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1.
Trop Doct ; 54(3): 245-247, 2024 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-38562099

RESUMEN

Typhoid ileal perforation (TIP) is a common surgical emergency in low-middle income countries (LMICs). Its high surgical morbidity and mortality is due to its often late presentation or diagnosis, the patient's malnutrition, severe peritoneal contamination and unavailability of intensive care in most peripheral hospitals. This prompted the philosophy of minimizing the crisis by avoiding any repair or anastomosis, limiting the surgery in these physiologically compromised patients and performing only a temporary defunctioning ileostomy (DI) which could then be closed 10-12 weeks later.


Asunto(s)
Ileostomía , Perforación Intestinal , Fiebre Tifoidea , Humanos , Perforación Intestinal/cirugía , Perforación Intestinal/etiología , Fiebre Tifoidea/complicaciones , Enfermedades del Íleon/cirugía , Enfermedades del Íleon/etiología , Enfermedades del Íleon/microbiología , Íleon/cirugía
2.
Am Surg ; 90(6): 1744-1747, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38225921

RESUMEN

Histoplasma capsulatum is a dimorphic fungi endemic to the Ohio and Mississippi River valleys. Immunocompetent persons who become infected are generally asymptomatic or present with mild symptoms. Symptomatic disease is seen primarily in immunocompromised patients with pulmonary manifestations being the most common presentation. We present a case of a young HIV-negative male who required 4 exploratory laparotomies over the course of 4 months during 2 hospitalizations due to discrete perforations of the ileum and jejunum caused by biopsy-proven gastrointestinal histoplasmosis despite maximal medical therapy as well as a gastric perforation.


Asunto(s)
Histoplasmosis , Perforación Intestinal , Humanos , Masculino , Histoplasmosis/diagnóstico , Histoplasmosis/complicaciones , Perforación Intestinal/etiología , Perforación Intestinal/microbiología , Perforación Intestinal/cirugía , Adulto , Seronegatividad para VIH , Enfermedades del Íleon/microbiología , Enfermedades del Íleon/etiología , Enfermedades del Íleon/diagnóstico , Enfermedades del Yeyuno/etiología , Enfermedades del Yeyuno/microbiología , Enfermedades del Yeyuno/diagnóstico
3.
Front Immunol ; 12: 696148, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34603279

RESUMEN

As the first line of defense against intestinal bacteria and toxins, intestinal epithelial cells are always exposed to bacteria or lipopolysaccharide (LPS), whereas pathogenic bacteria or LPS can cause intestinal epithelial cell damage. Previous studies have shown that konjac mannan oligosaccharides (KMOS) have a positive effect on maintaining intestinal integrity, and Bacillus subtilis (BS) can promote the barrier effect of the intestine. However, it is still unknown whether KMOS and BS have a synergistic protective effect on the intestines. In this study, we used the LPS-induced Caco-2 cell injury model and mouse intestinal injury model to study the synergistic effects of KMOS and BS. Compared with KMOS or BS alone, co-treatment with KMOS and BS significantly enhanced the activity and antioxidant capacity of Caco-2 cell, protected mouse liver and ileum from LPS-induced oxidative damage, and repaired tight junction and mucus barrier damage by up-regulating the expression of Claudin-1, ZO-1 and MUC-2. Our results demonstrate that the combination of KMOS and BS has a synergistic repair effect on inflammatory and oxidative damage of Caco-2 cells and aIIeviates LPS-induced acute intestinal injury in mice.


Asunto(s)
Bacillus subtilis/fisiología , Células Epiteliales/efectos de los fármacos , Células Epiteliales/microbiología , Enfermedades del Íleon/prevención & control , Mucosa Intestinal/efectos de los fármacos , Mucosa Intestinal/microbiología , Mananos/farmacología , Probióticos , Animales , Células CACO-2 , Supervivencia Celular/efectos de los fármacos , Enfermedad Hepática Inducida por Sustancias y Drogas/metabolismo , Enfermedad Hepática Inducida por Sustancias y Drogas/microbiología , Enfermedad Hepática Inducida por Sustancias y Drogas/patología , Enfermedad Hepática Inducida por Sustancias y Drogas/prevención & control , Modelos Animales de Enfermedad , Células Epiteliales/metabolismo , Células Epiteliales/patología , Femenino , Humanos , Enfermedades del Íleon/metabolismo , Enfermedades del Íleon/microbiología , Enfermedades del Íleon/patología , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patología , Lipopolisacáridos , Ratones , Estrés Oxidativo/efectos de los fármacos , Permeabilidad , Uniones Estrechas/efectos de los fármacos , Uniones Estrechas/metabolismo , Uniones Estrechas/microbiología , Uniones Estrechas/patología
4.
BMJ Case Rep ; 14(2)2021 Feb 09.
Artículo en Inglés | MEDLINE | ID: mdl-33563666

RESUMEN

Tuberculosis (TB), a significant cause of morbidity and mortality worldwide, is particularly relevant in low/middle-income countries like India, where the disease is endemic. The female reproductive system is very vulnerable to this infection with, the clinical presentation being utterly silent in most patients. Symptoms of TB in pregnancy may initially be attributed to the gravidity itself besides temporary concealment of associated weight loss by the normally occurring weight gain during the pregnancy. Untreated TB may cause pregnancy loss by either placental damage or direct harm to both the mother and child. We report a case of latent disseminated TB in a young immunocompetent female that was revealed in the postpartum state (after full-term stillbirth delivery at home) as 20 ileal perforations secondary to intestinal TB. Due to ongoing sepsis and delayed presentation to the hospital, the patient could not be salvaged despite the best possible efforts.


Asunto(s)
Enfermedades del Íleon/microbiología , Perforación Intestinal/microbiología , Complicaciones Infecciosas del Embarazo/microbiología , Mortinato , Tuberculosis Gastrointestinal/complicaciones , Adulto , Resultado Fatal , Femenino , Humanos , Embarazo
5.
BMJ Case Rep ; 13(9)2020 Sep 02.
Artículo en Inglés | MEDLINE | ID: mdl-32878854

RESUMEN

A 22-year-old young woman presented with fever, lower abdominal pain and vomiting for 20 days. She had persistent fever and abdominal pain. Fever panel was negative. Clinical features were suggestive of subacute small bowel obstruction. Contrast-enhanced CT abdomen showed thickening of distal ileum, ileocaecal junction and caecum with conglomerate necrotic nodal mass in the ileocolic mesentry along with a lesion in the tail of pancreas. Patient was discussed with multidisciplinary team and decided to undergo a single-stage procedure after adequate nutritional optimisation. During optimisation, she underwent acute obstruction and hence taken up for emergency laparotomy proceeded to right haemicolectomy with distal pancreatectomy and splenectomy 4 weeks after the time of admission. Histopathology showed ileocaecal tuberculosis and solid pseudopapillary tumour with margins free of tumour. Approach of obstructed ileocaecal tuberculosis in the setting of incidental diagnosis of solid pseudopapillary tumour of pancreas in a moribund patient was challenging.


Asunto(s)
Enfermedades del Íleon/terapia , Obstrucción Intestinal/cirugía , Neoplasias Pancreáticas/cirugía , Tuberculosis Gastrointestinal/terapia , Tuberculosis Esplénica/terapia , Tiflitis/terapia , Dolor Abdominal/etiología , Antituberculosos/uso terapéutico , Colectomía , Terapia Combinada/métodos , Femenino , Humanos , Enfermedades del Íleon/complicaciones , Enfermedades del Íleon/diagnóstico , Enfermedades del Íleon/microbiología , Hallazgos Incidentales , Obstrucción Intestinal/diagnóstico , Obstrucción Intestinal/etiología , Mycobacterium tuberculosis/aislamiento & purificación , Pancreatectomía , Neoplasias Pancreáticas/diagnóstico , Esplenectomía , Tomografía Computarizada por Rayos X , Tuberculosis Gastrointestinal/complicaciones , Tuberculosis Gastrointestinal/diagnóstico , Tuberculosis Gastrointestinal/microbiología , Tuberculosis Esplénica/complicaciones , Tuberculosis Esplénica/diagnóstico , Tuberculosis Esplénica/microbiología , Tiflitis/complicaciones , Tiflitis/diagnóstico , Tiflitis/microbiología , Vómitos/etiología , Adulto Joven
9.
Rev. esp. patol ; 51(4): 253-256, oct.-dic. 2018. ilus
Artículo en Español | IBECS | ID: ibc-179172

