RESUMEN
Although connections between the orbitofrontal cortex (OFC)-the seat of high cognitive functions-the lateral hypothalamus and the periaqueductal grey (PAG) have been recognized in the past, the precise targets of the descending fibres have not been identified. In the present study, viral tracer-transport experiments revealed neurons of the lateral (LO) and the ventrolateral (VLO) OFC (homologous to part of Area 13 in primates) to project to a circumscribed region in the ventrolateral hypothalamus, namely, the horizontally oriented, cylindrical parvalbumin- and Foxb1-expressing (parvafox) nucleus. The fine collaterals stem from coarse axons in the internal capsule and form excitatory synapses specifically with neurons of the parvafox nucleus, avoiding the rest of the hypothalamus. In its further caudal course, this contingent of LO/VLO-axons projects collaterals to the Su3- and the PV2 nuclei, which lie ventral to the aqueduct in the (PAG), where the terminals fields overlap those deriving from the parvafox nucleus itself. The targeting of the parvafox nucleus by the LO/VLO-projections, and the overlapping of their terminal fields within the PAG, suggest that the two cerebral sites interact closely. An involvement of this LO/VLO-driven circuit in the somatic manifestation of behavioural events is conceivable.
Asunto(s)
Área Hipotalámica Lateral/fisiología , Sustancia Gris Periacueductal/fisiología , Corteza Prefrontal/fisiología , Animales , Femenino , Factores de Transcripción Forkhead/genética , Factores de Transcripción Forkhead/metabolismo , Genes Reporteros , Área Hipotalámica Lateral/metabolismo , Área Hipotalámica Lateral/ultraestructura , Proteínas Luminiscentes/genética , Proteínas Luminiscentes/metabolismo , Masculino , Ratones Endogámicos C57BL , Ratones Transgénicos , Vías Nerviosas/fisiología , Técnicas de Trazados de Vías Neuroanatómicas/métodos , Parvalbúminas/genética , Parvalbúminas/metabolismo , Sustancia Gris Periacueductal/metabolismo , Sustancia Gris Periacueductal/ultraestructura , Corteza Prefrontal/metabolismo , Corteza Prefrontal/ultraestructura , Ratas Wistar , Proteínas Recombinantes de Fusión/metabolismoRESUMEN
Homeostasis describes the fundamental biological ability of individuals to maintain stable internal conditions in a changing environment. Homeostatic reactions include internal adjustments as well as behavioral responses. In vertebrates, behavioral responses are induced by the reward system. This system originates in the ventral tegmental area (VTA) and leads to increased dopamine levels in the forebrain whenever activated. A major inhibitor of VTA activity is the lateral habenula (LHb). This epithalamic structure is able to almost completely suppress dopamine release, either directly or via the rostromedial tegmental nucleus (RMTg), when rewarding expectations are not met. A major input to the LHb arises from the lateral hypothalamic area (LHA), an important regulator of the homeostatic system. Currently, little is known about the effects of the strong hypothalamic projection on the activity of LHb neurons. In the present study, we analyze neurotransmitters and cellular targets of the LHA-LHb projection in the rat. Therefore, anterograde tracing from the LHA was combined with the visualization of neurotransmitters in the LHb. These experiments revealed a mainly glutamatergic projection, probably exerting excitatory effects on the targeted LHb cells. These cellular targets were analyzed in a second step. Anterograde tracing from the LHA in combination with retrograde tracing from the VTA/RMTg region revealed that LHb neurons projecting to the VTA/RMTg region are densely targeted by the LHA projection. Visualization of synaptophysin at these contact sites indicates that the contact sites indeed are synapses. Taken together, the present study describes a strong mainly glutamatergic projection from the LHA that targets VTA/RMTg-projecting neurons in the LHb. These findings emphasize the potential role of the LHb as direct link between homeostatic areas and reward circuitries, which may be important for the control of homeostatic behaviors.
Asunto(s)
Ácido Glutámico/metabolismo , Habénula/citología , Área Hipotalámica Lateral/citología , Área Tegmental Ventral/citología , Animales , Habénula/ultraestructura , Área Hipotalámica Lateral/metabolismo , Área Hipotalámica Lateral/ultraestructura , Masculino , Vías Nerviosas/anatomía & histología , Vías Nerviosas/ultraestructura , Neuronas/metabolismo , Neuronas/ultraestructura , Ratas , Ratas Wistar , Área Tegmental Ventral/ultraestructuraRESUMEN
Melanin-concentrating hormone (MCH) is involved in the regulation of feeding behavior as well as in goal oriented behaviors, and MCH-containing neurons are distributed mainly in the lateral hypothalamic area (LHA). The anterior basomedial nucleus (BMA) and anterior cortical nucleus (CoA) of the amygdala form part of a circuit involved in processing olfactory, gustatory and visceral information, and the BMA-LHA and CoA-LHA pathways are suggested to be implicated in the control of feeding behavior. However, it is still unknown whether or not MCH-containing LHA neurons are under the direct influence of the BMA and CoA. Here the organization of projections from the BMA and CoA to MCH-containing LHA neurons was examined. Using a combined anterograde tracing with biotinylated dextranamine and immunohistochemistry for MCH, we first demonstrated that the distribution pattern of BMA fibers was almost similar to that of CoA fibers in the LHA, and a prominent overlapping distribution of these fibers and MCH-immunoreactive neurons existed in the ventral peripeduncular region of the LHA. We further revealed that asymmetrical synapses were made between these fibers and neurons. Using a combination of retrograde tract-tracing with cholera toxin B subunit and in situ hybridization for vesicular glutamate transporter (VGLUT) 2 mRNA, we finally showed that most of the LHA-projecting BMA and CoA neurons expressed VGLUT2 mRNA. These data suggest that the BMA and CoA of the amygdala may exert excitatory influence upon the MCH-containing LHA neurons for the regulation of feeding behavior.
