RESUMEN
The present research deals with the development of a novel bioinspiredin situfabrication of reduced graphene oxide (rGO)-silver nanoparticle (AgNPs) nanocomposite (rGO@AgNCs) using microbes namelyPseudomonas aeruginosa(PA) andStaphylococcus aureus(SA). The fabricated rGO@AgNCs were characterized using Ultraviolet-visible (UV-Vis) spectroscopy, Fourier-transform infrared spectroscopy (FTIR), particle size analysis, polydispersity index (PDI), zeta potential analysis, energy dispersive x-ray analysis (EDAX), Raman spectroscopy, powder x-ray diffraction (PXRD), high-resolution transmission electron microscopy (HR-TEM) analysis, etc. Furthermore, the rGO@AgNCs-PA and rGO@AgNCs-SA interaction with serum protein, pH stability study, andin vitrodissolution of AgNPs were also performed. The research findings of the proposed study demonstrated the simultaneous reduction of graphene oxide (GO) and AgNPs and the formation of rGO@AgNCs in the presence of microbes. Thein vitrodissolution studies of rGO@AgNCs composites showed better AgNPs dissolution with controlled release and offered remarkable matrix integrity throughout the dissolution period. The size and stability of rGO@AgNCs-PA and rGO@AgNCs-SA had no significant changes at physiological pH 7.4. A minimal decrease in the zeta potential of rGO@AgNCs was observed, which may be due to the weak interaction of nanocomposites and albumin. The antibacterial application of the synthesized nanocomposite was evaluated against a pathogenic mastitis-forming bacterium. The obtained results suggested an admirable antibacterial activity of synthesized nanocomposites against the tested microbes. This knowledge will assist the scientific fraternity in designing novel antibacterial agents with enhanced antibacterial activity against various veterinary pathogens in near future.
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Antibacterianos/síntesis química , Grafito/síntesis química , Nanocompuestos/química , Óxidos/síntesis química , Compuestos de Plata/síntesis química , Tamaño de la Partícula , Pseudomonas aeruginosa , Staphylococcus aureusRESUMEN
Chemodynamic therapy (CDT), deemed as a cutting-edge antineoplastic therapeutic tactics, efficaciously suppresses tumors via catalytically yielding hydroxyl radicals (â¢OH) in tumor regions. Nevertheless, its biomedical applications are often restricted by the limited hydrogen peroxide (H2O2) level and upregulated antioxidant defense. Herein, a versatile nanoreactor is elaborately designed via integrating Cu2-xS and MnO2 for T1-weighted magnetic resonance (MR) imaging-guided CDT, synergistically enhanced through hypothermal ablation and oxidation resistance reduction, thereby displaying splendid antitumor efficiency as well as suppression on pulmonary metastasis. The as-synthesized Cu2-xS@MnO2 nanoreactors afford acid-dependent Cu-based and glutathione (GSH)-activated Mn-based catalytic properties for bimodal CDT. Owing to excellent absorbance at the second near-infrared (NIR-II) window, the Cu2-xS furnishes hypo-photo-thermal therapy (PTT) against tumor growth and ameliorates the catalytic performance for thermal-enhanced CDT. Additionally, MnO2 significantly downregulates GSH and glutathione peroxidase 4, which synergistically boosts CDT via promoting oxidative stress, simultaneously generating Mn2+ for MR contrast improvement and activatable tumor imaging. Therefore, this study proffers a new attempt centered on the collaborative strategy integrating NIR-II hypothermal PTT and synergistically enhanced CDT for tumor eradication.
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Antineoplásicos/farmacología , Cobre/farmacología , Compuestos de Manganeso/farmacología , Óxidos/farmacología , Fármacos Fotosensibilizantes/farmacología , Terapia Fototérmica , Animales , Antineoplásicos/síntesis química , Antineoplásicos/química , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Cobre/química , Ensayos de Selección de Medicamentos Antitumorales , Femenino , Humanos , Imagen por Resonancia Magnética , Neoplasias Mamarias Experimentales/diagnóstico por imagen , Neoplasias Mamarias Experimentales/tratamiento farmacológico , Neoplasias Mamarias Experimentales/metabolismo , Compuestos de Manganeso/síntesis química , Compuestos de Manganeso/química , Ratones , Ratones Endogámicos BALB C , Oxidación-Reducción , Óxidos/síntesis química , Óxidos/química , Fármacos Fotosensibilizantes/síntesis química , Fármacos Fotosensibilizantes/química , Células Tumorales CultivadasRESUMEN
Leishmaniasis is a neglected disease that affects 12 million people living mainly in developing countries. Herein, 24 new N-oxide-containing compounds were synthesized followed by in vitro and in vivo evaluation of their antileishmanial activity. Compound 4f, a furoxan derivative, was particularly remarkable in this regard, with EC50 value of 3.6 µM against L. infantum amastigote forms and CC50 value superior to 500 µM against murine peritoneal macrophages. In vitro studies suggested that 4f may act by a dual effect, by releasing nitric oxide after biotransformation and by inhibiting cysteine protease CPB (IC50: 4.5 µM). In vivo studies using an acute model of infection showed that compound 4f at 7.7 mg/Kg reduced ~90% of parasite burden in the liver and spleen of L. infantum-infected BALB/c mice. Altogether, these outcomes highlight furoxan 4f as a promising compound for further evaluation as an antileishmanial agent.
