RESUMEN
A novel mycovirus named Fusarium oxysporum alternavirus 1(FoAV1) was identified as infecting Fusarium oxysporum strain BH19, which was isolated from a fusarium wilt diseased stem of Lilium brownii. The genome of FoAV1 contains four double-stranded RNA (dsRNA) segments (dsRNA1, dsRNA 2, dsRNA 3 and dsRNA 4, with lengths of 3.3, 2.6, 2.3 and 1.8 kbp, respectively). Additionally, dsRNA1 encodes RNA-dependent RNA polymerase (RdRp), and dsRNA2- dsRNA3- and dsRNA4-encoded hypothetical proteins (ORF2, ORF3 and ORF4), respectively. A homology BLAST search, along with multiple alignments based on RdRp, ORF2 and ORF3 sequences, identified FoAV1 as a novel member of the proposed family "Alternaviridae". Evolutionary relation analyses indicated that FoAV1 may be related to alternaviruses, thus dividing the family "Alternaviridae" members into four clades. In addition, we determined that dsRNA4 was dispensable for replication and may be a satellite-like RNA of FoAV1-and could perhaps play a role in the evolution of alternaviruses. Our results provided evidence for potential genera establishment within the proposed family "Alternaviridae". Additionally, FoAV1 exhibited biological control of Fusarium wilt. Our results also laid the foundations for the further study of mycoviruses within the family "Alternaviridae", and provide a potential agent for the biocontrol of diseases caused by F. oxysporum.
Asunto(s)
Virus Fúngicos/genética , Virus Fúngicos/aislamiento & purificación , Fusarium/virología , Virus no Clasificados/genética , Virus no Clasificados/aislamiento & purificación , Virus Fúngicos/clasificación , Genoma Viral , Sistemas de Lectura Abierta , Filogenia , Enfermedades de las Plantas , Virus ARN/clasificación , Virus ARN/genética , Virus ARN/aislamiento & purificación , ARN Bicatenario , ARN Viral/genética , ARN Polimerasa Dependiente del ARN , Virus no Clasificados/clasificaciónRESUMEN
The sole member of the Piscihepevirus genus (family Hepeviridae) is cutthroat trout virus (CTV) but recent metatranscriptomic studies have identified numerous fish hepevirus sequences including CTV-2. In the current study, viruses with sequences resembling both CTV and CTV-2 were isolated from salmonids in eastern and western Canada. Phylogenetic analysis of eight full genomes delineated the Canadian CTV isolates into two genotypes (CTV-1 and CTV-2) within the Piscihepevirus genus. Hepevirus genomes typically have three open reading frames but an ORF3 counterpart was not predicted in the Canadian CTV isolates. In vitro replication of a CTV-2 isolate produced cytopathic effects in the CHSE-214 cell line with similar amplification efficiency as CTV. Likewise, the morphology of the CTV-2 isolate resembled CTV, yet viral replication caused dilation of the endoplasmic reticulum lumen which was not previously observed. Controlled laboratory studies exposing sockeye (Oncorhynchus nerka), pink (O. gorbuscha), and chinook salmon (O. tshawytscha) to CTV-2 resulted in persistent infections without disease and mortality. Infected Atlantic salmon (Salmo salar) and chinook salmon served as hosts and potential reservoirs of CTV-2. The data presented herein provides the first in vitro and in vivo characterization of CTV-2 and reveals greater diversity of piscihepeviruses extending the known host range and geographic distribution of CTV viruses.
Asunto(s)
Enfermedades de los Peces/virología , Hepevirus/clasificación , Hepevirus/genética , Hepevirus/aislamiento & purificación , Animales , Canadá , Genotipo , Hepevirus/patogenicidad , Infección Persistente/virología , Filogenia , Salmo salar/virología , Salmón/virología , Trucha , Virulencia , Virus no Clasificados/clasificación , Virus no Clasificados/genética , Virus no Clasificados/aislamiento & purificación , Virus no Clasificados/patogenicidadRESUMEN
A novel clade of RNA viruses was identified in the mammalian gastrointestinal tract by next-generation sequencing. Phylogenetically, these viruses are related to the genera Tombusviridae (plant viruses) and Flaviviridae, which includes mammalian, avian and insect hosts. Named in line with their characterization as stool-associated Tombus-like viruses, it is unclear if statoviruses infect mammals or are dietary in origin. Here, metagenomic sequencing of faecal material collected from a 10-week-old calf with enteric disease found that 20â% of the reads mapped to a de novo-assembled 4 kb contig with homology to statoviruses. Phylogenetic analysis of the statovirus genome found a clear evolutionary relationship with statovirus A, but, with only 47â% similarity, we propose that the statovirus sequence presents a novel species, statovirus F. A TaqMan PCR targeting statovirus F performed on faecal material found a cycle threshold of 11, suggesting a high titre of virus shed from the calf with enteric disease. A collection of 48 samples from bovine enteric disease diagnostic submissions were assayed by PCR to investigate statovirus F prevalence and 6 of 48 (12.5â%) were positive. An ELISA to detect antibodies to the coat protein found that antibodies to statovirus F were almost ubiquitous in bovine serum. Combined, the PCR and ELISA results suggest that statovirus F commonly infects cattle. Further research is needed to elucidate the aetiological significance of statovirus infection.
Asunto(s)
Enfermedades de los Bovinos/virología , Heces/virología , Tracto Gastrointestinal/virología , Enfermedades Intestinales/veterinaria , Enfermedades Intestinales/virología , Infecciones por Virus ARN/veterinaria , Virus ARN/clasificación , Virus ARN/aislamiento & purificación , Animales , Anticuerpos Antivirales/sangre , Bovinos , Secuenciación de Nucleótidos de Alto Rendimiento , Metagenoma , Filogenia , Infecciones por Virus ARN/virología , Virus ARN/genética , Virus ARN/fisiología , Virus no Clasificados/clasificación , Virus no Clasificados/genética , Virus no Clasificados/aislamiento & purificación , Virus no Clasificados/fisiologíaRESUMEN
We report the first emaravirus on an endemic plant of Aotearoa New Zealand that is, to the best of our knowledge, the country's first endemic virus characterised associated with an indigenous plant. The new-to-science virus was identified in the endemic karaka tree (Corynocarpus laevigatus), and is associated with chlorotic leaf spots, and possible feeding sites of the monophagous endemic karaka gall mite. Of the five negative-sense RNA genomic segments that were fully sequenced, four (RNA 1-4) had similarity to other emaraviruses while RNA 5 had no similarity with other viral proteins. A detection assay developed to amplify any of the five RNAs in a single assay was used to determine the distribution of the virus. The virus is widespread in the Auckland area, particularly in mature trees at Okahu Bay, with only occasional reports elsewhere in the North Island. Phylogenetic analysis revealed that its closest relatives are pear chlorotic leaf spot-associated virus and chrysanthemum mosaic-associated virus, which form a unique clade within the genus Emaravirus. Based on the genome structure, we propose this virus to be part of the family Emaravirus, but with less than 50% amino acid similarity to the closest relatives in the most conserved RNA 1, it clearly is a novel species. In consultation with mana whenua (indigenous Maori authority over a territory and its associated treasures), we propose the name Karaka Okahu purepure virus in te reo Maori (the Maori language) to reflect the tree from which it was isolated (karaka), a place where the virus is prevalent (Okahu), and the spotted symptom (purepure, pronounced pooray pooray) that this endemic virus appears to cause.
Asunto(s)
Genoma Viral , Enfermedades de las Plantas/virología , Virus de Plantas/clasificación , Virus de Plantas/genética , Virus no Clasificados/clasificación , Virus no Clasificados/genética , Enfermedades Endémicas , Nueva Zelanda , Filogenia , Virus de Plantas/aislamiento & purificación , ARN Viral/genética , Proteínas Virales/genética , Virus no Clasificados/aislamiento & purificaciónRESUMEN
This article reports the changes to virus taxonomy approved and ratified by the International Committee on Taxonomy of Viruses (ICTV) in March 2021. The entire ICTV was invited to vote on 290 taxonomic proposals approved by the ICTV Executive Committee at its meeting in October 2020, as well as on the proposed revision of the International Code of Virus Classification and Nomenclature (ICVCN). All proposals and the revision were ratified by an absolute majority of the ICTV members. Of note, ICTV mandated a uniform rule for virus species naming, which will follow the binomial 'genus-species' format with or without Latinized species epithets. The Study Groups are requested to convert all previously established species names to the new format. ICTV has also abolished the notion of a type species, i.e., a species chosen to serve as a name-bearing type of a virus genus. The remit of ICTV has been clarified through an official definition of 'virus' and several other types of mobile genetic elements. The ICVCN and ICTV Statutes have been amended to reflect these changes.
Asunto(s)
Clasificación/métodos , Filogenia , Virus no Clasificados/clasificación , Virus/clasificación , Cooperación Internacional , Viroides/clasificación , Virus/genética , Virus/aislamiento & purificación , Virus no Clasificados/genética , Virus no Clasificados/aislamiento & purificaciónRESUMEN
Hollyhock (Alcea rosea, family Malvaceae) is an ornamental plant grown widely in gardens across South Asia. In a bed of ornamental plants near the village of Chakri (Punjab Province, Pakistan) in 2014, hollyhock plants showing two distinct symptom types were identified: yellow vein mosaic and leaf crumple. PCR amplification with universal primers amplified a begomovirus from separate nucleic acid extracts of single plants of each type but amplified a betasatellite only from the plant with the yellow vein mosaic symptoms. No potential begomovirus DNA B component or alphasatellite could be identified in either sample. After cloning, the genome sequences of two viruses, one from a plant of each symptom type, were determined and shown to share 99.9% nucleotide sequence identity with each other but less than 91% nucleotide sequence identity with all previously characterized begomoviruses, with the highest identity (90%) to an isolate of pedilanthus leaf curl virus (PeLCV). This indicates that the two hollyhock plants were infected with a newly identified begomovirus for which the name "hollyhock vein yellowing virus" (HoVYV) is proposed. HoVYV likely has a recombinant origin. The betasatellite showed the highest nucleotide sequence identity to an isolate of cotton leaf curl Multan betasatellite (CLCuMuB), a betasatellite associated with cotton leaf curl disease across Pakistan and northwestern India. These findings add to the diversity of known begomoviruses in South Asia and again highlight the role of hollyhock as a reservoir of the cotton leaf curl begomovirus betasatellite complex. The results also suggest that the yellow vein mosaic symptoms in hollyhock are due to the betasatellite rather than the virus.
Asunto(s)
Begomovirus/clasificación , Begomovirus/genética , Malvaceae/virología , Virus de Plantas/clasificación , Virus de Plantas/genética , Secuenciación Completa del Genoma , Secuencia de Bases , Begomovirus/aislamiento & purificación , Virus ADN/genética , ADN Viral/genética , Pakistán , Filogenia , Enfermedades de las Plantas/virología , Virus de Plantas/aislamiento & purificación , Virus no Clasificados/clasificación , Virus no Clasificados/genética , Virus no Clasificados/aislamiento & purificaciónRESUMEN
A novel geminivirus was identified in France and Spain in asymptomatic plants of white clover (Trifolium repens) and shrub medick (Medicago arborea). Its genome has the hallmarks of a capulavirus, and its relationship to other capulaviruses was confirmed by phylogenetic analysis. White clover isolates formed a tight cluster in the phylogenetic tree, while shrub medick isolates formed two distinct, more divergent groups with sequence identity values close to the species cutoff. These three groups have likely participated in recombination events involving alfalfa leaf curl virus and French bean severe leaf curl virus. The name "trifolium virus 1" (TrV1) is proposed for this new Capulavirus. Three TrV1 genotypes (TrV1-A, TrV1-B, and TrV1-C) were clearly distinguished.
