RESUMEN
The plasticity of the xylem and its associated hydraulic properties play crucial roles in plant acclimation to environmental changes, with vessel diameter (Dv) being the most functionally prominent trait. While the effects of external environmental factors on xylem formation and Dv are not fully understood, the endogenous hormones indole-3-acetic acid (IAA) and abscisic acid (ABA) are known to play significant signalling roles under stress conditions. This study investigates how these hormones impact Dv under various environmental changes. Experiments were conducted in maize plants subjected to drought, soil salinity, and high CO2 concentration treatments. We found that drought and soil salinity significantly reduced Dv at the same stem internode, while an elevated CO2 concentration can mitigate this decrease in Dv. Remarkably, significant negative correlations were observed between Dv and the contents of IAA and ABA when considering the different treatments. Moreover, appropriate foliar application of either IAA or ABA on well-watered and stressed plants led to a decrease in Dv, while the application of corresponding inhibitors resulted in an increase in Dv. This finding underscores the causal relationship between Dv and the levels of both IAA and ABA, offering a promising approach to manipulating xylem vessel size.
Asunto(s)
Ácido Abscísico , Ácidos Indolacéticos , Tallos de la Planta , Estrés Fisiológico , Xilema , Zea mays , Zea mays/efectos de los fármacos , Zea mays/fisiología , Zea mays/metabolismo , Zea mays/crecimiento & desarrollo , Ácido Abscísico/metabolismo , Ácido Abscísico/farmacología , Ácidos Indolacéticos/metabolismo , Ácidos Indolacéticos/farmacología , Xilema/efectos de los fármacos , Xilema/fisiología , Xilema/metabolismo , Estrés Fisiológico/efectos de los fármacos , Tallos de la Planta/efectos de los fármacos , Tallos de la Planta/fisiología , Tallos de la Planta/metabolismo , Reguladores del Crecimiento de las Plantas/metabolismo , Reguladores del Crecimiento de las Plantas/farmacología , Sequías , Suelo/química , Salinidad , Dióxido de Carbono/metabolismo , Dióxido de Carbono/farmacologíaRESUMEN
The bundle sheath cell (BSC) layer tightly enveloping the xylem throughout the leaf is recognized as a major signal-perceiving "valve" in series with stomata, regulating leaf hydraulic conductance (Kleaf) and thereby radial water flow via the transpiring leaf. The BSC blue light (BL) signaling pathway increases Kleaf and the underlying BSC water permeability. Here, we explored the hypothesis that BSCs also harbor a Kleaf-downregulating signaling pathway related to the stress phytohormone abscisic acid (ABA). We employed fluorescence imaging of xylem sap in detached leaves and BSC protoplasts from different genotypes of Arabidopsis (Arabidopsis thaliana) plants, using pH and membrane potential probes to monitor physiological responses to ABA and BL in combination with pharmacological agents. We found that BL-enhanced Kleaf required elevated BSC cytosolic Ca2+. ABA inhibited BL-activated xylem-sap-acidifying BSC H+-ATPase AHA2 (Arabidopsis H+-ATPase 2), resulting in depolarized BSCs and alkalinized xylem sap. ABA also stimulated BSC vacuolar H+-ATPase (VHA), which alkalinized the BSC cytosol. Each pump stimulation, AHA2 by BL and VHA by ABA (under BL), also required Ca2+. ABA stimulated VHA in the dark depending on Ca2+, but only in an alkaline external medium. Taken together with earlier findings on the pH sensitivity of BSC osmotic water permeability (i.e. aquaporin activity), our results suggest a Ca2+-dependent and pH-mediated causative link between the BL- and ABA-regulated activities of two BSC H+-ATPases and Kleaf.
