RESUMEN
Vaccinia virus (VACV) is the causative agent of an emerging viral zoonosis called bovine vaccinia (BV). Several studies have documented characteristics of VACV infections in Brazil; however, the manner in which this virus is maintained in wildlife remains unknown. This work investigated the presence of viral DNA and anti-orthopoxvirus (OPXV) antibodies in samples collected from small mammals in a VACV-endemic area in Minas Gerais, Brazil, in the absence of current outbreaks. Samples did not show amplification of OPXV DNA in molecular tests. However, 5/142 serum samples demonstrated the presence of anti-OPXV neutralizing antibodies in serological tests. These data reinforce the involvement of small mammals in the natural cycle of VACV, highlighting the need for further ecological studies to better understand how this virus is maintained in nature and to develop measures to prevent BV outbreaks.
Asunto(s)
Enfermedades Transmisibles , Orthopoxvirus , Vaccinia , Animales , Bovinos , Orthopoxvirus/genética , Zoonosis , Brasil/epidemiología , Virus Vaccinia/genética , Vaccinia/epidemiología , Vaccinia/veterinaria , Enfermedades Transmisibles/epidemiología , Brotes de Enfermedades , MamíferosRESUMEN
Bovine vaccinia (BV) is an infectious disease caused by Vaccinia virus (VACV) characterized by vesicular and exanthematic lesions, mainly in cattle. Although BV has been described in some Brazilian regions in the last decades, official information regarding the current prevalence in bovine herds of Midwestern Brazil is lacking. Thus, the current study aimed to estimate the seroprevalence and risk factors associated with BV in cattle in the Distrito Federal (DF), Brazil. Sera of 312 cows of 64 herds were tested by virus-neutralizing test for VACV antibodies. Herd and animal seroprevalence were estimated to be 33.3% (CI 95%: 18.2-48.3%) and 10.6% (CI 95%: 1.0-20.2%), respectively. Seropositive cows were detected in dairy, beef, and mixed-purpose farms. The results of an epidemiological questionnaire showed that no risk factor analyzed was positively associated with seropositivity to VACV. There was no significant association between type of milking (manual/mechanic) and seropositivity to VACV; however, most seropositive cows were present in farms with high daily milk production and high number of lactating and adult cows. Our results indicate that VACV circulates in many regions of DF with considerable prevalence in dairy cows. Control measures to restrict VACV circulation and consequences of the infection may be advisable.
Asunto(s)
Enfermedades de los Bovinos , Vaccinia , Animales , Brasil/epidemiología , Bovinos , Enfermedades de los Bovinos/epidemiología , Granjas , Femenino , Lactancia , Estudios Seroepidemiológicos , Vaccinia/epidemiología , Vaccinia/veterinaria , Virus VacciniaRESUMEN
Outbreaks of a vesiculopustular disease in dairy cattle and milkers have been frequently reported in Brazil since 1999 when the vaccinia virus strain Cantagalo was first isolated in the State of Rio de Janeiro. However, the genomic diversity of the viral isolates associated with these outbreaks is not well known, particularly in the southeastern states that represent the focal point of virus spread to other regions. Here, we report the genomic sequences and an analysis of the polymorphic site profiles and genotypic diversity of four clinical isolates of vaccinia virus strain Cantagalo collected from 1999 to 2006 in southeastern Brazil.
