RESUMEN
The period of forming of superficial vascular plexus during physiological retinal angiogenesis was shorter in C57Bl/6 mice. Experiments on the model of oxygen-induced retinopathy showed that avascular and vascularized zones in BALB/c mice on day 17 are smaller than in C57Bl/6 mice are by 5 and 1.5 times, respectively. The obtained results confirmed the importance of phenotype of retinal macrophages in the regulation of processes of both physiological and pathological retinal angiogenesis.
Asunto(s)
Retinopatía Hipertensiva/patología , Macrófagos/citología , Neovascularización Patológica/patología , Fenotipo , Retina/patología , Neovascularización Retiniana/patología , Animales , Fluoresceína-5-Isotiocianato/química , Retinopatía Hipertensiva/inducido químicamente , Retinopatía Hipertensiva/inmunología , Inmunohistoquímica , Inmunofenotipificación , Macrófagos/clasificación , Macrófagos/inmunología , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Neovascularización Patológica/inducido químicamente , Neovascularización Patológica/inmunología , Neovascularización Fisiológica/inmunología , Oxígeno/efectos adversos , Lectinas de Plantas/química , Retina/inmunología , Neovascularización Retiniana/inducido químicamente , Neovascularización Retiniana/inmunología , Especificidad de la Especie , Estreptavidina/químicaRESUMEN
To investigate the mechanism underlying AT1-AA-induced retinopathy in severe preeclampsia by measuring the positive rate and titer of AT1-AA in plasma from women with severe preeclampsia and normal pregnant women to see whether AT1-AA titer was correlated with the grade of retinopathy. A preeclampsia rat model was also established by intravenous injection of AT1-AA extracted from the plasma of patient suffering from severe preeclampsia. The results showed that the plasma titer and positive rate of AT1-AA were significantly higher in women with severe preeclampsia than normal pregnant women. The antibody titer in cases of severe preeclampsia was associated with the grade of retinopathy, and positively correlated with the level of TNF-α and VEGF. The animal experiment results showed that the modeled rats presented symptoms very similar to symptoms of human preeclampsia, including retinopathy. Ocular fundus examination showed retinal microvascular abnormalities, hemorrhaging and leakage in the severe preeclampsia. Morphological changes included edema, thickening of the INL and ONL, and pigment atrophy. TNF-α and VEGF levels were increased in the vitreous humor and retina of the model rats. Our studies results suggest that abnormal expression of AT1-AA could induce damage to retinal capillary endothelial cells and increase vascular permeability, resulting in retinopathy.
