RESUMEN
Considerable attention has been paid to the roles of lipid metabolism in virus infection due to its regulatory effects on virus replication and host antiviral immune response. However, few literature has focused on whether lipid metabolism is involved in the life cycle of lower vertebrate viruses. Singapore grouper iridovirus (SGIV) is the causative aquatic virus that extensively causes fry and adult groupers death. Here, the potential roles of cellular de novo fatty acid synthesis in SGIV infection was investigated. SGIV infection not only increased the expression levels of key enzymes in fatty acid synthesis in vivo/vitro, including acetyl-Coenzyme A carboxylase alpha (ACC1), fatty acid synthase (FASN), medium-chain acyl-CoA dehydrogenase (MCAD), adipose triglyceride lipase (ATGL), lipoprotein lipase (LPL) and sterol regulatory element-binding protein-1 (SREBP1), but it also induced the formation of lipid droplets (LDs), suggesting that SGIV altered de novo fatty acid synthesis in host cells. Using the inhibitor and specific siRNA of ACC1 and FASN, we found that fatty acid synthesis was essential for SGIV replication, evidenced by their inhibitory effects on CPE progression, viral gene transcription, protein expression and virus production. Moreover, the inhibitor of fatty acid ß-oxidation could also reduce SGIV replication. Inhibition of fatty acid synthesis but not ß-oxidation markedly blocked virus entry during the life cycle of SGIV infection. In addition, we also found that inhibition of ACC1 and FASN increased the IFN immune and inflammatory response during SGIV infection. Together, our data demonstrated that SGIV infection in vitro regulated host lipid metabolism and, in that process, cellular fatty acid synthesis might exert crucial roles during SGIV infection via regulating virus entry and host immune response.
Asunto(s)
Infecciones por Virus ADN/virología , Ácidos Grasos/metabolismo , Enfermedades de los Peces/virología , Interacciones Huésped-Patógeno , Metabolismo de los Lípidos , Ranavirus/fisiología , Acetiltransferasas/metabolismo , Acil-CoA Deshidrogenasa/metabolismo , Animales , Ácido Graso Sintasas/metabolismo , Regulación Enzimológica de la Expresión Génica , Inmunidad , Lipasa/metabolismo , Lipoproteína Lipasa/metabolismo , Perciformes , Ranavirus/enzimología , Bazo/virología , Proteína 1 de Unión a los Elementos Reguladores de Esteroles/metabolismo , Internalización del Virus , Replicación ViralRESUMEN
A survey was performed on ornamental fish imported into the EU to detect viral agents belonging to the genus Ranavirus. The objective was to gain knowledge of the potential for these systemic iridoviruses to gain entry into the EU via international trade in ornamental fish. A total of 208 pooled samples, representing 753 individual fish, were tested. The samples included 13 orders and 37 families, originating from different countries and continents. Tissues from fish that died during or just after transport were collected and examined by standard virological techniques in epithelioma papulosum cyprini cells, by transmission electron microscopy and by PCR for the detection of the major capsid protein and DNA polymerase gene sequences of ranaviruses. Virus was isolated from nine fish species but ranavirus was not identified in those samples. The results suggest that ranaviruses are not highly prevalent in ornamental fish imported into the EU.
Asunto(s)
Infecciones por Virus ADN/veterinaria , Enfermedades de los Peces/virología , Peces/virología , Ranavirus/genética , Animales , Proteínas de la Cápside/análisis , Proteínas de la Cápside/genética , Carcinoma/virología , Línea Celular/virología , Infecciones por Virus ADN/genética , ADN Polimerasa Dirigida por ADN/análisis , ADN Polimerasa Dirigida por ADN/genética , Unión Europea , Microscopía Electrónica de Transmisión , Filogenia , Reacción en Cadena de la Polimerasa , Ranavirus/clasificación , Ranavirus/enzimología , Ranavirus/ultraestructura , Proteínas Virales/análisis , Proteínas Virales/genéticaRESUMEN
Purine nucleoside phosphorylase (PNP) catalyzes the reversible phosphorolysis of purine ribonucleosides to the corresponding free bases and ribose 1-phosphate. The crystal structure of grouper iridovirus PNP (givPNP), corresponding to the first PNP gene to be found in a virus, was determined at 2.4 A resolution. The crystals belonged to space group R3, with unit-cell parameters a = 193.0, c = 105.6 A, and contained four protomers per asymmetric unit. The overall structure of givPNP shows high similarity to mammalian PNPs, having an alpha/beta structure with a nine-stranded mixed beta-barrel flanked by a total of nine alpha-helices. The predicted phosphate-binding and ribose-binding sites are occupied by a phosphate ion and a Tris molecule, respectively. The geometrical arrangement and hydrogen-bonding patterns of the phosphate-binding site are similar to those found in the human and bovine PNP structures. The enzymatic activity assay of givPNP on various substrates revealed that givPNP can only accept 6-oxopurine nucleosides as substrates, which is also suggested by its amino-acid composition and active-site architecture. All these results suggest that givPNP is a homologue of mammalian PNPs in terms of amino-acid sequence, molecular mass, substrate specificity and overall structure, as well as in the composition of the active site.