Asunto(s)
Amígdala del Cerebelo/fisiología , Área Hipotalámica Lateral/fisiología , Hormonas Hipotalámicas/fisiología , Melaninas/fisiología , Neuronas/fisiología , Hormonas Hipofisarias/fisiología , Amígdala del Cerebelo/química , Amígdala del Cerebelo/ultraestructura , Animales , Área Hipotalámica Lateral/química , Área Hipotalámica Lateral/ultraestructura , Hormonas Hipotalámicas/análisis , Masculino , Melaninas/análisis , Red Nerviosa/química , Red Nerviosa/fisiología , Red Nerviosa/ultraestructura , Vías Nerviosas/química , Vías Nerviosas/fisiología , Vías Nerviosas/ultraestructura , Neuronas/química , Neuronas/ultraestructura , Hormonas Hipofisarias/análisis , Ratas , Ratas WistarRESUMEN
In the lateral hypothalamus, groups of functionally related cells tend to be widely scattered rather than confined to discrete, anatomically distinct units. However, by using parvalbumin (PV)-specific antibodies, a solitary, compact cord of PV-immunoreactive cells (the PV1-nucleus) has been identified in the ventrolateral tuberal hypothalamus in various species. Here we describe the topography, the chemo-, cyto-, and myeloarchitectonics, and the ultrastructure of this PV1-nucleus in rodents. The PV1-nucleus is located within the ventrolateral division of the medial forebrain bundle. In the horizontal plane, it has a length of 1 mm in mice and 2 mm in rats. PV-immunoreactive perikarya fall into two distinct size categories and number (~800 in rats and ~400 in mice). They are intermingled with PV-negative neurons and coarse axons of the medial forebrain bundle, some of which are PV-positive. Symmetric and asymmetric synapses, as well as PV-positive and PV-negative fiber endings, terminate on the perikarya of both PV-positive and PV-negative neurons. PV-positive neurons of the PV1-nucleus express glutamate, not γ-aminobutyric acid (GABA), the neurotransmitter that is usually associated with PV-containing nerve cells. Although we could not find evidence that PV1 neurons express either catecholamines or known neuropeptides, they sometimes are interspersed with the fibers and terminals of such cells. From its analogous topographical situation, the PV1-nucleus could correspond to the lateral tuberal nucleus in humans. We anticipate that the presence of the marker protein PV in the PV1-nucleus of the rodent hypothalamus will facilitate future studies relating to the connectivity, transcriptomics, and function of this entity.
Asunto(s)
Área Hipotalámica Lateral/anatomía & histología , Parvalbúminas/metabolismo , Animales , Anticuerpos/análisis , Calbindina 2 , Calbindinas , Recuento de Células , Circulación Cerebrovascular/fisiología , Femenino , Glutamato Descarboxilasa/metabolismo , Ácido Glutámico/metabolismo , Área Hipotalámica Lateral/citología , Área Hipotalámica Lateral/ultraestructura , Inmunohistoquímica , Péptidos y Proteínas de Señalización Intracelular/metabolismo , Masculino , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Microscopía Electrónica , Neuronas/ultraestructura , Neuropéptidos/metabolismo , Orexinas , Lectinas de Plantas , Ratas , Ratas Wistar , Receptores N-Acetilglucosamina , Proteína G de Unión al Calcio S100/metabolismo , Ácido gamma-Aminobutírico/metabolismoRESUMEN
BACKGROUND: Based on its distribution in the brain, ecto-nucleoside triphosphate diphosphohydrolase 3 (NTPDase3) may play a role in the hypothalamic regulation of homeostatic systems, including feeding, sleep-wake behavior and reproduction. To further characterize the morphological attributes of NTPDase3-immunoreactive (IR) hypothalamic structures in the rat brain, here we investigated: 1.) The cellular and subcellular localization of NTPDase3; 2.) The effects of 17beta-estradiol on the expression level of hypothalamic NTPDase3; and 3.) The effects of NTPDase inhibition in hypothalamic synaptosomal preparations. METHODS: Combined light- and electron microscopic analyses were carried out to characterize the cellular and subcellular localization of NTPDase3-immunoreactivity. The effects of estrogen on hypothalamic NTPDase3 expression was studied by western blot technique. Finally, the effects of NTPDase inhibition on mitochondrial respiration were investigated using a Clark-type oxygen electrode. RESULTS: Combined light- and electron microscopic analysis of immunostained hypothalamic slices revealed that NTPDase3-IR is linked to ribosomes and mitochondria, is predominantly present in excitatory axon terminals and in distinct segments of the perikaryal plasma membrane. Immunohistochemical labeling of NTPDase3 and glutamic acid decarboxylase (GAD) indicated that gamma-amino-butyric-acid- (GABA) ergic hypothalamic neurons do not express NTPDase3, further suggesting that in the hypothalamus, NTPDase3 is predominantly present in excitatory neurons. We also investigated whether estrogen influences the expression level of NTPDase3 in the ventrobasal and lateral hypothalamus. A single subcutaneous injection of estrogen differentially increased NTPDase3 expression in the medial and lateral parts of the hypothalamus, indicating that this enzyme likely plays region-specific roles in estrogen-dependent hypothalamic regulatory mechanisms. Determination of mitochondrial respiration rates with and without the inhibition of NTPDases confirmed the presence of NTPDases, including NTPDase3 in neuronal mitochondria and showed that blockade of mitochondrial NTPDase functions decreases state 3 mitochondrial respiration rate and total mitochondrial respiratory capacity. CONCLUSION: Altogether, these results suggest the possibility that NTPDases, among them NTPDase3, may play an estrogen-dependent modulatory role in the regulation of intracellular availability of ATP needed for excitatory neuronal functions including neurotransmission.
