RESUMEN
ABSTRACT: Primary cutaneous acral CD8-positive T-cell lymphoma consists of slow-growing nodules in acral sites with a histopathology, suggesting high-grade lymphoma despite the indolent clinical course. It has been recently included in WHO-EORTC classification for primary cutaneous lymphomas as a provisional entity. A correct diagnosis of this entity is important because its differential diagnosis include more aggressive cutaneous lymphomas. We present a 53-year-old woman with an indolent solitary nodule on her right leg, which histopathologically showed features of CD8-positive T-cell lymphoma, although with some peculiarities, including epidermotropism, absence of CD68 expression, and positivity for GATA3 and Bcl6 in neoplastic cells. This case could contribute to better define the spectrum of this rare cutaneous lymphoma.
Asunto(s)
Biomarcadores de Tumor/análisis , Linfocitos T CD8-positivos/química , Factor de Transcripción GATA3/análisis , Linfocitos Infiltrantes de Tumor/química , Linfoma Cutáneo de Células T/química , Proteínas Proto-Oncogénicas c-bcl-6/análisis , Neoplasias Cutáneas/química , Biopsia , Linfocitos T CD8-positivos/inmunología , Femenino , Humanos , Inmunohistoquímica , Linfocitos Infiltrantes de Tumor/inmunología , Linfoma Cutáneo de Células T/inmunología , Linfoma Cutáneo de Células T/patología , Linfoma Cutáneo de Células T/cirugía , Persona de Mediana Edad , Neoplasias Cutáneas/inmunología , Neoplasias Cutáneas/patología , Neoplasias Cutáneas/cirugía , Resultado del TratamientoAsunto(s)
Neoplasias Encefálicas/secundario , Linfocitos T CD8-positivos/patología , Dermatomiositis/diagnóstico , Linfoma Cutáneo de Células T/patología , Neoplasias Cutáneas/patología , Adolescente , Protocolos de Quimioterapia Combinada Antineoplásica/administración & dosificación , Biomarcadores de Tumor/análisis , Biopsia , Neoplasias Encefálicas/química , Neoplasias Encefálicas/inmunología , Neoplasias Encefálicas/terapia , Linfocitos T CD8-positivos/química , Linfocitos T CD8-positivos/inmunología , Trasplante de Células Madre de Sangre del Cordón Umbilical , Ciclofosfamida/administración & dosificación , Diagnóstico Diferencial , Doxorrubicina/administración & dosificación , Etopósido/administración & dosificación , Humanos , Linfoma Cutáneo de Células T/química , Linfoma Cutáneo de Células T/inmunología , Linfoma Cutáneo de Células T/terapia , Imagen por Resonancia Magnética , Masculino , Valor Predictivo de las Pruebas , Prednisona/administración & dosificación , Neoplasias Cutáneas/química , Neoplasias Cutáneas/inmunología , Neoplasias Cutáneas/terapia , Trasplante Homólogo , Resultado del Tratamiento , Vincristina/administración & dosificaciónRESUMEN
Folliculotropic mycosis fungoides (FMF), a variant of mycosis fungoides (MF) with distinct clinical features, is characterized by infiltration of malignant T cells in hair follicles. This raises the hypothesis that antigens in the hair follicle may contribute to the pathogenesis of FMF. T-cell receptor ß gene (TRB) sequences as well as dendritic cell subsets in patients with FMF (n = 21) and control patients with MF (n = 20) were studied to explore this hypothesis. A recurrent usage of the TRB junctional genes TRBJ2-1 and TRBJ2-7 was found in patients with FMF compared with those with MF. These genes contribute to an amino acid motif in the complementarity-determining region 3 (CDR3) of the T-cell receptor. This motif was previously found in T cells stimulated by lipids bound to CD1 on antigen-presenting cells. Additional immunohistochemical analysis revealed abundant CD1c- and CD1a- expressing dendritic cells in FMF. The combined findings support a role for lipid-antigen stimulation in FMF.
