ABSTRACT
Cutaneous leishmaniasis (CL) treatment is based on therapy with Glucantime® , yet, there are few laboratory methods to monitor its success. In this study, ex vivo and in vitro evaluations of peripheral blood monocytes were performed in a longitudinal study to characterize the impact of Glucantime® on overall phenotypic/functional features of these cells from CL patients to identify predictive biomarkers for post-therapeutic monitoring by flow cytometry. The ex vivo evaluation from CL patients demonstrated a modulatory profile before treatment, with a decrease in TLR-2, FcγRII, HLA-DR, CD86, IFN-γR, TNF, IL-12, NO, and an increase in FcγRIII and IL-10R. Conversely, treatment changes some of these biomarker expressions by decreasing FcγRIII and IL-10R and increasing IFN-γR, IL-12 and NO. Moreover, an in vitro analysis of these patients showed a reduced phagocytic capacity of Leishmania braziliensis and higher levels of IL-10 and TGF-ß modulating functional profile. Regardless of the compromised L. braziliensis phagocytic capacity, treatment re-established the production of IL-12, IL-10, TGF-ß and NO at the basal level. Notably, monocytes from patients with early cicatrization showed enhanced FcγRI and FcγRII expressions and reduced IL-10, which was further corroborated by a baseline fold change analysis. Finally, the logistic regression model emphasized the performance of FcγRI, FcγRII and IL-10 as robust predictive biomarkers for post-therapeutic cicatrization during cutaneous leishmaniasis.
Subject(s)
Biomarkers/analysis , Leishmania braziliensis/immunology , Leishmaniasis, Cutaneous/immunology , Receptors, IgG/analysis , Adult , Cicatrix , Cytokines/analysis , Female , Flow Cytometry , Humans , Interleukin-10/analysis , Leishmaniasis, Cutaneous/drug therapy , Leishmaniasis, Cutaneous/parasitology , Leishmaniasis, Cutaneous/pathology , Logistic Models , Longitudinal Studies , Male , Middle Aged , Monocytes/immunology , Young AdultABSTRACT
BACKGROUND AND OBJECTIVE: This study aimed to assess the effect of multiple sessions of a low-level laser therapy (LLLT) adjuvant to scaling and root planing (SRP) on the treatment of experimental periodontitis (EP) in rats treated with 5-fluorouracil (5-FU). MATERIAL AND METHODS: A total of 120 rats were divided into five groups: no treatment (NT); treatment with 5-FU (60 and 40 mg/kg) and no local periodontal treatment (5FU); treatment with 5-FU and SRP (5FU-SRP); treatment with 5-FU, SRP and one LLLT session (660 nm; 0.035 W; 4.2 J; 120 s) (5FU-SRP-1LLLT); and treatment with 5-FU, SRP and four LLLT sessions (0, 24, 48 and 72 h) (5FU-SRP-4LLLT). EP was induced in the mandibular molars through ligature placement. The alveolar bone loss (ABL) area in the furcation region was analysed histometrically. TRAP, proliferating cell nuclear antigen, RANKL, osteoprotegerin and activated caspase-3 patterns were analysed by immunolabeling. Prostaglandin E2 was quantified using an ELISA, and tumour necrosis factor α and interleukin-6 were assessed using the multiplex method. The prevalence rates of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella nigrescens, Prevotella intermedia and Fusobacterium nucleatum were assessed using the PCR method. The data were subjected to statistical analysis (α = 5%). RESULTS: 5FU, 5FU-SRP and 5FU-SRP-1LLLT treatment groups showed higher ABL compared with the NT group (p < 0.05), whereas the 5FU-SRP-4LLLT group showed lower ABL compared with the 5FU group on day 7 and decreased RANKL immunolabeling (p < 0.05). CONCLUSION: Treatment with 5-FU worsened EP, and multiple LLLT sessions adjuvant to SRP seemed to improve periodontitis in rats subjected to 5-FU chemotherapy.
