RESUMEN
Background: Platanus acerifolia (London plane tree) is a deciduous tree of the Platanaceae family. Sensitization to this plant varies withgeography. Madrid, located in central Spain, has one of the highest London plane tree pollen concentration levels on the Iberian Peninsula.Objectives: We evaluated both the clinical characteristics and the molecular sensitization pattern of patients with allergy to London planetree pollen in the region of Madrid.Patients and Methods: Thirty-eight patients allergic to London plane tree pollen were selected according to their clinical symptoms andpositive results in skin prick testing and/or specific IgE determination. Serum was collected, and allergen components were evaluatedusing immunodetection techniques as well as ImmunoCAP. The IgE-binding proteins detected were identified and characterized usingmass spectrometry.Results: Analysis of serum samples from allergic patients revealed 9 IgE-binding bands in London plane tree pollen extract. Among these,the 45-kDa protein, which corresponded to Pla a 2, was detected in 76.3% of patients. However, the 18-kDa (Pla a 1) and 9-kDa (Pla a 3)bands were detected in 44.7% and 23.7% of sera, respectively. These results were confirmed using purified proteins. Characterization ofthe allergen revealed the 27-kDa protein to be glutathione-S-transferase.Conclusions: The molecular profile of patients sensitized to London plane tree pollen differs from that reported in studies from otherlocations. In the population we studied, the prevalence of Pla a 2 was higher than that of Pla a 1 and Pla a 3. In addition, the minorallergen previously referred to as Pla a 4 was characterized as glutathione-S-transferase. (AU)
Antecedentes: Platanus acerifolia es un árbol de hoja caduca de la familia Platanaceae. La sensibilización frente a esta planta varía enfunción de la zona geográfica. Madrid, ubicada en el centro de España, tiene uno de los mayores niveles de concentración de polen deeste árbol en la Península Ibérica.Objetivo: Evaluar las características clínicas y los patrones moleculares de sensibilización en pacientes con alergia al plátano de sombraen la región de Madrid.Pacientes y Métodos: Treinta y ocho pacientes alérgicos al polen del plátano de sombra fueron seleccionados de acuerdo con los síntomasclínicos, pruebas cutáneas positivas y/o IgE específica. El suero se recogió y se evaluaron los componentes alérgicos mediante técnicas deinmunodetección, así como ImmunoCAP. Las proteínas que unían IgE fueron identificadas y caracterizadas por espectrometría de masas.Resultados: El análisis de los sueros de los pacientes alérgicos reveló 9 bandas que captaban IgE en los extractos de polen de plátano desombra. Entre estas, la proteína de 45 kDa, correspondiente a Pla a 2, se detectó en el 76,3% de los pacientes. Sin embargo, las bandasde 18 kDa (Pla a 1) y 9 kDa (Pla a 3) fueron reconocidas en el 44,7% y 27,3%, respectivamente. Estos resultados se confirmaron usandoproteínas purificadas. La caracterización de los alérgenos identificó la proteína de 27 kDa como una glutatión S-transferasa.Conclusiones: El perfil molecular de los pacientes sensibilizados al polen del plátano de sombra varía respecto al descrito en estudios deotras localizaciones. Nuestra población muestra una mayor prevalencia de Pla a 2 comparado con Pla a 1 y Pla a 3. Además, el alérgenominoritario previamente denominado Pla a 4 fue caracterizado como una glutatión-S-transferasa. (AU)
Asunto(s)
Humanos , Masculino , Femenino , Niño , Adolescente , Adulto Joven , Adulto , Persona de Mediana Edad , Anciano , Anciano de 80 o más Años , Antígenos de Plantas/análisis , Hipersensibilidad/diagnóstico , Magnoliopsida/inmunología , Polen/inmunología , Alérgenos/inmunología , Hipersensibilidad/epidemiología , Prevalencia , España/epidemiología , Hipersensibilidad Inmediata , Inmunoglobulina E/inmunología , Electroforesis en Gel de Poliacrilamida , Ensayo de Inmunoadsorción EnzimáticaRESUMEN
Plant innate immunity relies on nucleotide binding leucine-rich repeat receptors (NLRs) that recognize pathogen-derived molecules and activate downstream signaling pathways. We analyzed the variation in NLR gene copy number and identified plants with a low number of NLR genes relative to sister species. We specifically focused on four plants from two distinct lineages, one monocot lineage (Alismatales) and one eudicot lineage (Lentibulariaceae). In these lineages, the loss of NLR genes coincides with loss of the well-known downstream immune signaling complex ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1)/PHYTOALEXIN DEFICIENT 4 (PAD4). We expanded our analysis across whole proteomes and found that other characterized immune genes were absent only in Lentibulariaceae and Alismatales. Additionally, we identified genes of unknown function that were convergently lost together with EDS1/PAD4 in five plant species. Gene expression analyses in Arabidopsis (Arabidopsis thaliana) and Oryza sativa revealed that several homologs of the candidates are differentially expressed during pathogen infection, drought, and abscisic acid treatment. Our analysis provides evolutionary evidence for the rewiring of plant immunity in some plant lineages, as well as the coevolution of the EDS1/PAD4 pathway and drought responses.
