Critical evaluation of third-order advantage with highly overlapped spectral signals. Determination of fluoroquinolones in fish-farming waters by fluorescence spectroscopy coupled to multivariate calibration.

In this work, the analytical performance of a third-order/four-way calibration is evaluated to model a highly overlapped system, where two spectral dimensions are extremely similar, and the results are then compared with the results of second-order/three-way calibration. The four-way data were obtained during the photodegradation of fluoroquinolones (ciprofloxacin, norfloxacin and flumequine) in the form of excitation-emission matrices and modeled by unfolded partial least squares coupled to residual trilinearization (U-PLS-RTL). According to the results, the model obtained with the second-order algorithm (unfolded partial least squares coupled to residual bilinearization: U-PLS-RBL) was unsatisfactory due to high spectral overlap. The third-order approach obtained a satisfactory fit and better figures of merit (LOD, REP, RMSEP, and sensitivity, among others) even in the presence of unexpected interferences due to third-order advantages. Finally, the analytical method based on third-order multivariate calibration was applied to quantify these fluoroquinolones in spiked fish-farming water samples. In this case, the third-order advantage allowed us to satisfactorily model the data and to quantify these compounds in this complex matrix, demonstrating the superior analytical performance of the high-order data that were evaluated.

Trace elements and microbiological parameters in farmed Nile tilapia with emphasis on muscle, water, sediment and feed.

Fish may bioaccumulate contaminants from the aquatic environment and extend them to the food chain provoking risks to human health. This study evaluated the microbiological parameters of the pond´s water and trace elements concentrations in samples of water, sediment, feed and muscle of farmed Nile tilapia used for human consumption in southern Brazil. A total of 240 fish were collected from 12 tilapia farms. Sediment, tank water and dry ration used in the animals' diet were collected for analysis. Analysis were performed by Energy Dispersion X-ray Fluorescence (EDXRF), Inductively Coupled Plasma Mass Spectrometer (ICP-MS), Induced Coupled Plasma Optical Emission Spectrometer (ICP-OES), and Atomic Absorption Spectrometer (AAS-VGA). In addition, the microbiological analysis of the water was carried out. The concentrations of Se, I, Fe, Cu and Zn in fish muscle were higher than the recommended by the Brazilian legislation, considering the advised daily intake for adults. The arsenic element had concentrations above the limit stipulated by the present Brazilian legislation, observed in all samples of muscle, sediment and tank water highlighting a possible environmental and fish contamination by the toxic element. Moreover, the arsenic concentration in the water presented a positive correlation (ρ = 0.33) with arsenic in the muscle, suggesting that tilapia is a good environmental bioindicator, once they properly reflect the levels of arsenic in the water. It is suggested to perform an arsenic speciation for quantification of the inorganic form and accurate assessment of the degree of toxicity in the muscle samples and risks it can bring to human health. Regarding the other potentially toxic elements (Hg, Pb and Cd), and microbiological analysis of water it was verified that the consumption of the fish in question does not raise risks, since the values are within a quality benchmark established by law. The concentration of total and fecal coliforms in pond´s water in the facilities was in agreement with the microbiological indexes required by the legislation of CONAMA class II. Western region presented the lowest concentrations of fecal coliforms when compared to the other regions. There was no significant difference in the microbiological counts of total heterotrophic bacteria, Vibrio spp. and Pseudomonas spp. among the regions.

Ausência de ictioparasitofauna em lagoa natural do município de Ângulo - Paraná / Absence of ictioparasitofauna in natural lagoon in the municipality of Ângulo - Paraná