Asunto(s)
Área Hipotalámica Lateral/enzimología , Pirofosfatasas/metabolismo , Animales , Western Blotting , Estradiol/farmacología , Femenino , Expresión Génica/efectos de los fármacos , Glutamato Descarboxilasa/análisis , Glutamato Descarboxilasa/metabolismo , Homeostasis/fisiología , Área Hipotalámica Lateral/efectos de los fármacos , Área Hipotalámica Lateral/ultraestructura , Masculino , Mitocondrias/efectos de los fármacos , Mitocondrias/metabolismo , Mitocondrias/fisiología , Oxígeno/metabolismo , Pirofosfatasas/análisis , Pirofosfatasas/antagonistas & inhibidores , Ratas , Ratas Sprague-Dawley , Ribosomas/metabolismoRESUMEN
BACKGROUND: Peripherin, a type III neuronal intermediate filament, is widely expressed in neurons of the peripheral nervous system and in selected central nervous system hindbrain areas with projections towards peripheral structures, such as cranial nerves and spinal cord neurons. Peripherin appears to play a role in neurite elongation during development and axonal regeneration, but its exact function is not known. We noticed high peripherin expression in the posterior hypothalamus of mice, and decided to investigate further the exact location of expression and function of peripherin in the mouse posterior hypothalamus. RESULTS: In situ hybridization indicated expression of peripherin in neurons with a distribution reminiscent of the histaminergic neurons, with little signal in any other part of the forebrain. Immunocytochemical staining for histidine decarboxylase and peripherin revealed extensive colocalization, showing that peripherin is produced by histaminergic neurons in all parts of the tuberomammillary nucleus. We next used histamine immunostaining in peripherin knockout, overexpressing and wild type mice to study if altered peripherin expression affects these neurons, but could not detect any visible difference in the appearance of these neurons or their axons. Peripherin knockout mice and heterozygotic littermates were used for measurement of locomotor activity, feeding, drinking, and energy expenditure. Both genotypes displayed diurnal rhythms with all the parameters higher during the dark period. The respiratory quotient, an indicator of the type of substrate being utilized, also exhibited a significant diurnal rhythm in both genotypes. The diurnal patterns and the average values of all the recorded parameters for 24 h, daytime and night time were not significantly different between the genotypes, however. CONCLUSION: In conclusion, we have shown that peripherin is expressed in the tuberomammillary neurons of the mouse hypothalamus. Monitoring of locomotor activity, feeding, drinking, and energy expenditure in mice either lacking or overexpressing peripherin did not reveal any difference, so the significance of peripherin in these neurons remains to be determined. The complete overlap between histidine decarboxylase and peripherin, both the protein and its mRNA, renders peripherin a useful new marker for histaminergic neurons in the hypothalamus.
Asunto(s)
Área Hipotalámica Lateral/metabolismo , Proteínas de Filamentos Intermediarios/genética , Glicoproteínas de Membrana/genética , Proteínas del Tejido Nervioso/genética , Proteínas de Neurofilamentos/genética , Neuronas/metabolismo , Animales , Conducta Animal/fisiología , Biomarcadores/análisis , Biomarcadores/metabolismo , Mapeo Encefálico , Ritmo Circadiano/genética , Ingestión de Líquidos/genética , Metabolismo Energético/genética , Conducta Alimentaria/fisiología , Femenino , Genotipo , Histamina/metabolismo , Área Hipotalámica Lateral/ultraestructura , Inmunohistoquímica , Hibridación in Situ , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Actividad Motora/genética , Neuronas/ultraestructura , Periferinas , ARN Mensajero/análisis , ARN Mensajero/biosíntesis , ARN Mensajero/metabolismoRESUMEN
The synaptic organization between and among the insular cortex (IC) axons, central amygdaloid nucleus (ACe) axons and posterolateral hypothalamus (PLH) neurons was investigated in the rat using double anterograde tracing and anterograde tracing combined with postembedding immunogold analysis. After ipsilateral injections of biotinylated dextran amine (BDA) into the IC and Phaseolus vulgaris-leucoagglutinin (PHA-L) into the ACe, the conspicuous overlapping distribution of BDA-labeled axon terminals and PHA-L-labeled axon terminals was found in the PLH region just medial to the subthalamic nucleus ipsilateral to the injection sites. At the electron microscopic level, approximately two-thirds of the IC terminals made synapses with small-sized dendrites and the rest did with dendritic spines of the PLH neurons, whereas about 79%, 16% and 5% of the ACe terminals established synapses with small- to medium-sized dendrites, somata, and dendritic spines, respectively, of the PLH neurons. In addition, the IC axon terminals contained densely packed round clear vesicles and their synapses were of asymmetrical type. On the other hand, most of the ACe terminals contained not only pleomorphic clear vesicles but also dense-cored vesicles and their synapses were of symmetrical type although some ACe terminals contained densely packed round clear vesicles and formed asymmetrical synapses. Most of the postsynaptic elements received synaptic inputs from the IC or ACe terminals, and some of single postsynaptic elements received convergent synaptic inputs from both sets of terminals. Furthermore, almost all the ACe terminals were revealed to be immunoreactive for gamma-aminobutyric acid (GABA), by using the anterograde BDA tracing technique combined with immunohistochemistry for GABA. The present data suggest that single PLH neurons are under the excitatory influence of the IC and/or inhibitory influence of the ACe in the circuitry involved in the regulation of cardiovascular functions.