RESUMEN

La actinomicosis es una enfermedad crónica, supurativa e inflamatoria granulomatosa causada por bacterias filamentosas grampositivas anaerobias del género Actinomyces, siendo más frecuente Actinomyces israelii. Caso clínico: hombre de 42 años de edad con dolor abdominal, pérdida de peso de 10kg y tumor en epigastrio adherido a planos profundos. La tomografía reveló dilatación de las asas intestinales; así como engrosamiento de las paredes de colon, por lo que se pensó en proceso linfoproliferativo. Se extirparon quirúrgicamente el tumor, fragmento íleon y colon; los cuales se encontraban adheridos por tejido fibroadiposo de la serosa. Microscópicamente se observó abundante infiltrado de leucocitos polimorfonucleares y gránulos de bacterias compatibles con Actinomyces spp


Actinomycosis is a chronic, suppurative, inflammatory granulomatous disease caused by gram positive anaerobic filamentous bacteria of the genus Actinomyces, most frequently Actinomyces israelii. We report a case of a 42-year-old male presenting with abdominal pain, a 10kg weight loss and a fixed mass in the epigastrium. Tomography revealed dilatation of the intestinal loops and thickening of the colon walls; the coexistence of these two findings suggested a lymphoproliferative process. The tumour, ileum fragment and colon were surgically removed; these were adherent to the serosal fibro-adipose tissue. Microscopically, abundant polymorphonuclear infiltrate and grains of bacteria compatible with Actinomyces spp.were seen


Asunto(s)
Humanos , Masculino , Adulto , Actinomicosis/diagnóstico , Enfermedades del Íleon/microbiología , Enfermedades del Colon/microbiología , Neoplasias Abdominales/patología , Actinomyces/patogenicidad , Enfermedades Intestinales/microbiología , Neoplasias Abdominales/complicaciones
12.
Pan Afr Med J ; 23: 148, 2016.
Artículo en Francés | MEDLINE | ID: mdl-27279973

RESUMEN

Some cases of suppurative mesenteric adenitis have already been described in the literature but not associated with intussusception. We describe the case of a 3-year-old boy presenting to the department of surgery at the University Hospital of Lubumbashi with bowel obstruction. He was visited elsewhere, in the previous 12 days, for diarrhea, vomiting, fever, coma and treated for cerebral malaria and blackwater fever. Surgery revealed an ileal intussusception and a suppurative mesenteric adenitis whose pyoculture revealed the presence of Enterobacter cloacae, sensitive to norfloxacin. We performed desinvagination, sucked the pus out into a syringe and excized completely the site of suppurative adenitis. The evolution of patient was good. The clinician must know that the association between suppurative mesenteric adenitis and intussusceptions exists. The diagnosis is not easy and there is the risk of developing acute peritonitis due to its fistulation in the abdominal cavity.


Asunto(s)
Enfermedades del Íleon/diagnóstico , Obstrucción Intestinal/etiología , Intususcepción/diagnóstico , Linfadenitis Mesentérica/diagnóstico , Preescolar , Enterobacter cloacae/aislamiento & purificación , Infecciones por Enterobacteriaceae/diagnóstico , Infecciones por Enterobacteriaceae/patología , Humanos , Enfermedades del Íleon/complicaciones , Enfermedades del Íleon/microbiología , Obstrucción Intestinal/cirugía , Intususcepción/complicaciones , Intususcepción/microbiología , Masculino , Linfadenitis Mesentérica/complicaciones , Linfadenitis Mesentérica/microbiología , Supuración/diagnóstico
13.
PLoS One ; 11(4): e0153932, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-27093613

