing neurons in an aligned and oriented 3D collagen hydrogel. We aligned collagen fibers by inducing controlled uniaxial strain on gels. To examine the collagen hydrogel as a suitable scaffold for neuronal growth, we evaluated the physical properties of the hydrogel and measured collagen fiber properties. By combining the neuronal culture in 3D collagen hydrogels with strain-induced alignment, we were able to direct neuronal growth in the direction of the aligned collagen matrix. Quantitative evaluation of neurite extension and directionality within aligned gels was performed. The analysis showed neurite growth aligned with collagen matrix orientation, while maintaining the advantageous 3D growth.
Asunto(s)
Colágeno/química , Hirudo medicinalis/citología , Hidrogeles/química , Neuritas/metabolismo , AnimalesRESUMEN
Allograft inflammatory factor-1 (AIF-1) is a 17-kDa cytokine-inducible calcium-binding protein that, in vertebrates, plays an important role in the allograft immune response. Its expression is mostly limited to the monocyte/macrophage lineage. Until recently, AIF-1 was assumed to be a novel molecule involved in inflammatory responses. To clarify this aspect, we have investigated the expression of AIF-1 after bacterial challenge and its potential role in regulating the innate immune response in an invertebrate model, the medicinal leech (Hirudo medicinalis). Analysis of an expressed sequence tag library from the central nervous system of Hirudo revealed the presence of the gene Hmaif-1/alias Hmiba1, showing high homology with vertebrate aif-1. Immunohistochemistry with an anti-HmAIF-1 polyclonal antibody revealed the constitutive presence of this protein in spread CD68(+) macrophage-like cells. A few hours after pathogen (bacterial) injection into the body wall, the amount of these immunopositive cells co-expressing HmAIF-1 and the common leucocyte marker CD45 increased at the injected site. Moreover, the recombinant protein HmAIF-1 induced massive angiogenesis and was a potent chemoattractant for macrophages. Following rHmAIF-1 stimulation, macrophage-like cells co-expressed the macrophage marker CD68 and the surface glycoprotein CD45, which, in vertebrates, seems to have a role in the integrin-mediated adhesion of macrophages and in the regulation of the functional responsiveness of cells to chemoattractants. CD45 is therefore probably involved in leech macrophage-like cell activation and migration towards an inflammation site. We have also examined its potential effect on HmAIF-1-induced signalling.
Asunto(s)
Movimiento Celular/efectos de los fármacos , Proteínas de Unión al ADN/farmacología , Hirudo medicinalis/citología , Hirudo medicinalis/inmunología , Inmunidad Innata/efectos de los fármacos , Macrófagos/citología , Homología de Secuencia de Aminoácido , Animales , Anticuerpos/farmacología , Biomarcadores/metabolismo , Western Blotting , Movimiento Celular/inmunología , Forma de la Célula/efectos de los fármacos , Proteínas de Unión al ADN/química , Hirudo medicinalis/microbiología , Hirudo medicinalis/ultraestructura , Inmunohistoquímica , Antígenos Comunes de Leucocito/metabolismo , Macrófagos/efectos de los fármacos , Macrófagos/inmunología , Proteínas Recombinantes/farmacologíaRESUMEN
BACKGROUND: In invertebrates, the medicinal leech is considered to be an interesting and appropriate model to study neuroimmune mechanisms. Indeed, this non-vertebrate animal can restore normal function of its central nervous system (CNS) after injury. Microglia accumulation at the damage site has been shown to be required for axon sprouting and for efficient regeneration. We characterized HmC1q as a novel chemotactic factor for leech microglial cell recruitment. In mammals, a C1q-binding protein (C1qBP alias gC1qR), which interacts with the globular head of C1q, has been reported to participate in C1q-mediated chemotaxis of blood immune cells. In this study, we evaluated the chemotactic activities of a recombinant form of HmC1q and its interaction with a newly characterized leech C1qBP that acts as its potential ligand. METHODS: Recombinant HmC1q (rHmC1q) was produced in the yeast Pichia pastoris. Chemotaxis assays were performed to investigate rHmC1q-dependent microglia migration. The involvement of a C1qBP-related molecule in this chemotaxis mechanism was assessed by flow cytometry and with affinity purification experiments. The cellular localization of C1qBP mRNA and protein in leech was investigated using immunohistochemistry and in situ hybridization techniques. RESULTS: rHmC1q-stimulated microglia migrate in a dose-dependent manner. This rHmC1q-induced chemotaxis was reduced when cells were preincubated with either anti-HmC1q or anti-human C1qBP antibodies. A C1qBP-related molecule was characterized in leech microglia. CONCLUSIONS: A previous study showed that recruitment of microglia is observed after HmC1q release at the cut end of axons. Here, we demonstrate that rHmC1q-dependent chemotaxis might be driven via a HmC1q-binding protein located on the microglial cell surface. Taken together, these results highlight the importance of the interaction between C1q and C1qBP in microglial activation leading to nerve repair in the medicinal leech.
Asunto(s)
Proteínas Portadoras/metabolismo , Quimiotaxis/fisiología , Complemento C1q/metabolismo , Hirudo medicinalis/citología , Microglía/fisiología , Sistema Nervioso/citología , Secuencia de Aminoácidos , Animales , Biotinilación , Proteínas Portadoras/biosíntesis , Proteínas Portadoras/genética , Quimiotaxis/efectos de los fármacos , Complemento C1q/genética , Complemento C1q/farmacología , Secuencia Conservada , Electroporación , Citometría de Flujo , Ganglios de Invertebrados/citología , Humanos , Microglía/efectos de los fármacos , ARN Mensajero/metabolismo , Proteínas Recombinantes/metabolismo , Alineación de Secuencia , Factores de Tiempo , Traumatismos del Sistema Nervioso/metabolismo , Traumatismos del Sistema Nervioso/patologíaRESUMEN
We study the role of nano-scale cues in controlling neuronal growth. We use photolithography to fabricate substrates with repeatable line-pattern ridges of nano-scale heights. We find that neuronal processes, which are of micron size, have strong interactions with ridges even as low as 10 nm. The interaction between the neuronal process and the ridge leads to a deflection of growth direction and a preferred alignment with the ridges. The interaction strength clearly depends on the ridges' height. For 25 nm ridges approximately half of the neuronal processes are modified, while at 100 nm the majority of neurites change their original growth direction post interaction. In addition, the effect on growth correlates with the incoming angle between the neuronal process and the ridge. We underline the adhesion as a key mechanism in directing neuronal growth. Our study highlights the sensitivity of growing neurites to nano-scale cues thus opens a new avenue of research for pre-designed neuronal growth and circuitry.
Asunto(s)
Hirudo medicinalis/citología , Neuronas/citología , Andamios del Tejido/química , Animales , Proliferación Celular , Células Cultivadas , Nanoestructuras/químicaRESUMEN
In contrast to mammals, the medicinal leech Hirudo medicinalis can completely repair its central nervous system (CNS) after injury. This invertebrate model offers unique opportunities to study the molecular and cellular basis of the CNS repair processes. When the leech CNS is injured, microglial cells migrate and accumulate at the site of lesion, a phenomenon known to be essential for the usual sprouting of injured axons. In the present study, we demonstrate that a new molecule, designated HmIL-16, having functional homologies with human interleukin-16 (IL-16), has chemotactic activity on leech microglial cells as observed using a gradient of human IL-16. Preincubation of microglial cells either with an anti-human IL-16 antibody or with anti-HmIL-16 antibody significantly reduced microglia migration induced by leech-conditioned medium. Functional homology was demonstrated further by the ability of HmIL-16 to promote human CD4+ T cell migration which was inhibited by antibody against human IL-16, an IL-16 antagonist peptide or soluble CD4. Immunohistochemistry of leech CNS indicates that HmIL-16 protein present in the neurons is rapidly transported and stored along the axonal processes to promote the recruitment of microglial cells to the injured axons. To our knowledge, this is the first identification of a functional interleukin-16 homologue in invertebrate CNS. The ability of HmIL-16 to recruit microglial cells to sites of CNS injury suggests a role for HmIL-16 in the crosstalk between neurons and microglia in the leech CNS repair.