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Alismatales/genética , Proteínas NLR/genética , Inmunidad de la Planta/genética , Proteínas de Plantas/genética , Alismatales/inmunología , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Hidrolasas de Éster Carboxílico/genética , Proteínas de Unión al ADN/genética , Resistencia a la Enfermedad/genética , Resistencia a la Enfermedad/inmunología , Sequías , Evolución Molecular , Dosificación de Gen , Regulación de la Expresión Génica de las Plantas , Magnoliopsida/genética , Magnoliopsida/inmunología , Oryza/genética , Filogenia , Transducción de Señal , SinteníaRESUMEN
BACKGROUND: The prevalence of allergic rhinitis (AR) is increasing rapidly in Central China. The skin prick test (SPT) is the standard tool with which to determine the allergen sensitization status in AR patients. Changes in allergen sensitization patterns have been observed within countries and regions due to geographical and seasonal variations. OBJECTIVE: The aim of this study was to evaluate the profile of SPT reactivity to different aeroallergens in AR patients and to suggest a minimal panel of allergens to detect sensitized patients in Central China. METHODS: From January 2015 to December 2016, patients who presented to Tongji Hospital with suspected AR were tested with the same panel of 19 aeroallergens. The results of SPT were analyzed to determine the minimum test battery panel necessary to cover 99% of the cases of SPT sensitization in different age subgroups. RESULTS: A total of 2,416 patients (male:female ratio 1.2:1) were enrolled in our study with an average age of 22.0 years. The overall rate of sensitization to any allergen was 79.0%, and 64.3% of the subjects were monosensitized. The highest sensitized rate was found in the subgroup aged 14-18 years (92.0%), followed by the subgroups of 6-14 years (86.4%), >18 years (75.6%), and ≤6 years (74.9%). The most common sensitization was to Dermatophagoides farinae (71.1%). Testing with 8 allergens (D. pteronyssinus, D. farinae, Platanus, Artemisia, Cryptomeria, Blatella germanica, Humulus, and Alternaria) was sufficient to identify over 99% of the sensitized patients. CONCLUSION: An SPT panel covering 8 allergen extracts was able to detect almost all sensitized patients suffering from AR symptoms in Central China.