Asunto(s)
Antiprotozoarios/farmacología , Diseño de Fármacos , Leishmania infantum/efectos de los fármacos , Óxidos/farmacología , Animales , Antiprotozoarios/síntesis química , Antiprotozoarios/química , Biomarcadores/metabolismo , Espectroscopía de Resonancia Magnética con Carbono-13 , Ligandos , Macrófagos Peritoneales/efectos de los fármacos , Macrófagos Peritoneales/parasitología , Masculino , Ratones , Simulación del Acoplamiento Molecular , Óxido Nítrico/análisis , Nitritos/análisis , Oxadiazoles/síntesis química , Oxadiazoles/química , Óxidos/síntesis química , Óxidos/química , Carga de Parásitos , Pichia/metabolismo , Espectroscopía de Protones por Resonancia Magnética , Proteínas Protozoarias/metabolismoRESUMEN
Growing evidence across organisms points to altered energy metabolism as an adverse outcome of metal oxide nanomaterial toxicity, with a mechanism of toxicity potentially related to the redox chemistry of processes involved in energy production. Despite this evidence, the significance of this mechanism has gone unrecognized in nanotoxicology due to the field's focus on oxidative stress as a universalâbut nonspecificânanotoxicity mechanism. To further explore metabolic impacts, we determined lithium cobalt oxide's (LCO's) effects on these pathways in the model organism Daphnia magna through global gene-expression analysis using RNA-Seq and untargeted metabolomics by direct-injection mass spectrometry. Our results show that a sublethal 1 mg/L 48 h exposure of D. magna to LCO nanosheets causes significant impacts on metabolic pathways versus untreated controls, while exposure to ions released over 48 h does not. Specifically, transcriptomic analysis using DAVID indicated significant enrichment (Benjamini-adjusted p ≤0.0.5) in LCO-exposed animals for changes in pathways involved in the cellular response to starvation (25 genes), mitochondrial function (70 genes), ATP-binding (70 genes), oxidative phosphorylation (53 genes), NADH dehydrogenase activity (12 genes), and protein biosynthesis (40 genes). Metabolomic analysis using MetaboAnalyst indicated significant enrichment (γ-adjusted p <0.1) for changes in amino acid metabolism (19 metabolites) and starch, sucrose, and galactose metabolism (7 metabolites). Overlap of significantly impacted pathways by RNA-Seq and metabolomics suggests amino acid breakdown and increased sugar import for energy production. Results indicate that LCO-exposed Daphnia respond to energy starvation by altering metabolic pathways, both at the gene expression and metabolite levels. These results support altered energy production as a sensitive nanotoxicity adverse outcome for LCO exposure and suggest negative impacts on energy metabolism as an important avenue for future studies of nanotoxicity, including for other biological systems and for metal oxide nanomaterials more broadly.
Asunto(s)
Cobalto/farmacología , Daphnia/efectos de los fármacos , Nanoestructuras/química , Óxidos/farmacología , Animales , Cobalto/química , Daphnia/metabolismo , Metabolismo Energético , Óxidos/síntesis química , Óxidos/químicaRESUMEN
Microbial contamination is one of the major dreadful problems that raises hospitalization, morbidity and mortality rates globally, which subsequently obstructs socio-economic progress. The continuous misuse and overutilization of antibiotics participate mainly in the emergence of microbial resistance. To circumvent such a multidrug-resistance phenomenon, well-defined nanocomposite structures have recently been employed. In the current study, a facile, novel and cost-effective approach was applied to synthesize Ag@Ag2O core-shell nanocomposites (NCs) via chemical method. Several techniques were used to determine the structural, morphological, and optical characteristics of the as-prepared NCs. XRD, Raman, FTIR, XPS and SAED analysis revealed a crystalline hybrid structure of Ag core and Ag2O shell. Besides, SEM and HRTEM micrographs depicted spherical nanoparticles with size range of 19-60 nm. Additionally, zeta potential and fluorescence spectra illustrated aggregated nature of Ag@Ag2O NCs by - 5.34 mV with fluorescence emission peak at 498 nm. Ag@Ag2O NCs exhibited higher antimicrobial, antibiofilm, and algicidal activity in dose-dependent behavior. Interestingly, a remarkable mycocidal potency by 50 µg of Ag@Ag2O NCs against Candida albican; implying promising activity against COVID-19 white fungal post-infections. Through assessing cytotoxicity, Ag@Ag2O NCs exhibited higher safety against Vero cells than bulk silver nitrate by more than 100-fold.
