Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 28
Filtrar
1.
Neuromuscul Disord ; 31(8): 736-751, 2021 08.
Artículo en Inglés | MEDLINE | ID: mdl-34384671

RESUMEN

The DE50-MD canine model of Duchenne muscular dystrophy (DMD) has a dystrophin gene splice site mutation causing deletion of exon 50, an out-of-frame transcript and absence of dystrophin expression in striated muscles. We hypothesized that the musculoskeletal phenotype of DE50-MD dogs could be detected using Magnetic Resonance Imaging (MRI), that it would progress with age and that it would reflect those in other canine models and DMD patients. 15 DE50-MD and 10 age-matched littermate wild type (WT) male dogs underwent MRI every 3 months from 3 to 18 months of age. Normalized muscle volumes, global muscle T2 and ratio of post- to pre-gadolinium T1-weighted SI were evaluated in 7 pelvic limb and 4 lumbar muscles bilaterally. DE50-MD dogs, compared to WT, had smaller volumes in all muscles, except the cranial sartorius; global muscle T2 was significantly higher in DE50-MD dogs compared to WT. Muscle volumes plateaued and global muscle T2 decreased with age. Normalized muscle volumes and global muscle T2 revealed significant differences between groups longitudinally and should be useful to determine efficacy of therapeutics in this model with suitable power and low sample sizes. Musculoskeletal changes reflect those of DMD patients and other dog models.


Asunto(s)
Imagen por Resonancia Magnética , Músculo Esquelético/diagnóstico por imagen , Distrofia Muscular Animal/diagnóstico por imagen , Distrofia Muscular de Duchenne/diagnóstico por imagen , Animales , Modelos Animales de Enfermedad , Perros , Masculino , Músculo Esquelético/patología
2.
J Am Heart Assoc ; 8(16): e012443, 2019 08 20.
Artículo en Inglés | MEDLINE | ID: mdl-31411085

RESUMEN

Background Duchenne muscular dystrophy (DMD) is an X-linked disease that causes progressive muscle weakness. Affected boys typically die from respiratory or cardiac failure. Golden retriever muscular dystrophy (GRMD) is genetically homologous with DMD and causes analogous skeletal and cardiac muscle disease. Previous studies have detailed features of GRMD cardiomyopathy in mostly young dogs. Cardiac disease is not well characterized in adult GRMD dogs, and cardiac magnetic resonance (CMR) imaging studies have not been completed. Methods and Results We evaluated echocardiography and CMR in 24 adult GRMD dogs at different ages. Left ventricular systolic and diastolic functions, wall thickness, and myocardial strain were assessed with echocardiography. Features evaluated with CMR included left ventricular function, chamber size, myocardial mass, and late gadolinium enhancement. Our results largely paralleled those of DMD cardiomyopathy. Ejection fraction and fractional shortening correlated well with age, with systolic dysfunction occurring at ≈30 to 45 months. Circumferential strain was more sensitive than ejection fraction in early disease detection. Evidence of left ventricular chamber dilatation provided proof of dilated cardiomyopathy. Late gadolinium enhancement imaging showed DMD-like left ventricular lateral wall lesions and earlier involvement of the anterior septum. Multiple functional indexes were graded objectively and added, with and without late gadolinium enhancement, to give cardiac and cardiomyopathy scores of disease severity. Consistent with DMD, there was parallel skeletal muscle involvement, as tibiotarsal joint flexion torque declined in tandem with cardiac function. Conclusions This study established parallels of progressive cardiomyopathy between dystrophic dogs and boys, further validating GRMD as a model of DMD cardiac disease.


Asunto(s)
Cardiomiopatía Dilatada/veterinaria , Enfermedades de los Perros/diagnóstico por imagen , Distrofia Muscular Animal/diagnóstico por imagen , Factores de Edad , Animales , Técnicas de Imagen Cardíaca , Cardiomiopatía Dilatada/diagnóstico por imagen , Cardiomiopatía Dilatada/fisiopatología , Progresión de la Enfermedad , Enfermedades de los Perros/fisiopatología , Perros , Ecocardiografía , Femenino , Imagen por Resonancia Magnética , Imagen por Resonancia Cinemagnética , Masculino , Músculo Esquelético/fisiopatología , Distrofia Muscular Animal/fisiopatología , Distrofia Muscular de Duchenne
3.
Muscle Nerve ; 60(5): 621-628, 2019 11.
Artículo en Inglés | MEDLINE | ID: mdl-31397906