Asunto(s)
Movimiento Celular/fisiología , Ganglios de Invertebrados/citología , Ganglios de Invertebrados/lesiones , Hirudo medicinalis/citología , Hirudo medicinalis/fisiología , Interleucina-16/fisiología , Microglía/fisiología , Homología de Secuencia de Aminoácido , Animales , Células Cultivadas , Modelos Animales de Enfermedad , Ganglios de Invertebrados/fisiología , Humanos , Interleucina-16/antagonistas & inhibidores , Microglía/citologíaRESUMEN
While glycosyltransferases are restrictively expressed in invertebrate model organisms, little is known of their glycan end products. One such restrictively expressed glycoepitope was localized to sensory and epithelial cells of leech and Caenorhabditis elegans using the Lan3-2 monoclonal antibody. A biological function for the neural Lan3-2 epitope was previously determined in the leech. Here we report on the chemical structure of this mannosidic epitope harvested from whole Hirudo medicinalis. Crude glycans were liberated from glycoproteins by hydrazinolysis. Re-N-acetylated glycans were subjected to immunoaffinity purification. The affinity-purified glycans were fractioned by size chromatography into oligosaccharides and polysaccharides. Lan3-2 oligosaccharide structure was characterized by gas chromatography of alditol acetates, methylation analysis, 500 MHz 1H NMR spectroscopy, matrix-assisted laser desorption/ionization mass spectrometry, and electrospray ionization tandem MS-MS of permethylated derivatives. The predominant components of the Lan3-2 oligosaccharide fraction were a series of linear beta-(1,4)-linked mannose polymers. The homologous expression of the Lan3-2 epitope in C. elegans will facilitate the exploration of its glycosylation pathway. Other invertebrates expressing the Lan3-2 epitope are Planaria dugesia, Capitella sp. I and Lumbriculus variegatus. The glycoepitope was not detected in the diploblastic animals Hydra littoralis and Aptaisia sp. or in deuterostomes.
Asunto(s)
Hirudo medicinalis/metabolismo , Manosa/metabolismo , Polisacáridos/metabolismo , Animales , Células Epiteliales/metabolismo , Hirudo medicinalis/química , Hirudo medicinalis/citología , Manosa/química , Oligosacáridos/química , Oligosacáridos/metabolismo , Células Receptoras Sensoriales/metabolismo , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodosRESUMEN
Glial cells can be activated by neurotransmitters via metabotropic, G protein-coupled receptors. We have studied the effects of 'global' G protein activation by GTP-gamma-S on the membrane potential, membrane conductance, intracellular Ca(2+) and Na(+) of the giant glial cell in isolated ganglia of the leech Hirudo medicinalis. Uncaging GTP-gamma-S (injected into a giant glial cell as caged compound) by moderate UV illumination hyperpolarized the membrane due to an increase in K+ conductance. Uncaging GTP-gamma-S also evoked rises in cytosolic Ca(2+) and Na+, both of which were suppressed after depleting the intracellular Ca(2+) stores with cyclopiazonic acid (20 micromol l(-1)). Uncaging inositol-trisphosphate evoked a transient rise in cytosolic Ca(2+) and Na+ but no change in membrane potential. Injection of the fast Ca(2+) chelator BAPTA or depletion of intracellular Ca(2+) stores did not suppress the membrane hyperpolarization induced by uncaging GTP-gamma-S. Our results suggest that global activation of G proteins in the leech giant glial cell results in a rise of Ca(2+)-independent membrane K+ conductance, a rise of cytosolic Ca(2+), due to release from intracellular stores, and a rise of cytosolic Na+, presumably due to increased Na+/Ca(2+) exchange.
Asunto(s)
Proteínas de Unión al GTP/metabolismo , Guanosina 5'-O-(3-Tiotrifosfato)/análogos & derivados , Hirudo medicinalis/metabolismo , Neuroglía/metabolismo , Animales , Calcio/metabolismo , Citosol/metabolismo , Guanosina 5'-O-(3-Tiotrifosfato)/química , Guanosina 5'-O-(3-Tiotrifosfato)/farmacología , Guanosina 5'-O-(3-Tiotrifosfato)/efectos de la radiación , Hirudo medicinalis/citología , Fosfatos de Inositol/metabolismo , Potenciales de la Membrana/efectos de los fármacos , Potasio/metabolismo , Transducción de Señal , Sodio/metabolismo , Rayos UltravioletaRESUMEN
Pump activity is a homeostatic mechanism that maintains ionic gradients. Here we examined whether the slow reduction in excitability induced by sodium-pump activity that has been seen in many neuronal types is also involved in neuronal coding. We took intracellular recordings from a spike-bursting sensory neuron in the leech Hirudo medicinalis in response to naturalistic tactile stimuli with different statistical distributions. We show that regulation of excitability by sodium pumps is necessary for the neuron to make different responses depending on the statistical context of the stimuli. In particular, sodium-pump activity allowed spike-burst sizes and rates to code not for stimulus values per se, but for their ratio with the standard deviation of the stimulus distribution. Modeling further showed that sodium pumps can be a general mechanism of adaptation to statistics on the time scale of 1 min. These results implicate the ubiquitous pump activity in the adaptation of neural codes to statistics.
Asunto(s)
Potenciales de Acción/fisiología , Adaptación Fisiológica/fisiología , Modelos Neurológicos , Neuronas Aferentes/fisiología , ATPasa Intercambiadora de Sodio-Potasio/fisiología , Potenciales de Acción/efectos de los fármacos , Animales , Apamina/farmacología , Hirudo medicinalis/citología , Neuronas Aferentes/efectos de los fármacos , Estimulación Física , Estrofantidina/farmacología , Factores de TiempoRESUMEN
The spatial and temporal patterns of action potential initiations were studied in a behaving leech preparation to determine the basis of increased firing that accompanies sensitization, a form of non-associative learning requiring the S-interneurons. Little is known at the network level about mechanisms of behavioral sensitization. The S-interneurons, one in each ganglion and linked by electrical synapses with both neighbors to form a chain, are interposed between sensory and motor neurons. In sensitized preparations the strength of shortening is related to S-cell firing, which itself is the result of impulses initiating in several S-cells. Because the S-cells, as independent initiation sites, all contribute to activity in the chain, it was hypothesized that during sensitization, increased multi-site activity increased the chain's firing rate. However, it was found that during sensitization, the single site with the largest initiation rate, the S-cell in the stimulated segment, suppressed initiations in adjacent ganglia. Experiments showed this was both because (1) it received the earliest, greatest input and (2) the delayed synaptic input to the adjacent S-cells coincided with the action potential refractory period. A compartmental model of the S-cell and its inputs showed that a simple, intrinsic mechanism of inexcitability after each action potential may account for suppression of impulse initiations. Thus, a non-synaptic competition between neurons alters synaptic integration in the chain. In one mode, inputs to different sites sum independently, whereas in another, synaptic input to a single site precisely specifies the overall pattern of activity.
Asunto(s)
Potenciales de Acción/fisiología , Hirudo medicinalis/fisiología , Interneuronas/fisiología , Aprendizaje/fisiología , Fenómenos Fisiológicos del Sistema Nervioso , Vías Nerviosas/fisiología , Animales , Estimulación Eléctrica , Sinapsis Eléctricas/fisiología , Ganglios de Invertebrados/citología , Ganglios de Invertebrados/fisiología , Hirudo medicinalis/citología , Interneuronas/citología , Red Nerviosa/citología , Red Nerviosa/fisiología , Vías Nerviosas/citología , Neuronas Aferentes/fisiología , Reflejo/fisiología , Periodo Refractario Electrofisiológico/fisiología , Transmisión Sináptica/fisiologíaRESUMEN
Damage to the leech or mammalian CNS increases nitric oxide (NO) production and causes accumulation of phagocytic microglial cells at the injury site. Opioids have been postulated to modulate various parameters of the immune response. Morphine and leech morphine-like substance are shown to release NO and suppress microglial activation. Regarding the known immuno-modulatory effects of selective mu and kappa ligands, we have assessed the effect of these agents on accumulation of microglia at the site of injury in leech CNS. Leech nerve cords were dissected, crushed with fine forceps and maintained in different concentrations of opiates in culture medium for 3 h and then fixed and double stained with Hoechst 33258 and monoclonal antibody to endothelial nitric oxide synthase (NOS). Morphine and naloxone (> or =10(-3) M) but not selective mu agonist, DAMGO [d-Ala2, N-Me-Phe-Gly5(ol)-enkephalin] and antagonist, CTAP [D-Phe-Cys-Tyr-D-Trp-Arg-Thr-Pen-Thr-NH2] inhibited the microglial accumulation. The effect of morphine was abrogated by pre-treatment with naloxone and also non-selective NOS inhibitor, l-NAME [N(omega)-nitro-l-arginine-methyl-ester; 10(-3) M] implying an NO-dependent and mu-mediated mechanism. These results are similar to properties of recently found mu-3 receptor in leech, which is sensitive to alkaloids but not peptides. Both selective kappa agonist, U50,488 [3,4-dichloro-N-methyl-N-(2-(1-pyrrolidinyl)cyclohexyl)-benzeneacetamide; > or =10(-3) M], and antagonist, nor-binaltorphimine (nor-BNI; > or =10(-3) M), inhibited the accumulation. The effect of nor-BNI was reversed by l-NAME. Immunohistochemistry showed decreased endothelial NOS expression in naloxone and U50,488-treated cords. Since, NO production at the injury site is hypothesized to act as a stop signal for microglias, opioid agents may exert their effect via changing of NO gradient along the cord resulting in disruption of accumulation. These results suggest an immuno-modulatory role for mu and kappa opioid receptors on injury-induced microglial accumulation which may be mediated via NO.
Asunto(s)
Analgésicos Opioides/farmacología , Hirudo medicinalis/metabolismo , Microglía/metabolismo , Sistema Nervioso/metabolismo , Óxido Nítrico/metabolismo , Traumatismos del Sistema Nervioso/metabolismo , Animales , Inhibidores Enzimáticos/farmacología , Gliosis/metabolismo , Gliosis/fisiopatología , Hirudo medicinalis/citología , Microglía/citología , NG-Nitroarginina Metil Éster/farmacología , Antagonistas de Narcóticos/farmacología , Sistema Nervioso/citología , Óxido Nítrico Sintasa/antagonistas & inhibidores , Óxido Nítrico Sintasa/metabolismo , Péptidos Opioides/metabolismo , Receptores Opioides kappa/agonistas , Receptores Opioides kappa/metabolismo , Receptores Opioides mu/agonistas , Receptores Opioides mu/metabolismo , Transducción de Señal/efectos de los fármacos , Transducción de Señal/fisiología , Traumatismos del Sistema Nervioso/fisiopatologíaRESUMEN
The clearance of synaptically released glutamate is one of the pivotal functions of glial cells. We have studied the role of 5-hydroxytryptamine (5-HT, 30 microM), a neurotransmitter and neurohormone in the leech central nervous system with a versatile action spectrum, on the efficacy of glial glutamate uptake. The activity of the glutamate uptake carrier in the giant glial cell in isolated ganglia of Hirudo medicinalis was monitored by measuring the membrane current and the change in the intracellular Na(+) concentration (Na(+) (i)) as induced by the glutamate carrier substrate D-aspartate (D-asp, 1 mM). 5-HT increased the D-asp-induced current (EC(50) at 5 microM) and rise in Na(+) (i), an effect which was mimicked by the membrane-permeable cyclic nucleotide analogue dibutyryl-cyclic AMP (db-cAMP). The adenylyl cyclase inhibitor SQ 22,536 and the protein kinase A antagonist Rp-cAMP inhibited the effect of 5-HT. Blocking the G protein in the giant glial cell by injecting GDP-beta-S suppressed the effect of 5-HT, but not the effect of db-cAMP, on the D-asp-induced current. Our results suggest that 5-HT enhances the glial uptake of glutamate via cAMP- and PKA-mediated pathway.