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Antiinfecciosos/química , Antiinfecciosos/farmacología , Biopelículas/efectos de los fármacos , Nanocompuestos/química , Óxidos/química , Compuestos de Plata/química , Animales , Antiinfecciosos/síntesis química , Candida albicans/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Chlorella vulgaris/efectos de los fármacos , Chlorocebus aethiops , Desinfectantes/síntesis química , Desinfectantes/química , Desinfectantes/farmacología , Bacterias Gramnegativas/efectos de los fármacos , Bacterias Grampositivas/efectos de los fármacos , Nanopartículas del Metal/química , Óxidos/síntesis química , Pseudomonas aeruginosa/efectos de los fármacos , Compuestos de Plata/síntesis química , Nitrato de Plata/farmacología , Staphylococcus aureus/efectos de los fármacos , Células VeroRESUMEN
Rationale: Sensitive and accurate imaging of cancer is essential for early diagnosis and appropriate treatment. For generally employed magnetic resonance imaging (MRI) in clinic, comprehending how to enhance the contrast effect of T1 imaging is crucial for improving the sensitivity of cancer diagnosis. However, there is no study ever to reveal the clear mechanism of how to enhance the effect of T1 imaging and accurate relationships of influencing factors. Herein, this study aims to figure out key factors that affect the sensitivity of T1 contrast-enhanced MRI (CE-MRI), thereby to realize sensitive detection of tumors with low dose of CAs. Methods: Manganese oxide (MnO) nanoparticles (NPs) with various sizes and shapes were prepared by thermal decomposition. Factors impacting T1 CE-MRI were investigated from geometric volume, surface area, crystal face to r2/r1 ratio. T1 CE-MR imaging of liver, hepatic and subcutaneous tumors were conducted with MnO NPs of different shapes. Results: The surface area and occupancy rate of manganese ions have positive impacts on the sensitivity of T1 CE-MRI, while volume and r2/r1 ratio have negative effects. MnO octahedrons have a high r1 value of 20.07 mM-1s-1 and exhibit an excellent enhanced effect in liver T1 imaging. ZDS coating facilitates tumor accumulation and cellular uptake, hepatic and subcutaneous tumors could be detected with MnO octahedrons at an ultralow dose of 0.4 mg [Mn]/kg, about 1/10 of clinical dose. Conclusions: This work is the first quantitative study of key factors affecting the sensitivity of T1 CE-MRI of MnO nanoparticles, which can serve as a guidance for rational design of high-performance positive MRI contrast agents. Moreover, these MnO octahedrons can detect hepatic and subcutaneous tumors with an ultralow dose, hold great potential for sensitive and accurate diagnosis of cancer with lower cost, less dosages and side effects in clinic.
Asunto(s)
Medios de Contraste/química , Neoplasias Hepáticas/diagnóstico por imagen , Imagen por Resonancia Magnética/métodos , Compuestos de Manganeso/química , Nanopartículas del Metal/química , Óxidos/química , Animales , Línea Celular Tumoral , Humanos , Neoplasias Hepáticas/diagnóstico , Masculino , Compuestos de Manganeso/síntesis química , Nanopartículas del Metal/ultraestructura , Ratones , Ratones Endogámicos BALB C , Microscopía Electrónica de Transmisión , Óxidos/síntesis química , Tamaño de la PartículaRESUMEN
Cobalt oxide nanoparticles (CoO NPs) were synthesized by the calcination method from the Co (II) complex which has the formula [Co(PVA)(P-ABA)(H2O)3], PVA = polyvinyl alcohol, and P-ABA = para-aminobenzoic acid. The calcination temperature was 550°C, and the products were characterized by element analysis, thermal analyses (TGA and DTA), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), UV-Vis spectra, and scanning electron microscopy (SEM) techniques. The kinetic and thermodynamic parameters (∆H ∗ , ∆G ∗ , and ∆S ∗ ) for the cobalt (II) complex are calculated. The charges been carried by the atoms cause dipole moment 10.53 and 3.84 debye and total energy 11.04 × 102 and 24.80 × 102 k Cal mol-1 for the Co (II) complex and cobalt oxide, respectively. X-ray diffraction confirmed that the resulting oxide was pure single-crystalline CoO nanoparticles. Scanning electron microscopy indicating that the crystallite size of cobalt oxide nanocrystals was in the range of 36-54 nm. Finally, the antimicrobial activity of cobalt oxide nanoparticles was evaluated using four bacterial strains and one fungal strain. Two strains of Gram-positive cocci (Staphylococcus aureus and Enterococcus faecalis), two strains of Gram-negative bacilli (Escherichia coli and Pseudomonas aeruginosa), and one strain of yeast such as fungi (Candida albicans) were used in this study.