Asunto(s)
Ácido Abscísico , Arabidopsis , Luz , Hojas de la Planta , ATPasas de Translocación de Protón , Xilema , Ácido Abscísico/metabolismo , Ácido Abscísico/farmacología , Arabidopsis/fisiología , Arabidopsis/efectos de los fármacos , ATPasas de Translocación de Protón/metabolismo , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/fisiología , Xilema/efectos de los fármacos , Xilema/fisiología , Proteínas de Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Agua/metabolismo , Concentración de Iones de Hidrógeno , Calcio/metabolismo , Transducción de Señal , Luz AzulRESUMEN
Sea-level rise is one of the most critical challenges facing coastal ecosystems under climate change. Observations of elevated tree mortality in global coastal forests are increasing, but important knowledge gaps persist concerning the mechanism of salinity stress-induced nonhalophytic tree mortality. We monitored progressive mortality and associated gas exchange and hydraulic shifts in Sitka-spruce (Picea sitchensis) trees located within a salinity gradient under an ecosystem-scale change of seawater exposure in Washington State, USA. Percentage of live foliated crown (PLFC) decreased and tree mortality increased with increasing soil salinity during the study period. A strong reduction in gas exchange and xylem hydraulic conductivity (Ks) occurred during tree death, with an increase in the percentage loss of conductivity (PLC) and turgor loss point (πtlp). Hydraulic and osmotic shifts reflected that hydraulic function declined from seawater exposure, and dying trees were unable to support osmotic adjustment. Constrained gas exchange was strongly related to hydraulic damage at both stem and leaf levels. Significant correlations between foliar sodium (Na+) concentration and gas exchange and key hydraulic parameters (Ks, PLC, and πtlp) suggest that cellular injury related to the toxic effects of ion accumulation impacted the physiology of these dying trees. This study provides evidence of toxic effects on the cellular function that manifests in all aspects of plant functioning, leading to unfavourable osmotic and hydraulic conditions.
Asunto(s)
Cambio Climático , Picea/fisiología , Estrés Salino , Agua de Mar/efectos adversos , Árboles/fisiología , Xilema/efectos de los fármacos , Washingtón , Xilema/fisiologíaRESUMEN
The role of reactive oxygen species (ROS) in ABA-induced increase in hydraulic conductivity was hypothesized to be dependent on an increase in aquaporin water channel (AQP) abundance. Single ABA application or its combination with ROS manipulators (ROS scavenger ascorbic acid and NADPH oxidase inhibitor diphenyleneiodonium chloride (DPI)) were studied on detached roots of barley plants. We measured the osmotically driven flow rate of xylem sap and calculated root hydraulic conductivity. In parallel, immunolocalization of ABA and HvPIP2;2 AQPs was performed with corresponding specific antibodies. ABA treatment increased the flow rate of xylem, root hydraulic conductivity and immunostaining for ABA and HvPIP2;2, while the addition of antioxidants prevented the effects of this hormone. The obtained results confirmed the involvement of ROS in ABA effect on hydraulic conductivity, in particular, the importance of H2O2 production by ABA-treated plants for the effect of this hormone on AQP abundance.
Asunto(s)
Ácido Abscísico/farmacología , Acuaporinas/metabolismo , Ósmosis , Proteínas de Plantas/metabolismo , Raíces de Plantas/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Antioxidantes/farmacología , Ácido Ascórbico/farmacología , Inhibidores Enzimáticos/farmacología , Hordeum/efectos de los fármacos , Hordeum/metabolismo , NADPH Oxidasas/antagonistas & inhibidores , NADPH Oxidasas/metabolismo , Compuestos Onio/farmacología , Raíces de Plantas/efectos de los fármacos , Xilema/efectos de los fármacos , Xilema/metabolismoRESUMEN
Secondary growth relies on precise and specialized transcriptional networks that determine cell division, differentiation, and maturation of xylem cells. We identified a novel role for the ethylene-induced Populus Ethylene Response Factor PtERF85 (Potri.015G023200) in balancing xylem cell expansion and secondary cell wall (SCW) formation in hybrid aspen (Populus tremula x tremuloides). Expression of PtERF85 is high in phloem and cambium cells and during the expansion of xylem cells, while it is low in maturing xylem tissue. Extending PtERF85 expression into SCW forming zones of woody tissues through ectopic expression reduced wood density and SCW thickness of xylem fibers but increased fiber diameter. Xylem transcriptomes from the transgenic trees revealed transcriptional induction of genes involved in cell expansion, translation, and growth. The expression of genes associated with plant vascular development and the biosynthesis of SCW chemical components such as xylan and lignin, was down-regulated in the transgenic trees. Our results suggest that PtERF85 activates genes related to xylem cell expansion, while preventing transcriptional activation of genes related to SCW formation. The importance of precise spatial expression of PtERF85 during wood development together with the observed phenotypes in response to ectopic PtERF85 expression suggests that PtERF85 contributes to the transition of fiber cells from elongation to secondary cell wall deposition.
