The adverse effect of overweight in assisted reproduction treatment outcomes.

OBJECTIVE: To assess Body Mass Index (BMI) effects on the results obtained from ICSI cycles. METHODS: We studied 266 ICSI cycles performed between January 2014 and December 2016. The patients were grouped according to their BMI in: Normal (18.5-24.9), Overweight (25.0-29.9) and Obese (>30). We compared the following variables between the groups: number of antral follicles, ovarian stimulation length, gonadotropin dose used, maximum estradiol level, follicles developed/antral follicles, retrieved oocytes/developed follicles and mature/retrieved oocytes, normal fertilization rate, embryo achieved/normal fertilized oocytes, clinical pregnancy and implantation rates. We used the Kruskal-Wallis and the Chi square tests. p<0.05 was considered significant. RESULTS: Normal, Overweight and Obese patients presented comparable values for number of antral follicles (11.6±5.4, 12.5±5.5, 12.2±5.7), ovarian stimulation length (7.5±1.4, 7.6±1.1, 7.8±1.3) and gonadotropin dose used (2043±489, 1940±536, 2109±605). Obese patients had lower values of estradiol (1560±610, 1511±635, 1190±466; p=0.018), developed follicles (81%, 76%, 70%; p<0.0001), and retrieved oocytes (91%, 90%, 84%; p=0.0017); and not significantly lower values of mature oocytes (82%, 82%, 77%; p=0.26). The groups had comparable fertilization rates (72%, 73%, 69%) and embryo achieved rates (67%, 63%, 72%). The normal group had higher, but not significantly higher pregnancy and implantation rates (43%, 40%, 38%, p=0.53; and 33%, 26%, 23%; p=0.11), and significantly higher ongoing pregnancy rates (37%, 33%, 33%, p=0.042). CONCLUSION: Increased BMI patients had impaired ovarian response and lower pregnancy rates in ICSI cycles.

Taxa de recuperação de oócitos de ovinos provenientes de abatedouros locais da região metropolitana de Teresina, Piauí, Brasil / Recovery rate of sheep oocyte from local abattoirs in metropolitan region of Teresina, Piauí, Brazil

In order to evaluate the recovery rate of local abattoirs oocyte the metropolitan region of Teresina-PI.Ovaries were used 72 36 pubescent sheep without defined breed (SRD) .The collected ovaries were transportedto the laboratory in thermos physiological saline (0.9% NaCl) heated at 35 ° C plus 30 ug / ml sulphategentamicin in a maximum of two (2) hours, the follicular aspiration needle attached syringe was subsequentlyperformed, the aspirated pellet was deposited on heated Petri dishes previously in hot plate to 37 ° C for theidentification and classification of oocitárias structures. 52 were recovered oocyte cumulus cells and the ewerecovery rate was 1.4. Therefore the low recovery rate can be the result of the female body condition, foodsupply, breeding age and age of the animal.

Taxa de recuperação de oócitos de ovinos provenientes de abatedouros locais da região metropolitana de Teresina, Piauí, Brasil / Recovery rate of sheep oocyte from local abattoirs in metropolitan region of Teresina, Piauí, Brazil

In order to evaluate the recovery rate of local abattoirs oocyte the metropolitan region of Teresina-PI.Ovaries were used 72 36 pubescent sheep without defined breed (SRD) .The collected ovaries were transportedto the laboratory in thermos physiological saline (0.9% NaCl) heated at 35 ° C plus 30 ug / ml sulphategentamicin in a maximum of two (2) hours, the follicular aspiration needle attached syringe was subsequentlyperformed, the aspirated pellet was deposited on heated Petri dishes previously in hot plate to 37 ° C for theidentification and classification of oocitárias structures. 52 were recovered oocyte cumulus cells and the ewerecovery rate was 1.4. Therefore the low recovery rate can be the result of the female body condition, foodsupply, breeding age and age of the animal.(AU)

Comparing stimulation requirements and final outcome between early follicular and mid luteal pituitary suppression in the long gonadotropin releasing hormone agonist protocol.

OBJECTIVE: To compare stimulation requirements and ICSI outcome when agonist treatment is started in the early follicular phase or in mid luteal phase of the cycle. METHODS: 181 infertile patients were randomly assigned to: group A (N=66) and group B (N=115). GnRH-a (Triptorelin) subcutaneous daily injections started on day 20-22 of the previous cycle till pituitary suppression is achieved where gonadotropins stimulation commenced. In group A, agonist treatment was started on the first or second days of the cycle, in group B it was started on day 20-22 of the cycle. The agonist treatment was continued till the day of (hCG) administration. RESULTS: The stimulation requirements were similar in the two groups. The days of t agonist treatment required to reach pituitary suppression were higher in group A: 12.5±6.4 than in group B, 11±4.5. Days of stimulation (10.4±1.7 and 10.3±1.6) and number of gonadotropin vials (40.1±8.7and 39.3±9.5) did not differ between both groups. The mean number of oocytes retrieved, mean number of embryos produced (11.7±7.4 and 13.3±9.3) (5.9±4.2and 6±5.2) were similar in both groups. The rates of fertilization and cleavage were similar in the two groups. Pregnancy rates were similar in both groups. The clinical pregnancy rates per cycle was 31.8% and 33%, while pregnancy rates per embryo transfer was 36.2 % and 36.5% in groups A and B respectively. CONCLUSION: Starting pituitary suppression with GnRH agonist in the early follicular phase or mid luteal phase were comparable regarding stimulation requirements and final outcomes.

[Comparative analysis of pregnancy rate/captured oocytes in an in vitro fertilization program]. / Análisis comparativo de la tasa de embarazo-ovocitos capturados en un programa de fertilización in vitro.

BACKGROUND: Since in vitro fertilization/embryo transfer is used as a common assisted reproductive technique there have been attempts to increase its success rate. One way is to obtain more good quality mature ovules to fertilize them, and two to three good quality embryos to transfer. OBJECTIVE: To determine if the number of retrieved oocytes is related with the pregnancy rate in IVF-ET. PATIENTS AND METHODS: Reproductive and descriptive study; 172 patients in the IVF program were included. Whole patients had ovary stimulation with FSHr and antagonist multidose protocol. Five study groups were considered depending on the oocyte number retrieved. Data were analized and correlated with fertilization and pregnancy rate. RESULTS: There were no statistical differences among age, body mass index, percentage of mature oocyte, fertilization rate, embryo cell stage or basal levels of LH and Estradiol. Group three showed the highest pregnancy rate (64.29%) nevertheless group five had major number of embryo transferred (2.97 +/- 0.54 vs 3.17 +/- 0.45, p = 0.21). According to FSH doses given, group one had statistical difference related to group three, with higher dose (54.1 vs 62.1). According to previous studies, related to the number of oocyte retrieved, the possibility of pregnancy is higher with more than 13 oocytes retrieved (OR: 0.9 IC 95%: 0.4 -1.7). CONCLUSIONS: Pregnancy rate is higher when ten to fifteen oocytes were retrieved.