Asunto(s)
Amígdala del Cerebelo/fisiología , Corteza Cerebral/fisiología , Área Hipotalámica Lateral/ultraestructura , Vías Nerviosas/ultraestructura , Sinapsis/ultraestructura , Amígdala del Cerebelo/anatomía & histología , Animales , Biotina/análogos & derivados , Biotina/metabolismo , Mapeo Encefálico , Corteza Cerebral/anatomía & histología , Dextranos/metabolismo , Área Hipotalámica Lateral/metabolismo , Inmunohistoquímica/métodos , Masculino , Microscopía Electrónica de Transmisión , Vías Nerviosas/metabolismo , Fitohemaglutininas/metabolismo , Ratas , Ratas Wistar , Sinapsis/metabolismo , Ácido gamma-Aminobutírico/metabolismoRESUMEN
Galanin-like peptide (GALP) is produced in neurones in the hypothalamic arcuate nucleus and is implicated in the neural control of feeding behaviour. Previously, we have reported that GALP immunoreactive fibres were in direct contact with orexin/hypocretin immunoreactive neurones in the rat lateral hypothalamus using double-immunofluorescence. Centrally administered GALP is known to stimulate feeding behaviour. However, the target neurones of this action have not been clarified. The present study aimed to determine features of the GALP-mediated neuronal feeding pathway in rat. Accordingly, at the ultrastructural level, GALP-immunoreactive axon terminals were found to make synapses on orexin/hypocretin immunoreactive cell bodies and dendritic processes in the lateral hypothalamus. c-Fos immunoreactivity was expressed in orexin/hypocretin-immunoreactive neurones but not in melanin concentrating hormone-immunoreactive neurones in the lateral hypothalamus at 90 min after the application of GALP by i.c.v. infusion. Furthermore, to determine whether GALP regulates feeding behaviour via orexin/hypocretin neurones, the feeding behaviour of rats was studied following GALP i.c.v. injection with or without anti-orexin A and B immunoglobulin (IgG) pretreatment. The anti-orexin IgGs markedly inhibited GALP-induced hyperphagia. These results suggest that orexin/hypocretin-containing neurones in the lateral hypothalamus are targeted by GALP, and that GALP-induced hyperphagia is mediated via orexin/hypocretin neurones in the rat hypothalamus.
Asunto(s)
Conducta Alimentaria/fisiología , Péptido Similar a Galanina/fisiología , Área Hipotalámica Lateral/metabolismo , Péptidos y Proteínas de Señalización Intracelular/metabolismo , Neuronas/metabolismo , Neuropéptidos/metabolismo , Animales , Núcleo Arqueado del Hipotálamo/metabolismo , Núcleo Arqueado del Hipotálamo/ultraestructura , Péptido Similar a Galanina/administración & dosificación , Área Hipotalámica Lateral/ultraestructura , Hormonas Hipotalámicas/metabolismo , Inmunohistoquímica , Inyecciones Intraventriculares , Masculino , Melaninas/metabolismo , Vías Nerviosas/metabolismo , Neuronas/ultraestructura , Orexinas , Hormonas Hipofisarias/metabolismo , Ratas , Ratas Wistar , Estadísticas no Paramétricas , Sinapsis/metabolismo , Sinapsis/ultraestructura , Distribución TisularRESUMEN
After ipsilateral injections of cholera toxin B subunit (CTb) into the nucleus of the solitary tract (NST) and biotinylated dextran amine (BDA) into the insular cortex (IC) or into the central amygdaloid nucleus (ACe) in the rat, the prominent overlapping distribution of CTb-labeled neurons and BDA-labeled axon terminals was found in the posterolateral hypothalamus (PLH) region just medial to the subthalamic nucleus ipsilateral to the injection sites. At the electron microscopic level, the IC terminals formed asymmetrical synaptic contacts with dendrites and dendritic spines of the NST-projecting PLH neurons, whereas the ACe terminals formed symmetrical synaptic contacts with somata and dendrites of the NST-projecting PLH neurons. The present data suggest that output signals from the IC and ACe may exert excitatory and inhibitory influences, respectively, upon the PLH neurons that project to the NST for regulating cardiovascular functions.
