RESUMEN
OBJECTIVE: To develop a quantitative PCR method for detecting hookworm infection and quantification. METHODS: A real-time PCR method was designed based on the intergenic region II of ribosomal DNA of the hookworm Necator americanus. The detection limit of this method was compared with the microscopy-based Kato-Katz method. The real-time PCR method was used to conduct an epidemiological survey of hookworm infection in southern Fujian Province of China. RESULTS: The real-time PCR method was specific for detecting Necator americanus infection, and was more sensitive than conventional PCR or microscopy-based method. A preliminary survey for hookworm infection in villages of Fujian Province confirmed the high prevalence of hookworm infections in the resident populations. In addition, the infection rate in women was significantly higher than that of in men. CONCLUSIONS: A real-time PCR method is designed, which has increased detection sensitivity for more accurate epidemiological studies of hookworm infections, especially when intensity of the infection needs to be considered.
Asunto(s)
ADN de Helmintos/genética , Microscopía , Necator americanus/aislamiento & purificación , Necatoriasis/diagnóstico , Técnicas de Amplificación de Ácido Nucleico , Reacción en Cadena en Tiempo Real de la Polimerasa , Animales , China/epidemiología , Femenino , Humanos , Masculino , Necator americanus/genética , Necatoriasis/epidemiología , Necatoriasis/genética , Sensibilidad y Especificidad , Vigilancia de Guardia , Análisis de Secuencia de ADN , Distribución por SexoRESUMEN
A six-month-old infant is reported with the unquestionable diagnosis diagnosis of Necator americanus uncinariasis. The mother was also infected with the same hookworm. The patient was born and has always dwelt in Mexico City, where helminthiasis is considered extremely rare. The epidemiologic studies determined that the dwelling conditions were inadequate for the transmittal of the agent through the soil. The authors emphasize the importance of the presumptive diagnosis in those cases suggesting the disease and to carry out studies to confirm the existence of other infectious means