RESUMEN

INTRODUCTION: Golden retriever muscular dystrophy (GRMD) is a spontaneous X-linked canine model of Duchenne muscular dystrophy that resembles the human condition. Muscle percentage index (MPI) is proposed as an imaging biomarker of disease severity in GRMD. METHODS: To assess MPI, we used MRI data acquired from nine GRMD samples using a 4.7 T small-bore scanner. A machine learning approach was used with eight raw quantitative mapping of MRI data images (T1m, T2m, two Dixon maps, and four diffusion tensor imaging maps), three types of texture descriptors (local binary pattern, gray-level co-occurrence matrix, gray-level run-length matrix), and a gradient descriptor (histogram of oriented gradients). RESULTS: The confusion matrix, averaged over all samples, showed 93.5% of muscle pixels classified correctly. The classification, optimized in a leave-one-out cross-validation, provided an average accuracy of 80% with a discrepancy in overestimation for young (8%) and old (20%) dogs. DISCUSSION: MPI could be useful for quantifying GRMD severity, but careful interpretation is needed for severe cases.


Asunto(s)
Músculo Esquelético/diagnóstico por imagen , Distrofia Muscular Animal/diagnóstico por imagen , Animales , Modelos Animales de Enfermedad , Perros , Imagen por Resonancia Magnética , Músculo Esquelético/patología , Distrofia Muscular Animal/patología , Distrofia Muscular de Duchenne/diagnóstico por imagen , Distrofia Muscular de Duchenne/patología , Índice de Severidad de la Enfermedad
4.
IEEE Trans Biomed Eng ; 66(5): 1222-1230, 2019 05.
Artículo en Inglés | MEDLINE | ID: mdl-30235115

RESUMEN

OBJECTIVE: Histology is often used as a gold standard to evaluate noninvasive imaging modalities such as a magnetic resonance imaging (MRI). Spatial correspondence between histology and MRI is a critical step in quantitative evaluation of skeletal muscle in golden retriever muscular dystrophy (GRMD). Registration becomes technically challenging due to nonorthogonal histology section orientation, section distortion, and the different image contrast and resolution. METHODS: This study describes a three-step procedure to register histology images with multiparametric MRI, i.e., interactive slice localization controlled by a three-dimensional mouse, followed by an affine transformation refinement, and a B-spline deformable registration using a new similarity metric. This metric combines mutual information and gradient information. RESULTS: The methodology was verified using ex vivo high-resolution multiparametric MRI with a resolution of 117.19 µm (i.e., T1-weighted and T2-weighted MRI images) and trichrome stained histology images acquired from the pectineus muscles of ten dogs (nine GRMD and one healthy control). The proposed registration method yielded a root mean squares (RMS) error of 148.83 ± 34.96 µm averaged for ten muscle samples based on landmark points validated by five observers. The best RMS error averaged for ten muscles, was 128.48 ± 25.39 µm. CONCLUSION: The established correspondence between histology and in vivo MRI enables accurate extraction of MRI characteristics for histologically confirmed regions (e.g., muscle, fibrosis, and fat). SIGNIFICANCE: The proposed methodology allows creation of a database of spatially registered multiparametric MRI and histology. This database will facilitate accurate monitoring of disease progression and assess treatment effects noninvasively.


Asunto(s)
Enfermedades de los Perros/diagnóstico por imagen , Técnicas Histológicas , Interpretación de Imagen Asistida por Computador/métodos , Imagen por Resonancia Magnética , Distrofia Muscular Animal/diagnóstico por imagen , Animales , Perros , Técnicas Histológicas/métodos , Técnicas Histológicas/veterinaria , Imagen por Resonancia Magnética/métodos , Imagen por Resonancia Magnética/veterinaria
5.
Muscle Nerve ; 59(3): 380-386, 2019 03.
Artículo en Inglés | MEDLINE | ID: mdl-30461036

RESUMEN

INTRODUCTION: Golden retriever muscular dystrophy (GRMD), an X-linked recessive disorder, causes similar phenotypic features to Duchenne muscular dystrophy (DMD). There is currently a need for a quantitative and reproducible monitoring of disease progression for GRMD and DMD. METHODS: To assess severity in the GRMD, we analyzed texture features extracted from multi-parametric MRI (T1w, T2w, T1m, T2m, and Dixon images) using 5 feature extraction methods and classified using support vector machines. RESULTS: A single feature from qualitative images can provide 89% maximal accuracy. Furthermore, 2 features from T1w, T2m, or Dixon images provided highest accuracy. When considering a tradeoff between scan-time and computational complexity, T2m images provided good accuracy at a lower acquisition and processing time and effort. CONCLUSIONS: The combination of MRI texture features improved the classification accuracy for assessment of disease progression in GRMD with evaluation of the heterogenous nature of skeletal muscles as reflection of the histopathological changes. Muscle Nerve 59:380-386, 2019.