Asunto(s)
Amígdala del Cerebelo/fisiología , Corteza Cerebral/fisiología , Área Hipotalámica Lateral/fisiología , Núcleo Solitario/fisiología , Transmisión Sináptica/fisiología , Amígdala del Cerebelo/ultraestructura , Animales , Biotina/análogos & derivados , Corteza Cerebral/ultraestructura , Toxina del Cólera , Dextranos , Colorantes Fluorescentes , Área Hipotalámica Lateral/citología , Área Hipotalámica Lateral/ultraestructura , Masculino , Microscopía Electrónica , Fibras Nerviosas/fisiología , Fibras Nerviosas/ultraestructura , Neuronas/fisiología , Neuronas/ultraestructura , Terminales Presinápticos/fisiología , Terminales Presinápticos/ultraestructura , Ratas , Ratas WistarRESUMEN
The perifornical-lateral hypothalamic area (PF-LHA) has been implicated in the regulation of behavioural arousal. The PF-LHA contains several cell types including neurones expressing the peptides, hypocretin (HCRT; also called orexin) and melanin-concentrating hormone (MCH). Evidence suggests that most of the PF-LHA neurones, including HCRT neurones, are active during waking and quiescent during non-rapid eye movement (non-NREM) sleep. The PF-LHA contains local GABAergic interneurones and also receives GABAergic inputs from sleep-promoting regions in the preoptic area of the hypothalamus. We hypothesized that increased GABA-mediated inhibition within PF-LHA contributes to the suppression of neuronal activity during non-REM sleep. EEG and EMG activity of rats were monitored for 2 h during microdialytic delivery of artificial cerebrospinal fluid (aCSF) or bicuculline, a GABAA receptor antagonist, into the PF-LHA in spontaneously sleeping rats during the lights-on period. At the end of aCSF or bicuculline perfusion, rats were killed and c-Fos immunoreactivity (Fos-IR) in HCRT, MCH and other PF-LHA neurones was quantified. In response to bicuculline perfusion into the PF-LHA, rats exhibited a dose-dependent decrease in non-REM and REM sleep time and an increase in time awake. The number of HCRT, MCH and non-HCRT/non-MCH neurones exhibiting Fos-IR adjacent to the microdialysis probe also increased dose-dependently in response to bicuculline. However, significantly fewer MCH neurones exhibited Fos-IR in response to bicuculline as compared to HCRT and other PF-LHA neurones. These results support the hypothesis that PF-LHA neurones, including HCRT neurones, are subject to increased endogenous GABAergic inhibition during sleep. In contrast, MCH neurones appear to be subject to weaker GABAergic control during sleep.
Asunto(s)
Hormonas Hipotalámicas/fisiología , Péptidos y Proteínas de Señalización Intracelular/fisiología , Melaninas/fisiología , Neuronas/fisiología , Neuropéptidos/fisiología , Hormonas Hipofisarias/fisiología , Fases del Sueño/fisiología , Ácido gamma-Aminobutírico/fisiología , Animales , Bicuculina/farmacología , Ritmo Circadiano , Relación Dosis-Respuesta a Droga , Genes fos/fisiología , Área Hipotalámica Lateral/fisiología , Área Hipotalámica Lateral/ultraestructura , Masculino , Neuronas/efectos de los fármacos , Neuronas/ultraestructura , Orexinas , Ratas , Ratas Sprague-DawleyRESUMEN
The orexins (also known as hypocretins) are peptide neurotransmitters made by hypothalamic neurons that are thought to play an important role in regulating wake-sleep states. One terminal area for orexin neurons is the tuberomammillary nucleus, a histaminergic cell group that is wake-active, but the relationship of the orexinergic terminals to the tuberomammillary neurons has not been examined in detail. We studied the ultrastructure of orexin A-immunoreactive axons and terminals in the tuberomammillary nucleus using pre- and post-embedding electron microscopic protocols. We confirmed an abundant projection of orexin-immunoreactive boutons to both dorsal and ventral divisions of the tuberomammillary nucleus. These terminals made asymmetric synaptic contacts with proximal and intermediate dendrites of tuberomammillary neurons. They contained small, clear synaptic vesicles and up to 30-40 dense core vesicles were seen per terminal in a single section. Both pre- and post-embedding immunostaining revealed that orexin immunoreactivity was localized to the dense core vesicles, which were always at a distance from the synaptic specialization. We also found glutamate immunoreactivity in the small synaptic vesicles which were at the active zone of the synapses of many of the same terminals. Orexinergic afferents to the tuberomammillary neurons contain separate populations of orexinergic and glutamatergic vesicles, suggesting that the release of these neurotransmitters may be differentially regulated.