RESUMEN

Johne's disease is a chronic infection of the small intestine caused by Mycobacterium avium subspecies paratuberculosis (MAP), an intracellular bacterium. The events of pathogen survival within the host cell(s), chronic inflammation and the progression from asymptomatic subclinical stage to an advanced clinical stage of infection, are poorly understood. This study examines gene expression in the ileocecal valve (ICV) of Holstein dairy cows at different stages of MAP infection. The ICV is known to be a primary site of MAP colonization and provides an ideal location to identify genes that are relevant to the progression of this disease. RNA was prepared from ICV tissues and RNA-Seq was used to compare gene transcription between clinical, subclinical, and uninfected control animals. Interpretation of the gene expression data was performed using pathway analysis and gene ontology categories containing multiple differentially expressed genes. Results demonstrated that many of the pathways that had strong differential gene expression between uninfected control and clinical cows were related to the immune system, such as the T- and B-cell receptor signaling, apoptosis, NOD-like receptor signaling, and leukocyte transendothelial migration pathways. In contrast, the comparison of gene transcription between control and subclinical cows identified pathways that were primarily involved in metabolism. The results from the comparison between clinical and subclinical animals indicate recruitment of neutrophils, up regulation of lysosomal peptidases, increase in immune cell transendothelial migration, and modifications of the extracelluar matrix. This study provides important insight into how cattle respond to a natural MAP infection at the gene transcription level within a key target tissue for infection.


Asunto(s)
Enfermedades del Íleon/microbiología , Válvula Ileocecal/microbiología , Mycobacterium avium subsp. paratuberculosis/inmunología , Transcripción Genética/genética , Animales , Apoptosis/inmunología , Linfocitos B/inmunología , Linfocitos B/microbiología , Bovinos , Enfermedades de los Bovinos/inmunología , Enfermedades de los Bovinos/microbiología , Movimiento Celular/inmunología , Células Endoteliales/inmunología , Células Endoteliales/microbiología , Matriz Extracelular/inmunología , Matriz Extracelular/microbiología , Expresión Génica/genética , Expresión Génica/inmunología , Perfilación de la Expresión Génica/métodos , Enfermedades del Íleon/inmunología , Válvula Ileocecal/inmunología , Leucocitos Mononucleares/inmunología , Leucocitos Mononucleares/microbiología , Paratuberculosis/inmunología , Paratuberculosis/microbiología , Transducción de Señal/inmunología , Linfocitos T/inmunología , Linfocitos T/microbiología , Transcripción Genética/inmunología
14.
Physiol Rep ; 4(6)2016 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-27033447

RESUMEN

NSAIDuse is limited due to the drugs' toxicity to the gastrointestinal mucosa, an action incompletely understood. Lower gut injury induced byNSAIDs is dependent on bile secretion and is reported to increase the growth of a number of bacterial species, including an enterococcal species,Enterococcus faecalis This study examined the relationships between indomethacin (INDO)-induced intestinal injury/bleeding, small bowel overgrowth (SBO) and dissemination of enterococci, and the contribution of bile secretion to these pathological responses. Rats received either a sham operation (SO) or bile duct ligation (BDL) prior to administration of two daily subcutaneous doses of saline orINDO, and 24 h later, biopsies of ileum and liver were collected for plating on selective bacterial media. Fecal hemoglobin (Hb) and blood hematocrit (Hct) were measured to assess intestinal bleeding. Of the four treatment groups, onlySO/INDOrats experienced a significant 10- to 30-fold increase in fecal Hb and reduction in Hct, indicating thatBDLattenuatedINDO-induced intestinal injury/bleeding. Ileal enterococcal colony-forming units were significantly increased (500- to 1000-fold) inSO/INDOrats. Of all groups, only theSO/INDOrats demonstrated gut injury, and this was associated with enterococcal overgrowth of the gut and dissemination to the liver. We also demonstrated thatINDO-induced intestinal injury andE. faecalisovergrowth was independent of the route of administration of the drug, as similar findings were observed in rats orally dosed with theNSAID Bile secretion plays an important role inINDO-induced gut injury and appears to support enterococcal overgrowth of the intestine.NSAID-induced enterococcalSBOmay be involved either as a compensatory response to gut injury or with the pathogenic process itself and the subsequent development of sepsis.