Asunto(s)
Enfermedades de los Bovinos , Virus Vaccinia , Vaccinia , Animales , Brasil/epidemiología , Bovinos , Enfermedades de los Bovinos/epidemiología , Brotes de Enfermedades , Genómica , Filogenia , Vaccinia/epidemiología , Vaccinia/veterinaria , Virus Vaccinia/genéticaRESUMEN
A retrospective study of officially diagnosed poxvirus infections in cattle in Distrito Federal (DF), Brazil, between 2015 and 2018 was performed. All cases were investigated by the DF Official Veterinary Service. In the most cases, samples of oral, cutaneous (teats, udder) or foot lesions were submitted to molecular diagnosis by PCR. In approximately 70% of the cases, additional samples were also submitted for histopathology. Ninety-three out of 2,467 clinically examined cattle (from 385 farms) presented suggestive and/or compatible lesions with poxviruses. Fifty-two out of these 93 cases were confirmed as poxviruses: 27 vaccinia virus (VACV), 9 pseudocowpox virus (PCPV), 8 bovine papular stomatitis virus (BPSV), 5 coinfection by PCPV and BPSV and 3 unidentified parapoxvirus. The clinical cases were observed in farms with different exploration (beef, dairy or mixed) from 9 out of 30 administrative regions of DF. Gross findings consisted of papules, vesicles, ulcers, scabs and scars and varied of type, severity and affected tissue, according to the detected virus. A single human case was observed associated with a BPSV infection. Histologically, the lesions were very similar, independently of the detected poxvirus, and included mild to moderate, superficial, multifocal inflammatory infiltrate of lymphocytes, plasma cells, macrophages and/or neutrophils, with acanthosis and parakeratotic hyperkeratosis, usually associated with serous content, cellular debris and spongiosis. In the ulcerated lesions, there were focally extensive areas of necrosis with severe infiltrate of neutrophils in the adjacent connective tissue. Few to moderate amount of 4- to 8-µm eosinophilic inclusion bodies were observed in the cytoplasm of keratinocytes in 6 cases (2 of VACV, 2 of PCPV and 2 of PCPV/BPSV coinfection). Data of the current study demonstrate the wide circulation of different poxviruses in cattle from DF.
Asunto(s)
Enfermedades de los Bovinos/virología , Parapoxvirus/aislamiento & purificación , Infecciones por Poxviridae/veterinaria , Virus Vaccinia/aislamiento & purificación , Vaccinia/veterinaria , Animales , Brasil/epidemiología , Bovinos , Enfermedades de los Bovinos/epidemiología , Coinfección/veterinaria , Brotes de Enfermedades/veterinaria , Humanos , Parapoxvirus/genética , Reacción en Cadena de la Polimerasa/veterinaria , Infecciones por Poxviridae/epidemiología , Infecciones por Poxviridae/virología , Estudios Retrospectivos , Vaccinia/epidemiología , Vaccinia/virología , Virus Vaccinia/genéticaRESUMEN
The aim of this study was to evaluate the possibility of free-ranging animals/hunting dogs as sources of infection in the vaccinia virus (VACV) transmission chain. Serological, cell culture and molecular assays were conducted in 56 free-ranging animals and 22 hunting dogs. ELISA/neutralizing assays showed that two (2.5%) capybaras (Hydrochoerus hydrochaeris) had anti-OPV positive antibodies, while all samples tested negative through PCR/cell culture. After being hit by cars on roads, capybaras that exhibited neither clinical signs nor any association with bovine outbreaks had neutralizing antibodies against the Orthopoxvirus, as detected through plaque-reduction neutralizing tests and ELISA. Evidence exists regarding peridomestic capybaras acting as a source of the virus and serving as a link between wild and urban environments, thus contributing to viral maintenance.
Asunto(s)
Reservorios de Enfermedades/veterinaria , Enfermedades de los Perros/transmisión , Enfermedades de los Roedores/epidemiología , Roedores/virología , Virus Vaccinia/inmunología , Vaccinia/veterinaria , Animales , Animales Salvajes , Anticuerpos Neutralizantes , Brasil/epidemiología , Bovinos , Reservorios de Enfermedades/virología , Enfermedades de los Perros/epidemiología , Enfermedades de los Perros/virología , Perros , Pruebas de Neutralización/veterinaria , Enfermedades de los Roedores/virología , Vaccinia/epidemiología , Vaccinia/transmisión , Vaccinia/virología , Virus Vaccinia/aislamiento & purificación , ZoonosisRESUMEN
Outbreaks of a vesiculopustular disease in dairy cattle and milkers have been frequently reported in Brazil since 1999 when the vaccinia virus strain Cantagalo was first isolated in the State of Rio de Janeiro. However, the genomic diversity of the viral isolates associated with these outbreaks is not well known, particularly in the southeastern states that represent the focal point of virus spread to other regions. Here, we report the genomic sequences and an analysis of the polymorphic site profiles and genotypic diversity of four clinical isolates of vaccinia virus strain Cantagalo collected from 1999 to 2006 in southeastern Brazil.