Asunto(s)
Vías Aferentes/metabolismo , Proteínas Portadoras/metabolismo , Ácido Glutámico/metabolismo , Área Hipotalámica Lateral/metabolismo , Péptidos y Proteínas de Señalización Intracelular , Neuropéptidos/metabolismo , Terminales Presinápticos/metabolismo , Adenosina Desaminasa/metabolismo , Vías Aferentes/ultraestructura , Animales , Histamina/metabolismo , Área Hipotalámica Lateral/ultraestructura , Inmunohistoquímica , Masculino , Microscopía Electrónica , Orexinas , Terminales Presinápticos/ultraestructura , Ratas , Ratas Sprague-Dawley , Membranas Sinápticas/metabolismo , Membranas Sinápticas/ultraestructura , Transmisión Sináptica/fisiología , Vesículas Sinápticas/metabolismo , Vesículas Sinápticas/ultraestructuraRESUMEN
Leptin regulates the release of histamine in the hypothalamus, however, the histaminergic neurons contain few leptin receptors. To reveal that alpha-melanocyte stimulating hormone (alpha-MSH)-containing neurons of the arcuate nucleus may mediate the effects of leptin to the histaminergic neurons, we studied the putative innervation of histamine-immunoreactive (IR) neurons by alpha-MSH-containing axons using double-labeling immunocytochemistry. In order to analyze the relationship of alpha-MSH- and histamine-IR elements, we also generated an antiserum against histamine that is compatible with acrolein-based fixatives commonly used for immuno-electron microscopic studies. The apposition of alpha-MSH-IR axons to histaminergic neurons was observed in all five subnuclei of the tuberomammillary nucleus. Both axo-somatic and axo-dendritic contacts were found. At the ultrastructural level, silver-intensified colloidal gold particles identified the histaminergic neurons, whose ultrastructure was well preserved after fixation with acrolein demonstrating that the new antiserum is a useful tool for the ultrastructural examination of the histaminergic system. The histamine-IR cells received synaptic inputs from alpha-MSH-IR axon terminals visualized by diaminobenzidine. These data indicate that alpha-MSH-synthesizing neurons innervate histaminergic neurons in the tuberomammillary nucleus and may relay the hormonal influence of leptin to the histaminergic system.
Asunto(s)
Axones/metabolismo , Conducta Alimentaria/fisiología , Histamina/metabolismo , Área Hipotalámica Lateral/fisiología , alfa-MSH/metabolismo , Acroleína , Animales , Axones/ultraestructura , Histamina/inmunología , Área Hipotalámica Lateral/ultraestructura , Sueros Inmunes/inmunología , Immunoblotting , Técnicas para Inmunoenzimas/métodos , Inmunohistoquímica , Leptina/metabolismo , Microscopía Electrónica , Neuronas/metabolismo , Neuronas/ultraestructura , Albúmina Sérica BovinaRESUMEN
Argyrophilic grain disease (AgD) is a late-onset dementia morphologically characterized by abundant neuropil grains (ArGs). ArGs are mainly found in the CA1 subfield of the cornu ammonis, entorhinal and transentorhinal cortices, the amygdala and the hypothalamic lateral tuberal nuclei. We have recently shown that abnormally phosphosphorylated tau protein is the main protein constituent of ArGs and that tau is hyperphosphorylated in up to 80p.100 of nerve cels in areas rich in ArGs. We could demonstrate that at least a subset of grains are formed within dendrites and dendritic side-branches of neurons containing hyperphosphylated tau. Morphology of dendrites containing grains suggests that a process of progressive dendritic shrinkage is taking place in neurons bearing ArGs. Furthermore it became apparent that the presence of ArGs is not necessarily associated with a cognitive decline. Our studies on AgD cases with and without dementia suggest that AgD is a progressive neurodegenerative disorder with early subclinical lesions in anterior part of the hippocampal formation. At later stages involvement of more caudal parts of the hippocampal formation generally results in a cognitive decline. Thus, one possible explanation for the dementia observed in some subjects with AgD is that there is a more widepread loss of postsynaptic structures, including synaptic contacts, throughout the hippocampus-entorhinal/parahippocampal complex and the amygdaloid nuclei. Most of the reported AgD cases are associated with neurofibrillary lesions (e.g. neurofibrillary tangles) which are also typical of Alzheimer's disease (AD). However, neurofibrillary changes do not exceed early (entorhinal and limbic) Braak stages which generally are not associated with a cognitive decline. Additional neuropathological features of AgD include oligodendroglial tau filamentous inclusions ( coiled bodies ), ballooned neurons and astrocytic tau pathology. The clinical features of AgD are poorly understood. However, preliminary data from retrospective studies suggest that in AgD behavioural disturbances will precede memory failure and memory decline. Furthermore, it has been shown that the ApoEe4 allele does not constitute a risk factor for the development of AgD. In conclusion it seems very likely that AgD is a distinct dementing disorder of old age that has to be distinguished from other tauopathies, e.g. AD, by both morphological and genetic criteria.