Asunto(s)
Antígenos CD1/análisis , Glicoproteínas/análisis , Micosis Fungoide/química , Micosis Fungoide/patología , Estudios de Casos y Controles , Regiones Determinantes de Complementariedad/análisis , Células Dendríticas/química , Genes Codificadores de la Cadena beta de los Receptores de Linfocito T , Folículo Piloso/química , Folículo Piloso/patología , Humanos , Inmunohistoquímica , Linfoma Cutáneo de Células T/química , Linfoma Cutáneo de Células T/genética , Linfoma Cutáneo de Células T/patología , Micosis Fungoide/genética , Neoplasias Cutáneas/química , Neoplasias Cutáneas/genética , Neoplasias Cutáneas/patologíaRESUMEN
T lymphocytes belong to 2 distinct sublineages that express either αß or γδ T-cell receptor (TCR) complex. Although malignancy is a great instigator of lineage infidelity, as exemplified by aberrant expression of numerous lineage markers in lymphoma cells, malignant T cells rarely coexpress αß and γδ TCR complexes. Similarly, only rare cases of CD4/CD8 double-positive primary cutaneous T-cell lymphoma have been reported. In this report, we describe a remarkable case of primary cutaneous T-cell lymphoma coexpressing αß and γδ TCR complexes, strong diffuse CD8, and a very restricted coexpression of CD4 and CD8. A 66-year-old man was referred to our center for treatment of a persistent eczematoid eruption of 6 years of duration. An initial biopsy demonstrated not only marked spongiosis, but also an epidermotropic population of CD4 small mature T cells with partial expression of CD8. The process remained indolent for another year, followed by an abrupt progression with development of plaques and tumors. Repeat biopsies of these lesions demonstrated a superimposed population of large anaplastic T cells extensively involving the dermis and epidermis. The large cells showed a strong uniform expression of CD3, CD8, CD45RA, CD5, granzyme, TIA1, perforin, TCR-ß, and TCR-γ and a weaker but unambiguous expression of CD4, CD25, CD2, and CD56. TCR gene rearrangement studies showed clonal rearrangements for TCR-ß and TCR-γ with identical peaks to those seen in the biopsy from a year earlier. The patient developed lymphadenopathy, with a biopsy showing nodal involvement by a morphologically and phenotypically identical neoplastic T-cell population. The disease showed partial response to systemic chemotherapy with development of new plaques, but these new lesions have regressed with radiation therapy.
Asunto(s)
Antígenos CD/análisis , Linfoma Cutáneo de Células T/química , Linfoma Cutáneo de Células T/patología , Receptores de Antígenos de Linfocitos T alfa-beta/análisis , Receptores de Antígenos de Linfocitos T gamma-delta/análisis , Anciano , Granzimas/análisis , Humanos , Masculino , Perforina/análisis , Proteínas de Unión a Poli(A)/análisis , Antígeno Intracelular 1 de las Células TRESUMEN
Cutaneous peripheral T-cell lymphomas not otherwise specified (CPTL-NOS) are rare neoplasms accounting for just 2% of cutaneous peripheral T-cell lymphomas (CPTL). Only very few case series have been reported. They represent a phenotypically and prognostically heterogenous group of CPTL that do not fit into any of CPTL well-defined subtypes. The authors report a case of a 64-year-old man with simultaneous plaque-like lesions and disseminated nodules growing rapidly on the face, trunk, and extremities over a 6-month period. There was no a history of preceding patches, erythematous plaques, rash, or pruritic lesions. These lesions were extending over 80% of the skin surface. Histopathologic analysis revealed dense diffuse infiltrates composed of mostly medium-sized to large lymphoid cells throughout the entire dermis without epidermotropism. Neoplastic cells were atypical with markedly pleomorphic nuclei. Immunohistochemistry showed that the tumor cells were positive for CD3, CD4, and CD5 with a loss of CD7. They were negative for CD20, CD8, CD56, CXCL13, PD1, TIA-1, granzyme-B, perforin, CD25, and CD30. The proliferative fraction was low, with MIB-1 labeling less than 10% of cells. The authors diagnosed the patient with primary CPTL-NOS. Despite the rarity of these tumors, clinicians as well as dermatopathologists and pathologists should be familiar with these rare CPTL especially because most of these lymphomas have an aggressive behavior and exhibit an unfavorable prognosis.