Ictioparasitofauna de um ambiente permite avaliar a qualidade da água, o estado imunofisiológico dos organismos presentes no ambiente. Grande número de peixes possuem qualidades de adaptação que os permitem sobreviver em ambientes desfavoráveis. Já os organismos parasitos, são mais sensíveis, e desaparecem dos hospedeiros diante de mudanças ambientes, principalmente quando se trata de agrotóxicos, ou resíduos químicos. Com o crescimento da produção de cana-de-açúcar aumenta a quantidade de agrotóxicos e poluição ambiental. O objetivo desse trabalho foi avaliar a fauna parasitária de peixes nativos e introduzidos em lagoa natural no município de Ângulo no Paraná. A lagoa é circundada por cultivo de cana-de-açúcar. A coleta dos peixes ocorreu no período entre Outono e Inverno, esses animais foram pescados e encaminhados para o laboratório de Parasitologia da Uningá. Foram coletados nove peixes de diferentes espécies, entre eles um Brycon amazonicus, cinco Astyanax altiparanae, um Piaractus mesopotamicus, ambos peixes nativos, um Oreochromis niloticus e um Arius africanus que são exóticos. O único parasito encontrado foi um Copepoda, Ergasilus sp. em um Astyanax altiparanae. Devido à baixa quantidade de parasitos a infestação pode ser considerada acidental.(AU)

Ictioparasitofauna of an environment allows evaluating the water quality, the quality and the immunophysiological state of the aquatic organisms present in specific environments. Large numbers of fish have adaptive qualities in their physiological and behavioral state, which allow them to survive in unfavorable environments. On the other hand, the parasite organisms are more sensitive, and disappear from the hosts in the face of environmental changes, especially when dealing with pesticides or chemical residues. With the growth of sugarcane production in the state of Paraná, also increases the amount of pesticides and environmental pollution. The objective of this work was to evaluate the parasitic fauna of native fish and introduced in a natural lagoon in the municipality of Angulo in Paraná, southern Brazil. The lagoon is surrounded by cultivation of sugar cane. The fish were collected between autumn and winter, these animals were fished with rod and hook, donated, already killed by the fisherman, and sent to the laboratory of Parasitology of the Uningá Veterinary Hospital. Nine fish of different species were collected, including 1 Brycon amazonicus, 5 Astyanax altiparanae, 1 Piaractus mesopotamicus, both native fish, 1 Oreochromis niloticus and 1 Arius africanus that are exotic. The only parasite found was a Copepoda Crustacean, Ergasilus sp., established in an Astyanax altiparanae, due to the low amount of parasites the infestation could be considered accidental.(AU)

Ausência de ictioparasitofauna em lagoa natural do município de Ângulo - Paraná / Absence of ictioparasitofauna in natural lagoon in the municipality of Ângulo - Paraná

Ictioparasitofauna de um ambiente permite avaliar a qualidade da água, o estado imunofisiológico dos organismos presentes no ambiente. Grande número de peixes possuem qualidades de adaptação que os permitem sobreviver em ambientes desfavoráveis. Já os organismos parasitos, são mais sensíveis, e desaparecem dos hospedeiros diante de mudanças ambientes, principalmente quando se trata de agrotóxicos, ou resíduos químicos. Com o crescimento da produção de cana-de-açúcar aumenta a quantidade de agrotóxicos e poluição ambiental. O objetivo desse trabalho foi avaliar a fauna parasitária de peixes nativos e introduzidos em lagoa natural no município de Ângulo no Paraná. A lagoa é circundada por cultivo de cana-de-açúcar. A coleta dos peixes ocorreu no período entre Outono e Inverno, esses animais foram pescados e encaminhados para o laboratório de Parasitologia da Uningá. Foram coletados nove peixes de diferentes espécies, entre eles um Brycon amazonicus, cinco Astyanax altiparanae, um Piaractus mesopotamicus, ambos peixes nativos, um Oreochromis niloticus e um Arius africanus que são exóticos. O único parasito encontrado foi um Copepoda, Ergasilus sp. em um Astyanax altiparanae. Devido à baixa quantidade de parasitos a infestação pode ser considerada acidental.