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Alérgenos/inmunología , Rinitis Alérgica/diagnóstico , Pruebas Cutáneas , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Contaminantes Atmosféricos/inmunología , Alternaria/inmunología , Animales , Antígenos Dermatofagoides/inmunología , Antígenos Fúngicos/inmunología , Antígenos de Plantas/inmunología , Niño , Preescolar , China , Cucarachas/inmunología , Dermatophagoides farinae/inmunología , Dermatophagoides pteronyssinus/inmunología , Femenino , Humanos , Lactante , Proteínas de Insectos/inmunología , Magnoliopsida/inmunología , Masculino , Persona de Mediana Edad , Rinitis Alérgica/inmunología , Adulto JovenRESUMEN
Platanus acerifolia pollen is considered an important source of airborne allergens in numerous cities. Pla a 1 is a major allergen from P. acerifolia pollen. The present study aimed to express and purify Pla a 1, and to prepare its monoclonal antibody. In the present study, the Pla a 1 gene was subcloned into a pET28a vector and transformed into the ArcticExpress™ (DE3) RP Escherichia coli host strain. The purified Pla a 1 was then used to immunize BALB/c mice. When serum detection was positive, spleen cells were isolated from the mice and fused with SP2/0 myeloma cells at a ratio of 10:1. Hybridoma cells were screened by indirect ELISA and limiting dilution. Positive cells were used to induce the formation of antibodycontaining ascites fluid, and the antibodies were purified using protein Aagarose. The results of the present study demonstrated that recombinant Pla a 1 was successfully expressed and purified, and exhibited positive immunoglobulin Ebinding to serum from patients allergic to P. acerifolia. A total of 11 hybridomas that steadily secreted antiPla a 1 antibody were obtained and an immunoblotting analysis indicated that all of these monoclonal antibodies specifically recognized the Pla a 1 protein. These results suggested that specific antiPla a 1 antibodies may be obtained, which can be used for the rapid detection of Pla a 1 allergens and in the preparation of vaccines against P. acerifolia pollen.
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Anticuerpos Monoclonales/inmunología , Antígenos de Plantas/genética , Antígenos de Plantas/inmunología , Clonación Molecular/métodos , Adolescente , Adulto , Animales , Antígenos de Plantas/aislamiento & purificación , Línea Celular , Escherichia coli/genética , Femenino , Vectores Genéticos/genética , Humanos , Inmunoglobulina E/inmunología , Magnoliopsida/genética , Magnoliopsida/inmunología , Masculino , Ratones , Ratones Endogámicos BALB C , Polen , Proteínas Recombinantes/genética , Proteínas Recombinantes/inmunología , Proteínas Recombinantes/aislamiento & purificación , Rinitis Alérgica/inmunología , Rinitis Alérgica Estacional/inmunología , Adulto JovenRESUMEN
Platanus acerifolia (P. acerifolia) is an important cause of pollinosis in cities. The use of allergen extracts on patients with allergic diseases is the most commonly applied method to attempt to treat pollinosis. Pla a 3, a nonspecific lipid transfer protein, is a major allergen present in P. acerifolia pollen extracts. In the present study, the Pla a 3 gene was subcloned into a pSUMOMut vector using Stu I and Xho I sites and transformed into the Arctic Express™ (DE3) RP E. coli host strain. The purified Pla a 3 allergen was analyzed by western blotting and the results revealed that the Pla a 3 allergen has the ability to bind IgE in the P. acerifolia pollen of allergic patients' sera. Moreover, the authors predicted the potential B cell epitopes of the Pla a 3 allergen using the DNAStar Protean system, the Bioinformatics Predicted Antigenic Peptides system and the BepiPred 1.0 server. In addition, the T cell epitopes were predicted by the SYFPEITHI database and the NetMHCII2.2 server. As a result, two B cell epitopes (3545 and 8186) and four potential T cell epitopes including 215, 4550, 5561 and 6773 were predicted in the present study. The current results can be used to contribute to allergen immunotherapies and useful in peptidebased vaccine designs of pollen allergy.