Asunto(s)
Filogenia , Trifolium/virología , Virus no Clasificados/clasificación , Virus no Clasificados/genética , Virus no Clasificados/aislamiento & purificación , Secuencia de Aminoácidos , Biodiversidad , Virus ADN/genética , Fabaceae/virología , Geminiviridae/clasificación , Geminiviridae/genética , Geminiviridae/aislamiento & purificación , Genotipo , Sistemas de Lectura Abierta , Enfermedades de las Plantas/virología , Análisis de Secuencia de ADNRESUMEN
Kaumoebavirus infects the amoeba Vermamoeba vermiformis and has recently been described as a distant relative of the African swine fever virus. To characterize the diversity and evolution of this novel viral genus, we report here on the isolation and genome sequencing of a second strain of Kaumoebavirus, namely LCC10. Detailed analysis of the sequencing data suggested that its 362-Kb genome is linear with covalently closed hairpin termini, so that DNA forms a single continuous polynucleotide chain. Comparative genomic analysis indicated that although the two sequenced Kaumoebavirus strains share extensive gene collinearity, 180 predicted genes were either gained or lost in only one genome. As already observed in another distant relative, i.e., Faustovirus, which infects the same host, the center and extremities of the Kaumoebavirus genome exhibited a higher rate of sequence divergence and the major capsid protein gene was colonized by type-I introns. A possible role of the Vermamoeba host in the genesis of these evolutionary traits is hypothesized. The Kaumoebavirus genome exhibited a significant gene strand bias over the two-third of genome length, a feature not seen in the other members of the "extended Asfarviridae" clade. We suggest that this gene strand bias was induced by a putative single origin of DNA replication located near the genome extremity that imparted a selective force favoring the genes positioned on the leading strand.
Asunto(s)
Asfarviridae/genética , Genoma Viral , Virus Gigantes/genética , Virus no Clasificados/genética , Asfarviridae/clasificación , Proteínas de la Cápside/genética , Replicación del ADN , Virus ADN/clasificación , Virus ADN/genética , Virus ADN/aislamiento & purificación , ADN Viral/química , ADN Viral/genética , ADN Viral/metabolismo , Evolución Molecular , Genes Virales , Virus Gigantes/clasificación , Virus Gigantes/aislamiento & purificación , Virus Gigantes/ultraestructura , Lobosea/virología , Filogenia , Aguas del Alcantarillado/virología , Proteínas Virales/genética , Virus no Clasificados/aislamiento & purificación , Virus no Clasificados/ultraestructuraRESUMEN
Lactarius fungi belong to the Russulaceae family and have an important ecological role as ectomycorrhizal symbionts of coniferous and deciduous trees. Two Lactarius species, L. tabidus and L. rufus have been shown to harbor bisegmented dsRNA viruses belonging to an unclassified virus group including the mutualistic Curvularia thermal tolerance virus (CThTV). In this study, we characterized the first complete genome sequences of these viruses designated as Lactarius tabidus RNA virus 1 (LtRV1) and Lactarius rufus RNA virus 1 (LrRV1), both of which included two genome segments of 2241 and 2049 bp. We also analyzed spatial distribution and sequence diversity of the viruses in sixty host strains at two forest sites, and showed that the viruses are species-specific at sites where both host species co-occur. We also found that single virus isolates inhabited several different conspecific host strains, and were involved in persistent infections during up to eight years.