Asunto(s)
Animales , Bovinos , Vaccinia/veterinaria , Vaccinia/epidemiología , Virus Vaccinia/genética , Enfermedades de los Bovinos/epidemiología , Filogenia , Brasil/epidemiología , Brotes de Enfermedades , GenómicaRESUMEN
To determine their potential role as a source of human infection, we tested domestic dogs (urban) and wild coatis (wild) in Brazil for vaccinia virus. Our findings of positive neutralizing antibodies and quantitative PCR results for 35/184 dogs and 13/90 coatis highlight a potential public health risk.
Asunto(s)
Enfermedades de los Animales/epidemiología , Enfermedades de los Animales/virología , Vaccinia/veterinaria , Enfermedades de los Animales/diagnóstico , Animales , Anticuerpos Neutralizantes , Anticuerpos Antivirales , Brasil/epidemiología , ADN Viral , Brotes de Enfermedades , Perros , Pruebas de Neutralización , Reacción en Cadena de la Polimerasa , Procyonidae , Virus VacciniaRESUMEN
Bovine vaccinia (BV), caused by Vaccinia virus (VACV), is a zoonosis characterized by exanthematous lesions in the teats of dairy cows and the hands of milkers and is an important public health issue. Severe VACV-induced lesions in the teats and udder of cows and buffaloes could lead to mastitis and other secondary infections, thereby reducing productivity and resulting in economic losses to the dairy industry. In Brazil, BV re-emerged in the late 1990s and is now endemic in most of the Brazilian territory. In the last 15 years, much effort has been made to know more about this disease and its epidemiology, etiologic agents, and interactions with the host and the environment. In this review, we describe the known dynamics of VACV infection in cattle and the viral shedding routes, as well as the relevance of BV for animal and public health.
Asunto(s)
Enfermedades de los Bovinos/virología , Virus Vaccinia/fisiología , Vaccinia/veterinaria , Zoonosis/virología , Animales , Brasil/epidemiología , Bovinos , Enfermedades de los Bovinos/transmisión , Humanos , Salud Pública , Vaccinia/transmisión , Vaccinia/virología , Virus Vaccinia/aislamiento & purificación , Esparcimiento de Virus , Zoonosis/transmisiónRESUMEN
The origin of Vaccinia virus (VACV) outbreaks in Brazil remains unknown, but since the isolation of VACV in Mus musculus mice during a zoonotic outbreak affecting cattle and milkers, peridomestic rodents have been suggested to be a link between cows and wild animals. Considering that experimentally infected mice eliminate viral particles in their feces, we investigated the presence of VACV in the feces and urine of wild rodents that were captured in the forest areas surrounding milking farms in the central west region of São Paulo State. For the first time, this work reports the detection of VACV by PCR in the feces of naturally infected Oligoryzomys flavescens, Oligoryzomys nigripes, and Sooretamys angouya, and in the urine of Oligorizomys flavescens, which raises important questions about the spread of VACV by rodent feces and its potential to induce clinical infections in cows.
Asunto(s)
Enfermedades de los Animales/epidemiología , Enfermedades de los Animales/virología , Animales Salvajes , Roedores , Virus Vaccinia , Vaccinia/veterinaria , Esparcimiento de Virus , Enfermedades de los Animales/transmisión , Animales , Brasil/epidemiología , Biología Computacional/métodos , ADN Viral , Brotes de Enfermedades/veterinaria , Granjas , Heces/virología , Bosques , Geografía Médica , Filogenia , Reacción en Cadena de la Polimerasa , Análisis de Secuencia de ADN , Virus Vaccinia/genética , Virus Vaccinia/aislamiento & purificaciónRESUMEN
Differentiation of naive CD4+ T-cells into functionally distinct T helper (Th) subsets is critical to immunity against pathogen infection. Little is known about the role of signals emanating from the nuclear envelope for T-cell differentiation. The nuclear envelope protein lamin A/C is induced in naive CD4+ T-cells upon antigen recognition and acts as a link between the nucleus and the plasma membrane during T-cell activation. Here we demonstrate that the absence of lamin A/C in naive T-cell reduces Th1 differentiation without affecting Th2 differentiation in vitro and in vivo. Moreover, Rag1 -/- mice reconstituted with Lmna -/- CD4+CD25 - T-cells and infected with vaccinia virus show weaker Th1 responses and viral removal than mice reconstituted with wild-type T-cells. Th1 responses and pathogen clearance upon Leishmania major infection were similarly diminished in mice lacking lamin A/C in the complete immune system or selectively in T-cells. Lamin A/C mediates Th1 polarization by a mechanism involving T-bet and IFNγ production. Our results reveal a novel role for lamin A/C as key regulator of Th1 differentiation in response to viral and intracellular parasite infections.