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Ácido 4-Aminobenzoico/química , Antibacterianos/síntesis química , Nanopartículas del Metal/química , Óxidos/síntesis química , Alcohol Polivinílico/química , Antibacterianos/farmacología , Candida albicans/efectos de los fármacos , Candida albicans/crecimiento & desarrollo , Cobalto/farmacología , Enterococcus faecalis/efectos de los fármacos , Enterococcus faecalis/crecimiento & desarrollo , Escherichia coli/efectos de los fármacos , Escherichia coli/crecimiento & desarrollo , Cinética , Nanopartículas del Metal/ultraestructura , Pruebas de Sensibilidad Microbiana , Óxidos/farmacología , Pseudomonas aeruginosa/efectos de los fármacos , Pseudomonas aeruginosa/crecimiento & desarrollo , Staphylococcus aureus/efectos de los fármacos , Staphylococcus aureus/crecimiento & desarrollo , TermodinámicaRESUMEN
Besides the pandemic caused by the coronavirus outbreak, many other pathogenic microbes also pose a devastating threat to human health, for instance, pathogenic bacteria. Due to the lack of broad-spectrum antibiotics, it is urgent to develop nonantibiotic strategies to fight bacteria. Herein, inspired by the localized "capture and killing" action of bacteriophages, a virus-like peroxidase-mimic (V-POD-M) is synthesized for efficient bacterial capture (mesoporous spiky structures) and synergistic catalytic sterilization (metal-organic-framework-derived catalytic core). Experimental and theoretical calculations show that the active compound, MoO3 , can serve as a peroxo-complex-intermediate to reduce the free energy for catalyzing H2 O2 , which mainly benefits the generation of â¢OH radicals. The unique virus-like spikes endow the V-POD-M with fast bacterial capture and killing abilities (nearly 100% at 16 µg mL-1 ). Furthermore, the in vivo experiments show that V-POD-M possesses similar disinfection treatment and wound skin recovery efficiencies to vancomycin. It is suggested that this inexpensive, durable, and highly reactive oxygen species (ROS) catalytic active V-POD-M provides a promising broad-spectrum therapy for nonantibiotic disinfection.
Asunto(s)
Antibacterianos/síntesis química , Materiales Biomiméticos/síntesis química , Óxidos/síntesis química , Peroxidasa/química , Antibacterianos/farmacología , Materiales Biocompatibles/química , Materiales Biomiméticos/farmacología , Catálisis , Humanos , Peróxido de Hidrógeno/metabolismo , Estructuras Metalorgánicas/química , Estructuras Metalorgánicas/farmacología , Simulación de Dinámica Molecular , Molibdeno/farmacología , Óxidos/farmacología , Peroxidasa/metabolismo , Esterilización , Vancomicina/farmacologíaRESUMEN
KEY MESSAGE: Ca2+ NPs enhanced tolerance of Triticale callus under salt stress by improving biochemical activity and confocal laser scanning analysis, conferring salt tolerance on callus cells. CaO NPs (Ca2+) are significant components that act as transducers in many adaptive and developmental processes in plants. In this study, effect of Ca2+ NPs on the response and regulation of the protective system in Triticale callus under short and long-salt treatments was investigated. The activation of Ca2+ NPs was induced by salt stress in callus of Triticale cultivars. MDA, H2O2, POD, and protein activities were determined in callus tissues. Concerning MDA, H2O2, protein activities, it was found that the Ca2+ NPs treatment was significant, and it demonstrated a high correlation with the tolerance levels of cultivars. Tatlicak cultivar was detected for better MDA activities in the short time with 1.5 ppm Ca2+ NPs concentration of 50 g and 100 g NaCl. Similarly, the same cultivar responded with better H2O2 activity at 1.5 ppm Ca2+ NPs 100 g NaCl in the short time. POD activities exhibited a decreasing trend in response to the increasing concentrations of Ca2+ NPs. The best result was observed at 1.5 ppm Ca2+ NPs 100 g NaCl in the short term. Based on the protein content, treatment of short-term cultured callus cells with 1.5 ppm Ca2+ NPs inhibited stress response and it significantly promoted Ca2+ NPs signals as compared to control callus. Confocal laser scanning analysis proved that the application of Ca2+ NPs could alleviate the adverse effects of salt stress by the inhibition of stress severity in callus cells. This study demonstrated, under in vitro conditions, that the application of Ca2+ NPs can significantly suppress the adverse effects of salt stress on Triticale callus; it was also verified that the concentration of Ca2+ NPs could be important parameter to be considered in adjusting the micronutrient content in the media for this plant.
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Compuestos de Calcio/farmacología , Nanopartículas/química , Óxidos/farmacología , Estrés Salino/fisiología , Triticale/efectos de los fármacos , Triticale/fisiología , Compuestos de Calcio/síntesis química , Compuestos de Calcio/química , Peróxido de Hidrógeno/metabolismo , Malondialdehído/metabolismo , Microscopía Confocal , Microscopía Electrónica de Rastreo , Óxidos/síntesis química , Óxidos/química , Proteínas de Plantas/metabolismo , Estrés Salino/efectos de los fármacos , Espectroscopía Infrarroja por Transformada de Fourier , Triticale/citología , Difracción de Rayos XRESUMEN
In an effort to explore a new class of antidiabetic inhibitors, a new series of isoxazolidine and C-alkyl imine oxide derivatives scaffolds were designed, synthesized and fully characterized. The newly synthesized analogues were evaluated for their human pancreatic α-amylase (HPA) and human lysosomal acid-α-glucosidase (HLAG) inhibitory activities and have shown a higher potency than acarbose. The compounds 7b (23.1 ± 1.1 µM) and 7a (36.3 ± 1.6 µM) were identified as the potent HPA and HLAG inhibitors with inhibitory effect up to 9 and 21-fold higher than acarbose, respectively. Antihyperglycemic activity results were supported by molecular docking approach of the most potent compounds 7b and 7a showing stronger interactions with the active site of HPA and HLAG as well as by in silico absorption, distribution, metabolism, excretion and toxicity (ADMET) profile suggesting their satisfactory oral druglikeness without toxic effect. Therefore, it can be concluded that both 7b and 7a can be used as effective lead molecules for the development of HPA and HLAG inhibitors for the management of T2DM.
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Diseño de Fármacos , Inhibidores de Glicósido Hidrolasas/farmacología , Hipoglucemiantes/farmacología , Iminas/farmacología , Isoxazoles/farmacología , Óxidos/farmacología , Relación Dosis-Respuesta a Droga , Inhibidores de Glicósido Hidrolasas/síntesis química , Inhibidores de Glicósido Hidrolasas/química , Humanos , Hipoglucemiantes/síntesis química , Hipoglucemiantes/química , Iminas/síntesis química , Iminas/química , Isoxazoles/síntesis química , Isoxazoles/química , Lisosomas/enzimología , Simulación del Acoplamiento Molecular , Estructura Molecular , Óxidos/síntesis química , Óxidos/química , Páncreas/enzimología , Relación Estructura-Actividad , alfa-Amilasas/antagonistas & inhibidores , alfa-Amilasas/metabolismo , alfa-Glucosidasas/metabolismoRESUMEN
Double perovskites have been extensively studied in materials chemistry due to their excellent properties and novel features attributed to the coexistence of ferro/ferri/antiferro-magnetic ground state and semiconductor band gap within the same material. Double perovskites with Sr2NiMO6 (M = Te, W) structure type have been synthesized using simple, non-toxic and costless aqueous citrate sol-gel route. The reaction yielded phase-pure nanocrystalline powders of two compounds: Sr2NiWO6 (SNWO) and Sr2NiTeO6 (SNTO). According to the Rietveld refinement of powder X-ray diffraction data at room temperature, Sr2NiWO6 is tetragonal (I4/m) and Sr2NiTeO6 is monoclinic (C12/m1), with average crystallite sizes of 49 and 77 nm, respectively. Structural studies have been additionally performed by Raman spectroscopy revealing optical phonons typical for vibrations of Te6+/W6+O6 octahedra. Both SNTO and SNWO possess high values of dielectric constants (341 and 308, respectively) with low dielectric loss (0.06 for SNWO) at a frequency of 1 kHz. These values decrease exponentially with the increase of frequency to 1000 kHz, with the dielectric constant being around 260 for both compounds and dielectric loss being 0.01 for SNWO and 0.04 for SNTO. The Nyquist plot for both samples confirms the non-Debye type of relaxation behavior and the dominance of shorter-range movement of charge carriers. Magnetic studies of both compounds revealed antiferromagnetic behavior, with Néel temperature (TN) being 57 K for SNWO and 35 K for SNTO.
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Compuestos de Calcio/química , Imanes/química , Nanopartículas/química , Óxidos/química , Titanio/química , Compuestos de Calcio/síntesis química , Cristalización , Espectroscopía Dieléctrica , Modelos Moleculares , Óxidos/síntesis química , Espectrometría por Rayos X , Espectrometría Raman , Temperatura , Difracción de Rayos XRESUMEN
With the growing interest of the medical industry in biocompatible nanoparticles (NPs), the current synthetic methods should be adapted to appropriate demands (toxicity, scalability, etc.). Most applications require colloidal systems to be stable not only in water but also in vivo, which represents a major challenge. In this study, biocompatible Ta2O5 NPs were synthesized by a solvothermal method avoiding toxic reagents, and surfactant-free stable hydrosols were obtained and used for computed tomography (CT) imaging. The small hydrodynamic size (2 nm) and colloidal stability of primary NPs were studied by dynamic light scattering (DLS). The particles were characterized by X-ray diffraction, transmission electron microscopy, energy-dispersive X-ray spectroscopy, and Brunauer-Emmett-Teller analysis to confirm their structure and purity. To develop a stable hydrosol preparation protocol, the influence of pH and ultrasonication duration on the stability of Ta2O5 sols was analyzed by DLS and microelectrophoresis. To enhance the understanding of NP behavior in vivo, sol stability in conditions close to physiological (NaCl solutions) was studied in a pH range of 3-9. Hydrosols prepared by the proposed protocol were stable for at least 6 months and exhibited negligible cytotoxicity. Ta2O5 NPs also showed high CT contrast both in theoretical calculations and in vivo (rat gastrointestinal tract).
