RESUMEN
Metabolomics, the systematic study of metabolites, is dedicated to a comprehensive analysis of all aspects of plant-based food research and plays a pivotal role in the nutritional composition and quality control of plant-based foods. The diverse chemical compositions of plant-based foods lead to variations in sensory characteristics and nutritional value. This review explores the application of the metabolomics method to plant-based food origin tracing, cultivar identification, and processing methods. It also addresses the challenges encountered and outlines future directions. Typically, when combined with other omics or techniques, synergistic and complementary information is uncovered, enhancing the classification and prediction capabilities of models. Future research should aim to evaluate all factors affecting food quality comprehensively, and this necessitates advanced research into influence mechanisms, metabolic pathways, and gene expression.
Asunto(s)
Metabolómica , Plantas Comestibles/química , Plantas Comestibles/metabolismo , Plantas Comestibles/genética , Análisis de los Alimentos , Manipulación de Alimentos , Plantas/metabolismo , Plantas/química , Plantas/clasificaciónRESUMEN
Testicular dysfunction is a prevalent health problem frequently reported in individuals with diabetes mellitus (DM). Oxidative-inflammatory reactions, hormonal and spermatic abnormalities often accompany this illness. Herbal remedies "particularly wild plants" including chicory (Chicorium Intybus) and purslane (Portulaca Oleracea) are emerging as popular agents for people dealing with these issues due to their ability to act as antioxidants, reduce inflammation, and exhibit antidiabetic effects. According to the collected data, the daily administration of chicory (Ch) seed-extract (250 mg/kg) or purslane (Pu) seed-extract (200 mg/kg) to streptozotocin (STZ)-induced diabetic rats (50 mg/kg) for 30 days resulted in the normalization of fasting blood glucose (FBG), serum fructosamine, insulin levels, and insulin resistance (HOMA-IR), as well as reducing lipid peroxidation end-product malondialdehyde (MDA) level, aldehyde oxidase (AO) and xanthene oxidase (XO) activities. While caused a considerable improvement in glutathione (GSH) content, superoxide dismutase (SOD), catalase (CAT) activity, and total antioxidant capacity (TAC) when compared to diabetic rats. Ch and Pu extracts had a substantial impact on testicular parameters including sperm characterization, testosterone level, vimentin expression along with improvements in body and testis weight. They also mitigated hyperlipidemia by reducing total lipids (TL), total cholesterol (TC) levels, and low-density lipoprotein cholesterol (LDL-C), while increasing high-density lipoprotein cholesterol (HDL-C). Furthermore, oral administration of either Ch or Pu notably attuned the elevated proinflammatory cytokines as tumor necrotic factor (TNF-α), C-reactive protein (CRP), and Interleukin-6 (IL-6) together with reducing apoptosis and DNA damage. This was achieved through the suppression of DNA-fragmentation marker 8OHdG, triggering of caspase-3 immuno-expression, and elevation of Bcl-2 protein. The histological studies provided evidence supporting the preventive effects of Ch and Pu against DM-induced testicular dysfunction. In conclusion, Ch and Pu seed-extracts mitigate testicular impairment during DM due to their antihyperglycemic, antilipidemic, antioxidant, anti-inflammatory, and antiapoptotic properties.
Asunto(s)
Cichorium intybus , Diabetes Mellitus Experimental , Resistencia a la Insulina , Portulaca , Enfermedades Testiculares , Humanos , Ratas , Masculino , Animales , Portulaca/metabolismo , Antioxidantes/farmacología , Antioxidantes/uso terapéutico , Antioxidantes/metabolismo , 8-Hidroxi-2'-Desoxicoguanosina/metabolismo , Diabetes Mellitus Experimental/metabolismo , Plantas Comestibles/metabolismo , Glucemia/metabolismo , Extractos Vegetales/farmacología , Extractos Vegetales/uso terapéutico , Estrés Oxidativo , Hipoglucemiantes/farmacología , Hipoglucemiantes/uso terapéutico , Inflamación , Enfermedades Testiculares/tratamiento farmacológico , Glutatión/metabolismo , Colesterol/farmacologíaRESUMEN
One of the most pressing environmental issues is how to properly dispose of municipal solid waste (MSW), which represents both a substantial source of concern and a challenge. The current study evaluated cobalt (Co) accumulation in MSW, their uptake by different vegetables grown for two years, and related human health risks. Vegetables were grown in four different groups, such as one control (ground soil), and the remaining treatment groups (T1, T2, and T3) received varying concentrations of MSW. The analysis of Co was done through an atomic absorption spectrophotometer (AAS). Results revealed that the concentration of Co was higher in all the vegetables (n = 15) grown in soil supplemented with 75% MSW during 2nd growing year. Among all vegetables, the highest concentration of Co was observed in Solanum tuberosum at T3 during 2nd growing year. The pollution load index (PLI) value for vegetables during both growing years was more than 1 except in control soil. The findings indicated that the highest enrichment factor (EF) and hazard resilience index (HRI) value of 0.09 was present in S. tuberosum. Health index values for cobalt in the study were below 1. The HRI < 1 indicated that consumers do not face any immediate health risks. The investigation of Co concentrations in blood samples obtained from individuals residing in different areas contributes a human health perspective to the research. The findings indicate that the concentration of Co rises with an increasing proportion of MSW. While the metal levels in MSW-treated soil were not high enough to classify the soil as polluted, the results recommend that recycling MSW can substitute mineral fertilizers. Nevertheless, the presence of cobalt in MSW may directly affect soil fertility and could impact crop production and human health.