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Epítopos de Linfocito B/inmunología , Epítopos de Linfocito T/inmunología , Inmunoglobulina E/inmunología , Magnoliopsida/inmunología , Rinitis Alérgica Estacional/inmunología , Adolescente , Adulto , Antígenos de Plantas/química , Antígenos de Plantas/genética , Antígenos de Plantas/inmunología , Antígenos de Plantas/aislamiento & purificación , Clonación Molecular , Epítopos de Linfocito B/química , Epítopos de Linfocito B/genética , Epítopos de Linfocito B/aislamiento & purificación , Epítopos de Linfocito T/química , Epítopos de Linfocito T/genética , Epítopos de Linfocito T/aislamiento & purificación , Escherichia coli/genética , Femenino , Humanos , Inmunoglobulina E/sangre , Magnoliopsida/química , Magnoliopsida/genética , Masculino , Persona de Mediana Edad , Modelos Moleculares , Polen/química , Polen/genética , Polen/inmunología , Conformación Proteica , Rinitis Alérgica Estacional/sangre , Adulto JovenRESUMEN
Objective To identify the effect and mechanism of recombinant platanus pollen allergen 2 (rPla a2) on the transcription and expression of orosomucoid 1-like 3 (ORMDL3) gene in NIH3T3 cells. Methods Quantitative real-time PCR and Western blot analysis were used to detect the influence of rPla a2 on the mRNA and protein levels of ORMDL3 and GATA3. After transfection of GATA3 siRNA or pcDNA-GATA3, the role of GATA3 was evaluated in rPla a2 regulating ORMDL3 expression. Dual-luciferase reporter assay was performed to measure the influence of rPla a2 and GATA3 on ORMDL3 promoter activity. Results The rPla a2 induced mRNA and protein expressions of ORMDL3 and GATA3. Knockdown of GATA3 inhibited the induction of rPla a2 upon ORMDL3. In addition, the induction effect was enhanced by over-expression of GATA3. ORMDL3 promoter activity was significantly promoted by rPla a2 and inhibited by knockdown of GATA3. Conclusion The rPla a2 up-regulates the expression of ORMDL3 mRNA and protein, which is mediated by GATA3 through targeting promoter region of ORMDL3.
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Alérgenos/inmunología , Factor de Transcripción GATA3/inmunología , Magnoliopsida/inmunología , Proteínas de la Membrana/inmunología , Polen/inmunología , Proteínas Recombinantes/inmunología , Regulación hacia Arriba/inmunología , Animales , Línea Celular , Ratones , Células 3T3 NIH , Rinitis Alérgica Estacional/inmunologíaRESUMEN
We review the literature on host resistance of ash to emerald ash borer (EAB, Agrilus planipennis), an invasive species that causes widespread mortality of ash. Manchurian ash (Fraxinus mandshurica), which coevolved with EAB, is more resistant than evolutionarily naïve North American and European congeners. Manchurian ash was less preferred for adult feeding and oviposition than susceptible hosts, more resistant to larval feeding, had higher constitutive concentrations of bark lignans, coumarins, proline, tyramine and defensive proteins, and was characterized by faster oxidation of phenolics. Consistent with EAB being a secondary colonizer of coevolved hosts, drought stress decreased the resistance of Manchurian ash, but had no effect on constitutive bark phenolics, suggesting that they do not contribute to increased susceptibility in response to drought stress. The induced resistance of North American species to EAB in response to the exogenous application of methyl jasmonate was associated with increased bark concentrations of verbascoside, lignin and/or trypsin inhibitors, which decreased larval survival and/or growth in bioassays. This finding suggests that these inherently susceptible species possess latent defenses that are not induced naturally by larval colonization, perhaps because they fail to recognize larval cues or respond quickly enough. Finally, we propose future research directions that would address some critical knowledge gaps.
Asunto(s)
Escarabajos/fisiología , Fraxinus/inmunología , Magnoliopsida/inmunología , Reguladores del Crecimiento de las Plantas/farmacología , Acetatos/farmacología , Animales , Antiinfecciosos/metabolismo , Antibiosis , Ciclopentanos/farmacología , Fraxinus/parasitología , Glucósidos/metabolismo , Larva , Lignina/metabolismo , Magnoliopsida/parasitología , Modelos Biológicos , Oviposición , Oxilipinas/farmacología , Fenoles/metabolismo , Especificidad de la Especie , Inhibidores de Tripsina/metabolismo , MaderaRESUMEN
The plant hormone abscisic acid (ABA) regulates many key processes involved in plant development and adaptation to biotic and abiotic stresses. Under stress conditions, plants synthesize ABA in various organs and initiate defense mechanisms, such as the regulation of stomatal aperture and expression of defense-related genes conferring resistance to environmental stresses. The regulation of stomatal opening and closure is important to pathogen defense and control of transpirational water loss. Recent studies using a combination of approaches, including genetics, physiology, and molecular biology, have contributed considerably to our understanding of ABA signal transduction. A number of proteins associated with ABA signaling and responses--especially ABA receptors--have been identified. ABA signal transduction initiates signal perception by ABA receptors and transfer via downstream proteins, including protein kinases and phosphatases. In the present review, we focus on the function of ABA in stomatal defense against biotic and abiotic stresses, through analysis of each ABA signal component and the relationships of these components in the complex network of interactions. In particular, two ABA signal pathway models in response to biotic and abiotic stress were proposed, from stress signaling to stomatal closure, involving the pyrabactin resistance (PYR)/PYR-like (PYL) or regulatory component of ABA receptor (RCAR) family proteins, 2C-type protein phosphatases, and SnRK2-type protein kinases.