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Coloides/química , Medios de Contraste/química , Tracto Gastrointestinal/diagnóstico por imagen , Nanopartículas del Metal/química , Óxidos/química , Tantalio/química , Animales , Línea Celular , Coloides/síntesis química , Coloides/toxicidad , Medios de Contraste/síntesis química , Medios de Contraste/toxicidad , Humanos , Nanopartículas del Metal/toxicidad , Óxidos/síntesis química , Óxidos/toxicidad , Ratas , Tantalio/toxicidad , Tomografía Computarizada por Rayos X/métodosRESUMEN
Natural or synthetic biomaterials are increasingly being used to support bone tissue repair or substitution. The combination of natural calcium phosphates with biocompatible alloys is an important route towards the development of new biomaterials with bioperformance and mechanical responses to mimic those of human bones. This article evaluated the structural, physical, mechanical and biological properties of a new mechanical improved nanocomposite elaborated by association of fish biphasic calcium phosphate (BCP) and niobium pentoxide (Nb2O5). The nanocomposite (Nb-BCP) and the pure BCP, used as a positive control, were obtained by powder metallurgy. The density, porosity and microhardness were measured. The structural analysis was determined by X-ray diffraction (XRD) and the biological properties were studied in histological sections of critical size calvaria defects in rats, 7, 15, 30, 45 and 60 days after implantation of disks of both materials. Morphological description was made after scanning electron microscopy (SEM) and optical microscopy analysis. After sintering, the Nb-BCP nanocomposite presented four crystalline phases: 34.36% calcium niobate (CaNb2O6), 21.68% phosphorus niobium oxide (PNb9O25), 42.55% ß-tricalcium phosphate (Ca3(PO4)2) and 1.31% of niobium pentoxide (Nb2O5) and exhibited increases of 17% in density, 66% in Vickers microhardness and 180% in compressive strength compared to pure BCP. In vivo study, showed biocompatibility, bioactivity and osteoconductivity similar to pure BCP. SEM showed the formation of globular accretions over the implanted nanocomposites, representing one of the stages of bone mineralization. In conclusion, the BCP and Nb2O5 formed a nanocomposite exhibiting characteristics that are desirable for a biomaterial, such as bioperformance, higher ß-TCP percentage and improved physical and mechanical properties compared to pure BCP. These characteristics demonstrate the promise of this material for supporting bone regeneration.
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Sustitutos de Huesos/química , Fosfatos de Calcio/química , Niobio/química , Oseointegración , Óxidos/química , Fracturas Craneales/terapia , Animales , Regeneración Ósea/efectos de los fármacos , Sustitutos de Huesos/farmacología , Sustitutos de Huesos/uso terapéutico , Prótesis Anclada al Hueso , Interfase Hueso-Implante/patología , Fosfatos de Calcio/síntesis química , Fosfatos de Calcio/uso terapéutico , Modelos Animales de Enfermedad , Hidroxiapatitas/síntesis química , Hidroxiapatitas/química , Hidroxiapatitas/uso terapéutico , Masculino , Ensayo de Materiales , Microscopía Electrónica de Rastreo , Nanocompuestos/química , Nanocompuestos/uso terapéutico , Niobio/uso terapéutico , Oseointegración/efectos de los fármacos , Óxidos/síntesis química , Óxidos/uso terapéutico , Ratas , Ratas Wistar , Fracturas Craneales/patología , Difracción de Rayos XRESUMEN
Iron plays an important role in various physiological processes. However, the detailed biological functions of iron have not been sufficiently explored because of a lack of effective methods to monitoring iron, especially the labile ferrous ion (Fe2+). In the current study, a novel turn-on phosphorescent probe for Fe2+ quantification and visualization has been proposed based on the hybrid nanocomposite of manganese dioxide and gemini iridium complex (MnO2-GM-Ir). The surfactant-like GM-Ir with positive charges was beneficial to combine with the negatively charged manganese dioxide (MnO2) nanosheets, and thus endowing the MnO2-GM-Ir nanocomposite excellent dispersion ability in the water as well as efficiently avoiding the interference to the detection caused by the agglomeration of nanocomposite. Phosphorescence of GM-Ir was effectively quenched by MnO2 nanosheets through fluorescence resonance energy transfer (FRET) and the inner filter effect (IFE), while the phosphorescence could be significantly recovered in the presence of Fe2+via a selective Fe2+-mediated reduction of MnO2 nanosheets, indicating a highly-specific selectivity towards Fe2+ with a low detection limit (80 nM). The drug test assay and in vitro imaging studies further proved that the MnO2-GM-Ir nanocomposite could be employed as a promising probe for the quantitative detection of exogenous Fe2+ in drug and in vitro imaging of living cells.
