RESUMEN
The present study was performed to examine the histopathology, cytology, bacteriology and expression pattern of a targeted set of genes of cytokines in the oviduct of cows with inflammation (Experiment 1). In addition, the effects of oviductal fluid from cows with salpingitis on the oocyte maturation and fertilization in vitro were examined (Experiment 2). The most frequent bacterial co-infection was Escherichia coli and Fusobacterium necrophorum, which was always associated with severe histopathologic salpingitis. Out of 15 cows with histologically healthy uterus, only one cow (6.7%) displayed the histologic signs of mild salpingitis, whereas from 50 cows with endometritis, 48 cows (96%) showed histologically different grades of salpingitis. The mRNA expression of IL1ß, CD14, IL8 and CASP3 was significantly different among all groups of salpingitis (P < 0.05) with the highest level of mRNA expression in the sever grade of salpingitis. Results of experiment 2 showed a significant decline in the oocytes with peripheral free mitochondria and fertilization rate in the salpingitis group than the no- salpingitis group (P < 0.05). In conclusion, our results showed that histologically detected salpingitis is in most cases associated with histologic and cytologic endometritis. The pattern of the gene expression of chemokines and cytokines was altered in association with different grades of salpingitis. Further, we observed a decline in the peripherally located mitochondria and lower fertilization rate in oocytes following addition of oviductal fluid collected from the cows with sapingitis to the maturation media.
Asunto(s)
Bacteriología , Enfermedades de los Bovinos , Endometritis , Salpingitis , Animales , Bovinos , Enfermedades de los Bovinos/diagnóstico , Enfermedades de los Bovinos/genética , Citocinas/genética , Endometritis/veterinaria , Femenino , Oocitos , ARN Mensajero , Salpingitis/genética , Salpingitis/patología , Salpingitis/veterinaria , TranscriptomaRESUMEN
BACKGROUND: Tubal pregnancy is recognized as one of the most common ectopic pregnancy types. Salpingitis may result in tubal pregnancy by causing fallopian tube occlusion and hydrosalpinx. B cell activation factor (BAFF) is a proinflammatory cytokine that helps regulate both innate and adaptive immune responses. Our previous study firstly showed that BAFF immunostaining appeared on the cellular membrane and in the cytoplasm of tubal epithelial cells, and both BAFF protein and mRNA in human inflamed fallopian tubes had higher expression levels than those in normal fallopian tubes. This study aimed to elucidate the association between the expression of BAFF gene and the inflammation in the human fallopian tube leading to tubal pregnancy. METHODS: We examined 70 patients undergoing salpingectomy for salpingitis (n = 35) and tubal pregnancy (n = 35). Twenty patients with benign uterine diseases undergoing complete hysterectomy and salpingectomy were recruited into control group. BAFF mRNA and protein in tissue samples were detected by qPCR and Western blotting methods. Furthermore, serum levels of BAFF, tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 were measured using ELISA kits. RESULTS: We found statistically significantly elevated expressions of BAFF mRNA or protein in whole tissue samples, and serum levels of BAFF, TNF-α and IL-6 in whole blood samples from patients with salpingitis and tubal pregnancy, in comparison to the control group. CONCLUSION: Based on the results, high expression of BAFF gene might induce inflammation in the human fallopian tube, suggesting its possible role in the tubal pregnancy process.
