Comparative effects of heat stress on photosynthesis and oxidative stress in Halophila ovalis and Thalassia hemprichii under different light conditions.

This study investigated the physiological responses of two tropical seagrass species, Halophila ovalis and Thalassia hemprichii, to heat stress under varying light conditions in a controlled 5-day experiment. The experimental design included four treatments: control, saturating light, heat stress under sub-saturating light, and heat stress under saturating light (combined stress). We assessed various parameters, including chlorophyll fluorescence, levels of reactive oxygen species (ROS), antioxidant enzyme activities, and growth rates. In H. ovalis, heat stress resulted in a significant reduction in the maximum quantum yield of photosystem II (Fv/Fm) regardless of the light condition. However, the effects of heat stress on the effective quantum yield of photosystem II (ɸPSII) were more pronounced under saturating light conditions. In T. hemprichii, saturating irradiance exacerbated the heat stress effects on Fv/Fm and ɸPSII, although the overall photoinhibition was less severe than in H. ovalis. Heat stress led to ROS accumulation in H. ovalis and reduced the activity of superoxide dismutase (SOD) and ascorbate peroxidase in the sub-saturating light condition. Conversely, T. hemprichii exhibited elevated SOD activity under saturating light. Heat stress suppressed the growth of both seagrass species, regardless of the light environment. The Biomarker Response Index indicated that H. ovalis displayed severe effects in the heat stress treatment under both light conditions, while T. hemprichii exhibited moderate effects in sub-saturating light and major effects in saturating light conditions. However, the Effect Addition Index revealed an antagonistic interaction between heat stress and high light in both seagrass species. This study underscores the intricate responses of seagrasses, emphasizing the importance of considering both local and global stressors when assessing their vulnerability.

Warming alters the network of physiological traits and their contribution to plant abundance.

The impact of global warming on plant abundance has been widely discussed, but it remains unclear how warming affects plant physiological traits, and how these traits contribute to the abundance of aquatic plants. We explored the adjustments in physiological traits of two common aquatic plant species (Potamogeton crispus L. and Elodea canadensis Michx.) and their links to plant abundance in three temperature treatments by determining twelve physiological traits and plant abundance over an 11-month period in outdoor mesocosms. This mesocosms facility has been running uninteruptedly for 16 years, rendering the plants a unique opportunity to adapt to the warming differences. We found that 1) warming reduced the starch storage in winter for P. crispus and in summer for E. canadensis while increased the nitrogenous substances (e.g., TN, FAA, and proline) in winter for P. crispus. 2) For E. canadensis, TC, starch, SC, and sucrose contents were higher in summer than in winter regardless of warming, while TC, SC, and sucrose contents were lower in summer for P. crispus. 3) Warming decreased the association strength between physiological traits and plant abundance for P. crispus but enhanced it for E. canadensis. 4) E. canadensis showed increased interaction strength among physiological traits under warming, indicating increased metabolic exertion in the response to warming, which contributed to the reduction in abundance. Trait interaction strength of P. crispus was reduced under warming, but with less impact on plant abundance compared with E. canadensis. Our study emphasizes that warming alters the network of plant physiological traits and their contribution to abundance and that different strengths of susceptibility to warming of the various plant species may alter the composition of plant communities in freshwater ecosystems.

Acidification alleviates the inhibition of hyposaline stress on physiological performance of tropical seagrass Thalassia hemprichii.

Since the Industrial Revolution, increasing atmospheric CO2 concentrations have had a substantial negative impact influence on coastal ecosystems because of direct effects including ocean acidification and indirect effects such as extreme rainfall events. Using a two-factor crossover indoor simulation experiment, this study examined the combined effects of acidification and hyposaline stress on Thalassia hemprichii. Seawater acidification increased the photosynthetic pigment content of T. hemprichii leaves and promoted seagrass growth rate. Hyposaline stress slowed down seagrass growth and had an impact on the osmotic potential and osmoregulatory substance content of seagrass leaves. Acidification and salinity reduction had significant interaction effects on the photosynthesis rate, photosynthetic pigment content, chlorophyll fluorescence parameters, and osmotic potential of T. hemprichii, but not on the growth rate. Overall, these findings have shown that the hyposaline stress inhibitory effect on the T. hemprichii physiological performance and growth may be reduced by acidification.