Asunto(s)
Lamina Tipo A/genética , Leishmaniasis Cutánea/patología , Células TH1/metabolismo , Vaccinia/patología , Animales , Linfocitos T CD4-Positivos/citología , Linfocitos T CD4-Positivos/metabolismo , Diferenciación Celular , Susceptibilidad a Enfermedades , Sistema Inmunológico/metabolismo , Interferón gamma/metabolismo , Interleucina-4/metabolismo , Lamina Tipo A/deficiencia , Leishmania major/patogenicidad , Leishmaniasis Cutánea/inmunología , Leishmaniasis Cutánea/veterinaria , Activación de Linfocitos , Ratones , Ratones Endogámicos BALB C , Proteínas de Dominio T Box/metabolismo , Células TH1/citología , Células TH1/inmunología , Vaccinia/inmunología , Vaccinia/veterinaria , Virus Vaccinia/patogenicidadRESUMEN
Vaccinia virus (VACV) is the agent of bovine vaccinia (BV), an emerging zoonosis that causes exanthematic lesions on the teats of dairy cows and on the hands of milkers. The virus has been detected in the milk of naturally infected cows. The objective of this study was to investigate and quantify VACV DNA as well as the presence of infectious virus particles in samples of cheese curd, cheese whey and pasteurized milk produced using milk from cows experimentally inoculated with VACV-GP2, a Brazilian isolate of VACV (VACV-BR). VACV DNA was detected in samples of cheese and pasteurized milk at different time points, even after the resolution of the typical lesions caused by VACV, which occurred after 22 days post-infection (dpi), on average. Moreover, it was possible to detect infectious viral particles in cheese samples on alternate days until 27 dpi. The presence of both VACV DNA and infectious viral particles in cheese samples throughout the clinical course of BV and even after the disappearance of the typical clinical signs of disease draws attention to the risk associated with consumption of the cheese. Furthermore, VACV-contaminated milk and cheese may represent an occupational risk to cheesemakers who often manipulate milk and cheese curd without wearing gloves.
Asunto(s)
Enfermedades de los Bovinos/virología , Productos Lácteos/virología , Enfermedades Transmitidas por los Alimentos/virología , Leche/virología , Virus Vaccinia/aislamiento & purificación , Vaccinia/veterinaria , Animales , Bovinos , Queso/virología , ADN Viral/análisis , Femenino , Reacción en Cadena de la Polimerasa/veterinaria , Salud Pública , Vaccinia/virología , Virus Vaccinia/genética , ZoonosisRESUMEN
Bovine vaccinia (BV) is a zoonosis caused by Vaccinia virus (VACV) that mainly affects lactating cows and dairy farm milkers. The epidemiological role(s) of other cattle categories such as dry cows, bulls, and heifers in BV remains unclear. This study was performed to investigate VACV in affected dairy cattle herds and perifocal farms during an outbreak in Brazil. Crusts from lesions of cows' teats were collected from all farms with BV outbreaks. Milk, feces, blood, and serum were collected from symptomatic and asymptomatic lactating cows. Blood and serum were also sampled from other cattle categories (calves, heifers, dry cows, and bulls). The samples were tested for VACV by PCR, and to confirm VACV viability, VACV-positive samples were inoculated in BSC-40 cells and stained using immunoperoxidase. Neutralizing antibodies were investigated using plaque reduction neutralization test. Viral DNA was detected in milk, blood, and feces samples of symptomatic and asymptomatic dairy cows and in blood samples from other cattle categories on farms with and without confirmed BV outbreak. In affected farms, viable virus was identified in feces and milk samples from lactating cows and in blood samples from asymptomatic dry cows. Viable VACV was also identified in feces from lactating cows and one bull's blood sample from perifocal farms. Neutralizing antibodies were detected in 81.6% of the herds affected by BV and in 53.8% of the herds on perifocal farms. The presented data indicate a potential source of viral dissemination, which contributes to the persistence and spread of VACV in the environment.