Asunto(s)
Factor Activador de Células B/genética , Factor Activador de Células B/metabolismo , Embarazo Tubario/metabolismo , Salpingitis/genética , Salpingitis/metabolismo , Adulto , Factor Activador de Células B/sangre , Estudios de Casos y Controles , Trompas Uterinas , Femenino , Expresión Génica , Humanos , Interleucina-6/sangre , Embarazo , Embarazo Tubario/sangre , Embarazo Tubario/etiología , ARN Mensajero/metabolismo , Salpingitis/complicaciones , Factor de Necrosis Tumoral alfa/sangreRESUMEN
Women suffered with inflammatory gynecologic diseases, such as endometriosis (EMs) and acute salpingitis (AS) often complained of sub- or infertility, even in those women without obvious macroscopic anatomical pelvic abnormalities also have unexplained infertility. Generally, besides the well-known impairment of classically described oviduct cells caused by inflammatory diseases, such as the ciliated cells, fibroblasts and myofibroblasts, the involvement of the newly identified telocytes (TCs) in disease-affected oviduct tissues and potential pathophysiological roles in fertility problems remain unknown. In this chapter, TCs was investigated in rat model of EMs- and AS-affected oviduct tissues. Results showed inflammation and ischaemia-induced extensive ultrastructural damages of TCs both in cellular body and prolongations, with obvious TCs loss and interstitial fibrotic remodelling. Such in vivo pathological alterations might contribute to structural and functional abnormalities of oviduct tissue and potentially engaged in sub- or infertility. And especially, TCs connected to various activated immunocytes in both normal and diseased tissues, thus might participate in local immunoregulation (either repression or activation) and serve a possible explanation for immune-mediated pregnancy failure. Then, in vitro cell co-culture study showed that uterine TC conditioned media (TCM) can activate mouse peritoneal macrophages and subsequently trigger its cytokine secretion, thus providepreliminary evidence that, TCs are not simply innocent bystanders, but are instead potential functional players in local immunoregulatory and immunosurveillance.
Asunto(s)
Endometriosis/complicaciones , Infertilidad Femenina/complicaciones , Embarazo Ectópico/patología , Salpingitis/complicaciones , Telocitos/patología , Adherencias Tisulares/complicaciones , Animales , Antígenos CD34/genética , Antígenos CD34/metabolismo , Biomarcadores/metabolismo , Endometriosis/genética , Endometriosis/metabolismo , Endometriosis/patología , Femenino , Expresión Génica , Humanos , Infertilidad Femenina/genética , Infertilidad Femenina/metabolismo , Infertilidad Femenina/patología , Inflamación , Ratones , Embarazo , Embarazo Ectópico/genética , Embarazo Ectópico/metabolismo , Ratas , Salpingitis/genética , Salpingitis/metabolismo , Salpingitis/patología , Telocitos/metabolismo , Adherencias Tisulares/genética , Adherencias Tisulares/metabolismo , Adherencias Tisulares/patología , Útero/metabolismo , Útero/patología , Vimentina/genética , Vimentina/metabolismoAsunto(s)
Endometriosis/genética , Enfermedades de las Trompas Uterinas/genética , Proteínas Proto-Oncogénicas p21(ras)/genética , Adulto , Endometriosis/diagnóstico por imagen , Endometriosis/patología , Enfermedades de las Trompas Uterinas/diagnóstico por imagen , Enfermedades de las Trompas Uterinas/patología , Femenino , Humanos , Vasos Linfáticos/diagnóstico por imagen , Vasos Linfáticos/patología , Salpingitis/diagnóstico por imagen , Salpingitis/genética , Salpingitis/patología , Tomografía Computarizada por Rayos XRESUMEN
BACKGROUND: Hydrosalpinx are associated with infertility, due to reduced rates of implantation and increased abortion rates. The aims of this study were to investigate the expression of cystic fibrosis transmembrane conductance regulator (CFTR), nuclear factor kappa B (NF KappaB) and mucin-1 (MUC-1), and analyze the correlation between the expression of CFTR and NF KappaB or MUC1, in the endometrium of infertile women with and without hydrosalpinx. METHODS: Thirty-one infertile women with laparoscopy-confirmed unilateral or bilateral hydrosalpinx and 20 infertile women without hydrosalpinx or pelvic inflammatory disease (control group) were recruited. Endometrial biopsy samples were collected and the expression of CFTR, NF KappaB and MUC1 were analyzed using immunohistochemistry and quantitative real-time PCR. RESULTS: CFTR, NF KappaB and MUC1 mRNA and protein expression tended to increase in the secretory phase compared to the proliferative phase in both groups; however, these differences were not significantly different. The endometrium of infertile patients with hydrosalpinx had significantly higher NF KappaB mRNA and protein expression, and significantly lower CFTR and MUC1 mRNA and protein expression, compared to control infertile patients. A positive correlation was observed between CFTR and MUC1 mRNA expression (r = 0.65, P < 0.05); a negative correlation was observed between CFTR mRNA and NF KappaB mRNA expression (r = -0.59, P < 0.05). CONCLUSIONS: Increased NF KappaB expression and decreased CFTR and MUC1 expression in the endometrium of infertile patients with hydrosalpinx reinforce the involvement of a molecular mechanism in the regulation of endometrial receptivity.
Asunto(s)
Regulador de Conductancia de Transmembrana de Fibrosis Quística/biosíntesis , Regulación hacia Abajo , Endometrio/metabolismo , Infertilidad Femenina/metabolismo , Mucina-1/biosíntesis , FN-kappa B/biosíntesis , Salpingitis/metabolismo , Regulación hacia Arriba , Adulto , Regulador de Conductancia de Transmembrana de Fibrosis Quística/genética , Regulación hacia Abajo/genética , Endometrio/patología , Femenino , Humanos , Infertilidad Femenina/genética , Infertilidad Femenina/patología , Mucina-1/genética , Mucina-1/metabolismo , FN-kappa B/genética , Salpingitis/diagnóstico , Salpingitis/genética , Regulación hacia Arriba/genética , Adulto JovenRESUMEN
BACKGROUND: In women, Chlamydia (C.) trachomatis upper genital tract infection can cause distal tubal damage and occlusion, increasing the risk of tubal factor subfertility and ectopic pregnancy. Variations, like single nucleotide polymorphisms (SNPs), in immunologically important host genes are assumed to play a role in the course and outcome of a C. trachomatis infection. We studied whether genetic traits (carrying multiple SNPs in different genes) in the bacterial sensing system are associated with an aberrant immune response and subsequently with tubal pathology following a C. trachomatis infection. The genes studied all encode for pattern recognition receptors (PRRs) involved in sensing bacterial components. METHODS: Of 227 subfertile women, serum was available for C. trachomatis IgG antibody testing and genotyping (common versus rare allele) of the PRR genes TLR9, TLR4, CD14 and CARD15/NOD2. In all women, a laparoscopy was performed to assess the grade of tubal pathology. Tubal pathology was defined as extensive peri-adnexal adhesions and/or distal occlusion of at least one tube. RESULTS: Following a C. trachomatis infection (i.e. C. trachomatis IgG positive), subfertile women carrying two or more SNPs in C. trachomatis PRR genes were at increased risk of tubal pathology compared to women carrying less than two SNPs (73% vs 33% risk). The differences were not statistically significant (P = 0.15), but a trend was observed. CONCLUSION: Carrying multiple SNPs in C. trachomatis PRR genes tends to result in an aberrant immune response and a higher risk of tubal pathology following a C. trachomatis infection. Larger studies are needed to confirm our preliminary findings.
Asunto(s)
Infecciones por Chlamydia/genética , Infecciones por Chlamydia/microbiología , Chlamydia trachomatis/fisiología , Trompas Uterinas/patología , Infertilidad Femenina/genética , Infertilidad Femenina/microbiología , Anticuerpos Antibacterianos/aislamiento & purificación , Infecciones por Chlamydia/patología , Chlamydia trachomatis/patogenicidad , Trompas Uterinas/microbiología , Femenino , Predisposición Genética a la Enfermedad , Humanos , Inmunoglobulina G/aislamiento & purificación , Infertilidad Femenina/patología , Péptidos y Proteínas de Señalización Intracelular/genética , Receptores de Lipopolisacáridos/genética , Proteína Adaptadora de Señalización NOD2 , Polimorfismo de Nucleótido Simple , Salpingitis/genética , Salpingitis/microbiología , Salpingitis/patología , Receptor Toll-Like 4/genética , Receptor Toll-Like 9/genéticaRESUMEN
Perforin (pfp)/Fas ligand (FasL) double-deficient mice have previously been shown to be infertile, lose weight and die prematurely due to tissue destruction caused by a significant inflammatory infiltrate of monocytes/macrophages and T cells. Herein we have compared disease progression in mice additionally deficient in the inflammatory mediator TNF. Unlike pfp/FasL double-deficient mice (TNF+/+ pfp-/- gld), mice lacking functional TNF, FasL and pfp (TNF-/- pfp-/- gld) were comparatively fertile, with the majority of mice not suffering severe pancreatitis or hysterosalphingitis in the first 5 months of life. The mean lifespan of TNF-/- pfp-/- gld mice was 217 +/- 79 days compared with 69 +/- 10 days for TNF+/+ pfp-/- gld mice and the majority of moribund TNF-/- pfp-/- gld mice appeared to die as a result of severe pancreatitis, suggesting that loss of TNF was not completely protective. At 8 weeks of age, characteristics associated with the gld phenotype, such as expansion of B220+ CD4- CD8- T cells, lymphadenopathy and hypergammaglobulinemia were comparable between TNF+/+ pfp-/- gld and TNF-/- pfp-/- gld mice, although the lymphoid organs of TNF+/+ pfp-/- gld mice contained greater numbers of B220+ CD4- CD8- T cells, macrophages and T cells. We conclude that TNF is necessary for the full manifestation of immune dysregulation caused by pfp/FasL-deficiency, in particular in the early and overwhelming tissue infiltration and destruction caused by inflammatory cells.
Asunto(s)
Endometritis/patología , Síndromes de Inmunodeficiencia/patología , Enfermedades Linfáticas/patología , Trastornos Linfoproliferativos/patología , Glicoproteínas de Membrana/deficiencia , Pancreatitis/patología , Salpingitis/patología , Esplenomegalia/patología , Factor de Necrosis Tumoral alfa/fisiología , Animales , Modelos Animales de Enfermedad , Progresión de la Enfermedad , Endometritis/genética , Endometritis/inmunología , Proteína Ligando Fas , Femenino , Histiocitosis de Células no Langerhans/genética , Histiocitosis de Células no Langerhans/inmunología , Síndromes de Inmunodeficiencia/genética , Infertilidad Femenina/etiología , Longevidad , Enfermedades Linfáticas/genética , Enfermedades Linfáticas/inmunología , Trastornos Linfoproliferativos/genética , Trastornos Linfoproliferativos/inmunología , Masculino , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/fisiología , Ratones , Ratones Endogámicos C57BL , Pancreatitis/genética , Pancreatitis/inmunología , Perforina , Fenotipo , Proteínas Citotóxicas Formadoras de Poros , Salpingitis/genética , Salpingitis/inmunología , Esplenomegalia/genética , Esplenomegalia/inmunología , Subgrupos de Linfocitos T/inmunología , Subgrupos de Linfocitos T/patología , Factor de Necrosis Tumoral alfa/deficiencia , Factor de Necrosis Tumoral alfa/genéticaRESUMEN
OBJECTIVE: To determine the effect of hydrosalpinx fluid on the expression of HOXA10, an essential regulator of endometrial receptivity. DESIGN: In vitro study. SETTING: Academic medical center. PATIENT(S): Patients with unilateral or bilateral hydrosalpinx. INTERVENTION(S): Hydrosalpinx fluid was aspirated from 10 patients at laparoscopy. The fluid was serially diluted in minimum essential medium. Ishikawa cells (an endometrial adenocarcinoma cell line, representative of endometrial epithelium) were incubated with this fluid at concentrations of 10% and 50% for 48 hours. Cells were also incubated in undiluted minimum essential medium (MEM) and in 10% serum as controls. After incubation, the cells were lysed in Trizol, and total RNA was extracted and analyzed by Northern blot using a 32P-labeled HOXA10 riboprobe. A 32P-labeled G3PDH probe was used as a control for loading. MAIN OUTCOME MEASURE(S): HOXA10 mRNA expression. RESULT(S): HOXA10 mRNA expression in endometrial cells decreased with increasing concentrations of hydrosalpinx fluid. Densitometric analysis of the northern blot revealed that HOXA10 mRNA expression was different from control at both concentrations (P<.007). CONCLUSION(S): HOXA10 is necessary for implantation in the murine model. HOXA10 expression is diminished by hydrosalpinx fluid. This effect on HOXA10 is a potential molecular mechanism by which implantation rates are diminished in women with hydrosalpinges.
Asunto(s)
Endometrio/fisiopatología , Enfermedades de las Trompas Uterinas/fisiopatología , Genes Homeobox , Salpingitis/fisiopatología , Northern Blotting , Líquidos Corporales/fisiología , Endometrio/fisiología , Enfermedades de las Trompas Uterinas/genética , Femenino , Humanos , Infertilidad Femenina/etiología , Salpingitis/genéticaRESUMEN
We report that perforin/Fas-ligand double-deficient mice die early of severe pancreatitis. Female mice, in addition, are infertile and suffer from hysterosalpingitis. Tissue destruction is accompanied by infiltration with Mac-1 (CD11b)-positive monocytes/macrophages, Mac-1-positive T cells, and expansion of CD8+ T cells. In vivo inactivation of monocytes/macrophages by carrageenan reverses disease progression and restores fertility of female mice. Perforin/Fas-ligand double-deficient CD4+ or CD8+ CTL are unable to lyse cognate-activated macrophages, and therefore are unable to mediate negative feedback regulation by lysis of APCs, thereby preventing further T cell activation. These studies demonstrate a novel role for perforin in homeostatic regulation of the immune response.
Asunto(s)
Enfermedades Autoinmunes/patología , Activación de Macrófagos , Macrófagos/patología , Glicoproteínas de Membrana/fisiología , Pancreatitis/patología , Subgrupos de Linfocitos T/patología , Animales , Presentación de Antígeno , Enfermedades Autoinmunes/tratamiento farmacológico , Enfermedades Autoinmunes/genética , Enfermedades Autoinmunes/inmunología , Linfocitos T CD4-Positivos/inmunología , Linfocitos T CD4-Positivos/patología , Linfocitos T CD8-positivos/inmunología , Linfocitos T CD8-positivos/patología , Carragenina/uso terapéutico , Citotoxicidad Inmunológica , Endometritis/genética , Endometritis/inmunología , Endometritis/patología , Proteína Ligando Fas , Retroalimentación , Femenino , Infertilidad Femenina/tratamiento farmacológico , Infertilidad Femenina/genética , Infertilidad Femenina/inmunología , Infertilidad Femenina/patología , Activación de Linfocitos , Activación de Macrófagos/efectos de los fármacos , Antígeno de Macrófago-1/análisis , Glicoproteínas de Membrana/deficiencia , Glicoproteínas de Membrana/genética , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Neoplasias Experimentales/patología , Páncreas/patología , Pancreatitis/genética , Pancreatitis/inmunología , Perforina , Proteínas Citotóxicas Formadoras de Poros , Embarazo , Salpingitis/genética , Salpingitis/inmunología , Salpingitis/patología , Subgrupos de Linfocitos T/inmunología , Linfocitos T Citotóxicos/inmunología , Linfocitos T Citotóxicos/patología , Células Tumorales Cultivadas , Útero/patologíaRESUMEN
Groups of mice from genetically defined inbred strains were infected genitally with a pathogenic human strain of Chlamydia trachomatis and their subsequent fertility was compared. The CBA, C3H (H-2o) and C3H/He-mg (H-2k) mice were less fertile than control mice, at least up to 6 months after infection. In contrast, fertility was not impaired in BALB/c mice or in congenic BALB/K mice, which had the H-2k haplotype. Reduced fertility was paralleled by the extent of histological oviductal inflammation in mice of each strain. No salpingitis was seen 21 days after infection in the BALB strains, but lesions were apparent in CBA and C3H strains up to about 70 days after inoculation and these sometimes developed into hydrosalpinges. These results indicate that susceptibility to chlamydial salpingitis and subsequent infertility is under genetic control. This control was not simply associated with the major H-2 gene complex, as mouse strains of the same haplotype (H-2k) differed in susceptibility. The fertility of BALB/c (H-2d) and BALB/K (H-2k) strains was no different from that of controls, and congenic C3H mice of differing H-2 haplotypes (H-2k and H-2o) showed reduced fertility. Although all the infected F1 (BALB/K x C3H/He-mg) mice produced litters at the same rate as untreated controls, the litters were considerably smaller. This was due to the occurrence of unilateral pregnancies in the mice inoculated under the ovarian bursae and possibly also to early fetal death in mice inoculated directly in the uterus. These findings emphasize the importance of early diagnosis and treatment of infection of the lower genital tract of women.