Asunto(s)
Enfermedades de los Perros/diagnóstico por imagen , Procesamiento de Imagen Asistido por Computador/métodos , Imagen por Resonancia Magnética/métodos , Distrofia Muscular Animal/diagnóstico por imagen , Animales , Biomarcadores , Perros , Músculo Esquelético/diagnóstico por imagen , Distrofia Muscular de Duchenne/patología , Máquina de Vectores de Soporte
6.
Annu Int Conf IEEE Eng Med Biol Soc ; 2018: 648-651, 2018 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-30440480

RESUMEN

Duchenne muscular dystrophy (DMD) is a fatal Xlinked muscle disorder caused by mutations in the dystrophin gene with a consequence of progressive degeneration of skeletal and cardiac muscle. Golden retriever muscular dystrophy (GRMD) is a spontaneous X-linked canine model of DMD with similar effects. Due to high soft-tissue contrast images, MRI is preferred as a non-invasive method to extract information corresponding to biological characteristics. We propose and evaluate non-invasive MRI-based imaging biomarkers to assess the severity of golden retriever muscular dystrophy (GRMD) using 3T and 4.7T MRI data of nine animals. These imaging biomarkers use first order statistics and texture (assessed by wavelets) in quantitative MRI (qMRI). In a leave-one-sampleout cross-validation framework, we use SVM to differentiate between young and old GRMD animals. The preliminary results show good differentiation between young and old animals for different qMRI sequences and based on a different selection of features.


Asunto(s)
Distrofia Muscular Animal/diagnóstico por imagen , Distrofia Muscular de Duchenne/diagnóstico por imagen , Animales , Biomarcadores , Modelos Animales de Enfermedad , Enfermedades de los Perros/diagnóstico por imagen , Perros , Imagen por Resonancia Magnética , Músculo Esquelético/diagnóstico por imagen
8.
Nat Commun ; 8(1): 1249, 2017 11 01.
Artículo en Inglés | MEDLINE | ID: mdl-29093487

RESUMEN

Muscular dystrophies (MDs) are often characterized by impairment of both skeletal and cardiac muscle. Regenerative strategies for both compartments therefore constitute a therapeutic avenue. Mesodermal iPSC-derived progenitors (MiPs) can regenerate both striated muscle types simultaneously in mice. Importantly, MiP myogenic propensity is influenced by somatic lineage retention. However, it is still unknown whether human MiPs have in vivo potential. Furthermore, methods to enhance the intrinsic myogenic properties of MiPs are likely needed, given the scope and need to correct large amounts of muscle in the MDs. Here, we document that human MiPs can successfully engraft into the skeletal muscle and hearts of dystrophic mice. Utilizing non-invasive live imaging and selectively induced apoptosis, we report evidence of striated muscle regeneration in vivo in mice by human MiPs. Finally, combining RNA-seq and miRNA-seq data, we define miRNA cocktails that promote the myogenic potential of human MiPs.


Asunto(s)
Corazón/crecimiento & desarrollo , Células Madre Pluripotentes Inducidas/citología , Mesodermo/citología , MicroARNs/genética , Desarrollo de Músculos/genética , Músculo Esquelético/crecimiento & desarrollo , Distrofia Muscular Animal/patología , Miocardio/citología , Animales , Diferenciación Celular , Ecocardiografía , Corazón/diagnóstico por imagen , Humanos , Ratones , Músculo Esquelético/citología , Músculo Esquelético/diagnóstico por imagen , Músculo Esquelético/patología , Distrofia Muscular Animal/diagnóstico por imagen , Miocardio/patología , Regeneración
9.
Am J Pathol ; 187(9): 2060-2070, 2017 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-28826559

RESUMEN

Genetic modifiers alter disease progression in both preclinical models and subjects with Duchenne muscular dystrophy (DMD). Using multiparametric magnetic resonance (MR) techniques, we compared the skeletal and cardiac muscles of two different dystrophic mouse models of DMD, which are on different genetic backgrounds, the C57BL/10ScSn-Dmdmdx (B10-mdx) and D2.B10-Dmdmdx (D2-mdx). The proton transverse relaxation constant (T2) using both MR imaging and spectroscopy revealed significant age-related differences in dystrophic skeletal and cardiac muscles as compared with their age-matched controls. D2-mdx muscles demonstrated an earlier and accelerated decrease in muscle T2 compared with age-matched B10-mdx muscles. Diffusion-weighted MR imaging indicated differences in the underlying muscle structure between the mouse strains. The fractional anisotropy, mean diffusion, and radial diffusion of water varied significantly between the two dystrophic strains. Muscle structural differences were confirmed by histological analyses of the gastrocnemius, revealing a decreased muscle fiber size and increased fibrosis in skeletal muscle fibers of D2-mdx mice compared with B10-mdx and control. Cardiac involvement was also detected in D2-mdx myocardium based on both decreased function and myocardial T2. These data indicate that MR parameters may be used as sensitive biomarkers to detect fibrotic tissue deposition and fiber atrophy in dystrophic strains.