Asunto(s)
Infecciones Asintomáticas/epidemiología , Enfermedades de los Bovinos/epidemiología , Virus Vaccinia/aislamiento & purificación , Vaccinia/veterinaria , Animales , Sangre/virología , Brasil/epidemiología , Bovinos , Enfermedades de los Bovinos/virología , ADN Viral/análisis , Heces/virología , Femenino , Masculino , Leche/virología , Factores de Riesgo , Vaccinia/epidemiología , Vaccinia/virología , Zoonosis/epidemiología , Zoonosis/virologíaRESUMEN
Orthopoxviruses (OPV) are emerging viruses with great importance in human and veterinary medicine, such as Vaccinia virus (VACV), which causes outbreaks of bovine vaccinia (BV) in South America. The clinical aspects of BV are similar to other vesicular infections, complicating the clinical diagnosis. This cross-sectional study evaluated the knowledge of Healthcare Professionals about BV and revealed their unpreparedness about BV in a VACV hyper-endemic area in Brazil, highlighting the public health issues associated with VACV infections. This study presents an opportunity to discuss the importance of vaccination for healthcare professionals who work in areas of VACV circulation and brings an educational measure on VACV infections for health professionals around the world.
Asunto(s)
Enfermedades Endémicas , Conocimientos, Actitudes y Práctica en Salud , Personal de Salud , Vaccinia , Adulto , Animales , Brasil/epidemiología , Bovinos , Enfermedades de los Bovinos/epidemiología , Enfermedades de los Bovinos/virología , Estudios Transversales , Enfermedades Endémicas/veterinaria , Femenino , Humanos , Masculino , Filogenia , Pruebas Serológicas , Vacunación , Vaccinia/diagnóstico , Vaccinia/epidemiología , Vaccinia/veterinaria , Virus Vaccinia/clasificación , Virus Vaccinia/genética , Virus Vaccinia/inmunología , Virus Vaccinia/aislamiento & purificación , ZoonosisRESUMEN
Vaccinia virus (VACV) is a zoonotic agent that causes a disease called bovine vaccinia, which is detected mainly in milking cattle and humans in close contact with these animals. Even though many aspects of VACV infection have been described, much is still unknown about its circulation in the environment and its natural hosts/reservoirs. To investigate the presence of Orthopoxvirus antibodies or VACV DNA, we captured small rodents and marsupials in 3 areas of Minas Gerais state, Brazil, and tested their samples in a laboratory. A total of 336 animals were tested; positivity ranged from 18.1% to 25.5% in the 3 studied regions located in different biomes, including the Atlantic Forest and the Cerrado. Analysis of nucleotide sequences indicated co-circulation of VACV groups I and II. Our findings reinforce the possible role played by rodents and marsupials in VACV maintenance and its transmission chain.
