RESUMEN
Trinucleotide repeat diseases such as myotonic dystrophy type 1 (DM1) and Huntington's disease (HD) are caused by expanded DNA repeats that can be used as templates to synthesize their own inhibitors. Because it would be particularly advantageous to reversibly assemble multivalent nucleic acid-targeting agents in situ, we sought to develop a target-guided screen that uses dynamic covalent chemistry to identify multitarget inhibitors. We report the synthesis of a library of amine- or aldehyde-containing fragments. The assembly of these fragments led to a diverse set of hit combinations that was confirmed by matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) in the presence of DM1 and HD repeat sequences. Of interest for both diseases, the resulting hit combinations inhibited transcription selectively and in a cooperative manner in vitro, with inhibitory concentration (IC50) values in the micromolar range. This dynamic covalent library and screening approach could be applied to identify compounds that reversibly assemble on other nucleic acid targets.
Asunto(s)
Aldehídos , Aminas , Ácidos Nucleicos , Aldehídos/síntesis química , Aldehídos/farmacología , Aminas/síntesis química , Aminas/farmacología , Evaluación Preclínica de Medicamentos , Humanos , Enfermedad de Huntington/genética , Distrofia Miotónica/genética , Ácidos Nucleicos/antagonistas & inhibidores , Ácidos Nucleicos/química , Secuencias Repetitivas de Ácidos Nucleicos , Transcripción Genética/efectos de los fármacosRESUMEN
Biofilms, the predominant growth mode of microorganisms, pose a significant risk to human health. The protective biofilm matrix, typically composed of exopolysaccharides, proteins, nucleic acids, and lipids, combined with biofilm-grown bacteria's heterogenous physiology, leads to enhanced fitness and tolerance to traditional methods for treatment. There is a need to identify biofilm inhibitors using diverse approaches and targeting different stages of biofilm formation. This review discusses discovery strategies that successfully identified a wide range of inhibitors and the processes used to characterize their inhibition mechanism and further improvement. Additionally, we examine the structure-activity relationship (SAR) for some of these inhibitors to optimize inhibitor activity.
Asunto(s)
Antibacterianos/farmacología , Biopelículas/efectos de los fármacos , Matriz Extracelular de Sustancias Poliméricas/efectos de los fármacos , Bacterias Gramnegativas/efectos de los fármacos , Bacterias Grampositivas/efectos de los fármacos , Bibliotecas de Moléculas Pequeñas/farmacología , Antibacterianos/biosíntesis , Antibacterianos/síntesis química , Antibacterianos/aislamiento & purificación , Proteínas Bacterianas/antagonistas & inhibidores , Proteínas Bacterianas/química , Proteínas Bacterianas/metabolismo , Biopelículas/crecimiento & desarrollo , GMP Cíclico/antagonistas & inhibidores , GMP Cíclico/química , GMP Cíclico/metabolismo , Diseño de Fármacos , Descubrimiento de Drogas , Farmacorresistencia Bacteriana/efectos de los fármacos , Matriz Extracelular de Sustancias Poliméricas/química , Matriz Extracelular de Sustancias Poliméricas/metabolismo , Bacterias Gramnegativas/crecimiento & desarrollo , Bacterias Gramnegativas/patogenicidad , Bacterias Grampositivas/crecimiento & desarrollo , Bacterias Grampositivas/patogenicidad , Lípidos/antagonistas & inhibidores , Lípidos/química , Pruebas de Sensibilidad Microbiana , Ácidos Nucleicos/antagonistas & inhibidores , Ácidos Nucleicos/química , Ácidos Nucleicos/metabolismo , Polisacáridos Bacterianos/antagonistas & inhibidores , Polisacáridos Bacterianos/química , Polisacáridos Bacterianos/metabolismo , Bibliotecas de Moléculas Pequeñas/química , Bibliotecas de Moléculas Pequeñas/aislamiento & purificación , Relación Estructura-ActividadRESUMEN
Nucleic acids have been widely recognized as potential targets in drug discovery and aptamer selection. Quantifying the interactions between small molecules and nucleic acids is critical to discover lead compounds and design novel aptamers. Scoring function is normally employed to quantify the interactions in structure-based virtual screening. However, the predictive power of nucleic acid-ligand scoring functions is still a challenge compared to other types of biomolecular recognition. With the rapid growth of experimentally determined nucleic acid-ligand complex structures, in this work, we develop a knowledge-based scoring function of nucleic acid-ligand interactions, namely SPA-LN. SPA-LN is optimized by maximizing both the affinity and specificity of native complex structures. The development strategy is different from those of previous nucleic acid-ligand scoring functions which focus on the affinity only in the optimization. The native conformation is stabilized while non-native conformations are destabilized by our optimization, making the funnel-like binding energy landscape more biased toward the native state. The performance of SPA-LN validates the development strategy and provides a relatively more accurate way to score the nucleic acid-ligand interactions.
