RESUMEN
Isolation of viable immune cells from human tissues is critical for the characterization of cellular and molecular processes underlying disease pathogenesis. Here, we describe protocols for the isolation of highly viable immune cells from liver wedges and mesenteric white adipose tissue resections from obese persons. Notably, characterization of the isolated single-immune cell suspensions, via utility of basic immunological interrogations and genetic approaches, promises to generate an improved understanding of altered immunological pathways in obese individuals with or without metabolic diseases. For complete details on the use and execution of this protocol, please refer to Moreno-Fernandez et al. (2021).
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Separación Celular/métodos , Sistema Inmunológico/citología , Hígado , Mesenterio , Análisis de la Célula Individual/métodos , Tejido Adiposo Blanco/citología , Tejido Adiposo Blanco/patología , Adolescente , Biopsia , Células Cultivadas , Femenino , Humanos , Hígado/citología , Hígado/patología , Masculino , Mesenterio/citología , Mesenterio/patología , Obesidad InfantilRESUMEN
Lymphatic muscle cells (LMCs), with unique characteristics resembling a combination of both cardiac and smooth muscle cells, play an essential role in the spontaneous contraction of the lymphatic vessels to pump fluid forward. However, our understanding of the more detailed molecular phenotypes of LMCs is limited. Here, we described a method to isolate the LMCs from rat mesentery and then culture the cells in vitro, which will serve a lot more molecular biology study of LMCs and significantly improve our knowledge about the unique characteristics of LMCs.
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Técnicas de Cultivo de Célula/métodos , Separación Celular/métodos , Disección/métodos , Mesenterio/citología , Células Musculares/citología , Animales , Técnica del Anticuerpo Fluorescente , Células Musculares/metabolismo , RatasRESUMEN
Pathological alterations of lymphatic structure and function interfere with lymph transport, resulting in a wide range of clinical disorders that include edema, tissue inflammation, and metabolic syndromes. Mesentery contains abundant lymphatic vessels and plays an important role in transporting absorbed lipid from the intestine. In this manuscript, we describe a whole-mount staining method on isolated mouse mesentery with VEGFR3, Prox1, and Lyve1 antibodies to visualize the morphology of lymphatic vessels.
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Linfangiogénesis , Vasos Linfáticos/metabolismo , Mesenterio/citología , Microscopía Fluorescente/métodos , Coloración y Etiquetado/métodos , Animales , Embrión de Mamíferos/metabolismo , Femenino , Proteínas de Homeodominio/metabolismo , Mesenterio/metabolismo , Ratones , Proteínas Supresoras de Tumor/metabolismo , Factores de Crecimiento Endotelial Vascular/metabolismo , Proteínas de Transporte Vesicular/metabolismoRESUMEN
INTRODUCTION: Clinical trials are currently investigating whether an extended mesenteric resection for ileocecal resections could reduce postoperative recurrence in Crohn's disease. Resection of the mesorectum, which contains proinflammatory macrophages, during proct(ocol)ectomy, is associated with reduced recurrent inflammation and improved wound healing. We aimed to characterize the macrophages in the ileocecal mesentery, which were compared with those in the mesorectum, to provide a biological rationale for the ongoing trials. METHODS: In 13 patients with Crohn's disease and 4 control patients undergoing a proctectomy, tissue specimens were sampled at 3 locations from the mesorectum: distal (rectum), middle, and proximal (sigmoid). In 38 patients with Crohn's disease and 7 control patients undergoing ileocecal resections, tissue specimens also obtained from 3 locations: adjacent to the inflamed terminal ileum, adjacent to the noninflamed ileal resection margin, and centrally along the ileocolic artery. Immune cells from these tissue specimens were analyzed by flow cytometry for expression of CD206 to determine their inflammatory status. RESULTS: In the mesorectum, a gradient from proinflammatory to regulatory macrophages from distal to proximal was observed, corresponding to the adjacent inflammation of the intestine. By contrast, the ileocecal mesentery did not contain high amounts of proinflammatory macrophages adjacent to the inflamed tissue, and a gradient toward a more proinflammatory phenotype was seen in the central mesenteric area. DISCUSSION: Although the mesentery is a continuous structure, the mesorectum and the ileocecal mesentery show different immunological characteristics. Therefore, currently, there is no basis to perform an extended ileocecal resection in patients with Crohn's disease.
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Colectomía/métodos , Enfermedad de Crohn/cirugía , Macrófagos/inmunología , Mesenterio/citología , Proctectomía/métodos , Adulto , Anciano , Ciego/citología , Ciego/inmunología , Ciego/patología , Ciego/cirugía , Estudios de Cohortes , Colon Sigmoide/citología , Colon Sigmoide/inmunología , Colon Sigmoide/patología , Colon Sigmoide/cirugía , Enfermedad de Crohn/inmunología , Enfermedad de Crohn/patología , Femenino , Humanos , Íleon/citología , Íleon/inmunología , Íleon/patología , Íleon/cirugía , Masculino , Mesenterio/inmunología , Mesenterio/patología , Mesenterio/cirugía , Persona de Mediana Edad , Recto/citología , Recto/inmunología , Recto/patología , Recto/cirugía , Recurrencia , Prevención Secundaria/métodos , Adulto JovenRESUMEN
We studied the effects and mechanisms of action of NaHS, an H2S donor, on isolated phenylephrine-precontracted bovine mesenteric lymph nodes. NaHS induced concentration-dependent relaxation of lymph nodes. Removal of the endothelium reduced, but did not abolish the relaxing effect of NaHS. The relaxing effect was reduced by NO synthase inhibitor L-NAME, soluble guanylate cyclase inhibitor ODQ, ATP-sensitive K+ channel blocker glibenclamide, and a combination charybdotoxin+apamin (blockers of small- and intermediate-conductance Ca2+-activated K+ channels). Thus, the relaxing effect of H2S on lymph nodes is mediated by several parallel mechanisms. H2S induces relaxation of LN and modulates the rate of lymph transport, thereby affecting the development of immune processes in the body.
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Sulfuro de Hidrógeno/farmacología , Ganglios Linfáticos/efectos de los fármacos , Transportadoras de Casetes de Unión a ATP/metabolismo , Animales , Bovinos , GMP Cíclico/metabolismo , Indometacina/farmacología , Canales de Potasio de Gran Conductancia Activados por el Calcio/metabolismo , Mesenterio/citología , NG-Nitroarginina Metil Éster/farmacología , Óxido Nítrico/metabolismoRESUMEN
Lymphoid tissue inducer (LTi)/LTi-like cells are critical for lymphoid organogenesis and regulation of adaptive immunity in various tissues. However, the maintenance and regulation mechanisms of LTi-like cells among different tissues are not clear yet. Here, we find that LTi-like cells from different tissues display heterogeneity. The maintenance of LTi-like cells in the mesenteric lymph node (mLN), but not the gut, requires RANKL signaling from CD4+ T cells. LTi-like cells from the mLN, but not the gut, could in turn inhibit the development of T follicular helper cells and subsequent humoral responses during intestinal immunization in an ID2- and PD-L1-dependent manner. Together, our findings implicate that the interaction between LTi-like cells and T cells in the mLN could precisely control the intestinal mucosal adaptive immune response.
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Inmunidad Humoral , Intestinos/inmunología , Ganglios Linfáticos/citología , Ganglios Linfáticos/inmunología , Mesenterio/citología , Linfocitos T/citología , Linfocitos T/inmunología , Animales , Antígeno B7-H1/metabolismo , Linfocitos T CD4-Positivos/citología , Linfocitos T CD4-Positivos/inmunología , Inmunización , Ratones , Ligando RANK/metabolismo , Receptor Activador del Factor Nuclear kappa-B/metabolismo , Transducción de Señal , Transcripción GenéticaRESUMEN
Metastasis accounts for nearly 90% of all cancer associated mortalities. A hallmark of metastasis in malignancies of epithelial origin such as in the pancreas and breast, is invasion of the basement membrane (BM). While various in vitro assays have been developed to address questions regarding the invasiveness of tumors with relation to the BM, most fail to recapitulate a physiologically accurate cell-membrane interface. Here, we introduce a new 3D in vitro assay that uses the mouse mesenteric tissue as a mimic for the epithelial BM. We describe a simple, cost-effective protocol for extraction and setup of the assay, and show that the mesentery is a physiologically accurate model of the BM in its key components-type IV collagen, laminin-1 and perlecan. Furthermore, we introduce a user-friendly quantification tool, Q-Pi, which allows the 3D reconstruction, visualization and quantification of invasion at a cellular level. Overall, we demonstrate that this invasion assay provides a physiologically accurate tool to investigate BM invasion.
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Membrana Basal/citología , Bioensayo/métodos , Mesenterio/citología , Técnicas de Cultivo de Tejidos/métodos , Animales , Membrana Basal/metabolismo , Movimiento Celular , Células Epiteliales , Epitelio/metabolismo , Proteínas de la Matriz Extracelular/metabolismo , Ratones , Invasividad Neoplásica/patologíaRESUMEN
Immune cells resident in adipose tissue have important functions in local and systemic metabolic homeostasis. Nevertheless, these immune cell populations remain poorly characterized in bovines. Recently, we described diverse lymphocyte subpopulations in adipose tissue of Holstein-Friesian cows. Here, we aimed at characterising myeloid cell populations present in bovine adipose tissue using multicolour flow cytometry, cell sorting and histochemistry/immunohistochemistry. Macrophages, CD14+CD11b+MHC-II+CD45+ cells, were identified in mesenteric and subcutaneous adipose tissue, though at higher proportions in the latter. Mast cells, identified as SSC-AhighCD11b-/+CD14-MHC-II-CH138A-CD45+ cells, were also observed in adipose tissue and found at higher proportions than macrophages in mesenteric adipose tissue. Neutrophils, presenting a CH138A+CD11b+ phenotype, were also detected in mesenteric and subcutaneous adipose tissue, however, at much lower frequencies than in the blood. Our gating strategy allowed identification of eosinophils in blood but not in adipose tissue although being detected by morphological analysis at low frequencies in some animals. A population not expressing CD45 and with the CH138A+ CD11b-MHC-II- phenotype, was found abundant and present at higher proportions in mesenteric than subcutaneous adipose tissue. The work reported here may be useful for further studies addressing the function of the described cells.
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Antígenos CD/metabolismo , Mesenterio/metabolismo , Células Mieloides/metabolismo , Grasa Subcutánea/metabolismo , Animales , Bovinos , Femenino , Citometría de Flujo , Inmunohistoquímica , Mastocitos/citología , Mastocitos/metabolismo , Mesenterio/citología , Células Mieloides/citología , Células Progenitoras Mieloides/citología , Células Progenitoras Mieloides/metabolismo , Grasa Subcutánea/citologíaRESUMEN
Since chemerin's identification as an adipokine, it has been associated with a number of human diseases including diabetes and obesity. However, the basic scientific foundation for these clinical determinations is still lacking. Fibroblastic mouse 3T3 cells are unable to develop lipid droplets if chemerin is not present. Thus, we hypothesized that an in vivo rat model chemerin knockout (KO; an advancement from the previously mentioned in vitro cultures) would have limited accumulation of lipid in adipocytes compared to their wild-type (WT) counterparts. Female WT/KO rats (Sprague Dawley background) were fed a low-fat diet starting at 8 weeks of age with weekly body weight and food consumption monitoring. At 25 weeks of age, adipose tissue depots were dissected and flash frozen for PCR analysis or fixed with paraformaldehyde for histology. Over the 17 weeks of experimentation, WT and KO animals did not have differences in total body weight or food consumption but KO animals had a significantly reduced amount of visceral fat compared to WT animals (via microCT at 8 and 25 weeks). Histology of retroperitoneal and mesenteric depots demonstrated a significant leftward shift in adipocyte size in the mesenteric but not the retroperitoneal depot of the KO compared to WT animals. Similarly, in the mesenteric fat of the KO rat, gene expression of adiponectin, fatty acid synthase, perilipin, and leptin were significantly reduced compared to mesenteric fat of WT animals and retroperitoneal fat of both WT and KO animals. Adiponectin was highlighted by a protein-protein interaction network as being important for the physiological effects of chemerin removal. These data are the first, to our knowledge, to demonstrate chemerin's adipokine potential in vivo and identify it as fat depot location-specific.