Asunto(s)
Anticuerpos Antivirales/sangre , Enfermedades de los Bovinos/epidemiología , ADN Viral/sangre , Brotes de Enfermedades , Marsupiales/virología , Roedores/virología , Vaccinia/epidemiología , Animales , Brasil/epidemiología , Bovinos , Enfermedades de los Bovinos/sangre , Enfermedades de los Bovinos/transmisión , Reservorios de Enfermedades/virología , Incidencia , Tipificación Molecular , Vaccinia/sangre , Vaccinia/transmisión , Vaccinia/veterinaria , Virus Vaccinia/clasificación , Virus Vaccinia/genética , Virus Vaccinia/patogenicidadRESUMEN
Vaccinia virus (VACV) circulates in Brazil and other South America countries and is responsible for a zoonotic disease that usually affects dairy cattle and humans, causing economic losses and impacting animal and human health. Furthermore, it has been detected in wild areas in the Brazilian Amazon. To better understand the natural history of VACV, we investigated its circulation in wildlife from French Guiana, a remote region in the Northern Amazon forest. ELISA and plaque reduction neutralization tests were performed to detect anti-orthopoxvirus antibodies. Real-time and standard PCR targeting C11R, A56R and A26L were applied to detect VACV DNA in serum, saliva and tissue samples. No evidence of VACV infection was found in any of the samples tested. These findings provide additional information on the VACV epidemiological puzzle. The virus could nevertheless be circulating at low levels that were not detected in areas where no humans or cattle are present.
Asunto(s)
Animales Salvajes/virología , Virus Vaccinia/aislamiento & purificación , Vaccinia/veterinaria , Animales , Anticuerpos Antivirales/sangre , Ensayo de Inmunoadsorción Enzimática , Bosques , Guyana Francesa/epidemiología , Mamíferos/virología , Saliva/virología , Vaccinia/epidemiología , Vaccinia/virología , Virus Vaccinia/inmunología , Ensayo de Placa ViralRESUMEN
We investigated possible vaccinia virus (VACV) in urban house cats in Brazil. Serum samples from 6 cats were positive for VACV by PCR, indicating likely VACV circulation among house cats in urban areas of Brazil. This finding highlights the importance of epidemiologic surveillance to avoid outbreaks among urban human populations.
Asunto(s)
Enfermedades de los Gatos/epidemiología , Enfermedades de los Gatos/virología , Salud Urbana , Virus Vaccinia , Vaccinia/veterinaria , Animales , Animales Domésticos , Anticuerpos Neutralizantes/inmunología , Anticuerpos Antivirales/inmunología , Brasil/epidemiología , Enfermedades de los Gatos/inmunología , Gatos , Filogenia , Vigilancia en Salud Pública , Virus Vaccinia/clasificación , Virus Vaccinia/genética , Virus Vaccinia/inmunología , Proteínas de la Matriz Viral/genética , Proteínas de la Matriz Viral/inmunologíaRESUMEN
REASONS FOR PERFORMING STUDY: In August 2014, an outbreak of oral exanthematous disease in equids was reported in Brazil, affecting 11 donkeys and 3 mules. OBJECTIVES: To investigate if Vaccinia virus (VACV) was the aetiological agent in this outbreak. STUDY DESIGN: Investigation of clinical cases using serological, molecular and phylogenetic approaches. METHODS: To analyse the presence of neutralising antibodies against VACV, samples were submitted in triplicate to a plaque-reduction neutralisation test (PRNT50% ). On the basis of previous studies which detected VACV DNA in sera, we submitted extracted DNA samples to different polymerase chain reaction (PCR) platforms targeting Orthopoxvirus (OPV) genes (C11R, A56R and A26L). The PCR products were directly sequenced in both orientations using specific primers and capillary electrophoresis. The alignment and phylogenetic analysis of the A26L and A56R nucleotide sequences (maximum likelihood) were prepared with the obtained nucleotide fragments. RESULTS: Serological and molecular data suggested VACV as the aetiological agent. The neutralising antibodies against OPV were detected in 5 (55.5%) of the equids, with titres ≥40 neutralising u/ml. Based on the results obtained from all PCR platforms, all samples were positive for OPV: 9 (100%) for A56R, 4 (44.4%) for C11R and 3 (33.3%) for A26L. The alignment of the nucleotide sequences of the A26L and A56R fragments revealed that the samples were highly similar to the homologous genes from other Brazilian VACV Group 1 isolates (98.8% identity on average). Furthermore, both the A26L and A56R sequences showed signature deletions also present in the sequences of Group 1 VACV isolates from Brazil. CONCLUSIONS: Our data raises questions about the role of equids in the chain of VACV epidemiology. The surveillance of equids in VACV-affected areas worldwide is relevant.