Asunto(s)
Algoritmos , Descubrimiento de Drogas/métodos , Ácidos Nucleicos/química , Proyectos de Investigación , Sitios de Unión , Simulación por Computador , Humanos , Bases del Conocimiento , Ligandos , Simulación del Acoplamiento Molecular , Conformación de Ácido Nucleico , Ácidos Nucleicos/antagonistas & inhibidores , Sensibilidad y Especificidad , TermodinámicaRESUMEN
Our ever-increasing understanding of biological systems is providing a range of exciting novel biological targets, whose modulation may enable novel therapeutic options for many diseases. These targets include protein-protein and protein-nucleic acid interactions, which are, however, often refractory to classical small-molecule approaches. Other types of molecules, or modalities, are therefore required to address these targets, which has led several academic research groups and pharmaceutical companies to increasingly use the concept of so-called "new modalities". This Review defines for the first time the scope of this term, which includes novel peptidic scaffolds, oligonucleotides, hybrids, molecular conjugates, as well as new uses of classical small molecules. We provide the most representative examples of these modalities to target large binding surface areas such as those found in protein-protein interactions and for biological processes at the center of cell regulation.
Asunto(s)
Descubrimiento de Drogas , Ácidos Nucleicos/antagonistas & inhibidores , Oligonucleótidos/farmacología , Proteínas/antagonistas & inhibidores , Bibliotecas de Moléculas Pequeñas/farmacología , Humanos , Ácidos Nucleicos/química , Oligonucleótidos/química , Proteínas/química , Bibliotecas de Moléculas Pequeñas/química , Propiedades de SuperficieRESUMEN
Lupane derivatives containing an electronegative substituent in the position 2 of the skeleton are often cytotoxic, however, the most active compounds are not selective enough. To further study the influence of a substituent in the position 2 in lupane and 18α-oleanane derivatives on their biological properties, we prepared a set of 38 triterpenoid compounds, 19 of them new, most of them substituted in the position 2. From betulin, we obtained 2-bromo dihydrobetulonic acid and 2-bromo allobetulon and their substitutions yielded derivatives with various substituents in the position 2 such as amines, amides, thiols, and thioethers. Nitration of allobetulon and dihydrobetulonic acid gave 2-nitro and 2,2-dinitro derivatives. Fifteen derivatives had IC50 < 50 µM on a chemosensitive CCRF-CEM (acute lymphoblastic leukemia) cell line and were tested on another seven cancer cell lines including resistant and two non-cancer lines. 2-Amino allobetulin had IC50 4.6 µM and caused significant block of the tumor cells in S and slightly in G2/M transition and caused strong inhibition of DNA and RNA synthesis at 5 × IC50. 2-Amino allobetulin is the most active derivative of 18α-oleanane skeletal type prepared in our research group to date.