Alien emergent aquatic plants develop better ciprofloxacin tolerance and metabolic capacity than one native submerged species.

Antibiotic pollution and biological invasion pose significant risks to freshwater biodiversity and ecosystem health. However, few studies have compared the ecological adaptability and ciprofloxacin (CIPR) degradation potential between alien and native macrophytes. We examined growth, physiological response, and CIPR accumulation, translocation and metabolic abilities of two alien plants (Eichhornia crassipes and Myriophyllum aquaticum) and one native submerged species (Vallisneria natans) exposed to CIPR at 0, 1 and 10 mg/L. We found that E. crassipes and M. aquaticum's growth were unaffected by CIPR while V. natans was significantly hindered under the 10 mg/L treatment. CIPR significantly decreased the maximal quantum yield of PSII, actual quantum yield of PSII and relative electron transfer rate in E. crassipes and V. natans but didn't impact these photosynthetic characteristics in M. aquaticum. All the plants can accumulate, translocate and metabolize CIPR. M. aquaticum and E. crassipes in the 10 mg/L treatment group showed greater CIPR accumulation potential than V. natans indicated by higher CIPR contents in their roots. The oxidative cleavage of the piperazine ring acts as a key pathway for these aquatic plants to metabolize CIPR and the metabolites mainly distributed in plant roots. M. aquaticum and E. crassipes showed a higher production of CIPR metabolites compared to V. natans, with M. aquaticum exhibiting the strongest CIPR metabolic ability, as indicated by the most extensive structural breakdown of CIPR and the largest number of potential metabolic pathways. Taken together, alien species outperformed the native species in ecological adaptability, CIPR accumulation and metabolic capacity. These findings may shed light on the successful invasion mechanisms of alien aquatic species under antibiotic pressure and highlight the potential ecological impacts of alien species, particularly M. aquaticum. Additionally, the interaction of antibiotic contamination and invasion might further challenge the native submerged macrophytes and pose greater risks to freshwater ecosystems.

Response of a submerged macrophyte (Vallisneria natans) to water depth gradients and sediment nutrient concentrations.

Submerged plants constitute a vital component of shallow lake ecosystems, where water depth and sediment nitrogen­phosphorus content are two key factors influencing their growth. This study focuses on Vallisneria natans and investigates the morphological and physiological changes of V. natans under the interaction of three water depth gradients and two different sediment nutrient levels. It explores the mechanisms through which varying sediment nutrient conditions under different water depths affect the growth of V. natans. The results indicate that both independent and interactive effects of water depth and sediment nutrient status significantly impact the morphology, antioxidant enzyme activity, and photosynthetic pigment content of V. natans, with water depth having a greater influence. To adapt to increased water depth-induced light stress, V. natans responds morphologically by increasing leaf length, leaf width, and decreasing maximum root length. Physiologically, it enhances its antioxidant regulation capacity and photosynthetic efficiency by increasing antioxidant enzyme activity, root vitality, and photosynthetic pigment content to counter weak light stress. However, these adaptations are insufficient to cope with excessively deep waters (200 cm). Sediment nutrient levels primarily control the growth of V. natans by affecting its root system. When sediment nitrogen and phosphorus content is lower, V. natans exhibits greater total root volume and surface area to enhance nutrient absorption efficiency. Water depth not only directly influences the growth of submerged plants but may also impact the migration and transformation of phosphorus in sediments, further exacerbating its effects on the growth of these plants, thus accelerating the regime shift of shallow lakes. Therefore, this study reveals V. natans' response strategies to varying water depths and sediment nutrient levels, determining suitable water levels and sediment nutrient conditions for its growth. These research findings provide a scientific basis for water level management and ecological restoration of submerged aquatic plants in shallow lakes.