Asunto(s)
Pared Celular/metabolismo , Proteínas de Plantas/metabolismo , Populus/metabolismo , Xilema/metabolismo , Cámbium/metabolismo , Pared Celular/efectos de los fármacos , Regulación hacia Abajo/efectos de los fármacos , Etilenos/farmacología , Redes Reguladoras de Genes , Lignina/metabolismo , Floema/metabolismo , Filogenia , Proteínas de Plantas/clasificación , Proteínas de Plantas/genética , Populus/crecimiento & desarrollo , Regulación hacia Arriba/efectos de los fármacos , Madera/crecimiento & desarrollo , Madera/metabolismo , Xilema/citología , Xilema/efectos de los fármacosRESUMEN
Brassinosteroids (BRs) are known to be essential regulators for wood formation in herbaceous plants and poplar, but their roles in secondary growth and xylem development are still not well-defined, especially in pines. Here, we treated Pinus massoniana seedlings with different concentrations of exogenous BRs, and assayed the effects on plant growth, xylem development, endogenous phytohormone contents and gene expression within stems. Application of exogenous BR resulted in improving development of xylem more than phloem, and promoting xylem development in a dosage-dependent manner in a certain concentration rage. Endogenous hormone determination showed that BR may interact with other phytohormones in regulating xylem development. RNA-seq analysis revealed that some conventional phenylpropanoid biosynthesis- or lignin synthesis-related genes were downregulated, but the lignin content was elevated, suggesting that new lignin synthesis pathways or other cell wall components should be activated by BR treatment in P. massoniana. The results presented here reveal the foundational role of BRs in regulating plant secondary growth, and provide the basis for understanding molecular mechanisms of xylem development in P. massoniana.
Asunto(s)
Brasinoesteroides/farmacología , Pinus/metabolismo , Xilema/metabolismo , Brasinoesteroides/metabolismo , Pared Celular/metabolismo , Regulación de la Expresión Génica de las Plantas/genética , Lignina/metabolismo , Floema/efectos de los fármacos , Floema/metabolismo , Pinus/crecimiento & desarrollo , Reguladores del Crecimiento de las Plantas/metabolismo , Reguladores del Crecimiento de las Plantas/farmacología , Proteínas de Plantas/genética , Plantones/crecimiento & desarrollo , Plantones/metabolismo , Madera/genética , Xilema/efectos de los fármacos , Xilema/crecimiento & desarrolloRESUMEN
Soybean (Glycine max (L.) Merr.) plants form root nodules and fix atmospheric dinitrogen, while also utilizing the combined nitrogen absorbed from roots. In this study, nodulated soybean plants were supplied with 5 mM N nitrate, ammonium, or urea for 3 days, and the changes in metabolite concentrations in the xylem sap and each organ were analyzed. The ureide concentration in the xylem sap was the highest in the control plants that were supplied with an N-free nutrient solution, but nitrate and asparagine were the principal compounds in the xylem sap with nitrate treatment. The metabolite concentrations in both the xylem sap and each organ were similar between the ammonium and urea treatments. Considerable amounts of urea were present in the xylem sap and all the organs among all the treatments. Positive correlations were observed between the ureides and urea concentrations in the xylem sap as well as in the roots and leaves, although no correlations were observed between the urea and arginine concentrations, suggesting that urea may have originated from ureide degradation in soybean plants, possibly in the roots. This is the first finding of the possibility of ureide degradation to urea in the underground organs of soybean plants.
Asunto(s)
Compuestos de Amonio/farmacología , Glycine max/efectos de los fármacos , Glycine max/metabolismo , Nitratos/farmacología , Urea/farmacología , Alantoína/metabolismo , Aminoácidos/metabolismo , Fijación del Nitrógeno/efectos de los fármacos , Nodulación de la Raíz de la Planta/efectos de los fármacos , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/metabolismo , Azúcares/metabolismo , Urea/metabolismo , Xilema/efectos de los fármacos , Xilema/metabolismoRESUMEN
Parasitic plants form vascular connections with host plants for efficient material transport. The haustorium is the responsible organ for host invasion and subsequent vascular connection. After invasion of host tissues, vascular meristem-like cells emerge in the central region of the haustorium, differentiate into tracheary elements and establish a connection, known as a xylem bridge, between parasite and host xylem systems. Despite the importance of this parasitic connection, the regulatory mechanisms of xylem bridge formation are unknown. Here, we show the role of auxin and auxin transporters during the process of xylem bridge formation using an Orobanchaceae hemiparasitic plant, Phtheirospermum japonicum The auxin response marker DR5 has a similar expression pattern to tracheary element differentiation genes in haustoria. Auxin transport inhibitors alter tracheary element differentiation in haustoria, but biosynthesis inhibitors do not, demonstrating the importance of auxin transport during xylem bridge formation. The expression patterns and subcellular localization of PIN family auxin efflux carriers and AUX1/LAX influx carriers correlate with DR5 expression patterns. The cooperative action of auxin transporters is therefore responsible for controlling xylem vessel connections between parasite and host.