Asunto(s)
Antineoplásicos/química , Ácido Oleanólico/análogos & derivados , Triterpenos/química , Antineoplásicos/farmacología , Puntos de Control del Ciclo Celular/efectos de los fármacos , Línea Celular Tumoral , Citotoxinas/química , Citotoxinas/farmacología , Ensayos de Selección de Medicamentos Antitumorales , Humanos , Concentración 50 Inhibidora , Ácidos Nucleicos/antagonistas & inhibidores , Ácidos Nucleicos/biosíntesis , Ácido Oleanólico/química , Ácido Oleanólico/farmacología , Triterpenos/farmacologíaRESUMEN
PCR is an important and powerful tool in several fields, including clinical diagnostics, food analysis, and forensic analysis. In theory, PCR enables the detection of one single cell or DNA molecule. However, the presence of PCR inhibitors in the sample affects the amplification efficiency of PCR, thus lowering the detection limit, as well as the precision of sequence-specific nucleic acid quantification in real-time PCR. In order to overcome the problems caused by PCR inhibitors, all the steps leading up to DNA amplification must be optimized for the sample type in question. Sampling and sample treatment are key steps, but most of the methods currently in use were developed for conventional diagnostic methods and not for PCR. Therefore, there is a need for fast, simple, and robust sample preparation methods that take advantage of the accuracy of PCR. In addition, the thermostable DNA polymerases and buffer systems used in PCR are affected differently by inhibitors. During recent years, real-time PCR has developed considerably and is now widely used as a diagnostic tool. This technique has greatly improved the degree of automation and reduced the analysis time, but has also introduced a new set of PCR inhibitors, namely those affecting the fluorescence signal. The purpose of this chapter is to view the complexity of PCR inhibition from different angles, presenting both molecular explanations and practical ways of dealing with the problem. Although diagnostic PCR brings together scientists from different diagnostic fields, end-users have not fully exploited the potential of learning from each other. Here, we have collected knowledge from archeological analysis, clinical diagnostics, environmental analysis, food analysis, and forensic analysis. The concept of integrating sampling, sample treatment, and the chemistry of PCR, i.e., pre-PCR processing, will be addressed as a general approach to overcoming real-time PCR inhibition and producing samples optimal for PCR analysis.
Asunto(s)
Técnicas de Diagnóstico Molecular/métodos , Reacción en Cadena en Tiempo Real de la Polimerasa/métodos , Tampones (Química) , Humanos , Inhibidores de la Síntesis del Ácido Nucleico , Ácidos Nucleicos/antagonistas & inhibidores , Nucleótidos/antagonistas & inhibidores , Manejo de Especímenes/métodosRESUMEN
Brain tissue has become a challenging therapeutic target, in part because of failure of conventional treatments of brain tumors and a gradually increasing number of neurodegenerative diseases. Because antisense oligonucleotides are readily internalized by neuronal cells in culture, these compounds could possibly serve as novel therapeutic agents to meet such a challenge. In previous in vitro work using cell culture systems, we have demonstrated that intracellular delivery requires a vector such as cationic liposomes since free oligonucleotides remain largely trapped in the endocytic pathway following cellular uptake. Here we studied the cellular uptake properties of oligonucleotides by explants of rat brain (brain slices), and by in vivo brain tissue after administration of oligonucleotides by bolus injection. In contrast to in vitro uptake, we show that in brain slices oligonucleotides were taken up by neuronal and nonneuronal cells, irrespective of their assembly with cationic liposomes. In either case, a diffuse distribution of oligonucleotides was seen in the cytosol and/or nucleus. Uptake of oligonucleotides by brain slices as a result of membrane damage, potentially arising from the isolation procedure, could be excluded. Interestingly, internalization was inhibited following treatment of the tissue with antibody GN-2640, directed against a nucleic acid channel, present in rat kidney cells. Our data support the view that an analogous channel is present in brain tissue, allowing entry of free oligonucleotides but not plasmids. Indeed, for delivery of the latter and accomplishment of effective transfection, cationic lipids were needed for gene translocation into both brain slices and brain tissue in vivo. These data imply that for antisense therapy to become effective in brain, cationic lipid-mediated delivery will only be needed for specific cell targeting but not necessarily for delivery per se to accomplish nuclear deposition of oligonucleotides into brain cells and subsequent down-regulation of disease-related targets.