Effects of benthic fish and light regimes on water quality and the growth of Vallisneria natans with two sediment types.

In shal low eutrophic lakes, submersed macrophytes are essential for maintaining a clear water state and they are significantly affected by benthic fish disturbance, light availability, and sediment types. We conducted a mesocosm experiment with benthic fish (Misgurnus anguillicaudatus), two light regimes, and submerged macrophyte (Vallisneria natans) growing in two sediment types to investigate the ecological effects of benthic fish and light regimes on water quality and the growth of submersed macrophyte. Our findings indicated that the benthic fish increased the concentrations of total nitrogen, total phosphorus, and total dissolved phosphorus in the overlying water. The effects of benthic fish on ammonia-nitrogen (NH4+-N) and chlorophyll a (Chl-a) contents were related to light regimes. Fish disturbance indirectly promoted the growth of macrophytes growing in sand by increasing NH4+-N content in overlying water. However, the increasing Chl-a content stimulated by fish disturbance and high light regime reduced the growth of submersed macrophytes growing in clay due to shading. Macrophytes with different sediments had different strategies coping with light. Plants growing in sand responded to low light mainly by adjusting the leaf and root biomass allocation, whereas plants growing in clay responded to low light by physiologically adjusting the soluble carbohydrate content. The findings of this study might help restore lake vegetation to some degree, and using nutrient-poor sediment might be an appropriate method to avoid the detrimental effects of fish-mediated disturbances on the growth of submerged macrophytes.

The use of the aquatic plant Elodea canadensis to assess the effects of low-dose gamma irradiation.

Responses of cytogenetic and growth endpoints of the aquatic plant Elodea canadensis were studied using the plants collected from non-contaminated control areas of the Yenisei River and exposed to external γ-radiation for 11-13 days in the laboratory. Elodea canadensis was exposed to 0.5-25 mGy/day γ-radiation dose rates from a 137Cs source. The total number of aberrant cells and total root length were more sensitive elodea endpoints to γ-radiation than total shoot length and mitotic index of elodea. Radiation sensitivity of elodea can be compared with the sensitivity of such a Reference Plant as Wild Grass (1-10 mGy/day) recommended by the ICRP. Thus, the aquatic plant Elodea canadensis can be used as a radiation bioindicator.

Impacts of bisphenol A on growth and reproductive traits of submerged macrophyte Vallisneria natans.

Bisphenol A (BPA) is considered a contaminant of emerging concern and interferes with the normal activities of living organisms. The toxicity of BPA is evident in animals and terrestrial plants. However, the response of aquatic plants to low BPA concentrations is still unclear. In the present study, effects of varying BPA loadings (targeting at 0.01, 0.1, and 1 mg/L) on the growth and reproductive traits of the dioecious annual submerged macrophyte Vallisneria natans were assessed through a 5-month experiment. The results showed that BPA inhibited the elongation of V. natans leaves but resulted in an increase in leaf number and ramet number under the highest BPA loading treatment (targeting at 1 mg/L). In addition, detectable biochemical changes in the total carbon and soluble sugar contents were found, which both were significantly higher at the highest BPA loading treatment. However, the total biomass did not alter significantly after the BPA treatments, indicating that BPA did not induce direct toxic effects on the growth of V. natans. At the highest BPA loading treatment, female individuals of V. natans allocated less number for ramet than male ones, showing a clear sexual dimorphism. No significant differences between the five treatments were found for the flower or fruit traits, while the germination rate was significantly inhibited for the seeds collected from the highest BPA loading treatment. In conclusion, V. natans tolerated low concentrations of BPA by making a trade-off between ramet (leaf) number and leaf elongation, as well as modulating the total carbon and soluble sugar contents. However, serious consequence of decline in seed viability implied that the impact of BPA on plant reproduction were usually underestimated.

Response of submerged macrophytes and leaf biofilms to different concentrations of oxytetracycline and sulfadiazine.