Ictioparasitofauna of an environment allows evaluating the water quality, the quality and the immunophysiological state of the aquatic organisms present in specific environments. Large numbers of fish have adaptive qualities in their physiological and behavioral state, which allow them to survive in unfavorable environments. On the other hand, the parasite organisms are more sensitive, and disappear from the hosts in the face of environmental changes, especially when dealing with pesticides or chemical residues. With the growth of sugarcane production in the state of Paraná, also increases the amount of pesticides and environmental pollution. The objective of this work was to evaluate the parasitic fauna of native fish and introduced in a natural lagoon in the municipality of Angulo in Paraná, southern Brazil. The lagoon is surrounded by cultivation of sugar cane. The fish were collected between autumn and winter, these animals were fished with rod and hook, donated, already killed by the fisherman, and sent to the laboratory of Parasitology of the Uningá Veterinary Hospital. Nine fish of different species were collected, including 1 Brycon amazonicus, 5 Astyanax altiparanae, 1 Piaractus mesopotamicus, both native fish, 1 Oreochromis niloticus and 1 Arius africanus that are exotic. The only parasite found was a Copepoda Crustacean, Ergasilus sp., established in an Astyanax altiparanae, due to the low amount of parasites the infestation could be considered accidental.

Monitoring the morphological integrity of neotropical anurans.

Amphibians are considered to be excellent bioindicators to their morphophysiological characteristics and life cycle. In this context, the present study investigated the morphological integrity of anuran larvae collected in preserved environments in the Emas National Park, in the municipality of Mineiros (Goiás state, Brazil), and in environments representative of the agricultural matrix of the Rio Verde region, also in Goiás, where there is a long history of the use of agricultural pesticides. Samples of water from temporary ponds, permanent dams, and veredas were analyzed for the presence of pesticides and, especially atrazine (5350 µg/L), found at significantly higher concentrations in the agricultural matrix. We observed a high percentage (approximately 10%) of morphological malformations including alterations of the fins in Boana albopunctatus and Scinax fuscovarius; alteration in oral structures in B. albopunctatus, Dematonotus muelleri, Physalaemus centralis, Physalaemus cuvieri, and Leptodactylus fuscus mainly in the tadpoles collected in the agricultural environment in comparison with those from the protected area (3.5%; P < 0.0001, χ2 31.75). However, changes in the eyes, mouth, intestines, and nostrils, as well as amelia were observed only in the agricultural environment. The vast majority of the observed malformations were associated with the tail and oral disc, which suggests that these anatomical parameters may be used as sensitive morphological biomarkers. Given these findings, we reinforce that areas of agricultural land may have a deleterious effect on the morphological integrity of the tadpoles and consequently, on their development, and that these features may be used as indicators of environmental quality and health.

Automatic feeders for Nile tilapia raised in cages: productive performance at high feeding frequencies and different rates / Alimentadores automáticos para tilápia-do-nilo criadas em tanques-rede: desempenho produtivo com alta frequência alimentar e diferentes taxas de alimentação

The main goal of modern intensive fish farms should be to increase productivity at a low cost. The automation of fish feeding plays an important role in this scenario since it may improve feed efficiency and reduce organic waste emissions. The aim of this experiment was to provide basic information for the development of feeding techniques using automatic feeders. The experiment was done from September to December, with tilapia weighing 185g, fed 48 meals a day at 22 or 30 minute intervals, with feeding rates of 2, 3 and 4% of live weight. Eighteen 1m3 net cages equipped with automatic feeders were distributed in one pond. During the experimental trial the mean values for dissolved oxygen, pH and temperature were 3.20mg/l, 8.03 and 25.43°C, respectively. The highest average weight of 683.73g was achieved for fish treated with 4% of live weight at 30 minute intervals between meals. There was no difference for viscero-somatic index, demonstrating no alteration in the final carcass quality. These results demonstrate that a higher feeding rate added to efficient feed management can be used at the final stages of raising Nile tilapia in cages without compromising final fish productive quality.(AU)

O principal objetivo da criação intensiva de peixes deve ser o aumento da produtividade com baixos custos. Automação da alimentação tem papel importante nesse cenário, uma vez que melhora a eficiência alimentar e reduz a emissão de lixo orgânico. O objetivo deste experimento foi produzir informação básica para o desenvolvimento de tecnologia alimentar mediante o uso de alimentadores automáticos. O experimento foi realizado de setembro a dezembro, com tilápias pesando 185g e alimentadas 48 vezes ao dia, em intervalos de 22 ou 30 minutos, com taxa de alimentação de 2, 3 e 4% do peso vivo. Dezoito tanques-rede de 1m3 foram equipados com alimentadores automáticos e distribuídos em um lago. Durante o experimento, a média de oxigênio dissolvido, a de pH e a de temperatura foram 3,20mg/L, 8.03 e 25,43°C, respectivamente. A maior média de peso de 683,73g foi obtida para os peixes tratados com 4% do peso vivo e intervalo de 30 minutos entre as refeições. Não houve diferença no índice víscero-somático, o que demonstra a mesma qualidade final da carcaça. Esses resultados mostram que a alta taxa de alimentação, associada ao eficiente manejo alimentar, pode ser usada nos estágios finais de produção de tilápia-do-nilo em tanques-rede, sem comprometer a qualidade do produto final.(AU)