Asunto(s)
Encéfalo/metabolismo , Núcleo Celular/metabolismo , Ácidos Nucleicos/metabolismo , Oligonucleótidos/metabolismo , Transporte Activo de Núcleo Celular , Animales , Anticuerpos/farmacología , Cationes/química , Riñón/citología , Riñón/metabolismo , Lípidos/química , Ácidos Nucleicos/antagonistas & inhibidores , Oligonucleótidos/antagonistas & inhibidores , Oligonucleótidos/química , Plásmidos/química , Plásmidos/metabolismo , Ratas , Ratas Sprague-Dawley , Distribución TisularAsunto(s)
Antituberculosos , Diseño de Fármacos , Mycobacterium tuberculosis/efectos de los fármacos , Tuberculosis/tratamiento farmacológico , Animales , Antituberculosos/farmacología , Antituberculosos/uso terapéutico , Pared Celular/efectos de los fármacos , Pared Celular/metabolismo , Humanos , Nitroimidazoles/metabolismo , Ácidos Nucleicos/antagonistas & inhibidores , Ácidos Nucleicos/biosíntesis , Inhibidores de la Síntesis de la Proteína/farmacología , Quinolinas/farmacología , Quinolinas/uso terapéuticoRESUMEN
A number of proteins and nucleic acids have been explored as therapeutic targets. These targets are subjects of interest in different areas of biomedical and pharmaceutical research and in the development and evaluation of bioinformatics, molecular modeling, computer-aided drug design and analytical tools. A publicly accessible database that provides comprehensive information about these targets is therefore helpful to the relevant communities. The Therapeutic Target Database (TTD) is designed to provide information about the known therapeutic protein and nucleic acid targets described in the literature, the targeted disease conditions, the pathway information and the corresponding drugs/ligands directed at each of these targets. Cross-links to other databases are also introduced to facilitate the access of information about the sequence, 3D structure, function, nomenclature, drug/ligand binding properties, drug usage and effects, and related literature for each target. This database can be accessed at http://xin.cz3.nus.edu.sg/group/ttd/ttd.asp and it currently contains entries for 433 targets covering 125 disease conditions along with 809 drugs/ligands directed at each of these targets. Each entry can be retrieved through multiple methods including target name, disease name, drug/ligand name, drug/ligand function and drug therapeutic classification.
Asunto(s)
Bases de Datos de Ácidos Nucleicos , Bases de Datos de Proteínas , Quimioterapia , Humanos , Almacenamiento y Recuperación de la Información , Internet , Ligandos , Ácidos Nucleicos/antagonistas & inhibidores , Ácidos Nucleicos/química , Ácidos Nucleicos/fisiología , Proteínas/antagonistas & inhibidores , Proteínas/química , Proteínas/fisiología , Interfaz Usuario-ComputadorRESUMEN
OBJECTIVES: Na+,K+ pump inhibition is known to delay the development of apoptosis in vascular smooth muscle cells (VSMC). This study examines Na+,K+ pump involvement in the regulation of VSMC macromolecular synthesis and proliferation. METHODS: DNA, RNA and protein synthesis in VSMC from the rat aorta was studied by the incorporation of [3H]-labelled thymidine, uridine and leucine. Cell cycle progression was estimated by flow cytometry. Intracellular Na+ and K+ content and Na+,K+ pump activity were quantified as the steady-state distribution of 22Na and 86Rb and the rate of ouabain-sensitive 86Rb uptake in Na+-loaded cells, respectively. RESULTS: Ouabain inhibited the Na+,K+ pump with a Ki of 0.1 mmol/l. At concentrations less than 0.1 mmol/l, neither [Na+]i nor [K+]i was affected by ouabain; elevation of ouabain concentration sharply increased the [Na+]i/[K+]i ratio with a K0.5 of approximately 0.3 mmol/l. At concentrations higher than 0.1 mmol/l, ouabain time- and dose-dependently activated RNA and DNA syntheses in serum-deprived VSMC and inhibited cell cycle progression triggered by serum. In quiescent VSMC, ouabain did not affect protein synthesis, total cell number, but slightly increased the percentage of cells in the S-phase (4.25 versus 1.46%) and attenuated cell death assessed by staining with trypan blue and lactate dehydrogenase release. CONCLUSIONS: Elevation of the [Na+]i/[K+]i ratio caused by Na+,K+ pump inhibition markedly enhances nucleic acid synthesis in quiescent VSMC and blocks cell cycle progression in serum-supplied VSMC. The relative contribution of this phenomenon as well as the anti-apoptotic action of increased [Na+]i/[K+]i ratio to vascular remodelling under augmented content of endogenous Na+,K+ pump inhibitors, seen in volume-expanded hypertension, should be investigated by in-vivo studies.
Asunto(s)
Músculo Liso Vascular/citología , Músculo Liso Vascular/metabolismo , Ácidos Nucleicos/antagonistas & inhibidores , Ouabaína/farmacología , Potasio/metabolismo , ATPasa Intercambiadora de Sodio-Potasio/antagonistas & inhibidores , Sodio/metabolismo , Animales , Aorta/citología , Aorta/metabolismo , Vasos Sanguíneos/fisiopatología , Ciclo Celular/efectos de los fármacos , Muerte Celular/efectos de los fármacos , División Celular/efectos de los fármacos , Células Cultivadas , Membranas Intracelulares/metabolismo , Masculino , Ácidos Nucleicos/biosíntesis , Concentración Osmolar , Ratas , Ratas Endogámicas BNRESUMEN
Understanding the mode of action of and mechanism of resistance to drugs is central to optimising their use, and discovering new therapeutics with novel targets. We have limited understanding of how antimalarial drugs work and how resistance emerges. With few exceptions, antimalarial drugs in current use belong to a limited collection of chemical structures that act on a small number of partially characterised biochemical targets. Resistance has emerged to many of these compounds. The use of closely related compounds has promoted the spread of multidrug resistant parasites. This review intends to collate contemporary knowledge, and also to highlight conflicting views on unresolved issues.