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Cobalto , Suelo , Residuos Sólidos , Cobalto/análisis , Humanos , Suelo/química , Residuos Sólidos/análisis , Compostaje , Salud Pública , Contaminantes del Suelo/análisis , Verduras/química , Verduras/metabolismo , Plantas Comestibles/química , Plantas Comestibles/metabolismoRESUMEN
Kitul (Caryota urens L.) inflorescences are broadly used for sweet sap production in Asian countries and Kitul food products are known as being suitable for diabetic patients. Considering the strong ability to inhibit α-glucosidase, we hypothesize that kitul antidiabetic properties might also involve the modulation of inflammatory pathways and hyperglycaemia-induced oxidative damage. Hence, the effects of an inflorescence's methanol extract were investigated in glucose-stimulated pancreatic cells (RIN-5F) and LPS-stimulated RAW 264.7 macrophages. The extract reduced the overproduction of intracellular reactive species in pancreatic cells and also NO, L-citrulline and IL-6 levels in LPS-stimulated RAW 264.7 macrophages. Inhibition of 5-lipoxygenase (IC50 = 166.1 µg/mL) through an uncompetitive manner was also recorded upon treatment with C. urens inflorescences extract. The phenolic profile of the inflorescences was characterized by HPLC-DAD, six hydroxycinnamic acids being identified and quantified. Overall, our data provide additional evidence on the pleiotropic mechanisms of Kitul inflorescences as an antidiabetic agent.
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Glucosa , Extractos Vegetales , Humanos , Ratones , Animales , Células RAW 264.7 , Extractos Vegetales/farmacología , Extractos Vegetales/metabolismo , Glucosa/metabolismo , Hipoglucemiantes/farmacología , Hipoglucemiantes/metabolismo , Mediadores de Inflamación/metabolismo , Lipopolisacáridos/farmacología , Antiinflamatorios/farmacología , Antiinflamatorios/metabolismo , Macrófagos , Plantas Comestibles/metabolismoRESUMEN
In contrast to traditional breeding, which relies on the identification of mutants, metabolic engineering provides a new platform to modify the oil composition in oil crops for improved nutrition. By altering endogenous genes involved in the biosynthesis pathways, it is possible to modify edible plant oils to increase the content of desired components or reduce the content of undesirable components. However, introduction of novel nutritional components such as omega-3 long-chain polyunsaturated fatty acids needs transgenic expression of novel genes in crops. Despite formidable challenges, significant progress in engineering nutritionally improved edible plant oils has recently been achieved, with some commercial products now on the market.
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Ácidos Grasos Omega-3 , Plantas Comestibles , Plantas Modificadas Genéticamente/genética , Plantas Modificadas Genéticamente/metabolismo , Plantas Comestibles/genética , Plantas Comestibles/metabolismo , Aceites de Plantas , Ácidos Grasos Omega-3/metabolismo , Ingeniería Metabólica , Productos Agrícolas/genética , Productos Agrícolas/metabolismo , Semillas/genética , Semillas/metabolismoRESUMEN
Potentially toxic elements (PTEs) pose a great threat to ecosystems and long-term exposure causes adverse effects to wildlife and humans. Cadmium induces a variety of diseases including cancer, kidney dysfunction, bone lesions, anemia and hypertension. Here we review the ability of plants to accumulate cadmium from soil, air and water under different environmental conditions, focusing on absorption mechanisms and factors affecting these. Cadmium possess various transport mechanisms and pathways roughly divided into symplast and apoplast pathway. Excessive cadmium concentrations in the environment affects soil properties, pH and microorganism composition and function and thereby plant uptake. At the same time, plants resist cadmium toxicity by antioxidant reaction. The differences in cadmium absorption capacity of plants need more exploration to determine whether it is beneficial for crop breeding or genetic modification. Identify whether plants have the potential to become hyperaccumulator and avoid excessive cadmium uptake by edible plants. The use of activators such as wood vinegar, GLDA (Glutamic acid diacetic acid), or the placement of earthworms and fungi can speed up phytoremediation of plants, thereby reducing uptake of crop varieties and reducing human exposure, thus accelerating food safety and the health of the planet.