Asunto(s)
Basidiomycota/virología , Virus ARN , Árboles , Virus no Clasificados , Finlandia , Bosques , Filogenia , Virus ARN/clasificación , Virus ARN/aislamiento & purificación , Especificidad de la Especie , Árboles/microbiología , Árboles/virología , Virus no Clasificados/clasificación , Virus no Clasificados/aislamiento & purificaciónRESUMEN
Faustovirus is a recently discovered genus of large DNA virus infecting the amoeba Vermamoeba vermiformis, which is phylogenetically related to Asfarviridae. To better understand the diversity and evolution of this viral group, we sequenced six novel Faustovirus strains, mined published metagenomic datasets and performed a comparative genomic analysis. Genomic sequences revealed three consistent phylogenetic groups, within which genetic diversity was moderate. The comparison of the major capsid protein (MCP) genes unveiled between 13 and 18 type-I introns that likely evolved through a still-active birth and death process mediated by intron-encoded homing endonucleases that began before the Faustovirus radiation. Genome-wide alignments indicated that despite genomes retaining high levels of gene collinearity, the central region containing the MCP gene together with the extremities of the chromosomes evolved at a faster rate due to increased indel accumulation and local rearrangements. The fluctuation of the nucleotide composition along the Faustovirus (FV) genomes is mostly imprinted by the consistent nucleotide bias of coding sequences and provided no evidence for a single DNA replication origin like in circular bacterial genomes.
Asunto(s)
Evolución Molecular , Genoma Viral , Genómica , Virus no Clasificados/genética , Asfarviridae/genética , Proteínas de la Cápside/genética , Virus ADN/genética , ADN Viral/genética , Metagenómica , Filogenia , Ensamble de Virus , Virus no Clasificados/clasificación , Virus no Clasificados/aislamiento & purificaciónRESUMEN
Microbes trapped in permanently frozen paleosoils (permafrost) are the focus of increasing research in the context of global warming. Our previous investigations led to the discovery and reactivation of two Acanthamoeba-infecting giant viruses, Mollivirus sibericum and Pithovirus sibericum, from a 30,000-year old permafrost layer. While several modern pithovirus strains have since been isolated, no contemporary mollivirus relative was found. We now describe Mollivirus kamchatka, a close relative to M. sibericum, isolated from surface soil sampled on the bank of the Kronotsky River in Kamchatka, Russian Federation. This discovery confirms that molliviruses have not gone extinct and are at least present in a distant subarctic continental location. This modern isolate exhibits a nucleocytoplasmic replication cycle identical to that of M. sibericum Its spherical particle (0.6 µm in diameter) encloses a 648-kb GC-rich double-stranded DNA genome coding for 480 proteins, of which 61% are unique to these two molliviruses. The 461 homologous proteins are highly conserved (92% identical residues, on average), despite the presumed stasis of M. sibericum for the last 30,000 years. Selection pressure analyses show that most of these proteins contribute to virus fitness. The comparison of these first two molliviruses clarify their evolutionary relationship with the pandoraviruses, supporting their provisional classification in a distinct family, the Molliviridae, pending the eventual discovery of intermediary missing links better demonstrating their common ancestry.IMPORTANCE Virology has long been viewed through the prism of human, cattle, or plant diseases, leading to a largely incomplete picture of the viral world. The serendipitous discovery of the first giant virus visible under a light microscope (i.e., >0.3 µm in diameter), mimivirus, opened a new era of environmental virology, now incorporating protozoan-infecting viruses. Planet-wide isolation studies and metagenome analyses have shown the presence of giant viruses in most terrestrial and aquatic environments, including upper Pleistocene frozen soils. Those systematic surveys have led authors to propose several new distinct families, including the Mimiviridae, Marseilleviridae, Faustoviridae, Pandoraviridae, and Pithoviridae We now propose to introduce one additional family, the Molliviridae, following the description of M. kamchatka, the first modern relative of M. sibericum, previously isolated from 30,000-year-old arctic permafrost.