Asunto(s)
Arabidopsis/parasitología , Ácidos Indolacéticos/metabolismo , Orobanchaceae/fisiología , Xilema/fisiología , Arabidopsis/metabolismo , Proteínas de Arabidopsis/metabolismo , Transporte Biológico , Glucosiltransferasas/genética , Glucosiltransferasas/metabolismo , Proteínas de Transporte de Membrana/química , Proteínas de Transporte de Membrana/genética , Proteínas de Transporte de Membrana/metabolismo , Orobanchaceae/crecimiento & desarrollo , Orobanchaceae/metabolismo , Fenilacetatos/farmacología , Ftalimidas/farmacología , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Raíces de Plantas/metabolismo , Interferencia de ARN , Receptores del Ligando Inductor de Apoptosis Relacionado con TNF/metabolismo , Xilema/efectos de los fármacos , Xilema/metabolismoRESUMEN
Plants can modify xylem anatomy and hydraulic properties to adjust to water status. Elevated [CO2 ] can increase plant water potential via reduced stomatal conductance and water loss. This raises the question of whether elevated [CO2 ], which thus improves plant water status, will reduce the impacts of soil water deficit on xylem anatomy and hydraulic properties of plants. To analyse the impacts of water and [CO2 ] on maize stem xylem anatomy and hydraulic properties, we exposed potted maize plants to varying [CO2 ] levels (400, 700, 900, and 1,200 ppm) and water levels (full irrigation and deficit irrigation). Results showed that at current [CO2 ], vessel diameter, vessel roundness, stem cross-section area, specific hydraulic conductivity, and vulnerability to embolism decreased under deficit irrigation; yet, these impacts of deficit irrigation were reduced at elevated [CO2 ]. Across all treatments, midday stem water potential was tightly correlated with xylem traits and displayed similar responses. A distinct trade-off between efficiency and safety in stem xylem water transportation in response to water deficit was observed at current [CO2 ] but not observed at elevated [CO2 ]. The results of this study enhance our knowledge of plant hydraulic acclimation under future climate environments and provide insights into trade-offs in xylem structure and function.
Asunto(s)
Dióxido de Carbono/farmacología , Tallos de la Planta/fisiología , Agua/metabolismo , Xilema/anatomía & histología , Zea mays/fisiología , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/fisiología , Tallos de la Planta/efectos de los fármacos , Xilema/efectos de los fármacos , Zea mays/efectos de los fármacos , Zea mays/crecimiento & desarrolloRESUMEN
The soils in mining lands with cadmium (Cd) contamination usually are deficient in nutrients. Disclosing how P nutrition and N:P stoichiometric ratio influences Cd accumulation and stress tolerance in stems of Populus spp. will facilitate the phytoremediation of mining sites polluted by Cd. In this study, investigations at the anatomical and physiological levels were conducted using a clone of Populus × euramericana. Both phosphorus deficiency and cadmium exposure inhibited xylem development via reducing cell layers in the xylem. Under P-sufficient condition, appropriate P status and balanced N:P ratio in stem promoted xylem development under Cd exposure via stimulating cell division, which enhanced Cd accumulation in stems. Cd accumulation in cell walls of collenchyma tissues of the stem was enhanced by P application due to increased polysaccharide production and cell wall affinity for Cd. The low P concentrations (0.3-0.4â¯mgâ¯g-1) and imbalanced N:P ratio under P deficiency inhibited the production of APX and ascorbate-GSH cycle, which increased oxidative stress and lipid peroxidation as indicated by high MDA concentration in stem. Under P-sufficient condition, the interactions between phytohormones and antioxidants play crucial roles in the process of antioxidant defense under Cd exposure. In conclusions, appropriate P addition and balanced N:P ratio enhanced secondary xylem development and promoted cadmium accumulation and stress tolerance in Populus stems, which can benefit the phytoextraction of Cd from Cd-contaminated soil.