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Ácido Abscísico/metabolismo , Sequías , Magnoliopsida/metabolismo , Estomas de Plantas/fisiología , Transducción de Señal , Estrés Fisiológico , Magnoliopsida/inmunología , Magnoliopsida/fisiología , Estomas de Plantas/metabolismoRESUMEN
BACKGROUND: For the etiological diagnosis of allergic respiratory diseases skin tests or specific serum IgE determination are used. OBJECTIVE: To determine the correlation between the extent of reactivity to cutaneous prick tests and the levels of pollen specific serum IgE in patients with respiratory allergy. PATIENTS AND METHODS: A prolective, descriptive and transversal study was done with patients of both genders, aged 2 to 60 years, who attended for the first time at the service of Allergy and Clinical Immunology of University Hospital of Puebla, Mexico, with presumptive diagnosis of respiratory allergy. All patients underwent clinical history, skin prick tests with standardized allergenic extracts and quantification of pollen specific serum IgE by chemiluminescence method. We estimated the correlation index r using the statistical method Pearson product-moment correlation coefficient; a value r equal to or higher than 0.70 was considered a significant relationship or a high correlation. RESULTS: Nine-one patients were included, of whom 58.2% were female. The diagnoses were: allergic rhinitis (79.1%), asthma and allergic rhinitis (16.5%) and only asthma (4.4%). Only significant correlation was found in patients with allergic rhinitis for Rumex crispus (r = 0.702) and in patients with asthma and rhinitis for Ambrosia trifida (r = 1). CONCLUSION: Only for Rumex crispus and Ambrosia trifida, the skin prick tests or the determination of specific serum IgE levels are comparable diagnostic methods of allergic respiratory diseases.
ANTECEDENTES: para el diagnóstico etiológico de las enfermedades alérgicas respiratorias se realizan pruebas cutáneas o determinación de IgE sérica específica. OBJETIVO: determinar la correlación entre la magnitud de la reactividad a las pruebas cutáneas por punción y las concentraciones de IgE séricaespecífica para pólenes en pacientes con alergia respiratoria. PACIENTES Y MÉTODOS: estudio prolectivo, descriptivo y transversal efectuado en pacientes de uno y otro sexo, con edades de 2 a 60 años, que acudieron por primera vez al servicio de Alergia e Inmunología Clínica del Hospital Universitario de Puebla con diagnóstico presuntivo de alergia respiratoria. A todos los pacientes se les realizó historia clínica, pruebas cutáneas por punción con extractos alergénicos estandarizados y cuantificación de IgE sérica específica para pólenes por método de quimioluminiscencia. Se estimó el índice de correlación r con el método estadístico coeficiente de correlación producto-momento de Pearson, un valor de r igual o mayor de 0.70 se consideró una relación notable o una correlación elevada. RESULTADOS: se incluyeron 91 pacientes, 58.2% eran mujeres. Los diagnósticos fueron: rinitis alérgica (79.1%), asma y rinitis (16.5%) y únicamente asma (4.4%). Sólo se encontró una correlación significativa en pacientes con rinitis alérgica para Rumex crispus (r = 0.702) y en pacientes con asma y rinitis para Ambrosia trifida (r = 1). CONCLUSIÓN: sólo para Rumex crispus y Ambrosia trifida, las pruebas epicutáneas o la determinación de las concentraciones de IgE sérica específica son métodos equiparables en el diagnóstico etiológico de la alérgica respiratoria.