Asunto(s)
Antecedentes Genéticos , Músculo Esquelético/diagnóstico por imagen , Distrofia Muscular Animal/diagnóstico por imagen , Animales , Progresión de la Enfermedad , Imagen por Resonancia Magnética , Masculino , Ratones , Ratones Endogámicos mdx , Distrofia Muscular Animal/genética
10.
Neuromuscul Disord ; 27(4): 315-325, 2017 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-28258941

RESUMEN

Because respiratory failure remains a major issue in Duchenne Muscular Dystrophy patients, respiratory muscles are a key target of systemic therapies. In the Golden Retriever Muscular Dystrophy (GRMD) dogs, the disease shows strong clinical and histological similarities with the human pathology, making it a valuable model for preclinical therapeutic trials. We report here the first nuclear magnetic resonance (NMR) imaging anatomical study of the diaphragm in GRMD dogs and healthy controls. Both T1- and T2-weighted images of the diaphragm of seven healthy and thirteen GRMD dogs, from 3 to 36 months of age, were acquired on a 3 tesla NMR scanner. Abnormalities of texture and shape were revealed and consisted of increases in signal intensity on T2-weighted images and in signal heterogeneity on both T1- and T2-weighted images of the dystrophic diaphragm. These abnormalities were associated with a significant thickening of the muscle and we identified a clear 8-mm-threshold distinguishing clinically preserved GRMD dogs from those more severely affected. In this study, we demonstrated the feasibility of NMR imaging of the diaphragm and depicted several anatomical and mesoscopic anomalies in the dystrophic diaphragm. NMR imaging of the diaphragm shows a promise as an outcome measure in preclinical trials using GRMD dogs.


Asunto(s)
Diafragma/diagnóstico por imagen , Distrofia Muscular Animal/diagnóstico por imagen , Animales , Perros , Distrofina/deficiencia , Imagen por Resonancia Magnética
11.
J Physiol ; 594(24): 7215-7227, 2016 12 15.
Artículo en Inglés | MEDLINE | ID: mdl-27570057

RESUMEN

KEY POINTS: Duchenne muscular dystrophy (DMD) is a severe, degenerative muscle disease that is commonly studied using the mdx mouse. The mdx diaphragm muscle closely mimics the pathophysiological changes in DMD muscles. mdx diaphragm force is commonly assessed ex vivo, precluding time course studies. Here we used ultrasonography to evaluate time-dependent changes in diaphragm function in vivo, by measuring diaphragm movement amplitude. In mdx mice, diaphragm amplitude decreased with age and values were much lower than for wild-type mice. Importantly, diaphragm amplitude strongly correlated with ex vivo specific force values. Micro-dystrophin administration increased mdx diaphragm amplitude by 26% after 4 weeks. Diaphragm amplitude correlated positively with ex vivo force values and negatively with diaphragm fibrosis, a major cause of DMD muscle weakness. These studies validate diaphragm ultrasonography as a reliable technique for assessing time-dependent changes in mdx diaphragm function in vivo. This technique will be valuable for testing potential therapies for DMD. ABSTRACT: Duchenne muscular dystrophy (DMD) is a severe, degenerative muscle disease caused by dystrophin mutations. The mdx mouse is a widely used animal model of DMD. The mdx diaphragm muscle most closely recapitulates key features of DMD muscles, including progressive fibrosis and considerable force loss. Diaphragm function in mdx mice is commonly evaluated by specific force measurements ex vivo. While useful, this method only measures force from a small muscle sample at one time point. Therefore, accurate assessment of diaphragm function in vivo would provide an important advance to study the time course of functional decline and treatment benefits. Here, we evaluated an ultrasonography technique for measuring time-dependent changes of diaphragm function in mdx mice. Diaphragm movement amplitude values for mdx mice were considerably lower than those for wild-type, decreased from 8 to 18 months of age, and correlated strongly with ex vivo specific force. We then investigated the time course of diaphragm amplitude changes following administration of an adeno-associated viral vector expressing Flag-micro-dystrophin (AAV-µDys) to young adult mdx mice. Diaphragm amplitude peaked 4 weeks after AAV-µDys administration, and was 26% greater than control mdx mice at this time. This value decreased slightly to 21% above mdx controls after 12 weeks of treatment. Importantly, diaphragm amplitude again correlated strongly with ex vivo specific force. Also, diaphragm amplitude and specific force negatively correlated with fibrosis levels in the muscle. Together, our results validate diaphragm ultrasonography as a reliable technique for assessing time-dependent changes in dystrophic diaphragm function in vivo, and for evaluating potential therapies for DMD.