Asunto(s)
Biodegradación Ambiental , Cadmio/aislamiento & purificación , Fósforo/farmacología , Populus/metabolismo , Xilema/efectos de los fármacos , Antioxidantes/metabolismo , Ácido Ascórbico/metabolismo , Cadmio/metabolismo , Pared Celular/metabolismo , Peroxidación de Lípido/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Fósforo/metabolismo , Suelo/química , Contaminantes del Suelo/aislamiento & purificación , Contaminantes del Suelo/metabolismo , Xilema/crecimiento & desarrolloRESUMEN
In Arabidopsis basal hypocotyls of dark-grown seedlings, xylary cells may form from the pericycle as an alternative to adventitious roots. Several hormones may induce xylogenesis, as Jasmonic acid (JA), as well as indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) auxins, which also affect xylary identity. Studies with the ethylene (ET)-perception mutant ein3eil1 and the ET-precursor 1-aminocyclopropane-1-carboxylic acid (ACC), also demonstrate ET involvement in IBA-induced ectopic metaxylem. Moreover, nitric oxide (NO), produced after IBA/IAA-treatments, may affect JA signalling and interact positively/negatively with ET. To date, NO-involvement in ET/JA-mediated xylogenesis has never been investigated. To study this, and unravel JA-effects on xylary identity, xylogenesis was investigated in hypocotyls of seedlings treated with JA methyl-ester (JAMe) with/without ACC, IBA, IAA. Wild-type (wt) and ein3eil1 responses to hormonal treatments were compared, and the NO signal was quantified and its role evaluated by using NO-donors/scavengers. Ectopic-protoxylem increased in the wt only after treatment with JAMe(10 µM), whereas in ein3eil1 with any JAMe concentration. NO was detected in cells leading to either xylogenesis or adventitious rooting, and increased after treatment with JAMe(10 µM) combined or not with IBA(10 µM). Xylary identity changed when JAMe was applied with each auxin. Altogether, the results show that xylogenesis is induced by JA and NO positively regulates this process. In addition, NO also negatively interacts with ET-signalling and modulates auxin-induced xylary identity.
Asunto(s)
Acetatos/farmacología , Arabidopsis/efectos de los fármacos , Ciclopentanos/farmacología , Ácidos Indolacéticos/farmacología , Óxido Nítrico/metabolismo , Oxilipinas/farmacología , Xilema/efectos de los fármacos , Arabidopsis/genética , Arabidopsis/metabolismo , Etilenos/farmacología , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Hipocótilo/efectos de los fármacos , Hipocótilo/genética , Hipocótilo/metabolismo , Mutación , Reguladores del Crecimiento de las Plantas/farmacología , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/genética , Raíces de Plantas/metabolismo , Plantones/efectos de los fármacos , Plantones/genética , Plantones/metabolismo , Xilema/citología , Xilema/metabolismoRESUMEN
Heavy metal stress changes the morphological and anatomical structure of plant organs. In this study, we determined the anatomical changes and Cd distribution in the roots of Aegiceras corniculatum (L.) Blanco (Black mangrove) under Cd stress. The results showed that Cd levels in A. corniculatum root tissues decreased in the following order: endodermis > pith > xylem > epidermis and exodermis > phloem > cortex. The endodermis secondary casparian strip replaces exodermis casparian strip and plays a role in the "retardation mechanism", which sort of compensates for the missing exodermis retardation effect. The xylem and pith both show high affinity for Cd and contain enriched Cd. This creates a low-Cd environment for phloem and protects the nutrient transport function of the vasculature against Cd toxicity. The present study provides new evidences suggesting that Cd regional enrichment and anatomical structure changes are an adaptive strategy of mangrove plants to HM tolerance.
Asunto(s)
Cadmio/farmacocinética , Cadmio/toxicidad , Raíces de Plantas/efectos de los fármacos , Primulaceae/efectos de los fármacos , Adaptación Biológica/efectos de los fármacos , Cadmio/análisis , Microscopía Electrónica de Rastreo , Epidermis de la Planta/efectos de los fármacos , Epidermis de la Planta/metabolismo , Raíces de Plantas/anatomía & histología , Raíces de Plantas/metabolismo , Primulaceae/anatomía & histología , Primulaceae/metabolismo , Espectrometría por Rayos X , Estrés Fisiológico , Distribución Tisular , Contaminantes Químicos del Agua/farmacocinética , Contaminantes Químicos del Agua/toxicidad , Humedales , Xilema/efectos de los fármacos , Xilema/metabolismoRESUMEN
Auxin is one of the crucial plant hormones which stimulates and controls cell and plant growth. The effects of auxin IBA (indole-3-butyric acid) during 10-days on maize plants growth in controlled conditions (hydroponic, 16-h photoperiod, 70% humidity, 25/20⯰C temperature), depended on its concentration in the substrate. A high concentration (10-7 M) of IBA inhibited root growth, evoked the development of apoplasmic barriers (Casparian bands and suberin lamellae) closer to the root apex, and elevated the amount of lignin in roots. A low concentration (10-11 M) of IBA stimulated root growth but affected neither the development of apoplasmic barriers, nor the amount of lignin. Auxin in a 10-8 M concentration influenced the root growth to a minimal extent compare to the control, and it was the non-effective concentration. Plant cell walls as cell structures ensure cell enlargement and plant growth, and have to react to auxin stimulus by modification of their components. We found the most significant changes in the composition of the PF III fraction (lignocellulosic complex) of the cell wall. The presence of auxin in the substrate affected all three components of this fraction - Klason lignin and both the by acid (2â¯M TFA) non-hydrolysable and the hydrolysable parts of this complex. The ratio of the non-hydrolysable part to the Klason lignin increased from 1.3 to 3.3 with increasing auxin concentrations in the substrate. This may be related to the deposition of polysaccharides and lignin in the cell wall, which help maintain the specific tensile stress of, and turgor pressure on, the cell walls.