Asunto(s)
Linfoma Cutáneo de Células T/química , Linfoma Cutáneo de Células T/patología , Antígenos CD/análisis , Quimiocina CXCL13/análisis , Granzimas/análisis , Humanos , Masculino , Persona de Mediana Edad , Perforina/análisis , Proteínas de Unión a Poli(A)/análisis , Receptor de Muerte Celular Programada 1/análisis , Antígeno Intracelular 1 de las Células TRESUMEN
Hydroa vacciniforme-like lymphoma is a recently recognized cutaneous T-cell lymphoma associated with Epstein-Barr virus. The disease is observed in children of Latin American or Asian ethnicity. The authors report the clinical, histopathological, and immunophenotypical features of 9 new Mexican patients (M:F = 2:1; mean age, 14.5 years; median age, 13.3 years; age range, 4-27 years), expanding on previous observations of this elusive disease. The most common clinical aspects were persistent facial edema with necroses and pitted scars. Histopathological analyses revealed variably dense lymphoid infiltrates with common angiodestructive features. Neoplastic cells expressed CD3 and cytotoxic markers in all cases and were constantly positive for Epstein-Barr virus (EBER-1). Expression of other markers was variable. Follow-up data revealed that all patients died within 6 months or less, thus showing a very aggressive course with poor prognosis.
Asunto(s)
Edema/patología , Infecciones por Virus de Epstein-Barr/complicaciones , Cara/patología , Neoplasias Faciales/patología , Hidroa Vacciniforme/patología , Linfoma Cutáneo de Células T/patología , Adolescente , Adulto , Complejo CD3/análisis , Niño , Preescolar , Cicatriz/patología , Cicatriz/virología , Edema/virología , Extremidades/patología , Neoplasias Faciales/química , Neoplasias Faciales/virología , Femenino , Humanos , Hidroa Vacciniforme/virología , Inmunohistoquímica , Linfoma Cutáneo de Células T/química , Linfoma Cutáneo de Células T/virología , Masculino , México , Necrosis/patología , Necrosis/virología , Pronóstico , Torso/patología , Adulto JovenRESUMEN
Published reports of spontaneous neoplasia in marsh rice rats (Oryzomys palustris) are sparse. We report here a case of cutaneous epitheliotropic T-cell lymphoma in a 14-mo-old marsh rice rat that involved the ear pinnae, with dissemination to the liver and spleen. Histologically, the thickened ear pinnae showed diffuse infiltration of neoplastic lymphocytes into the epidermis, dermis, and adnexal skin structures, with Pautrier microaggregations present in the epidermis. In addition, neoplastic lymphocytes were observed infiltrating and disrupting the architecture of the liver and spleen. Neoplastic lymphocytes were strongly positive for the T-cell marker CD3 but were negative for the B-cell markers CD19 and CD20. These histologic and immunohistochemical features are consistent with an epitheliotropic T-cell lymphoma, as previously reported in other species, including humans. To our knowledge, this report represents the first published case of spontaneous cutaneous epitheliotropic T-cell lymphoma in a marsh rice rat.
Asunto(s)
Pabellón Auricular/patología , Neoplasias del Oído/veterinaria , Linfoma Cutáneo de Células T/veterinaria , Sigmodontinae , Neoplasias Cutáneas/veterinaria , Animales , Biomarcadores de Tumor/análisis , Biopsia/veterinaria , Pabellón Auricular/química , Neoplasias del Oído/química , Neoplasias del Oído/patología , Inmunohistoquímica/veterinaria , Linfocitos Infiltrantes de Tumor/química , Linfocitos Infiltrantes de Tumor/patología , Linfoma Cutáneo de Células T/química , Linfoma Cutáneo de Células T/patología , Masculino , Neoplasias Cutáneas/química , Neoplasias Cutáneas/patologíaRESUMEN
Intravascular large cell lymphoma is a rare subtype of extranodal large cell lymphoma characterized by the presence of neoplastic cells within the lumina of small vessels. Most cases of intravascular large cell lymphoma have a B-cell phenotype. To date, 12 cases of intravascular natural killer (NK/)/T-cell lymphoma (IVNKL) have been reported. Our case is A 47-year-old female presented with erythematous patches and plaques on the lower extremities mimicking panniculitis clinically. A skin biopsy revealed intravascular lymphoma (IVL) with a NK/T cell phenotype (positive for CD3, and granzyme B and negative for CD20, CD4, CD8, CD5). The lymphoma cells were also positive for Epstein-Barr virus by Epstein-Barr virus-encoded RNA in situ hybridization test. Because this type of lymphoma is extremely rare, our case is documented and compared with the previously reported cases.