Subject(s)
Drug Therapy/methods , Fluorouracil/pharmacology , Fluorouracil/therapeutic use , Low-Level Light Therapy/methods , Periodontitis/therapy , Alveolar Bone Loss/pathology , Alveolar Bone Loss/therapy , Animals , Bacteria/isolation & purification , Caspase 3/analysis , Combined Modality Therapy , Dental Scaling/methods , Dinoprostone/analysis , Inflammation/pathology , Interleukin-6/analysis , Male , Mandible , Molar , Osteoprotegerin/analysis , Periodontitis/microbiology , Periodontitis/pathology , RANK Ligand/analysis , Rats , Rats, Wistar , Root Planing/methods , Tumor Necrosis Factor-alpha/analysisABSTRACT
INTRODUCTION: Previous cross-sectional studies have shown a high prevalence of chronic disease and disability among the elderly. Given Brazil's rapid aging process and the obvious consequences of the growing number of old people with chronic diseases and associated disabilities for the provision of health services, a need was felt for a study that would overcome the limitations of cross-sectional data and shed some light on the main factors determining whether a person will live longer and free of disabling diseases, the so-called successful aging. The methodology of the first follow-up study of elderly residents in Brazil is presented. METHOD: The profile of the initial cohort is compared with previous cross-sectional data and an in-depth analysis of nonresponse is carried out in order to assess the validity of future longitudinal analysis. The EPIDOSO ('Epidemiologia do Idoso') Study conducted a two-year follow-up of 1,667 elderly people (65+), living in S. Paulo. The study consisted of two waves, each consisting of household, clinical, and biochemical surveys. RESULTS AND CONCLUSIONS: In general, the initial cohort showed a similar profile to previous cross-sectional samples in S. Paulo. There was a majority of women, mostly widows, living in multigenerational households, and a high prevalence of chronic illnesses, psychiatric disturbances, and physical disabilities. Despite all the difficulties inherent in follow-up studies, there was a fairly low rate of nonresponse to the household survey after two years, which did not actually affect the representation of the cohort at the final household assessment, making unbiased longitudinal analysis possible. Concerning the clinical and blood sampling surveys, the respondents tended to be younger and less disabled than the nonrespondents, limiting the use of the clinical and laboratory data to longitudinal analysis aimed at a healthier cohort. It is worth mentioning that gender, education, family support, and socioeconomic status were not important determinants of nonresponse, as is often the case.
Subject(s)
Chronic Disease/epidemiology , Frail Elderly , Aged , Aged, 80 and over , Brazil/epidemiology , Cohort Studies , Female , Follow-Up Studies , Humans , Interviews as Topic , Longitudinal Studies , Male , MethodsABSTRACT
O envolvimento cardíaco ocorre em 25 'por cento' a 50 'por cento' dos pacientes com S.I.D.A., quando analisados por ecocardiografia, biópsia miocárdica ou necrópsia. As manifestaçöes clínicas afetam 10 'por cento' dos pacientes, sendo a insuficiência cardíaca a mais frequente. Descrevemos a evolução de um paciente que tinha teste positivo para H.I.V. e miocardite como a primeira manifestação clínica da doença - a miocardite estava associada com arritmias complexas e bloqueio atrioventricular, havendo necessidade de implante de um marcapasso definitivo.
Subject(s)
Humans , Male , Adult , Myocarditis/complications , Myocarditis/diagnosis , Acquired Immunodeficiency Syndrome , Albuterol/administration & dosage , Homosexuality, Male/history , Methylprednisolone/administration & dosageABSTRACT
Sunflower plants, cv Anhandy, freely visited by insects (control) were compared to plants protected from insects. Two flower isolation methods were used: covering the plants with net cages (width 3m, lenght 4m, height 2m) and bagging the flowers with gauze. The number, the weight and the percentage of seed setting were significantly higher (P 0.05) in the visited plants (579.28; 41.17g and 82.37%; 456.97; 23.44g 79.70%, in two trials) than in plants protected by cages (81.53; 2.23g and 1.16% in the first trial and 111.92; 3.16g e 28.49%, in the second). The number and the weight of no seed settings were significantly higher (P 0.05) in the plants covered with cages (416.29 and 6.58g; 375.25 and 5.04g in two trials) than in the control (142.69 and 1.71g; 182.38 and 2.53g). The percentage of seed setting in the bagged flowers with gauze was intermediate to the control plants and the covered plants. (64.56% in the first trial and 68.61%, in the second). It is concluded that the sunflower, cv Anhandy, presents a significant increase of seed production due to visits of pollinating insects, mainly honey bees. Cage is the most indicated method to protect plants from pollinating insects.
Foram realizados dois ensaios nos quais compararam-se plantas de girassol, do cultivar Anhandy, livremente visitadas por insetos(testemunhas) e outras impedidas de visitação por dois métodos (cobertura de plantas com gaiolas teladas, de 3m de largura, 4m de comprimento e 2m de altura, e ensacamento de capítulos com filó). O número, o peso e a porcentagem de sementes granadas foram significativamente maiores (P 0,05) nas plantas visitadas (579,28; 41,17g e 82,37%; 456,97; 23,44g 79,70%, nos dois ensaios), em relação às plantas cobertas com gaiolas teladas (81,53; 2,23g e 1,16% no primeiro ensaio e 111,92; 3,16g e 28,49%, no segundo). O número e o peso de sementes não granadas foram significativamente maiores (P 0,05) nas plantas cobertas com gaiolas teladas (416,29 e 6,58g; 375,25 e 5,04g, nos dois ensaios) em relação à testemunha (142,69 e 1,71g; 182,38 e 2,53g). Os capítulos ensacados com filó apresentaram valores de porcentagem de sementes granadas intermediários entre as plantas testemunhas e as cobertas com gaiolas teladas (64,56 no primeiro ensaio e 68,61, no segundo). Pode-se concluir que o cultivar de girassol em estudo apresenta significativo aumento de produção de sementes devido a visitas de insetos polinizadores, principalmente abelhas, e que as gaiolas teladas são mais indicadas como método para impedir o acesso de insetos polinizadores.
ABSTRACT
Sunflower plants, cv Anhandy, freely visited by insects (control) were compared to plants protected from insects. Two flower isolation methods were used: covering the plants with net cages (width 3m, lenght 4m, height 2m) and bagging the flowers with gauze. The number, the weight and the percentage of seed setting were significantly higher (P 0.05) in the visited plants (579.28; 41.17g and 82.37%; 456.97; 23.44g 79.70%, in two trials) than in plants protected by cages (81.53; 2.23g and 1.16% in the first trial and 111.92; 3.16g e 28.49%, in the second). The number and the weight of no seed settings were significantly higher (P 0.05) in the plants covered with cages (416.29 and 6.58g; 375.25 and 5.04g in two trials) than in the control (142.69 and 1.71g; 182.38 and 2.53g). The percentage of seed setting in the bagged flowers with gauze was intermediate to the control plants and the covered plants. (64.56% in the first trial and 68.61%, in the second). It is concluded that the sunflower, cv Anhandy, presents a significant increase of seed production due to visits of pollinating insects, mainly honey bees. Cage is the most indicated method to protect plants from pollinating insects.
Foram realizados dois ensaios nos quais compararam-se plantas de girassol, do cultivar Anhandy, livremente visitadas por insetos(testemunhas) e outras impedidas de visitação por dois métodos (cobertura de plantas com gaiolas teladas, de 3m de largura, 4m de comprimento e 2m de altura, e ensacamento de capítulos com filó). O número, o peso e a porcentagem de sementes granadas foram significativamente maiores (P 0,05) nas plantas visitadas (579,28; 41,17g e 82,37%; 456,97; 23,44g 79,70%, nos dois ensaios), em relação às plantas cobertas com gaiolas teladas (81,53; 2,23g e 1,16% no primeiro ensaio e 111,92; 3,16g e 28,49%, no segundo). O número e o peso de sementes não granadas foram significativamente maiores (P 0,05) nas plantas cobertas com gaiolas teladas (416,29 e 6,58g; 375,25 e 5,04g, nos dois ensaios) em relação à testemunha (142,69 e 1,71g; 182,38 e 2,53g). Os capítulos ensacados com filó apresentaram valores de porcentagem de sementes granadas intermediários entre as plantas testemunhas e as cobertas com gaiolas teladas (64,56 no primeiro ensaio e 68,61, no segundo). Pode-se concluir que o cultivar de girassol em estudo apresenta significativo aumento de produção de sementes devido a visitas de insetos polinizadores, principalmente abelhas, e que as gaiolas teladas são mais indicadas como método para impedir o acesso de insetos polinizadores.
ABSTRACT
1. Two proteoglycans, PG1 and PG2, have been isolated from shark cartilage. Both are highly polydisperse and large (molecular mass: 1-10 x 10(6) Daltons) and contain chondroitin sulfate and keratan sulfate side chains, but PG2 is somewhat smaller than PG1 and contains less keratan sulfate. 2. Monoclonal antibodies were raised against PG1. Many antibodies were obtained and one of them, MST1, was subcloned and further characterized. This monoclonal antibody reacts with PG1 and PG2 from shark cartilage and also with aggrecan from bovine trachea cartilage. Chondroitinase AC-treated proteoglycans react with MST1, indicating that the antibody does not recognize chondroitin sulfate. MST1 also recognizes aggrecan from human cartilage and a proteoglycan from bovine brain (neurocan) but it does not recognize proteoglycans from rat Walker tumor, fetal calf muscle and decorin from human myoma. 3. Using MST1 we were able to demonstrate that both PG1 and PG2 aggregate with hyaluronic acid.
Subject(s)
Antibodies, Monoclonal/isolation & purification , Cartilage/chemistry , Proteoglycans/chemistry , Animals , Cattle , Chondroitin Sulfates/chemistry , Chondroitin Sulfates/immunology , Chondroitin Sulfates/isolation & purification , Epitopes , Humans , Keratan Sulfate/chemistry , Keratan Sulfate/immunology , Keratan Sulfate/isolation & purification , Male , Mice , Mice, Inbred BALB C , Proteoglycans/immunology , Proteoglycans/isolation & purification , Rabbits , Rats , SharksABSTRACT
1. Two proteoglycans, PG1 and PG2, have been isolated from shark cartilage. Both are highly polydisperse and large (molecular mass: 1-10 x 10**6 Daltons) and contain chondroitin sulfate and keratan sulfate side chains, but PG2 is somewhat smaller tham PG1 and contains less keratan sulfate. 2. Monoclonal antibodies were raised against PG1. Many antibodies were obtained and one of them, MST1, was subcloned and furter characterized. This monoclonal antibody reacts with PG1 and PG2 from shark cartilage and also with aggrecan from bovine trachea cartilage. Chondroitinase AC-treated proteglycans react MST1, indicating that the antibody does not reconize chondroitin sulfate. MST1 also recognizes aggrecan from human cartilage and a proteoglycan from bovine brain (neurocan) but it does reconize proteoglycans from rat Walker tumor, fetal calf muscle and decorin from human myoma. 3. Using MST1 we were able to demonstrate that both PG1 aggregate with hyaluronic acid
Subject(s)
Cattle , Mice , Rabbits , Rats , Humans , Animals , Male , Antibodies, Monoclonal/isolation & purification , Cartilage/chemistry , Proteoglycans/chemistry , Antibodies, Monoclonal/biosynthesis , Antibodies, Monoclonal/immunology , Chondroitinsulfatases/chemistry , Chondroitinsulfatases/immunology , Chondroitinsulfatases/isolation & purification , Epitopes , Keratan Sulfate/chemistry , Keratan Sulfate/immunology , Keratan Sulfate/isolation & purification , Proteoglycans/immunology , Proteoglycans/isolation & purificationABSTRACT
A parathyroid cyst is a rare disease. A 48 year old man with a nonfunctioning parathyroid cyst is described. The cyst was initially misdiagnosed and taken for a thyroid cyst based on physical examination, thyroid scan, sonography and needle biopsy. Cervical exploration was performed due to the recurrence of the lesion. The cyst was resected and histology showed it to be a parathyroid cyst. Pre and post operative clinical aspects are discussed.
Subject(s)
Parathyroid Diseases/diagnosis , Thyroid Neoplasms/diagnosis , Cysts/diagnosis , Cysts/pathology , Diagnosis, Differential , Humans , Male , Middle Aged , Parathyroid Diseases/pathology , RecurrenceSubject(s)
Methylprednisolone/therapeutic use , Myocarditis/drug therapy , Rheumatic Fever , Rheumatic Heart Disease/drug therapy , Adolescent , Adult , Antibodies, Bacterial/immunology , Female , Humans , Injections, Intravenous , Male , Methylprednisolone/administration & dosage , Myocarditis/immunology , Rheumatic Fever/immunology , Streptococcus/immunologySubject(s)
Infant, Newborn , Infant , Child, Preschool , Child , Adolescent , Adult , Middle Aged , Humans , Male , Endocarditis, BacterialSubject(s)
Adolescent , Adult , Humans , Male , Female , Heart Failure , Methylprednisolone , Rheumatic FeverABSTRACT
Oito pacientes com cardite reumatica ativa a maioria em franca insuficiencia cardiaca foram tratados com esquema de pulsoterapia (lg de metilprednisolona intravenosa). Foram acompanhados por exame clinico,eletrocardiograma, radiografia de torax, provas de atividade reumatica e ecocardiograma. A melhora da insuficiencia cardiaca ocorreu em dias. Esta forma de terapeutica tornou possivel o controle da insuficiencia cardiaca, certamente imunologica em curto periodo de tempo e sem efeitos adversos. Ela permitira, tambem, maior abrangencia em situacoes especiais de insuficiencia cardiaca auto-imune