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Alérgenos/inmunología , Asma/inmunología , Inmunoglobulina E/sangre , Pruebas Intradérmicas , Polen/inmunología , Rinitis Alérgica Estacional/inmunología , Adolescente , Adulto , Especificidad de Anticuerpos , Asma/sangre , Asma/diagnóstico , Niño , Preescolar , Estudios Transversales , Femenino , Humanos , Mediciones Luminiscentes , Magnoliopsida/inmunología , Masculino , Persona de Mediana Edad , Pinus/inmunología , Estudios Prospectivos , Rinitis Alérgica Estacional/sangre , Rinitis Alérgica Estacional/diagnóstico , Especificidad de la EspecieRESUMEN
Platanus hispanica pollen is considered an important source of aeroallergens in many Southern European cities. This tree is frequently used in urban green spaces as ornamental specie. The flowering period is greatly influenced by the meteorological conditions, which directly affect its allergenic load in the atmosphere. The purpose of this study is to develop equations to predict the Platanus allergy risk periods as a function of the airborne pollen, the allergen concentration and the main meteorological parameters. The study was conducted by means two volumetric pollen samplers; a Lanzoni VPPS 2000 for the Platanus pollen sampling and a Burkard multivial Cyclone Sampler to collect the aeroallergen particles (Pla a_1). In addiction the Dot-Blot and the Raman spectroscopy methods were used to corroborate the results. The Pla a_1 protein is recorded in the atmosphere after the presence of the Platanus pollen, which extend the Platanus pollen allergy risk periods. The Platanus pollen and the Pla a 1 allergens concentration are associated with statistical significant variations of some meteorological variables: in a positive way with the mean and maximum temperature whereas the sign of the correlation coefficient is negative with the relative humidity. The lineal regression equation elaborated in order to forecast the Platanus pollen content in the air explain the 64.5% of variance of the pollen presence in the environment, whereas the lineal regression equation elaborated in order to forecast the aeroallergen a 54.1% of the Pla a_1 presence variance. The combination of pollen count and the allergen quantification must be assessed in the epidemiologic study of allergic respiratory diseases to prevent the allergy risk periods.
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Alérgenos/inmunología , Polen/inmunología , Atmósfera , Ensayo de Inmunoadsorción Enzimática , Immunoblotting/métodos , Magnoliopsida/inmunología , Espectrometría Raman , ÁrbolesRESUMEN
Although plants possess an arsenal of constitutive defences such as structural barriers and preformed antimicrobial defences, many attackers are able to overcome the pre-existing defence layers. In response, a range of inducible plant defences is set up to battle these pathogens. These mechanisms, commonly integrated as induced resistance (IR), control pathogens and pests by the activation of specific defence pathways. IR mechanisms have been extensively studied in the Dicotyledoneae, whereas knowledge of IR in monocotyledonous plants, including the globally important graminaceous crop plants, is elusive. Considering the potential of IR for sustainable agriculture and the recent advances in monocot genomics and biotechnology, IR in monocots is an emerging research field. In the following, current facts and trends concerning basal immunity, and systemic acquired/induced systemic resistance in the defence of monocots against pathogens and herbivores will be summarized.
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Resistencia a la Enfermedad/inmunología , Magnoliopsida/inmunología , Animales , Herbivoria/fisiología , Enfermedades de las Plantas/inmunología , Inmunidad de la Planta , Receptores de Reconocimiento de Patrones/metabolismoRESUMEN
A patient monosensitized to plane pollen is described On ISAC 103 the patients showed IgE reactivity to both Pla a 1 and Pla a 2, but on immunoblot analysis the serum showed a single IgE reactivity at about 50 kDa allergen.
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Alérgenos/inmunología , Magnoliopsida/inmunología , Polen/inmunología , Adulto , Antígenos de Plantas , Humanos , Inmunoglobulina E/sangre , MasculinoRESUMEN
In plants, the genomic clustering of non-homologous genes for the biosynthesis of chemical defense compounds is an emerging theme. Gene clustering is also observed for polymorphic sexual traits under balancing selection, and examples in plants are self-incompatibility and floral dimorphy. The chemical defense pathways organized as gene clusters are self-contained biosynthetic modules under opposing selection pressures and adaptive polymorphisms, often the presence or absence of a functional pathway, are observed in nature. We propose that these antagonistic selection pressures favor closer physical linkage between beneficially interacting alleles as the resulting reduction in recombination maintains a larger fraction of the fitter genotypes. Gene clusters promote the stable inheritance of functional chemical defense pathways in the dynamic ecological context of natural populations.
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Genes de Plantas , Magnoliopsida/genética , Familia de Multigenes , Proteínas de Plantas/biosíntesis , Vías Biosintéticas/genética , Herbivoria , Magnoliopsida/inmunología , Magnoliopsida/metabolismo , Enfermedades de las Plantas/inmunología , Polimorfismo Genético , Selección GenéticaRESUMEN
BACKGROUND: In the temperate climate zone of the Northern hemisphere, Fagales pollen allergy represents the main cause of winter/spring pollinosis. Among Fagales trees, pollen allergies are strongly associated within the Betulaceae and the Fagaceae families. It is widely accepted that Fagales pollen allergies are initiated by sensitization against Bet v 1, the birch pollen major allergen, although evidence is accumulating that the allergenic activity of some Bet v 1-like molecules has been underestimated. OBJECTIVE: To investigate the allergenic potential of the clinically most important Fagales pollen allergens from birch, alder, hazel, hornbeam, hop-hornbeam, oak, beech and chestnut. METHODS: To obtain the full spectrum of allergens, the three previously unavailable members of the Bet v 1-family, hop-hornbeam Ost c 1, chestnut Cas s 1 and beech Fag s 1, were identified in the respective pollen extracts, cloned and produced as recombinant proteins in E. coli. Together with recombinant Bet v 1, Aln g 1, Car b 1, Cor a 1 and Que a 1, the molecules were characterized physicochemically, mediator release assays were performed and IgE cross-reactivity was evaluated by ELISA and Immuno Solid-phase Allergen Chip (ISAC) IgE inhibition assays. RESULTS: All allergens showed the typical Bet v 1-like secondary structure elements, and they were all able to bind serum IgE from Fagales allergic donors. Strong IgE binding was observed for Betuloideae and Coryloideae allergens, however, cross-reactivity between the two subfamilies was limited as explored by inhibition experiments. In contrast, IgE binding to members of the Fagaceae could be strongly inhibited by serum pre-incubation with allergens of the Betuloideae subfamily. CONCLUSIONS AND CLINICAL RELEVANCE: The data suggest that Bet v 1-like allergens of the Betuloideae and Coryloideae subfamily might have the potential to induce IgE antibodies with different specificities, while allergic reactions towards Fagaceae allergens are the result of IgE cross-reactivity.
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Antígenos de Plantas/inmunología , Hipersensibilidad Inmediata/inmunología , Magnoliopsida/inmunología , Polen/inmunología , Adolescente , Adulto , Anciano , Secuencia de Aminoácidos , Antígenos de Plantas/química , Antígenos de Plantas/genética , Niño , Reacciones Cruzadas/inmunología , Femenino , Humanos , Inmunoglobulina E/sangre , Inmunoglobulina E/inmunología , Inmunoglobulina E/metabolismo , Masculino , Persona de Mediana Edad , Datos de Secuencia Molecular , Polen/metabolismo , Unión Proteica/inmunología , Estructura Secundaria de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/inmunología , Alineación de Secuencia , Adulto JovenRESUMEN
Intervessel pits act as safety valves that prevent the spread of xylem embolism. Pectin-calcium crosslinks within the pit membrane have been proposed to affect xylem vulnerability to cavitation. However, as the chemical composition of pit membranes is poorly understood, this hypothesis has not been verified. Using electron microscopy, immunolabeling, an antimonate precipitation technique, and ruthenium red staining, we studied the distribution of selected polysaccharides and calcium in the pit membranes of four angiosperm tree species. We tested whether shifts in xylem vulnerability resulting from perfusion of stems with a calcium chelating agent corresponded with the distribution of pectic homogalacturonans (HG) and/or calcium within interconduit pit membranes. No HG were detected in the main part of intervessel pit membranes, but were consistently found in the marginal membrane region known as the annulus. Calcium colocalized with HG in the annulus. In contrast to intervessel pits, the membrane of vessel-ray pits showed a high pectin content. The presence of two distinct chemical domains, the annulus and the actual pit membrane, can have substantial implications for pit membrane functioning. We propose that the annulus could affect the observed shift in xylem vulnerability after calcium removal by allowing increased pit membrane deflection.
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Calcio/metabolismo , Epítopos/inmunología , Magnoliopsida/inmunología , Pectinas/inmunología , Xilema/inmunología , Especificidad de Anticuerpos/inmunología , Esterificación , Glucanos/inmunología , Magnoliopsida/metabolismo , Magnoliopsida/ultraestructura , Metilación , Rojo de Rutenio/metabolismo , Especificidad de la Especie , Coloración y Etiquetado , Xilanos/inmunología , Xilema/metabolismo , Xilema/ultraestructuraRESUMEN
The IAN (immune-associated nucleotide-binding protein) family belongs to a family of AIG1-like GTPases. These functionally uncharacterized GTP-binding proteins have unique structures and are differentially expressed in both vertebrate immune cells and plant cells during antibacterial responses. In mammals, the IANs, as a novel family of T cell receptor-responsive proteins, play critical roles in regulation of thymic development and survival of T lymphocytes through the interaction with Bcl-2 family proteins. The Arabidopsis AIG1 and AIG2, which are first identified IAN proteins, are involved in plant resistance to bacteria. Recent analysis of the expression patterns of Arabidopsis IANs suggests that these IAN proteins may play regulatory roles during plant development and response to both biotic and abiotic stress.
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Proteínas de Unión al GTP/genética , Magnoliopsida/genética , Familia de Multigenes , Vertebrados/genética , Animales , Apoptosis , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/inmunología , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/genética , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/inmunología , Proteínas de Unión al GTP/inmunología , Magnoliopsida/inmunología , Mitocondrias/metabolismo , Linfocitos T/inmunología , Vertebrados/inmunologíaRESUMEN
A total of 65 pollen types were identified from two years atmospheric pollen survey in the environmental conditions of South Assam. Out of them, eight pollen types viz., Acacia auriculiformis, Amaranthus spinosus, Cassia alata, Cleome gynandra, Cocos nucifera, Imperata cylindrica, Ricinus communis and Trewia nudiflora, were selected for biochemical studies on the basis of their dominance in the study sites. Among the sample extract tested, Ricinus communis was found to contain the highest amount of soluble protein, free amino acid and total carbohydrate, per gram of dry weight followed by Imperata cylindrica and Cassia alata. Maximum numbers of protein polypeptide bands were detected in the sample extract of Cassia alata by polyacrylamide gel electrophoresis method followed by Acacia auriculiformis, Imperata cylindrica and Cocos nucifera. IgE binding protein fractions were maximum in Cassia alata and minimum in Trewia nudiflora.
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Magnoliopsida/química , Magnoliopsida/inmunología , Proteínas de Plantas/química , Proteínas de Plantas/inmunología , Polen/química , Polen/inmunología , Femenino , Humanos , Inmunoglobulina E/química , Inmunoglobulina E/inmunología , India , Masculino , Rinitis Alérgica Estacional/inmunología , Especificidad de la EspecieRESUMEN
The radioprotective effect of Biophytum sensitivum methanol extract was studied using in vivo mice model . Animals were exposed to whole body gamma irradiation (6 Gy/animal) after treatment with B. sensitivum (50mg/kg b.wt.) followed by estimation of cytosolic enzymes, level of antioxidants, hematological parameters, bone marrow cellular progenitors, serum cytokine levels and spleen hematopoietic colonies. Administration of B. sensitivum could reduce the enhanced level of ALP, GPT and LPO levels in irradiated animals. B. sensitivum could significantly enhance the glutathione (GSH) content in liver and intestinal mucosa of irradiated animals. B. sensitivum treatment could enhance the Total WBC count, cellularity of bone marrow, alpha-esterase positive cells, and relative organ weight of spleen as well as thymus. The number of hematopoietic colonies on the surface of the spleen was found to be enhanced after B. sensitivum treatment. B. sensitivum treatment could also stimulate the production of cytokines such as IL-1beta, IFN-gamma and GM-CSF in animals exposed to whole body gamma irradiation. The present investigation suggests that the protective effect of Biophytum sensitivum on Radiation-Induced hemopoietic damage is mediated through immunomodulation as well as sequential induction of IL-1beta, GM-CSF and IFN-gamma.