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Cadmio , Contaminantes del Suelo , Humanos , Cadmio/análisis , Biodegradación Ambiental , Suelo/química , Ecosistema , Agua , Contaminantes del Suelo/análisis , Fitomejoramiento , Plantas Comestibles/metabolismoRESUMEN
Globally, several hundred thousand hectares of both agricultural and urban land have become contaminated with per- and polyfluoroalkyl substances (PFAS). PFAS compounds are resistant to degradation and are mobile in soil compared to other common contaminants. Many compounds have KD values (matrix/solution concentration quotients) of <10. PFAS compounds endanger the health of humans and ecosystems by leaching into groundwater, exposure via dust, and, to a lesser extent, through plant uptake. This review aims to determine the feasibility of phytomanagement, the use of plants, and the use of soil conditioners to minimize environmental risk whilst also providing an economic return in the management of PFAS-contaminated land. For most sites, PFAS combinations render phytoextraction, the use of plants to remove PFAS from soil, inviable. In contrast, low Bioaccumulation Coefficients (BAC; plant and soil concentration quotients) timber species or native vegetation may be usefully employed for phytomanagement to limit human/food chain exposure to PFAS. Even with a low BAC, PFAS uptake by crop plants may still exceed food safety standards, and therefore, edible crop plants should be avoided. Despite this limitation, phytomanagement may be the only economically viable option to manage most of this land. Plant species and soil amendments should be chosen with the goal of reducing water flux through the soil, as well as increasing the hydrophobic components in soil that may bind the C-F-dominated tails of PFAS compounds. Soil conditioners such as biochar, with significant hydrophobic components, may mitigate the leaching of PFAS into receiving waters. Future work should focus on the interactions of PFAS with soil microbiota; secondary metabolites such as glomalin may immobilize PFAS in soil.
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Fluorocarburos , Contaminantes del Suelo , Contaminantes Químicos del Agua , Ecosistema , Fluorocarburos/análisis , Humanos , Plantas Comestibles/química , Plantas Comestibles/metabolismo , Suelo/química , Contaminantes del Suelo/análisis , Contaminantes Químicos del Agua/análisisRESUMEN
BACKGROUND: Ice plant is a halophyte, known for its antioxidant activity and for being a highly functional food. It is capable of increasing its contents of health-promoting compounds when subjected to certain stresses such as salinity. The objective of this work was to determine the plant's best growing conditions to achieve both an optimal production of bioactive metabolites and high crop yield. Mesembryanthemum crystallinum were grown under semi-controlled conditions and four saline treatments were applied at: 0, 100, 200 and 300 mmol L-1 sodium chloride (NaCl), respectively. RESULTS: The 100 mmol L-1 NaCl treatment induced a slight increase in shoot dry weight (DW) and enhanced the leaf area. At higher salinity levels, however, the shoot biomass decreased. The concentration of starch and total proteins declined as the concentration of salt increased, while the total soluble sugars (TSS) content was lower in 100 and 300 mmol L-1 NaCl treatments. Proline increased in conditions over 100 mmol L-1 NaCl. Furthermore, plants grown with 300 mmol L-1 of NaCl presented the highest values of glutathione, ascorbic acid and vitamin C. Antioxidant enzymes activity and total phenolics increased with the severity of the salinity. CONCLUSION: Ice plant accumulates high levels of health-promoting compounds when grown with 300 mmol L-1 NaCl. A high concentration of beneficial compounds, however, is detrimental to the plant's growth. Moreover, 100 mmol L-1 NaCl treatment not only improved the concentration of bioactive and antioxidant compounds but also preserved the crop yield. It could thus be interesting to promote the cultivation of this high nutritional value plant in environments of moderate salinity. © 2021 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Asunto(s)