Asunto(s)
Linfoma Extranodal de Células NK-T/patología , Linfoma Cutáneo de Células T/patología , Paniculitis/patología , Neoplasias Cutáneas/patología , Neoplasias Vasculares/patología , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Biomarcadores de Tumor/análisis , Biopsia , Diagnóstico Diferencial , Femenino , Herpesvirus Humano 4/genética , Humanos , Inmunohistoquímica , Linfoma Extranodal de Células NK-T/química , Linfoma Extranodal de Células NK-T/tratamiento farmacológico , Linfoma Extranodal de Células NK-T/virología , Linfoma Cutáneo de Células T/química , Linfoma Cutáneo de Células T/tratamiento farmacológico , Linfoma Cutáneo de Células T/virología , Persona de Mediana Edad , Valor Predictivo de las Pruebas , ARN Viral/genética , Neoplasias Cutáneas/química , Neoplasias Cutáneas/tratamiento farmacológico , Neoplasias Cutáneas/virología , Resultado del Tratamiento , Neoplasias Vasculares/química , Neoplasias Vasculares/tratamiento farmacológico , Neoplasias Vasculares/virologíaRESUMEN
BACKGROUND: In nodal anaplastic large cell lymphoma, strong expression of galectin-3 (Gal-3) has been found, but only very few cases of primary cutaneous lymphoma have so far been examined. OBJECTIVES: To investigate 11 primary cutaneous anaplastic large cell lymphomas (PCALCL), 47 lymphomatoid papuloses (LYP) and 14 cases of transformed mycosis fungoides with CD30 expression (MF-T) for Gal-3 expression. METHODS: A Gal-3 score was applied using a photo-based morphometric evaluation program. Double staining for CD30 and Gal-3 was performed. Furthermore, we recorded the cellular and extracellular sublocalization of the signal. RESULTS: The Gal-3 expression in CD30+ tumor cells was significantly lower in MF-T in contrast to CD30+ lymphoproliferative disorders (CD30 LPD; p < 0.001), but we found no differences between PCALCL and LYP (p = 0.42). In PCALCL Gal-3 was more often localized in the cytoplasm in contrast to LYP, in which an equal distribution in the cytoplasm and the nucleus was more common (p = 0.9). CONCLUSIONS: The lower Gal-3 expression in MF-T in comparison to CD30 LPD might be an additional criterion to differentiate both entities. The different sublocalization of the Gal-3 signal might reflect a different biological function and behavior.
Asunto(s)
Galectina 3/análisis , Antígeno Ki-1/análisis , Linfoma Cutáneo de Células T/química , Linfoma Cutáneo de Células T/patología , Neoplasias Cutáneas/química , Neoplasias Cutáneas/patología , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Proteínas Sanguíneas , Núcleo Celular/química , Niño , Citoplasma/química , Femenino , Galectinas , Humanos , Linfoma Anaplásico Cutáneo Primario de Células Grandes/química , Linfoma Anaplásico Cutáneo Primario de Células Grandes/patología , Papulosis Linfomatoide/metabolismo , Papulosis Linfomatoide/patología , Masculino , Persona de Mediana Edad , Micosis Fungoide/química , Micosis Fungoide/patología , Adulto JovenRESUMEN
Angioimmunoblastic T-cell lymphoma (AITL) is the second most common type of peripheral T-cell lymphoma worldwide, and in some countries, it is the most common form. Clinically, AITL usually presents with systemic symptoms, diffuse lymphadenopathy, hepatosplenomegaly, and common laboratory abnormalities such as hypergammaglobulinemia. Rashes are seen in 50%-80% of patients. AITL derives from follicular T-helper cells (TFH), that express germinal center markers and produce hyperactivation of B-cells seen in AITL. Although the histological features of AITL in the skin could be similar to pathological findings present in lymph node biopsies, herein, we present 2 cases of AITL with histological and immunophenotypic features that were somewhat suggestive of extranodal marginal zone lymphoma. Caution is urged to exclude the possibility of a systemic T-cell lymphoma such as AITL in cutaneous and lymph node B-cell proliferations.
Asunto(s)
Linfadenopatía Inmunoblástica/patología , Linfoma de Células B de la Zona Marginal/patología , Linfoma Cutáneo de Células T/patología , Neoplasias Cutáneas/patología , Anciano , Diagnóstico Diferencial , Humanos , Linfadenopatía Inmunoblástica/complicaciones , Linfoma Cutáneo de Células T/química , Linfoma Cutáneo de Células T/complicaciones , Masculino , Persona de Mediana Edad , Neoplasias Cutáneas/química , Neoplasias Cutáneas/complicacionesRESUMEN
Angioimmunoblastic T-cell lymphoma (AITL), an uncommon variant of peripheral T-cell lymphoma, affects the skin in approximately 50% of cases. Its protean clinical and histopathological cutaneous manifestations pose a challenge in diagnosis, particularly when these precede the diagnosis of AITL on a lymph node biopsy. In this retrospective study, we compared 11 cases of AITL with cutaneous manifestations (mean age 67 years; male:female ratio 1:0.8; 24 skin biopsies) with 20 control cases of inflammatory and non-AITL lymphomatous diseases (mean age 52 years; male:female ratio 1:1.5; 26 skin biopsies). Clinical, histopathological, immunohistochemical, and molecular data were documented. New insights into the clinical evolution of cutaneous involvement by AITL (C-AITL), from early macular, through papular to nodular stages, were observed. Microscopically, a parallel increment in the density of the dermal infiltrate and in the detection of lymphocyte cytological atypia was noted over time. Identification and quantification of follicular T-helper cells (Tfh), the neoplastic lineage, by immunohistochemistry helped to separate cases of C-AITL from inflammatory controls, offering promise as a useful diagnostic adjunct. The presence of T-cell clonality did not have discriminatory value between the 2 groups. Our work suggests that the early maculopapular phase of C-AITL eludes identification on pathological grounds alone and that features such as cytological atypia and high endothelial venules lack diagnostic specificity. In the context of (1) a rash that simulates a drug/viral exanthem or an acute manifestation of a connective tissue disorder, but proves recalcitrant, (2) constitutional abnormalities and/or lymphadenopathy that persist, and (3) a Tfh cell-rich perivascular dermatitis, the diagnosis of early C-AITL can be suspected, but not confirmed, without the benefit of a lymph node biopsy. The later nodular phase of C-AITL occurring in a similar constitutional background, can usually be discerned as lymphomatous, clinically and pathologically. Here a Tfh cell-rich infiltrate is a clue to the specific diagnosis, but confirmation by a nodal evaluation remains mandatory. Despite the difficulty in establishing a diagnosis of C-AITL in its early stages, and speculation that the initial eruptions might be reactive in nature, our sequential data support the concept that these are lymphomatous ab initio. To address the diagnostic challenge presented by this disease, meaningful integration of clinical and pathological data is imperative.
Asunto(s)
Ganglios Linfáticos/patología , Linfocitos Infiltrantes de Tumor/patología , Linfoma Cutáneo de Células T/patología , Linfoma de Células T Periférico/patología , Neoplasias Cutáneas/patología , Linfocitos T Colaboradores-Inductores/patología , Adulto , Anciano , Anciano de 80 o más Años , Biomarcadores de Tumor/análisis , Biomarcadores de Tumor/genética , Biopsia , Diagnóstico Diferencial , Femenino , Humanos , Inmunohistoquímica , Ganglios Linfáticos/química , Linfocitos Infiltrantes de Tumor/química , Linfoma Cutáneo de Células T/química , Linfoma Cutáneo de Células T/genética , Linfoma de Células T Periférico/química , Linfoma de Células T Periférico/genética , Masculino , Persona de Mediana Edad , Reacción en Cadena de la Polimerasa , Valor Predictivo de las Pruebas , Pronóstico , Estudios Retrospectivos , Neoplasias Cutáneas/química , Neoplasias Cutáneas/genética , Linfocitos T Colaboradores-Inductores/químicaRESUMEN
Anaplastic large cell lymphoma (ALCL) is classified into systemic and primary cutaneous subtypes. A primary systemic ALCL present initially as cutaneous localized skin lesions is a rare presentation. We present an unusual case of a 13-year-old boy with systemic ALCL who presented with cutaneous nodules. The systemic nature of this lymphoma was confirmed by immunohistochemical positivity of anaplastic lymphoma kinase and epithelial membrane antigen. Confirmation of this diagnosis will lead to a poorer prognosis and more aggressive systemic therapy.