Asunto(s)
Indoles/metabolismo , Reguladores del Crecimiento de las Plantas/metabolismo , Zea mays/efectos de los fármacos , Zea mays/fisiología , Pared Celular/efectos de los fármacos , Pared Celular/fisiología , Relación Dosis-Respuesta a Droga , Ácidos Indolacéticos/administración & dosificación , Ácidos Indolacéticos/farmacología , Indoles/administración & dosificación , Lípidos/química , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/fisiología , Xilema/efectos de los fármacos , Xilema/fisiologíaRESUMEN
BACKGROUND: The xylem sap of vascular plants primarily transports water and mineral nutrients from the roots to the shoots and also transports heavy metals such as cadmium (Cd). Proteomic changes in xylem sap is an important mechanism for detoxifying Cd by plants. However, it is unclear how proteins in xylem sap respond to Cd. Here, we investigated the effects of Cd stress on the xylem sap proteome of Brassica napus using a label-free shotgun proteomic approach to elucidate plant response mechanisms to Cd toxicity. RESULTS: We identified and quantified 672 proteins; 67% were predicted to be secretory, and 11% (73 proteins) were unique to Cd-treated samples. Cd stress caused statistically significant and biologically relevant abundance changes in 28 xylem sap proteins. Among these proteins, the metabolic pathways that were most affected were related to cell wall modifications, stress/oxidoreductases, and lipid and protein metabolism. We functionally validated a plant defensin-like protein, BnPDFL, which belongs to the stress/oxidoreductase category, that was unique to the Cd-treated samples and played a positive role in Cd tolerance. Subcellular localization analysis revealed that BnPDFL is cell wall-localized. In vitro Cd-binding assays revealed that BnPDFL has Cd-chelating activity. BnPDFL heterologous overexpression significantly enhanced Cd tolerance in E. coli and Arabidopsis. Functional disruption of Arabidopsis plant defensin genes AtPDF2.3 and AtPDF2.2, which are mainly expressed in root vascular bundles, significantly decreased Cd tolerance. CONCLUSIONS: Several xylem sap proteins in Brassica napus are differentially induced in response to Cd treatment, and plant defensin plays a positive role in Cd tolerance.
Asunto(s)
Brassica napus/genética , Cadmio/efectos adversos , Proteoma/efectos de los fármacos , Contaminantes del Suelo/efectos adversos , Xilema/fisiología , Brassica napus/efectos de los fármacos , Brassica napus/metabolismo , Proteoma/genética , Proteoma/metabolismo , Xilema/efectos de los fármacosRESUMEN
Cadmium (Cd) is absorbed readily by rice plants and is transferred to humans when contaminated rice is consumed. Adding selenium (Se) to the plant nutrient solutions reduces the accumulation of Cd in the rice (Oryza sativa L.) seedlings. However, as the relevant underlying mechanism remains unclear, the aim of our study was to improve our understanding of the Se-mediated resistance to Cd stress in rice. We conducted hydroponic experiments to study the effects of selenite or selenate on Cd subcellular distribution and xylem transport in rice seedlings under Cd stress, and we investigated the antioxidative defense responses in the rice plants. We found that the supplementation of both Se forms decreased the Cd accumulations in the roots and shoots of the rice plants. The selenite addition significantly decreased the Cd contents in different subcellular fractions of the rice roots, increased the proportion of Cd distributed to soluble cytosol by 23.41%, and decreased the Cd distribution in the organelle by 28.74% in contrast with the treatment with Cd only. As regards the selenate addition, only the Cd distribution ratio of cytosol was increased by 13.07%. After adding selenite, a decrease of 55.86% in the Cd concentration in xylem sap was observed, whereas little change was found after treatment co-applied with selenate. The hydrogen peroxide (H2O2) and malondialdehyde(MDA) contents in the rice roots were elevated under Cd stress, and the addition of selenite and selenate decreased the H2O2 levels by 77.78% and 59.26%, respectively. Co-exposure to Cd and Se elevated the glutathione (GSH) accumulations in the rice shoots and roots, with the degree of increase being the following: co-applied with selenite > co-applied with selenate > Cd alone treatment. Exposure to Cd increased the catalase (CAT) activity in the roots significantly, whereas it decreased in the shoots. After selenite or selenate supplementation, the CAT activity in the rice roots increased compared with applying only Cd. Compared with the control, the addition of Cd or Se had no significant effect on the activities of peroxidase (POD) or ascorbate peroxidase (APX). Our results showed that Se affected the Cd accumulation in rice seedlings by altering the Cd subcellular distribution and decreasing the ROS induced by Cd stress. Such effects were more significant in the selenite than in the selenate applied treatment.