Mesembryanthemum , Antioxidantes/análisis , Ácido Ascórbico/metabolismo , Mesembryanthemum/metabolismo , Hojas de la Planta/química , Plantas Comestibles/metabolismo , Salinidad , Plantas Tolerantes a la Sal/metabolismo , Cloruro de Sodio/metabolismoRESUMEN
Specialized metabolites from plants are important for human health due to their antioxidant properties. Light is one of the main factors modulating the biosynthesis of specialized metabolites, determining the cascade response activated by photoreceptors and the consequent modulation of expressed genes and biosynthetic pathways. Recent developments in light emitting diode (LED) technology have enabled improvements in artificial light applications for horticulture. In particular, the possibility to select specific spectral light compositions, intensities and photoperiods has been associated with altered metabolite content in a variety of crops. This review aims to analyze the effects of indoor LED lighting recipes and management on the specialized metabolite content in different groups of crop plants (namely medicinal and aromatic plants, microgreens and edible flowers), focusing on the literature from the last 5 years. The literature collection produced a total of 40 papers, which were analyzed according to the effects of artificial LED lighting on the content of anthocyanins, carotenoids, phenols, tocopherols, glycosides, and terpenes, and ranked on a scale of 1 to 3. Most studies applied a combination of red and blue light (22%) or monochromatic blue (23%), with a 16 h day-1 photoperiod (78%) and an intensity greater than 200 µmol m-2 s-1 (77%). These treatment features were often the most efficient in enhancing specialized metabolite content, although large variations in performance were observed, according to the species considered and the compound analyzed. The review aims to provide valuable indications for the definition of the most promising spectral components toward the achievement of nutrient-rich indoor-grown products. © 2021 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
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Flores/química , Hojas de la Planta/química , Plantas Comestibles/metabolismo , Plantas Medicinales/metabolismo , Verduras/efectos de la radiación , Carotenoides/química , Carotenoides/metabolismo , Producción de Cultivos/instrumentación , Producción de Cultivos/métodos , Flores/crecimiento & desarrollo , Flores/metabolismo , Flores/efectos de la radiación , Luz , Fenoles/química , Fenoles/metabolismo , Hojas de la Planta/crecimiento & desarrollo , Hojas de la Planta/metabolismo , Hojas de la Planta/efectos de la radiación , Plantas Comestibles/química , Plantas Comestibles/crecimiento & desarrollo , Plantas Comestibles/efectos de la radiación , Plantas Medicinales/química , Plantas Medicinales/crecimiento & desarrollo , Plantas Medicinales/efectos de la radiación , Verduras/química , Verduras/crecimiento & desarrollo , Verduras/metabolismoRESUMEN
The consumption of plant sprouts as part of human day-to-day diets is gradually increasing, and their health benefit is attracting interest across multiple disciplines. The purpose of this review was to (a) critically evaluate the phytochemicals in selected sprouts (alfalfa, buckwheat, broccoli, and red cabbage), (b) describe the health benefits of sprouts, (c) assess the recent advances in sprout production, (d) rigorously evaluate their safety, and (e) suggest directions that merit special consideration for further novel research on sprouts. Young shoots are characterized by high levels of health-benefitting phytochemicals. Their utility as functional ingredients have been extensively described. Tremendous advances in the production and safety of sprouts have been made over the recent past and numerous reports have appeared in mainstream scientific journals describing their nutritional and medicinal properties. However, subjects such as application of sprouted seed flours in processed products, utilizing sprouts as leads in the synthesis of nanoparticles, and assessing the dynamics of a relationship between sprouts and gut health require special attention for future clinical exploration. Sprouting is an effective strategy allowing manipulation of phytochemicals in seeds to improve their health benefits.