Asunto(s)
Linfoma Anaplásico de Células Grandes/patología , Linfoma Cutáneo de Células T/patología , Linfoma de Células T Periférico/patología , Neoplasias Cutáneas/patología , Adolescente , Quinasa de Linfoma Anaplásico , Biomarcadores de Tumor/análisis , Biopsia , Humanos , Inmunohistoquímica , Linfoma Anaplásico de Células Grandes/química , Linfoma Cutáneo de Células T/química , Linfoma de Células T Periférico/química , Masculino , Mucina-1/análisis , Valor Predictivo de las Pruebas , Pronóstico , Proteínas Tirosina Quinasas Receptoras/análisis , Neoplasias Cutáneas/químicaRESUMEN
Primary cutaneous gamma-delta T-cell lymphoma (PCGD-TCL) is a rare disorder, constituting less than 1% of primary cutaneous lymphomas. Most cases occur in adults and may present as plaques or nodules with ulceration. Here we describe an unusual case of PCGD-TCL in a 3-year-old boy who presented with asymptomatic subcutaneous nodules. To our knowledge, this report represents one of the youngest reported patients with gamma-delta lymphoma/lymphoproliferative disorder. In addition, our patient has an indolent clinical presentation with greater than 1 year clinical follow-up. Because gamma-delta T-cell lymphomas are exceedingly rare in children, we acknowledge that the clinical course/outcome in young patients is still unclear. We hope to add to the recognition that PCGD-TCLs demonstrate a wide clinical spectrum of disease with relatively indolent presentations in some cases.
Asunto(s)
Complejo CD3/análisis , Linfoma Cutáneo de Células T/química , Linfoma Cutáneo de Células T/patología , Receptores de Antígenos de Linfocitos T gamma-delta/análisis , Neoplasias Cutáneas/química , Neoplasias Cutáneas/patología , Preescolar , Humanos , Masculino , Proteínas de Unión a Poli(A)/análisis , Enfermedades Raras/metabolismo , Enfermedades Raras/patología , Antígeno Intracelular 1 de las Células TRESUMEN
Hydroa vacciniforme-like lymphoma (HVL) is a rare cutaneous T-cell lymphoma that is usually seen in children of Hispanic or Asian origin. Association between chronic latent Epstein-Barr virus infection in both hydroa vacciniforme (HV) and HVL has been demonstrated and has recently been categorized by the World Health Organization as one of the Epstein Barr virus-positive lymphoproliferative disorders of childhood. Patients with HVL present with a cutaneous rash characterized by edema, blisters, ulcers, and scars mainly seen on the face and extremities that mimic HV; however, unlike in HV, the lesions tend to be extensive and deeper and are associated with severe scarring, necrosis, and systemic manifestations. We are reporting 7 cases of an unusual clinical variant of HVL with primarily periorbital edema. All of our patients in this series presented with progressive periorbital edema that was accompanied with systemic symptoms including fever, malaise, and lymphadenopathy. Most cases were initially misinterpreted as inflammatory processes including cellulitis, arthropod bite reactions, and periorbital lupus erythematosus. The biopsy of these lesions revealed an atypical lymphocytic infiltrate predominantly distributed in the deep dermis and in subcutaneous fat. Immunohistochemistry studies revealed a cytotoxic T-cell (CD8) profile. All cases were associated with Epstein-Barr virus infection. Our study presents a rare clinical variant of HVL with predominant periorbital edema. This variant could potentially be overlooked and misdiagnosed as an inflammatory condition; thus, it needs to be included in the differential diagnosis of periorbital edema in young patients.