Asunto(s)
Antimaláricos/farmacología , Artemisininas , Animales , Antimaláricos/uso terapéutico , Atovacuona , Resistencia a Medicamentos , Quimioterapia Combinada , Antagonistas del Ácido Fólico/farmacología , Antagonistas del Ácido Fólico/uso terapéutico , Humanos , Lactonas/farmacología , Lactonas/uso terapéutico , Mutación , Naftoquinonas/farmacología , Naftoquinonas/uso terapéutico , Ácidos Nucleicos/antagonistas & inhibidores , Plasmodium/efectos de los fármacos , Proguanil/farmacología , Proguanil/uso terapéutico , Quinolinas/farmacología , Quinolinas/uso terapéutico , Sesquiterpenos/farmacología , Sesquiterpenos/uso terapéuticoRESUMEN
Two lines of research were followed to obtain an adequate antisense effect. Natural oligonucleotides can degrade their mRNA targets catalytically, but are prone to enzymatic instability. Therefore, we looked for the minimal molecular modification, which would yield nuclease stable constructs, which can be taken up sufficiently to exert a selective antiproliferative effect. Small aliphatic diols conjugated at the 3'-end yielded such constructs, enabling selective tumor growth inhibition in an experimental in vivo situation. Further studies with complexing agents which increase their stability as well as uptake are ongoing. Another strategy aimed at obtaining strongly binding analogs, which would allow mRNA translation inhibition by steric interference. Hexitol nucleic acids (HNA) by virtue of their high and selective affinity for RNA, are therefore a prime candidate. When targeting the translation initiation regions, and using a lipofection protocol, nice activities were obtained at inhibiting Plasmodium, ICAM-1 and Ha-ras mRNA expression. Therefore, uptake should not be a limiting factor for the development of HNA as antisense therapeutics.
Asunto(s)
Elementos sin Sentido (Genética)/uso terapéutico , Ácidos Nucleicos/antagonistas & inhibidores , Oligonucleótidos Antisentido/uso terapéutico , ARN Mensajero/efectos de los fármacos , Tionucleótidos/genética , Animales , Regulación de la Expresión Génica , Humanos , Oligonucleótidos Antisentido/genética , Biosíntesis de ProteínasRESUMEN
In spite of forty years of effective chemotherapy for tuberculosis, the molecular mechanisms of antibacterial compounds in Mycobacterium tuberculosis have only recently been revealed. Broad spectrum antibacterials, including streptomycin, rifampicin, and fluoroquinolones have been demonstrated to act on the same targets in M. tuberculosis as they do in E. coli. Resistance to these agents results from single mutagenic events that lead to amino acid substitutions in their target proteins. The mechanisms of action of the unique antitubercular drugs, including isoniazid, ethambutol, and pyrazinamide have also recently been defined. Resistance to isoniazid can be caused either by mutations in the katG-encoded catalase-peroxidase, the enzyme responsible for drug activation, or by the molecular target, the inhA-encoded long chain enoyl-ACP reductase. Ethambutol appears to block specifically the biosynthesis of the arabinogalactan component of the mycobacterial cell envelope, and pyrazinamide has no known target. With the resurgence of tuberculosis and the appearance of strains which are multiply resistant to the above compounds, present tuberculosis chemotherapies are threatened. New approaches to the treatment of multi drug-resistant tuberculosis are needed.