Oxytetracycline and sulfadiazine were widely used and they entered the environment through various channels such as domestic sewage, medical wastewater and agricultural wastewater, causing significant ecological risk. To determine the effects of different antibiotic concentrations on submerged macrophytes, Vallisneria natans was exposed to solutions containing different concentrations of oxytetracycline and sulfadiazine (0.1 mg/L、1 mg/L、10 mg/L、50 mg/L). After 20-days exposure, we found that 10 mg/L groups had a significant effect on Vallisneria natans. Under high antibiotic concentrations, the growth of Vallisneria natans was inhibited, chloroplasts were deformed, the chlorophyll content was reduced, and antioxidant enzyme activities, such as superoxide dismutase and glutathione, were increased. There was no significant difference between the control group and groups with low antibiotic concentrations (≤1 mg/L). The N-acyl-l-homoserine lactone concentration tended to increase with increasing antibiotic concentrations. The presence of antibiotics also affected the microbial community structure of biofilms on the submerged macrophytes. For example, the higher the concentration of antibiotics, the higher the proportion of Proteobacteria. These results suggest that high concentrations of oxytetracycline and sulfadiazine can disrupt homeostasis, induce effective Vallisneria natans defense mechanisms and alter biofilms in aquatic ecosystems.

Role of phosphorus in Vallisneria natans and biofilm exposure to Pb2+ and Cd2+ stress.

Phosphorus (P) could improve the stress resistance and adaptability of submerged macrophytes. This study investigated the physiological and biochemical responses of plants exposed to different P and Pb, Cd concentrations. Alterations of protein synthesis, the DNA methylation (5-mC) level, and the microbial community of biofilm were also evaluated. Results indicated that lower P (0.5 mg·L-1) could promote plant growth and metal enrichment while mitigating the toxicity of metals. Higher P (5.0 mg·L-1) induced a degree of oxidative stress, as confirmed by increased activity of superoxide dismutase, peroxidase, and acid phosphatase, as well as increased malondialdehyde contents. While the variation of metallothionein synthesis and DNA methylation level of the plant was dependent on the level of P and metals in the water. These responses indicated potential mechanisms of P detoxification and intoxication. In addition, more abundant microbial communities were observed in biofilms exposed to P and metals. These findings provide theoretical support for the metal detoxification of P in submerged plants.

Comparative study on anatomical traits and gas exchange responses due to belowground hypoxic stress and thermal stress in three tropical seagrasses.

BACKGROUND: The ability to maintain sufficient oxygen levels in the belowground tissues and the rhizosphere is crucial for the growth and survival of seagrasses in habitats with highly reduced sediment. Such ability varies depending on plant anatomical features and environmental conditions. METHODS: In the present study, we compared anatomical structures of roots, rhizomes and leaves of the tropical intertidal seagrasses, Cymodocea rotundata, Thalassia hemprichii and Halophila ovalis, followed by an investigation of their gas exchange both in the belowground and aboveground tissues and photosynthetic electron transport rates (ETR) in response to experimental manipulations of O2 level (normoxia and root hypoxia) and temperature (30 °C and 40 °C). RESULTS: We found that C. rotundata and T. hemprichii displayed mostly comparable anatomical structures, whereas H. ovalis displayed various distinctive features, including leaf porosity, number and size of lacunae in roots and rhizomes and structure of radial O2 loss (ROL) barrier. H. ovalis also showed unique responses to root hypoxia and heat stress. Root hypoxia increased O2 release from belowground tissues and overall photosynthetic activity of H. ovalis but did not affect the other two seagrasses. More pronounced warming effects were detected in H. ovalis, measured as lower O2 release in the belowground tissues and overall photosynthetic capacity (O2 release and dissolved inorganic carbon uptake in the light and ETR). High temperature inhibited photosynthesis of C. rotundata and T. hemprichii but did not affect their O2 release in belowground tissues. Our data show that seagrasses inhabiting the same area respond differently to root hypoxia and temperature, possibly due to their differences in anatomical and physiological attributes. Halophila ovalis is highly dependent on photosynthesis and appears to be the most sensitive species with the highest tendency of O2 loss in hypoxic sediment. At the same time, its root oxidation capacity may be compromised under warming scenarios.