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Dieta Saludable/tendencias , Valor Nutritivo , Fitoquímicos/análisis , Plantas Comestibles/crecimiento & desarrollo , Plantas Modificadas Genéticamente/crecimiento & desarrollo , Plantones/crecimiento & desarrollo , Semillas/crecimiento & desarrollo , Animales , Seguridad de Productos para el Consumidor , Manipulación de Alimentos , Germinación , Humanos , Plantas Comestibles/efectos adversos , Plantas Comestibles/genética , Plantas Comestibles/metabolismo , Plantas Modificadas Genéticamente/efectos adversos , Plantas Modificadas Genéticamente/genética , Plantas Modificadas Genéticamente/metabolismo , Plantones/efectos adversos , Plantones/genética , Plantones/metabolismo , Semillas/efectos adversos , Semillas/genética , Semillas/metabolismoRESUMEN
Indigenous communities across the globe, especially in rural areas, consume locally available plants known as Traditional Food Plants (TFPs) for their nutritional and health-related needs. Recent research shows that many TFPs are highly nutritious as they contain health beneficial metabolites, vitamins, mineral elements and other nutrients. Excessive reliance on the mainstream staple crops has its own disadvantages. Traditional food plants are nowadays considered important crops of the future and can act as supplementary foods for the burgeoning global population. They can also act as emergency foods in situations such as COVID-19 and in times of other pandemics. The current situation necessitates locally available alternative nutritious TFPs for sustainable food production. To increase the cultivation or improve the traits in TFPs, it is essential to understand the molecular basis of the genes that regulate some important traits such as nutritional components and resilience to biotic and abiotic stresses. The integrated use of modern omics and gene editing technologies provide great opportunities to better understand the genetic and molecular basis of superior nutrient content, climate-resilient traits and adaptation to local agroclimatic zones. Recently, realizing the importance and benefits of TFPs, scientists have shown interest in the prospection and sequencing of TFPs for their improvements, cultivation and mainstreaming. Integrated omics such as genomics, transcriptomics, proteomics, metabolomics and ionomics are successfully used in plants and have provided a comprehensive understanding of gene-protein-metabolite networks. Combined use of omics and editing tools has led to successful editing of beneficial traits in several TFPs. This suggests that there is ample scope for improvement of TFPs for sustainable food production. In this article, we highlight the importance, scope and progress towards improvement of TFPs for valuable traits by integrated use of omics and gene editing techniques.
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Seguridad Alimentaria/métodos , Plantas Comestibles/genética , Plantas Comestibles/metabolismo , Edición Génica , Genómica/métodos , Humanos , Metabolómica , Plantas Comestibles/química , ProteómicaRESUMEN
Rare earth elements (REEs) are emerging as a serious threat to ecological safety due to their increasing accumulation in environments. The accumulation of REEs in environments has significantly increased its accumulation in the leaves of edible plants. However, the accumulation pathway of REEs in the leaves of edible plants are still unknown. In this study, lanthanum [La(III), a widely used and accumulated REE] and four edible plants (soybean, lettuce, pakchoi, and celery) with short growth cycles were selected as research objects. By using interdisciplinary research techniques, we found that low-dose La(III) activated endocytosis (mainly the clathrin-mediated endocytosis) in the leaf cells of four edible plants, which provided an accumulation pathway for low-dose La in the leaf cells of these edible plants. The accumulation of La in the leaf cells was positively correlated with the intensity of endocytosis, while the intensity of endocytosis was negatively correlated with the density of leaf trichomes. In addition to the accumulation of La, low-dose La(III) also brought other risks. For example, the harmful element (Pb) can also be accumulated in the leaf cells via La(III)-activated endocytosis; the homeostasis of the essential elements (K, Ca, Fe, Mg) was disrupted, although the chlorophyll synthesis and the growth of these leaf cells were accelerated; and the expression of stress response genes (GmNAC20, GmNAC11) in soybean leaves was increased. These results provided an insight to further analyze the toxicity and mechanism of REEs in plants, and sounded the alarm for the application of REEs in agriculture.
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Endocitosis/efectos de los fármacos , Homeostasis/efectos de los fármacos , Lantano/metabolismo , Lantano/toxicidad , Plomo/metabolismo , Hojas de la Planta/efectos de los fármacos , Plantas Comestibles/efectos de los fármacos , Agricultura , Metales de Tierras Raras/metabolismo , Metales de Tierras Raras/toxicidad , Hojas de la Planta/metabolismo , Plantas Comestibles/metabolismo , Oligoelementos/metabolismoRESUMEN
Ready-to-use therapeutic food (RUTF) with adequate quality protein is used to treat children with oedematous and non-oedematous severe acute malnutrition (SAM). The plasma amino acid (AA) profile reflects the protein nutritional status; hence, its assessment during SAM treatment is useful in evaluating AA delivery from RUTFs. The objective was to evaluate the plasma AAs during the treatment of oedematous and non-oedematous SAM in community-based management of acute malnutrition (CMAM) using amino acid-enriched plant-based RUTFs with 10% milk (MSMS-RUTF) or without milk (FSMS-RUTF) compared to peanut milk RUTF (PM-RUTF). Plasma AA was measured in a non-blinded, 3-arm, parallel-group, simple randomized controlled trial conducted in Malawi. The RUTFs used for SAM were FSMS-RUTF, MSMS-RUTF or PM-RUTF. A non-inferiority hypothesis was tested to compare plasma AA levels from patients treated with FSMS-RUTF or MSMS-RUTF with those from patients treated with PM-RUTF at discharge. For both types of SAM, FSMS-RUTF and MSMS-RUTF treatments were non-inferior to the PM-RUTF treatment in restoration of the EAA and cystine except that for FSMS-RUTF, methionine and tryptophan partially satisfied the non-inferiority criteria in the oedematous group. Amino-acid-enriched milk-free plant-source-protein RUTF has the potential to restore all the EAA, but it is possible that enrichment with amino acids may require more methionine and tryptophan for oedematous children.