Asunto(s)
Diafragma/diagnóstico por imagen , Diafragma/fisiopatología , Distrofia Muscular Animal/diagnóstico por imagen , Distrofia Muscular Animal/fisiopatología , Animales , Masculino , Ratones Endogámicos C57BL , Ratones Endogámicos mdx , Distrofia Muscular de Duchenne/diagnóstico por imagen , Distrofia Muscular de Duchenne/fisiopatología , Reproducibilidad de los Resultados , Ultrasonografía
12.
Sci Rep ; 5: 16625, 2015 Nov 13.
Artículo en Inglés | MEDLINE | ID: mdl-26564785

RESUMEN

Imaging of muscular structure with cellular or subcellular detail in whole-body animal models is of key importance for understanding muscular disease and assessing interventions. Classical histological methods for high-resolution imaging methods require excision, fixation and staining. Here we show that the three-dimensional muscular structure of unstained whole zebrafish can be imaged with sub-5 µm detail with X-ray phase-contrast tomography. Our method relies on a laboratory propagation-based phase-contrast system tailored for detection of low-contrast 4-6 µm subcellular myofibrils. The method is demonstrated on 20 days post fertilization zebrafish larvae and comparative histology confirms that we resolve individual myofibrils in the whole-body animal. X-ray imaging of healthy zebrafish show the expected structured muscle pattern while specimen with a dystrophin deficiency (sapje) displays an unstructured pattern, typical of Duchenne muscular dystrophy. The method opens up for whole-body imaging with sub-cellular detail also of other types of soft tissue and in different animal models.


Asunto(s)
Músculos/diagnóstico por imagen , Miofibrillas/diagnóstico por imagen , Intensificación de Imagen Radiográfica/métodos , Tomografía Computarizada por Rayos X/métodos , Animales , Modelos Animales de Enfermedad , Distrofina/deficiencia , Distrofina/genética , Imagenología Tridimensional/métodos , Larva/genética , Larva/metabolismo , Microscopía Confocal , Microscopía de Contraste de Fase , Distrofia Muscular Animal/diagnóstico por imagen , Distrofia Muscular Animal/genética , Distrofia Muscular de Duchenne/diagnóstico por imagen , Distrofia Muscular de Duchenne/genética , Intensificación de Imagen Radiográfica/instrumentación , Reproducibilidad de los Resultados , Tomografía Computarizada por Rayos X/instrumentación , Pez Cebra
13.
PLoS One ; 10(2): e0117835, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-25710816

RESUMEN

Quantitative nuclear magnetic resonance imaging (MRI) has been considered a promising non-invasive tool for monitoring therapeutic essays in small size mouse models of muscular dystrophies. Here, we combined MRI (anatomical images and transverse relaxation time constant-T2-measurements) to texture analyses in the study of four mouse strains covering a wide range of dystrophic phenotypes. Two still unexplored mouse models of muscular dystrophies were analyzed: The severely affected Largemyd mouse and the recently generated and worst double mutant mdx/Largemyd mouse, as compared to the mildly affected mdx and normal mice. The results were compared to histopathological findings. MRI showed increased intermuscular fat and higher muscle T2 in the three dystrophic mouse models when compared to the wild-type mice (T2: mdx/Largemyd: 37.6±2.8 ms; mdx: 35.2±4.5 ms; Largemyd: 36.6±4.0 ms; wild-type: 29.1±1.8 ms, p<0.05), in addition to higher muscle T2 in the mdx/Largemyd mice when compared to mdx (p<0.05). The areas with increased muscle T2 in the MRI correlated spatially with the identified histopathological alterations such as necrosis, inflammation, degeneration and regeneration foci. Nevertheless, muscle T2 values were not correlated with the severity of the phenotype in the 3 dystrophic mouse strains, since the severely affected Largemyd showed similar values than both the mild mdx and worst mdx/Largemyd lineages. On the other hand, all studied mouse strains could be unambiguously identified with texture analysis, which reflected the observed differences in the distribution of signals in muscle MRI. Thus, combined T2 intensity maps and texture analysis is a powerful approach for the characterization and differentiation of dystrophic muscles with diverse genotypes and phenotypes. These new findings provide important noninvasive tools in the evaluation of the efficacy of new therapies, and most importantly, can be directly applied in human translational research.