Asunto(s)
Edema/patología , Neoplasias del Ojo/patología , Hidroa Vacciniforme/patología , Linfoma Cutáneo de Células T/patología , Neoplasias Cutáneas/patología , Adolescente , Biomarcadores de Tumor/análisis , Biopsia , Niño , Diagnóstico Diferencial , Edema/etiología , Edema/virología , Infecciones por Virus de Epstein-Barr/complicaciones , Infecciones por Virus de Epstein-Barr/patología , Infecciones por Virus de Epstein-Barr/terapia , Infecciones por Virus de Epstein-Barr/virología , Neoplasias del Ojo/química , Neoplasias del Ojo/clasificación , Neoplasias del Ojo/terapia , Neoplasias del Ojo/virología , Femenino , Herpesvirus Humano 4/genética , Humanos , Hidroa Vacciniforme/clasificación , Hidroa Vacciniforme/terapia , Hidroa Vacciniforme/virología , Inmunohistoquímica , Hibridación in Situ , Linfoma Cutáneo de Células T/química , Linfoma Cutáneo de Células T/clasificación , Linfoma Cutáneo de Células T/terapia , Linfoma Cutáneo de Células T/virología , Masculino , Valor Predictivo de las Pruebas , ARN Viral/genética , Neoplasias Cutáneas/química , Neoplasias Cutáneas/clasificación , Neoplasias Cutáneas/terapia , Neoplasias Cutáneas/virologíaRESUMEN
Chronic actinic dermatitis/actinic reticuloid (CAD/AR) is an eczematous hypersensitivity reaction to ultraviolet rays that can vary from mild eczematous cases to AR, the most severe cases which may resemble cutaneous T-cell lymphoma. Diagnosis is based on clinical, histopathologic, and photobiologic features. In this study, we characterize the histopathologic and immunohistochemical features of 40 biopsies from 37 patients with established CAD. The cohort included 30 men and 7 women, ranging in age from 38 to 84 years (median, 62 years) and with a median duration of symptoms at presentation of 3 years (range, 1 to 40 years). All patients presented with erythematous lichenified plaques on sun-exposed areas. Severe cases (12/37) had extension to non-exposed areas. Positive photo-testing (20/20) and patch-testing (10/10) results, and cases with a high peripheral blood eosinophila (7/24) and HIV positivity (4/37) were noted. Skin biopsies demonstrated eczematous features including parakeratosis, acanthosis, spongiosis, and prominent dermal fibroplasia. Dermal dendrocytes were prominent in all cases with frequent multinucleated giant cells positive for factor XIIIa and S100 protein. Most cases displayed a brisk lymphocytic infiltrate with subtle exocytosis, atypical lymphocytes, and increased numbers of Langerhans cells, eosinophils, and plasma cells. There was a predominance of CD8 T cells within the epidermis (20/25) and a low CD4:CD8 ratio was noted in 20 of 25 cases. T-cell clonality studies were negative in 10 of 10 cases. CAD/AR may be difficult to distinguish from eczematous variants of cutaneous T-cell lymphoma. Important clues to differentiate both conditions include the identification of prominent dermal dendrocytes with multinucleated giant cells, eosinophils, plasma cells, and a low CD4:CD8 ratio.
Asunto(s)
Inmunohistoquímica , Trastornos por Fotosensibilidad/metabolismo , Trastornos por Fotosensibilidad/patología , Piel/química , Piel/patología , Adulto , Anciano , Anciano de 80 o más Años , Biomarcadores/análisis , Biopsia , Diagnóstico Diferencial , Femenino , Humanos , Linfoma Cutáneo de Células T/química , Linfoma Cutáneo de Células T/inmunología , Linfoma Cutáneo de Células T/patología , Masculino , Persona de Mediana Edad , Trastornos por Fotosensibilidad/inmunología , Valor Predictivo de las Pruebas , Piel/inmunologíaRESUMEN
Early cutaneous T cell lymphoma clinically and histologically resembles benign inflammatory skin diseases, which sometimes makes it difficult to reach a correct diagnosis. It is recently reported that thymocyte selection-associated high mobility group box factor (TOX) serves as a molecular marker for histological diagnosis of early-stage mycosis fungoides (MF). To examine whether TOX could be a marker of tumour cells in different types of cutaneous lymphoma, we investigated immunohistochemical staining for TOX with the lesional skin of patch, plaque, and tumour MF, Sézary syndrome (SS), lymphomatoid papulosis (LyP), primary cutaneous anaplastic large cell lymphoma (PCALCL), adult T cell leukemia/lymphoma (ATLL), peripheral T-cell lymphoma, not otherwise specified (PTCL, NOS), atopic dermatitis (AD), and normal skin. TOX and CCR4 messenger RNA (mRNA) levels in lesional skin of MF/SS were also examined. Immunohistological staining showed that a high specific nuclear staining of TOX was observed at a high frequency in MF, SS, and PTCL, NOS. Tumour cells in LyP, PCALCL, and ATLL showed a slightly dim nuclear staining of TOX. TOX(+) cells in MF and LyP expressed surface molecules characteristics of tumour cells in these diseases. Lesional skin of SS expressed higher levels of TOX mRNA, compared to normal skin or MF lesional skin. Moreover, TOX expression significantly correlated with CCR4 expression. TOX may be a specific marker for tumour cells in some types of cutaneous lymphoma.
Asunto(s)
Biomarcadores de Tumor/análisis , Proteínas del Grupo de Alta Movilidad/análisis , Linfoma Cutáneo de Células T/química , Neoplasias Cutáneas/química , Adulto , Anciano , Biomarcadores de Tumor/genética , Biopsia , Linfocitos T CD4-Positivos/química , Quimiocina CCL17/análisis , Quimiocina CCL17/genética , Femenino , Proteínas del Grupo de Alta Movilidad/genética , Humanos , Inmunohistoquímica , Linfocitos Infiltrantes de Tumor/química , Linfoma Cutáneo de Células T/clasificación , Linfoma Cutáneo de Células T/genética , Linfoma Cutáneo de Células T/patología , Masculino , Persona de Mediana Edad , Micosis Fungoide/química , Micosis Fungoide/genética , Micosis Fungoide/patología , ARN Mensajero/análisis , Reacción en Cadena en Tiempo Real de la Polimerasa , Receptores CCR4/análisis , Receptores CCR4/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Síndrome de Sézary/química , Síndrome de Sézary/genética , Síndrome de Sézary/patología , Neoplasias Cutáneas/clasificación , Neoplasias Cutáneas/genética , Neoplasias Cutáneas/patologíaRESUMEN
Primary cutaneous gamma-delta T-cell lymphoma (PCGD-TCL) is rare and only represents 1% of all cutaneous T-cell lymphomas. To our knowledge, only 40 cases have been described. It often presents with generalised skin lesions, preferentially affecting the extremities. There is a well-documented association with haemophagocytic syndrome. Treatment is difficult since PCGD-TCL is often resistant to chemotherapy and radiotherapy. Most case reports describe an aggressive clinical course with an estimated mean survival of 15 months. We present a 72-year-old female patient with stage IV primary cutaneous gamma-delta T-cell lymphoma. Our patient presented with fever, night sweats and multiple skin lesions (figure 1). Computed axial tomography of chest and abdomen revealed multiple solid nodular lesions in both kidneys. During admission a subconjunctival lesion appeared and progressed rapidly (figure 2). Histopathological examination of skin biopsy revealed infiltration of atypical lymphocytes with hyperchromatic irregular nuclei. Immunophenotyping pattern of skin biopsy was compatible with PCGD-TLC. Clonal gamma-delta T-cells were also detected by immunohistochemical analysis of peripheral blood and bone marrow. Polymerase chain reaction amplification revealed clonal rearrangement of the T-cell receptor gamma chain gene. These findings together were consistent with stage IV primary cutaneous gamma-delta T-cell lymphoma. The rapid progression of the subconjunctival extra-nodal manifestation is characteristic for the aggressive course of this lymphoma. Our patient was treated with two cycles of CHOP (cyclophosphamide, doxorubicin, vincristine and prednisone). However, her clinical condition deteriorated rapidly. She declined further therapy and died within three months of initial presentation.
Asunto(s)
Neoplasias del Ojo/patología , Linfoma Cutáneo de Células T/patología , Receptores de Antígenos de Linfocitos T gamma-delta/análisis , Neoplasias Cutáneas/patología , Anciano , Neoplasias del Ojo/química , Neoplasias del Ojo/genética , Resultado Fatal , Femenino , Humanos , Linfoma Cutáneo de Células T/química , Linfoma Cutáneo de Células T/genética , Receptores de Antígenos de Linfocitos T gamma-delta/genética , Neoplasias Cutáneas/química , Neoplasias Cutáneas/genéticaRESUMEN
Cutaneous γ/δ+ T-cell lymphoma (CGD-TCL) is a rare but aggressive lymphoma associated with a poor prognosis in most patients. The clinicopathological spectrum is variable including predominantly epidermotropic infiltrates manifesting with patches and plaques or tumors with dermal and/or subcutaneous infiltrates. The diagnosis of CGD-TCL requires the demonstration of a γ/δ+ phenotype by immunohistochemistry. We report 2 patients with epidermotropic cutaneous T-cell lymphomas displaying a γ/δ+ phenotype, but exhibiting an indolent course. In one patient, the clinical presentation was similar to mycosis fungoides in patch and plaque stage, but recurrent blister formation within the lesions was observed accompanied by fever and arthralgias, whereas the second patient presented with 2 localized erosive plaques on the left temple and dense epidermotropic and dermal diffuse and folliculotropic infiltrates of atypical small-to-medium-sized lymphocytes. These cases corroborate the view that expression of a γ/δ+ phenotype in cutaneous T-cell lymphomas per se does not portend a worse prognosis and that CGD-TCL may represent a clinically and prognostically heterogeneous group.