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Aminoácidos/metabolismo , Leche/metabolismo , Plantas Comestibles/metabolismo , Desnutrición Aguda Severa/dietoterapia , Animales , Arachis/metabolismo , Preescolar , Fabaceae/metabolismo , Femenino , Alimentos Fortificados/análisis , Humanos , Lactante , Malaui/epidemiología , Masculino , Leche/química , Proteínas de la Leche/metabolismo , Desnutrición Aguda Severa/epidemiología , Desnutrición Aguda Severa/metabolismo , Desnutrición Aguda Severa/patología , Resultado del TratamientoRESUMEN
Dynamic changes in flavonoid, total phenol, and antioxidant potential in different Prunus humilis accessions during fruit development stages were studied in order to provide a reference for the optimum harvest time for flavonoid extraction. 'Nongda 4', 'Nongda 5', 'DS-1' and '02-16' were selected as plant materials to determine the content of flavonoid, total phenol and antioxidant indices during six fruit development stages. Changes in total flavonoid content (TFC) and total phenol content (TPC) in different accessions of P. humilis were slightly different depending on the development stage of P. humilis fruit. TFC and TPC in 'Nongda 5' fruit showed a trend of continuous decline. There was a small increase in TFC and TPC from the young fruit stage to the stone hardening stage, followed by a decreasing trend, and then to the lowest level at the ripening stage of 'Nongda 4', 'DS-1', and '02-16' fruits. The trend of antioxidant capacity (ABTS, FRAP, DPPH) with the TFC and TPC of P. humilis fruit was basically the same, and the correlation analysis results showed that the TFC of P. humilis fruit was positively correlated with the antioxidant indices (P<0.01). Catechin (CC), rutin (RT), and quercetin-7-O-ß-D-glucopyranoside (Q7G) were detected in all the fruit development stages of the four P. humilis fruits. Among them, catechin was the most abundant component, accounting for approximately 10%. Myricetin (MC) and quercetin (QC) were generally detected only in the early fruit development stage, but not in the later fruit development stage. Correlation analysis showed that the flavonoid components with TFC, TPC, and antioxidant indices differed between the different accessions. RT, CC, and liquiritigenin (LR) had a stronger correlation with TFC and antioxidant indices. Cyanidin-3-O-glucoside (C3G) was not detected until the coloring stage in two red P. humilis accessions ('Nongda 4' and 'DS-1'), and so it is better to choose a red P. humilis fruit to extract C3G at the ripening stage. Selecting an early stage of fruit development, especially the stone hardening stage, was important for extracting flavonoids, total phenols and other components. We believe that our results will provide basic information and reference for evaluation of fruit nutrition and health benefits, breeding of functional new varieties, and efficient utilization of P. humilis fruit.
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Prunus/crecimiento & desarrollo , Prunus/metabolismo , Antioxidantes/metabolismo , China , Producción de Cultivos , Medicamentos Herbarios Chinos/metabolismo , Flavonoides/metabolismo , Frutas/crecimiento & desarrollo , Frutas/metabolismo , Humanos , Fenoles/metabolismo , Fitomejoramiento , Plantas Comestibles/crecimiento & desarrollo , Plantas Comestibles/metabolismo , Análisis de Componente PrincipalRESUMEN
Caryota urens L. has long been valued as a traditional food, the edible fruits being eaten raw and the inflorescences commonly used on sweet sap and flour production. In the current work, the phenolic profile of methanol extracts obtained from the inflorescences and fruits was unveiled for the first time, nine caffeic acid derivatives being identified and quantified. Since kitul products have been reported for their antidiabetic properties, extracts radical scavenging activity and α-amylase, α-glucosidase and aldose reductase inhibitory activity were assessed. The inflorescences' extract was particularly active against yeast α-glucosidase (IC50 = 1.53 µg/mL), acting through a non-competitive inhibitory mechanism. This activity was also observed in enzyme-enriched homogenates obtained from human Caco-2 cells (IC50 = 64.75 µg/mL). Additionally, the extract obtained from the inflorescences showed no cytotoxicity on HepG2, AGS and Caco-2 cell lines. Our data suggest that C. urens inflorescences can support the development of new functional foods with α-glucosidase inhibitory activity.
