ABSTRACT
A Streptomyces sp. strain named C/33-6, previously isolated from soil, presented a strong and specific antagonistic effect against toxigenic fungi. This action was attributed to a proteinaceous compound (molecular weight estimated to be 14 kDa) present in the supernatant of the culture of strain C/33-6, which was sensitive to proteinases (elastase, pronase E, proteinase K) and prolongated heat treatment (100 degrees C, for 20 min). This compound showed non-chitinolytic fungicidal activity.
Subject(s)
Bacterial Proteins/isolation & purification , Bacterial Proteins/pharmacology , Fungi/drug effects , Growth Inhibitors/isolation & purification , Growth Inhibitors/pharmacology , Streptomyces/chemistry , Antifungal Agents/isolation & purification , Antifungal Agents/metabolism , Antifungal Agents/pharmacology , Bacterial Proteins/metabolism , Chitin/metabolism , Endopeptidase K/metabolism , Fungi/growth & development , Growth Inhibitors/metabolism , Hot Temperature , Hydrogen-Ion Concentration , Microbial Sensitivity Tests , Molecular Weight , Pancreatic Elastase/metabolism , Pronase/metabolism , Protein Denaturation , Streptomyces/metabolismABSTRACT
Mycological and/or bacteriological studies were performed on 294 samples isolated from the external auditory control (EAC). It was observed that 185 (81.5%) of the mycological analysis were negative while 42 (18.5%) were positive with the following distribution: 22 Candida (9.7%), 15 Aspergillus (6.5%) and five associations of Aspergillus and Candida (2.2%). The bacteriological studies were negative in 52 cases (23.6%) and the frequency of the bacterial agents (75.5%) isolated were as follows: 41 Pseudomonas aeruginosa (18.6%), 24 Proteus mirabilis (10.9%), 24 Staphylococcus aureus (10.9%), and three associations of Ps. aeruginosa and Pr. mirabilis (1.4%). Pseudomonas aeruginosa, which was the most frequent bacterial species isolated, did not present associations with any fungus.
Subject(s)
Aspergillus/isolation & purification , Bacteria/isolation & purification , Candida/isolation & purification , Otitis Externa/microbiology , Argentina , Aspergillosis/microbiology , Aspergillus/classification , Bacteria/classification , Bacterial Infections/microbiology , Candida/classification , Candidiasis/microbiology , HumansABSTRACT
We developed a seminested PCR for the diagnosis of histoplasmosis that amplifies a portion of the Histoplasma capsulatum H antigen gene. This assay is highly sensitive and specific, being able to detect genomic material corresponding to less than 10 yeast cells without cross-reaction against other bacterial or fungal pathogens.