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Compuestos Ferrosos/análisis , Colorantes Fluorescentes/química , Imagen Óptica , Animales , Calcitriol/análogos & derivados , Calcitriol/química , Línea Celular Tumoral , Transferencia Resonante de Energía de Fluorescencia , Iones/análisis , Iridio/química , Compuestos de Manganeso/síntesis química , Compuestos de Manganeso/química , Ratones , Nanocompuestos/química , Óxidos/síntesis química , Óxidos/química , Tamaño de la Partícula , Espectrometría de Fluorescencia , Propiedades de SuperficieRESUMEN
In the present work, we report on the synthesis of crump-like nickel manganous oxide nanoparticles decorated partially reduced graphene oxide (NiMnO@pr-GO) nanocomposite through high-intensity ultrasonic bath sonication (ultrasonic frequency = 37 kHz and power = 150 W). The NiMnO@pr-GO nanocomposite modified glassy carbon electrode (GCE) was then employed for the electrochemical reduction of detrimental metronidazole (MNZ). The crystalline phase and formation of the NiMnO@pr-GO nanocomposites were confirmed by X-ray diffraction and other spectroscopic techniques. The cyclic voltammetry results demonstrate that this NiMnO@pr-GO nanocomposite modified GCE has a lower reduction potential and higher catalytic activity towards MNZ than do NiMnO and GO modified GCEs. Under optimized conditions, the fabricated NiMnO@pr-GO electrode can detect metronidazole over a wide linear range with a lower limit of detection of 90 nM. The sensitivity of the sensor was 1.22 µA µM-1cm-2 and was found to have excellent selectivity and durability for the detection of MNZ.
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Grafito/química , Compuestos de Manganeso/química , Compuestos de Manganeso/síntesis química , Metronidazol/química , Nanotecnología , Níquel/química , Óxidos/química , Óxidos/síntesis química , Ondas Ultrasónicas , Técnicas de Química Sintética , Electroquímica , Oxidación-ReducciónRESUMEN
In the current study, γ-AlOOH, γ-MnOOH, and α-Mn2O3 nanorods (NRs) were easily synthesized and applied as advanced antibacterial materials. γ-AlOOH NRs with 20 nm width, [100] crystal plane, and 200 nm length were fabricated through a surfactant-directed solvothermal method. γ-MnOOH NRs with 20 nm width, [101] crystal direction and 500 nm length were fabricated through a hydrothermal method. The prepared γ-MnOOH NRs were calcinated (for 5 h) at 700 °C to produce α-Mn2O3 NRs with 20 nm average width and increased surface area. The NRs' structures were confirmed through FT-IR, XRD, XPS, FESEM, and FETEM. The antibacterial activity of the NRs was studied against different Gram-negative and Gram-positive bacterial strains and yeast. The three NRs exhibited antibacterial activity against all of the used strains. Biological studies indicated that the NRs' antimicrobial activity increased in the order of γ-MnOOH < γ-AlOOH < α-Mn2O3 NRs. The α-Mn2O3 NRs exhibited the lowest MIC value (39 µg mL-1) against B. subtilis, B. pertussis, and P. aeruginosa. The prepared NRs exhibited a higher antimicrobial potential toward Gram-positive bacteria than Gram-negative bacteria. The higher antimicrobial activity of the α-Mn2O3 NRs is highlighted based on their larger surface area and smaller diameter. Consequently, uniform NR architectures, single crystallinity, small nanoscale diameters, and more highly exposed [110] Mn-polar surfaces outwards are promising structures for α-Mn2O3 antibacterial agents. These NRs adhered firmly to the bacterial cells causing cell wrapping and morphology disruption, and microbial death. The designed NRs provide a great platform for microbial growth inhibition.
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Hidróxido de Aluminio/farmacología , Óxido de Aluminio/farmacología , Antibacterianos/farmacología , Hidróxidos/farmacología , Compuestos de Manganeso/farmacología , Nanotubos/química , Óxidos/farmacología , Hidróxido de Aluminio/síntesis química , Hidróxido de Aluminio/química , Óxido de Aluminio/síntesis química , Óxido de Aluminio/química , Antibacterianos/síntesis química , Antibacterianos/química , Bacillus subtilis/efectos de los fármacos , Bordetella pertussis/efectos de los fármacos , Diseño de Fármacos , Hidróxidos/síntesis química , Hidróxidos/química , Compuestos de Manganeso/síntesis química , Compuestos de Manganeso/química , Nanopartículas del Metal/química , Pruebas de Sensibilidad Microbiana , Óxidos/síntesis química , Óxidos/química , Tamaño de la Partícula , Pseudomonas aeruginosa/efectos de los fármacos , Plata/química , Propiedades de SuperficieRESUMEN
The exceptional nature of WO3-x dots has inspired widespread interest, but it is still a significant challenge to synthesize high-quality WO3-x dots without using unstable reactants, expensive equipment, and complex synthetic processes. Herein, the synthesis of ligand-free WO3-x dots is reported that are highly dispersible and rich in oxygen vacancies by a simple but straightforward exfoliation of bulk WS2 and a mild follow-up chemical conversion. Surprisingly, the WO3-x dots emerged as co-reactants for the electrochemiluminescence (ECL) of Ru(bpy)32+ with a comparable ECL efficiency to the well-known Ru(bpy)32+ /tripropylamine (TPrA) system. Moreover, compared to TPrA, whose toxicity remains a critical issue of concern, the WO3-x dots were ca. 300-fold less toxic. The potency of WO3-x dots was further explored in the detection of circulating tumor cells (CTCs) with the most competitive limit of detection so far.