Asunto(s)
Antiinflamatorios no Esteroideos , Conductos Biliares/metabolismo , Bilis/metabolismo , Enterococcus faecalis/crecimiento & desarrollo , Hemorragia Gastrointestinal/microbiología , Enfermedades del Íleon/microbiología , Íleon/microbiología , Indometacina , Animales , Traslocación Bacteriana , Conductos Biliares/cirugía , Modelos Animales de Enfermedad , Enterococcus faecalis/metabolismo , Heces/química , Hemorragia Gastrointestinal/inducido químicamente , Hemorragia Gastrointestinal/metabolismo , Hemorragia Gastrointestinal/patología , Hemoglobinas/metabolismo , Enfermedades del Íleon/inducido químicamente , Enfermedades del Íleon/metabolismo , Enfermedades del Íleon/patología , Íleon/metabolismo , Íleon/patología , Ligadura , Hígado/microbiología , Masculino , Ratas Sprague-Dawley
15.
Rev Med Interne ; 37(10): 705-707, 2016 Oct.
Artículo en Francés | MEDLINE | ID: mdl-26971967

RESUMEN

INTRODUCTION: Intestinal symptoms (cramping, flatulence) and iron deficient anemia are classical presenting manifestations of duodenal hookworm infestation in patients living in endemic area. CASE REPORT: We report a 45-year-old immunocompetent metropolitan man who presented with intestinal obstruction secondary to massive hookworm infestation complicated by fatal plurimicrobial bacteriemia with refractory septic shock. CONCLUSION: We report a case of acute surgical abdominal presentation with septicemia and refractory shock syndrome due to ileal translocation secondary to massive hookworm infestation. To the best of our knowledge, such a case has not yet been reported.


Asunto(s)
Bacteriemia/microbiología , Infecciones por Uncinaria/complicaciones , Enfermedades del Íleon/microbiología , Enfermedades del Íleon/parasitología , Obstrucción Intestinal/microbiología , Obstrucción Intestinal/parasitología , Bacteriemia/complicaciones , Bacteriemia/parasitología , Resultado Fatal , Infecciones por Uncinaria/microbiología , Humanos , Enfermedades del Íleon/complicaciones , Enfermedades del Íleon/patología , Obstrucción Intestinal/complicaciones , Obstrucción Intestinal/patología , Intestino Delgado/microbiología , Intestino Delgado/parasitología , Masculino , Persona de Mediana Edad , Sepsis/complicaciones , Sepsis/microbiología , Sepsis/parasitología
16.
Inflamm Bowel Dis ; 22(2): 293-302, 2016 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-26752462

RESUMEN

BACKGROUND: The etiology of inflammatory bowel disease is believed to involve a shift in the microbiota toward more proinflammatory species. Crohn's disease (CD) usually manifests as one of three phenotypes, involving inflammation of the terminal ileum, the colon, or both. However, what determines the particular phenotype and the level of disease activity remains unknown. In this study, we aim to characterize the intestinal microbiota associated with different CD phenotypes. METHODS: DNA was extracted from biopsies of 31 patients with ileal, ileocolic, or colon-restricted CD, and also from 5 non-inflammatory bowel disease control subjects, and analyzed by 16S rRNA gene amplicon pyrosequencing. Data were processed using the Quantitative Insights Into Microbial Ecology pipeline and analyzed using linear discriminant analysis with effect size estimation and PICRUSt algorithms. Two additional recently published cohorts were also analyzed in this study. RESULTS: Highly significant separation was observed between bacterial composition of ileal CD compared with CD with colonic involvement (genus level Bray-Curtis P = 0.005, R = 20%). This separation was unaffected by the biopsy's location or its inflammatory state, or by the patients' condition (remission or relapse). Faecalibacterium was strongly reduced in ileal CD compared with CD with colonic involvement, whereas Enterobacteriaceae were more abundant in the former. Fusobacterium relative abundance was strongly correlated with disease activity in patients with ileal-involving, but not in colon-involving, CD. CONCLUSIONS: Ileal and colon-involving CD sustain distinct microbiotas, suggesting that different mechanisms underlie the two major manifestations of CD. The potential contribution of Fusobacterium to inflammation in ileal CD should be further investigated.


Asunto(s)
Colon/microbiología , Enfermedad de Crohn/microbiología , Enfermedades del Íleon/microbiología , Íleon/microbiología , Inflamación/microbiología , Microbiota/genética , Adulto , Estudios de Casos y Controles , Colon/patología , Enfermedad de Crohn/genética , Enfermedad de Crohn/patología , Femenino , Estudios de Seguimiento , Humanos , Enfermedades del Íleon/genética , Enfermedades del Íleon/patología , Íleon/patología , Inflamación/genética , Inflamación/patología , Masculino , Persona de Mediana Edad , Pronóstico , ARN Ribosómico 16S/genética
17.
Genet Mol Res ; 14(4): 14387-95, 2015 Nov 19.
Artículo en Inglés | MEDLINE | ID: mdl-26600498

RESUMEN

This study aimed to summarize our experience in surgical treatment of mesh infection after repair of ventral hernia or defect. A retrospective analysis was conducted on clinical data of 22 patients who accepted surgical treatment of mesh infection after ventral hernia or defect repair. Included were 16 cases of infection after incisional hernia repair, 5 cases of infection after abdominal wall defect repair following abdominal wall tumor resection, and 1 case of infection with fistula caused by a parastomal hernia of an ileal neobladder repair with a prosthetic patch. All patients had received local dressing treatment for 2 to 24 months but were not healed. The affected mesh was removed successfully in all patients. Six patients had abdominal wall repair using the component separation technique; 4 patients were treated by strengthened repair with polypropylene mesh; 10 patients were repaired with human acellular dermal matrix; 1 patient received local dressing changes and vacuum sealing drain treatment without repair; and 1 patient received wound closure without strengthened repair. The postoperative hospital stay was 9-29 days (mean 16 days). After treatment, 19 patients recovered with primary wound healing and 3 patients recovered with secondary healing. All patients were followed up for 6-38 months (mean 26 months), and no ventral hernia or defect recurred except 1 case of lower abdominal bulge. Mesh infections after ventral hernia or defect repair are difficult to treat using prosthetic materials. For satisfactory results, surgery should be performed according to the specific condition of the individual.


Asunto(s)
Hernia Ventral/cirugía , Enfermedades del Íleon/cirugía , Infección de la Herida Quirúrgica/cirugía , Cicatrización de Heridas , Adulto , Anciano , Femenino , Hernia Ventral/microbiología , Hernia Ventral/patología , Humanos , Enfermedades del Íleon/microbiología , Enfermedades del Íleon/patología , Masculino , Persona de Mediana Edad , Prótesis e Implantes , Recurrencia , Mallas Quirúrgicas/microbiología , Infección de la Herida Quirúrgica/microbiología , Infección de la Herida Quirúrgica/patología
18.
Tech Coloproctol ; 19(12): 717-27, 2015 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-26385573

RESUMEN

The pathological diagnosis of inflammatory bowel disease (IBD) is often difficult because biopsy material may not contain pathognomonic features, making distinction between Crohn's disease, ulcerative colitis and other forms of colitides a truly challenging exercise. The problem is further complicated as several diseases frequently mimic the histological changes seen in IBD. Successful diagnosis is reliant on careful clinicopathological correlation and recognising potential pitfalls. This is best achieved in a multidisciplinary team setting when the full clinical history, endoscopic findings, radiology and relevant serology and microbiology are available. In this review, we present an up-to-date evaluation of the histopathological mimics of IBD.