Potential pest transfer mediated by international ornamental plant trade.

In recent years, the keeping of ornamental freshwater animals and plants in garden ponds has been growing in popularity. Water hyacinth (Eichhornia crassipes) is one of the preferred macrophytes seasonally imported mainly from South-eastern Asia throughout the world. This constitutes a secondary introduction inasmuch as the species is native to South America. Although many assemblages of aquatic invertebrates have been described as associated with this plant in the wild, there has been no research focused on their potential introduction via the international plant trade. We examined 216 specimens of water hyacinths imported for ornamental purposes from Indonesia into the Czech Republic. Numerous meio- and macroinvertebrates belonging to at least 39 species were captured. On the total number of individuals, the highest prevalence was of Tubulinea and Rotifera. Most of these were still alive and vital, including a caterpillar of the Indo-Australian invasive moth Spodoptera litura. Water hyacinths are usually placed into outdoor ponds immediately after import, which facilitates the release of non-target alien species. The present paper aims to draw attention to "hitchhikers" associated with the ornamental trade.

Dietary habits of juveniles of the Mayan cichlid, Cichlasoma urophthalmus, in mangrove ponds of an offshore islet in Belize, Central America

Foraging habitats of juveniles of the Mayan cichlid, Cichlasoma urophthalmus (Günther, 1862), were investigated in two mangrove ponds located in Twin Cays offshore islet in Belize: Sink Hole pond (SH) and Hidden Lake pond (HL). Sink Hole pond is a semiclosed body of water, whereas Hidden Lake pond is connected by a channel to adjacent seagrass beds that surround the islet. Gut contents of 21 juvenile C. urophthalmus (9.8-13.2 cm total length) were analyzed, and five prey taxa were identified. In both mangrove ponds, C. urophthalmus were opportunistic carnivores and consumed primarily crustaceans. Plant material and detritus present in gut contents were most likely ingested incidentally when the fish foraged on small invertebrates. Carbon isotopic values of fish specimens from the two ponds were similar (mean ± SD of -19.2 ± 0.4‰ in SH and -19.4 ± 0.4‰ in HL), and were close to those of mangrove prey (mean ± SD = -20.2 ± 1.5‰), suggesting that this fish species forages in this habitat. Mixing models showed a higher contribution of mangrove food sources to the fish diet than seagrass food sources. This study reveals that young Mayan cichlids, inhabiting two Belize mangrove ponds, are generalists and opportunistic carnivores that forage on mangrove food sources and do not appear to move to adjacent seagrass beds to complement their diets. Understanding trophic linkages between aquatic consumers and food resources may contribute to better management of threatened coastal ecosystems.

Habitats de alimentação de juvenis do ciclídeo-maia, Cichlasoma urophthalmus (Günther, 1862), foram investigados em duas lagoas de mangue localizadas nas ilhas Twin Cays em alto mar em Belize: Sink Hole Lake (SH) e Hidden Lake (HL). Sink Hole é um corpo d'água parcialmente isolado, enquanto Hidden Lake é ligada por um canal com bancos de sargaços que cercam a ilhota. O conteúdo estomacal de 21 juvenil de C. urophthalmus (9,8-13,2 cm de comprimento total) foram analisados e cinco táxons de presas foram identificados. Em ambas as lagoas de mangue, 'C.' urophthalmus foram carnívoros oportunistas e consumiram principalmente crustáceos. Material vegetal e detritos presentes no conteúdo digestivo foram provavelmente ingeridos acidentalmente quando o peixe se alimentava de pequenos invertebrados. Os valores de isótopos estáveis do carbono em espécimes de peixes das duas lagoas foram semelhantes (média ± SD -19,2 ± 0,4 ‰ em SH e -19,4 ± 0,4 ‰ em HL), e foram próximos aos de presas de mangue (mean ± SD = -20.2 ± 1.5 ‰), sugerindo que esta espécie de peixe vai à procura de alimentos neste habitat. Modelos mistos mostraram uma maior contribuição de fontes alimentares de mangue para a dieta dos peixes do que de fontes alimentares de algas marinhas. Este estudo revela que juvenis do ciclídeo-maia que habitam duas lagoas de mangue em Belize, são carnívoros generalistas e oportunistas que se ingerem alimentos dos manguezais e não parecem se mover para leitos de algas marinhas adjacentes para complementar suas dietas. Compreender as ligações tróficas entre consumidores aquáticos e recursos alimentares pode contribuir para uma melhor gestão dos ecossistemas costeiros ameaçados.