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Gránulos Citoplasmáticos/ultraestructura , Demencia/patología , Neurópilo/ultraestructura , Factores de Edad , Anciano , Alelos , Enfermedad de Alzheimer/metabolismo , Enfermedad de Alzheimer/patología , Amígdala del Cerebelo/química , Amígdala del Cerebelo/ultraestructura , Apolipoproteínas E/genética , Astrocitos/química , Astrocitos/ultraestructura , Trastornos del Conocimiento/etiología , Cuerpos Enrollados/química , Cuerpos Enrollados/ultraestructura , Gránulos Citoplasmáticos/química , Demencia/epidemiología , Demencia/genética , Demencia/metabolismo , Dendritas/química , Dendritas/ultraestructura , Corteza Entorrinal/química , Corteza Entorrinal/ultraestructura , Hipocampo/química , Hipocampo/ultraestructura , Humanos , Área Hipotalámica Lateral/química , Área Hipotalámica Lateral/ultraestructura , Trastornos de la Memoria/etiología , Proteínas del Tejido Nervioso/análisis , Enfermedades Neurodegenerativas/clasificación , Neuronas/química , Neuronas/ultraestructura , Neurópilo/química , Oligodendroglía/química , Oligodendroglía/ultraestructura , Fosforilación , Procesamiento Proteico-Postraduccional , Factores de Riesgo , Tinción con Nitrato de Plata , Proteínas tau/análisisRESUMEN
Orexins (orexin A and B) are recently identified neuropeptides implicated in the regulation of vigilance states and energy homeostasis. We have shown here the physiological significance of histaminergic neurons in the orexin-induced arousal responses. Immunohistochemical and electron microscopic techniques revealed direct synaptic interaction between orexin-immunoreactive nerve terminals and histidine decarboxylase-immunoreactive neurons in the TMN. Electrophysiological study revealed that orexins dose-dependently activate histaminergic neurons, which were freshly isolated from rats TMN region. To further evaluate, we examined the effect of pyrilamine, an H(1) receptor antagonist, on orexin-induced arousal response in rats. Simultaneously recordings of electroencephalograph and electromyograph showed that intracerebroventricular infusion of orexin A significantly increased the awake state in the light phase. Central application of pyrilamine significantly inhibited this response. These results strongly suggest that activation of histaminergic neurons by orexins might be important for modulation of the arousal.
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Proteínas Portadoras/fisiología , Histamina/fisiología , Péptidos y Proteínas de Señalización Intracelular , Neuronas/fisiología , Neuropéptidos/fisiología , Receptores de Neuropéptido/fisiología , Animales , Nivel de Alerta/efectos de los fármacos , Nivel de Alerta/fisiología , Proteínas Portadoras/genética , Proteínas Portadoras/farmacología , Antagonistas de los Receptores Histamínicos H1/farmacología , Histidina Descarboxilasa/metabolismo , Área Hipotalámica Lateral/efectos de los fármacos , Área Hipotalámica Lateral/fisiología , Área Hipotalámica Lateral/ultraestructura , Masculino , Microscopía Electrónica , Neuronas/efectos de los fármacos , Neuronas/ultraestructura , Neuropéptidos/genética , Neuropéptidos/farmacología , Receptores de Orexina , Orexinas , Pirilamina/farmacología , Ratas , Ratas Wistar , Receptores Acoplados a Proteínas GRESUMEN
The lateral preoptic and lateral hypothalamic regions contain the majority of the cell groups embedded in the fibre trajectories of the medial forebrain bundle on its course through the hypothalamus. Recent studies have extended considerably the parcellation of the lateral hypothalamic region, and therefore, the need to emphasize new insights into the anatomical organisation of projections from the neurons of the lateral hypothalamic region. In the present study we describe the anatomical organisation of efferent projections from the lateral preoptic and lateral hypothalamic regions to the hypothalamic paraventricular nucleus (PVN) on the basis of retrograde- and anterograde-tracing techniques. Iontophoretic injections of the retrograde tracer, cholera toxin subunit B, into the PVN revealed that most hypothalamic nuclei project to the PVN. Within the lateral hypothalamic region, retrogradely labelled cells were concentrated in the intermediate hypothalamic area, the lateral hypothalamic area, and the perifornical nucleus, whereas fewer retrogradely labelled cells were found in the lateral preoptic area. To determine the distribution of terminating fibres in subnuclei of the heterogeneous PVN, iontophoretic injections of the anterograde tracer Phaseolus vulgaris-leucoagglutinin were delivered into distinct areas of the lateral hypothalamic region. Neurons of the intermediate hypothalamic area projected mainly to the PVN subnuclei, which contained parvicellular neuroendocrine cells. In contrast, neurons of the rostral and tuberal parts of the lateral hypothalamic area and the perifornical nucleus projected to the PVN subnuclei, which contained parvicellular neurons that send descending projections to preganglionic cell groups in the medulla and spinal cord. The perifornical nucleus was the only area within the lateral hypothalamic region that consistently innervated magnocellular perikarya of the PVN. Finally, all areas of the lateral hypothalamic region contributed substantially to fibres terminating in the perinuclear shell of the PVN. These results demonstrate that anatomically distinct areas of the lateral hypothalamic region have distinct projections to subnuclei of the PVN and further substantiate the view that the lateral hypothalamic region as well as the PVN constitute anatomically and functionally heterogeneous structures.
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Área Hipotalámica Lateral/fisiología , Núcleo Hipotalámico Paraventricular/fisiología , Área Preóptica/fisiología , Animales , Tronco Encefálico/fisiología , Tronco Encefálico/ultraestructura , Toxina del Cólera , Vías Eferentes/fisiología , Vías Eferentes/ultraestructura , Área Hipotalámica Lateral/ultraestructura , Inmunohistoquímica , Masculino , Haz Prosencefálico Medial/fisiología , Haz Prosencefálico Medial/ultraestructura , Núcleo Hipotalámico Paraventricular/ultraestructura , Fitohemaglutininas , Área Preóptica/ultraestructura , Ratas , Ratas Wistar , Médula Espinal/fisiología , Médula Espinal/ultraestructuraRESUMEN
Reciprocal connections between lateral septal neurons and neurons in the lateral hypothalamus/lateral preoptic area were studied in the rat. The anterograde tracer Phaseolus vulgaris leucoagglutinin (PHA-L) and the retrograde tracer Fluoro-Gold (FG) were simultaneously injected into the lateral septum. After double-immunocytochemistry, PHA-L-labeled terminals were found in synaptic contact with dendrites of retrogradely FG-labeled neurons in the lateral hypothalamic/lateral preoptic area.