Asunto(s)
Purina-Nucleósido Fosforilasa/química , Ranavirus/enzimología , Secuencia de Aminoácidos , Animales , Sitios de Unión , Secuencia Conservada , Cristalografía por Rayos X , Humanos , Modelos Moleculares , Datos de Secuencia Molecular , Fosfatos/química , Fosfatos/metabolismo , Estructura Cuaternaria de Proteína , Estructura Terciaria de Proteína , Purina-Nucleósido Fosforilasa/genética , Purina-Nucleósido Fosforilasa/metabolismo , Ranavirus/genética , Alineación de Secuencia , Especificidad por SustratoRESUMEN
In this study, we developed new methods for differentiation of ranaviruses based on polymerase chain reaction and restriction enzyme analysis of DNA polymerase and neurofilament triplet H1-like (NF-H1) protein gene. Using these methods, we were able to differentiate the 6 known ranaviruses--Bohle iridovirus (BIV), European catfish virus (ECV), epizootic haematopoietic necrosis virus (EHNV), European sheatfish virus (ESV), frog virus 3 (FV3) and Singapore grouper iridovirus (SGIV)--with 3 less characterised virus isolates: short-finned eel ranavirus (SERV), Rana esculenta virus Italy 282/I02 (REV 282/I02) and pike-perch iridovirus (PPIV). Doctor fish virus (DFV) and guppy virus 6 (GV6) were distinguished as a group from the other viruses. In addition, all 11 isolates were analysed and compared based on nucleotide sequences from 3 different genomic regions: major capsid protein (MCP), DNA polymerase and NF-H1. The partial DNA polymerase gene was sequenced from all analysed viruses. The complete sequence of the MCP and a fragment of the NF-H1 gene were obtained from BIV, ECV, EHNV, ESV, FV3, PPIV, REV 282/I02 and SERV. With the exception of GV6, DFV and SGIV, the sequence analyses showed only a few variations within the analysed viruses. The sequence data suggest that PPIV, REV 282/I02 and SERV are new members of the genus Ranavirus. The methods developed in this study provide tools to differentiate between closely related ranaviruses of different host and geographical origin.
Asunto(s)
Proteínas de la Cápside/genética , ADN Polimerasa Dirigida por ADN/genética , Genes Virales/genética , Proteínas de Neurofilamentos/genética , Filogenia , Ranavirus/clasificación , Datos de Secuencia Molecular , Ranavirus/enzimología , Ranavirus/genética , Especificidad de la EspecieRESUMEN
Cytosine DNA methyltransferases (MTases) first recognize specific nucleotide sequences and then transfer a methyl group from S-adenosylmethionine to cytosine. This division of function is reflected in five highly conserved motifs shared by cytosine MTases. The region containing the first four motifs is responsible for the catalytic function whereas the region containing the fifth motif V provides specificity of binding to DNA. In at least one case, two separate proteins, one containing the first four motifs and the second containing the last motif combine to provide full functional activity. In the frog virus 3 (FV3) genome we have identified an open reading frame (ORF) whose deduced amino acid (aa) sequence contains motifs characteristic of prokaryotic as well as eukaryotic MTases. The ORF consists of 642 bp which codes for a protein of 214 aa with a predicted molecular mass of 24.8 kDa. This ORF contains the first four highly conserved motifs of cytosine MTases but the fifth motif, responsible for DNA binding specificity, is missing. Presumably, FV3 MTase is composed of two subunits. Northern blot analysis showed that the putative MTase ORF is transcribed into two transcripts belonging to the delayed-early class of FV3 messages. These two transcripts appear to be initiated at two different start sites but terminate in the same 3' region of the gene. The transcription start sites are not preceded by any known promoter sequences, but two regions of hyphenated dyad symmetry are present at the 3' end of the message. A protein with a molecular mass of approximately 28 kDa was synthesized by a rabbit reticulocyte lysate programmed with capped runoff transcripts from the cloned gene, suggesting that the ORF can be transcribed into a message coding for a viral protein. Overall, our results suggest that we have identified a gene for a subunit of MTase in the FV3 genome.
Asunto(s)
ADN-Citosina Metilasas/genética , Genes Virales/genética , Ranavirus/genética , Secuencia de Aminoácidos , Secuencia de Bases , Sistema Libre de Células , Células Cultivadas , Secuencia Conservada/genética , ADN-Citosina Metilasas/biosíntesis , ADN-Citosina Metilasas/química , Datos de Secuencia Molecular , Peso Molecular , Sistemas de Lectura Abierta/genética , ARN Mensajero/biosíntesis , ARN Viral/biosíntesis , Ranavirus/enzimología , Proteínas Recombinantes de Fusión/biosíntesis , Proteínas Recombinantes de Fusión/química , Mapeo Restrictivo , Alineación de Secuencia , Transcripción GenéticaRESUMEN
The iridovirus frog virus 3 (FV3) can replicate in culture in fat head minnow (FHM) fish cells or in BHK-21 hamster cells. Viral DNA replication commences about 3 h after infection of FHM cells with FV3. Between 3 and 6 h postinfection (p.i.), a portion of the intranuclear FV3 DNA is partly unmethylated. At later times, p.i., all of the viral DNA in the nuclear and cytoplasmic compartments is methylated at the 5'-CCGG-3' sequences. Cytoplasmic FV3 DNA has not been found unmethylated. We have cloned viral DNA fragments from methylated virion DNA. By using the genomic sequencing technique, it has been demonstrated for segments of the FV3 DNA replicated both in FHM fish and BHK21 hamster cells that in a stretch encompassing a total of 350 bp, all of the analyzed 5'-CG-3' dinucleotides are methylated. The modified nucleotide 5-methyldeoxycytidine is present exclusively in the 5'-CG-3' dinucleotide combination. In the cloned FV3 DNA fragment p21A, an open reading frame has been located. The 5' region of this presumptive viral gene is also methylated in all 5'-CG-3' positions. DNA methyltransferase activity has been detected in the nuclei of FV3-infected FHM cells at 4, 11, and 20 h p.i. In the cytoplasmic fraction, comparable activity has not been observed. These data are consistent with the interpretation that FV3 DNA is newly synthesized and de novo methylated in the nuclei of infected FHM cells and subsequently exported into the cytoplasm for viral assembly.