Asunto(s)
Cadmio/farmacocinética , Oryza/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Selenio/farmacología , Antioxidantes/metabolismo , Cadmio/toxicidad , Glutatión/metabolismo , Peróxido de Hidrógeno/metabolismo , Hidroponía , Malondialdehído/farmacología , Oryza/metabolismo , Proteínas de Plantas/metabolismo , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/metabolismo , Plantones/efectos de los fármacos , Plantones/metabolismo , Ácido Selénico/farmacología , Ácido Selenioso/farmacología , Distribución Tisular , Xilema/efectos de los fármacos , Xilema/metabolismoRESUMEN
Wood production in fast-growing Eucalyptus grandis trees is highly dependent on both potassium (K) fertilization and water availability but the molecular processes underlying wood formation in response to the combined effects of these two limiting factors remain unknown. E. grandis trees were submitted to four combinations of K-fertilization and water supply. Weighted gene co-expression network analysis and MixOmics-based co-regulation networks were used to integrate xylem transcriptome, metabolome and complex wood traits. Functional characterization of a candidate gene was performed in transgenic E. grandis hairy roots. This integrated network-based approach enabled us to identify meaningful biological processes and regulators impacted by K-fertilization and/or water limitation. It revealed that modules of co-regulated genes and metabolites strongly correlated to wood complex traits are in the heart of a complex trade-off between biomass production and stress responses. Nested in these modules, potential new cell-wall regulators were identified, as further confirmed by the functional characterization of EgMYB137. These findings provide new insights into the regulatory mechanisms of wood formation under stressful conditions, pointing out both known and new regulators co-opted by K-fertilization and/or water limitation that may potentially promote adaptive wood traits.
Asunto(s)
Eucalyptus/crecimiento & desarrollo , Potasio/farmacología , Biología de Sistemas , Árboles/crecimiento & desarrollo , Agua/farmacología , Madera/crecimiento & desarrollo , Biomasa , Pared Celular/efectos de los fármacos , Pared Celular/metabolismo , Eucalyptus/efectos de los fármacos , Redes Reguladoras de Genes/efectos de los fármacos , Metaboloma/efectos de los fármacos , Fenotipo , Proteínas de Plantas/metabolismo , Factores de Transcripción/metabolismo , Transcriptoma/genética , Árboles/efectos de los fármacos , Madera/efectos de los fármacos , Xilema/efectos de los fármacos , Xilema/genética , Xilema/crecimiento & desarrolloRESUMEN
Melatonin is a multifunctional signaling molecule that regulates broad aspects of responses to environmental stresses in plants. Cadmium (Cd) is a persistent soil contaminant that is toxic to all living organisms. Recent reports have uncovered the protective role of melatonin in alleviating Cd phytotoxicity, but little is known about its regulatory mechanisms in plants. In this study, we found that foliar application of melatonin (in particular 100 µmol L-1) remarkably enhanced Cd tolerance of tobacco (Nicotiana tabacum L.) leaves, as evidenced by less Cd accumulation and alleviation of growth inhibition and photoinhibition, compared with nontreated Cd-stressed plants. The addition of melatonin also controlled oxidative damage of Cd on tobacco through direct scavenging and by enhancing the activities of antioxidative enzymes. Melatonin application promoted Cd sequestration in the cell wall and vacuoles based on the analysis of subcellular distribution of Cd in tobacco cells. Structural equation modeling (SEM) analysis revealed that melatonin-induced Cd tolerance in tobacco leaves was modulated by the expression of Cd-transport genes. Molecular evidence illustrated that modulation of IRT1, Nramp1, HMA2, HMA4, and HMA3 genes caused by melatonin could be responsible for weakening Cd uptake, Cd transportation to xylem, and intensifying Cd sequestration into the root vacuoles.