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Hipocampo/fisiología , Área Hipotalámica Lateral/fisiología , Neuronas/fisiología , Área Preóptica/fisiología , Estilbamidinas , Vías Aferentes/citología , Vías Aferentes/fisiología , Animales , Transporte Axonal , Axones/fisiología , Axones/ultraestructura , Dendritas/fisiología , Dendritas/ultraestructura , Colorantes Fluorescentes , Hipocampo/citología , Hipocampo/ultraestructura , Área Hipotalámica Lateral/citología , Área Hipotalámica Lateral/ultraestructura , Inmunohistoquímica , Microscopía Electrónica , Neuronas/citología , Neuronas/ultraestructura , Fitohemaglutininas , Área Preóptica/citología , Área Preóptica/ultraestructura , Ratas , Ratas Sprague-Dawley , Sinapsis/fisiología , Sinapsis/ultraestructuraRESUMEN
A population of neurons immunoreactive to an antiserum (AS) raised against ovine prolactin (LHPLI neurons) was previously described in the rat perifornical areas and lateral hypothalamus. In the present paper, we demonstrate by complementary immunocytochemical studies using AS to various biologically active peptides or neurotransmitters that these neurons are also detected by AS to bradykinin and to dynorphin B. Electron microscope examination shows that the LHPLI neurons are peptidergic neurons synthesizing apparently only one type of secretory granules. Numerous synapses on their perikarya and processes reflect the complexity of their relationships with other neuron populations, which have yet to be mapped and elucidated.
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Área Hipotalámica Lateral/química , Neuronas/química , Prolactina/análisis , Animales , Colchicina , Área Hipotalámica Lateral/ultraestructura , Inmunohistoquímica , Masculino , Neuronas/ultraestructura , Ratas , Ratas Sprague-DawleyRESUMEN
The organization of GABAergic elements in the histaminergic tuberomammillary nucleus has been examined by using antibodies against gamma-aminobutyric acid (GABA) and light and electron microscopy. Most neuronal perikarya of the ventral subgroup of the tuberomammillary nucleus were GABA immunoreactive (GABA-i). The morphology of the GABA-i perikarya was similar to the morphology of histaminergic perikarya described by Hayashi et al. ('84: J. Comp. Neurol. 229: 223-241) and Wouterlood et al. ('86: J. Comp. Neurol. 252:227-243). The GABA-i perikarya were contacted by relatively few terminals. The mean bouton covering ratio of GABA-i perikarya was 6.1%, whereas the mean bouton covering ratio for GABA-i dendrites in the tuberomammillary nucleus was 31%. Some of the presynaptic terminals were GABA-i. In addition, GABA-i perikarya and dendrites formed close contacts that never presented synaptic specializations. These results suggest that neurons of the histaminergic tuberomammillary nucleus contain the neurotransmitter GABA. Furthermore, GABA may act as a modulator of cellular processes within the tuberomammillary nucleus.
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Núcleo Celular/ultraestructura , Área Hipotalámica Lateral/ultraestructura , Ácido gamma-Aminobutírico/análisis , Animales , Dendritas/ultraestructura , Área Hipotalámica Lateral/citología , Masculino , Microscopía Inmunoelectrónica/métodos , Ratas , Ratas EndogámicasRESUMEN
Golgi examination of neurons of self-stimulation areas of the lateral hypothalamus and substantia nigra-ventral tegmental areas of adult Wistar rats that had experienced self-stimulation for 10 days revealed a significantly higher number of dendritic branching points in the two self-stimulation areas, and also in the hippocampus (CA3 pyramidal neurons) than in inexperienced rats.
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Dendritas/ultraestructura , Hipocampo/ultraestructura , Autoestimulación/fisiología , Sustancia Negra/ultraestructura , Animales , Electrodos Implantados , Hipocampo/fisiología , Área Hipotalámica Lateral/ultraestructura , Masculino , Ratas , Ratas Endogámicas , Sustancia Negra/fisiología , Tegmento Mesencefálico/fisiología , Tegmento Mesencefálico/ultraestructuraRESUMEN
The tracer Phaseolus vulgaris-leucoagglutinin (PHA-L) was injected into the lateral septum of the rat at different rostrocaudal locations to study the efferent septal projections to the anterior hypothalamus. For spatial correlation of these septofugal elements with the vasopressinergic system a dual immunocytochemical technique was used (i) to demonstrate nerve fibers and their corresponding bouton-like structures labeled with the tracer, and (ii) to identify vasopressin in the same section. The hypothalamic paraventricular and supraoptic nuclei, the accessory hypothalamic magnocellular system, and the suprachiasmatic nucleus are recipients of PHA-L-labeled fibers from all parts of the lateral septum. Close appositions between (i) these axons and their varicosities, and (ii) vasopressin-immunoreactive perikarya and their processes, putatively indicating functional interrelationships, were observed in all these nuclear areas, especially in their neuropil formations.