Asunto(s)
Sistema Nervioso Central/metabolismo , Ácido Glutámico/metabolismo , Hirudo medicinalis/metabolismo , Neuroglía/metabolismo , Serotonina/metabolismo , Inhibidores de Adenilato Ciclasa , Adenilil Ciclasas/metabolismo , Sistema de Transporte de Aminoácidos X-AG/efectos de los fármacos , Sistema de Transporte de Aminoácidos X-AG/metabolismo , Animales , Ácido Aspártico/metabolismo , Bucladesina/farmacología , Membrana Celular/efectos de los fármacos , Membrana Celular/metabolismo , Sistema Nervioso Central/citología , Sistema Nervioso Central/efectos de los fármacos , AMP Cíclico/metabolismo , Proteínas Quinasas Dependientes de AMP Cíclico/antagonistas & inhibidores , Proteínas Quinasas Dependientes de AMP Cíclico/metabolismo , Inhibidores Enzimáticos/farmacología , Ganglios de Invertebrados/citología , Ganglios de Invertebrados/efectos de los fármacos , Ganglios de Invertebrados/metabolismo , Hirudo medicinalis/citología , Potenciales de la Membrana/efectos de los fármacos , Potenciales de la Membrana/fisiología , Neuroglía/citología , Neuroglía/efectos de los fármacos , Neuronas/citología , Neuronas/metabolismo , Técnicas de Placa-Clamp , Receptores Acoplados a Proteínas G/efectos de los fármacos , Receptores Acoplados a Proteínas G/metabolismo , Serotonina/farmacología , Transducción de Señal/efectos de los fármacos , Transducción de Señal/fisiología , Sodio/metabolismo , Transmisión Sináptica/fisiología , Regulación hacia Arriba/efectos de los fármacos , Regulación hacia Arriba/fisiologíaRESUMEN
Two general trends in the evolution of the nervous system have been toward centralization of neuronal somata and cephalization of the central nervous system (CNS). These organizational trends are apparent in the nervous system of annelid worms, including leeches. To determine if the anterior brain of the leech serves functions similar to those of the brains of more complex organisms, including vertebrates, we ablated one of the two major regions of the cephalic brain--the subesophageal ganglion (SubEG). For anatomical reasons, ablations were performed in embryos, rather than in adults. At the end of embryonic development, we observed the leeches' spontaneous behaviour and their responses to moderate touch. We observed that, although the midbody ganglia of the leech CNS display a high degree of local autonomy, the cephalic brain provides generalized excitation to the rest of the CNS, is a source of selective inhibition that modulates behaviour, integrates sensory information from the head with signals from the rest of the body, and plays an important role in organizing at least some complicated whole-body behaviours. These roles of the leech cephalic brain are common features of brain function in many organisms, and our results are consistent with the hypothesis that they arose early in evolution and have been conserved in complex nervous systems.
Asunto(s)
Ganglios de Invertebrados/citología , Ganglios de Invertebrados/fisiología , Hirudo medicinalis/citología , Hirudo medicinalis/embriología , Neuronas/fisiología , Animales , Conducta Animal/fisiología , Ganglios de Invertebrados/anatomía & histología , Ganglios de Invertebrados/embriología , Hirudo medicinalis/fisiología , Actividad Motora/fisiología , Neuronas/citologíaRESUMEN
Extracellular matrix components play a key role during the angiogenic process for a correct development of blood vessels: fibroblasts are the main cell type involved in the regulation of ECM protein production. In this study we characterize H. medicinalis fibroblasts and demonstrate that they take part to the regulation of angiogenesis that occurs during wound healing process. Massive proliferation and phenotypic modification are two distinctive markers of fibroblast activation. These cells, that are usually responsible for collagen production and function as an energy reservoir, are recruited during wound healing to form a collagen scaffold through a direct mechanic action and through secretion of specific proteoglycans. In addition we show that the activity of fibroblasts is modulated by EGF, a growth factor involved in wound healing in vertebrates. The formation of bundles of collagen fibrils by fibroblasts is fundamental for the development and migration of new blood vessels in lesioned areas during wound repair: administration of lovastatin in explanted leeches affects fibroblasts, damages collagen "scaffold" and indirectly causes the reduction of neo-capillary formation.