Super-resolution multicolor fluorescence microscopy enabled by an apochromatic super-oscillatory lens with extended depth-of-focus.

Planar super-oscillatory lens (SOL), a far-field subwavelength-focusing diffractive device, holds great potential for achieving sub-diffraction-limit imaging at multiple wavelengths. However, conventional SOL devices suffer from a numerical-aperture-related intrinsic tradeoff among the depth of focus (DoF), chromatic dispersion and focusing spot size. Here, we apply a multi-objective genetic algorithm (GA) optimization approach to design an apochromatic binary-phase SOL having a prolonged DoF, customized working distance (WD), minimized main-lobe size, and suppressed side-lobe intensity. Experimental implementation demonstrates simultaneous focusing of blue, green and red light beams into an optical needle of ~0.5λ in diameter and DOF > 10λ at WD = 428 µm. By integrating this SOL device with a commercial fluorescence microscope, we perform, for the first time, three-dimensional super-resolution multicolor fluorescence imaging of the "unseen" fine structures of neurons. The present study provides not only a practical route to far-field multicolor super-resolution imaging but also a viable approach for constructing imaging systems avoiding complex sample positioning and unfavorable photobleaching.

Sidelobe-suppressed sub-diffraction-limit quasi-non-diffracting light sheets achieved by super-oscillatory lenses.

Sub-diffraction-limit quasi-non-diffracting light sheets (SQLSs) are crucial for a resolution-enhanced and field of view (FOV)-enlarged light sheet microscope. However, it has aways been plagued by sidelobes inducing severe background noise. Here, a self-trade-off optimized method is proposed to generate sidelobe-suppressed SQLSs based on super-oscillatory lenses (SOLs). An SQLS thus obtained shows sidelobes of only 15.4%, first realizing the sub-diffraction-limit thickness, quasi-non-diffracting characteristic, and suppressed sidelobes simultaneously for static light sheets. Moreover, a window-like energy allocation is realized by the self-trade-off optimized method, successfully further suppressing the sidelobes. In particular, an SQLS with theoretical sidelobes of 7.6% is achieved within the window, which provides a new strategy to deal with sidelobes for light sheets and shows great potential in high signal-to-noise ratio light sheet microscopy (LSM).

Sub-diffraction-limit light sheet enabled by a super-oscillatory lens with an enlarged field of view and depth of focus.

Static light sheets are widely used in various super-resolution three-dimensional (3D) imaging applications. Here, a multifocal diffraction-free optimized design method is proposed for super-oscillatory lenses (SOLs) owning an enlarged field of view (FOV) to generate sub-diffraction-limit light sheets with reduced divergence. Various propagation lengths of sub-diffraction-limit thickness for light sheets can be obtained by adopting corresponding numbers of discrete foci and spacing between them. In particular, the propagation lengths of 150.4λ and 118.9λ are obtained by SOLs with an enlarged FOV of 150λ and 820λ, respectively, which show the longest depth of focus (DOF), as far as we know, and are the first to realize the combination of enlarged DOF and FOV for SOLs. We show a way of using binary-amplitude modulation to generate static light sheets with sub-diffraction-limit thickness and reduced divergence, which is simple, easy to integrate, and sidelobe-suppressed.