Large-Scale Green Liver System for Sustainable Purification of Aquacultural Wastewater: Construction and Case Study in a Semiarid Area of Brazil (Itacuruba, Pernambuco) Using the Naturally Occurring Cyanotoxin Microcystin as Efficiency Indicator.

The aquaculture industry in Brazil has grown immensely resulting in the production of inefficiently discarded wastewater, which causes adverse effects on the aquatic ecosystem. The efficient treatment of aquaculture wastewater is vital in reaching a sustainable and ecological way of fish farming. Bioremediation in the form of the Green Liver System employing macrophytes was considered as wastewater treatment for a tilapia farm, COOPVALE, in Itacuruba, Brazil, based on previously demonstrated success. A large-scale system was constructed, and the macrophytes Azolla caroliniana, Egeria densa, Myriophyllum aquaticum, and Eichhornia crassipes were selected for phytoremediation. As cyanobacterial blooms persisted in the eutrophic wastewater, two microcystin congeners (MC-LR and -RR) were used as indicator contaminants for system efficiency and monitored by liquid-chromatography-tandem-mass-spectrometry. Two trial studies were conducted to decide on the final macrophyte selection and layout of the Green Liver System. In the first trial, 58% MC-LR and 66% MC-RR were removed and up to 32% MC-LR and 100% MC-RR were removed in the second trial. Additional risks that were overcome included animals grazing on the macrophytes and tilapia were spilling over from the hatchery. The implementation of the Green Liver System significantly contributed to the bioremediation of contaminants from the fish farm.

Host and environmental determinants of microbial community structure in the marine phyllosphere.

Although seagrasses are economically and ecologically critical species, little is known about their blade surface microbial communities and how these communities relate to the plant host. To determine microbial community composition and diversity on seagrass blade surfaces and in the surrounding seawater,16S rRNA gene sequencing (iTag) was used for samples collected at five sites along a gradient of freshwater input in the northern Gulf of Mexico on three separate sampling dates. Additionally, seagrass surveys were performed and environmental parameters were measured to characterize host characteristics and the abiotic conditions at each site. Results showed that Thalassia testudinum (turtle grass) blades hosted unique microbial communities that were distinct in composition and diversity from the water column. Environmental conditions, including water depth, salinity, and temperature, influenced community structure as blade surface microbial communities varied among sites and sampling dates in correlation with changes in environmental parameters. Microbial community composition also correlated with seagrass host characteristics, including growth rates and blade nutrient composition. There is some evidence for a core community for T. testudinum as 21 microorganisms from five phyla (Cyanobacteria, Proteobacteria, Planctomycetes, Chloroflexi, and Bacteroidetes) were present in all blade surface samples. This study provides new insights and understanding of the processes that influence the structure of marine phyllosphere communities, how these microbial communities relate to their host, and their role as a part of the seagrass holobiont, which is an important contribution given the current decline of seagrass coverage worldwide.

Mimicry of emergent traits amplifies coastal restoration success.

Restoration is becoming a vital tool to counteract coastal ecosystem degradation. Modifying transplant designs of habitat-forming organisms from dispersed to clumped can amplify coastal restoration yields as it generates self-facilitation from emergent traits, i.e. traits not expressed by individuals or small clones, but that emerge in clumped individuals or large clones. Here, we advance restoration science by mimicking key emergent traits that locally suppress physical stress using biodegradable establishment structures. Experiments across (sub)tropical and temperate seagrass and salt marsh systems demonstrate greatly enhanced yields when individuals are transplanted within structures mimicking emergent traits that suppress waves or sediment mobility. Specifically, belowground mimics of dense root mats most facilitate seagrasses via sediment stabilization, while mimics of aboveground plant structures most facilitate marsh grasses by reducing stem movement. Mimicking key emergent traits may allow upscaling of restoration in many ecosystems that depend on self-facilitation for persistence, by constraining biological material requirements and implementation costs.