Asunto(s)
Virus Gigantes/clasificación , Virus Gigantes/genética , Virus Gigantes/aislamiento & purificación , Filogenia , Acanthamoeba/virología , Virus ADN/clasificación , Virus ADN/genética , Genoma Viral , Genómica , Virus Gigantes/ultraestructura , Mimiviridae/clasificación , Mimiviridae/genética , Federación de Rusia , Microbiología del Suelo , Virión/genética , Virión/ultraestructura , Virus no Clasificados/clasificación , Virus no Clasificados/genética , Virus no Clasificados/aislamiento & purificaciónRESUMEN
The in silico analyses of 109 replication-competent genomic DNA sequences isolated from cow milk and its products (97 in the bovine meat and milk factors 2 group - BMMF2, and additional 4 in BMMF1) seems to place these in a specific class of infectious agents spanning between bacterial plasmid and circular ssDNA viruses. Satellite-type small plasmids with partial homology to larger genomes, were also isolated in both groups. A member of the BMMF1 group H1MBS.1 was recovered in a distinctly modified form from colon tissue by laser microdissection. Although the evolutionary origin is unknown, it draws the attention to the existence of a hitherto unrecognized, broad spectrum of potential pathogens. Indirect hints to the origin and structure of our isolates, as well as to their replicative behaviour, result from parallels drawn to the Hepatitis deltavirus genome structure and replication.
Asunto(s)
Neoplasias del Colon/virología , Virus ADN/aislamiento & purificación , Productos Lácteos/virología , Leche/virología , Suero/virología , Virus no Clasificados/aislamiento & purificación , Animales , Bovinos , Virus ADN/genética , Humanos , Análisis de Secuencia de ADN , Homología de Secuencia de Ácido Nucleico , Virus no Clasificados/genéticaRESUMEN
In this study, we report the molecular characterization of a novel mycovirus in a phytopathogenic fungus of the species Colletotrichum gloeosporioides, which we named "Colletotrichum gloeosporioides RNA virus 1" (CgRV1). The virus has a dsRNA genome of 2,975 bp and possesses two non-overlapping open reading frames (ORFs 1 and 2). The smaller ORF1 encodes a protein of unknown function, and the larger ORF2 encodes the RNA-dependent RNA polymerase (RdRp). Phylogenetic analysis based on the RdRp sequence showed that CgRV1 clustered with and is closely related to unclassified mycoviruses that are distinct from members of the family Partitiviridae.
Asunto(s)
Colletotrichum/virología , Virus Fúngicos/genética , Genoma Viral/genética , Virus no Clasificados/genética , Secuencia de Aminoácidos , Virus Fúngicos/aislamiento & purificación , Sistemas de Lectura Abierta/genética , Enfermedades de las Plantas/microbiología , ARN Viral/genética , Virus no Clasificados/aislamiento & purificaciónRESUMEN
In 2013, seven virus strains were isolated from Culex tritaeniorhynchus and C. quinquefasciatus collected in Guangxi Province, China. The viruses caused cytopathological effects (CPE) only in C6/36 cells, and not in mammalian cells, e.g. BHK21 and Vero cells. Comparative genomic analysis revealed that the genomes of the seven virus strains ranged from 9589 to 9595 nt with G+C contents of 37.51-37.59%, and nucleotide sequence similarity > 99.8%. The nucleotide sequence homology of the seven virus strains with a Tanay virus isolate was 79.8-80.0%, while the corresponding amino acid homology of ORF1, ORF2, and ORF3 was 79.8-80, 73.9-4.6, and 93.4-94.3%, respectively. In phylogenetic analysis, based on the RdRP amino acid sequence, the seven virus strains were located on the same evolutionary branch, yet distinct from Tanay virus isolated from the Philippines. These results indicated that the seven virus strains isolated from Guangxi represent a new Tanay virus, "Tanay-like virus", within the genus Sandewavirus of the newly proposed taxon Negevirus. This was the first isolation of Tanay virus in China.