Asunto(s)
Imagen por Resonancia Magnética , Distrofia Muscular Animal/diagnóstico por imagen , Animales , Análisis por Conglomerados , Modelos Animales de Enfermedad , Femenino , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos mdx , Músculo Esquelético/diagnóstico por imagen , Músculo Esquelético/patología , Distrofia Muscular Animal/patología , Radiografía
14.
Vet Radiol Ultrasound ; 53(5): 492-500, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-22702494

RESUMEN

Golden retriever and Labrador retriever muscular dystrophy are inherited progressive degenerative myopathies that are used as models of Duchenne muscular dystrophy in man. Thoracic lesions were reported to be the most consistent radiographic finding in golden retriever dogs in a study where radiographs were performed at a single-time point. Muscular dystrophy worsens clinically over time and longitudinal studies in dogs are lacking. Thus our goal was to describe the thoracic abnormalities of golden retriever and Labrador retriever dogs, to determine the timing of first expression and their evolution with time. To this purpose, we retrospectively reviewed 390 monthly radiographic studies of 38 golden retrievers and six Labrador retrievers with muscular dystrophy. The same thoracic lesions were found in both golden and Labrador retrievers. They included, in decreasing frequency, flattened and/or scalloped diaphragmatic shape (43/44), pulmonary hyperinflation (34/44), hiatal hernia (34/44), cranial pectus excavatum (23/44), bronchopneumonia (22/44), and megaesophagus (14/44). The last three lesions were not reported in a previous radiographic study in golden retriever dogs. In all but two dogs the thoracic changes were detected between 4 and 10 months and were persistent or worsened over time. Clinically, muscular dystrophy should be included in the differential diagnosis of dogs with a combination of these thoracic radiographic findings.


Asunto(s)
Enfermedades de los Perros/diagnóstico por imagen , Distrofia Muscular Animal/diagnóstico por imagen , Radiografía Torácica/veterinaria , Envejecimiento , Animales , Bronconeumonía/diagnóstico por imagen , Bronconeumonía/etiología , Bronconeumonía/veterinaria , Diafragma/diagnóstico por imagen , Perros , Esófago/diagnóstico por imagen , Tórax en Embudo/diagnóstico por imagen , Tórax en Embudo/etiología , Tórax en Embudo/veterinaria , Pulmón/diagnóstico por imagen
15.
Ultrasonics ; 50(8): 741-3, 2010 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-20462623

RESUMEN

Duchenne muscular dystrophy (DMD) is caused by the absence of dystrophin, the protein that plays a key mechanical role in maintaining muscle membrane integrity. One of the major consequences of dystrophin deficiency is the degeneration of muscle fibres, with a progressive loss in muscle strength. The objective of this research was to find an ultrasonic parameter sensitive to DMD, which could give relevant information related to microstructure if compared to traditional investigations such as morphometrical analysis. This "in vitro" study focused on the Mdx mouse model and investigated the potential differences between wild-type and dystrophin-deficient mice diaphragms. Using a 50MHz ultrasonic sensor built in our group, we recorded an increase in ultrasonic wave attenuation in the dystrophin-deficient samples in comparison with normal muscles. A correlation between attenuation, mouse age and the percentage of non-muscular proportion in muscle was observed. As Mdx mouse is the best animal model for DMD and reproduces the degenerative pattern observed in human DMD muscles, this approach could be a powerful tool for in vitro DMD investigation and, more generally, for the characterisation of muscle properties.


Asunto(s)
Distrofia Muscular Animal/diagnóstico por imagen , Distrofia Muscular de Duchenne/diagnóstico por imagen , Animales , Modelos Animales de Enfermedad , Inmunohistoquímica , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos mdx , Músculo Esquelético/diagnóstico por imagen , Ultrasonografía
16.
J Ultrasound Med ; 28(6): 707-16, 2009 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-19470810