Responses of Hydrocharis dubia (Bl.) Backer and Trapa bispinosa roxb. to tetracycline exposure.

The presence of tetracycline is ubiquitous and has adverse effects on aquatic systems. A hydroponic experiment was conducted to investigate the ecological sensitivity of Hydrocharis dubia (Bl.) Backer and Trapa bispinosa Roxb. Exposed to different concentrations of tetracycline (0, 0.1, 1, 10, 30 and 50 mg/L) for one day (1D) and 14 days (14D). The results showed that after 1D of tetracycline exposure, the physiological indices of H. dubia had no remarkable change except for proline which was significantly stimulated under 0.1 mg/L tetracycline. For T. bispinosa, guaiacol peroxidase (POD), polyphenol oxidase (PPO) and ascorbate peroxidase (APX) activity and protein and proline content were notably promoted under different concentrations of tetracycline, but PPO activity was significantly decreased in 50 mg/L. After 14D, tetracycline caused no harm to the growth and protein content of H. dubia, but negatively influenced lipid peroxidation product and chlorophyll content in H. dubia under high tetracycline concentrations. Superoxide dismutase (SOD) and POD activity of H. dubia significantly increased at high tetracycline concentrations, while catalase (CAT) and PPO activity significantly decreased. APX activity in H. dubia increased with tetracycline concentrations at low tetracycline concentrations. For T. bispinosa, high concentrations of tetracycline application significantly inhibited its growth and the content of protein and chlorophyll. SOD, POD, CAT, and PPO activity of T. bispinosa were induced under different concentrations of tetracycline and no lipid peroxidation was observed. APX activity in T. bispinosa was significantly inhibited at high tetracycline concentrations. The results suggest that tetracycline can cause oxidative damage in H. dubia but harm the metabolism process of T. bispinosa without inducing oxidative damage. Overall, the sensitivity of T. bispinosa exposed to tetracycline exposure is higher than that of H. dubia.

Response of the submerged macrophytes Vallisneria natans to snails at different densities.

The study investigated the responses of the submerged macrophyte Vallisneria natans (V. natans) to snails (Bellamya aeruginosa) at different densities, with changes in physiological parameters, morphology, leaf-epiphytic bacteria community and water quality parameters examined. The changes of water quality parameters (pH, total nitrogen (TN), total phosphorus (TP) and total organic carbon (TOC)) indicated that snails secreted nutrients into water. Changes in morphological and physiological parameters (fresh weight, root length, shoot height, chlorophyll, malondialdehyde (MDA), activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD)) demonstrated that the presence of snails were beneficial to the growth of submerged macrophytes. Microbial diversity analyses indicated that snails could decrease microbial community richness and diversity. At medium densities (340 ind. m-2), an increase in snail density was beneficial to the growth of submerged macrophytes. The results of this study provide theoretical guidance and technical support for the maintenance and restoration of submerged macrophytes.

Tropical seagrass Halophila stipulacea shifts thermal tolerance during Mediterranean invasion.

Exotic species often face new environmental conditions that are different from those that they are adapted to. The tropical seagrass Halophila stipulacea is a Lessepsian migrant that colonized the Mediterranean Sea around 100 years ago, where at present the minimum seawater temperature is cooler than in its native range in the Red Sea. Here, we tested if the temperature range in which H. stipulacea can exist is conserved within the species or if the exotic populations have shifted their thermal breadth and optimum due to the cooler conditions in the Mediterranean. We did so by comparing the thermal niche (e.g. optimal temperatures, and upper and lower thermal limits) of native (Saudi Arabia in the Red Sea) and exotic (Greece and Cyprus in the Mediterranean Sea) populations of H. stipulacea. We exposed plants to 12 temperature treatments ranging from 8 to 40°C for 7 days. At the end of the incubation period, we measured survival, rhizome elongation, shoot recruitment, net population growth and metabolic rates. Upper and lower lethal thermal thresholds (indicated by 50% plant mortality) were conserved across populations, but minimum and optimal temperatures for growth and oxygen production were lower for Mediterranean populations than for the Red Sea one. The displacement of the thermal niche of exotic populations towards the colder Mediterranean Sea regime could have occurred within 175 clonal generations.