Asunto(s)
Culex/virología , Virus no Clasificados/genética , Animales , Composición de Base , Secuencia de Bases , China , Chlorocebus aethiops , Genoma Viral , Datos de Secuencia Molecular , Sistemas de Lectura Abierta , Filogenia , Células Vero , Virus no Clasificados/clasificación , Virus no Clasificados/aislamiento & purificaciónRESUMEN
Terrestrial orchids represent a symbiotic union between plants and mycorrhizal fungi. This study describes the occurrence and nature of viruses associated with one population of wild Pterostylis sanguinea orchids, including their fungal symbionts, over two consecutive years. A generic sequencing approach, which combined dsRNA-enrichment from plant and mycelial tissues, random amplification and high throughput shotgun sequencing was used to identify novel viruses. The majority of the virus-like sequences represent partial genomes, and their identification is based solely on de novo assembly of sequencing data. In orchid leaf tissues we found three isolates of a novel totivirus and an unclassified virus; both resemble fungus-infecting viruses. Two isolates of Ceratobasidium sp that were isolated from orchid underground stems contained at least 20 viruses, 16 of which were previously described as alphapartitiviruses and betapartitiviruses. A novel hypovirus and a mitovirus were genetically distant from existing members of the genera and did not readily fit into recognised subgroups.
Asunto(s)
Virus Fúngicos/genética , Micorrizas/virología , Orchidaceae/microbiología , Virus ARN/genética , Simbiosis/fisiología , Totivirus/genética , Virus no Clasificados/genética , Australia , Virus Fúngicos/clasificación , Virus Fúngicos/aislamiento & purificación , Secuenciación de Nucleótidos de Alto Rendimiento , Filogenia , Hojas de la Planta/microbiología , Virus ARN/clasificación , Virus ARN/aislamiento & purificación , ARN Bicatenario/genética , Análisis de Secuencia de ADN , Totivirus/clasificación , Totivirus/aislamiento & purificación , Virus no Clasificados/clasificación , Virus no Clasificados/aislamiento & purificaciónRESUMEN
In this study, we report the isolation of a new giant virus found in sewage water from the southern area of Jeddah (Saudi Arabia), with morphological and genomic resemblance to Faustoviruses. This new giant virus, named Kaumoebavirus, was obtained from co-culture with Vermamoeba vermiformis, an amoeboid protozoa considered to be of special interest to human health and the environment. This new virus has ~250 nm icosahedral capsids and a 350,731 bp DNA genome length. The genome of Kaumoebavirus has a coding density of 86%, corresponding to 465 genes. Most of these genes (59%) are closely related to genes from members of the proposed order Megavirales, and the best matches to its proteins with other members of the Megavirales are Faustoviruses (43%) and Asfarviruses (23%). Unsurprisingly, phylogenetic reconstruction places Kaumoebavirus as a distant relative of Faustoviruses and Asfarviruses.
Asunto(s)
Virus Gigantes/clasificación , Virus Gigantes/aislamiento & purificación , Virus no Clasificados/clasificación , Virus no Clasificados/aislamiento & purificación , Técnicas de Cocultivo , ADN Viral/química , ADN Viral/genética , Genes Virales , Genoma Viral , Virus Gigantes/ultraestructura , Lobosea/crecimiento & desarrollo , Lobosea/virología , Arabia Saudita , Análisis de Secuencia de ADN , Aguas del Alcantarillado/virología , Virión/ultraestructura , Virus no Clasificados/ultraestructuraRESUMEN
Recently, gemycircularviruses have been found in humans and various species of animals. Here, a novel gemycircularvirus named Ch-zjrat-01 from blood samples of experimental rats was characterized. The novel gemycircularvirus encodes two major proteins, including a capsid protein (Cap) and a replication-associated protein (Rep). Phylogenetic analysis based on the amino acid sequence of Rep indicated that Ch-zjrat-01 clusters with two gemycircularviruses discovered from bird (KF371635) and mosquito (HQ335086), sharing 48.7 and 49.4 % sequence identities with them, respectively.
Asunto(s)
Sangre/virología , Virus no Clasificados/clasificación , Virus no Clasificados/aislamiento & purificación , Animales , Animales de Laboratorio , Análisis por Conglomerados , Genoma Viral , Datos de Secuencia Molecular , Filogenia , ARN Viral/genética , Ratas , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido , Proteínas Virales/genética , Virus no Clasificados/genéticaRESUMEN
Fungal viruses (mycoviruses) often have a significant impact not only on phenotypic expression of the host fungus but also on higher order biological interactions, e.g., conferring plant stress tolerance via an endophytic host fungus. Arbuscular mycorrhizal (AM) fungi in the phylum Glomeromycota associate with most land plants and supply mineral nutrients to the host plants. So far, little information about mycoviruses has been obtained in the fungi due to their obligate biotrophic nature. Here we provide a technical breakthrough, "two-step strategy" in combination with deep-sequencing, for virological study in AM fungi; dsRNA is first extracted and sequenced using material obtained from highly productive open pot culture, and then the presence of viruses is verified using pure material produced in the in vitro monoxenic culture. This approach enabled us to demonstrate the presence of several viruses for the first time from a glomeromycotan fungus.
Asunto(s)
Secuenciación de Nucleótidos de Alto Rendimiento/métodos , Micorrizas/virología , Virus no Clasificados/genética , Virus no Clasificados/aislamiento & purificación , Micorrizas/crecimiento & desarrollo , Filogenia , ARN Bicatenario/aislamiento & purificación , Esporas Fúngicas/virologíaRESUMEN
The complete nucleotide sequence of Alternaria longipes dsRNA virus 1 (AlRV1), a novel double-stranded RNA (dsRNA) mycovirus, was determined and analyzed. AlRV1-HN28 contains a single dsRNA genome segment 3415 base pairs in length (excluding the 3' poly(A) tail) and was predicted to contain two discontiguous open reading frames (ORFs, ORF A and ORF B). The 5'-proximal ORF A (1182 nt) potentially encodes a protein of 394 amino acids (aa) with a predicted molecular mass of 43 kDa; this protein showed no significant similarities to any other sequences in any of the NCBI protein databases. The 3'-proximal ORF B (1737 nt) encodes a protein of 579 aa with a predicted molecular mass of 65 kDa; this protein sequence shares similarities with the conserved domains of RNA-dependent RNA polymerases of other mycoviruses. Phylogenetic analysis indicated that AlRV1-HN28 was closely related to four other unclassified viruses, which suggests that the AlRV1-HN28 isolated from Alternaria longipes may belong to a new family of dsRNA mycoviruses. This is the first report of the full-length nucleotide sequence of a mycovirus that infects Alternaria longipes.
Asunto(s)
Alternaria/virología , Genoma Viral/genética , Virus ARN/genética , Virus no Clasificados/genética , Regiones no Traducidas 3'/genética , Regiones no Traducidas 5'/genética , Secuencia de Aminoácidos , Secuencia de Bases , Sistemas de Lectura Abierta/genética , Filogenia , ARN Bicatenario , ARN Viral/genética , ARN Polimerasa Dependiente del ARN/genética , Análisis de Secuencia de ARN , Proteínas Virales/genética , Virus no Clasificados/aislamiento & purificaciónRESUMEN
In this study, we identified a novel virus from gentian (Gentiana triflora) that causes ring-spots on ovaries. Furthermore, the virus causes unusual symptoms, ring-spots that appear specifically on the outer surface of the ovarian wall after pollination. Pollen grains carrying the virus were used to infect host plants by hand-pollination. RNA extracted from purified virions indicated that the virus had two segments, RNA1 and RNA2. The full-length cDNA sequence indicated that RNA1 had two ORFs: ORF1 had methyltransferase and helicase motifs, and ORF2 had an RNA-dependent RNA polymerase motif. RNA2 had five ORFs encoding a coat protein, triple gene block proteins 1-3 and a cysteine-rich protein. The length of RNA1 was 5519 bases and that of RNA2 was 3810 bases not including a polyU/polyA region between the first and second ORFs. Viral RNA does not have a polyA tail at the 3' end. Sequence similarity and phylogenetic analysis suggested that the virus is closely related to members of the genera Pecluvirus and Hordeivirus but distinct from them. These combined results suggest that the causal agent inducing ring-spot symptoms on gentian ovaries is a new virus belonging to the family Virgaviridae but not to any presently known genus. We tentatively name the virus gentian ovary ring-spot virus.