RESUMEN

OBJECTIVE: This study used high-frequency ultrasound (HFU) imaging to assess muscle damage noninvasively in a longitudinal study of 2 transgenic murine models of Duchenne muscular dystrophy (DMD): mdx, which has mutated cytoskeletal protein dystrophin; and udx, which has mutated dystrophin and lacks another cytoskeleton protein, utrophin. The mdx group was further subdivided into exercised and nonexercised subgroups to assess exercise-induced damage. METHODS: Muscle damage was assessed with HFU imaging (40 MHz) at biweekly intervals for 16 weeks. The assessment was based on the number of hyperechoic lesions, the lesion diameter, and muscle disorganization, giving a combined grade according to a 5-point scale. RESULTS: High-frequency ultrasound discriminated the severity of muscle damage between wild-type and transgenic models of DMD and between mdx and udx models. Qualitative comparisons of 3-dimensional HFU images with serial histologic sections of the skeletal muscle showed the ability of ultrasound to accurately depict changes seen in the muscle architecture in vivo. CONCLUSIONS: High-frequency ultrasound images soft tissue in mice at high contrast and spatial resolution, thereby showing that this microimaging modality has the capability to assess architectural changes in muscle fibers due to myotonic dystrophy-related diseases such as DMD.


Asunto(s)
Distrofia Muscular Animal/diagnóstico por imagen , Distrofia Muscular de Duchenne/diagnóstico por imagen , Animales , Modelos Animales de Enfermedad , Progresión de la Enfermedad , Distrofina/genética , Eliminación de Gen , Imagenología Tridimensional , Estudios Longitudinales , Ratones , Ratones Endogámicos mdx , Ratones Transgénicos , Distrofia Muscular Animal/genética , Distrofia Muscular Animal/fisiopatología , Distrofia Muscular de Duchenne/fisiopatología , Condicionamiento Físico Animal , Mutación Puntual , Ultrasonografía/métodos , Utrofina/genética
17.
Ultrasound Med Biol ; 35(3): 443-6, 2009 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-19081667

RESUMEN

In this study, we examined the correlation between muscle ultrasound and muscle structure. Echo intensity (EI) of 14 muscles of two golden retriever muscular dystrophy dogs was correlated to the percentage interstitial fibrous tissue and fat in muscle biopsy. A significant correlation between interstitial fibrous tissue and EI was found (r = 0.87; p < 0.001). The separate influence of interstitial fat on muscle EI could not be established as only little fat was present. We conclude that fibrous tissue causes increased muscle EI. The high correlation between interstitial fibrous tissue and EI makes ultrasound a reliable method to determine severity of structural muscle changes.


Asunto(s)
Músculo Esquelético/diagnóstico por imagen , Distrofia Muscular Animal/diagnóstico por imagen , Tejido Adiposo/diagnóstico por imagen , Tejido Adiposo/patología , Animales , Biopsia , Modelos Animales de Enfermedad , Perros , Fibrosis/diagnóstico por imagen , Fibrosis/patología , Masculino , Músculo Esquelético/patología , Distrofia Muscular Animal/patología , Reproducibilidad de los Resultados , Ultrasonografía
18.
J Anat ; 210(6): 723-30, 2007 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-17459142

RESUMEN

Skeletal anomalies are common in patients with muscular dystrophy, despite an absence of mutations to genes that specifically direct skeletogenesis. In order to understand these anomalies further, we examined two strains of muscular dystrophy (laminin- and merosin-deficient) relative to controls, to determine how the weakened muscle forces affected skull shape in a mouse model. Shape was characterized with geometric morphometric techniques, improving upon the limited analytical power of the standard linear measurements. Through these techniques, we document the specific types of cranial skeletal deformation produced by the two strains, each with individual shape abnormalities. The mice with merosin deficiency (with an earlier age of onset) developed skulls with more deformation, probably related to the earlier ontogenetic timing of disease onset. Future examinations of these mouse models may provide insight regarding the impact of muscular forces and the production and maintenance of craniofacial integration and modularity.


Asunto(s)
Huesos Faciales/diagnóstico por imagen , Interpretación de Imagen Asistida por Computador , Distrofia Muscular Animal/diagnóstico por imagen , Cráneo/diagnóstico por imagen , Animales , Distrofina/genética , Huesos Faciales/fisiopatología , Femenino , Laminina/metabolismo , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Mutantes , Distrofia Muscular Animal/metabolismo , Distrofia Muscular Animal/fisiopatología , Radiografía , Cráneo/fisiopatología
19.
Diabetes Obes Metab ; 9(3): 311-22, 2007 May.
Artículo en Inglés | MEDLINE | ID: mdl-17391157