Species identity and diversity effects on invasion resistance of tropical freshwater plant communities.

Biotic resistance mediated by native plant diversity has long been hypothesized to reduce the success of invading plant species in terrestrial systems in temperate regions. However, still little is known about the mechanisms driving invasion patterns in other biomes or latitudes. We help to fill this gap by investigating how native plant community presence and diversity, and the presence of native phylogenetically closely related species to an invader, would affect invader Hydrilla verticillata establishment success in tropical freshwater submerged plant communities. The presence of a native community suppressed the growth of H. verticillata, but did not prevent its colonisation. Invader growth was negatively affected by native plant productivity, but independent of native species richness and phylogenetic relatedness to the invader. Native plant production was not related to native species richness in our study. We show that resistance in these tropical aquatic submerged plant communities is mainly driven by the presence and biomass of a native community independent of native species diversity. Our study illustrates that resistance provided by these tropical freshwater submerged plant communities to invasive species contrasts to resistance described for other ecosystems. This emphasizes the need to include understudied systems when predicting patterns of species invasiveness and ecosystem invasibility across biomes.

Ingenious floral structure drives explosive pollination in Hydrilla verticillata (Hydrocharitaceae).

In explosive pollination, many structures and mechanisms have evolved to achieve high-speed stamen movement. The male flower of the submerged plant Hydrilla verticillata is reported to be able to release pollen explosively some time after leaving the mother plant time, but the mechanism of stamen movement and the related functional structure in this species are unclear. In this study, we observed the male flower structure and pollen dispersal process of H. verticillata. We analysed the stamen movements during the pollen dispersal process and conducted several controlled experiments to study the process of storage and release of elastic potential energy in explosive pollination. When the male flower of H. verticillata is bound to the united bracts, the sepals accumulate elastic potential energy through the expansion of basal extensor cells. After the male flower is liberated from the mother plant, the stamens unfold rapidly with the sepals under adhesion and transfer the elastic potential energy to the filament in seconds. Once stamens unfold to a critical angle, at which the elasticity of the filament just exceeds the adhesion between sepals and anthers, the stamens automatically rebound and release pollen in milliseconds. These results reveal that Catapult-like stamens, spoon-shaped sepals and enclosed united bracts in the spathe together constitute the functional structure in rapid stamen movement of H. verticillata. They ensure that the pollen can be released on the water surface, and thus adapt successfully to the pollen-epihydrophilous pollination.

Response of submerged macrophytes and periphyton biofilm to water flow in eutrophic environment: Plant structural, physicochemical and microbial properties.

The integrated effects of water flow on submerged macrophytes (Vallisneria natans) and leaf biofilms were comprehensively investigated in eutrophic microcosm. Changes in aquatic environmental factors were analyzed and water flow was found to elevate eutrophic water quality, especially in terms of TP removal. The removal efficiency of TP reached 78.95% in flowing water, which was more than 10-fold higher than in static water. Water flow altered the morphological and physiological characteristics of plants, decreasing the cell wall thickness and rate of photosynthesis, while promoting the accumulation of soluble sugar and protein in leaves. The starch content also increased with water flow, and significantly larger starch granules were observed in chloroplast. Furthermore, oxidative damage was evidenced by the consistently higher content of malondialdehyde in flowing water. Superoxide dismutase (SOD), peroxidase (POD) and Catalase (CAT) were induced in plants exposed to water flow, as an antioxidant stress response. The results of 16S rRNA high-throughput sequencing analysis showed that the structure of the biofilm microbial community changed in response to water flow. These results expand our understanding of the effects of water flow on submerged macrophytes and periphyton biofilms in eutrophic environments.