Asunto(s)
Cadmio/toxicidad , Melatonina/farmacología , Nicotiana/efectos de los fármacos , Antioxidantes/farmacología , Regulación de la Expresión Génica de las Plantas , Estrés Oxidativo/efectos de los fármacos , Desarrollo de la Planta/efectos de los fármacos , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Estrés Fisiológico , Nicotiana/genética , Nicotiana/metabolismo , Vacuolas/efectos de los fármacos , Vacuolas/metabolismo , Xilema/efectos de los fármacos , Xilema/metabolismoRESUMEN
Wood formation in higher plants is a complex and costly developmental process regulated by a complex network of transcription factors, short peptide signals and hormones. Correct spatiotemporal initiation of differentiation and downstream developmental stages is vital for proper wood formation. Members of the NAC (NAM, ATAF1/2 and CUC) family of transcription factors are described as top level regulators of xylem cell fate and secondary cell wall (SCW) deposition, but the signals initiating their transcription have yet to be elucidated. We found that treatment of Populus stems with auxin repressed transcription of NAC transcription factors associated with fiber and SCW formation and induced vessel-specific NACs, whereas gibberellic acid (GA) induced the expression of both classes of NAC domain transcription factors involved in wood formation. These transcriptional changes were reflected in alterations of stem anatomy, i.e. auxin treatment reduced cell wall thickness, whereas GA had a promotive effect on SCW deposition and on the rate of wood formation. Similar changes were observed on treatment of Arabidopsis thaliana stems with GA or the synthetic auxin NAA. We also observed corresponding changes in PIN5 overexpressing lines, where interference with auxin transport leads to premature SCW deposition and formation of additional fiber bundles. Together, this suggests wood formation is regulated by an integrated readout of both auxin and GA, which, in turn, controls expression of fiber and vessel specific NACs.
Asunto(s)
Ácidos Indolacéticos/farmacología , Madera/metabolismo , Xilema/efectos de los fármacos , Xilema/metabolismo , Arabidopsis/efectos de los fármacos , Arabidopsis/metabolismo , Pared Celular/efectos de los fármacos , Pared Celular/metabolismo , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Giberelinas/farmacología , Reguladores del Crecimiento de las Plantas/farmacología , Proteínas de Plantas/metabolismo , Factores de Transcripción/metabolismoRESUMEN
Physiological properties involved in cadmium (Cd) transport were investigated in the high Cd accumulating rice line (Lu527-8) in comparison with the normal rice line (Lu527-4) through a soil culture experiment. The results showed that Cd contents in xylem saps of Lu527-8 were 1.68-2.55 times higher than those of Lu527-4 under Cd stress. A high-positive correlation between Cd contents in xylem saps and Cd contents in shoots was observed. Lu527-8 owned a more rapid and effective transport of Cd to above-ground part. By analyzing the relationship between inorganic anions, organic components and Cd contents in xylem saps, the lower HPO42- and oxalate contents were considered to be related to the higher Cd transport in xylem sap of Lu527-8. As for citrate, tartaric and histidine content, significant increases were observed with the increasing Cd contents in xylem saps of two rice lines, and their contents of Lu527-8 were significantly higher than those of Lu527-4. Citrate, tartaric and histidine could take part in root-to-shoot Cd transport in xylem.
Asunto(s)
Cadmio/análisis , Oryza/química , Contaminantes del Suelo/análisis , Xilema/química , Ácido Cítrico/química , Histidina/química , Oryza/efectos de los fármacos , Oxalatos/química , Fosfatos/química , Desarrollo de la Planta/efectos de los fármacos , Raíces de Plantas/química , Raíces de Plantas/efectos de los fármacos , Suelo/química , Estrés Fisiológico/efectos de los fármacos , Tartratos/química , Xilema/efectos de los fármacosRESUMEN
Maize was domesticated from Balsas teosinte c. 10 000 yr ago. Previous studies have suggested that increased tolerance to environmental stress occurred during maize domestication. However, the underlying genetic basis remains largely unknown. We used a maize (W22)-teosinte recombinant inbred line (RIL) to investigate the salt wild-type tolerance aspects of maize domestication. We revealed that ZmHKT2 is a major QTL that regulates K+ homeostasis in saline soils. ZmHKT2 encodes a K+ -preferring HKT family transporter and probably reduces shoot K+ content by removing K+ ions from root-to-shoot flowing xylem sap, ZmHKT2 deficiency increases xylem sap and shoot K+ concentrations, and increases salt tolerance. A coding sequence polymorphism in the ZmHKT2W22 allele (SNP389-G) confers an amino acid variant ZmHKT2 that increases xylem sap K+ concentration, thereby increasing shoot K+ content and salt tolerance. Additional analyses showed that SNP389-G first existed in teosinte (allele frequency 56% in assayed accessions), then swept through the maize population (allele frequency 98%), and that SNP389-G probably underwent positive selection during maize domestication. We conclude that a domestication-associated reduction in K+ transport activity in ZmHKT2 underlies maize shoot K+ content and salt tolerance, and propose that CRISPR-based editing of ZmHKT2 might provide a feasible strategy for improving maize salt tolerance.