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Técnicas Biosensibles , Técnicas Electroquímicas , Mediciones Luminiscentes , Células Neoplásicas Circulantes/patología , Compuestos Organometálicos/química , Óxidos/química , Propilaminas/química , Tungsteno/química , Humanos , Óxidos/síntesis químicaRESUMEN
We synthesized all major saturated and unsaturated 2-alkyl-4(1H)-quinolone N-oxides of Pseudomonas and Burkholderia, quantified their native production levels and characterized their antibiotic activities against competing Staphylococcus aureus. We demonstrate that quinolone core methylation and position of unsaturation in the alkyl-chain dictate antibiotic potency which supports the proposed mechanism of action.
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Antibacterianos/farmacología , Burkholderia/efectos de los fármacos , Óxidos/farmacología , Pseudomonas/efectos de los fármacos , Quinolonas/farmacología , Antibacterianos/síntesis química , Antibacterianos/química , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Óxidos/síntesis química , Óxidos/química , Quinolonas/síntesis química , Quinolonas/química , EstereoisomerismoRESUMEN
Hypoxia within solid tumors severely limits the efficacy of photodynamic therapy (PDT). Biocompatible calcium peroxide nanoparticles (CaO2 NPs) have superior oxygen generating capacity for hypoxia relief but the relatively slow release of O2 from CaO2 NPs hampers the PDT efficacy enhancement. Herein, manganese dioxide (MnO2) is applied as a nanozyme to facilitate O2 release from CaO2 NPs. It is disclosed that the accelerated O2 release ensures a rapid and efficient amplification of the O2 level for an increased cytotoxic singlet oxygen production with chlorin e6 and leads to a down-regulated hypoxia-responsive protein expression, which eventually translates to a super-efficient PDT as evidenced by the complete eradication of mice bearing subcutaneous 4T1 tumors. Meanwhile, MnO2 imparts an MR T1 imaging modality for tumor detection and treatment planning. These findings signify the essential role of accelerated and efficient hypoxia relief in PDT efficacy enhancement and provide an effective paradigm to overcome hypoxia-associated resistance for an enhanced therapeutic efficacy.
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Antineoplásicos/farmacología , Neoplasias de la Mama/tratamiento farmacológico , Hipoxia de la Célula/efectos de los fármacos , Compuestos de Manganeso/farmacología , Nanopartículas/química , Óxidos/farmacología , Oxígeno/metabolismo , Peróxidos/metabolismo , Fotoquimioterapia , Animales , Antineoplásicos/síntesis química , Antineoplásicos/química , Materiales Biocompatibles/síntesis química , Materiales Biocompatibles/química , Materiales Biocompatibles/farmacología , Neoplasias de la Mama/metabolismo , Neoplasias de la Mama/patología , Catálisis , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Femenino , Colorantes Fluorescentes/química , Neoplasias Mamarias Experimentales/tratamiento farmacológico , Neoplasias Mamarias Experimentales/metabolismo , Neoplasias Mamarias Experimentales/patología , Compuestos de Manganeso/síntesis química , Compuestos de Manganeso/química , Ratones , Óxidos/síntesis química , Óxidos/química , Oxígeno/análisis , Peróxidos/químicaRESUMEN
Local surface plasmon resonance (LSPR)-enhanced catalysis has brought a substantial amount of opportunities across various disciplines such as photocatalysis, photodetection, and photothermal therapeutics. Plasmon-induced photothermal and hot carriers effects have also been utilized to activate the enzyme-like reactions. Compared with natural enzymes, the relatively low catalytic performance of nanozymes severely hampered the potential applications in the field of biomedicine. For these issues mentioned above, herein, we demonstrate a highly efficient sulfite oxidase (SuOx) mimetic performance of plasmonic monolayer MoOx (ML-MoOx) upon LSPR excitation. We also established that the considerable photothermal effect and the injection of hot carriers induced by LSPR are responsible for promoting the SuOx activity of ML-MoOx. The high transient local temperature on the surface of ML-MoOx generated by the photothermal effect facilitates to impact the reaction velocity and feed the SuOx-like activity, while the generation of hot carriers which are suggested as predominant effects catalyzes the oxidation of sulfite to sulfate through significantly decreasing the activation energy for the SuOx-like reaction. These investigations present a contribution to the basic understanding of plasmon-enhanced enzyme-like reaction and provided an insight into the optimization of the SuOx mimetic performance of nanomaterials.