Dietary habits of juveniles of the Mayan cichlid, Cichlasoma urophthalmus, in mangrove ponds of an offshore islet in Belize, Central America

Foraging habitats of juveniles of the Mayan cichlid, Cichlasoma urophthalmus (Günther, 1862), were investigated in two mangrove ponds located in Twin Cays offshore islet in Belize: Sink Hole pond (SH) and Hidden Lake pond (HL). Sink Hole pond is a semiclosed body of water, whereas Hidden Lake pond is connected by a channel to adjacent seagrass beds that surround the islet. Gut contents of 21 juvenile C. urophthalmus (9.8-13.2 cm total length) were analyzed, and five prey taxa were identified. In both mangrove ponds, C. urophthalmus were opportunistic carnivores and consumed primarily crustaceans. Plant material and detritus present in gut contents were most likely ingested incidentally when the fish foraged on small invertebrates. Carbon isotopic values of fish specimens from the two ponds were similar (mean ± SD of -19.2 ± 0.4‰ in SH and -19.4 ± 0.4‰ in HL), and were close to those of mangrove prey (mean ± SD = -20.2 ± 1.5‰), suggesting that this fish species forages in this habitat. Mixing models showed a higher contribution of mangrove food sources to the fish diet than seagrass food sources. This study reveals that young Mayan cichlids, inhabiting two Belize mangrove ponds, are generalists and opportunistic carnivores that forage on mangrove food sources and do not appear to move to adjacent seagrass beds to complement their diets. Understanding trophic linkages between aquatic consumers and food resources may contribute to better management of threatened coastal ecosystems.(AU)

Habitats de alimentação de juvenis do ciclídeo-maia, Cichlasoma urophthalmus (Günther, 1862), foram investigados em duas lagoas de mangue localizadas nas ilhas Twin Cays em alto mar em Belize: Sink Hole Lake (SH) e Hidden Lake (HL). Sink Hole é um corpo d'água parcialmente isolado, enquanto Hidden Lake é ligada por um canal com bancos de sargaços que cercam a ilhota. O conteúdo estomacal de 21 juvenil de C. urophthalmus (9,8-13,2 cm de comprimento total) foram analisados e cinco táxons de presas foram identificados. Em ambas as lagoas de mangue, 'C.' urophthalmus foram carnívoros oportunistas e consumiram principalmente crustáceos. Material vegetal e detritos presentes no conteúdo digestivo foram provavelmente ingeridos acidentalmente quando o peixe se alimentava de pequenos invertebrados. Os valores de isótopos estáveis do carbono em espécimes de peixes das duas lagoas foram semelhantes (média ± SD -19,2 ± 0,4 ‰ em SH e -19,4 ± 0,4 ‰ em HL), e foram próximos aos de presas de mangue (mean ± SD = -20.2 ± 1.5 ‰), sugerindo que esta espécie de peixe vai à procura de alimentos neste habitat. Modelos mistos mostraram uma maior contribuição de fontes alimentares de mangue para a dieta dos peixes do que de fontes alimentares de algas marinhas. Este estudo revela que juvenis do ciclídeo-maia que habitam duas lagoas de mangue em Belize, são carnívoros generalistas e oportunistas que se ingerem alimentos dos manguezais e não parecem se mover para leitos de algas marinhas adjacentes para complementar suas dietas. Compreender as ligações tróficas entre consumidores aquáticos e recursos alimentares pode contribuir para uma melhor gestão dos ecossistemas costeiros ameaçados.(AU)