RESUMEN

The influences of single-gene missense mutations expressing diabetes (db/db), obese (ob/ob) or dystrophia (dy/dy) dysregulated metabolic syndromes on hind limb bone maturation and cytodevelopment in C57BL/KsJ mice were evaluated by radiological, macro- and cytomorphometric analysis of the resulting variances in os coxae, femur and tibia osteodevelopment indices relative to control parameters between 8 and 16 weeks of age. Associated with obesity and hyperglycaemic/hyperinsulinaemic states, both db/db and ob/ob mutants demonstrated significant suppression of hind limb maturation (length) and cytodensity indices relative to control growth parameters. By contrast, skeletal growth suppression induced by dy/dy mutation expression was associated with lean body mass and normoglycaemic/hypoinsulinaemic systemic endometabolic indices. In both db/db and ob/ob mutation syndromes, osteovascular, -interstitial and -cytolipidaemia were prominent cytochemical aberrations of the osteopaenic states relative to the dyslipidaemia/fibrodysplasia characteristic of dy/dy osteomaturation. Between 8 and 16 weeks of age, both ob/ob and db/db groups demonstrated extensive cortical interstitial (laminal) osteolipidaemia and suppressed cytodensities compared to control indices. These data demonstrate that the abnormal hyperglycaemic/hyperinsulinaemic endometabolic states associated with the expression of db/db and ob/ob genomutations promote extensive lipidaemia-induced osteopaenia, compromising hind limb osteomaturation and cytodensity indices, as compared to the hyperfibritic osteopaenia characteristic of dy/dy mutation syndromes. Recognized therapeutic modulation of the hypercytolipidaemic component of diabetes-obesity syndromes may prove to be effective towards amelioration of the deleterious influences of these expressed hyperglycaemic, dysregulated lipometabolic conditions on osteomaturation and cytodevelopment.


Asunto(s)
Desarrollo Óseo/genética , Diabetes Mellitus Experimental/genética , Dislipidemias/genética , Distrofia Muscular Animal/genética , Mutación Missense/genética , Obesidad/genética , Animales , Diabetes Mellitus Experimental/diagnóstico por imagen , Diabetes Mellitus Experimental/patología , Femenino , Fémur/diagnóstico por imagen , Fémur/patología , Genotipo , Miembro Posterior , Cadera/diagnóstico por imagen , Cadera/patología , Histocitoquímica/métodos , Hiperlipidemias/genética , Ratones , Ratones Endogámicos C57BL , Distrofia Muscular Animal/diagnóstico por imagen , Obesidad/diagnóstico por imagen , Obesidad/patología , Radiografía , Tibia/diagnóstico por imagen , Tibia/patología
20.
Vet Radiol Ultrasound ; 47(6): 574-80, 2006.
Artículo en Inglés | MEDLINE | ID: mdl-17153067

RESUMEN

Golden Retriever muscular dystrophy is an inherited, degenerative myopathy due to the absence of dystrophin and is used as a model of Duchenne muscular dystrophy of young boys. This report describes the radiographic abnormalities of Golden Retriever muscular dystrophy in 26 dogs. The thoracic abnormalities included diaphragmatic asymmetry (18/26), diaphragmatic undulation (18/26), and gastro-esophageal hiatal hernia (6/26). Pelvic abnormalities included narrowing of the body of the ilia (14/19), ventral deviation and curvature of the tuber ischii (14/19), elongation of the obturator foramen with a decrease in opacity of the surrounding bone (12/19), and lateral flaring of the wings of the ilia (12/19). Abdominal abnormalities consisted of hepatomegaly (14/22) and poor serosal detail (12/22). The unique thoracic abnormalities were a consistent finding in affected Golden Retriever muscular dystrophy dogs. The diagnosis of muscular dystrophy should be included in the differential list if the combination of diaphragm undulation and asymmetry, and gastro-esophageal hiatal hernia are identified. These diaphragmatic abnormalities are related to hypertrophy and hyperplasia of the diaphragm. Additionally, the skeletal changes of pelvic tilt, elongation of the pelvis, widening of the obturator foramina and thinning of the ischiatic tables appear to be specific to Golden Retriever muscular dystrophy in dogs. These pelvic abnormalities are most likely secondary to bone remodeling associated with the progressive skeletal myopathy and subsequent contracture/fibrosis.


Asunto(s)
Enfermedades de los Perros/diagnóstico , Distrofia Muscular Animal/diagnóstico , Animales , Enfermedades de los Perros/diagnóstico por imagen , Perros , Femenino , Masculino , Distrofia Muscular Animal/diagnóstico por imagen , Linaje , Valor Predictivo de las Pruebas , Radiografía Torácica/veterinaria , Registros/veterinaria , Estudios Retrospectivos , Ultrasonografía
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA