Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Más filtros











Base de datos
Intervalo de año de publicación
1.
Braz J Med Biol Res ; 48(5): 382-91, 2015 May.
Artículo en Inglés | MEDLINE | ID: mdl-25760027

RESUMEN

Lung cancer often exhibits molecular changes, such as the overexpression of the ErbB1 gene that encodes epidermal growth factor receptor (EGFR). ErbB1 amplification and mutation are associated with tumor aggressiveness and low response to therapy. The aim of the present study was to design a schedule to synchronize the cell cycle of A549 cell line (a non-small cell lung cancer) and to analyze the possible association between the micronuclei (MNs) and the extrusion of ErbB1 gene extra-copies. After double blocking, by the process of fetal bovine serum deprivation and vincristine treatment, MNs formation was monitored with 5-bromo-2-deoxyuridine (BrdU) incorporation, which is an S-phase marker. Statistical analyses allowed us to infer that MNs may arise both in mitosis as well as in interphase. The MNs were able to replicate their DNA and this process seemed to be non-synchronous with the main cell nuclei. The presence of ErbB1 gene in the MNs was evaluated by fluorescent in situ hybridization (FISH). ErbB1 sequences were detected in the MNs, but a relation between the MNs formation and extrusion of amplified ErbB1 could not be established. The present study sought to elucidate the meaning of MNs formation and its association with the elimination of oncogenes or other amplified sequences from the tumor cells.


Asunto(s)
Antimetabolitos/metabolismo , Bromodesoxiuridina/metabolismo , Ciclo Celular/genética , Silenciador del Gen/fisiología , Genes erbB-1/genética , Micronúcleos con Defecto Cromosómico , Animales , Bovinos , Ciclo Celular/efectos de los fármacos , Línea Celular Tumoral , Replicación del ADN , Fase G1 , Amplificación de Genes/fisiología , Humanos , Hibridación Fluorescente in Situ , Micronúcleos con Defecto Cromosómico/inducido químicamente , Microscopía Confocal , Moduladores de la Mitosis/farmacología , Índice Mitótico/estadística & datos numéricos , Fase S , Vincristina/farmacología
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA