ABSTRACT
Multidrug resistance (MDR) enables cancer cells to escape cytotoxic insults of anticancer drugs. Rapid identification of cells exhibiting the MDR phenotype is very important since it can lead to an effective and individual patient based treatment plan. We have investigated a combined vibrational spectroscopic approach, using both micro-Raman and FTIR techniques, in order to characterise a sensitive human uterine sarcoma cell line MES-SA and its multidrug-resistant derivative Garf. In this study, these two complementary methods have been evaluated via the use of principal components analysis (PCA), for discrimination of cells exhibiting the MDR phenotype. Our results indicate that, though they inherently have different sensitivities, both Raman and IR methods can provide a good differentiation of cell phenotypes.
Subject(s)
Drug Resistance, Neoplasm , Sarcoma/chemistry , Uterine Neoplasms/chemistry , Cell Line, Tumor , Female , Humans , Phenotype , Spectroscopy, Fourier Transform Infrared/methods , Spectrum Analysis, Raman/methodsABSTRACT
Preservatives used in the Agro-food industries may be of natural origin or obtained chemically. Because of the increasing interest of consumers in food products that contain only natural ingredients, studies on preservative molecules of natural origin, such as organic acids or peptides, have been reported in the past several years. Such studies, which require numerous assays, may be limited by the large amount of molecules required. Microscale assays provide an opportunity for testing natural components available in low quantity. This study examined a rapid method that used microplates for the evaluation of anti-microbial substances. The method was validated using five foodborne pathogens. It required a low amount of product and was convenient for the determination of correlations between the bacterial growth inhibition and concentration of the antimicrobial substance.
Subject(s)
Anti-Bacterial Agents/pharmacology , Bacteria/drug effects , Food Microbiology , Food Preservation/methods , Food Preservatives/pharmacology , Bacteria/growth & development , Dose-Response Relationship, Drug , Escherichia coli/drug effects , Escherichia coli/growth & development , Listeria/drug effects , Listeria/growth & development , Listeria monocytogenes/drug effects , Listeria monocytogenes/growth & development , Microbial Sensitivity Tests , Plant Extracts/pharmacology , Pseudomonas aeruginosa/drug effects , Pseudomonas aeruginosa/growth & development , Salmonella typhimurium/drug effects , Salmonella typhimurium/growth & development , Staphylococcus aureus/drug effects , Staphylococcus aureus/growth & developmentABSTRACT
Sinorhizobium meliloti growth is affected when the incubation temperature is lower than 22 degrees C. In culture media containing glucose or fructose (1%, w/v), the doubling time at 19 degrees C was about 6.25 h during the exponential growth phase, while it was 2.75 h at 30 degrees C; at 17 degrees C it was three-fold higher than at 30 degrees C. Modifications in the bacterial metabolism explain the doubling time increase when bacteria are incubated at low temperature. We determine here, the phosphoenolpyruvate carboxykinase (PEPCK) activity increases when S. meliloti cells first grown at 30 degrees C are shifted at 17 degrees C and incubated for 10 h at this low temperature; we noted the PEPCK activity was three-fold higher in cells incubated in media containing glucose and shifted from 30 to 17 degrees C than in cells maintained at 30 degrees C, while it was only 1.5-fold higher in cells grown in media containing fructose.