Asunto(s)
Enfermedades del Ciego/patología , Colitis/patología , Colon/efectos de la radiación , Enfermedades del Íleon/patología , Enfermedades Inflamatorias del Intestino/patología , Traumatismos por Radiación/patología , Tuberculosis Gastrointestinal/patología , Antiinflamatorios no Esteroideos/efectos adversos , Enfermedades del Ciego/microbiología , Colitis/etiología , Colon/irrigación sanguínea , Colon/patología , Diagnóstico Diferencial , Divertículo/complicaciones , Entamebiasis/complicaciones , Entamebiasis/patología , Enfermedad Injerto contra Huésped/complicaciones , Enfermedad Injerto contra Huésped/patología , Humanos , Enfermedades del Íleon/microbiología , Isquemia/complicaciones , Linfogranuloma Venéreo/complicaciones , Linfogranuloma Venéreo/patología , Linfoma/complicaciones , Linfoma/patología , Reservoritis/patología , Infecciones por Yersinia pseudotuberculosis/complicaciones , Infecciones por Yersinia pseudotuberculosis/patología
19.
World J Gastroenterol ; 21(9): 2638-44, 2015 Mar 07.
Artículo en Inglés | MEDLINE | ID: mdl-25759531

RESUMEN

AIM: To investigate the protective effect of bifidobacterium in endotoxin-induced intestinal injury in preweaning rats. METHODS: Preweaning rats were randomly divided into three groups (n = 40 for each): a control group (group C), a model group (group E) and a treatment group (group T). Both groups E and T were intraperitoneally injected with lipopolysaccharide (LPS) at a dose of 5 mg/kg (5 mg/L in normal saline), and group T was intragastrically administrated with bifidobacterium suspension (2.0 × 10(9) CFU/mL, 0.5 mL each time, twice a day, until the end of the experiment) 7 d before LPS administration. Group C was intraperitoneally injected with normal saline. After intraperitoneal injection and intragastric administration, the rats were placed back to the initial cage to receive breast feeding. The rats were killed at 2, 6, 12, 24 or 72 h, respectively, after endotoxin or physiological saline injection to collect serum and ileal tissue samples. Myeloperoxidase (MPO) contents in serum and ileum were detected at different times, and expression of ileal defensin-5 mRNA was evaluated by reverse transcription-polymerase chain reaction. RESULTS: Serum and ileal MPO contents in group E were significantly higher than those in group C (serum contents: 107.50 ± 17.70 vs 157.14 ± 24.67, P < 0.05; ileal contents: 1.03 ± 0.21 vs 1.57 ± 0.33, P < 0.05), which peaked at 12 h and 6 h, respectively. MPO contents in group T were significantly lower than those in group E (serum contents: 114.38 ± 24.56 vs 145.25 ± 23.62, P < 0.05; ileal contents: 1.25 ± 0.24 vs 1.57 ± 0.33, P < 0.05). The expression of defensin-5 mRNA in group E was significantly higher than that in group C (0.953 ± 0.238 vs 0.631 ± 0.146, P < 0.05), which peaked at 2 h, and then decreased gradually. The expression of defensin-5 mRNA in group T was significantly lower than that in group E (0.487 ± 0.149 vs 0.758 ± 0.160, P < 0.05) apparently in 24 h. The expression of defensin-5 mRNA at 2 h in group T was significantly higher than that in group C (0.824 ± 0.158 vs 0.631 ± 0.146, P < 0.05). CONCLUSION: MPO and defensin-5 mRNA increase in preweaning rats with LPS-induced intestinal injury. Bifidobacterium protects the gut by inhibiting MPO activity, not by increasing defensin-5 secretion.


Asunto(s)
Bifidobacterium/fisiología , Defensinas/metabolismo , Enfermedades del Íleon/prevención & control , Íleon/metabolismo , Íleon/microbiología , Probióticos , Animales , Animales Recién Nacidos , Defensinas/genética , Modelos Animales de Enfermedad , Femenino , Enfermedades del Íleon/inducido químicamente , Enfermedades del Íleon/genética , Enfermedades del Íleon/metabolismo , Enfermedades del Íleon/microbiología , Lactancia , Lipopolisacáridos , Peroxidasa/sangre , ARN Mensajero/metabolismo , Ratas Wistar , Factores de Tiempo
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