Diversidade e ocorrência de peixes na área de influência da UHE Mogi Guaçu e lagoas marginais, bacia do alto rio Paraná, São Paulo, Brasil / Fish diversity and occurrence in the influence area of the Mogi Guaçu reservoir and oxbow lakes, high Paraná river basin, São Paulo, Brazil

A bacia do alto Paraná apresenta os rios de maior porte do Estado de São Paulo e uma ictiofauna bastante diversa, com 236 espécies. Este estudo visou caracterizar a composição ictiofaunística do reservatório da UHE Mogi Guaçu e de um conjunto de lagoas marginais naturais a jusante da represa, de acordo com a variação temporal da abundância das espécies. Durante agosto de 2005 a julho de 2006, foram coletados 2.341 exemplares pertencentes a 41 espécies. Na represa foram registradas 31 espécies e nas lagoas marginais 24, revelando uma dominância de espécies de Curimatidae (Cyphocharax modestus e Steindachnerina insculpta) e Characidae (Hyphessobrycon eques e H. bifasciatus) e um número expressivo de espécies raras. As curvas cumulativas de abundância e biomassa indicaram que na represa as espécies foram dominantes em número e nas lagoas em biomassa, refletindo um ambiente sob ações antrópicas e outro menos perturbado, respectivamente. A represa e as lagoas marginais apresentaram valores próximos de diversidade e eqüitabilidade de Shannon. No geral, os maiores valores foram obtidos durante o período chuvoso e os menores durante o período seco, porém esta variação não foi suficiente para a ANOVA detectar diferenças significativas entre os ambientes, nem entre os períodos. A interpretação destes resultados deve ser feita com cautela, pois apesar dos índices de diversidade e eqüitabilidade de Shannon não terem evidenciado as diferenças na composição da ictiofauna, a análise de similaridade pelo índice de Morisita-Horn apontou que a ictiofauna da represa e das lagoas marginais apresentam baixa similaridade entre si. Além disso, a ictiofauna nos ambientes estudados foi composta por peixes típicos da bacia do alto Paraná indicando uma condição que, embora alterada pela construção da UHE, apresenta espécies essencialmente nativas desta região. Sugere-se que o controle da vazão do rio pela UHE Mogi Guaçu e a extração de areia do leito estejam influenciando...

The high Paraná river basin includes the largest rivers from São Paulo State and a great ichthyofauna diversity with 236 species. The aim of this study was to describe the fish fauna composition from Mogi Guaçu hydroelectric dam and oxbow lakes downstream reservoir, according to species abundance temporal variations. A total of 2.341 individuals (85,8 kg biomass) belonging to 41 species were collected during August 2005 to July 2006. From this, 31 species were found in the reservoir and 24 in the oxbow lakes showing the Curimatidae (Cyphocharax modestus and Steindachnerina insculpta) and Characidae (Hyphessobrycon eques and H. bifasciatus) dominance and many rare species. The abundance and biomass cumulative curves showed the numerical dominance in reservoir and biomass in oxbow lakes, reflecting an environment more and less disturbed, respectively. Similar values of Shannon's diversity and evenness were found to reservoir and oxbow lakes. In general, the highest values were obtained to the wet season and the smaller to the dry, but this variation were not sufficient to detect significant differences between areas, neither seasons. The species identities are not considered in these indexes computation, being insensitive to ichthyofauna composition differences between the reservoir and the oxbow lakes. The interpretation of these results should be taken carefully because despite the Shannon's indexes showed no differences in fish fauna composition between areas, the similarity analyses using the Morisita-Horn index showed low similarity among them. Moreover, the ichthyofauna found in the studied area was composed by typical species from high Paraná river basin which indicates a good condition with native species only, despite the impacts commonly caused by hydroelectric dams. We suggest that the dam regulation and the sand extraction from the river bed are affecting the flood pulses and consequently the oxbow lake's ichthyofauna.