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Frutas/metabolismo , Inflorescencia/metabolismo , Plantas Comestibles/metabolismo , Células CACO-2 , Ácidos Cafeicos , Inhibidores de Glicósido Hidrolasas/farmacología , Humanos , Hipoglucemiantes/farmacología , Fenoles/análisis , Extractos Vegetales/farmacología , alfa-Amilasas/antagonistas & inhibidores , alfa-Glucosidasas/metabolismoRESUMEN
The (tentative) identification of unknown drug-related phase II metabolites in plants upon drug uptake remains a challenging task despite improved analytical instrument performance. To broaden the knowledge of possible drug metabolization, a fast-screening approach for the tentative identification of drug-related phase II metabolites is presented in this work. Therefore, an in silico database for the three non-steroidal anti-inflammatory drugs (ketoprofen, mefenamic acid, and naproxen) and a sub-group of their theoretical phase II metabolites (based on combinations with glucose, glucuronic acid, and malonic acid) was created. Next, the theoretical exact masses (protonated species and ammonia adducts) were calculated and used as precursor ions in an autoMS/MS measurement method. The applicability of this workflow was tested on the example of eleven edible plants, which were hydroponically grown in solutions containing the respective drug at a concentration level of 20 mg/L. For the three drugs investigated this led to the tentative identification of 41 metabolites (some of them so far not described in this context), such as combinations of hydroxylated mefenamic acid with up to four glucose units or hydroxylated mefenamic acid with two glucose and three malonic acid units.
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Antiinflamatorios no Esteroideos , Cromatografía Líquida de Alta Presión/métodos , Espectrometría de Masas/métodos , Plantas Comestibles , Contaminantes Químicos del Agua , Riego Agrícola , Antiinflamatorios no Esteroideos/análisis , Antiinflamatorios no Esteroideos/química , Antiinflamatorios no Esteroideos/metabolismo , Hidroponía , Plantas Comestibles/química , Plantas Comestibles/metabolismo , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/química , Contaminantes Químicos del Agua/metabolismoRESUMEN
BACKGROUND: Angola has one of the highest annual under-five mortality rates in in the world and malnutrition poses a severe problem in the country. This study is the first to focus on the traditional knowledge of plants, foods, and treatments used by the local population in the province of Uíge to affect the quality and quantity of human breast milk, since decades of independence and civil war impeded ethnobotanical studies in this area. METHODS: This study was conducted in eight municipalities in the province of Uíge, Northern Angola in February and March 2018. In 265 semi-structured interviews, 360 informants in 40 rural villages were asked about plants, food, and treatments used to affect the quality and quantity of human breast milk. Additionally, information on child mortality and the duration of breastfeeding were collected. Whenever possible, plant specimens were collected for later identification. To determine the local importance of the collected plants, food, and treatments, the Relative Frequency of Citations was calculated. RESULTS: Most women reported to have no problems with their breast milk production. The duration of breastfeeding meets the recommendations of the World Health Organization (WHO). Across all use categories, 69 plants from 36 plant families, and 21 other foods and treatments could be identified. CONCLUSIONS: The study shows an overview of a variety of plants, foods, and treatments used by mothers as galactagogues, to "clean" or to reduce their breast milk and those which they avoided to use during the lactation period. There is great potential for further research into this traditional knowledge. Also, further analysis of some of the plants could be of interest.