Asunto(s)
Farmacorresistencia Microbiana/genética , Mycobacterium tuberculosis/efectos de los fármacos , Antituberculosos/farmacología , Pared Celular/efectos de los fármacos , Mycobacterium tuberculosis/genética , Ácidos Nucleicos/antagonistas & inhibidores , Ácidos Nucleicos/biosíntesis , Inhibidores de la Síntesis de la Proteína/farmacologíaRESUMEN
Low, mitogenic fluences of UVC (3.7-5.6 Jm-2) have previously been shown to cause increases of radioimmunoassayable transforming growth factor alpha (TGF alpha) in the medium and cells of cultures of melanocytes, melanoma lines, and HeLa cells (Ellem, K.A.O., Cullinan, M., Baumann, K.C., Dunstan, A.: Carcinogenesis 9:797-801, 1988). Here the cellular mechanism of this increase is explored by Northern blotting to detect any changes in TGF alpha mRNA levels, and the use of inhibitors of macromolecular synthesis to attempt to block the increase in TGF alpha protein. We were unable to detect any increase in TGF alpha mRNA levels attributable to UVC between 2 and 24 hours after irradiation. Inhibition of DNA synthesis (arabinosylcytosine, 10 microM), RNA synthesis (actinomycin D, 3 micrograms/ml; DRB 93 microM), or protein synthesis (cycloheximide, 10 micrograms/ml) failed to prevent the UVC induced increase in TGF alpha. We conclude that the UVC induction of TGF alpha is by a posttranslational mechanism. There was considerable discordance between the amount of TGF alpha protein and its mRNA in cultures of 15 different melanoma cell lines, which again emphasized that posttranscriptional mechanisms modulate the release of immunodetectable TGF alpha. We also found that the inhibitors themselves were capable of inducing an increase in TGF alpha in MM229 cultures. This suggests that the inhibitors and UV may effect the increase by a common mechanism, perhaps the activation of cell surface proteases as suggested for other stimuli (e.g., Pandiella, A., and Massagué, J.: Proc. Natl. Acad. Sci., USA 88:1726-1730, 1991) and that the response may be part of a global response to perturbation of DNA synthesis.
Asunto(s)
Melanoma/metabolismo , Biosíntesis de Proteínas , Factor de Crecimiento Transformador alfa/metabolismo , Rayos Ultravioleta , Muerte Celular , Humanos , Melanoma/patología , Ácidos Nucleicos/antagonistas & inhibidores , Proteínas/antagonistas & inhibidores , ARN Mensajero/metabolismo , Factores de Tiempo , Factor de Crecimiento Transformador alfa/genética , Células Tumorales Cultivadas/efectos de la radiaciónRESUMEN
Young and adult guinea pigs had been put in a chamber containing an 4-5 per cent addition of CO in the air. After 7 days of intoxication the authors performed a histopathological and histochemical examination of the retina. They discovered disturbances of the histological structure of the retina, a decrease of the content of nucleic acids and alkaline phosphatase and an increase of the acid phosphatase. The more pronounced intensification of the pathological changes was seen in the retina of younger individuals.
Asunto(s)
Fosfatasa Ácida/metabolismo , Contaminantes Atmosféricos/envenenamiento , Intoxicación por Monóxido de Carbono/metabolismo , Modelos Animales de Enfermedad , Retina/efectos de los fármacos , Factores de Edad , Fosfatasa Alcalina/antagonistas & inhibidores , Fosfatasa Alcalina/metabolismo , Animales , Cobayas , Ácidos Nucleicos/antagonistas & inhibidores , Ácidos Nucleicos/metabolismo , Retina/metabolismoRESUMEN
Sequence specific modification of nucleic acids with reactive oligonucleotide derivatives, complementary addressed modification, can provide an efficient approach for specific inactivation of certain cellular nucleic acids. In experiments with ascites tumor Krebs II cells and alkylating oligothymidylate derivatives it was found that alkylating oligonucleotide derivatives enter the living cell and modify complementary sequences in cellular nucleic acids with high efficiency. Complementary addressed modification of poly(A) sequences in cellular RNA with oligothymidylate derivatives was investigated in detail. The results of experiments on alkylation of cellular nucleic acids are consistent with complementary addressed modification of poly(A) sequences in cellular DNA. These results are supported by experiments on modification of chromatin DNA in which it was found that chromatin DNA interacts with oliogothymidylate derivatives more readily than the isolated double stranded DNA. It was found that alkylating oligonucleotide derivatives complementary to a sequence in immunoglobulin mRNA of MOPC 21 cells arrest the cellular immunoglobulin synthesis. Alkylating oligonucleotide derivatives complementary to RNAs of fowl plague virus inhibit virus multiplication in cell culture.