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Adipogénesis/genética , Quimiocinas/análisis , Adipocitos/fisiología , Adipoquinas/fisiología , Tejido Adiposo/citología , Animales , Peso Corporal , Quimiocinas/genética , Quimiocinas/fisiología , Dieta con Restricción de Grasas , Técnicas de Inactivación de Genes , Grasa Intraabdominal , Gotas Lipídicas , Mesenterio/citología , Ratas , Ratas Sprague-DawleyRESUMEN
The cellular homeostasis of lymphoid tissues is determined by the continuous interactions of mobile hematopoietic cells within specialized microenvironments created by sessile stromal cells. In contrast to the lymph nodes and mucosal lymphoid tissues with well-defined entry and exit routes, the movement of leukocytes in the peritoneal cavity is largely unknown. In this study, we report that, in addition to the omental milky spots and fat-associated lymphoid clusters, in mice, the serous surface of the mesenteric adipose streaks contains lymphocyte-rich organoids comprised of a highly compacted leaf-like part connected to the adipose tissue that can also efficiently bind B cells and high-grade B cell lymphoma (diffuse large B cell lymphoma) cells. Denoted as foliate lymphoid aggregates (FLAgs), these structures show incomplete T/B segregation and a partially differentiated stromal architecture. LYVE-1-positive macrophages covering FLAgs efficiently bind i.p. injected normal B cells as well as different types of diffuse large B cell lymphoma cells. Within FLAgs, the lymphocytes compartmentalize according to their chemokine receptor pattern and subsequently migrate toward the mesenteric lymph nodes via the mesenteric lymphatic capillaries. The blood supply of FLAgs includes short vascular segments displaying peripheral lymph node addressin, and the extravasation of lymphocytes to the omental and mesenteric adipose tissues is partly mediated by L-selectin. The appearance of i.p. injected cells in mesenteric lymph nodes suggests that the mesentery-associated lymphatics may also collect leukocytes from the fat-associated lymphoid clusters and FLAgs, thus combining the mucosal and serous exit of mobile leukocytes and increasing the range of drainage sites for the peritoneal expansion of lymphoid malignancies.
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Linfocitos B/inmunología , Movimiento Celular/inmunología , Linfoma de Células B Grandes Difuso/patología , Mesenterio/citología , Cavidad Peritoneal/citología , Animales , Línea Celular , Selectina L/metabolismo , Leucocitos/inmunología , Ganglios Linfáticos/citología , Vasos Linfáticos/metabolismo , Linfoma de Células B Grandes Difuso/inmunología , Macrófagos/inmunología , Proteínas de Transporte de Membrana/metabolismo , Mesenterio/metabolismo , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Ratones Transgénicos , Microambiente Tumoral/inmunologíaRESUMEN
The majority of genetically modified C57BL/6 mice contain congenic passenger DNA around the targeted gene locus as they were generated from 129-derived embryonic stem cells (ESCs) with subsequent backcrossing to the C57BL/6 genetic background. When studying the role of atypical chemokine receptor 4 (ACKR4) in the immune system, we realized that the two available Ackr4-deficient mouse strains (Ackr4-/- and Ackr4GFP/GFP ) show profoundly different phenotypes: Compared to wild-type and Ackr4GFP/GFP mice, Ackr4-/- mice show a strong accumulation of plasma blasts in mesenteric lymph node and spleen as well as increased B cell proliferation after in vitro activation. This phenotype was maintained after further backcrossing to C57BL/6 mice and was even present in heterozygous Ackr4+/- animals, suggesting that a gene variant on the targeted chromosome might cause this phenotype. Exome sequencing revealed that a region of approximately 20 Mbp around the Ackr4 locus on chromosome 9 still originates from the 129 background based on high variant density observed. In activated Ackr4-/- and Ackr4GFP/GFP B cells, transcripts of genes around the Ackr4 locus were equally deregulated compared to C57BL/6 B cells, whereas increased expression of IL-6 was selectively observed in B cells of Ackr4-/- mice. Because the gene encoding for IL-6 is placed on chromosome 5 these findings suggest that passenger DNA around the Ackr4 locus has an indirect effect on B cell activation and IL-6 production. Results of the present study should not only lead to the reinterpretation of data from earlier studies using Ackr4-/- mice but should remind the scientific community about the limitations of mouse models using mice created by gene-targeting of nonsyngeneic ESCs.
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Linfocitos B/inmunología , Cromosomas de los Mamíferos/inmunología , Interleucina-6/genética , Ganglios Linfáticos/inmunología , Activación de Linfocitos , Receptores CCR/genética , Animales , Linfocitos B/citología , Proliferación Celular , Cruzamientos Genéticos , Células Madre Embrionarias/citología , Células Madre Embrionarias/inmunología , Femenino , Genes Reporteros , Sitios Genéticos , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Heterocigoto , Homocigoto , Interleucina-6/inmunología , Ganglios Linfáticos/citología , Masculino , Mesenterio/citología , Mesenterio/inmunología , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Fenotipo , Receptores CCR/deficiencia , Receptores CCR/inmunología , Bazo/citología , Bazo/inmunología , Secuenciación del ExomaRESUMEN
Redundant mechanisms support immunoglobulin A (IgA) responses to intestinal antigens. These include multiple priming sites [mesenteric lymph nodes (MLNs), Peyer's patches, and isolated lymphoid follicles] and various cytokines that promote class switch to IgA, even in the absence of T cells. Despite these backup mechanisms, vaccination against enteric pathogens such as rotavirus has limited success in some populations. Genetic and environmental signals experienced during early life are known to influence mucosal immunity, yet the mechanisms for how these exposures operate remain unclear. Here, we used rotavirus infection to follow antigen-specific IgA responses through time and in different gut compartments. Using genetic and pharmacological approaches, we tested the role of the lymphotoxin (LT) pathway-known to support IgA responses-at different developmental stages. We found that LT-ß receptor (LTßR) signaling in early life programs intestinal IgA responses in adulthood by affecting antibody class switch recombination to IgA and subsequent generation of IgA antibody-secreting cells within an intact MLN. In addition, early-life LTßR signaling dictates the phenotype and function of MLN stromal cells to support IgA responses in the adult. Collectively, our studies uncover new mechanistic insights into how early-life LTßR signaling affects mucosal immune responses during adulthood.
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Inmunoglobulina A/inmunología , Ganglios Linfáticos/inmunología , Receptor beta de Linfotoxina/inmunología , Linfotoxina-alfa/inmunología , Mesenterio/inmunología , Células del Estroma/inmunología , Animales , Heces/microbiología , Femenino , Inmunidad Mucosa , Ganglios Linfáticos/citología , Receptor beta de Linfotoxina/genética , Linfotoxina-alfa/genética , Masculino , Mesenterio/citología , Ratones Endogámicos C57BL , Ratones NoqueadosRESUMEN
Galectin-3 (Gal-3) controls intercellular and cell-extracellular matrix interactions during immunological responses. In chronic inflammation, Gal-3 is associated with fibrotic events, regulates B cell differentiation and delays lupus progression. Gal-3 deficient mice (Lgals3-/-) have intense germinal center formation and atypical plasma cell generation correlated to high levels IgG, IgE, and IgA. Here, we used pristane (2,6,10,14-tetramethylpentadecane) to induce lupus-like syndrome in Lgals3-/- and Lgals3+/+ BALB/c mice. Mesentery and peritoneal cells were monitored because promptly react to pristane injected in the peritoneal cavity. For the first time, mesenteric tissues have been associated to the pathogenesis of experimental lupus-like syndrome. In Lgals3+/+ pristane-induced mice, mesentery was hallmarked by intense fibrogranulomatous reaction restricted to submesothelial regions and organized niches containing macrophages and B lymphocytes and plasma cells. In contrast, Lgals3-/- pristane-treated mice had diffuse mesenteric fibrosis affecting submesothelium and peripheral tissues, atypical M1/M2 macrophage polarization and significant DLL1+ cells expansion, suggesting possible involvement of Notch/Delta pathways in the disease. Early inflammatory reaction to pristane was characterized by significant disturbances on monocyte recruitment, macrophage differentiation and dendritic cell (DC) responses in the peritoneal cavity of pristane-induced Lgals3-/- mice. A correlative analysis showed that mesenteric damages in the absence of Gal-3 were directly associated with severe portal inflammation and hepatitis. In conclusion, it has suggested that Gal-3 orchestrates histological organization in the mesentery and prevents lupoid hepatitis in experimental lupus-like syndrome by controlling macrophage polarization, Notch signaling pathways and DC differentiation in mesenteric structures.
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Galectina 3/metabolismo , Hepatitis/inmunología , Lupus Eritematoso Sistémico/inmunología , Macrófagos Peritoneales/inmunología , Mesenterio/patología , Animales , Modelos Animales de Enfermedad , Femenino , Fibrosis , Galectina 3/genética , Hepatitis/patología , Humanos , Inyecciones Intraperitoneales , Hígado/inmunología , Hígado/patología , Lupus Eritematoso Sistémico/inducido químicamente , Lupus Eritematoso Sistémico/complicaciones , Mesenterio/citología , Mesenterio/inmunología , Ratones , Ratones Noqueados , Terpenos/administración & dosificación , Terpenos/inmunologíaRESUMEN
Adipose tissue (AT) has wide functions as an active endocrine organ acting as a site of nutrient storage and thermogenesis. Recently it has been identified as having a key role in murine and human immunity and inflammation. Type 1 or type 2 immune responses and their respective cytokines have been linked to white or brown AT, respectively. Most dramatic is the involvement of type-2 innate lymphoid cells (ILC2s) in stimulating eosinophil recruitment via interleukin (IL)-13 which in turn stimulates alternative macrophage activation via IL-4/IL-13. Recruited leukocytes are capable of influencing the cellular composition and function of adipose tissue and present a route to combat human obesity, however these processes are poorly understood in ruminants. Here we have characterised the resident leukocytes populations within bovine mesenteric AT (MAT) and subcutaneous AT (SAT), compared with the corresponding mesenteric lymph node (MLN). Concurring with related studies, we find bovine AT has its own resident leukocyte populations where eosinophils and neutrophils dominate. Importantly the proportion of eosinophils or neutrophils corresponded to the adipocyte size found in both depots. Further exploration of this area may have important implications on the food production industry or could be applied to improve the course of pathogenesis during disease.
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Bovinos/inmunología , Inmunidad Innata , Grasa Intraabdominal/citología , Leucocitos/inmunología , Grasa Subcutánea/citología , Adipocitos , Crianza de Animales Domésticos , Animales , Interleucina-13/inmunología , Interleucina-13/metabolismo , Interleucina-4/inmunología , Interleucina-4/metabolismo , Grasa Intraabdominal/inmunología , Recuento de Leucocitos , Leucocitos/metabolismo , Ganglios Linfáticos/citología , Ganglios Linfáticos/inmunología , Activación de Macrófagos , Masculino , Mesenterio/citología , Mesenterio/inmunología , Grasa Subcutánea/inmunologíaRESUMEN
OBJECTIVE: Inflammatory stimuli inducing epithelial-to-mesenchymal transition (EMT) can transdifferentiate mesenteric mesothelial cells into macrophages. METHODS: Sprague Dawley rat mesenteric mesothelial cells were used as a model. 1 ml Freund adjuvant was injected into the peritoneal cavity of rat and GM-CSF treatment was used to induce inflammation. IL-10 and IL-6 expression were studied by immunocytochemistry and Western blot analysis both in vivo and in vitro. RESULTS: Control mesothelial cell express anti-inflammatory IL-10, but no pro-inflammatory IL-6 expression could be detected in them. By the time of inflammation, IL-6 expression increased (reached the maximum level at the fifth day of inflammation), parallel to this the IL-10 entirely disappeared from these cells. In vitro GM-CSF treatment resulted in similar changes. As the mesothelial cells started to recover (at the eighth day of inflammation) IL-6 expression decreased and IL-10 level started to increase again. CONCLUSION: These data show that under inflammatory stimuli mesothelial cells-like macrophages-can produce pro-inflammatory cytokines.
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Células Epiteliales/fisiología , Interleucina-6/metabolismo , Macrófagos/metabolismo , Mesenterio/citología , Animales , Transdiferenciación Celular , Células Cultivadas , Transición Epitelial-Mesenquimal , Interleucina-10/metabolismo , Masculino , RatasRESUMEN
Regulatory B cells that produce IL-10 are now recognized as an important component of the immune system. We previously confirmed that IL-10 secreting CD21+ regulatory B cells (Breg cells) were present in ovine jejunal Peyer's patches (JPP) and this IL-10 production suppressed IL-12 and IFN-γ secretion. It is not known, however, whether ovine Breg cells are restricted to JPP or are present in other lymphoid tissues. Therefore, CD21+ B cells were purified from sheep JPP and from a variety of mucosal and systemic lymphoid tissues using magnetic cell sorting. Purified CD21+ B cells were stimulated with a TLR9-agonist, CpG oligodeoxynucleotide (CpG ODN), and the frequency of spontaneous and inducible (i) IL-10-secreting B cells was evaluated by ELISPOT. Spontaneous IL-10 secreting CD21+ B cells were present in mucosal (jejunal PP, parabronchial lymph nodes (LN), mesesnteric LN, and palatine tonsils) and systemic (spleen and blood) lymphoid tissues. Mucosal lymphoid tissues (parabronchial and mesenteric LNs and JPP) had the highest frequency of cells spontaneously secreting IL-10 while tonsils had the lowest. The frequency of B cells spontaneously secreting IL-10 was lowest in blood and spleen. There was large inter-animal variation in the frequency of CD21+ B cells spontaneously secreting IL-10 and no significant difference was detected following CpG ODN stimulation. When comparing within individual animals there was, however, a consistent increase in the frequency of CD21+ cells secreting IL-10 following CpG ODN stimulation versus stimulation with GpC control ODN. The presence of inducible (i)Breg cells in ovine mucosal tissues supports previous evidence from mice indicating that B cells have the capacity to modulate inflammatory responses. The presence of iBreg cells in ruminants may also provide a novel therapeutic target for both immunomodulatory drugs and vaccines designed to control antigen-specific mucosal inflammation.
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Linfocitos B Reguladores/inmunología , Tejido Linfoide/citología , Ovinos/inmunología , Animales , Linfocitos B Reguladores/efectos de los fármacos , Linfocitos B Reguladores/fisiología , Ensayo de Immunospot Ligado a Enzimas/veterinaria , Femenino , Citometría de Flujo/veterinaria , Mucosa Intestinal/citología , Mucosa Intestinal/inmunología , Ganglios Linfáticos/citología , Ganglios Linfáticos/inmunología , Tejido Linfoide/inmunología , Masculino , Mesenterio/citología , Mesenterio/inmunología , Oligodesoxirribonucleótidos/farmacología , Bazo/citología , Bazo/inmunologíaRESUMEN
The importance of immune cells present in the adipose tissue to metabolic homeostasis has been increasingly recognized. Nevertheless, in bovines few studies have so far addressed the immune cell populations resident in this tissue. Here we developed an eight-colour flow cytometry panel to address T cell populations present in bovine adipose tissue. Our results showed that γδ T cells, CD4+ and CD8+ CD3+ non-γδ T cells, as well as NK cells, are present in the mesenteric and subcutaneous adipose tissue of Holstein-Friesian cows. The frequency of both γδ T cells and CD8+ non-γδ T cells was found higher in mesenteric than in subcutaneous adipose tissue. The majority of T cells in adipose tissue presented a CD45RO+CD62L- phenotype, characteristic of effector memory cells, and the frequency of these cellular populations was higher than in the blood. The ratio of CD4+ T cells over CD8+ T cells was similar between subcutaneous and mesenteric adipose tissue but different from the one found in blood. Overall, our results highlight particular phenotypic characteristics of bovine adipose tissue T cell populations.
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Mesenterio/citología , Grasa Subcutánea/citología , Linfocitos T/citología , Animales , Bovinos , Células Cultivadas , Femenino , Citometría de Flujo , Inmunohistoquímica , Leucocitos Mononucleares/citologíaRESUMEN
CONTEXT: There is a correlation between prognosis of the colorectal carcinomas and the number of retrieved and metastatic lymph nodes (LNs) from mesentery/mesorectal region. At least 12 LNs must be sampled for accurate evaluation of patients. A number of factors related to surgeon, pathologist, patient and disease could affect the total LN number. For maximizing LN yield, pathologist can use ancillary methods, as fat clearance and special solutions. AIMS: This study investigates the effect of second evaluation after ethanol fixation on total and metastatic LN number and assesses factors that influence the dissected LN number. MATERIALS AND METHODS: 177 colorectal resections were refixed with ethanol for a night, after standard LN sampling. Mesentery/mesorectal tissue was reevaluated for missed LNs. Results were statistically analyzed, P values <0.05 were considered significant. RESULTS: Mean LN number increased from 26 to 30 (median: 20 to 25, P < 0.001) after ethanol fixation. Fourteen cases had additional metastatic LNs after reevaluation of the fat tissue and 5 of them upstaged. 22.5% (44/177) of the patients had <12 LNs before ethanol fixation and this decreased to 14.3% (26/177) after ethanol fixation. Resection type and length, tumor localization, size and histologic degree, pT and neoadjuvant therapy (P < 0.001) had an impact on the LN number (P = 0.034 for histologic degree, P = 0.02 for pT, P < 0.001 for others). CONCLUSIONS: Carrying out a second evaluation with ethanol fixation increased total and metastatic LN number and could lead upstage of pN. Ethanol fixation is cost-effective, easy accessible and applicable method; it may improve accuracy of LN assessment and staging, which are important for patients' outcome.
Asunto(s)
Neoplasias Colorrectales/diagnóstico , Ganglios Linfáticos/patología , Metástasis Linfática/diagnóstico , Mesenterio/citología , Adulto , Anciano , Anciano de 80 o más Años , Neoplasias Colorrectales/patología , Etanol , Femenino , Técnicas Histológicas , Humanos , Escisión del Ganglio Linfático , Metástasis Linfática/patología , Masculino , Mesenterio/patología , Persona de Mediana Edad , PronósticoRESUMEN
Leptin, the adipose tissue-derived product of the obese (ob) gene, is known to function as the hormone of energy expenditure. It has also been established that leptin regulates immune and inflammatory processes. All leptin-induced biological activities depend on binding to the membrane-spanning leptin receptor (Ob-R), belonging to the class I cytokine receptor family. The available data relating to the Ob-R on mature mast cells (MCs), and consequently leptin significance in the modulation of MC activity within the tissue, are limited. Immunohistochemistry was used to establish Ob-R expression by MCs in the mesenteric adipose tissue. Flow cytometry and confocal microscopy were used to evaluate both constitutive and leptin-induced expression of Ob-R on freshly isolated peritoneal MCs. MCs in the mesenteric adipose tissue and native peritoneal MCs express Ob-R constitutively. Additionally, leptin influences its receptor expression on these cells. Leptin at lower concentrations caused Ob-R expression increase both at the cell surface and in the cell interior. MC stimulation with higher concentrations of leptin results in a decline of Ob-R from the cell surface and significant enhancement of this receptor not only in the nuclear region but also in the endoplasmic reticulum. In conclusion, one can be assumed that leptin regulates MC activity within tissues. These findings might provide an additional link among the leptin, innate immune function, and inflammatory processes and diseases.
Asunto(s)
Tejido Adiposo/citología , Mastocitos/inmunología , Receptores de Leptina/metabolismo , Animales , Células Cultivadas , Metabolismo Energético , Femenino , Inmunidad Innata , Leptina/metabolismo , Mesenterio/citología , Peritoneo/citología , Transporte de Proteínas , Ratas , Ratas Wistar , Agregación de ReceptoresRESUMEN
In our previous work, we showed that during inflammation-induced epithelial-to-mesenchymal transition (EMT), mesenteric mesothelial cells express ED1 (pan-macrophage marker), indicating that they are transformed into macrophage-like cells. In this paper, we provide additional evidences about this transition by following the phagocytic activity and the TNFα production of mesenteric mesothelial cells during inflammation. Upon injection of India ink particles or fluorescent-labeled bioparticles (pHrodo) into the peritoneal cavity of rats pretreated with Freund's adjuvant, we found that mesothelial cells efficiently engulfed these particles. A similar increase of internalization could be observed by mesothelial cells in GM-CSF pretreated primary mesenteric culture. Since macrophages are the major producers of tumor necrosis factor, TNFα, we investigated expression level of TNFα during inflammation-induced EMT and found that TNFα was indeed expressed in these cells, reaching the highest level at the 5th day of inflammation. Since TNFα is one of the target genes of early growth response (EGR1) transcription factor, playing important role in monocyte-macrophage differentiation, expression of EGR1 in mesothelial cells was also investigated by Western blot and immunocytochemistry. While mesothelial cells did not express EGR1, a marked increase was observed in mesothelial cells by the time of inflammation. Parallel to this, nuclear translocation of EGR1 was shown by immunocytochemistry at the day 5 of inflammation. Caveolin-1 level was high and ERK1/2 became phosphorylated as the inflammation proceeded showing a slight decrease when the regeneration started. Our present data support the idea that under special stimuli, mesenteric mesothelial cells are able to transdifferentiate into macrophages, and this transition is regulated by the caveolin-1/ERK1/2/EGR1 signaling pathway.