Asunto(s)
Lactancia Materna/etnología , Leche Humana/química , Plantas Comestibles/metabolismo , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Angola/etnología , Lactancia Materna/psicología , Etnobotánica , Femenino , Humanos , Lactante , Mortalidad Infantil , Recién Nacido , Lactancia , Persona de Mediana Edad , Leche Humana/metabolismo , Madres/psicología , Fitoterapia , Plantas Comestibles/clasificación , Plantas Medicinales/química , Población Rural/estadística & datos numéricos , Adulto JovenRESUMEN
Edible plant Jiaosu (EPJ), a type of plant-based functional food fermented by beneficial bacteria, has gained publicity in recent years for its potential benefits to health. Important progress in relevant manufacturing technology has been made in the past decade with respect to raw materials, fermentation microorganisms and fermentation conditions. Current research has revealed that EPJ contains abundant nutrients and bioactive compounds, such as minerals, amino acids, polyphenols, organic acids and polysaccharides. Thus, many studies have focused on the beneficial effects of EPJ in preventing lifestyle diseases, such as hyperglycemia, hyperlipidemia, non-alcoholic fatty liver, obesity, diabetes and some cancers, although limited studies have involved the related active compounds and their protective mechanisms. Furthermore, very few studies have investigated the potential safety risks associated with the consumption of such food. In this review, we present a brief summary of the current research progress pertaining to the manufacturing, bioactive compounds, potential health benefits and safety aspects of EPJ. However, as a result of the complex components in EPJ, further studies on the bioactive compounds with relevant beneficial effects in EPJ and the safety evaluations of EPJ consumption are needed. © 2020 Society of Chemical Industry.
Asunto(s)
Alimentos Fermentados/análisis , Plantas Comestibles/química , Plantas Comestibles/metabolismo , Bacterias/metabolismo , Alimentos Fermentados/microbiología , Microbiología de Alimentos , Inocuidad de los Alimentos , Salud , Humanos , Valor Nutritivo , Plantas Comestibles/microbiologíaRESUMEN
In this study, the inhibitory potentials of food originated 34 phenolic acids, and flavonoid compounds were screened against acetylcholinesterase, butyrylcholinesterase, urease, and tyrosinase enzymes. All compounds included in this study exhibited high antioxidant activity with an ignorable cytotoxic activity. In general, they also showed poor anti-urease and anti-tyrosinase activities. Compounds in aglycone form (quercetin, myricetin, chrysin, and luteolin) showed strong anticholinesterase activities. No relation was observed between the tested bioactivities except from the case that aglycone compounds exhibited a strong positive relationship between antioxidant activities and anticholinesterase activity. Interestingly, there was a relation between the molecular weights of aglycone compounds and their anticholinesterase activities. The study showed that flavonoids with molecular mass of 250-320 g/mol have high potential of anticholinesterase activities and are valuable for future experiments on animals and humans. Potential inhibitory effects of these molecules on target proteins were investigated using docking and molecular dynamics calculations.
Asunto(s)
Inhibidores de la Colinesterasa/química , Flavonoides/química , Hidroxibenzoatos/química , Plantas Comestibles/química , Acetilcolinesterasa/química , Acetilcolinesterasa/metabolismo , Animales , Antioxidantes/química , Sitios de Unión , Dominio Catalítico , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Inhibidores de la Colinesterasa/metabolismo , Inhibidores de la Colinesterasa/farmacología , Flavonoides/metabolismo , Flavonoides/farmacología , Humanos , Hidroxibenzoatos/metabolismo , Simulación del Acoplamiento Molecular , Monofenol Monooxigenasa/antagonistas & inhibidores , Monofenol Monooxigenasa/metabolismo , Estrés Oxidativo/efectos de los fármacos , Extractos Vegetales/química , Plantas Comestibles/metabolismoRESUMEN
Heavy metal accumulation in edible plants grown in contaminated soils poses a major environmental risk to humans and grazing animals. This study focused on the concentration and speciation of Zn in different edible plants grown in soils contaminated with smelter wastes (Spelter, WV, USA) containing high levels of the metals Zn, Cu, Pb, Cd. Their accumulation was examined in different parts (roots, stem, and leaves) of plants and as a function of growth stage (dry seed, sprouting seed, cotyledon, and leaves) in the root vegetables radish, the leafy vegetable spinach and the legume clover. Although the accumulation of metals varied significantly with plant species, the average metal concentrations were [Zn] > [Pb] > [Cu] > [Cd]. Metal uptake studies were complemented with bulk and micro X-ray absorption spectroscopy (XAS) at Zn K-edge and micro X-ray fluorescence (µXRF) measurements to evaluate the speciation and distribution of Zn in these plant species. Dynamic interplay between the histidine and malate complexation of Zn was observed in all plant species. XRF mapping of spinach leaves at micron spatial resolution demonstrated the accumulation of Zn in vacuoles and leaf tips. Radish root showed accumulation of Zn in root hairs, likely as ZnS nanoparticles. At locations of high Zn concentration in spinach leaves, µXANES suggests Zn complexation with histidine, as opposed to malate in the bulk leaf. These findings shed new light on the dynamic nature of Zn speciation in plants.