Asunto(s)
Brotes de Enfermedades/veterinaria , Equidae , Exantema/veterinaria , Enfermedades de la Boca/veterinaria , Virus Vaccinia/aislamiento & purificación , Vaccinia/veterinaria , Animales , Brasil/epidemiología , Exantema/patología , Exantema/virología , Enfermedades de la Boca/epidemiología , Enfermedades de la Boca/patología , Enfermedades de la Boca/virología , Vaccinia/diagnóstico , Vaccinia/epidemiología , Vaccinia/patologíaRESUMEN
Vaccinia virus (VACV) is the etiological agent of bovine vaccinia (BV), an emerging zoonosis that has been associated with economic losses and social effects. Despite increasing reports of BV outbreaks in Brazil, little is known about the biological interactions of Brazilian VACV (VACV-BR) isolates during coinfections; furthermore, there are no tools for the diagnosis of these coinfections. In this study, a tool to co-detect two variants of VACV was developed to provide new information regarding the pathogenesis, virulence profile, and viral spread during coinfection with VACV-BR isolates. To test the quantitative polymerase chain reactions (qPCR) tool, groups of BALB/c mice were intranasally monoinfected with Pelotas virus 1-Group II (PV1-GII) and Pelotas virus 2-Group I (PV2-GI), or were coinfected with PV1-GII and PV2-GI. Clinical signs of the mice were evaluated and the viral load in lung and spleen were detected using simultaneous polymerase chain reactions (PCR) targeting the A56R (hemagglutinin) gene of VACV. The results showed that qPCR for the quantification of viral load in coinfection was efficient and highly sensitive. Coinfected mice presented more severe disease and a higher frequency of VACV detection in lung and spleen, when compared to monoinfected groups. This study is the first description of PV1 and PV2 pathogenicity during coinfection in mice, and provides a new method to detect VACV-BR coinfections.
Asunto(s)
Enfermedades de los Bovinos/diagnóstico , Coinfección/veterinaria , Reacción en Cadena de la Polimerasa , Virus Vaccinia/fisiología , Vaccinia/veterinaria , Animales , Brasil , Bovinos , Enfermedades de los Bovinos/virología , Coinfección/diagnóstico , Coinfección/virología , Hemaglutininas Virales/genética , Masculino , Ratones , Ratones Endogámicos BALB C , Vaccinia/diagnóstico , Vaccinia/virología , Virus Vaccinia/clasificación , Virus Vaccinia/genética , Virus Vaccinia/patogenicidad , Carga Viral , VirulenciaRESUMEN
We detected orthopoxvirus in 28 of 125 serum samples collected during 2009 from cattle in Uruguay. Two samples were PCR-positive for vaccinia virus and had sequences similar to those for vaccinia virus associated with outbreaks in Brazil. Autochthonous circulation of vaccinia virus in Uruguay and other South American countries cannot be ruled out.
Asunto(s)
Enfermedades de los Bovinos/epidemiología , Enfermedades de los Bovinos/virología , Virus Vaccinia/genética , Vaccinia/veterinaria , Animales , Bovinos , Brotes de Enfermedades , Genes Virales , Geografía Médica , ARN Viral , América del Sur/epidemiología , Uruguay/epidemiología , Virus Vaccinia/clasificación , Virus Vaccinia/aislamiento & purificación , ZoonosisRESUMEN
Natural infections of Vaccinia virus (VACV)-the prototype species of the Orthopoxvirus genus, from the family Poxviridae and subfamily Chordopoxvirinae-cause an occupational emergent zoonotic disease that is primarily associated with the handling of infected dairy cattle. In humans, VACV infection is characterized by skin lesions, primarily on the hands, and accompanied by systemic symptoms such as fever, myalgia, headache, and lymphadenopathy. The diagnosis of VACV is usually performed according to the methods described for other orthopoxviruses. This unit describes the methods utilized to obtain clinical samples, the serological and molecular techniques used for diagnosis, and the isolation methods and techniques used for molecular and biological characterization of the viruses. © 2016 by John Wiley & Sons, Inc.